TY  - JOUR
AU  - Mijatović, Stefan
AU  - Antić-Stanković, Jelena
AU  - Čolović Čalovski, Ivana, Ivana
AU  - Dubljanin, Eleonora
AU  - Pljevljakušić, Dejan
AU  - Bigović, Dubravka
AU  - Džamić, Aleksandar
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4292
AB  - The aim of our study was to determine the susceptibility of 15 Candida albicans sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of Lavandula angustifolia essential oil (LAEO). The commercial LAEO was analyzed using gas chromatography-mass spectrometry. The antifungal activity was evaluated using EUCAST protocol. A killing assay was performed to evaluate kinetics of 2% LAEO within 30 min treatment. LAEO with major constituents’ linalool (33.4%) and linalyl acetate (30.5%) effective inhibited grows of C. albicans in concentration range 0.5–2%. Fluconazole activity was noted in 67% of the isolates with MICs in range 0.06–1 µg/mL. Surprisingly, 40% of isolates were non-wild-type (non-WT), while MICs for WT ranged between 0.125–0.25 µg/mL. There were no significant differences in the LAEO MICs among fluconazole-resistant and fluconazole-susceptible sputum strains (p = 0.31) and neither among caspofungin non-WT and WT isolates (p = 0.79). The 2% LAEO rapidly achieved 50% growth reduction in all tested strains between 0.2 and 3.5 min. Within 30 min, the same LAEO concentration exhibited a 99.9% reduction in 27% isolates. This study demonstrated that 2% solution of LAEO showed a significant antifungal activity which is equally effective against fluconazole and caspofungin susceptible and less-susceptible strains.
PB  - MDPI
T2  - Molecules
T1  - Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates
VL  - 27
IS  - 19
DO  - 10.3390/molecules27196300
ER  - 

@article{
author = "Mijatović, Stefan and Antić-Stanković, Jelena and Čolović Čalovski, Ivana, Ivana and Dubljanin, Eleonora and Pljevljakušić, Dejan and Bigović, Dubravka and Džamić, Aleksandar",
year = "2022",
abstract = "The aim of our study was to determine the susceptibility of 15 Candida albicans sputum isolates on fluconazole and caspofungin, as well as the antifungal potential of Lavandula angustifolia essential oil (LAEO). The commercial LAEO was analyzed using gas chromatography-mass spectrometry. The antifungal activity was evaluated using EUCAST protocol. A killing assay was performed to evaluate kinetics of 2% LAEO within 30 min treatment. LAEO with major constituents’ linalool (33.4%) and linalyl acetate (30.5%) effective inhibited grows of C. albicans in concentration range 0.5–2%. Fluconazole activity was noted in 67% of the isolates with MICs in range 0.06–1 µg/mL. Surprisingly, 40% of isolates were non-wild-type (non-WT), while MICs for WT ranged between 0.125–0.25 µg/mL. There were no significant differences in the LAEO MICs among fluconazole-resistant and fluconazole-susceptible sputum strains (p = 0.31) and neither among caspofungin non-WT and WT isolates (p = 0.79). The 2% LAEO rapidly achieved 50% growth reduction in all tested strains between 0.2 and 3.5 min. Within 30 min, the same LAEO concentration exhibited a 99.9% reduction in 27% isolates. This study demonstrated that 2% solution of LAEO showed a significant antifungal activity which is equally effective against fluconazole and caspofungin susceptible and less-susceptible strains.",
publisher = "MDPI",
journal = "Molecules",
title = "Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates",
volume = "27",
number = "19",
doi = "10.3390/molecules27196300"
}

Mijatović, S., Antić-Stanković, J., Čolović Čalovski, I. I., Dubljanin, E., Pljevljakušić, D., Bigović, D.,& Džamić, A.. (2022). Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates. in Molecules
MDPI., 27(19).
https://doi.org/10.3390/molecules27196300

Mijatović S, Antić-Stanković J, Čolović Čalovski II, Dubljanin E, Pljevljakušić D, Bigović D, Džamić A. Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates. in Molecules. 2022;27(19).
doi:10.3390/molecules27196300 .

Mijatović, Stefan, Antić-Stanković, Jelena, Čolović Čalovski, Ivana, Ivana, Dubljanin, Eleonora, Pljevljakušić, Dejan, Bigović, Dubravka, Džamić, Aleksandar, "Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196300 . .

TY  - CONF
AU  - Kuzmanović Nedeljković, Snežana
AU  - Ćujić Nikolić, Nada
AU  - Bigović, Dubravka
AU  - Petrović, Predrag
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Filipić, Brankica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5120
AB  - Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
T1  - Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5120
ER  - 

@conference{
author = "Kuzmanović Nedeljković, Snežana and Ćujić Nikolić, Nada and Bigović, Dubravka and Petrović, Predrag and Pljevljakušić, Dejan and Šavikin, Katarina and Filipić, Brankica",
year = "2022",
abstract = "Among plant natural products, Oregano and Thyme essential oils (EOs) are well-known for 
their antimicrobial and antioxidant activity. The biological activities of EOs may be reduced 
due to oxidation and volatilization. Stability and preservation of EOs can be assured with the 
microencapsulation method based on complex coacervation technology. Cyclodextrins are 
suitable as carriers for volatile substances insoluble in water, and hydroxypropyl-β cyclodextrin (HPCD) leads the group considering the aqueous solubility and safe toxicity 
profile. The aim of this study was to find conditions for preparing Oregano and Thyme EOs 
microencapsulates with the best technological properties.Oregano and Thyme EOs were 
encapsulated by the freeze drying (lyophilization) method. Nine different combinations were 
prepared, where HPCD content (10, 15 and 20%) and EO:HPCD mass ratio (1:1, 1:5 and 
1:10) were varied. After stirring (200 rpm) for 24 h at a room temperature, suspensions were 
filtered through 0.45 mm PTFE filters. Samples were evaporated under vacuum and frozen (-
80 °C for 1 h), then main drying was carried out (-60 °C, pressure of 0.011 mbar for 40 h), 
and final drying (-65 °C, pressure of 0.054 mbar for 1 h). To ensure the particle purity, 
lyophilizates were washed with acetonitrile and dried at 25 °C. The content of each EO was 
determined spectrophotometrically, encapsulation efficiency (EE%) and yield were 
calculated. Duncan’s post hoc test was used to evaluate the differences between samples. 
The best conditions for both EOs were obtained with 15% HPCD and EO:HPCD mass ratio 
of 1:10. Yields of EOs for chosen Oregano and Thyme EOs complexes were 86.81 ± 2.20% 
and 89.83 ± 2.80%, respectively, with the following EE% of 49.08 ± 1.80% and 49.29 ± 
0.18%. Microencapsulation is a promising method for improving the EOs stability profile, 
and these results could be very useful in the pharmaceutical and food industry for the 
implementation of new products.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts",
title = "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5120"
}

Kuzmanović Nedeljković, S., Ćujić Nikolić, N., Bigović, D., Petrović, P., Pljevljakušić, D., Šavikin, K.,& Filipić, B.. (2022). Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts
Belgrade : Institute of Technical Sciences of SASA., 14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120

Kuzmanović Nedeljković S, Ćujić Nikolić N, Bigović D, Petrović P, Pljevljakušić D, Šavikin K, Filipić B. Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin. in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts. 2022;:14-14.
https://hdl.handle.net/21.15107/rcub_farfar_5120 .

Kuzmanović Nedeljković, Snežana, Ćujić Nikolić, Nada, Bigović, Dubravka, Petrović, Predrag, Pljevljakušić, Dejan, Šavikin, Katarina, Filipić, Brankica, "Microencapsulation of Oregano and Thyme essential oils with hydroxypropyl-β cyclodextrin" in Twentieth Young Researchers Conference – Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia - Programme and the Book of Abstracts (2022):14-14,
https://hdl.handle.net/21.15107/rcub_farfar_5120 .