@article{
author = "Jeremić, Aleksandra and Milosavljević, Filip and Vladimirov, Sandra and Batinić, Bojan and Marković, Bojan and Jukić, Marin",
year = "2021",
abstract = "Simultaneous quantification of multiple psychiatric drugs is important for the therapeutic
drug monitoring of psychiatric patients. In addition, it would be highly advantageous if the
method could be simple, straightforward, and not time-consuming. A 200 µl plasma sample was
deproteinized, drugs were separated by a ZORBAX Eclipse XDB-Phenyl column with the
mobile phase composed of acetonitrile and 0.1 % formic acid in water (60:40, v/v), and recorded
in the MRM mode by using a positive electrospray source with tandem mass spectrometry
detection. The dynamic range was 2–256 ng/ml for all the analyzed drugs, except escitalopram
(8-256 ng/ml). Quality control samples were prepared in quintuplicates in three relevant
concentrations for each drug. Coefficients of determination (R2
) were higher than 0.99, while the
relative difference between nominal and measured concentrations (RE) and CV were lower than
15% for all targets. High performance liquid chromatography coupled with the mass detector
(HPLC-MS/MS) method for simultaneous determination of sertraline, escitalopram, risperidone
366
and paliperidone in human plasma was validated with respect to selectivity, linearity, accuracy,
precision, matrix effect and stability. This method has significant advantages in terms of low
sample volume (200 µl), short preparation time (3 hours) and short runtime per sample (4
minutes)., Simultana kvantifikacija većeg broja psihijatrijskih lekova je
važna za terapijsko praćenje
psihijatrijskih pacijenata. Takođe
, od značaja iz praktičnih ra
zloga bi bilo da metoda bude
jednostavna, laka za izvođenje i
da ne zahteva puno vremena. Uz
orak plazme (200 μl) je
deproteinizovan i izvršeno je h
romatografsko razdvajanje lekova
na
ZORBAX Eclipse XDB-
Phenyl koloni sa mobilnom fazom sačinjenom od acetonitrila i vo
denog rastvora
0,1%
mravlje kiseline
(
60:40, v/v). Maseni spektri snimani su u MRM modu korišćenjem i
zvora sa
elektrosprej pozitivnom jonizacijom i tandemske masene detekcij
e. Ispitivani opseg koncentracija
bio je 2–256 ng/ml za sve analizirane lekove, osim za escitalop
ram, gde je bio 8-256 ng/ml.
Kontrolni uzorci su pripremani u
kvintuplikatu u tri relevantne
koncentracije za svaki lek.
Determinacioni koeficijenti (R
2
) bili su veći od 0,99, dok su relativna razlika između nominal
nih
i merenih koncentracija (RV) i koeficijent varijacije (CV) bili
manji od 15% za sve analite.
Metoda tečne hromatografije visoki
h performansi uparena sa tand
em masenim detektorom
(HPLC-MS/MS) je validirana u pogledu selektivnosti, linearnosti
, tačnosti, preciznosti, efekata
matriksa i stabilnosti za simultano određivanje
sertralina, escitaloprama, risperidona i
paliperidona u humanoj plazmi. Metoda zahteva malu zapreminu uz
orka (200 μl), kratke pripreme
uzorka (3 sata) i kratko vreme tr
ajanja pojedinačnog merenja (4
minuta).",
journal = "Arhiv za farmaciju",
title = "Validation of a quick and simple chromatographic method for simultaneous quantification of sertraline, escitalopram, risperidone and paliperidone levels in the human plasma, Validacija brze i jednostavne hromatografske
metode za simultanu kvantifikaciju nivoa
sertralina, escitaloprama, risperidona i
paliperidona u humanoj plazmi",
volume = "71",
number = "5",
pages = "365-377",
doi = "10.5937/arhfarm71-31163"
}