TY  - JOUR
AU  - Đorđević, Sanela
AU  - Santrač, Anja
AU  - Cekić, Nebojša
AU  - Marković, Bojan
AU  - Divović, Branka
AU  - Ilić, Tanja
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2883
AB  - This work aimed to deepen the lately acquired knowledge about parenteral nanoemulsions as carriers for brain delivery of risperidone, a poorly water-soluble antipsychotic drug, through establishing the prospective relationship between their physicochemical, pharmacokinetic, biodistribution, and behavioral performances. For this purpose, two optimized risperidone-loaded nanoemulsions, stabilized by lecithin or lecithin/polysorbate 80 mixture, and costabilized by sodium oleate, were produced by high-pressure homogenization. The characterization revealed the favorable droplet size, narrow size distribution, high surface charge, with proven stability to autoclaving and long-term stability for at least one year at 25 +/- 2 degrees C. Pharmacokinetic and tissue distribution results demonstrated improved plasma, liver, and brain pharmacokinetic parameters, resulting in 1.2-1.5-fold increased relative bioavailability, 1.1-1.8-fold decreased liver distribution, and about 1.3-fold improved brain uptake of risperidone active moiety following intraperitoneal administration of nanoemulsions relative to solution in rats. In behavioral study, investigated nanoemulsions showed pronounced reduction in basal and, more pertinently, amphetamine-induced locomotor activity in rats, with an early onset of antipsychotic action, and this effect lasted at least 90 min after drug injection. Together, these findings corroborate the applicability of parenteral nanoemulsions as carriers for enhanced brain delivery of risperidone, further suggesting their promise in acute psychosis treatment or other emergency situations.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Parenteral nanoemulsions of risperidone for enhanced brain delivery in acute psychosis: Physicochemical and in vivo performances
VL  - 533
IS  - 2
SP  - 421
EP  - 430
DO  - 10.1016/j.ijpharm.2017.05.051
ER  - 

@article{
author = "Đorđević, Sanela and Santrač, Anja and Cekić, Nebojša and Marković, Bojan and Divović, Branka and Ilić, Tanja and Savić, Miroslav and Savić, Snežana",
year = "2017",
abstract = "This work aimed to deepen the lately acquired knowledge about parenteral nanoemulsions as carriers for brain delivery of risperidone, a poorly water-soluble antipsychotic drug, through establishing the prospective relationship between their physicochemical, pharmacokinetic, biodistribution, and behavioral performances. For this purpose, two optimized risperidone-loaded nanoemulsions, stabilized by lecithin or lecithin/polysorbate 80 mixture, and costabilized by sodium oleate, were produced by high-pressure homogenization. The characterization revealed the favorable droplet size, narrow size distribution, high surface charge, with proven stability to autoclaving and long-term stability for at least one year at 25 +/- 2 degrees C. Pharmacokinetic and tissue distribution results demonstrated improved plasma, liver, and brain pharmacokinetic parameters, resulting in 1.2-1.5-fold increased relative bioavailability, 1.1-1.8-fold decreased liver distribution, and about 1.3-fold improved brain uptake of risperidone active moiety following intraperitoneal administration of nanoemulsions relative to solution in rats. In behavioral study, investigated nanoemulsions showed pronounced reduction in basal and, more pertinently, amphetamine-induced locomotor activity in rats, with an early onset of antipsychotic action, and this effect lasted at least 90 min after drug injection. Together, these findings corroborate the applicability of parenteral nanoemulsions as carriers for enhanced brain delivery of risperidone, further suggesting their promise in acute psychosis treatment or other emergency situations.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Parenteral nanoemulsions of risperidone for enhanced brain delivery in acute psychosis: Physicochemical and in vivo performances",
volume = "533",
number = "2",
pages = "421-430",
doi = "10.1016/j.ijpharm.2017.05.051"
}

Đorđević, S., Santrač, A., Cekić, N., Marković, B., Divović, B., Ilić, T., Savić, M.,& Savić, S.. (2017). Parenteral nanoemulsions of risperidone for enhanced brain delivery in acute psychosis: Physicochemical and in vivo performances. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 533(2), 421-430.
https://doi.org/10.1016/j.ijpharm.2017.05.051

Đorđević S, Santrač A, Cekić N, Marković B, Divović B, Ilić T, Savić M, Savić S. Parenteral nanoemulsions of risperidone for enhanced brain delivery in acute psychosis: Physicochemical and in vivo performances. in International Journal of Pharmaceutics. 2017;533(2):421-430.
doi:10.1016/j.ijpharm.2017.05.051 .

Đorđević, Sanela, Santrač, Anja, Cekić, Nebojša, Marković, Bojan, Divović, Branka, Ilić, Tanja, Savić, Miroslav, Savić, Snežana, "Parenteral nanoemulsions of risperidone for enhanced brain delivery in acute psychosis: Physicochemical and in vivo performances" in International Journal of Pharmaceutics, 533, no. 2 (2017):421-430,
https://doi.org/10.1016/j.ijpharm.2017.05.051 . .

TY  - CHAP
AU  - Isailović, Tanja
AU  - Todosijević, Marija N.
AU  - Đorđević, Sanela
AU  - Savić, Snežana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2997
AB  - Considering the superior advantages over traditional formulation approaches, micro/nanoemulsions represent very attractive carriers worth exploring for improving dermal/transdermal delivery of NSAIDs. Currently, natural-origin sucrose esters are of increasing interest for research community, due to their high environmental compatibility, low skin sensitization potential, and skin penetration enhancement. In this context, the present chapter provides an overview of sucrose esters' potential in the development of biocompatible micro/nanoemulsions for topical administration of NSAIDs. The formation and performances of sucrose ester-based micro/nanoemulsions are governed by numerous factors which should be carefully considered, such as (1) chain length and monoester content of the sucrose esters, (2) nature and concentration of the cosurfactant, oil, and aqueous phase, (3) the components' weight ratio, and (4) physicochemical characteristics of NSAID. Therefore, here we review the key researches regarding NSAID-loaded sucrose ester-based micro/nanoemulsions, starting from the formulation design consideration, through physicochemical characterization and dermal/transdermal availability assessment of developed systems.
PB  - Elsevier Inc.
T2  - Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a
T1  - Natural Surfactants-Based Micro/Nanoemulsion Systems for NSAIDs- Practical Formulation Approach, Physicochemical and Biopharmaceutical Characteristics/Performances
SP  - 179
EP  - 217
DO  - 10.1016/B978-0-12-804017-1.00007-8
ER  - 

@inbook{
author = "Isailović, Tanja and Todosijević, Marija N. and Đorđević, Sanela and Savić, Snežana",
year = "2017",
abstract = "Considering the superior advantages over traditional formulation approaches, micro/nanoemulsions represent very attractive carriers worth exploring for improving dermal/transdermal delivery of NSAIDs. Currently, natural-origin sucrose esters are of increasing interest for research community, due to their high environmental compatibility, low skin sensitization potential, and skin penetration enhancement. In this context, the present chapter provides an overview of sucrose esters' potential in the development of biocompatible micro/nanoemulsions for topical administration of NSAIDs. The formation and performances of sucrose ester-based micro/nanoemulsions are governed by numerous factors which should be carefully considered, such as (1) chain length and monoester content of the sucrose esters, (2) nature and concentration of the cosurfactant, oil, and aqueous phase, (3) the components' weight ratio, and (4) physicochemical characteristics of NSAID. Therefore, here we review the key researches regarding NSAID-loaded sucrose ester-based micro/nanoemulsions, starting from the formulation design consideration, through physicochemical characterization and dermal/transdermal availability assessment of developed systems.",
publisher = "Elsevier Inc.",
journal = "Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a",
booktitle = "Natural Surfactants-Based Micro/Nanoemulsion Systems for NSAIDs- Practical Formulation Approach, Physicochemical and Biopharmaceutical Characteristics/Performances",
pages = "179-217",
doi = "10.1016/B978-0-12-804017-1.00007-8"
}

Isailović, T., Todosijević, M. N., Đorđević, S.,& Savić, S.. (2017). Natural Surfactants-Based Micro/Nanoemulsion Systems for NSAIDs- Practical Formulation Approach, Physicochemical and Biopharmaceutical Characteristics/Performances. in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a
Elsevier Inc.., 179-217.
https://doi.org/10.1016/B978-0-12-804017-1.00007-8

Isailović T, Todosijević MN, Đorđević S, Savić S. Natural Surfactants-Based Micro/Nanoemulsion Systems for NSAIDs- Practical Formulation Approach, Physicochemical and Biopharmaceutical Characteristics/Performances. in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a. 2017;:179-217.
doi:10.1016/B978-0-12-804017-1.00007-8 .

Isailović, Tanja, Todosijević, Marija N., Đorđević, Sanela, Savić, Snežana, "Natural Surfactants-Based Micro/Nanoemulsion Systems for NSAIDs- Practical Formulation Approach, Physicochemical and Biopharmaceutical Characteristics/Performances" in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a (2017):179-217,
https://doi.org/10.1016/B978-0-12-804017-1.00007-8 . .

TY  - CHAP
AU  - Milić, Jela
AU  - Čalija, Bojan
AU  - Đorđević, Sanela
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2893
AB  - Continuous efforts to improve performance of drugs via incorporation in particulate and soft colloidal carriers have introduced new functional categories of excipients. These excipients are used to protect drugs, modify their release, and increase their bioavailability and/or selectivity for target tissues with the aim to improve patient compliance, and therapeutic outcomes. Structural and physicochemical variability of these materials may affect their functionality in the final formulation. Therefore, it is of great importance to identify, evaluate, and control physical or chemical characteristics of excipients that are important for their intended use. These characteristics are known as functionality-related characteristics of excipients.This chapter gives an overview of characteristics and variability of the three distinct groups of excipients commonly used for preparation of particulate and soft colloidal carriers: biodegradable polymeric materials, silica-based materials, and natural surfactants. Special attention is paid to functionality, functionality-related characteristics, and functionalization of these materials in relation to their use for preparation of micro/nanosized drug carriers.
PB  - Elsevier Inc.
T2  - Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a
T1  - Diversity and Functionality of Excipients for Micro/Nanosized Drug Carriers
SP  - 95
EP  - 132
DO  - 10.1016/B978-0-12-804017-1.00004-2
ER  - 

@inbook{
author = "Milić, Jela and Čalija, Bojan and Đorđević, Sanela",
year = "2017",
abstract = "Continuous efforts to improve performance of drugs via incorporation in particulate and soft colloidal carriers have introduced new functional categories of excipients. These excipients are used to protect drugs, modify their release, and increase their bioavailability and/or selectivity for target tissues with the aim to improve patient compliance, and therapeutic outcomes. Structural and physicochemical variability of these materials may affect their functionality in the final formulation. Therefore, it is of great importance to identify, evaluate, and control physical or chemical characteristics of excipients that are important for their intended use. These characteristics are known as functionality-related characteristics of excipients.This chapter gives an overview of characteristics and variability of the three distinct groups of excipients commonly used for preparation of particulate and soft colloidal carriers: biodegradable polymeric materials, silica-based materials, and natural surfactants. Special attention is paid to functionality, functionality-related characteristics, and functionalization of these materials in relation to their use for preparation of micro/nanosized drug carriers.",
publisher = "Elsevier Inc.",
journal = "Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a",
booktitle = "Diversity and Functionality of Excipients for Micro/Nanosized Drug Carriers",
pages = "95-132",
doi = "10.1016/B978-0-12-804017-1.00004-2"
}

Milić, J., Čalija, B.,& Đorđević, S.. (2017). Diversity and Functionality of Excipients for Micro/Nanosized Drug Carriers. in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a
Elsevier Inc.., 95-132.
https://doi.org/10.1016/B978-0-12-804017-1.00004-2

Milić J, Čalija B, Đorđević S. Diversity and Functionality of Excipients for Micro/Nanosized Drug Carriers. in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a. 2017;:95-132.
doi:10.1016/B978-0-12-804017-1.00004-2 .

Milić, Jela, Čalija, Bojan, Đorđević, Sanela, "Diversity and Functionality of Excipients for Micro/Nanosized Drug Carriers" in Microsized and Nanosized Carriers for Nonsteroidal Anti-Inflammatory Drugs: Formulation Challenges a (2017):95-132,
https://doi.org/10.1016/B978-0-12-804017-1.00004-2 . .

TY  - JOUR
AU  - Đorđević, Sanela
AU  - Isailović, Tanja
AU  - Cekić, Nebojša
AU  - Vuleta, Gordana
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2731
AB  - The aim of the present study was to develop parenteral nanoemulsions containing increasing content of oil phase (20, 30 and 40%, w/w of medium-chain triglycerides-soybean oil mixture at 4:1 ratio), stabilized by lecithin-polysorbate 80 mixture, and to assess their feasibility as carriers for poorly water-soluble psychopharmacological drugs. To this purpose, nanoemulsions loaded with diazepam as a model drug were prepared through high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, viscosity, pH value, and electrical conductivity. Furthermore, the in vitro release of diazepam from developed nanoemulsions was examined using reverse dialysis bag technique, and drug release kinetics was evaluated through several mathematical models. After preparation, all formulations revealed small mean droplet size (206 ± 7 nm), with narrow size distribution (0.116 ± 0.012) and zeta potential around -50 mV, complying with pharmacopoeial requirements (USP 39-NF 34), wherein there were no significant changes in monitored parameters after one year of storage at 25 ± 2°C. In vitro drug release study demonstrated that 40-50% of diazepam was released from actual nanoemulsions within 1 h, while the kinetic release process could be described by Korsmeyer-Peppas model. The results obtained suggest that formulated parenteral nanoemulsions might be promising carriers for rapid delivery of lipophilic, poorly water-soluble psychopharmacological drugs.
AB  - Cilj ovog istraživanja bio je da se razviju parenteralne nanoemulzije sa rastućom koncentracijom uljane faze (20, 30 i 40% smeše triglicerida srednje dužine lanca i sojinog ulja u odnosu 4:1), stabilizovane kombinacijom lecitina i polisorbata 80, i da se proceni njihova pogodnost kao nosača za slabo rastvorljive psihofarmakološke lekovite supstance. U tu svrhu, homogenizacijom pod visokim pritiskom izrađene su nanoemulzije sa diazepamom kao model lekovitom supstancom i okarakterisane u pogledu veličine kapi, indeksa polidisperznosti, površinskog naelektrisanja, viskoziteta, pH vrednosti i električne provodljivosti. Takođe, primenom reverzne tehnike sa dijaliznim vrećicama procenjena je brzina oslobađanja diazepama iz razvijenih nanoemulzija, uz karakterizaciju dobijenih profila oslobađanja primenom različitih matematičkih modela. Nakon izrade, sve formulacije imale su malu prosečnu veličinu kapi (206 ± 7 nm), sa uskom raspodelom veličina (0,116 ± 0,012) i zeta potencijalom oko -50 mV, što je u skladu sa farmakopejskim zahtevima (USP 39-NF 34) pri čemu se vrednosti navedenih parametara nisu značajno promenile nakon godinu dana čuvanja na 25 ± 2°C. In vitro ispitivanje brzine oslobađanja pokazalo je da se 40-50% diazepama oslobodi iz ispitivanih nanoemulzija tokom 1 h, pri čemu se kinetika oslobađanja može opisati Korsmeyer-Peppas modelom. Dobijeni rezultati ukazuju da formulisane parenteralne nanoemulzije predstavljaju obećavajuće nosače za brzu isporuku slabo rastvorljivih psihofarmakoloških lekovitih supstanci.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Diazepam-loaded parenteral nanoemulsions: Physicochemical characterization and in vitro release study
T1  - Parenteralne nanoemulzije diazepama - fizičkohemijska karakterizacija i in vitro ispitivanje brzine oslobađanja
VL  - 66
IS  - 1
SP  - 24
EP  - 41
DO  - 10.5937/arhfarm1601024D
ER  - 

@article{
author = "Đorđević, Sanela and Isailović, Tanja and Cekić, Nebojša and Vuleta, Gordana and Savić, Snežana",
year = "2016",
abstract = "The aim of the present study was to develop parenteral nanoemulsions containing increasing content of oil phase (20, 30 and 40%, w/w of medium-chain triglycerides-soybean oil mixture at 4:1 ratio), stabilized by lecithin-polysorbate 80 mixture, and to assess their feasibility as carriers for poorly water-soluble psychopharmacological drugs. To this purpose, nanoemulsions loaded with diazepam as a model drug were prepared through high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, viscosity, pH value, and electrical conductivity. Furthermore, the in vitro release of diazepam from developed nanoemulsions was examined using reverse dialysis bag technique, and drug release kinetics was evaluated through several mathematical models. After preparation, all formulations revealed small mean droplet size (206 ± 7 nm), with narrow size distribution (0.116 ± 0.012) and zeta potential around -50 mV, complying with pharmacopoeial requirements (USP 39-NF 34), wherein there were no significant changes in monitored parameters after one year of storage at 25 ± 2°C. In vitro drug release study demonstrated that 40-50% of diazepam was released from actual nanoemulsions within 1 h, while the kinetic release process could be described by Korsmeyer-Peppas model. The results obtained suggest that formulated parenteral nanoemulsions might be promising carriers for rapid delivery of lipophilic, poorly water-soluble psychopharmacological drugs., Cilj ovog istraživanja bio je da se razviju parenteralne nanoemulzije sa rastućom koncentracijom uljane faze (20, 30 i 40% smeše triglicerida srednje dužine lanca i sojinog ulja u odnosu 4:1), stabilizovane kombinacijom lecitina i polisorbata 80, i da se proceni njihova pogodnost kao nosača za slabo rastvorljive psihofarmakološke lekovite supstance. U tu svrhu, homogenizacijom pod visokim pritiskom izrađene su nanoemulzije sa diazepamom kao model lekovitom supstancom i okarakterisane u pogledu veličine kapi, indeksa polidisperznosti, površinskog naelektrisanja, viskoziteta, pH vrednosti i električne provodljivosti. Takođe, primenom reverzne tehnike sa dijaliznim vrećicama procenjena je brzina oslobađanja diazepama iz razvijenih nanoemulzija, uz karakterizaciju dobijenih profila oslobađanja primenom različitih matematičkih modela. Nakon izrade, sve formulacije imale su malu prosečnu veličinu kapi (206 ± 7 nm), sa uskom raspodelom veličina (0,116 ± 0,012) i zeta potencijalom oko -50 mV, što je u skladu sa farmakopejskim zahtevima (USP 39-NF 34) pri čemu se vrednosti navedenih parametara nisu značajno promenile nakon godinu dana čuvanja na 25 ± 2°C. In vitro ispitivanje brzine oslobađanja pokazalo je da se 40-50% diazepama oslobodi iz ispitivanih nanoemulzija tokom 1 h, pri čemu se kinetika oslobađanja može opisati Korsmeyer-Peppas modelom. Dobijeni rezultati ukazuju da formulisane parenteralne nanoemulzije predstavljaju obećavajuće nosače za brzu isporuku slabo rastvorljivih psihofarmakoloških lekovitih supstanci.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Diazepam-loaded parenteral nanoemulsions: Physicochemical characterization and in vitro release study, Parenteralne nanoemulzije diazepama - fizičkohemijska karakterizacija i in vitro ispitivanje brzine oslobađanja",
volume = "66",
number = "1",
pages = "24-41",
doi = "10.5937/arhfarm1601024D"
}

Đorđević, S., Isailović, T., Cekić, N., Vuleta, G.,& Savić, S.. (2016). Diazepam-loaded parenteral nanoemulsions: Physicochemical characterization and in vitro release study. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 66(1), 24-41.
https://doi.org/10.5937/arhfarm1601024D

Đorđević S, Isailović T, Cekić N, Vuleta G, Savić S. Diazepam-loaded parenteral nanoemulsions: Physicochemical characterization and in vitro release study. in Arhiv za farmaciju. 2016;66(1):24-41.
doi:10.5937/arhfarm1601024D .

Đorđević, Sanela, Isailović, Tanja, Cekić, Nebojša, Vuleta, Gordana, Savić, Snežana, "Diazepam-loaded parenteral nanoemulsions: Physicochemical characterization and in vitro release study" in Arhiv za farmaciju, 66, no. 1 (2016):24-41,
https://doi.org/10.5937/arhfarm1601024D . .

TY  - JOUR
AU  - Isailović, Tanja
AU  - Đorđević, Sanela
AU  - Marković, Bojan
AU  - Randelović, Danijela
AU  - Cekić, Nebojša
AU  - Lukić, Milica
AU  - Pantelić, Ivana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2716
AB  - We aimed to develop lecithin-based nanoemulsions intended for effective aceclofenac (ACF) skin delivery utilizing sucrose esters [sucrose palmitate (SP) and sucrose stearate (SS)] as additional stabilizers and penetration enhancers. To find the suitable surfactant mixtures and levels of process variables (homogenization pressure and number of cycles-high pressure homogenization manufacturing method) that result in drug-loaded nanoemulsions with minimal droplet size and narrow size distribution, a combined mixture-process experimental design was employed. Based on optimization data, selected nanoemulsions were evaluated regarding morphology, surface charge, drug-excipient interactions, physical stability, and in vivo skin performances (skin penetration and irritation potential). The predicted physicochemical properties and storage stability were proved satisfying for ACF-loaded nanoemulsions containing 2% of SP in the blend with 0%-1% of SS and 1%-2% of egg lecithin (produced at 50 degrees C/20 cycles/800 bar). Additionally, the in vivo tape stripping demonstrated superior ACF skin absorption from these nanoemulsions, particularly from those containing 2% of SP, 0.5% of SS, and 1.5% of egg lecithin, when comparing with the sample costabilized by conventional surfactant-polysorbate 80. In summary, the combined mixture-process experimental design was shown as a feasible tool for formulation development of multisurfactant-based nanosized delivery systems with potentially improved overall product performances.
PB  - Elsevier Science Inc, New York
T2  - Journal of Pharmaceutical Sciences
T1  - Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design
VL  - 105
IS  - 1
SP  - 308
EP  - 323
DO  - 10.1002/jps.24706
ER  - 

@article{
author = "Isailović, Tanja and Đorđević, Sanela and Marković, Bojan and Randelović, Danijela and Cekić, Nebojša and Lukić, Milica and Pantelić, Ivana and Daniels, Rolf and Savić, Snežana",
year = "2016",
abstract = "We aimed to develop lecithin-based nanoemulsions intended for effective aceclofenac (ACF) skin delivery utilizing sucrose esters [sucrose palmitate (SP) and sucrose stearate (SS)] as additional stabilizers and penetration enhancers. To find the suitable surfactant mixtures and levels of process variables (homogenization pressure and number of cycles-high pressure homogenization manufacturing method) that result in drug-loaded nanoemulsions with minimal droplet size and narrow size distribution, a combined mixture-process experimental design was employed. Based on optimization data, selected nanoemulsions were evaluated regarding morphology, surface charge, drug-excipient interactions, physical stability, and in vivo skin performances (skin penetration and irritation potential). The predicted physicochemical properties and storage stability were proved satisfying for ACF-loaded nanoemulsions containing 2% of SP in the blend with 0%-1% of SS and 1%-2% of egg lecithin (produced at 50 degrees C/20 cycles/800 bar). Additionally, the in vivo tape stripping demonstrated superior ACF skin absorption from these nanoemulsions, particularly from those containing 2% of SP, 0.5% of SS, and 1.5% of egg lecithin, when comparing with the sample costabilized by conventional surfactant-polysorbate 80. In summary, the combined mixture-process experimental design was shown as a feasible tool for formulation development of multisurfactant-based nanosized delivery systems with potentially improved overall product performances.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Pharmaceutical Sciences",
title = "Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design",
volume = "105",
number = "1",
pages = "308-323",
doi = "10.1002/jps.24706"
}

Isailović, T., Đorđević, S., Marković, B., Randelović, D., Cekić, N., Lukić, M., Pantelić, I., Daniels, R.,& Savić, S.. (2016). Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design. in Journal of Pharmaceutical Sciences
Elsevier Science Inc, New York., 105(1), 308-323.
https://doi.org/10.1002/jps.24706

Isailović T, Đorđević S, Marković B, Randelović D, Cekić N, Lukić M, Pantelić I, Daniels R, Savić S. Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design. in Journal of Pharmaceutical Sciences. 2016;105(1):308-323.
doi:10.1002/jps.24706 .

Isailović, Tanja, Đorđević, Sanela, Marković, Bojan, Randelović, Danijela, Cekić, Nebojša, Lukić, Milica, Pantelić, Ivana, Daniels, Rolf, Savić, Snežana, "Biocompatible Nanoemulsions for Improved Aceclofenac Skin Delivery: Formulation Approach Using Combined Mixture-Process Experimental Design" in Journal of Pharmaceutical Sciences, 105, no. 1 (2016):308-323,
https://doi.org/10.1002/jps.24706 . .

TY  - JOUR
AU  - Filipović, Mila
AU  - Lukić, Milica
AU  - Krstonosić, Veljko
AU  - Đorđević, Sanela
AU  - Pantelić, Ivana
AU  - Gledović, Ana
AU  - Vuleta, Gordana
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2527
AB  - During the formulation of liposome-containing products different problems can occur and the selection of a suitable carrier remains the greatest challenge. To estimate feasibility of a novel alkyl polyglucoside surfactant (hydroxystearyl alcohol and hydroxystearyl glucoside) as an emulsifier for cosmetics with liposome-encapsulated plant stem cells, we performed a two-phase study. In the first, the pre-formulation phase, the emulsifier's critical micelle concentration (CMC) and liposome-encapsulated active-emulsifier interactions were determined. The second phase was carried out to develop and characterize a cosmetic emulsion suitable to serve as a carrier for liposomes. The investigated emulsifier, with the obtained CMC value of 0.0085 wt.%, could be classified as liposome-friendly and can be used to develop stable and aesthetically acceptable cosmetics or even prospective pharmaceutical liposome-containing emulsions.
PB  - Carl Hanser Verlag, Munich
T2  - Tenside Surfactants Detergents
T1  - Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies
VL  - 53
IS  - 3
SP  - 214
EP  - 226
DO  - 10.3139/113.110426
ER  - 

@article{
author = "Filipović, Mila and Lukić, Milica and Krstonosić, Veljko and Đorđević, Sanela and Pantelić, Ivana and Gledović, Ana and Vuleta, Gordana and Savić, Snežana",
year = "2016",
abstract = "During the formulation of liposome-containing products different problems can occur and the selection of a suitable carrier remains the greatest challenge. To estimate feasibility of a novel alkyl polyglucoside surfactant (hydroxystearyl alcohol and hydroxystearyl glucoside) as an emulsifier for cosmetics with liposome-encapsulated plant stem cells, we performed a two-phase study. In the first, the pre-formulation phase, the emulsifier's critical micelle concentration (CMC) and liposome-encapsulated active-emulsifier interactions were determined. The second phase was carried out to develop and characterize a cosmetic emulsion suitable to serve as a carrier for liposomes. The investigated emulsifier, with the obtained CMC value of 0.0085 wt.%, could be classified as liposome-friendly and can be used to develop stable and aesthetically acceptable cosmetics or even prospective pharmaceutical liposome-containing emulsions.",
publisher = "Carl Hanser Verlag, Munich",
journal = "Tenside Surfactants Detergents",
title = "Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies",
volume = "53",
number = "3",
pages = "214-226",
doi = "10.3139/113.110426"
}

Filipović, M., Lukić, M., Krstonosić, V., Đorđević, S., Pantelić, I., Gledović, A., Vuleta, G.,& Savić, S.. (2016). Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies. in Tenside Surfactants Detergents
Carl Hanser Verlag, Munich., 53(3), 214-226.
https://doi.org/10.3139/113.110426

Filipović M, Lukić M, Krstonosić V, Đorđević S, Pantelić I, Gledović A, Vuleta G, Savić S. Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies. in Tenside Surfactants Detergents. 2016;53(3):214-226.
doi:10.3139/113.110426 .

Filipović, Mila, Lukić, Milica, Krstonosić, Veljko, Đorđević, Sanela, Pantelić, Ivana, Gledović, Ana, Vuleta, Gordana, Savić, Snežana, "Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies" in Tenside Surfactants Detergents, 53, no. 3 (2016):214-226,
https://doi.org/10.3139/113.110426 . .

TY  - CONF
AU  - Milić, Jela
AU  - Radojković, Branko
AU  - Stojanović, Biljana
AU  - Drašković, Jasmina
AU  - Mirašević, Slavica
AU  - Đorđević, Sanela
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2734
PB  - Elsevier Science BV, Amsterdam
C3  - International Journal of Pharmaceutics
T1  - Investigation of omeprazole stability in oral suspensions for pediatric use prepared extemporaneously from omeprazole capsules
VL  - 511
IS  - 2
SP  - 1147
EP  - 1148
DO  - 10.1016/j.ijpharm.2016.06.107
ER  - 

@conference{
author = "Milić, Jela and Radojković, Branko and Stojanović, Biljana and Drašković, Jasmina and Mirašević, Slavica and Đorđević, Sanela",
year = "2016",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Investigation of omeprazole stability in oral suspensions for pediatric use prepared extemporaneously from omeprazole capsules",
volume = "511",
number = "2",
pages = "1147-1148",
doi = "10.1016/j.ijpharm.2016.06.107"
}

Milić, J., Radojković, B., Stojanović, B., Drašković, J., Mirašević, S.,& Đorđević, S.. (2016). Investigation of omeprazole stability in oral suspensions for pediatric use prepared extemporaneously from omeprazole capsules. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 511(2), 1147-1148.
https://doi.org/10.1016/j.ijpharm.2016.06.107

Milić J, Radojković B, Stojanović B, Drašković J, Mirašević S, Đorđević S. Investigation of omeprazole stability in oral suspensions for pediatric use prepared extemporaneously from omeprazole capsules. in International Journal of Pharmaceutics. 2016;511(2):1147-1148.
doi:10.1016/j.ijpharm.2016.06.107 .

Milić, Jela, Radojković, Branko, Stojanović, Biljana, Drašković, Jasmina, Mirašević, Slavica, Đorđević, Sanela, "Investigation of omeprazole stability in oral suspensions for pediatric use prepared extemporaneously from omeprazole capsules" in International Journal of Pharmaceutics, 511, no. 2 (2016):1147-1148,
https://doi.org/10.1016/j.ijpharm.2016.06.107 . .

TY  - JOUR
AU  - Đorđević, Sanela
AU  - Cekić, Nebojša
AU  - Savić, Miroslav
AU  - Isailović, Tanja
AU  - Ranđelović, Danijela
AU  - Marković, Bojan
AU  - Savić, Saša R.
AU  - Timić-Stamenić, Tamara
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2346
AB  - This paper describes design and evaluation of parenteral lecithin-based nanoemulsions intended for brain delivery of risperidone, a poorly water-soluble psychopharmacological drug. The nanoemulsions were prepared through cold/hot high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, morphology, drug-vehicle interactions, and physical stability. To estimate the simultaneous influence of nanoemulsion formulation and preparation parameters-co-emulsifier type, aqueous phase type, homogenization temperature-on the critical quality attributes of developed nanoemulsions, a general factorial experimental design was applied. From the established design space and stability data, promising risperidone-loaded nanoemulsions (mean size about 160 nm, size distribution  lt 0.15, zeta potential around -50 mV), containing sodium oleate in the aqueous phase and polysorbate 80, poloxamer 188 or Solutol (R) HS15 as co-emulsifier, were produced by hot homogenization and their ability to improve risperidone delivery to the brain was assessed in rats. Pharmacokinetic study demonstrated erratic brain profiles of risperidone following intraperitoneal administration in selected nanoemulsions, most probably due to their different droplet surface properties (different composition of the stabilizing layer). Namely, polysorbate 80-costabilized nanoemulsion showed increased (1.4-7.4-fold higher) risperidone brain availability compared to other nanoemulsions and drug solution, suggesting this nanoemulsion as a promising carrier worth exploring further for brain targeting.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation
VL  - 493
IS  - 1-2
SP  - 40
EP  - 54
DO  - 10.1016/j.ijpharm.2015.07.007
ER  - 

@article{
author = "Đorđević, Sanela and Cekić, Nebojša and Savić, Miroslav and Isailović, Tanja and Ranđelović, Danijela and Marković, Bojan and Savić, Saša R. and Timić-Stamenić, Tamara and Daniels, Rolf and Savić, Snežana",
year = "2015",
abstract = "This paper describes design and evaluation of parenteral lecithin-based nanoemulsions intended for brain delivery of risperidone, a poorly water-soluble psychopharmacological drug. The nanoemulsions were prepared through cold/hot high pressure homogenization and characterized regarding droplet size, polydispersity, surface charge, morphology, drug-vehicle interactions, and physical stability. To estimate the simultaneous influence of nanoemulsion formulation and preparation parameters-co-emulsifier type, aqueous phase type, homogenization temperature-on the critical quality attributes of developed nanoemulsions, a general factorial experimental design was applied. From the established design space and stability data, promising risperidone-loaded nanoemulsions (mean size about 160 nm, size distribution  lt 0.15, zeta potential around -50 mV), containing sodium oleate in the aqueous phase and polysorbate 80, poloxamer 188 or Solutol (R) HS15 as co-emulsifier, were produced by hot homogenization and their ability to improve risperidone delivery to the brain was assessed in rats. Pharmacokinetic study demonstrated erratic brain profiles of risperidone following intraperitoneal administration in selected nanoemulsions, most probably due to their different droplet surface properties (different composition of the stabilizing layer). Namely, polysorbate 80-costabilized nanoemulsion showed increased (1.4-7.4-fold higher) risperidone brain availability compared to other nanoemulsions and drug solution, suggesting this nanoemulsion as a promising carrier worth exploring further for brain targeting.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation",
volume = "493",
number = "1-2",
pages = "40-54",
doi = "10.1016/j.ijpharm.2015.07.007"
}

Đorđević, S., Cekić, N., Savić, M., Isailović, T., Ranđelović, D., Marković, B., Savić, S. R., Timić-Stamenić, T., Daniels, R.,& Savić, S.. (2015). Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 493(1-2), 40-54.
https://doi.org/10.1016/j.ijpharm.2015.07.007

Đorđević S, Cekić N, Savić M, Isailović T, Ranđelović D, Marković B, Savić SR, Timić-Stamenić T, Daniels R, Savić S. Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation. in International Journal of Pharmaceutics. 2015;493(1-2):40-54.
doi:10.1016/j.ijpharm.2015.07.007 .

Đorđević, Sanela, Cekić, Nebojša, Savić, Miroslav, Isailović, Tanja, Ranđelović, Danijela, Marković, Bojan, Savić, Saša R., Timić-Stamenić, Tamara, Daniels, Rolf, Savić, Snežana, "Parenteral nanoemulsions as promising carriers for brain delivery of risperidone: Design, characterization and in vivo pharmacokinetic evaluation" in International Journal of Pharmaceutics, 493, no. 1-2 (2015):40-54,
https://doi.org/10.1016/j.ijpharm.2015.07.007 . .

TY  - JOUR
AU  - Cekić, Nebojša
AU  - Đorđević, Sanela
AU  - Savić, Saša R.
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2351
AB  - Using the experimental design methodology, we have developed and characterized nanoemulsions for a parenteral delivery using diazepam as a model drug. The formulations containing 20 or 30% (w/w) of medium chain triglycerides or the mixture of medium chain triglycerides and soybean oil as the oil phase, soybean lecithin and polysorbate 80 as emulsifiers, and a phosphate buffer solution as the aqueous phase were prepared by cold high pressure homogenization. The obtained nanoemulsions were evaluated in terms of droplet size, size distribution, surface charge, drug-vehicle interactions and physical stability. To evaluate the effects of the oil phase type, oil content and drug presence, as well as their interactions on critical quality attributes of nanoemulsions, a three-factor two-level full factorial design was applied. After the preparation, all nanoemulsions revealed small spherical droplets in the range 170-210 nm, with the narrow droplet size distribution ( lt  0.15) and the surface charge about -60 mV. The experimental design results indicated that not only factors alone (oil type, oil content, presence of drug), but their interactions also had a significant effect on the nanoemulsion droplet size, polydispersity index, and zeta potential. During two months of storage at 25°C, all nanoemulsions formulated with the medium chain triglycerides-soybean oil mixture (4:1, w/w) remained physically stable, without considerable changes in monitored parameters. Physicochemical characteristics and stability of these nanoemulsions demonstrated their suitability for parenteral drug delivery.
AB  - Cilj ovog rada bio je da se primenom metodologije eksperimentalnog dizajna razviju parenteralne nanoemulzije sa diazepamom kao model lekovitom supstancom i da se sprovede njihova sveobuhvatna fizičkohemijska karakterizacija. Metodom homogenizacije pod visokim pritiskom na sobnoj temperaturi izrađene su placebo i nanoemulzije sa lekom, stabilizovane smešom lecitina i polisorbata 80, variranjem udela i vrste uljane faze - 20 i 30% (m/m) triglicerida srednje dužine lanca ili smeše triglicerida srednje dužine lanca i sojinog ulja u odnosu 4:1. Dobijene nanoemulzije okarakterisane su u pogledu veličine i raspodele veličina kapi, površinskog naelektrisanja, interakcija lek-nosač i fizičke stabilnosti. U cilju procene istovremenog uticaja vrste uljane faze, udela ulja i prisustva leka, kao i njihovih interakcija, na kritične atribute kvaliteta nanoemulzija, primenjen je pun faktorski dizajn sa tri faktora na dva nivoa. Nakon izrade, sve formulacije nanoemulzija imale su malu veličinu kapi u opsegu 170-210 nm, sa veoma uskom raspodelom veličina (ispod 0,15) i površinskim naelektrisanjem oko -60 mV. Rezultati eksperimentalnog dizajna pokazali su da ne samo pojedinačni faktori (vrsta ulja, koncentracija ulja, prisustvo leka), nego i njihove interakcije, značajno utiču na veličinu kapi, indeks polidisperznosti i zeta potencijal ispitivanih nanoemulzija. Tokom 2 meseca čuvanja na 25°C, sve nanoemulzije formulisane sa smešom triglicerida srednje dužine lanca i sojinog ulja kao uljanom fazom bile su fizički stabilne, bez značajnih promena u praćenim parametrima. Fizičkohemijske karakteristike i stabilnost navedenih nanoemulzija ukazuju da one mogu biti potencijalni nosači za parenteralnu isporuku lekovitih supstanci.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Advanced Technologies
T1  - A full factorial design in the formulation of diazepam parenteral nanoemulsions: Physicochemical characterization and stability evaluation
T1  - Pun faktorski dizajn u formulaciji parenteralnih nanoemulzija sa diazepamom - fizičkohemijska karakterizacija i procena stabilnosti
VL  - 4
IS  - 1
SP  - 69
EP  - 77
DO  - 10.5937/savteh1501069C
ER  - 

@article{
author = "Cekić, Nebojša and Đorđević, Sanela and Savić, Saša R. and Savić, Snežana",
year = "2015",
abstract = "Using the experimental design methodology, we have developed and characterized nanoemulsions for a parenteral delivery using diazepam as a model drug. The formulations containing 20 or 30% (w/w) of medium chain triglycerides or the mixture of medium chain triglycerides and soybean oil as the oil phase, soybean lecithin and polysorbate 80 as emulsifiers, and a phosphate buffer solution as the aqueous phase were prepared by cold high pressure homogenization. The obtained nanoemulsions were evaluated in terms of droplet size, size distribution, surface charge, drug-vehicle interactions and physical stability. To evaluate the effects of the oil phase type, oil content and drug presence, as well as their interactions on critical quality attributes of nanoemulsions, a three-factor two-level full factorial design was applied. After the preparation, all nanoemulsions revealed small spherical droplets in the range 170-210 nm, with the narrow droplet size distribution ( lt  0.15) and the surface charge about -60 mV. The experimental design results indicated that not only factors alone (oil type, oil content, presence of drug), but their interactions also had a significant effect on the nanoemulsion droplet size, polydispersity index, and zeta potential. During two months of storage at 25°C, all nanoemulsions formulated with the medium chain triglycerides-soybean oil mixture (4:1, w/w) remained physically stable, without considerable changes in monitored parameters. Physicochemical characteristics and stability of these nanoemulsions demonstrated their suitability for parenteral drug delivery., Cilj ovog rada bio je da se primenom metodologije eksperimentalnog dizajna razviju parenteralne nanoemulzije sa diazepamom kao model lekovitom supstancom i da se sprovede njihova sveobuhvatna fizičkohemijska karakterizacija. Metodom homogenizacije pod visokim pritiskom na sobnoj temperaturi izrađene su placebo i nanoemulzije sa lekom, stabilizovane smešom lecitina i polisorbata 80, variranjem udela i vrste uljane faze - 20 i 30% (m/m) triglicerida srednje dužine lanca ili smeše triglicerida srednje dužine lanca i sojinog ulja u odnosu 4:1. Dobijene nanoemulzije okarakterisane su u pogledu veličine i raspodele veličina kapi, površinskog naelektrisanja, interakcija lek-nosač i fizičke stabilnosti. U cilju procene istovremenog uticaja vrste uljane faze, udela ulja i prisustva leka, kao i njihovih interakcija, na kritične atribute kvaliteta nanoemulzija, primenjen je pun faktorski dizajn sa tri faktora na dva nivoa. Nakon izrade, sve formulacije nanoemulzija imale su malu veličinu kapi u opsegu 170-210 nm, sa veoma uskom raspodelom veličina (ispod 0,15) i površinskim naelektrisanjem oko -60 mV. Rezultati eksperimentalnog dizajna pokazali su da ne samo pojedinačni faktori (vrsta ulja, koncentracija ulja, prisustvo leka), nego i njihove interakcije, značajno utiču na veličinu kapi, indeks polidisperznosti i zeta potencijal ispitivanih nanoemulzija. Tokom 2 meseca čuvanja na 25°C, sve nanoemulzije formulisane sa smešom triglicerida srednje dužine lanca i sojinog ulja kao uljanom fazom bile su fizički stabilne, bez značajnih promena u praćenim parametrima. Fizičkohemijske karakteristike i stabilnost navedenih nanoemulzija ukazuju da one mogu biti potencijalni nosači za parenteralnu isporuku lekovitih supstanci.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Advanced Technologies",
title = "A full factorial design in the formulation of diazepam parenteral nanoemulsions: Physicochemical characterization and stability evaluation, Pun faktorski dizajn u formulaciji parenteralnih nanoemulzija sa diazepamom - fizičkohemijska karakterizacija i procena stabilnosti",
volume = "4",
number = "1",
pages = "69-77",
doi = "10.5937/savteh1501069C"
}

Cekić, N., Đorđević, S., Savić, S. R.,& Savić, S.. (2015). A full factorial design in the formulation of diazepam parenteral nanoemulsions: Physicochemical characterization and stability evaluation. in Advanced Technologies
Univerzitet u Nišu - Tehnološki fakultet, Leskovac., 4(1), 69-77.
https://doi.org/10.5937/savteh1501069C

Cekić N, Đorđević S, Savić SR, Savić S. A full factorial design in the formulation of diazepam parenteral nanoemulsions: Physicochemical characterization and stability evaluation. in Advanced Technologies. 2015;4(1):69-77.
doi:10.5937/savteh1501069C .

Cekić, Nebojša, Đorđević, Sanela, Savić, Saša R., Savić, Snežana, "A full factorial design in the formulation of diazepam parenteral nanoemulsions: Physicochemical characterization and stability evaluation" in Advanced Technologies, 4, no. 1 (2015):69-77,
https://doi.org/10.5937/savteh1501069C . .

TY  - JOUR
AU  - Đorđević, Sanela
AU  - Cekić, Nebojša
AU  - Isailović, Tanja
AU  - Milić, Jela
AU  - Vuleta, Gordana
AU  - Lazić, Miodrag
AU  - Savić, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2064
AB  - The aim of the present study was to prepare oil-in-water nanoemulsions stabilized with a novel natural alkyl polyglucoside surfactant and to compare them with corresponding lecithin/polysorbate 80-based nanoemulsions in terms of physicochemical properties and physical stability. Nanoemulsions were prepared by high pressure homogenization, using 20, 30 and 40 mass% medium chain triglyceride as oil phase, and 4, 6 and 8 mass% lecithin/polysorbate 80 mixture (1/1) or caprylyl/capryl glucoside as emulsifiers. The effects of emulsifier type, emulsifier concentration and oil content were investigated with respect to changes in particle size, particle size distribution, surface charge and physical stability. The influence of production parameters (number of homogenization cycles, type of homogenization process, homogenization pressure) on particle size was also investigated. Analysis was performed by photon correlation spectroscopy, laser diffraction, zeta potential, pH and electrical conductivity measurements. All the produced formulations revealed a small droplet size ranging from 147 to 228 nm and a very narrow size distribution (polydispersity index range 0.072-0.124). Zeta potentials were found to be about -20 and -50 mV for nanoemulsions stabilized with lecithin/polysorbate 80 and caprylyl/capryl glucoside, respectively. The results obtained from the stability studies (6 months at 25 °C and 1 month at 40 °C) indicated that nanoemulsion stability was influenced by their composition. The results also suggested the most appropriate production parameters: 9 homogenization cycles, homogenization pressure of 500 bar and discontinuous process of homogenization.
AB  - Cilj ovog istraživanja bio je da se homogenizacijom pod visokim pritiskom izrade nanoemulzije stabilizovane alkil poliglukozidnim (APG) emulgatorom i da se njihove karakteristike i fizička stabilnost uporede sa nanoemulzijama stabilizovanim standardnom kombinacijom lecitina (L) i polisorbata 80 (P80). U istraživanju je praćen uticaj parametara formulacije, i to vrste i koncentracije emulgatora (smeša L/P80 vs. APG, u koncentraciji od 4, 6 i 8%) i koncentracije uljane faze (20, 30 i 40% trigliceridi srednje dužine lanca) na veličinu i raspodelu veličina kapi, zeta potencijal (ζ) i fizičku stabilnost nanoemulzija. Takođe je praćen uticaj procesnih parametara (postupak, pritisak i broj ciklusa homogenizacije) na veličinu kapi i indeks polidisperznosti (PdI), u cilju identifikovanja optimalnih uslova za izradu nanoemulzija. Dobijeni rezultati pokazuju da postoji uticaj sastava formulacije na karakteristike (L/P80 nanoemulzije: veličina kapi 147-156 nm, ζ oko -20 mV; APG nanoemulzije: veličina kapi 165-228 nm, ζ oko -50 mV) i fizičku stabilnost ispitivanih nanoemulzija, i da se diskontinualnim postupkom homogenizacije (9 ciklusa, 500 bar) mogu dobiti nanoemulzije optimalnih svojstava (veličina kapi, PdI).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Nanoemulsions produced by varying the type of emulsifier and oil content: Effect of formulation and process parameters on the characteristics and physical stability
T1  - Nanoemulzije dobijene variranjem tipa emulgatora i udela masne faze - uticaj formulacije i procesnih parametara na karakteristike i fizičku stabilnost
VL  - 67
IS  - 5
SP  - 795
EP  - 809
DO  - 10.2298/HEMIND120905005D
ER  - 

@article{
author = "Đorđević, Sanela and Cekić, Nebojša and Isailović, Tanja and Milić, Jela and Vuleta, Gordana and Lazić, Miodrag and Savić, Snežana",
year = "2013",
abstract = "The aim of the present study was to prepare oil-in-water nanoemulsions stabilized with a novel natural alkyl polyglucoside surfactant and to compare them with corresponding lecithin/polysorbate 80-based nanoemulsions in terms of physicochemical properties and physical stability. Nanoemulsions were prepared by high pressure homogenization, using 20, 30 and 40 mass% medium chain triglyceride as oil phase, and 4, 6 and 8 mass% lecithin/polysorbate 80 mixture (1/1) or caprylyl/capryl glucoside as emulsifiers. The effects of emulsifier type, emulsifier concentration and oil content were investigated with respect to changes in particle size, particle size distribution, surface charge and physical stability. The influence of production parameters (number of homogenization cycles, type of homogenization process, homogenization pressure) on particle size was also investigated. Analysis was performed by photon correlation spectroscopy, laser diffraction, zeta potential, pH and electrical conductivity measurements. All the produced formulations revealed a small droplet size ranging from 147 to 228 nm and a very narrow size distribution (polydispersity index range 0.072-0.124). Zeta potentials were found to be about -20 and -50 mV for nanoemulsions stabilized with lecithin/polysorbate 80 and caprylyl/capryl glucoside, respectively. The results obtained from the stability studies (6 months at 25 °C and 1 month at 40 °C) indicated that nanoemulsion stability was influenced by their composition. The results also suggested the most appropriate production parameters: 9 homogenization cycles, homogenization pressure of 500 bar and discontinuous process of homogenization., Cilj ovog istraživanja bio je da se homogenizacijom pod visokim pritiskom izrade nanoemulzije stabilizovane alkil poliglukozidnim (APG) emulgatorom i da se njihove karakteristike i fizička stabilnost uporede sa nanoemulzijama stabilizovanim standardnom kombinacijom lecitina (L) i polisorbata 80 (P80). U istraživanju je praćen uticaj parametara formulacije, i to vrste i koncentracije emulgatora (smeša L/P80 vs. APG, u koncentraciji od 4, 6 i 8%) i koncentracije uljane faze (20, 30 i 40% trigliceridi srednje dužine lanca) na veličinu i raspodelu veličina kapi, zeta potencijal (ζ) i fizičku stabilnost nanoemulzija. Takođe je praćen uticaj procesnih parametara (postupak, pritisak i broj ciklusa homogenizacije) na veličinu kapi i indeks polidisperznosti (PdI), u cilju identifikovanja optimalnih uslova za izradu nanoemulzija. Dobijeni rezultati pokazuju da postoji uticaj sastava formulacije na karakteristike (L/P80 nanoemulzije: veličina kapi 147-156 nm, ζ oko -20 mV; APG nanoemulzije: veličina kapi 165-228 nm, ζ oko -50 mV) i fizičku stabilnost ispitivanih nanoemulzija, i da se diskontinualnim postupkom homogenizacije (9 ciklusa, 500 bar) mogu dobiti nanoemulzije optimalnih svojstava (veličina kapi, PdI).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Nanoemulsions produced by varying the type of emulsifier and oil content: Effect of formulation and process parameters on the characteristics and physical stability, Nanoemulzije dobijene variranjem tipa emulgatora i udela masne faze - uticaj formulacije i procesnih parametara na karakteristike i fizičku stabilnost",
volume = "67",
number = "5",
pages = "795-809",
doi = "10.2298/HEMIND120905005D"
}

Đorđević, S., Cekić, N., Isailović, T., Milić, J., Vuleta, G., Lazić, M.,& Savić, S.. (2013). Nanoemulsions produced by varying the type of emulsifier and oil content: Effect of formulation and process parameters on the characteristics and physical stability. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 67(5), 795-809.
https://doi.org/10.2298/HEMIND120905005D

Đorđević S, Cekić N, Isailović T, Milić J, Vuleta G, Lazić M, Savić S. Nanoemulsions produced by varying the type of emulsifier and oil content: Effect of formulation and process parameters on the characteristics and physical stability. in Hemijska industrija. 2013;67(5):795-809.
doi:10.2298/HEMIND120905005D .

Đorđević, Sanela, Cekić, Nebojša, Isailović, Tanja, Milić, Jela, Vuleta, Gordana, Lazić, Miodrag, Savić, Snežana, "Nanoemulsions produced by varying the type of emulsifier and oil content: Effect of formulation and process parameters on the characteristics and physical stability" in Hemijska industrija, 67, no. 5 (2013):795-809,
https://doi.org/10.2298/HEMIND120905005D . .

TY  - JOUR
AU  - Đorđević, Sanela
AU  - Radulović, Tamara
AU  - Cekić, Nebojša
AU  - Ranđelović, Danijela
AU  - Savić, Miroslav
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Savić, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1873
AB  - With the aid of experimental design, we developed and characterized nanoemulsions for parenteral drug delivery. Formulations containing a mixture of medium-chain triglycerides and soybean oil as oil phase, lecithin (soybean/egg) and polysorbate 80 as emulsifiers, and 0.1M phosphate buffer solution (pH 8) as aqueous phase were prepared by cold high-pressure homogenization. To study the effects of the oil content, lecithin type, and the presence of diazepam as a model drug and their interactions on physicochemical characteristics of nanoemulsions, a three factor two-level full factorial design was applied. The nanoemulsions were evaluated concerning droplet size and size distribution, surface charge, viscosity, morphology, drug-excipient interactions, and physical stability. The characterization revealed the small spherical droplets in the range 195-220nm with polydispersity index below 0.15 and zeta potential between -30 and -60mV. Interactions among the investigated factors, rather than factors alone, were shown to more profoundly affect nanoemulsion characteristics. In vivo pharmacokinetic study of selected diazepam nanoemulsions with different oil content (20%, 30%, and 40%, w/w) demonstrated fast and intense initial distribution into rat brain of diazepam from nanoemulsions with 20% and 30% (w/w) oil content, suggesting their applicability in urgent situations.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Pharmaceutical Sciences
T1  - Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances
VL  - 102
IS  - 11
SP  - 4159
EP  - 4172
DO  - 10.1002/jps.23734
ER  - 

@article{
author = "Đorđević, Sanela and Radulović, Tamara and Cekić, Nebojša and Ranđelović, Danijela and Savić, Miroslav and Krajišnik, Danina and Milić, Jela and Savić, Snežana",
year = "2013",
abstract = "With the aid of experimental design, we developed and characterized nanoemulsions for parenteral drug delivery. Formulations containing a mixture of medium-chain triglycerides and soybean oil as oil phase, lecithin (soybean/egg) and polysorbate 80 as emulsifiers, and 0.1M phosphate buffer solution (pH 8) as aqueous phase were prepared by cold high-pressure homogenization. To study the effects of the oil content, lecithin type, and the presence of diazepam as a model drug and their interactions on physicochemical characteristics of nanoemulsions, a three factor two-level full factorial design was applied. The nanoemulsions were evaluated concerning droplet size and size distribution, surface charge, viscosity, morphology, drug-excipient interactions, and physical stability. The characterization revealed the small spherical droplets in the range 195-220nm with polydispersity index below 0.15 and zeta potential between -30 and -60mV. Interactions among the investigated factors, rather than factors alone, were shown to more profoundly affect nanoemulsion characteristics. In vivo pharmacokinetic study of selected diazepam nanoemulsions with different oil content (20%, 30%, and 40%, w/w) demonstrated fast and intense initial distribution into rat brain of diazepam from nanoemulsions with 20% and 30% (w/w) oil content, suggesting their applicability in urgent situations.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Pharmaceutical Sciences",
title = "Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances",
volume = "102",
number = "11",
pages = "4159-4172",
doi = "10.1002/jps.23734"
}

Đorđević, S., Radulović, T., Cekić, N., Ranđelović, D., Savić, M., Krajišnik, D., Milić, J.,& Savić, S.. (2013). Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances. in Journal of Pharmaceutical Sciences
Wiley-Blackwell, Hoboken., 102(11), 4159-4172.
https://doi.org/10.1002/jps.23734

Đorđević S, Radulović T, Cekić N, Ranđelović D, Savić M, Krajišnik D, Milić J, Savić S. Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances. in Journal of Pharmaceutical Sciences. 2013;102(11):4159-4172.
doi:10.1002/jps.23734 .

Đorđević, Sanela, Radulović, Tamara, Cekić, Nebojša, Ranđelović, Danijela, Savić, Miroslav, Krajišnik, Danina, Milić, Jela, Savić, Snežana, "Experimental Design in Formulation of Diazepam Nanoemulsions: Physicochemical and Pharmacokinetic Performances" in Journal of Pharmaceutical Sciences, 102, no. 11 (2013):4159-4172,
https://doi.org/10.1002/jps.23734 . .