TY  - CONF
AU  - Đoković, Nemanja
AU  - Ilić, Aleksandra
AU  - Čebzan, Alen
AU  - Radović, Branko
AU  - Ružić, Dušan
AU  - Đurić, Ana
AU  - Srdić-Rajić, Tatjana
AU  - Nikolić, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5001
AB  - Pancreatic ductal adenocarcinoma (PDAC) ranks among the most formidable and deadly
types of cancer. The emergence of chemoresistance in PDAC plays a significant role in the
unfavorable survival rates, making it imperative to swiftly develop new pharmaceutical
strategies to address this issue and improve treatment outcomes for PDAC (1). Considering
the numerous epigenetic changes observed in PDAC, the utilization of epigenetic drugs,
such as histone deacetylase (HDAC) inhibitors, holds a great promise as a transformative
approach, particularly when used in combination therapy settings (2).
In this study, we investigated the potential of utilizing drug sensitivity data and the basal
gene expression of pancreatic carcinoma cell lines to develop a bioinformatics screening
protocol for prediction of the combinatorial options available for HDAC inhibitors, including
sirtuin (SIRT) inhibitors. Experimental validation of the protocol performed on the two
pancreatic carcinoma cell lines (MIA PaCa-2 cells and PANC-1) confirmed the identified
synergisms between HDAC inhibitors and sphingosine 1-phosphate (S1P) receptor agonist
– fingolimod, or HDAC inhibitors and Rho-associated protein kinase (ROCK) inhibitor – RKI1447 (3).
Developed bioinformatics screening protocol for predictions of synergistic drug
combinations in PDAC identified several previously unreported interaction partners of
HDAC inhibitors. Predicted interaction partners of HDAC inhibitors including Aurora Kinase
A (AURKA) inhibitor, glutaminase (GLS) inhibitor and WEE1 kinase inhibitor were selected
for the design of novel classes of dual-acting HDAC inhibitors by the means of structure-based molecular modelling. Novel dual inhibitors (SIRT/AURKA, HDAC/GLS and HDAC/WEE1)
were designed relying on the known pharmacophoric features and molecular docking
models developed for each of the targets of interest. The docking scores of the designed
inhibitors revealed a notable affinity towards the specific targets. Additionally, when
combined with predictions of drug synergy, these designed molecules exhibit great
potential as promising structures for subsequent experimental evaluation.
PB  - International Association of Physical Chemists
C3  - 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
T1  - Towards the multitarget HDAC Inhibitors for the treatment of pancreatic carcinoma by joining the drug synergy predictions and the molecular modeling
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5001
ER  - 

@conference{
author = "Đoković, Nemanja and Ilić, Aleksandra and Čebzan, Alen and Radović, Branko and Ružić, Dušan and Đurić, Ana and Srdić-Rajić, Tatjana and Nikolić, Katarina",
year = "2023",
abstract = "Pancreatic ductal adenocarcinoma (PDAC) ranks among the most formidable and deadly
types of cancer. The emergence of chemoresistance in PDAC plays a significant role in the
unfavorable survival rates, making it imperative to swiftly develop new pharmaceutical
strategies to address this issue and improve treatment outcomes for PDAC (1). Considering
the numerous epigenetic changes observed in PDAC, the utilization of epigenetic drugs,
such as histone deacetylase (HDAC) inhibitors, holds a great promise as a transformative
approach, particularly when used in combination therapy settings (2).
In this study, we investigated the potential of utilizing drug sensitivity data and the basal
gene expression of pancreatic carcinoma cell lines to develop a bioinformatics screening
protocol for prediction of the combinatorial options available for HDAC inhibitors, including
sirtuin (SIRT) inhibitors. Experimental validation of the protocol performed on the two
pancreatic carcinoma cell lines (MIA PaCa-2 cells and PANC-1) confirmed the identified
synergisms between HDAC inhibitors and sphingosine 1-phosphate (S1P) receptor agonist
– fingolimod, or HDAC inhibitors and Rho-associated protein kinase (ROCK) inhibitor – RKI1447 (3).
Developed bioinformatics screening protocol for predictions of synergistic drug
combinations in PDAC identified several previously unreported interaction partners of
HDAC inhibitors. Predicted interaction partners of HDAC inhibitors including Aurora Kinase
A (AURKA) inhibitor, glutaminase (GLS) inhibitor and WEE1 kinase inhibitor were selected
for the design of novel classes of dual-acting HDAC inhibitors by the means of structure-based molecular modelling. Novel dual inhibitors (SIRT/AURKA, HDAC/GLS and HDAC/WEE1)
were designed relying on the known pharmacophoric features and molecular docking
models developed for each of the targets of interest. The docking scores of the designed
inhibitors revealed a notable affinity towards the specific targets. Additionally, when
combined with predictions of drug synergy, these designed molecules exhibit great
potential as promising structures for subsequent experimental evaluation.",
publisher = "International Association of Physical Chemists",
journal = "10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6",
title = "Towards the multitarget HDAC Inhibitors for the treatment of pancreatic carcinoma by joining the drug synergy predictions and the molecular modeling",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5001"
}

Đoković, N., Ilić, A., Čebzan, A., Radović, B., Ružić, D., Đurić, A., Srdić-Rajić, T.,& Nikolić, K.. (2023). Towards the multitarget HDAC Inhibitors for the treatment of pancreatic carcinoma by joining the drug synergy predictions and the molecular modeling. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
International Association of Physical Chemists., 47-47.
https://hdl.handle.net/21.15107/rcub_farfar_5001

Đoković N, Ilić A, Čebzan A, Radović B, Ružić D, Đurić A, Srdić-Rajić T, Nikolić K. Towards the multitarget HDAC Inhibitors for the treatment of pancreatic carcinoma by joining the drug synergy predictions and the molecular modeling. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6. 2023;:47-47.
https://hdl.handle.net/21.15107/rcub_farfar_5001 .

Đoković, Nemanja, Ilić, Aleksandra, Čebzan, Alen, Radović, Branko, Ružić, Dušan, Đurić, Ana, Srdić-Rajić, Tatjana, Nikolić, Katarina, "Towards the multitarget HDAC Inhibitors for the treatment of pancreatic carcinoma by joining the drug synergy predictions and the molecular modeling" in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 (2023):47-47,
https://hdl.handle.net/21.15107/rcub_farfar_5001 .

TY  - JOUR
AU  - Đoković, Nemanja
AU  - Đurić, Ana
AU  - Ružić, Dušan
AU  - Srdić-Rajić, Tatjana
AU  - Nikolić, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4472
AB  - Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies. Development of the chemoresistance in the PDAC is one of the key contributors to the poor survival outcomes and the major reason for urgent development of novel pharmacological approaches in a treatment of PDAC. Systematically tailored combination therapy holds the promise for advancing the treatment of PDAC. However, the number of possible combinations of pharmacological agents is too large to be explored experimentally. In respect to the many epigenetic alterations in PDAC, epigenetic drugs including histone deacetylase inhibitors (HDACi) could be seen as the game changers especially in combined therapy settings. In this work, we explored a possibility of using drug-sensitivity data together with the basal gene expression of pancreatic cell lines to predict combinatorial options available for HDACi. Developed bioinformatics screening protocol for predictions of synergistic drug combinations in PDAC identified the sphingolipid signaling pathway with associated downstream effectors as a promising novel targets for future development of multi-target therapeutics or combined therapy with HDACi. Through the experimental validation, we have characterized novel synergism between HDACi and a Rho-associated protein kinase (ROCK) inhibitor RKI-1447, and between HDACi and a sphingosine 1-phosphate (S1P) receptor agonist fingolimod.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Correlating Basal Gene Expression across Chemical Sensitivity Data to Screen for Novel Synergistic Interactors of HDAC Inhibitors in Pancreatic Carcinoma
VL  - 16
IS  - 2
DO  - 10.3390/ph16020294
ER  - 

@article{
author = "Đoković, Nemanja and Đurić, Ana and Ružić, Dušan and Srdić-Rajić, Tatjana and Nikolić, Katarina",
year = "2023",
abstract = "Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal malignancies. Development of the chemoresistance in the PDAC is one of the key contributors to the poor survival outcomes and the major reason for urgent development of novel pharmacological approaches in a treatment of PDAC. Systematically tailored combination therapy holds the promise for advancing the treatment of PDAC. However, the number of possible combinations of pharmacological agents is too large to be explored experimentally. In respect to the many epigenetic alterations in PDAC, epigenetic drugs including histone deacetylase inhibitors (HDACi) could be seen as the game changers especially in combined therapy settings. In this work, we explored a possibility of using drug-sensitivity data together with the basal gene expression of pancreatic cell lines to predict combinatorial options available for HDACi. Developed bioinformatics screening protocol for predictions of synergistic drug combinations in PDAC identified the sphingolipid signaling pathway with associated downstream effectors as a promising novel targets for future development of multi-target therapeutics or combined therapy with HDACi. Through the experimental validation, we have characterized novel synergism between HDACi and a Rho-associated protein kinase (ROCK) inhibitor RKI-1447, and between HDACi and a sphingosine 1-phosphate (S1P) receptor agonist fingolimod.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Correlating Basal Gene Expression across Chemical Sensitivity Data to Screen for Novel Synergistic Interactors of HDAC Inhibitors in Pancreatic Carcinoma",
volume = "16",
number = "2",
doi = "10.3390/ph16020294"
}

Đoković, N., Đurić, A., Ružić, D., Srdić-Rajić, T.,& Nikolić, K.. (2023). Correlating Basal Gene Expression across Chemical Sensitivity Data to Screen for Novel Synergistic Interactors of HDAC Inhibitors in Pancreatic Carcinoma. in Pharmaceuticals
MDPI., 16(2).
https://doi.org/10.3390/ph16020294

Đoković N, Đurić A, Ružić D, Srdić-Rajić T, Nikolić K. Correlating Basal Gene Expression across Chemical Sensitivity Data to Screen for Novel Synergistic Interactors of HDAC Inhibitors in Pancreatic Carcinoma. in Pharmaceuticals. 2023;16(2).
doi:10.3390/ph16020294 .

Đoković, Nemanja, Đurić, Ana, Ružić, Dušan, Srdić-Rajić, Tatjana, Nikolić, Katarina, "Correlating Basal Gene Expression across Chemical Sensitivity Data to Screen for Novel Synergistic Interactors of HDAC Inhibitors in Pancreatic Carcinoma" in Pharmaceuticals, 16, no. 2 (2023),
https://doi.org/10.3390/ph16020294 . .

TY  - JOUR
AU  - Gopčević, Kristina
AU  - Grujić, Slavica
AU  - Arsenijević, Jelena
AU  - Džamić, Ana
AU  - Veličković, Ivona
AU  - Izrael-Živković, Lidija
AU  - Medić, Ana
AU  - Mudrić, Jelena
AU  - Soković, Marina
AU  - Đurić, Ana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4342
AB  - The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.
PB  - Nature Research
T2  - Scientific Reports
T1  - Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction
VL  - 12
IS  - 1
DO  - 10.1038/s41598-022-25668-3
ER  - 

@article{
author = "Gopčević, Kristina and Grujić, Slavica and Arsenijević, Jelena and Džamić, Ana and Veličković, Ivona and Izrael-Živković, Lidija and Medić, Ana and Mudrić, Jelena and Soković, Marina and Đurić, Ana",
year = "2022",
abstract = "The aim of the study was to investigate the biological activity and chemical composition of Satureja kitaibelii Wierzb. ex Heuff. LC–PDA/MS analyses for the aqueous extracts (A1-stem, leaves and flowers, A2-leaves and flowers) and ethyl-acetate extracts (E1-stem, leaves and flowers, E2-leaves and flowers) obtained by ultrasound-assisted extraction enabled the identification of thirty-four compounds. Quantitative analysis revealed that the aqueous extract obtained from leaves and flowers was the richest in total phenolic acids (65.36 mg/g) and flavonoids (21.17 mg/g). The total polyphenol content was the highest in the aqueous extract obtained from leaves and flowers (274 ± 2.4 mg Gallic Acid equivalents/g). The best antioxidant activity was observed for the same extract using the DPPH (SC50 20 ± 10 µg/mL), ABTS (2.834 ± 0.02 mg Ascorbic Acid/g), FRAP (1.922 ± 0.03 mmol Fe2+/mg), and total reducing power tests (16.4 ± 1.0 mg Ascorbic Acid/g). Both ethyl acetate extracts were the most active against strains of Bacillus cereus and Micrococcus flavus (MIC 1.70–1.99 mg/mL and 1.99–3.41 mg/mL, respectively). They were more efficient against Aspergillus ochraceus (MFC 0.86 mg/mL) and towards HeLa cell lines. All the obtained results implied the good potential of the investigated extracts to be used as effective preservatives and functional ingredients in food products and dietary supplements.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction",
volume = "12",
number = "1",
doi = "10.1038/s41598-022-25668-3"
}

Gopčević, K., Grujić, S., Arsenijević, J., Džamić, A., Veličković, I., Izrael-Živković, L., Medić, A., Mudrić, J., Soković, M.,& Đurić, A.. (2022). Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports
Nature Research., 12(1).
https://doi.org/10.1038/s41598-022-25668-3

Gopčević K, Grujić S, Arsenijević J, Džamić A, Veličković I, Izrael-Živković L, Medić A, Mudrić J, Soković M, Đurić A. Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction. in Scientific Reports. 2022;12(1).
doi:10.1038/s41598-022-25668-3 .

Gopčević, Kristina, Grujić, Slavica, Arsenijević, Jelena, Džamić, Ana, Veličković, Ivona, Izrael-Živković, Lidija, Medić, Ana, Mudrić, Jelena, Soković, Marina, Đurić, Ana, "Bioactivity and phenolics profile of aqueous and ethyl acetate extracts of Satureja kitaibelii Wierzb. ex Heuff. obtained by ultrasound-assisted extraction" in Scientific Reports, 12, no. 1 (2022),
https://doi.org/10.1038/s41598-022-25668-3 . .

TY  - CONF
AU  - Ostojić, Marija
AU  - Đurić, Ana
AU  - Srdić-Rajić, Tatjana
AU  - Dobričić, Vladimir
AU  - Grahovac, Jelena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5477
AB  - Pancreatic ductal adenocarcinoma (PDAC) is the sixth leading cause of death worldwide and the fourth
in Europe with a 5-year survival rate. The common cause of treatment failure in PDAC patients is
multidrug resistance (MDR) due to the increased expression of plasma membrane efflux pumps that
limit the intracellular uptake and retention of numerous xeno- and endobiotics. As the 93.3% of
pancreatic carcinomas expressed P-glycoprotein (P-gp-MDR1/ABCB1) and 31% co-expressed multidrug
resistance protein 1 (MRP1/ABCC1) with MDR1 P-gp, the inhibition of these pumps may be the target
for novel anticancer drugs.
We used the FRED 3.2.0.2 software to predict the affinity of I1-imidazoline receptor ligand rilmenidine
within the binding site of P-gp-MDR1/ABCB1 and MRP1/ABCC1, and flow cytometry to evaluate the
effect of rilmenidine phosphate and rilmenidine fumarate on the efflux pumps in PDAC cells in vitro.
The results of the molecular docking studies indicate that rilmenidine has the binding affinity for both
P-gp-MDR1/ABCB1 and MRP1/ABCC1 efflux pumps. While, in vitro studies show that rilmenidine
fumarate has better potential to inhibit Calcein AM efflux than rilmenidine phosphate, and it did so in a
dose-dependent manner.
Our results indicate that rilmenidine has the affinity to bind to MDR efflux pumps and to inhibit their
activity. This potential of rilmenidine to overcome multidrug resistance in PDAC should be further
investigated in order to develop more effective PDAC therapy.
PB  - COST Action 17104 (STRATAGEM)
C3  - STRATAGEM CA17104, New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumors, 5th Annual Meeting, 29th June - 1st July 2022, Coimbra, Portugal
T1  - Rilmenidine binds to and inhibits the activity of MDR pumps in pancreatic ductal adenocarcinoma
SP  - 80
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5477
ER  - 

@conference{
author = "Ostojić, Marija and Đurić, Ana and Srdić-Rajić, Tatjana and Dobričić, Vladimir and Grahovac, Jelena",
year = "2022",
abstract = "Pancreatic ductal adenocarcinoma (PDAC) is the sixth leading cause of death worldwide and the fourth
in Europe with a 5-year survival rate. The common cause of treatment failure in PDAC patients is
multidrug resistance (MDR) due to the increased expression of plasma membrane efflux pumps that
limit the intracellular uptake and retention of numerous xeno- and endobiotics. As the 93.3% of
pancreatic carcinomas expressed P-glycoprotein (P-gp-MDR1/ABCB1) and 31% co-expressed multidrug
resistance protein 1 (MRP1/ABCC1) with MDR1 P-gp, the inhibition of these pumps may be the target
for novel anticancer drugs.
We used the FRED 3.2.0.2 software to predict the affinity of I1-imidazoline receptor ligand rilmenidine
within the binding site of P-gp-MDR1/ABCB1 and MRP1/ABCC1, and flow cytometry to evaluate the
effect of rilmenidine phosphate and rilmenidine fumarate on the efflux pumps in PDAC cells in vitro.
The results of the molecular docking studies indicate that rilmenidine has the binding affinity for both
P-gp-MDR1/ABCB1 and MRP1/ABCC1 efflux pumps. While, in vitro studies show that rilmenidine
fumarate has better potential to inhibit Calcein AM efflux than rilmenidine phosphate, and it did so in a
dose-dependent manner.
Our results indicate that rilmenidine has the affinity to bind to MDR efflux pumps and to inhibit their
activity. This potential of rilmenidine to overcome multidrug resistance in PDAC should be further
investigated in order to develop more effective PDAC therapy.",
publisher = "COST Action 17104 (STRATAGEM)",
journal = "STRATAGEM CA17104, New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumors, 5th Annual Meeting, 29th June - 1st July 2022, Coimbra, Portugal",
title = "Rilmenidine binds to and inhibits the activity of MDR pumps in pancreatic ductal adenocarcinoma",
pages = "80-80",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5477"
}

Ostojić, M., Đurić, A., Srdić-Rajić, T., Dobričić, V.,& Grahovac, J.. (2022). Rilmenidine binds to and inhibits the activity of MDR pumps in pancreatic ductal adenocarcinoma. in STRATAGEM CA17104, New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumors, 5th Annual Meeting, 29th June - 1st July 2022, Coimbra, Portugal
COST Action 17104 (STRATAGEM)., 80-80.
https://hdl.handle.net/21.15107/rcub_farfar_5477

Ostojić M, Đurić A, Srdić-Rajić T, Dobričić V, Grahovac J. Rilmenidine binds to and inhibits the activity of MDR pumps in pancreatic ductal adenocarcinoma. in STRATAGEM CA17104, New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumors, 5th Annual Meeting, 29th June - 1st July 2022, Coimbra, Portugal. 2022;:80-80.
https://hdl.handle.net/21.15107/rcub_farfar_5477 .

Ostojić, Marija, Đurić, Ana, Srdić-Rajić, Tatjana, Dobričić, Vladimir, Grahovac, Jelena, "Rilmenidine binds to and inhibits the activity of MDR pumps in pancreatic ductal adenocarcinoma" in STRATAGEM CA17104, New Diagnostic and Therapeutic Tools against Multidrug Resistant Tumors, 5th Annual Meeting, 29th June - 1st July 2022, Coimbra, Portugal (2022):80-80,
https://hdl.handle.net/21.15107/rcub_farfar_5477 .

TY  - JOUR
AU  - Ružić, Dušan
AU  - Ellinger, Bernhard
AU  - Đoković, Nemanja
AU  - Santibanez, Juan
AU  - Gul, Sheraz
AU  - Beljkaš, Milan
AU  - Đurić, Ana
AU  - Ganesan, Arasu
AU  - Pavić, Aleksandar
AU  - Srdić-Rajić, Tatjana
AU  - Petković, Miloš
AU  - Nikolić, Katarina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4368
AB  - Isoform-selective histone deacetylase (HDAC) inhibition is promoted as a rational strategy to develop safer anti-cancer drugs compared to non-selective HDAC inhibitors. Despite this presumed benefit, considerably more non-selective HDAC inhibitors have undergone clinical trials. In this report, we detail the design and discovery of potent HDAC inhibitors, with 1-benzhydryl piperazine as a surface recognition group, that differ in hydrocarbon linker. In vitro HDAC screening identified two selective HDAC6 inhibitors with nanomolar IC50 values, as well as two non-selective nanomolar HDAC inhibitors. Structure-based molecular modeling was employed to study the influence of linker chemistry of synthesized inhibitors on HDAC6 potency. The breast cancer cell lines (MDA-MB-231 and MCF-7) were used to evaluate compound-mediated in vitro anti-cancer, anti-migratory, and anti-invasive activities. Experiments on the zebrafish MDA-MB-231 xenograft model revealed that a novel non-selective HDAC inhibitor with a seven-carbon-atom linker exhibits potent anti-tumor, anti-metastatic, and anti-angiogenic effects when tested at low micromolar concentrations.
PB  - MDPI
T2  - Pharmaceutics
T1  - Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation
VL  - 14
IS  - 12
DO  - 10.3390/pharmaceutics14122600
ER  - 

@article{
author = "Ružić, Dušan and Ellinger, Bernhard and Đoković, Nemanja and Santibanez, Juan and Gul, Sheraz and Beljkaš, Milan and Đurić, Ana and Ganesan, Arasu and Pavić, Aleksandar and Srdić-Rajić, Tatjana and Petković, Miloš and Nikolić, Katarina",
year = "2022",
abstract = "Isoform-selective histone deacetylase (HDAC) inhibition is promoted as a rational strategy to develop safer anti-cancer drugs compared to non-selective HDAC inhibitors. Despite this presumed benefit, considerably more non-selective HDAC inhibitors have undergone clinical trials. In this report, we detail the design and discovery of potent HDAC inhibitors, with 1-benzhydryl piperazine as a surface recognition group, that differ in hydrocarbon linker. In vitro HDAC screening identified two selective HDAC6 inhibitors with nanomolar IC50 values, as well as two non-selective nanomolar HDAC inhibitors. Structure-based molecular modeling was employed to study the influence of linker chemistry of synthesized inhibitors on HDAC6 potency. The breast cancer cell lines (MDA-MB-231 and MCF-7) were used to evaluate compound-mediated in vitro anti-cancer, anti-migratory, and anti-invasive activities. Experiments on the zebrafish MDA-MB-231 xenograft model revealed that a novel non-selective HDAC inhibitor with a seven-carbon-atom linker exhibits potent anti-tumor, anti-metastatic, and anti-angiogenic effects when tested at low micromolar concentrations.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation",
volume = "14",
number = "12",
doi = "10.3390/pharmaceutics14122600"
}

Ružić, D., Ellinger, B., Đoković, N., Santibanez, J., Gul, S., Beljkaš, M., Đurić, A., Ganesan, A., Pavić, A., Srdić-Rajić, T., Petković, M.,& Nikolić, K.. (2022). Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation. in Pharmaceutics
MDPI., 14(12).
https://doi.org/10.3390/pharmaceutics14122600

Ružić D, Ellinger B, Đoković N, Santibanez J, Gul S, Beljkaš M, Đurić A, Ganesan A, Pavić A, Srdić-Rajić T, Petković M, Nikolić K. Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation. in Pharmaceutics. 2022;14(12).
doi:10.3390/pharmaceutics14122600 .

Ružić, Dušan, Ellinger, Bernhard, Đoković, Nemanja, Santibanez, Juan, Gul, Sheraz, Beljkaš, Milan, Đurić, Ana, Ganesan, Arasu, Pavić, Aleksandar, Srdić-Rajić, Tatjana, Petković, Miloš, Nikolić, Katarina, "Discovery of 1-Benzhydryl-Piperazine-Based HDAC Inhibitors with Anti-Breast Cancer Activity: Synthesis, Molecular Modeling, In Vitro and In Vivo Biological Evaluation" in Pharmaceutics, 14, no. 12 (2022),
https://doi.org/10.3390/pharmaceutics14122600 . .

TY  - JOUR
AU  - Pavlović, Miloš
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurić, Ana
AU  - Stanojević, Boban
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3956
AB  - Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy.
AB  - Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.
PB  - Belgrade: Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Moderate radioprotective role of zeolite in rats
T1  - Umerena radioprotektivna uloga zeolita kod pacova
VL  - 78
IS  - 7
SP  - 760
EP  - 768
DO  - 10.2298/VSP190702136P
ER  - 

@article{
author = "Pavlović, Miloš and Đukić, Mirjana and Vojvodić, Danilo and Ninković, Milica and Stevanović, Ivana and Đurić, Ana and Stanojević, Boban",
year = "2021",
abstract = "Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy., Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.",
publisher = "Belgrade: Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Moderate radioprotective role of zeolite in rats, Umerena radioprotektivna uloga zeolita kod pacova",
volume = "78",
number = "7",
pages = "760-768",
doi = "10.2298/VSP190702136P"
}

Pavlović, M., Đukić, M., Vojvodić, D., Ninković, M., Stevanović, I., Đurić, A.,& Stanojević, B.. (2021). Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled
Belgrade: Military Medical Academy, INI., 78(7), 760-768.
https://doi.org/10.2298/VSP190702136P

Pavlović M, Đukić M, Vojvodić D, Ninković M, Stevanović I, Đurić A, Stanojević B. Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled. 2021;78(7):760-768.
doi:10.2298/VSP190702136P .

Pavlović, Miloš, Đukić, Mirjana, Vojvodić, Danilo, Ninković, Milica, Stevanović, Ivana, Đurić, Ana, Stanojević, Boban, "Moderate radioprotective role of zeolite in rats" in Vojnosanitetski pregled, 78, no. 7 (2021):760-768,
https://doi.org/10.2298/VSP190702136P . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Fesatidou, Mara
AU  - Xenikakis, Iakovos
AU  - Geronikaki, Athina
AU  - Angelova, Violina T.
AU  - Savić, Vladimir
AU  - Pasić, Marta
AU  - Krilović, Branislav
AU  - Đukić, Dušan
AU  - Gobeljić, Borko
AU  - Pavlica, Marina
AU  - Đurić, Ana
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Saso, Luciano
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3154
AB  - The initial steps in preclinical drug developing research concern the synthesis of new compounds for specific therapeutic use which needs to be confirmed by in vitro and then in vivo testing. Nine thiazolidinone derivatives (numerically labeled 1-9) classified as follows: 1,3-thiazole-based compounds (1 and 2); 1,3,4-thiadiazole based compounds (3 and 4); substituted 5-benzylideno-2-adamantylthiazol[3,2-b] [1,2,4] triazol-6(5H) ones (5-8); and an ethylaminothiazole-based chalcone (9), were tested for antioxidant activity (AOA) by using three in vitro assays: DPPH (1,1-diphenyl-2-picrylhydrazyl scavenging capacity test); FRAP (ferric reducing antioxidant power test); and TBARS (thiobarbituric acid reactive substances test). Compounds 1-4 and 9 in particular are newly synthesized compounds. Also, traditional antioxidants Vitamins E and C and alpha-lipoic acid (alpha-LA) were tested. The results of DPPH testing: Vitamin C 94.35%, Vitamin E 2.99% and alpha-LA 1.57%; compounds: 4 33.98%; 2 18.73%; 1 15.62%; 5 6.59%; 3 4.99%; 6-9 demonstrated almost no AOA. The results of TBARS testing (% of LPO inhibition): Vitamin C 62.32%; Vitamin E 36.29%; alpha-LA 51.36%; compounds: 1 62.11%; 5 66.71%; 9 60.93%; 4, 6 and 7 demonstrated similar to 50%; 3 and 8 displayed similar to 38%; 2 23.51%. By FRAP method, Vitamins E and C showed equal AOA, similar to 100%, unlike alpha-LA (no AOA), and AOA of the tested compounds (expressed as a fraction of the AOA of Vitamin C) were: 2 and 4-75%; 8, 3 and 1-45%; 5-7 and 9-27%. Different red-ox reaction principles between these assays dictate different AOA outcomes for a single compound. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Phenyl-functionalized benzylidene, amino-carbonyl functional domains and chelating ligand properties of the thiazolidinone derivatives correlated with AOA.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole
VL  - 286
SP  - 119
EP  - 131
DO  - 10.1016/j.cbi.2018.03.013
ER  - 

@article{
author = "Đukić, Mirjana and Fesatidou, Mara and Xenikakis, Iakovos and Geronikaki, Athina and Angelova, Violina T. and Savić, Vladimir and Pasić, Marta and Krilović, Branislav and Đukić, Dušan and Gobeljić, Borko and Pavlica, Marina and Đurić, Ana and Stanojević, Ivan and Vojvodić, Danilo and Saso, Luciano",
year = "2018",
abstract = "The initial steps in preclinical drug developing research concern the synthesis of new compounds for specific therapeutic use which needs to be confirmed by in vitro and then in vivo testing. Nine thiazolidinone derivatives (numerically labeled 1-9) classified as follows: 1,3-thiazole-based compounds (1 and 2); 1,3,4-thiadiazole based compounds (3 and 4); substituted 5-benzylideno-2-adamantylthiazol[3,2-b] [1,2,4] triazol-6(5H) ones (5-8); and an ethylaminothiazole-based chalcone (9), were tested for antioxidant activity (AOA) by using three in vitro assays: DPPH (1,1-diphenyl-2-picrylhydrazyl scavenging capacity test); FRAP (ferric reducing antioxidant power test); and TBARS (thiobarbituric acid reactive substances test). Compounds 1-4 and 9 in particular are newly synthesized compounds. Also, traditional antioxidants Vitamins E and C and alpha-lipoic acid (alpha-LA) were tested. The results of DPPH testing: Vitamin C 94.35%, Vitamin E 2.99% and alpha-LA 1.57%; compounds: 4 33.98%; 2 18.73%; 1 15.62%; 5 6.59%; 3 4.99%; 6-9 demonstrated almost no AOA. The results of TBARS testing (% of LPO inhibition): Vitamin C 62.32%; Vitamin E 36.29%; alpha-LA 51.36%; compounds: 1 62.11%; 5 66.71%; 9 60.93%; 4, 6 and 7 demonstrated similar to 50%; 3 and 8 displayed similar to 38%; 2 23.51%. By FRAP method, Vitamins E and C showed equal AOA, similar to 100%, unlike alpha-LA (no AOA), and AOA of the tested compounds (expressed as a fraction of the AOA of Vitamin C) were: 2 and 4-75%; 8, 3 and 1-45%; 5-7 and 9-27%. Different red-ox reaction principles between these assays dictate different AOA outcomes for a single compound. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Phenyl-functionalized benzylidene, amino-carbonyl functional domains and chelating ligand properties of the thiazolidinone derivatives correlated with AOA.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole",
volume = "286",
pages = "119-131",
doi = "10.1016/j.cbi.2018.03.013"
}

Đukić, M., Fesatidou, M., Xenikakis, I., Geronikaki, A., Angelova, V. T., Savić, V., Pasić, M., Krilović, B., Đukić, D., Gobeljić, B., Pavlica, M., Đurić, A., Stanojević, I., Vojvodić, D.,& Saso, L.. (2018). In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 286, 119-131.
https://doi.org/10.1016/j.cbi.2018.03.013

Đukić M, Fesatidou M, Xenikakis I, Geronikaki A, Angelova VT, Savić V, Pasić M, Krilović B, Đukić D, Gobeljić B, Pavlica M, Đurić A, Stanojević I, Vojvodić D, Saso L. In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole. in Chemico-Biological Interactions. 2018;286:119-131.
doi:10.1016/j.cbi.2018.03.013 .

Đukić, Mirjana, Fesatidou, Mara, Xenikakis, Iakovos, Geronikaki, Athina, Angelova, Violina T., Savić, Vladimir, Pasić, Marta, Krilović, Branislav, Đukić, Dušan, Gobeljić, Borko, Pavlica, Marina, Đurić, Ana, Stanojević, Ivan, Vojvodić, Danilo, Saso, Luciano, "In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole" in Chemico-Biological Interactions, 286 (2018):119-131,
https://doi.org/10.1016/j.cbi.2018.03.013 . .

TY  - JOUR
AU  - Pantić Biševac, Jelena
AU  - Đukić, Mirjana
AU  - Stanojević, Ivan
AU  - Stevanović, Ivana
AU  - Mijušković, Željko
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Banović, Tatjana
AU  - Vojvodić, Danilo
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3206
AB  - Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Association between oxidative stress and melanoma progression
VL  - 37
IS  - 1
SP  - 12
EP  - 20
DO  - 10.1515/jomb-2017-0040
ER  - 

@article{
author = "Pantić Biševac, Jelena and Đukić, Mirjana and Stanojević, Ivan and Stevanović, Ivana and Mijušković, Željko and Đurić, Ana and Gobeljić, Borko and Banović, Tatjana and Vojvodić, Danilo",
year = "2018",
abstract = "Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Association between oxidative stress and melanoma progression",
volume = "37",
number = "1",
pages = "12-20",
doi = "10.1515/jomb-2017-0040"
}

Pantić Biševac, J., Đukić, M., Stanojević, I., Stevanović, I., Mijušković, Ž., Đurić, A., Gobeljić, B., Banović, T.,& Vojvodić, D.. (2018). Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 37(1), 12-20.
https://doi.org/10.1515/jomb-2017-0040

Pantić Biševac J, Đukić M, Stanojević I, Stevanović I, Mijušković Ž, Đurić A, Gobeljić B, Banović T, Vojvodić D. Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry. 2018;37(1):12-20.
doi:10.1515/jomb-2017-0040 .

Pantić Biševac, Jelena, Đukić, Mirjana, Stanojević, Ivan, Stevanović, Ivana, Mijušković, Željko, Đurić, Ana, Gobeljić, Borko, Banović, Tatjana, Vojvodić, Danilo, "Association between oxidative stress and melanoma progression" in Journal of Medical Biochemistry, 37, no. 1 (2018):12-20,
https://doi.org/10.1515/jomb-2017-0040 . .

TY  - THES
AU  - Đurić, Ana
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5449
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16836/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49498383
UR  - http://nardus.mpn.gov.rs/123456789/8967
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3386
AB  - Dosadašnja istraživanja mehanizama toksičnosti alkohola i kadmijuma (Cd) pri pojedinačnoj i udruženoj izloženosti eksprimantalnih životinja potvrđuju da oba agensa dovode do reproduktivne toksičnosti. Brojne epidemiološke studije su takođe ukazale da su pušenje i alkoholizam uzročnici poremećaja u spermatogenezi i neplodnosti kod muškaraca. S obzirom na činjenicu da je duvanski dim značajan izvor izloženosti Cd, kao i da su alkoholičari uglavnom i pušači, potrebno je utvrditi kako disulfiram (DSF), lek koji se koristi u averzivnoj terapiji alkoholizma, utiče na reproduktivnu toksičnost izazvanu pomenutim toksikantima. Cilj ove studije je bio da se ispita efekat DSF na narušenu homeostazu bioelemenata, redoks i androgeni status u testisima pacova prethodno izloženih alkoholu i/ili Cd kao i tokom koekspozicije sa Cd. Slobodna sulfhidrilna grupa (–SH) u hemijskoj strukturi metabolita DSF, dietilditiokarbamata (DDTC), ukazuje na redukcioni i helirajući potencijal DSF koji umnogome može da promeni metabolizam kako ispitivanih toksičnih agenasa (alkohola i Cd), tako i esencijalnih bioelemenata, kao i endogenih jedinjenja koja poseduju –SH grupe zbog mogućih reakcija tiolnog ukrštanja.Studija na životinjama (mužjaci pacova Wistar soja, n = 288) je dizajnirana sa ciljem da se imitiraju navike alkoholičara-pušača pre i nakon uvođenja DSF. Životinje su bile izložene ispitivanim supstancama subakutno (alkoholu i DSF), odnosno subhronično (Cd). Alkohol, DSF i maslinovo ulje (MU) kao rastvarač za DSF, su davani putem oralne sonde (per os), a Cd intraperitonelano (i.p.), svakodnevno u jednoj dozi. Životinje su slučajnim odabirom podeljene na kontrolne grupe i 7 eksperimentalnih grupa, od kojih je svaka grupa podeljena u 4 ili 6 podgrupa (n = 6) u odnosu na dužinu izloženosti svakoj od ispitivanih supstanci pojedinačno ili njihovoj kombinovanoj primeni. Podela eksperimentalnih životinja na grupe u odnosu na trajanje ekspozicije (1, 3, 10, 21 i 42 dana) je sledeća: tri kontrolne grupe (K1-42 , MU1-21, i K1-21/MU22-42); tri grupe koje su individualno bile izložene ispitivanim agensima (A1-21, Cd1-42 i DSF1-21); i četiri grupe koje su bile paralelno izložene ispitivanim agesnima (A1-21/Cd1-21, A1-21/DSF22-42, Cd1-42/DSF22-42 i A1-21/Cd1-42/DSF22-42)...
AB  - Previous animal studies on the mechanisms of toxicity after exposure or co-exposure to alcohol and cadmium (Cd) confirmed that both agents induce reproductive toxicity. Numerous epidemiological studies have also suggested that smoking and alcoholism cause disturbances in spermatogenesis and infertility in men. Due to the fact that tobacco smoke is an important source of Cd exposure, as well as alcoholics are usually smokers too, it is necessary to determine if disulfiram (DSF), a drug used in aversive therapy of alcoholism, affects the reproductive toxicity induced by the abovementioned toxicants. The aim of this study was to examine the effect of DSF on disturbed bioelements’ homeostasis, redox and androgen status in the testes of rats previously exposed to alcohol and / or Cd and during co-exposure with Cd. Free sulfhydryl group (-SH) in the chemical structure of the DSF’s metabolite, diethyldithiocarbamate (DDTC), indicates the reducing and chelating potential of DSF, which in many ways can change the metabolism of tested toxic agents (A and Cd), as well as essential bioelements, and endogenous compounds containing -SH groups due to possible reactions of thiol linking.The animal study (male Wistar rats, n = 288) was designed in order to imitate the conditions of alcoholics-smokers before and after the introduction of DSF. The animals were exposed to the tested substances subacute (alcohol and DSF) and subchronic (Cd). Alcohol, DSF, and olive oil (OL), as a solvent for DSF, were administered via oral gavage (per os), and Cd intraperitoneally, in a single daily dose. Animals were randomly divided into control groups and 7 experimental groups, each group was divided into 4 or 6 subgroups (n = 6) according to the duration of exposure to the tested substances alone or in combination. The division of the groups of the experimental animals with respect to the exposure time (1, 3, 10, 21 and 42 days) was as follows: three control groups (C1-42, OL1-21 and C1-21 / OL22-42); three groups which were independently exposed to the tested agents (A1-21, Cd1-42 and DSF1-21); and four groups that were subjected parallelly to the tested agents (A1-21 / Cd1-21, A1-21 / DSF22-42, Cd1-42 / DSF22-42 and A1-21 / Cd1-42 / DSF22-42)...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8967
ER  - 

@phdthesis{
author = "Đurić, Ana",
year = "2017",
abstract = "Dosadašnja istraživanja mehanizama toksičnosti alkohola i kadmijuma (Cd) pri pojedinačnoj i udruženoj izloženosti eksprimantalnih životinja potvrđuju da oba agensa dovode do reproduktivne toksičnosti. Brojne epidemiološke studije su takođe ukazale da su pušenje i alkoholizam uzročnici poremećaja u spermatogenezi i neplodnosti kod muškaraca. S obzirom na činjenicu da je duvanski dim značajan izvor izloženosti Cd, kao i da su alkoholičari uglavnom i pušači, potrebno je utvrditi kako disulfiram (DSF), lek koji se koristi u averzivnoj terapiji alkoholizma, utiče na reproduktivnu toksičnost izazvanu pomenutim toksikantima. Cilj ove studije je bio da se ispita efekat DSF na narušenu homeostazu bioelemenata, redoks i androgeni status u testisima pacova prethodno izloženih alkoholu i/ili Cd kao i tokom koekspozicije sa Cd. Slobodna sulfhidrilna grupa (–SH) u hemijskoj strukturi metabolita DSF, dietilditiokarbamata (DDTC), ukazuje na redukcioni i helirajući potencijal DSF koji umnogome može da promeni metabolizam kako ispitivanih toksičnih agenasa (alkohola i Cd), tako i esencijalnih bioelemenata, kao i endogenih jedinjenja koja poseduju –SH grupe zbog mogućih reakcija tiolnog ukrštanja.Studija na životinjama (mužjaci pacova Wistar soja, n = 288) je dizajnirana sa ciljem da se imitiraju navike alkoholičara-pušača pre i nakon uvođenja DSF. Životinje su bile izložene ispitivanim supstancama subakutno (alkoholu i DSF), odnosno subhronično (Cd). Alkohol, DSF i maslinovo ulje (MU) kao rastvarač za DSF, su davani putem oralne sonde (per os), a Cd intraperitonelano (i.p.), svakodnevno u jednoj dozi. Životinje su slučajnim odabirom podeljene na kontrolne grupe i 7 eksperimentalnih grupa, od kojih je svaka grupa podeljena u 4 ili 6 podgrupa (n = 6) u odnosu na dužinu izloženosti svakoj od ispitivanih supstanci pojedinačno ili njihovoj kombinovanoj primeni. Podela eksperimentalnih životinja na grupe u odnosu na trajanje ekspozicije (1, 3, 10, 21 i 42 dana) je sledeća: tri kontrolne grupe (K1-42 , MU1-21, i K1-21/MU22-42); tri grupe koje su individualno bile izložene ispitivanim agensima (A1-21, Cd1-42 i DSF1-21); i četiri grupe koje su bile paralelno izložene ispitivanim agesnima (A1-21/Cd1-21, A1-21/DSF22-42, Cd1-42/DSF22-42 i A1-21/Cd1-42/DSF22-42)..., Previous animal studies on the mechanisms of toxicity after exposure or co-exposure to alcohol and cadmium (Cd) confirmed that both agents induce reproductive toxicity. Numerous epidemiological studies have also suggested that smoking and alcoholism cause disturbances in spermatogenesis and infertility in men. Due to the fact that tobacco smoke is an important source of Cd exposure, as well as alcoholics are usually smokers too, it is necessary to determine if disulfiram (DSF), a drug used in aversive therapy of alcoholism, affects the reproductive toxicity induced by the abovementioned toxicants. The aim of this study was to examine the effect of DSF on disturbed bioelements’ homeostasis, redox and androgen status in the testes of rats previously exposed to alcohol and / or Cd and during co-exposure with Cd. Free sulfhydryl group (-SH) in the chemical structure of the DSF’s metabolite, diethyldithiocarbamate (DDTC), indicates the reducing and chelating potential of DSF, which in many ways can change the metabolism of tested toxic agents (A and Cd), as well as essential bioelements, and endogenous compounds containing -SH groups due to possible reactions of thiol linking.The animal study (male Wistar rats, n = 288) was designed in order to imitate the conditions of alcoholics-smokers before and after the introduction of DSF. The animals were exposed to the tested substances subacute (alcohol and DSF) and subchronic (Cd). Alcohol, DSF, and olive oil (OL), as a solvent for DSF, were administered via oral gavage (per os), and Cd intraperitoneally, in a single daily dose. Animals were randomly divided into control groups and 7 experimental groups, each group was divided into 4 or 6 subgroups (n = 6) according to the duration of exposure to the tested substances alone or in combination. The division of the groups of the experimental animals with respect to the exposure time (1, 3, 10, 21 and 42 days) was as follows: three control groups (C1-42, OL1-21 and C1-21 / OL22-42); three groups which were independently exposed to the tested agents (A1-21, Cd1-42 and DSF1-21); and four groups that were subjected parallelly to the tested agents (A1-21 / Cd1-21, A1-21 / DSF22-42, Cd1-42 / DSF22-42 and A1-21 / Cd1-42 / DSF22-42)...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8967"
}

Đurić, A.. (2017). Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_8967

Đurić A. Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova. in Универзитет у Београду. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8967 .

Đurić, Ana, "Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova" in Универзитет у Београду (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8967 .

TY  - JOUR
AU  - Đurić, Ana
AU  - Begić, Aida
AU  - Gobeljić, Borko
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Ninković, Milica
AU  - Prokić, Vera
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2969
AB  - The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes
VL  - 105
SP  - 44
EP  - 51
DO  - 10.1016/j.fct.2017.03.041
ER  - 

@article{
author = "Đurić, Ana and Begić, Aida and Gobeljić, Borko and Pantelić, Ana and Zebić, Goran and Stevanović, Ivana and Đurđević, Dragan and Ninković, Milica and Prokić, Vera and Stanojević, Ivan and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes",
volume = "105",
pages = "44-51",
doi = "10.1016/j.fct.2017.03.041"
}

Đurić, A., Begić, A., Gobeljić, B., Pantelić, A., Zebić, G., Stevanović, I., Đurđević, D., Ninković, M., Prokić, V., Stanojević, I., Vojvodić, D.,& Đukić, M.. (2017). Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 105, 44-51.
https://doi.org/10.1016/j.fct.2017.03.041

Đurić A, Begić A, Gobeljić B, Pantelić A, Zebić G, Stevanović I, Đurđević D, Ninković M, Prokić V, Stanojević I, Vojvodić D, Đukić M. Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology. 2017;105:44-51.
doi:10.1016/j.fct.2017.03.041 .

Đurić, Ana, Begić, Aida, Gobeljić, Borko, Pantelić, Ana, Zebić, Goran, Stevanović, Ivana, Đurđević, Dragan, Ninković, Milica, Prokić, Vera, Stanojević, Ivan, Vojvodić, Danilo, Đukić, Mirjana, "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes" in Food and Chemical Toxicology, 105 (2017):44-51,
https://doi.org/10.1016/j.fct.2017.03.041 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Pavlović, Miloš
AU  - Jelić, Katarina
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Dejanović, Bratislav
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Milosavljević, Petar
AU  - Đukić, Mirjana
AU  - Saso, Luciano
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2963
AB  - Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Enzyme Inhibition and Medicinal Chemistry
T1  - Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium
VL  - 32
IS  - 1
SP  - 478
EP  - 489
DO  - 10.1080/14756366.2016.1261132
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Pavlović, Miloš and Jelić, Katarina and Pantelić, Ana and Zebić, Goran and Dejanović, Bratislav and Stanojević, Ivan and Vojvodić, Danilo and Milosavljević, Petar and Đukić, Mirjana and Saso, Luciano",
year = "2017",
abstract = "Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
title = "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium",
volume = "32",
number = "1",
pages = "478-489",
doi = "10.1080/14756366.2016.1261132"
}

Begić, A., Đurić, A., Ninković, M., Stevanović, I., Đurđević, D., Pavlović, M., Jelić, K., Pantelić, A., Zebić, G., Dejanović, B., Stanojević, I., Vojvodić, D., Milosavljević, P., Đukić, M.,& Saso, L.. (2017). Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry
Taylor & Francis Ltd, Abingdon., 32(1), 478-489.
https://doi.org/10.1080/14756366.2016.1261132

Begić A, Đurić A, Ninković M, Stevanović I, Đurđević D, Pavlović M, Jelić K, Pantelić A, Zebić G, Dejanović B, Stanojević I, Vojvodić D, Milosavljević P, Đukić M, Saso L. Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry. 2017;32(1):478-489.
doi:10.1080/14756366.2016.1261132 .

Begić, Aida, Đurić, Ana, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Pavlović, Miloš, Jelić, Katarina, Pantelić, Ana, Zebić, Goran, Dejanović, Bratislav, Stanojević, Ivan, Vojvodić, Danilo, Milosavljević, Petar, Đukić, Mirjana, Saso, Luciano, "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium" in Journal of Enzyme Inhibition and Medicinal Chemistry, 32, no. 1 (2017):478-489,
https://doi.org/10.1080/14756366.2016.1261132 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Stevanović, Ivana
AU  - Lukić, Vera
AU  - Stanojević, Ivan
AU  - Ninković, Milica
AU  - Saso, Luciano
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2952
AB  - The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Chromatographica
T1  - The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue
VL  - 29
IS  - 1
SP  - 67
EP  - 84
DO  - 10.1556/1326.2017.29.1.5
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Gobeljić, Borko and Stevanović, Ivana and Lukić, Vera and Stanojević, Ivan and Ninković, Milica and Saso, Luciano and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Chromatographica",
title = "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue",
volume = "29",
number = "1",
pages = "67-84",
doi = "10.1556/1326.2017.29.1.5"
}

Begić, A., Đurić, A., Gobeljić, B., Stevanović, I., Lukić, V., Stanojević, I., Ninković, M., Saso, L., Vojvodić, D.,& Đukić, M.. (2017). The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica
Akademiai Kiado Rt, Budapest., 29(1), 67-84.
https://doi.org/10.1556/1326.2017.29.1.5

Begić A, Đurić A, Gobeljić B, Stevanović I, Lukić V, Stanojević I, Ninković M, Saso L, Vojvodić D, Đukić M. The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica. 2017;29(1):67-84.
doi:10.1556/1326.2017.29.1.5 .

Begić, Aida, Đurić, Ana, Gobeljić, Borko, Stevanović, Ivana, Lukić, Vera, Stanojević, Ivan, Ninković, Milica, Saso, Luciano, Vojvodić, Danilo, Đukić, Mirjana, "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue" in Acta Chromatographica, 29, no. 1 (2017):67-84,
https://doi.org/10.1556/1326.2017.29.1.5 . .

TY  - JOUR
AU  - Aminjafari, Akram
AU  - Miroliaei, Mehran
AU  - Angelova, Violina T.
AU  - Emamzadeh, Rahman
AU  - Đukić, Mirjana
AU  - Đurić, Ana
AU  - Saso, Luciano
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2679
AB  - Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and beta-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACDmay be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.
PB  - Univ Catolica de Valparaiso, Valparaiso
T2  - Electronic Journal of Biotechnology
T1  - Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
VL  - 24
SP  - 43
EP  - 48
DO  - 10.1016/j.ejbt.2016.08.004
ER  - 

@article{
author = "Aminjafari, Akram and Miroliaei, Mehran and Angelova, Violina T. and Emamzadeh, Rahman and Đukić, Mirjana and Đurić, Ana and Saso, Luciano",
year = "2016",
abstract = "Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and beta-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACDmay be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.",
publisher = "Univ Catolica de Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins",
volume = "24",
pages = "43-48",
doi = "10.1016/j.ejbt.2016.08.004"
}

Aminjafari, A., Miroliaei, M., Angelova, V. T., Emamzadeh, R., Đukić, M., Đurić, A.,& Saso, L.. (2016). Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins. in Electronic Journal of Biotechnology
Univ Catolica de Valparaiso, Valparaiso., 24, 43-48.
https://doi.org/10.1016/j.ejbt.2016.08.004

Aminjafari A, Miroliaei M, Angelova VT, Emamzadeh R, Đukić M, Đurić A, Saso L. Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins. in Electronic Journal of Biotechnology. 2016;24:43-48.
doi:10.1016/j.ejbt.2016.08.004 .

Aminjafari, Akram, Miroliaei, Mehran, Angelova, Violina T., Emamzadeh, Rahman, Đukić, Mirjana, Đurić, Ana, Saso, Luciano, "Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins" in Electronic Journal of Biotechnology, 24 (2016):43-48,
https://doi.org/10.1016/j.ejbt.2016.08.004 . .

TY  - JOUR
AU  - Mancić, Bojana
AU  - Stevanović, Ivana
AU  - Ilić, Tihomir V.
AU  - Đurić, Ana
AU  - Stojanović, Ivana
AU  - Milanović, Slađan
AU  - Ninković, Milica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2639
AB  - Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in activity of excitatory and inhibitory systems as well as redox homeostasis. Our aim was to investigate the effect of single (SS) and repeated session (RS) of intermittentand continuous theta-burst stimulation (iTBS; cTBS) on the expression of vesicular and plasmatic glutamate transporters 1 (vGluT1 and GLT-1), glial fibrillary acidic protein (GFAP) and influence on oxidative status in rats cerebellar tissue and plasma. Redox state parameters in cerebellar tissue and plasma were assessed 24 h after single and 48 h after the last TBS session. Molecular changes were examined by immunofluorescence. Stimulation significantly increased thiol groups (SH) in tissue of SS iTBS group, and decreased in iTBS RS. Activity of glucose-6-phosphate-dehydrogenase (G6PD) was increased markedly in cTBS RS. Immunoreactivity of vGluT1 in cTBS RS decreased, while GLT-1 increased in cTBS SS and cTBS RS, compared to control. Present study gives insight in molecular and biochemical mechanisms by which iTBS and cTBS exerts its effects on rats cerebellar cortex.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neurochemistry International
T1  - Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex
VL  - 100
SP  - 120
EP  - 127
DO  - 10.1016/j.neuint.2016.09.009
ER  - 

@article{
author = "Mancić, Bojana and Stevanović, Ivana and Ilić, Tihomir V. and Đurić, Ana and Stojanović, Ivana and Milanović, Slađan and Ninković, Milica",
year = "2016",
abstract = "Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in activity of excitatory and inhibitory systems as well as redox homeostasis. Our aim was to investigate the effect of single (SS) and repeated session (RS) of intermittentand continuous theta-burst stimulation (iTBS; cTBS) on the expression of vesicular and plasmatic glutamate transporters 1 (vGluT1 and GLT-1), glial fibrillary acidic protein (GFAP) and influence on oxidative status in rats cerebellar tissue and plasma. Redox state parameters in cerebellar tissue and plasma were assessed 24 h after single and 48 h after the last TBS session. Molecular changes were examined by immunofluorescence. Stimulation significantly increased thiol groups (SH) in tissue of SS iTBS group, and decreased in iTBS RS. Activity of glucose-6-phosphate-dehydrogenase (G6PD) was increased markedly in cTBS RS. Immunoreactivity of vGluT1 in cTBS RS decreased, while GLT-1 increased in cTBS SS and cTBS RS, compared to control. Present study gives insight in molecular and biochemical mechanisms by which iTBS and cTBS exerts its effects on rats cerebellar cortex.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neurochemistry International",
title = "Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex",
volume = "100",
pages = "120-127",
doi = "10.1016/j.neuint.2016.09.009"
}

Mancić, B., Stevanović, I., Ilić, T. V., Đurić, A., Stojanović, I., Milanović, S.,& Ninković, M.. (2016). Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex. in Neurochemistry International
Pergamon-Elsevier Science Ltd, Oxford., 100, 120-127.
https://doi.org/10.1016/j.neuint.2016.09.009

Mancić B, Stevanović I, Ilić TV, Đurić A, Stojanović I, Milanović S, Ninković M. Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex. in Neurochemistry International. 2016;100:120-127.
doi:10.1016/j.neuint.2016.09.009 .

Mancić, Bojana, Stevanović, Ivana, Ilić, Tihomir V., Đurić, Ana, Stojanović, Ivana, Milanović, Slađan, Ninković, Milica, "Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex" in Neurochemistry International, 100 (2016):120-127,
https://doi.org/10.1016/j.neuint.2016.09.009 . .

TY  - JOUR
AU  - Đurić, Ana
AU  - Begić, Aida
AU  - Gobeljić, Borko
AU  - Stanojević, Ivan
AU  - Ninković, Milica
AU  - Vojvodić, Danilo
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Prokić, Vera
AU  - Dejanović, Bratislav
AU  - Stojanović, Ivana
AU  - Pavlica, Marina
AU  - Đukić, Dušan
AU  - Saso, Luciano
AU  - Đurđević, Dragan
AU  - Pavlović, Miloš
AU  - Topić, Aleksandra
AU  - Vujanović, Dragana
AU  - Stevnović, Ivana
AU  - Đukić, Mirjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2364
AB  - The objective of our study was to examine testicular toxicity of cadmium (Cd), focusing on oxidative stress (OS), essential metals and androgenic status and morphological changes. Male Wistar rats [controls and four Cd-subgroups (n = 6) organized according to the exposure (1, 3, 10 and 21 days)] were intraperitoneally (i.p.) treated with 1 mg CdCl2/kg/day. Testicular Cd deposition was noticed from the 1st day. After 10 and 21 days, copper (Cu) and iron (Fe) increased by 60-109% and 43-67%, respectively, while zinc (Zn) decreased by 24-33%. During 121 days of the exposure, decrease in testicular total superoxide dismutase (SOD) and total glutathione-s-transferase (GST) activities occurred gradually by 30-78% and 15-84%, respectively, while superoxide anion radical (O-2(center dot-)) increased gradually by 114-271%. After 10-21 days, decrease in testicular catalase (CAT) activity appeared by 13-31%. After 21 days, malondialdehyde (MDA) decreased by 44% and the ratio of oxidized glutathione/reduced glutathione (GSSG/GSH) increased by 130% in testes of the rats exposed to Cd. Additionally, decreased testicular testosterone level and the relative testes mass, along with induced microscopic and macroscopic changes were occured, what can be explained as the consequence of instantly developed OS, impaired essential metals status and Cd testicular deposition.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium
VL  - 86
SP  - 25
EP  - 33
DO  - 10.1016/j.fct.2015.09.004
ER  - 

@article{
author = "Đurić, Ana and Begić, Aida and Gobeljić, Borko and Stanojević, Ivan and Ninković, Milica and Vojvodić, Danilo and Pantelić, Ana and Zebić, Goran and Prokić, Vera and Dejanović, Bratislav and Stojanović, Ivana and Pavlica, Marina and Đukić, Dušan and Saso, Luciano and Đurđević, Dragan and Pavlović, Miloš and Topić, Aleksandra and Vujanović, Dragana and Stevnović, Ivana and Đukić, Mirjana",
year = "2015",
abstract = "The objective of our study was to examine testicular toxicity of cadmium (Cd), focusing on oxidative stress (OS), essential metals and androgenic status and morphological changes. Male Wistar rats [controls and four Cd-subgroups (n = 6) organized according to the exposure (1, 3, 10 and 21 days)] were intraperitoneally (i.p.) treated with 1 mg CdCl2/kg/day. Testicular Cd deposition was noticed from the 1st day. After 10 and 21 days, copper (Cu) and iron (Fe) increased by 60-109% and 43-67%, respectively, while zinc (Zn) decreased by 24-33%. During 121 days of the exposure, decrease in testicular total superoxide dismutase (SOD) and total glutathione-s-transferase (GST) activities occurred gradually by 30-78% and 15-84%, respectively, while superoxide anion radical (O-2(center dot-)) increased gradually by 114-271%. After 10-21 days, decrease in testicular catalase (CAT) activity appeared by 13-31%. After 21 days, malondialdehyde (MDA) decreased by 44% and the ratio of oxidized glutathione/reduced glutathione (GSSG/GSH) increased by 130% in testes of the rats exposed to Cd. Additionally, decreased testicular testosterone level and the relative testes mass, along with induced microscopic and macroscopic changes were occured, what can be explained as the consequence of instantly developed OS, impaired essential metals status and Cd testicular deposition.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium",
volume = "86",
pages = "25-33",
doi = "10.1016/j.fct.2015.09.004"
}

Đurić, A., Begić, A., Gobeljić, B., Stanojević, I., Ninković, M., Vojvodić, D., Pantelić, A., Zebić, G., Prokić, V., Dejanović, B., Stojanović, I., Pavlica, M., Đukić, D., Saso, L., Đurđević, D., Pavlović, M., Topić, A., Vujanović, D., Stevnović, I.,& Đukić, M.. (2015). Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 86, 25-33.
https://doi.org/10.1016/j.fct.2015.09.004

Đurić A, Begić A, Gobeljić B, Stanojević I, Ninković M, Vojvodić D, Pantelić A, Zebić G, Prokić V, Dejanović B, Stojanović I, Pavlica M, Đukić D, Saso L, Đurđević D, Pavlović M, Topić A, Vujanović D, Stevnović I, Đukić M. Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. in Food and Chemical Toxicology. 2015;86:25-33.
doi:10.1016/j.fct.2015.09.004 .

Đurić, Ana, Begić, Aida, Gobeljić, Borko, Stanojević, Ivan, Ninković, Milica, Vojvodić, Danilo, Pantelić, Ana, Zebić, Goran, Prokić, Vera, Dejanović, Bratislav, Stojanović, Ivana, Pavlica, Marina, Đukić, Dušan, Saso, Luciano, Đurđević, Dragan, Pavlović, Miloš, Topić, Aleksandra, Vujanović, Dragana, Stevnović, Ivana, Đukić, Mirjana, "Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium" in Food and Chemical Toxicology, 86 (2015):25-33,
https://doi.org/10.1016/j.fct.2015.09.004 . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Đurić, Ana
AU  - Tasić, Ljiljana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1772
AB  - Abuse of psychoactive substances (PAS) is a major health and social problems, worldwide. The overall population is in direct daily contact with the PAS and thus, at risk of PAS abuse. Pharmacists have a unique and comprehensive knowledge about drugs and PAS, as well as, about the adverse effects of their inappropriate use (abuse). So far, regarding this problematic, the role of pharmacists, as health promoters, is mostly recognized in the field of pharmacotherapy and clinico-toxicological laboratory analysis. However, the role of pharmacists should be recognized more widely in the society, in the sense of prevention, advisory and educational role. According to the American model, in this area of work, pharmacists are responsible for prevention, education and assistance in overall health and social care system of addicts. Also, this paper gives an overview of the importance of institutional harmonization in the PAS domain abuse, at the city, region and state level. Inadequate cooperation at this level is a immense threat to society, and in the same time, an indicator of irresponsibility and neglect of the community regarding addiction related problems.
AB  - Zloupotreba psihoaktivnih supstanci (PAS) je veliki zdravstveno-društveni problem kod nas i u svetu. Sveukupna populacija je u neposrednom svakodnevnom kontaktu sa PAS i izložena je stalnom riziku od PAS. Farmaceuti imaju jedinstveno i sveobuhvatno znanje o bezbednoj i efikasnoj primeni lekova i PAS, kao i o neželjenim efektima do kojih dovodi njihova neadekvatna upotreba (zloupotreba). Uloga farmaceuta, za sada je uglavnom prepoznata u domenu farmakoterapije i kliničko-toksikoloških laboratorijskih analiza. Međutim, uloga farmaceuta kao promotera zdravlja bi trebalo da se prepozna šire u društvu, kada je reč o zloupotrebi i tretmanu zavisnika od PAS, kako u domenu prevencije, tako i tokom lečenja i kasnijoj fazi reintegracije lečenog pacijenta u društvenu zajednicu. Takođe, savetodavna i edukativna uloga farmaceuta u društvu, kod nas je oblast koja je u začetku, kada je reč o zloupotrebi PAS. Prema američkom modelu, odgovornosti farmaceuta u ovom domenu rada podeljene su u tri grupe: prevencija, edukacija i angažman u sveukupnom zdravstveno-socijalnom sistemu zbrinjavanja zavisnika. Takođe, u radu je dat osvrt na značaj institucionalne harmonizacije u domenu zloupotrebe PAS, na nivou grada, regiona i države. Neadekvatna saradnja na ovom nivou je velika pretnja društvu, a i u isto vreme, pokazatelj neodgovornosti i nebrige cele zajednice o problemu zavisnosti kod nas.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Abuse of psychoactive substances: The role of pharmacist
T1  - Zloupotreba psihoaktivnih supstanci - uloga farmaceuta
VL  - 62
IS  - 2
SP  - 179
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1772
ER  - 

@article{
author = "Đukić, Mirjana and Đurić, Ana and Tasić, Ljiljana",
year = "2012",
abstract = "Abuse of psychoactive substances (PAS) is a major health and social problems, worldwide. The overall population is in direct daily contact with the PAS and thus, at risk of PAS abuse. Pharmacists have a unique and comprehensive knowledge about drugs and PAS, as well as, about the adverse effects of their inappropriate use (abuse). So far, regarding this problematic, the role of pharmacists, as health promoters, is mostly recognized in the field of pharmacotherapy and clinico-toxicological laboratory analysis. However, the role of pharmacists should be recognized more widely in the society, in the sense of prevention, advisory and educational role. According to the American model, in this area of work, pharmacists are responsible for prevention, education and assistance in overall health and social care system of addicts. Also, this paper gives an overview of the importance of institutional harmonization in the PAS domain abuse, at the city, region and state level. Inadequate cooperation at this level is a immense threat to society, and in the same time, an indicator of irresponsibility and neglect of the community regarding addiction related problems., Zloupotreba psihoaktivnih supstanci (PAS) je veliki zdravstveno-društveni problem kod nas i u svetu. Sveukupna populacija je u neposrednom svakodnevnom kontaktu sa PAS i izložena je stalnom riziku od PAS. Farmaceuti imaju jedinstveno i sveobuhvatno znanje o bezbednoj i efikasnoj primeni lekova i PAS, kao i o neželjenim efektima do kojih dovodi njihova neadekvatna upotreba (zloupotreba). Uloga farmaceuta, za sada je uglavnom prepoznata u domenu farmakoterapije i kliničko-toksikoloških laboratorijskih analiza. Međutim, uloga farmaceuta kao promotera zdravlja bi trebalo da se prepozna šire u društvu, kada je reč o zloupotrebi i tretmanu zavisnika od PAS, kako u domenu prevencije, tako i tokom lečenja i kasnijoj fazi reintegracije lečenog pacijenta u društvenu zajednicu. Takođe, savetodavna i edukativna uloga farmaceuta u društvu, kod nas je oblast koja je u začetku, kada je reč o zloupotrebi PAS. Prema američkom modelu, odgovornosti farmaceuta u ovom domenu rada podeljene su u tri grupe: prevencija, edukacija i angažman u sveukupnom zdravstveno-socijalnom sistemu zbrinjavanja zavisnika. Takođe, u radu je dat osvrt na značaj institucionalne harmonizacije u domenu zloupotrebe PAS, na nivou grada, regiona i države. Neadekvatna saradnja na ovom nivou je velika pretnja društvu, a i u isto vreme, pokazatelj neodgovornosti i nebrige cele zajednice o problemu zavisnosti kod nas.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Abuse of psychoactive substances: The role of pharmacist, Zloupotreba psihoaktivnih supstanci - uloga farmaceuta",
volume = "62",
number = "2",
pages = "179-189",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1772"
}

Đukić, M., Đurić, A.,& Tasić, L.. (2012). Abuse of psychoactive substances: The role of pharmacist. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(2), 179-189.
https://hdl.handle.net/21.15107/rcub_farfar_1772

Đukić M, Đurić A, Tasić L. Abuse of psychoactive substances: The role of pharmacist. in Arhiv za farmaciju. 2012;62(2):179-189.
https://hdl.handle.net/21.15107/rcub_farfar_1772 .

Đukić, Mirjana, Đurić, Ana, Tasić, Ljiljana, "Abuse of psychoactive substances: The role of pharmacist" in Arhiv za farmaciju, 62, no. 2 (2012):179-189,
https://hdl.handle.net/21.15107/rcub_farfar_1772 .