@conference{
author = "Dobričić, Vladimir and Savić, Jelena and Tomašič, Tihomir and Zidar, Nace and Peterlin Mašič, Lucija and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2019",
abstract = "In this study, lipophilicity of twenty-three DNA gyrase and topoisomerase IV ATPase inhibitors was estimated at two
pH values (5.5 and 7.4) using reversed-phase high-performance liquid chromatography (RP-HPLC) [1,2]. Retention
behavior was tested on HP 1100 HPLC chromatograph, using column Zorbax Eclipse Plus C8 (150 X 4.6 mm, 5 µm
particle size). Mobile phase consisted of acetonitrile and phosphate buffer (pH was adjusted to 5.5 or 7.4). Each
compound was tested in four different ratios of acetonitrile and buffer (acetonitrile ranged from 20% to 65%). Column
temperature was 25 °C, flow rate 1 mL/min, injection volume 20 µL and detection was performed at 254 nm. For each
compound, capacity factor (k) was calculated and logk values were plotted against percentage of acetonitrile. Finally,
following chromatography parameters were calculated: logkw (y-axis intercept), a (slope) and ϕ0 (-logkw/a).
Derivatives with the highest lipophilicity were TEL-28 and NDL-20, whereas NZ97 had the lowest lipophilicity (at both
pH values, Figure 1). The majority of compounds possess similar or slightly different lipophilicities at both pH values,
but the highest differences were observed for TAZ-7, LMD-17 and NCH-4d, which could significantly affect their
biological properties (particularly gastrointestinal absorption, distribution and biological activity).",
publisher = "MuTaLig COST ACTION CA15135",
journal = "Third WG Meeting CA15135, Cost Action CA15135, Februar 23 - 24. 2019. Pariz, Francuska",
title = "RP-HPLC evaluation of lipophilicity of a series of dual DNA gyrase and topoisomerase IV inhibitors",
pages = "32-32",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5461"
}