Potparević, Biljana

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Authority KeyName Variants
orcid::0000-0001-5570-883X
  • Potparević, Biljana (62)
  • Spremo-Potparević, Biljana (49)
  • Spremo-Potparević, Biljana (1)
Projects
Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200161 (University of Belgrade, Faculty of Pharmacy)
Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa Hormonal regulation of expression and activity of the nitric oxide synthase and sodium-potassium pump in experimental models of insulin resistance, diabetes and cardiovascular disorders
Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production Characterization and application of fungal metabolites and assessment of new biofungicides potential
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion
COST Action CA15132 (hCOMET), supported by COST (European Cooperation in Science and Technology). EU COST Action CA 15132
hCOMET COST action (No 15132) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200043 (Institute of Oncology and Radiology of Serbia, Belgrade)
BU-RS/16-17-019 COMET COST action (15132)
Computational Bioscience Research Center (CBRC) Federal Republic of Germany
hCOMET COST action (No. 15132). hCOMET COST (Action No. CA 15132)
hCOMET COST (Action No CA 15132) Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan
Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200015 (University of Belgrade, Institute for Medical Research)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200125 (University of Novi Sad, Faculty of Science) "info:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Carotid disease in Serbia - pathologic dynamics, prevention, diagnostics and inovative therapeutic methods
Ecophysiological and genetic investigations of domestic animals and bees for the purpose of increasing reproductive traits and disease resistance Development of micro- and nanosystems as carriers for drugs with anti-inflammatory effect and methods for their characterization

Author's Bibliography

Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach

Selaković, Snežana; Rodić, Marko; Novaković, Irena; Matić, Ivana; Stanojković, Tatjana; Pirković, Andrea; Živković, Lada; Spremo-Potparević, Biljana; Milčić, Vesna; Dimiza, Filitsa; Psomas, George; Anđelković, Katarina; Šumar-Ristović, Maja

(Royal Society of Chemistry, 2024)

TY  - JOUR
AU  - Selaković, Snežana
AU  - Rodić, Marko
AU  - Novaković, Irena
AU  - Matić, Ivana
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Milčić, Vesna
AU  - Dimiza, Filitsa
AU  - Psomas, George
AU  - Anđelković, Katarina
AU  - Šumar-Ristović, Maja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5522
AB  - Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.
PB  - Royal Society of Chemistry
T2  - Dalton Transactions
T1  - Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach
VL  - 53
IS  - 6
SP  - 2770
EP  - 2788
DO  - 10.1039/d3dt03862a
ER  - 
@article{
author = "Selaković, Snežana and Rodić, Marko and Novaković, Irena and Matić, Ivana and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Milčić, Vesna and Dimiza, Filitsa and Psomas, George and Anđelković, Katarina and Šumar-Ristović, Maja",
year = "2024",
abstract = "Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.",
publisher = "Royal Society of Chemistry",
journal = "Dalton Transactions",
title = "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach",
volume = "53",
number = "6",
pages = "2770-2788",
doi = "10.1039/d3dt03862a"
}
Selaković, S., Rodić, M., Novaković, I., Matić, I., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Milčić, V., Dimiza, F., Psomas, G., Anđelković, K.,& Šumar-Ristović, M.. (2024). Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions
Royal Society of Chemistry., 53(6), 2770-2788.
https://doi.org/10.1039/d3dt03862a
Selaković S, Rodić M, Novaković I, Matić I, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Milčić V, Dimiza F, Psomas G, Anđelković K, Šumar-Ristović M. Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions. 2024;53(6):2770-2788.
doi:10.1039/d3dt03862a .
Selaković, Snežana, Rodić, Marko, Novaković, Irena, Matić, Ivana, Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Milčić, Vesna, Dimiza, Filitsa, Psomas, George, Anđelković, Katarina, Šumar-Ristović, Maja, "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach" in Dalton Transactions, 53, no. 6 (2024):2770-2788,
https://doi.org/10.1039/d3dt03862a . .
3

New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease

Spremo-Potparević, Biljana; Želježić, Davor; Bajić, Vladan; Popović, Petar; Živković, Lada

(Macedonian Academy of Sciences and Arts, 2023)

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Želježić, Davor
AU  - Bajić, Vladan
AU  - Popović, Petar
AU  - Živković, Lada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5471
AB  - Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.
PB  - Macedonian Academy of Sciences and Arts
C3  - 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
T1  - New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease
VL  - 26
IS  - Supplement
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5471
ER  - 
@conference{
author = "Spremo-Potparević, Biljana and Želježić, Davor and Bajić, Vladan and Popović, Petar and Živković, Lada",
year = "2023",
abstract = "Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK",
title = "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease",
volume = "26",
number = "Supplement",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5471"
}
Spremo-Potparević, B., Želježić, D., Bajić, V., Popović, P.,& Živković, L.. (2023). New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
Macedonian Academy of Sciences and Arts., 26(Supplement), 88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471
Spremo-Potparević B, Želježić D, Bajić V, Popović P, Živković L. New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK. 2023;26(Supplement):88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471 .
Spremo-Potparević, Biljana, Želježić, Davor, Bajić, Vladan, Popović, Petar, Živković, Lada, "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease" in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK, 26, no. Supplement (2023):88-88,
https://hdl.handle.net/21.15107/rcub_farfar_5471 .

Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes

Pirković, Andrea; Lazić, Vesna; Spremo-Potparević, Biljana; Živković, Lada; Topalović, Dijana; Kuzman, Sanja; Antić-Stanković, Jelena; Božić, Dragana; Jovanović Krivokuća, Milica; Nedeljković, Jovan M.

(Oxford University Press, 2023)

TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4919
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 
@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}
Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J. M.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis
Oxford University Press., 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013
Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković JM. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .
Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan M., "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .
1

Evaluation of the antioxidant potential of Biochaga in vitro

Živković, Lada; Topalović, Dijana; Bajić, Vladan; Spremo-Potparević, Biljana

(Macedonian Academy of Sciences and Arts, 2023)

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5185
AB  - Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.
PB  - Macedonian Academy of Sciences and Arts
C3  - Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
T1  - Evaluation of the antioxidant potential of Biochaga in vitro
VL  - 26
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5185
ER  - 
@conference{
author = "Živković, Lada and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Background: Antioxidants and prooxidants have an impact on the intracellular oxidative equilibrium. Overproduction of prooxidants leads to oxidative stress caused by imbalances in oxidative reduction pathways. The body can be supplied with non-enzymatic, low molecular weight antioxidants through diet.The edible medicinal mushroom Chaga, Inonotus obliquus (Ach. ex Pers.) Pilat, has long been long used to treat or prevent various health conditions and disorders. The bioactive compounds of Chaga exhibit antitumor, anti-inflammatory, hypoglycemic, immunomodulatory, antioxidant, and antigenotoxic effects. Material and Methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity, FRAP (ferric reducing antioxidant power) total antioxidant activity, and hydroxyl radical scavenging capacity were measured.
Results: Commercial Biochaga (B), a water extract of Biochaga mushroom, was obtained from Sibpribor Ooo, Irkutsk, Russia. B (IC =5.9 mg/ mL) showed moderate reducing power compared in comparison to vitamin C and strong compared to BTH. B (IC =1.78 mg/mL) showed remarkable free radical scavenging and moderate hydroxyl scavenging activity (IC =8.473 mg/mL). Conclusion: We can place Biochaga in the radical scavenging category because it efficiently eliminates hydroxyl radicals against which the body has insufficient antioxidant defenses.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)",
title = "Evaluation of the antioxidant potential of Biochaga in vitro",
volume = "26",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5185"
}
Živković, L., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book)
Macedonian Academy of Sciences and Arts., 26, 146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185
Živković L, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of the antioxidant potential of Biochaga in vitro. in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book). 2023;26:146-146.
https://hdl.handle.net/21.15107/rcub_farfar_5185 .
Živković, Lada, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of the antioxidant potential of Biochaga in vitro" in Balkan Journal of Medical Genetics, Supplement 1 (Abstract book), 26 (2023):146-146,
https://hdl.handle.net/21.15107/rcub_farfar_5185 .

Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist

Živković, Lada; Borozan, Sunčica; Topalović, Dijana; Bajić, Vladan; Spremo-Potparević, Biljana

(European Environmental Mutagenesis and Genomics Society (EEMGS), 2023)

TY  - CONF
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4748
AB  - Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist
SP  - 46
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4748
ER  - 
@conference{
author = "Živković, Lada and Borozan, Sunčica and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2023",
abstract = "Mushrooms have been evaluated for their nutritional and medicinal properties for
centuries. We evaluated the biological activities of commercial products of Cordyceps
Sinensis (CS), Agaricus blazei (AB), and Immune Assist (IA) (all produced by Aloha
Medicinals). Immune Assist (IA) is made from extract of six species of medical
mushrooms: Agaricus blazei - Cordycepssinensis - Grifola frondosa - Ganoderma
lucidum - Coriolus versicolor - Lentinula edodes.
The antioxidant evaluation showed that CS had strong OH scavenging properties and
moderate reducing power, while its DPPH scavenging ability was weak. AB displayed
remarkable ·OH scavenging properties, moderate reducing power, and modest DPPH
scavenging activity, similar to IA. Also, CS, AB and IA displayed significant antigenotoxic
effects in human peripheral blood cells against H2O2-induced DNA damage.
Further, the cytotoxicity of CS, AB, and IA to HS-5 (bone marrow stroma cells), MCF-
7 (human breast epithelial cell line), and MDA-MB-231 (human breast carcinoma cell
line) were assessed using MTT assay. AB and IA could inhibit the proliferative action
of mentioned cancer cell lines after 72h in a dose-dependent manner, while there were
no effects on HS-5 cell lines proliferation, while CS did not show anti-proliferative/
cytotoxic activity on cancer cell lines.
Current findings remain a significant challenge for the usage of medicinal mushrooms
in the field of cancer prevention/treatment, and thus an active area of future research.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4748"
}
Živković, L., Borozan, S., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2023). Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748
Živković L, Borozan S, Topalović D, Bajić V, Spremo-Potparević B. Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:46-46.
https://hdl.handle.net/21.15107/rcub_farfar_4748 .
Živković, Lada, Borozan, Sunčica, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Evaluation of antioxidative, antigenotoxic, and anticancer activities of commercial medical mushrooms products: Agaricus blazei, Cordyceps Sinensis and Immune Assist" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):46-46,
https://hdl.handle.net/21.15107/rcub_farfar_4748 .

Prospective study of cytotoxic and genotoxic effects of Combretastatin

Spremo-Potparević, Biljana; Topalović, Dijana; Živković, Lada; Marković, Milica; Pirković, Andrea

(European Environmental Mutagenesis and Genomics Society (EEMGS), 2023)

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Marković, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4747
AB  - Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.
PB  - European Environmental Mutagenesis and Genomics Society (EEMGS)
C3  - 51st EEMGS and 27th SEMA meeting: Abstract book
T1  - Prospective study of cytotoxic and genotoxic effects of Combretastatin
SP  - 133
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4747
ER  - 
@conference{
author = "Spremo-Potparević, Biljana and Topalović, Dijana and Živković, Lada and Marković, Milica and Pirković, Andrea",
year = "2023",
abstract = "Combretastatins are a class of natural phenols found in the bark of Combretum
caffrum, commonly known as South African Bush Willow. Despite having a similar
name, combretastatins are unrelated to statins, a family of cholesterol-lowering drugs.
Combretastatin A4 have been shown to be one of the most potent tubulin-depolymerizing
agent. Microtubules control chromosomal segregation and cytokinesis during mitosis
in both cancer and stromal cells and contribute to overall tumor growth. Consequently,
microtubule inhibitors interfere with cell cycle progression and induce apoptosis in
cancer cells in vitro.
The aim of this study was to investigate the potential gentoxic effect of Comretastatin
A4 (CA4) in isolated peripheral blood mononuclear cells (PBMC) in Comet assay in
order to establish is there any DNA damage in healty non-dividing cells. The aim also
was to explore potential cytotoxic activity of CA4 against human cervical carcinoma
(HeLa) cell line.
Genotoxicity of CA4 was evaluated on PBMC in a range of 9 concentrations
(from 1 nM to 200μM). Non of the tested concentrations showed genotoxiceffect.
The same range of different concentrations of CA4 (from 1 nM to 200μM) were
applied to evaluate potential cytotoxicity in a monolayer culture of HeLa cells using
the 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
After 24h incubation with CA4, there was a significant reduction in cell viability in all
concentrations above 250 nm, while IC50 (half maximal inhibitory concentration) was
123 ± 0.06396 μM.
We concluded that CA4 does not have gentoxic effect on PBMC, and that it reduce
cell viability of cancer HeLa cell lines. These results are especialy importanat because
they showed that CA4 does not damage the DNA molecule in healthy human cells, but
achieves its cytotoxic effect on malignant cells in the same range of concentrations.",
publisher = "European Environmental Mutagenesis and Genomics Society (EEMGS)",
journal = "51st EEMGS and 27th SEMA meeting: Abstract book",
title = "Prospective study of cytotoxic and genotoxic effects of Combretastatin",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4747"
}
Spremo-Potparević, B., Topalović, D., Živković, L., Marković, M.,& Pirković, A.. (2023). Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book
European Environmental Mutagenesis and Genomics Society (EEMGS)., 133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747
Spremo-Potparević B, Topalović D, Živković L, Marković M, Pirković A. Prospective study of cytotoxic and genotoxic effects of Combretastatin. in 51st EEMGS and 27th SEMA meeting: Abstract book. 2023;:133-133.
https://hdl.handle.net/21.15107/rcub_farfar_4747 .
Spremo-Potparević, Biljana, Topalović, Dijana, Živković, Lada, Marković, Milica, Pirković, Andrea, "Prospective study of cytotoxic and genotoxic effects of Combretastatin" in 51st EEMGS and 27th SEMA meeting: Abstract book (2023):133-133,
https://hdl.handle.net/21.15107/rcub_farfar_4747 .

Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract

Spremo-Potparević, Biljana; Živković, Lada; Topalović, Dijana; Kotur-Stevuljević, Jelena; Samardžić, Stevan; Maksimović, Zoran

(Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands, 2022)

TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kotur-Stevuljević, Jelena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4618
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4618
ER  - 
@conference{
author = "Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kotur-Stevuljević, Jelena and Samardžić, Stevan and Maksimović, Zoran",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4618"
}
Spremo-Potparević, B., Živković, L., Topalović, D., Kotur-Stevuljević, J., Samardžić, S.,& Maksimović, Z.. (2022). Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618
Spremo-Potparević B, Živković L, Topalović D, Kotur-Stevuljević J, Samardžić S, Maksimović Z. Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:90-90.
https://hdl.handle.net/21.15107/rcub_farfar_4618 .
Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kotur-Stevuljević, Jelena, Samardžić, Stevan, Maksimović, Zoran, "Antioxidant, genotoxic and antigenotoxic properties of Ambrosia artemisiifolia L. (Asteraceae) deodorized water extract" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):90-90,
https://hdl.handle.net/21.15107/rcub_farfar_4618 .

Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin

Sredojević, Dušan; Lazić, Vesna; Pirković, Andrea; Periša, Jovana; Murafa, Natalija; Spremo-Potparević, Biljana; Živković, Lada; Topalović, Dijana; Zarubica, Aleksandra; Jovanović Krivokuća, Milica; Nedeljković, Jovan

(MDPI, 2022)

TY  - JOUR
AU  - Sredojević, Dušan
AU  - Lazić, Vesna
AU  - Pirković, Andrea
AU  - Periša, Jovana
AU  - Murafa, Natalija
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Zarubica, Aleksandra
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4281
AB  - The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control
PB  - MDPI
T2  - Nanomaterials
T1  - Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin
VL  - 12
IS  - 18
DO  - 10.3390/nano12183195
ER  - 
@article{
author = "Sredojević, Dušan and Lazić, Vesna and Pirković, Andrea and Periša, Jovana and Murafa, Natalija and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Zarubica, Aleksandra and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2022",
abstract = "The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control",
publisher = "MDPI",
journal = "Nanomaterials",
title = "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin",
volume = "12",
number = "18",
doi = "10.3390/nano12183195"
}
Sredojević, D., Lazić, V., Pirković, A., Periša, J., Murafa, N., Spremo-Potparević, B., Živković, L., Topalović, D., Zarubica, A., Jovanović Krivokuća, M.,& Nedeljković, J.. (2022). Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials
MDPI., 12(18).
https://doi.org/10.3390/nano12183195
Sredojević D, Lazić V, Pirković A, Periša J, Murafa N, Spremo-Potparević B, Živković L, Topalović D, Zarubica A, Jovanović Krivokuća M, Nedeljković J. Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials. 2022;12(18).
doi:10.3390/nano12183195 .
Sredojević, Dušan, Lazić, Vesna, Pirković, Andrea, Periša, Jovana, Murafa, Natalija, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Zarubica, Aleksandra, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin" in Nanomaterials, 12, no. 18 (2022),
https://doi.org/10.3390/nano12183195 . .
2
2

DES-Amyloidoses “Amyloidoses through the looking-glass”: A knowledgebase developed for exploring and linking information related to human amyloid-related diseases

Bajić, Vladan; Salhi, Adil; Lakota, Katja; Radovanović, Aleksandar; Razali, Rozaimi; Živković, Lada; Spremo-Potparević, Biljana; Uludag, Mahmut; Tifratene, Faroug; Motwalli, Olaa; Marchand, Benoit; Bajić, Vladimir; Gojobori, Takashi; Isenović, Esma; Essack, Magbubah

(Public Library of Science, 2022)

TY  - JOUR
AU  - Bajić, Vladan
AU  - Salhi, Adil
AU  - Lakota, Katja
AU  - Radovanović, Aleksandar
AU  - Razali, Rozaimi
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Uludag, Mahmut
AU  - Tifratene, Faroug
AU  - Motwalli, Olaa
AU  - Marchand, Benoit
AU  - Bajić, Vladimir
AU  - Gojobori, Takashi
AU  - Isenović, Esma
AU  - Essack, Magbubah
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4249
AB  - More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases
VL  - 17
IS  - 7
DO  - 10.1371/journal.pone.0271737
ER  - 
@article{
author = "Bajić, Vladan and Salhi, Adil and Lakota, Katja and Radovanović, Aleksandar and Razali, Rozaimi and Živković, Lada and Spremo-Potparević, Biljana and Uludag, Mahmut and Tifratene, Faroug and Motwalli, Olaa and Marchand, Benoit and Bajić, Vladimir and Gojobori, Takashi and Isenović, Esma and Essack, Magbubah",
year = "2022",
abstract = "More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases",
volume = "17",
number = "7",
doi = "10.1371/journal.pone.0271737"
}
Bajić, V., Salhi, A., Lakota, K., Radovanović, A., Razali, R., Živković, L., Spremo-Potparević, B., Uludag, M., Tifratene, F., Motwalli, O., Marchand, B., Bajić, V., Gojobori, T., Isenović, E.,& Essack, M.. (2022). DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases. in PLoS ONE
Public Library of Science., 17(7).
https://doi.org/10.1371/journal.pone.0271737
Bajić V, Salhi A, Lakota K, Radovanović A, Razali R, Živković L, Spremo-Potparević B, Uludag M, Tifratene F, Motwalli O, Marchand B, Bajić V, Gojobori T, Isenović E, Essack M. DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases. in PLoS ONE. 2022;17(7).
doi:10.1371/journal.pone.0271737 .
Bajić, Vladan, Salhi, Adil, Lakota, Katja, Radovanović, Aleksandar, Razali, Rozaimi, Živković, Lada, Spremo-Potparević, Biljana, Uludag, Mahmut, Tifratene, Faroug, Motwalli, Olaa, Marchand, Benoit, Bajić, Vladimir, Gojobori, Takashi, Isenović, Esma, Essack, Magbubah, "DES-Amyloidoses “Amyloidoses through the
looking-glass”: A knowledgebase developed
for exploring and linking information related
to human amyloid-related diseases" in PLoS ONE, 17, no. 7 (2022),
https://doi.org/10.1371/journal.pone.0271737 . .
18

Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins

Vitomirov, Teodora; Dimiza, Filitsa; Matić, Ivana Z.; Stanojković, Tatjana; Pirković, Andrea; Živković, Lada; Spremo-Potparević, Biljana; Novaković, Irena; Anđelković, Katarina; Milčić, Miloš; Psomas, George; Šumar Ristović, Maja

(Elsevier Inc., 2022)

TY  - JOUR
AU  - Vitomirov, Teodora
AU  - Dimiza, Filitsa
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Novaković, Irena
AU  - Anđelković, Katarina
AU  - Milčić, Miloš
AU  - Psomas, George
AU  - Šumar Ristović, Maja
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4248
AB  - In this article, cytotoxicity, the mechanisms of cytotoxic activity, genotoxicity, and interaction with DNA and proteins, of two Cu(II) complexes with a salicylaldehyde derivative (4-(diethylamino)salicylaldehyde) and α-diimine (2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen)) are reported. Both Cu(II) complexes performed cytotoxic effects against all tested malignant cell lines. Complexes exerted highest cytotoxicity against HeLa and A375 malignant cell lines. The cytotoxic activity of Cu(II) complex with phen as a α-diimine co-ligand was significantly higher in comparison with cytotoxic activity of Cu(II) complex with bipy. Pretreatment with specific inhibitors of caspase-3, caspase-8 or caspase-9, in order to clear up the mode of cell death triggered by two Cu(II) complexes in HeLa cells, indicated the ability of these complexes to induce apoptosis through activation of target caspases. Cu(II)-phen complex exhibited significant antioxidant activity compared with Cu(II)-bipy complex, and showed a better effect on reducing intracellular ROS levels in HeLa cells. Tested complexes did not display genotoxic potential in human peripheral blood leucocytes, but exhibited an antigenotoxic effect in post-treatment, after H2O2 exposure. The study of the in vitro biological properties regarding their affinity towards CT (calf-thymus) DNA and serum albumins showed that the compounds can intercalate to CT DNA, and bind reversibly and tightly to the albumins. Molecular docking studies of the ability of compounds to bind to biomacromolecules are consistent with in vitro studies.
PB  - Elsevier Inc.
T2  - Journal of Inorganic Biochemistry
T1  - Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins
VL  - 235
DO  - 10.1016/j.jinorgbio.2022.111942
ER  - 
@article{
author = "Vitomirov, Teodora and Dimiza, Filitsa and Matić, Ivana Z. and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Novaković, Irena and Anđelković, Katarina and Milčić, Miloš and Psomas, George and Šumar Ristović, Maja",
year = "2022",
abstract = "In this article, cytotoxicity, the mechanisms of cytotoxic activity, genotoxicity, and interaction with DNA and proteins, of two Cu(II) complexes with a salicylaldehyde derivative (4-(diethylamino)salicylaldehyde) and α-diimine (2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen)) are reported. Both Cu(II) complexes performed cytotoxic effects against all tested malignant cell lines. Complexes exerted highest cytotoxicity against HeLa and A375 malignant cell lines. The cytotoxic activity of Cu(II) complex with phen as a α-diimine co-ligand was significantly higher in comparison with cytotoxic activity of Cu(II) complex with bipy. Pretreatment with specific inhibitors of caspase-3, caspase-8 or caspase-9, in order to clear up the mode of cell death triggered by two Cu(II) complexes in HeLa cells, indicated the ability of these complexes to induce apoptosis through activation of target caspases. Cu(II)-phen complex exhibited significant antioxidant activity compared with Cu(II)-bipy complex, and showed a better effect on reducing intracellular ROS levels in HeLa cells. Tested complexes did not display genotoxic potential in human peripheral blood leucocytes, but exhibited an antigenotoxic effect in post-treatment, after H2O2 exposure. The study of the in vitro biological properties regarding their affinity towards CT (calf-thymus) DNA and serum albumins showed that the compounds can intercalate to CT DNA, and bind reversibly and tightly to the albumins. Molecular docking studies of the ability of compounds to bind to biomacromolecules are consistent with in vitro studies.",
publisher = "Elsevier Inc.",
journal = "Journal of Inorganic Biochemistry",
title = "Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins",
volume = "235",
doi = "10.1016/j.jinorgbio.2022.111942"
}
Vitomirov, T., Dimiza, F., Matić, I. Z., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Novaković, I., Anđelković, K., Milčić, M., Psomas, G.,& Šumar Ristović, M.. (2022). Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins. in Journal of Inorganic Biochemistry
Elsevier Inc.., 235.
https://doi.org/10.1016/j.jinorgbio.2022.111942
Vitomirov T, Dimiza F, Matić IZ, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Novaković I, Anđelković K, Milčić M, Psomas G, Šumar Ristović M. Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins. in Journal of Inorganic Biochemistry. 2022;235.
doi:10.1016/j.jinorgbio.2022.111942 .
Vitomirov, Teodora, Dimiza, Filitsa, Matić, Ivana Z., Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Novaković, Irena, Anđelković, Katarina, Milčić, Miloš, Psomas, George, Šumar Ristović, Maja, "Copper(II) complexes with 4-(diethylamino)salicylaldehyde and α-diimines: Anticancer, antioxidant, antigenotoxic effects and interaction with DNA and albumins" in Journal of Inorganic Biochemistry, 235 (2022),
https://doi.org/10.1016/j.jinorgbio.2022.111942 . .
7
7

Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro

Živković, Lada; Topalović, Dijana; Borozan, Sunčica; Bajić, Vladan; Dimitrijević Srećković, Vesna; Pirković, Andrea; Petrović, Hristina; Spremo-Potparević, Biljana

(Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands, 2022)

TY  - CONF
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Dimitrijević Srećković, Vesna
AU  - Pirković, Andrea
AU  - Petrović, Hristina
AU  - Spremo-Potparević, Biljana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4630
PB  - Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands
C3  - Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
T1  - Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro
SP  - 94
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4630
ER  - 
@conference{
author = "Živković, Lada and Topalović, Dijana and Borozan, Sunčica and Bajić, Vladan and Dimitrijević Srećković, Vesna and Pirković, Andrea and Petrović, Hristina and Spremo-Potparević, Biljana",
year = "2022",
publisher = "Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands",
journal = "Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book",
title = "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro",
pages = "94-94",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4630"
}
Živković, L., Topalović, D., Borozan, S., Bajić, V., Dimitrijević Srećković, V., Pirković, A., Petrović, H.,& Spremo-Potparević, B.. (2022). Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book
Department of Pharmacology and Toxicology, University of Maastrich, The Netherlands., 94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630
Živković L, Topalović D, Borozan S, Bajić V, Dimitrijević Srećković V, Pirković A, Petrović H, Spremo-Potparević B. Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro. in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book. 2022;:94-94.
https://hdl.handle.net/21.15107/rcub_farfar_4630 .
Živković, Lada, Topalović, Dijana, Borozan, Sunčica, Bajić, Vladan, Dimitrijević Srećković, Vesna, Pirković, Andrea, Petrović, Hristina, Spremo-Potparević, Biljana, "Dihydroquercetin (Taxifolin) and Biochaga attenuate oxidative DNA damage in lymphocytes of obese subjects in vitro" in Joint EEMGS meeting & International Comet Assay Workshop, 2022 - Abstract Book (2022):94-94,
https://hdl.handle.net/21.15107/rcub_farfar_4630 .

Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)

Mićović, Tijana; Topalović, Dijana; Živković, Lada; Spremo-Potparević, Biljana; Jakovljević, Vladimir; Matić, Sanja; Popović, Suzana; Baskić, Dejan; Stešević, Danijela; Samardžić, Stevan; Stojanović, Danilo; Maksimović, Zoran

(MDPI AG, 2021)

TY  - JOUR
AU  - Mićović, Tijana
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Jakovljević, Vladimir
AU  - Matić, Sanja
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Stešević, Danijela
AU  - Samardžić, Stevan
AU  - Stojanović, Danilo
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3817
AB  - Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.
PB  - MDPI AG
T2  - Plants
T1  - Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)
VL  - 10
IS  - 4
DO  - 10.3390/plants10040711
ER  - 
@article{
author = "Mićović, Tijana and Topalović, Dijana and Živković, Lada and Spremo-Potparević, Biljana and Jakovljević, Vladimir and Matić, Sanja and Popović, Suzana and Baskić, Dejan and Stešević, Danijela and Samardžić, Stevan and Stojanović, Danilo and Maksimović, Zoran",
year = "2021",
abstract = "Abstract:Hyssopus officinalisL. is a well-known aromatic plant used in traditional medicine and the foodand cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic andcytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical compositionwas analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diodearray detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examinedby 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxicand antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480,MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oilswere rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the mostabundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively).Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50= 56.04–199.89μg/mL,FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in humanwhole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selectiveand potent dose- and time-dependent activity against the HeLa cell line.  Results of the current studydemonstrated notableH.officinalismedicinal potential, which calls for further investigation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)",
volume = "10",
number = "4",
doi = "10.3390/plants10040711"
}
Mićović, T., Topalović, D., Živković, L., Spremo-Potparević, B., Jakovljević, V., Matić, S., Popović, S., Baskić, D., Stešević, D., Samardžić, S., Stojanović, D.,& Maksimović, Z.. (2021). Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants
MDPI AG., 10(4).
https://doi.org/10.3390/plants10040711
Mićović T, Topalović D, Živković L, Spremo-Potparević B, Jakovljević V, Matić S, Popović S, Baskić D, Stešević D, Samardžić S, Stojanović D, Maksimović Z. Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae). in Plants. 2021;10(4).
doi:10.3390/plants10040711 .
Mićović, Tijana, Topalović, Dijana, Živković, Lada, Spremo-Potparević, Biljana, Jakovljević, Vladimir, Matić, Sanja, Popović, Suzana, Baskić, Dejan, Stešević, Danijela, Samardžić, Stevan, Stojanović, Danilo, Maksimović, Zoran, "Antioxidant, antigenotoxic and cytotoxic activity of essential oils and methanol extracts of hyssopus officinalis l. Subsp. aristatus (godr.) nyman (lamiaceae)" in Plants, 10, no. 4 (2021),
https://doi.org/10.3390/plants10040711 . .
2
7
2
7

Olive leaf, DNA damage and chelation therapy

Čabarkapa-Pirković, Andrea; Živković, Lada; Dekanski, Dragana; Topalović, Dijana; Spremo-Potparević, Biljana

(Elsevier, 2021)

TY  - CHAP
AU  - Čabarkapa-Pirković, Andrea
AU  - Živković, Lada
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5184
AB  - Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.
PB  - Elsevier
T2  - Olives and Olive Oil in Health and Disease Prevention
T1  - Olive leaf, DNA damage and chelation therapy
SP  - 457
EP  - 469
DO  - 10.1016/B978-0-12-819528-4.00021-3
ER  - 
@inbook{
author = "Čabarkapa-Pirković, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Olive leaf (OL) is a precious source of bioactive compounds for versatile biomedical applications. Oleuropein, the main constituent from olive leaves, and its metabolite hydroxytyrosol are responsible for the majority of the observed biological effects. However, joined components of OL achieved better biological effects than its isolated individual constituents, indicating their synergistic effect. Predominantly free radical scavenging and metal chelation properties of OL compounds are responsible for the protective effects against oxidative damage and could be endorsed for its antioxidant and antigenotoxic effects. Local environment in cells such as the presence of transition metals, oxygen radicals, change in pH value, and the concentration of the flavonoid compounds can modify their activity from antioxidant to prooxidant and from antigenotoxic to genotoxic. Effects of complexes formed between flavonoids, metals, and DNA molecule, in heavy metal intoxicated individuals that are treated with ethylenediaminetetraacetic acid chelation therapy, require further research attention considering the demonstrated prooxidant and genotoxic effects.",
publisher = "Elsevier",
journal = "Olives and Olive Oil in Health and Disease Prevention",
booktitle = "Olive leaf, DNA damage and chelation therapy",
pages = "457-469",
doi = "10.1016/B978-0-12-819528-4.00021-3"
}
Čabarkapa-Pirković, A., Živković, L., Dekanski, D., Topalović, D.,& Spremo-Potparević, B.. (2021). Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention
Elsevier., 457-469.
https://doi.org/10.1016/B978-0-12-819528-4.00021-3
Čabarkapa-Pirković A, Živković L, Dekanski D, Topalović D, Spremo-Potparević B. Olive leaf, DNA damage and chelation therapy. in Olives and Olive Oil in Health and Disease Prevention. 2021;:457-469.
doi:10.1016/B978-0-12-819528-4.00021-3 .
Čabarkapa-Pirković, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Spremo-Potparević, Biljana, "Olive leaf, DNA damage and chelation therapy" in Olives and Olive Oil in Health and Disease Prevention (2021):457-469,
https://doi.org/10.1016/B978-0-12-819528-4.00021-3 . .
2
1

DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats

Bruić, Marija; Grujić-Milanović, Jelica; Miloradović, Zoran; Jovović, Đurđica; Živković, Lada; Mihailović-Stanojević, Nevena; Karanović, Danijela; Spremo-Potparević, Biljana

(Elsevier Ltd, 2021)

TY  - JOUR
AU  - Bruić, Marija
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Živković, Lada
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3981
AB  - Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.
PB  - Elsevier Ltd
T2  - International Journal of Biochemistry and Cell Biology
T1  - DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats
VL  - 141
DO  - 10.1016/j.biocel.2021.106088
ER  - 
@article{
author = "Bruić, Marija and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Živković, Lada and Mihailović-Stanojević, Nevena and Karanović, Danijela and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.",
publisher = "Elsevier Ltd",
journal = "International Journal of Biochemistry and Cell Biology",
title = "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats",
volume = "141",
doi = "10.1016/j.biocel.2021.106088"
}
Bruić, M., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Živković, L., Mihailović-Stanojević, N., Karanović, D.,& Spremo-Potparević, B.. (2021). DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in International Journal of Biochemistry and Cell Biology
Elsevier Ltd., 141.
https://doi.org/10.1016/j.biocel.2021.106088
Bruić M, Grujić-Milanović J, Miloradović Z, Jovović Đ, Živković L, Mihailović-Stanojević N, Karanović D, Spremo-Potparević B. DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in International Journal of Biochemistry and Cell Biology. 2021;141.
doi:10.1016/j.biocel.2021.106088 .
Bruić, Marija, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Živković, Lada, Mihailović-Stanojević, Nevena, Karanović, Danijela, Spremo-Potparević, Biljana, "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats" in International Journal of Biochemistry and Cell Biology, 141 (2021),
https://doi.org/10.1016/j.biocel.2021.106088 . .
9
6

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Monica, Neri; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine A. S.; Teixeira, João Paulo; Bruzzone, , Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo’, Cristian; Russo, Patrizia; Dobrzyńska, , Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina I.; Staruchova, Marta; Barančokova, Magdalena; Volkovova, Katarina; Kažimirova, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernández, Alba; Gajski, Goran; Spremo-Potparević, Biljana; Živković, Lada; Boutet-Robinet, Elisa; Perdry, Hervé; Lebailly, Pierre; Perez, Carlos L.; Basaran, Nursen; Nemeth, , Zsuzsanna; Safar, Anna; Dusinska, Maria; Collins, Andrew; Anderson, Diana; Andrade, Vanessa; Pereira, Cristiana Costa; Costa, Solange; Gutzkow, Kristine B.; Ladeira, Carina; Moretti, Massimo; Costa, Carla; Orlow, Irene; Rojas, Emilio; Pourrut, Bertrand; Kruszewski, Marcin; Knasmueller, Siegfried; Shaposhnikov, Sergey; Žegura, Bojana; Stopper, Helga

(Nature Research, 2021)

TY  - JOUR
AU  - Bonassi, Stefano
AU  - Ceppi, Marcello
AU  - Møller, Peter
AU  - Azqueta, Amaya
AU  - Milić, Mirta
AU  - Monica, Neri
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine A. S.
AU  - Teixeira, João Paulo
AU  - Bruzzone, , Marco
AU  - Da Silva, Juliana
AU  - Benedetti, Danieli
AU  - Cavallo, Delia
AU  - Ursini, Cinzia Lucia
AU  - Giovannelli, Lisa
AU  - Moretti, Silvia
AU  - Riso, Patrizia
AU  - Del Bo’, Cristian
AU  - Russo, Patrizia
AU  - Dobrzyńska, , Malgorzata
AU  - Goroshinskaya, Irina A.
AU  - Surikova, Ekaterina I.
AU  - Staruchova, Marta
AU  - Barančokova, Magdalena
AU  - Volkovova, Katarina
AU  - Kažimirova, Alena
AU  - Smolkova, Bozena
AU  - Laffon, Blanca
AU  - Valdiglesias, Vanessa
AU  - Pastor, Susana
AU  - Marcos, Ricard
AU  - Hernández, Alba
AU  - Gajski, Goran
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Boutet-Robinet, Elisa
AU  - Perdry, Hervé
AU  - Lebailly, Pierre
AU  - Perez, Carlos L.
AU  - Basaran, Nursen
AU  - Nemeth, , Zsuzsanna
AU  - Safar, Anna
AU  - Dusinska, Maria
AU  - Collins, Andrew
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Pereira, Cristiana Costa
AU  - Costa, Solange
AU  - Gutzkow, Kristine B.
AU  - Ladeira, Carina
AU  - Moretti, Massimo
AU  - Costa, Carla
AU  - Orlow, Irene
AU  - Rojas, Emilio
AU  - Pourrut, Bertrand
AU  - Kruszewski, Marcin
AU  - Knasmueller, Siegfried
AU  - Shaposhnikov, Sergey
AU  - Žegura, Bojana
AU  - Stopper, Helga
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3944
AB  - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
PB  - Nature Research
T2  - Scientific Reports
T1  - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
VL  - 11
IS  - 1
DO  - 10.1038/s41598-021-95976-7
ER  - 
@article{
author = "Bonassi, Stefano and Ceppi, Marcello and Møller, Peter and Azqueta, Amaya and Milić, Mirta and Monica, Neri and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine A. S. and Teixeira, João Paulo and Bruzzone, , Marco and Da Silva, Juliana and Benedetti, Danieli and Cavallo, Delia and Ursini, Cinzia Lucia and Giovannelli, Lisa and Moretti, Silvia and Riso, Patrizia and Del Bo’, Cristian and Russo, Patrizia and Dobrzyńska, , Malgorzata and Goroshinskaya, Irina A. and Surikova, Ekaterina I. and Staruchova, Marta and Barančokova, Magdalena and Volkovova, Katarina and Kažimirova, Alena and Smolkova, Bozena and Laffon, Blanca and Valdiglesias, Vanessa and Pastor, Susana and Marcos, Ricard and Hernández, Alba and Gajski, Goran and Spremo-Potparević, Biljana and Živković, Lada and Boutet-Robinet, Elisa and Perdry, Hervé and Lebailly, Pierre and Perez, Carlos L. and Basaran, Nursen and Nemeth, , Zsuzsanna and Safar, Anna and Dusinska, Maria and Collins, Andrew and Anderson, Diana and Andrade, Vanessa and Pereira, Cristiana Costa and Costa, Solange and Gutzkow, Kristine B. and Ladeira, Carina and Moretti, Massimo and Costa, Carla and Orlow, Irene and Rojas, Emilio and Pourrut, Bertrand and Kruszewski, Marcin and Knasmueller, Siegfried and Shaposhnikov, Sergey and Žegura, Bojana and Stopper, Helga",
year = "2021",
abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death",
volume = "11",
number = "1",
doi = "10.1038/s41598-021-95976-7"
}
Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milić, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, ,. M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., Del Bo’, C., Russo, P., Dobrzyńska, ,. M., Goroshinskaya, I. A., Surikova, E. I., Staruchova, M., Barančokova, M., Volkovova, K., Kažimirova, A., Smolkova, B., Laffon, B., Valdiglesias, V., Pastor, S., Marcos, R., Hernández, A., Gajski, G., Spremo-Potparević, B., Živković, L., Boutet-Robinet, E., Perdry, H., Lebailly, P., Perez, C. L., Basaran, N., Nemeth, ,. Z., Safar, A., Dusinska, M., Collins, A., Anderson, D., Andrade, V., Pereira, C. C., Costa, S., Gutzkow, K. B., Ladeira, C., Moretti, M., Costa, C., Orlow, I., Rojas, E., Pourrut, B., Kruszewski, M., Knasmueller, S., Shaposhnikov, S., Žegura, B.,& Stopper, H.. (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports
Nature Research., 11(1).
https://doi.org/10.1038/s41598-021-95976-7
Bonassi S, Ceppi M, Møller P, Azqueta A, Milić M, Monica N, Brunborg G, Godschalk R, Koppen G, Langie SAS, Teixeira JP, Bruzzone ,M, Da Silva J, Benedetti D, Cavallo D, Ursini CL, Giovannelli L, Moretti S, Riso P, Del Bo’ C, Russo P, Dobrzyńska ,M, Goroshinskaya IA, Surikova EI, Staruchova M, Barančokova M, Volkovova K, Kažimirova A, Smolkova B, Laffon B, Valdiglesias V, Pastor S, Marcos R, Hernández A, Gajski G, Spremo-Potparević B, Živković L, Boutet-Robinet E, Perdry H, Lebailly P, Perez CL, Basaran N, Nemeth ,Z, Safar A, Dusinska M, Collins A, Anderson D, Andrade V, Pereira CC, Costa S, Gutzkow KB, Ladeira C, Moretti M, Costa C, Orlow I, Rojas E, Pourrut B, Kruszewski M, Knasmueller S, Shaposhnikov S, Žegura B, Stopper H. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-95976-7 .
Bonassi, Stefano, Ceppi, Marcello, Møller, Peter, Azqueta, Amaya, Milić, Mirta, Monica, Neri, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, , Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska, , Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević, Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran, Nursen, Nemeth, , Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins, Andrew, Anderson, Diana, Andrade, Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, Stopper, Helga, "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-95976-7 . .
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Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes

Spremo-Potparević, Biljana; Čabarkapa-Pirković, Andrea; Dekanski, Dragana; Topalović, Dijana; Bajić, Vladan; Živković, Lada

(Beograd : Savez farmaceutskih udruženja Srbije, 2021)

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920
AB  - Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
AB  - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
T1  - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
VL  - 71
IS  - 3
SP  - 197
EP  - 206
DO  - 10.5937/arhfarm71‐31060
ER  - 
@article{
author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada",
year = "2021",
abstract = "Strawberry  fruit Fragaria  ×  ananassa  Duchesne,  Rosaceae  (cv.  Romina),  rich  in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka",
volume = "71",
number = "3",
pages = "197-206",
doi = "10.5937/arhfarm71‐31060"
}
Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206.
https://doi.org/10.5937/arhfarm71‐31060
Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206.
doi:10.5937/arhfarm71‐31060 .
Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206,
https://doi.org/10.5937/arhfarm71‐31060 . .

The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders

Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, Peter; Teixeira, João Paulo; Alija, Avdulla; Anderson, Diana; Andrade, Vanessa; Andreoli, Cristina; Asllani, Fisnik; Bangkoglu, Ezgi Eyluel; Barančoková, Magdalena; Basaran, Nursen; Boutet-Robinet, Elisa; Buschini, Annamaria; Cavallo, Delia; Costa Pereira, Cristiana; Costa, Carla; Costa, Solange; Da Silva, Juliana; Del Boˊ, Cristian; Dimitrijević Srećković, Vesna; Đelić, Ninoslav; Dobrzyńska, Malgorzata; Duračková, Zdenka; Dvořáková, Monika; Gajski, Goran; Galati, Serena; García Lima, Omar; Giovannelli, Lisa; Goroshinskaya, Irina A.; Grindel, Annemarie; Gutzkow, Kristine B.; Hernández, Alba; Hernández, Carlos; Holven, Kirsten B.; Ibero-Baraibar, Idoia; Ottestad, Inger; Kadioglu, Ela; Kažimirová, Alena; Kuznetsova, Elena; Ladeira, Carina; Laffon, Blanca; Lamonaca, Palma; Lebailly, Pierre; Louro, Henriqueta; Mandina Cardoso, Tania; Marcon, Francesca; Marcos, Ricard; Moretti, Massimo; Moretti, Silvia; Najafzadeh, Mojgan; Nemeth, Zsuzsanna; Neri, Monica; Novotna, Bozena; Orlow, Irene; Paduchova, Zuzana; Pastor, Susana; Perdry, Hervé; Spremo-Potparević, Biljana; Ramadhani, Dwi; Riso, Patrizia; Rohr, Paula; Rojas, Emilio; Rossner, Pavel; Safar, Anna; Sardas, Semra; Silva, Maria João; Sirota, Nikolay; Smolkova, Bozena; Staruchova, Marta; Stetina, Rudolf; Stopper, Helga; Surikova, Ekaterina I.; Ulven, Stine M.; Ursini, Cinzia Lucia; Valdiglesias, Vanessa; Valverde, Mahara; Vodicka, Pavel; Volkovova, Katarina; Wagner, Karl-Heinz; Živković, Lada; Dušinská, Maria; Collins, Andrew R.; Bonassi, Stefano

(Elsevier B.V., 2021)

TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3791
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier B.V.
T2  - Mutation Research - Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
DO  - 10.1016/j.mrrev.2021.108371
ER  - 
@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
doi = "10.1016/j.mrrev.2021.108371"
}
Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research
Elsevier B.V.., 787.
https://doi.org/10.1016/j.mrrev.2021.108371
Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research. 2021;787.
doi:10.1016/j.mrrev.2021.108371 .
Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research - Reviews in Mutation Research, 787 (2021),
https://doi.org/10.1016/j.mrrev.2021.108371 . .
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44

Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease

Živković, Lada; Bajić, Vladan; Čabarkapa-Pirković, Andrea; Dekanski, Dragana; Forbes-Hernández, Tamara; Zlatković-Švenda, Mirjana; Perry, George; Spremo-Potparević, Biljana

(Wiley, 2021)

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes-Hernández, Tamara
AU  - Zlatković-Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
PB  - Wiley
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.",
publisher = "Wiley",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}
Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry
Wiley., 45(4).
https://doi.org/10.1111/jfbc.13637
Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .
Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .
13
2
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2

Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7)

Bonassi, Stefano; Ceppi,Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Neri, Monica; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine A. S.; Teixeira, João Paulo; Bruzzone, Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo’, Cristian; Russo, Patrizia; Dobrzyńska, Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina I.; Staruchova, Marta; Barančokova, Magdalena; Volkovova, Katarina; Kažimirova, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernández, Alba; Gajski, Goran; Spremo-Potparević, Biljana; Živković, Lada; Boutet-Robinet, Elisa; Perdry, Hervé; Lebailly, Pierre; Perez, Carlos L.; Basaran, Nursen; Nemeth, Zsuzsanna; Safar, Anna; Dusinska, Maria; Collins, Andrew; Anderson, Diana; Andrade, Vanessa; Pereira, Cristiana Costa; Costa, Solange; Gutzkow, Kristine B.; Ladeira, Carina; Moretti, Massimo; Costa, Carla; Orlow, Irene; Rojas, Emilio; Pourrut, Bertrand; Kruszewski, Marcin; Knasmueller, Siegfried; Shaposhnikov, Sergey; Žegura, Bojana; Stopper, Helga

(Nature Research, 2021)

TY  - JOUR
AU  - Bonassi, Stefano
AU  - Ceppi,Marcello
AU  - Møller,  Peter
AU  - Azqueta, Amaya
AU  - Milić, Mirta
AU  - Neri, Monica
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine A. S.
AU  - Teixeira, João Paulo
AU  - Bruzzone, Marco
AU  - Da Silva, Juliana
AU  - Benedetti, Danieli
AU  - Cavallo, Delia
AU  - Ursini, Cinzia Lucia
AU  - Giovannelli, Lisa
AU  - Moretti, Silvia
AU  - Riso, Patrizia
AU  - Del Bo’, Cristian
AU  - Russo, Patrizia
AU  - Dobrzyńska,  Malgorzata
AU  - Goroshinskaya, Irina A.
AU  - Surikova, Ekaterina I.
AU  - Staruchova, Marta
AU  - Barančokova, Magdalena
AU  - Volkovova, Katarina
AU  - Kažimirova, Alena
AU  - Smolkova, Bozena
AU  - Laffon, Blanca
AU  - Valdiglesias, Vanessa
AU  - Pastor, Susana
AU  - Marcos, Ricard
AU  - Hernández, Alba
AU  - Gajski, Goran
AU  - Spremo-Potparević,  Biljana
AU  - Živković, Lada
AU  - Boutet-Robinet, Elisa
AU  - Perdry, Hervé
AU  - Lebailly, Pierre
AU  - Perez, Carlos L.
AU  - Basaran,  Nursen
AU  - Nemeth, Zsuzsanna
AU  - Safar, Anna
AU  - Dusinska, Maria
AU  - Collins,  Andrew
AU  - Anderson, Diana
AU  - Andrade,  Vanessa
AU  - Pereira, Cristiana Costa
AU  - Costa, Solange
AU  - Gutzkow, Kristine B.
AU  - Ladeira, Carina
AU  - Moretti, Massimo
AU  - Costa, Carla
AU  - Orlow, Irene
AU  - Rojas, Emilio
AU  - Pourrut, Bertrand
AU  - Kruszewski, Marcin
AU  - Knasmueller, Siegfried
AU  - Shaposhnikov, Sergey
AU  - Žegura, Bojana
AU  - Stopper, Helga
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3975
PB  - Nature Research
T2  - Scientific Reports
T1  - Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7)
VL  - 11
DO  - 10.1038/s41598-021-98620-6
ER  - 
@article{
author = "Bonassi, Stefano and Ceppi,Marcello and Møller,  Peter and Azqueta, Amaya and Milić, Mirta and Neri, Monica and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine A. S. and Teixeira, João Paulo and Bruzzone, Marco and Da Silva, Juliana and Benedetti, Danieli and Cavallo, Delia and Ursini, Cinzia Lucia and Giovannelli, Lisa and Moretti, Silvia and Riso, Patrizia and Del Bo’, Cristian and Russo, Patrizia and Dobrzyńska,  Malgorzata and Goroshinskaya, Irina A. and Surikova, Ekaterina I. and Staruchova, Marta and Barančokova, Magdalena and Volkovova, Katarina and Kažimirova, Alena and Smolkova, Bozena and Laffon, Blanca and Valdiglesias, Vanessa and Pastor, Susana and Marcos, Ricard and Hernández, Alba and Gajski, Goran and Spremo-Potparević,  Biljana and Živković, Lada and Boutet-Robinet, Elisa and Perdry, Hervé and Lebailly, Pierre and Perez, Carlos L. and Basaran,  Nursen and Nemeth, Zsuzsanna and Safar, Anna and Dusinska, Maria and Collins,  Andrew and Anderson, Diana and Andrade,  Vanessa and Pereira, Cristiana Costa and Costa, Solange and Gutzkow, Kristine B. and Ladeira, Carina and Moretti, Massimo and Costa, Carla and Orlow, Irene and Rojas, Emilio and Pourrut, Bertrand and Kruszewski, Marcin and Knasmueller, Siegfried and Shaposhnikov, Sergey and Žegura, Bojana and Stopper, Helga",
year = "2021",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7)",
volume = "11",
doi = "10.1038/s41598-021-98620-6"
}
Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milić, M., Neri, M., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., Del Bo’, C., Russo, P., Dobrzyńska, M., Goroshinskaya, I. A., Surikova, E. I., Staruchova, M., Barančokova, M., Volkovova, K., Kažimirova, A., Smolkova, B., Laffon, B., Valdiglesias, V., Pastor, S., Marcos, R., Hernández, A., Gajski, G., Spremo-Potparević, B., Živković, L., Boutet-Robinet, E., Perdry, H., Lebailly, P., Perez, C. L., Basaran, N., Nemeth, Z., Safar, A., Dusinska, M., Collins, A., Anderson, D., Andrade, V., Pereira, C. C., Costa, S., Gutzkow, K. B., Ladeira, C., Moretti, M., Costa, C., Orlow, I., Rojas, E., Pourrut, B., Kruszewski, M., Knasmueller, S., Shaposhnikov, S., Žegura, B.,& Stopper, H.. (2021). Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7). in Scientific Reports
Nature Research., 11.
https://doi.org/10.1038/s41598-021-98620-6
Bonassi S, Ceppi M, Møller P, Azqueta A, Milić M, Neri M, Brunborg G, Godschalk R, Koppen G, Langie SAS, Teixeira JP, Bruzzone M, Da Silva J, Benedetti D, Cavallo D, Ursini CL, Giovannelli L, Moretti S, Riso P, Del Bo’ C, Russo P, Dobrzyńska M, Goroshinskaya IA, Surikova EI, Staruchova M, Barančokova M, Volkovova K, Kažimirova A, Smolkova B, Laffon B, Valdiglesias V, Pastor S, Marcos R, Hernández A, Gajski G, Spremo-Potparević B, Živković L, Boutet-Robinet E, Perdry H, Lebailly P, Perez CL, Basaran N, Nemeth Z, Safar A, Dusinska M, Collins A, Anderson D, Andrade V, Pereira CC, Costa S, Gutzkow KB, Ladeira C, Moretti M, Costa C, Orlow I, Rojas E, Pourrut B, Kruszewski M, Knasmueller S, Shaposhnikov S, Žegura B, Stopper H. Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7). in Scientific Reports. 2021;11.
doi:10.1038/s41598-021-98620-6 .
Bonassi, Stefano, Ceppi,Marcello, Møller,  Peter, Azqueta, Amaya, Milić, Mirta, Neri, Monica, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska,  Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević,  Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran,  Nursen, Nemeth, Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins,  Andrew, Anderson, Diana, Andrade,  Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, Stopper, Helga, "Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death (Scientific Reports, (2021), 11, 1, (16793), 10.1038/s41598-021-95976-7)" in Scientific Reports, 11 (2021),
https://doi.org/10.1038/s41598-021-98620-6 . .
1

Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac

Pirković-Čabarkapa, Andrea; Živković, Lada; Zlatković-Švenda, Mirjana; Borozan, Sunčica; Topalović, Dijana; Dekanski, Dragana; Bruić, Marija; Bajić, Vladan; Radak-Perović, Marija; Spremo-Potparević, Biljana

(Serbian Genetics Society, 2020)

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 
@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}
Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P
Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .
Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .
1

Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay

Topalović, Dijana; Živković, Lada; Đelić, Ninoslav; Bajić, Vladan; Spremo-Potparević, Biljana

(Serbian Medical Society, 2020)

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3900
AB  - Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay
T1  - Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 
@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5’-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1 µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5'-monofosfat dehidrogenaze (IMPDH) povec'ana je u c'elijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđeno da je izloženost tiazofurinu pod određenim okolnostima imala vec'u incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoc'u komet testa ispita genotoksično delovanje i izazivanje DNK oštec'enja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštec'enje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1 µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštec'enja na c'elijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje c'elija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštec'enja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay, Analiza DNK oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}
Topalović, D., Živković, L., Đelić, N., Bajić, V.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis
Serbian Medical Society., 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798
Topalović D, Živković L, Đelić N, Bajić V, Spremo-Potparević B. Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay. in Medicinski Casopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .
Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced dna damage in human whole blood cells using an in vitro comet assay" in Medicinski Casopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”

Bajić, Vladan. P.; Essack, Magbubah; Živković, Lada; Stewart, Alan; Zafirović, Sonja; Bajić, Vladimir B.; Gojobori, Takashi; Isenović, Esma; Spremo-Potparević, Biljana

(Frontiers Media S.A., 2020)

TY  - JOUR
AU  - Bajić, Vladan. P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3554
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.
PB  - Frontiers Media S.A.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 
@article{
author = "Bajić, Vladan. P. and Essack, Magbubah and Živković, Lada and Stewart, Alan and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}
Bajić, Vladan. P., Essack, M., Živković, L., Stewart, A., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E.,& Spremo-Potparević, B.. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fgene.2019.01368
Bajić VP, Essack M, Živković L, Stewart A, Zafirović S, Bajić VB, Gojobori T, Isenović E, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics. 2020;10.
doi:10.3389/fgene.2019.01368 .
Bajić, Vladan. P., Essack, Magbubah, Živković, Lada, Stewart, Alan, Zafirović, Sonja, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma, Spremo-Potparević, Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" in Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 . .
13
24
7
23

Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy

Obradović, Milan; Zafirović, Sonja; Essack, Magbubah; Dimitrov, Jelena; Živković, Lada; Spremo-Potparević, Biljana; Radak, Đorđe; Bajić, Vladimir B.; Isenović, Esma

(Elsevier, 2020)

TY  - JOUR
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Dimitrov, Jelena
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Radak, Đorđe
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3462
AB  - To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes.
PB  - Elsevier
T2  - Medical Hypotheses
T1  - Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy
VL  - 134
SP  - 1
EP  - 5
DO  - 10.1016/j.mehy.2019.109419
ER  - 
@article{
author = "Obradović, Milan and Zafirović, Sonja and Essack, Magbubah and Dimitrov, Jelena and Živković, Lada and Spremo-Potparević, Biljana and Radak, Đorđe and Bajić, Vladimir B. and Isenović, Esma",
year = "2020",
abstract = "To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes.",
publisher = "Elsevier",
journal = "Medical Hypotheses",
title = "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy",
volume = "134",
pages = "1-5",
doi = "10.1016/j.mehy.2019.109419"
}
Obradović, M., Zafirović, S., Essack, M., Dimitrov, J., Živković, L., Spremo-Potparević, B., Radak, Đ., Bajić, V. B.,& Isenović, E.. (2020). Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses
Elsevier., 134, 1-5.
https://doi.org/10.1016/j.mehy.2019.109419
Obradović M, Zafirović S, Essack M, Dimitrov J, Živković L, Spremo-Potparević B, Radak Đ, Bajić VB, Isenović E. Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses. 2020;134:1-5.
doi:10.1016/j.mehy.2019.109419 .
Obradović, Milan, Zafirović, Sonja, Essack, Magbubah, Dimitrov, Jelena, Živković, Lada, Spremo-Potparević, Biljana, Radak, Đorđe, Bajić, Vladimir B., Isenović, Esma, "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy" in Medical Hypotheses, 134 (2020):1-5,
https://doi.org/10.1016/j.mehy.2019.109419 . .
1
1
1
2

Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro

Topalović, Dijana; Dekanski, Dragana; Potparević, Biljana; Pirković, Andrea; Borozan, Sunčica; Bajić, Vladan; Stojanović, Danilo; Giampieri, Francesca; Gasparrini, Massimiliano; Živković, Lada

(Elsevier B.V., 2019)

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 
@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}
Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001
Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .
Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
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Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC

Živković, Lada; Bajić, Vladan; Topalović, Dijana; Bruić, Marija; Spremo-Potparević, Biljana

(Turkish Society of Toxicology, 2019)

TY  - CONF
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5187
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Turkish Society of Toxicology
C3  - 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
T1  - Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5187
ER  - 
@conference{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Turkish Society of Toxicology",
journal = "10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book",
title = "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5187"
}
Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book
Turkish Society of Toxicology., 169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187
Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5187 .
Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC" in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book (2019):169-169,
https://hdl.handle.net/21.15107/rcub_farfar_5187 .