Kovačević, Nada

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Authority KeyName Variants
orcid::0000-0002-4296-9974
  • Kovačević, Nada (113)
Projects
Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200161 (University of Belgrade, Faculty of Pharmacy) Preventive, therapeutic, and ethical approach in preclinical and clinical studies of the genes and modulators of redox cell signaling in immune, inflammatory and proliferative cell response
Plant Biodiversity of Serbia and the Balkans - assesment, sustainable use and protection Diversity of the flora and vegetation of the Central Balkans: Ecology, chorology, and conservation
Faculty of Medicine University of Niš Internal Scientific Project No. 15 Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200113 (Univeristy of Niš, Faculty of Medicine)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200124 (Univeristy of Niš, Faculty of Science) Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety
Investigation of contemporary biotechnological processes in animal feed production aimed at increasing food competitiveness, quality and safety Traditional and new products of cultivated and wild growing fruits and grape vines, and by-products durring processing, with special emphasis on indigenous varieties: chemical characterization and biological profile
Istraživanje dejstava modifikatora biološkog odgovora u fiziološkim i patološkim stanjima Morfološko-eksperimentalna i molekularno-biološka ispitivanja biliopankreasnog sistema relevantna za endoskopsku dijagnostiku i terapiju hroničnog pankreatitisa
Plants and synthetic bioactive products of new generation "Ispitivanje lekovitog potencijala samoniklih biljaka: morfološka, hemijska i farmakološka karakterizacija" (P1568), Ministarstva za nauku, tehnologiju i razvoj Republike Srbije
Ministry of Science and Technology of Republic of Srpska - 19/6-020/961-110/14

Author's Bibliography

Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)

Marčetić, Mirjana; Ilić, Đorđe; Slavkovska, Violeta; Kovačević, Nada

(Ботаничко друштво „Андреас Волни”, Нови Сад, 2023)

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Ilić, Đorđe
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5141
AB  - Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ...
AB  - The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)
T1  - Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)
SP  - 14
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5141
ER  - 
@conference{
author = "Marčetić, Mirjana and Ilić, Đorđe and Slavkovska, Violeta and Kovačević, Nada",
year = "2023",
abstract = "Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ..., The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae), Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)",
pages = "14-15",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5141"
}
Marčetić, M., Ilić, Đ., Slavkovska, V.,& Kovačević, N.. (2023). Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141
Marčetić M, Ilić Đ, Slavkovska V, Kovačević N. Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141 .
Marčetić, Mirjana, Ilić, Đorđe, Slavkovska, Violeta, Kovačević, Nada, "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):14-15,
https://hdl.handle.net/21.15107/rcub_farfar_5141 .

Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)

Arsenijević, Jelena; Slavkovska, Violeta; Drobac, Milica; Stojanović, Danilo; Zbiljić, Miloš; Kovačević, Nada

(Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 2022)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Slavkovska, Violeta
AU  - Drobac, Milica
AU  - Stojanović, Danilo
AU  - Zbiljić, Miloš
AU  - Kovačević, Nada
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4621
AB  - Satureja kitaibelii Wierzb. ex Heuff. (Rtanj tea) has distinct morphology and distribution which separate it from highly polymorphic S. montana L. In this work, we investigated phenolic composition of S. kitaibelii and compared it to that of S. montana. Flowering aerial parts of four S. kitaibelii samples were collected in Serbia and Bulgaria and one commercial sample of Rtanj tea was obtained from Serbia. Three samples of S. montana were collected in North Macedonia and Serbia and the fourth one was cultivated sample. Herbs were extracted with 50% ethanol. Dry extract solutions were analyzed by liquid chromatography with UV and MS detection. Rosmarinic acid (RA) and clinopodic acid O (CAO) were quantified using RA as the external standard. All extracts were characterized by presence of phenolic acids, RA (16.5-47.9 mg/g) and CAO (1.6-29.3 mg/g, calculated as RA), and flavonoids. The extracts of S. kitaibelii distinguished by high contents of both RA and CAO, which were present in similar quantities within each extract. On the other hand, extracts of S. montana contained at least 3 times less CAO than RA. The obtained results further confirm distinct properties of S. kitaibelii and give additional arguments to its status as a separate species.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)
SP  - 174
EP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4621
ER  - 
@conference{
author = "Arsenijević, Jelena and Slavkovska, Violeta and Drobac, Milica and Stojanović, Danilo and Zbiljić, Miloš and Kovačević, Nada",
year = "2022",
abstract = "Satureja kitaibelii Wierzb. ex Heuff. (Rtanj tea) has distinct morphology and distribution which separate it from highly polymorphic S. montana L. In this work, we investigated phenolic composition of S. kitaibelii and compared it to that of S. montana. Flowering aerial parts of four S. kitaibelii samples were collected in Serbia and Bulgaria and one commercial sample of Rtanj tea was obtained from Serbia. Three samples of S. montana were collected in North Macedonia and Serbia and the fourth one was cultivated sample. Herbs were extracted with 50% ethanol. Dry extract solutions were analyzed by liquid chromatography with UV and MS detection. Rosmarinic acid (RA) and clinopodic acid O (CAO) were quantified using RA as the external standard. All extracts were characterized by presence of phenolic acids, RA (16.5-47.9 mg/g) and CAO (1.6-29.3 mg/g, calculated as RA), and flavonoids. The extracts of S. kitaibelii distinguished by high contents of both RA and CAO, which were present in similar quantities within each extract. On the other hand, extracts of S. montana contained at least 3 times less CAO than RA. The obtained results further confirm distinct properties of S. kitaibelii and give additional arguments to its status as a separate species.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4621"
}
Arsenijević, J., Slavkovska, V., Drobac, M., Stojanović, D., Zbiljić, M.,& Kovačević, N.. (2022). Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae). in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 174-174.
https://hdl.handle.net/21.15107/rcub_farfar_4621
Arsenijević J, Slavkovska V, Drobac M, Stojanović D, Zbiljić M, Kovačević N. Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae). in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:174-174.
https://hdl.handle.net/21.15107/rcub_farfar_4621 .
Arsenijević, Jelena, Slavkovska, Violeta, Drobac, Milica, Stojanović, Danilo, Zbiljić, Miloš, Kovačević, Nada, "Comparison of phenolic profiles of Satureja kitaibelii Wierzb. ex Heuff. and Satureja montana L. (Lamiaceae)" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):174-174,
https://hdl.handle.net/21.15107/rcub_farfar_4621 .

Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)

Arsenijević, Jelena; Drobac, Milica; Slavkovska, Violeta; Dabetić, Nevena; Marcic, Christophe; Kovačević, Nada; Ražić, Slavica

(Savez farmaceutskih udruženja Srbije (SFUS), 2022)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Dabetić, Nevena
AU  - Marcic, Christophe
AU  - Kovačević, Nada
AU  - Ražić, Slavica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4587
AB  - Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA
oligomers, are important active constituents of Lamiaceae plants. It was found that herb of
Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer
clinopodic acid O (CAO) (1).
In this work, we compared extracting efficiencies of conventional solvents and a deep
eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of
RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication
(room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol
96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C,
120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external
standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and
CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of
CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol
(925 μg/mL). These extracts contained significantly more of RA and CAO than the extract
obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96%
ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10
μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj
tea, for purpose of both quality control and preparation of extracts with potential application
in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and
“green pharmacy” principles.
AB  - Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina
(RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je
ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i
heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona
efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i
limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak
rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60
min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom,
kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1).
Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda.
Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO,
pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je
za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925
μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog
pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane,
ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217
μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke
smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu
ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u
skladu sa principima “zelene hemije” i “zelene farmacije”.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
T1  - Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)
VL  - 72
IS  - 4 suplement
SP  - S481
EP  - S482
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4587
ER  - 
@conference{
author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Dabetić, Nevena and Marcic, Christophe and Kovačević, Nada and Ražić, Slavica",
year = "2022",
abstract = "Hydroxycinnamic acids (HCAs), such are dimeric rosmarinic acid (RA) and higher HCA
oligomers, are important active constituents of Lamiaceae plants. It was found that herb of
Satureja kitaibelii (Rtanj tea) contains substantial amounts of RA and HCA hexamer
clinopodic acid O (CAO) (1).
In this work, we compared extracting efficiencies of conventional solvents and a deep
eutectic solvent, composed of choline chloride and citric acid (ChCl:CitrA), for extraction of
RA and CAO from Rtanj tea. Commercial Rtanj tea sample was extracted (1:10) by sonication
(room temperature, 60 min) with aqueous ChCl:CitrA (35% and 65%), ethanol 50%, ethanol
96%, and water, as well as by previously optimized procedure (ethanol 46%, digestion 60 °C,
120 min) (1). Contents of RA and CAO were determined by HPLC using RA as external
standard. Extraction with ChCl:CitrA and 50% ethanol resulted in extracts rich in RA and
CAO; RA concentrations in these extracts were uniform (848-873 μg/mL), while extraction of
CAO was slightly more effective with ChCl:CitrA (1122-1131 μg/mL) than with 50% ethanol
(925 μg/mL). These extracts contained significantly more of RA and CAO than the extract
obtained under optimized conditions (666 μg/mL RA, 725 μg/mL CAO). Differently, 96%
ethanol and water extracts contained substantially less RA (11-217 μg/mL) and CAO (4-10
μg/mL). Obtained results indicate potential of applied eutectic solvent in extraction of Rtanj
tea, for purpose of both quality control and preparation of extracts with potential application
in pharmaceutical, food or cosmetic industry, and are compliant with “green chemistry” and
“green pharmacy” principles., Derivati hidroksicimetne kiseline (HCK), kao što su dimerna rozmarinska kiselina
(RK) i viši oligomeri HCK, su aktivni sastojci biljaka familije Lamiaceae. Ranije je
ustanovljeno da herba Satureja kitaibelii (rtanjski čaj) sadrži značajne količine RK i
heksamera HCK klinopodinske kiseline O (KKO) (1). U radu je upoređena ekstrakciona
efikasnost konvencionalnih rastvarača i duboke eutektičke smeše na bazi holin hlorida i
limunske kiseline (ChCl:CitrA) za ekstrakciju RK i KKO iz rtanjskog čaja. Komercijalni uzorak
rtanjskog čaja ekstrahovan je (1:10) postupkom sonikacije na sobnoj temperaturi tokom 60
min vodenim rastvorima ChCl:CitrA (35% i 65%), etanolom 50%, etanolom 96% i vodom,
kao i prethodno optimizovanim postupkom (etanol 46%, digestija na 60 °C, 120 min) (1).
Sadržaj RK i KKO određen je HPLC metodom, korišćenjem RK kao eksternog standarda.
Ekstrakcija primenom ChCl:CitrA i 50% etanola rezultirala je ekstraktima bogatim RK i KKO,
pri čemu su koncentracije RK u ovim ekstraktima bile ujednačene (848-873 μg/mL), dok je
za ekstrakciju KKO, ChCl:CitrA bila nešto efikasnija (1122-1131 μg/mL) od 50% etanola (925
μg/mL). Pored toga, ovi ekstrakti su sadržali veće količine RK i KKO od ekstrakta dobijenog
pod ranije optimizovanim uslovima (666 μg/mL RK, 725 μg/mL KKO). Sa druge strane,
ekstrakti dobijeni 96% etanolom i vodom sadržali su znatno manje količine RK (11-217
μg/mL) i KKO (4-10 μg/mL). Dobijeni rezultati ukazuju na potencijal primenjene eutektičke
smeše u ekstrakciji rtanjskog čaja, kako u cilju kontrole kvaliteta, tako i za pripremu
ekstrakata sa primenom u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, što je u
skladu sa principima “zelene hemije” i “zelene farmacije”.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae), Poređenje efikasnosti eutektičke smeše i konvencionalnih rastvarača u ekstrakciji derivata hidroksicimetne kiseline iz herbe Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)",
volume = "72",
number = "4 suplement",
pages = "S481-S482",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4587"
}
Arsenijević, J., Drobac, M., Slavkovska, V., Dabetić, N., Marcic, C., Kovačević, N.,& Ražić, S.. (2022). Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S481-S482.
https://hdl.handle.net/21.15107/rcub_farfar_4587
Arsenijević J, Drobac M, Slavkovska V, Dabetić N, Marcic C, Kovačević N, Ražić S. Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae). in Arhiv za farmaciju. 2022;72(4 suplement):S481-S482.
https://hdl.handle.net/21.15107/rcub_farfar_4587 .
Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Dabetić, Nevena, Marcic, Christophe, Kovačević, Nada, Ražić, Slavica, "Comparison of efficiency of eutectic mixture and conventional solvents for the extraction of hydroxycinnamic acid derivatives from the herb of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae)" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S481-S482,
https://hdl.handle.net/21.15107/rcub_farfar_4587 .

In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions

Pavlović, Dragana; Stojanović, Nikola; Zlatković, Bojan; Radulović, Niko; Kovačević, Nada

(Savez farmaceutskih udruženja Srbije (SFUS), 2022)

TY  - CONF
AU  - Pavlović, Dragana
AU  - Stojanović, Nikola
AU  - Zlatković, Bojan
AU  - Radulović, Niko
AU  - Kovačević, Nada
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4579
AB  - According to our previous studies, Bruckenthalia spiculifolia Rchb. (Ericaceae) possess
strong antioxidant activity and hydrogel loaded with 2% ethanolic extract from its flowers
and leaves is a potential southing and wound healing agent (1). Ethanolic extracts of leaves,
flowers, and herba, alongside fractions obtained by systematic solvent extraction method
with solvents of the increasing polarity of liquid-liquid fractionation of areal parts of B.
spiculifolia, were prepared. To estimate in vitro anti-inflammatory activities of eight different
extracts and fractions, isolated rat peritoneal macrophages were employed. MTT test was
used for the cytotoxicity determination, while the anti-inflammatory potential
lipopolysaccharide-induced NO production model was utilized (2). Experimental procedures
with animals complied with the rules of the European Union Normative. Resident rat
peritoneal macrophages were harvested by peritoneal lavage and used for both procedures.
Phytochemical analysis was done by RP-HPLC coupled with DAD detection. Tested samples
statistically significantly decreased macrophage ability to metabolize MTT, mainly in a
concentration-dependent manner. All of them also significantly inhibited NO production
from macrophages stimulated with lipopolysaccharide, which can be related to either their
cytotoxic or anti-inflammatory activity. Quantified polar compounds (especially quercetin-3-
O-glucoside) could be associated with better antioxidant and anti-inflammatory effects, as
well as with a lower cytotoxic potential. Indeed, fractions obtained with ethyl acetate and
water, but also ethanol extract obtained after lipids removal with petroleum ether, showed
the most promising effects. These results point out that B. spiculifolia is worth further
investigation in order to detect new naturally occurring anti-inflammatory agents.
AB  - Prema našim prethodnim istraživanjima, Bruckenthalia spiculifolia Rchb. (Ericaceae)
poseduje jaku antioksidativnu aktivnost, a hidrogel sa 2% etanolnog ekstrakta cveta i lista
ove biljne vrste pospešuje zarastanje rana (1). Pripremljeni su etanolni ekstrakti listova,
cvetova i nadzemnog dela biljne vrste B. spiculifolia, kao i frakcije dobijene sistematskom
ekstrakcijom rastvaračima rastuć eg polariteta tečno-tečno frakcionisanjem nadzemnih
delova biljke. Za procenu lokalne antiinflamatorne aktivnosti ukupno osam različitih
ekstrakata i frakcija korišćeni su izolovani peritonealni makrofagi pacova. MTT test je služio
za određivanje citotoksičnosti, a model produkcije NO izazvane lipopolisaharidom za in vitro
kvantifikaciju antiinflamatornog potencijala (2). Eksperimentalne procedure sa životinjama
su usklađeni sa zahtevima Evropske unije. Peritonealni makrofagi pacova su sakupljeni
peritonealnim ispiranjem i korišćeni za obe procedure. Fitohemijska analiza je izvršna
pomoć u RP-HPLC sa DAD detekcijom. Testirani uzorci su statistički značajno smanjili
sposobnost makrofaga da metabolišu MTT i to uglavnom na dozno-zavisan način. Svi oni su
takođe značajno inhibirali proizvodnju NO iz makrofaga stimulisanih lipopolisaharidom, što
se može dovesti u vezu sa njihovom citotoksičnom ili antiinflamatornom aktivnošć u.
Kvantifikovana polarna jedinjenja (naročito kvercetin-3-O-glukozid) mogu biti povezana sa
boljim antioksidativnim i antiinflamatornim efektima, kao i sa nižim citotoksičnim
potencijalom. Zaista, frakcije dobijene sa etil acetatom i vodom, ali i etanolni ekstrakt dobijen
nakon uklanjanja lipida petroletrom, su pokazale najbolje efekte. Izloženi rezultati ukazuju
na to da vrstu B. spiculifolia treba dalje ispitati u cilju pronalaženja novih prirodnih
antiinflamatornih agensa.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions
T1  - In vitro citotoksična i antiinflamatorna aktivnost ekstrakata i frakcija Bruckenthalia spiculifolia
VL  - 72
IS  - 4 suplement
SP  - S453
EP  - S454
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4579
ER  - 
@conference{
author = "Pavlović, Dragana and Stojanović, Nikola and Zlatković, Bojan and Radulović, Niko and Kovačević, Nada",
year = "2022",
abstract = "According to our previous studies, Bruckenthalia spiculifolia Rchb. (Ericaceae) possess
strong antioxidant activity and hydrogel loaded with 2% ethanolic extract from its flowers
and leaves is a potential southing and wound healing agent (1). Ethanolic extracts of leaves,
flowers, and herba, alongside fractions obtained by systematic solvent extraction method
with solvents of the increasing polarity of liquid-liquid fractionation of areal parts of B.
spiculifolia, were prepared. To estimate in vitro anti-inflammatory activities of eight different
extracts and fractions, isolated rat peritoneal macrophages were employed. MTT test was
used for the cytotoxicity determination, while the anti-inflammatory potential
lipopolysaccharide-induced NO production model was utilized (2). Experimental procedures
with animals complied with the rules of the European Union Normative. Resident rat
peritoneal macrophages were harvested by peritoneal lavage and used for both procedures.
Phytochemical analysis was done by RP-HPLC coupled with DAD detection. Tested samples
statistically significantly decreased macrophage ability to metabolize MTT, mainly in a
concentration-dependent manner. All of them also significantly inhibited NO production
from macrophages stimulated with lipopolysaccharide, which can be related to either their
cytotoxic or anti-inflammatory activity. Quantified polar compounds (especially quercetin-3-
O-glucoside) could be associated with better antioxidant and anti-inflammatory effects, as
well as with a lower cytotoxic potential. Indeed, fractions obtained with ethyl acetate and
water, but also ethanol extract obtained after lipids removal with petroleum ether, showed
the most promising effects. These results point out that B. spiculifolia is worth further
investigation in order to detect new naturally occurring anti-inflammatory agents., Prema našim prethodnim istraživanjima, Bruckenthalia spiculifolia Rchb. (Ericaceae)
poseduje jaku antioksidativnu aktivnost, a hidrogel sa 2% etanolnog ekstrakta cveta i lista
ove biljne vrste pospešuje zarastanje rana (1). Pripremljeni su etanolni ekstrakti listova,
cvetova i nadzemnog dela biljne vrste B. spiculifolia, kao i frakcije dobijene sistematskom
ekstrakcijom rastvaračima rastuć eg polariteta tečno-tečno frakcionisanjem nadzemnih
delova biljke. Za procenu lokalne antiinflamatorne aktivnosti ukupno osam različitih
ekstrakata i frakcija korišćeni su izolovani peritonealni makrofagi pacova. MTT test je služio
za određivanje citotoksičnosti, a model produkcije NO izazvane lipopolisaharidom za in vitro
kvantifikaciju antiinflamatornog potencijala (2). Eksperimentalne procedure sa životinjama
su usklađeni sa zahtevima Evropske unije. Peritonealni makrofagi pacova su sakupljeni
peritonealnim ispiranjem i korišćeni za obe procedure. Fitohemijska analiza je izvršna
pomoć u RP-HPLC sa DAD detekcijom. Testirani uzorci su statistički značajno smanjili
sposobnost makrofaga da metabolišu MTT i to uglavnom na dozno-zavisan način. Svi oni su
takođe značajno inhibirali proizvodnju NO iz makrofaga stimulisanih lipopolisaharidom, što
se može dovesti u vezu sa njihovom citotoksičnom ili antiinflamatornom aktivnošć u.
Kvantifikovana polarna jedinjenja (naročito kvercetin-3-O-glukozid) mogu biti povezana sa
boljim antioksidativnim i antiinflamatornim efektima, kao i sa nižim citotoksičnim
potencijalom. Zaista, frakcije dobijene sa etil acetatom i vodom, ali i etanolni ekstrakt dobijen
nakon uklanjanja lipida petroletrom, su pokazale najbolje efekte. Izloženi rezultati ukazuju
na to da vrstu B. spiculifolia treba dalje ispitati u cilju pronalaženja novih prirodnih
antiinflamatornih agensa.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions, In vitro citotoksična i antiinflamatorna aktivnost ekstrakata i frakcija Bruckenthalia spiculifolia",
volume = "72",
number = "4 suplement",
pages = "S453-S454",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4579"
}
Pavlović, D., Stojanović, N., Zlatković, B., Radulović, N.,& Kovačević, N.. (2022). In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S453-S454.
https://hdl.handle.net/21.15107/rcub_farfar_4579
Pavlović D, Stojanović N, Zlatković B, Radulović N, Kovačević N. In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions. in Arhiv za farmaciju. 2022;72(4 suplement):S453-S454.
https://hdl.handle.net/21.15107/rcub_farfar_4579 .
Pavlović, Dragana, Stojanović, Nikola, Zlatković, Bojan, Radulović, Niko, Kovačević, Nada, "In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S453-S454,
https://hdl.handle.net/21.15107/rcub_farfar_4579 .

The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)

Slavkovska, Violeta; Zlatković, Bojan; Marčetić, Mirjana; Kovačević, Nada; Drobac, Milica

(Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, 2022)

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4319
AB  - This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far.
AB  - Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)
T1  - Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)
VL  - 46
IS  - 2
SP  - 269
EP  - 284
DO  - 10.2298/BOTSERB2202269S
ER  - 
@article{
author = "Slavkovska, Violeta and Zlatković, Bojan and Marčetić, Mirjana and Kovačević, Nada and Drobac, Milica",
year = "2022",
abstract = "This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far., Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae), Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)",
volume = "46",
number = "2",
pages = "269-284",
doi = "10.2298/BOTSERB2202269S"
}
Slavkovska, V., Zlatković, B., Marčetić, M., Kovačević, N.,& Drobac, M.. (2022). The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 46(2), 269-284.
https://doi.org/10.2298/BOTSERB2202269S
Slavkovska V, Zlatković B, Marčetić M, Kovačević N, Drobac M. The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica. 2022;46(2):269-284.
doi:10.2298/BOTSERB2202269S .
Slavkovska, Violeta, Zlatković, Bojan, Marčetić, Mirjana, Kovačević, Nada, Drobac, Milica, "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)" in Botanica Serbica, 46, no. 2 (2022):269-284,
https://doi.org/10.2298/BOTSERB2202269S . .

Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil

Suručić, Relja; Kundaković-Vasović, Tatjana; Marčetić, Mirjana; Drakul, Dragana; Milenković, Marina; Kovačević, Nada

(Belgrade : Military Medical Academy, INI, 2021)

TY  - JOUR
AU  - Suručić, Relja
AU  - Kundaković-Vasović, Tatjana
AU  - Marčetić, Mirjana
AU  - Drakul, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4002
AB  - Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil.
AB  - Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil
T1  - Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)
VL  - 78
IS  - 10
SP  - 1065
EP  - 1071
DO  - 10.2298/VSP190320028S
ER  - 
@article{
author = "Suručić, Relja and Kundaković-Vasović, Tatjana and Marčetić, Mirjana and Drakul, Dragana and Milenković, Marina and Kovačević, Nada",
year = "2021",
abstract = "Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil., Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil, Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)",
volume = "78",
number = "10",
pages = "1065-1071",
doi = "10.2298/VSP190320028S"
}
Suručić, R., Kundaković-Vasović, T., Marčetić, M., Drakul, D., Milenković, M.,& Kovačević, N.. (2021). Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 78(10), 1065-1071.
https://doi.org/10.2298/VSP190320028S
Suručić R, Kundaković-Vasović T, Marčetić M, Drakul D, Milenković M, Kovačević N. Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled. 2021;78(10):1065-1071.
doi:10.2298/VSP190320028S .
Suručić, Relja, Kundaković-Vasović, Tatjana, Marčetić, Mirjana, Drakul, Dragana, Milenković, Marina, Kovačević, Nada, "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil" in Vojnosanitetski pregled, 78, no. 10 (2021):1065-1071,
https://doi.org/10.2298/VSP190320028S . .
2
2

Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents

Ilić, Milan; Samardžić, Stevan; Kotur-Stevuljević, Jelena; Ušjak, Dušan; Milenković, Marina; Kovačević, Nada; Drobac, Milica

(Verduci Editore s.r.l, 2021)

TY  - JOUR
AU  - Ilić, Milan
AU  - Samardžić, Stevan
AU  - Kotur-Stevuljević, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3995
AB  - Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents
VL  - 25
IS  - 20
SP  - 6283
EP  - 6294
DO  - 10.26355/eurrev_202110_26998
ER  - 
@article{
author = "Ilić, Milan and Samardžić, Stevan and Kotur-Stevuljević, Jelena and Ušjak, Dušan and Milenković, Marina and Kovačević, Nada and Drobac, Milica",
year = "2021",
abstract = "Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents",
volume = "25",
number = "20",
pages = "6283-6294",
doi = "10.26355/eurrev_202110_26998"
}
Ilić, M., Samardžić, S., Kotur-Stevuljević, J., Ušjak, D., Milenković, M., Kovačević, N.,& Drobac, M.. (2021). Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 25(20), 6283-6294.
https://doi.org/10.26355/eurrev_202110_26998
Ilić M, Samardžić S, Kotur-Stevuljević J, Ušjak D, Milenković M, Kovačević N, Drobac M. Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences. 2021;25(20):6283-6294.
doi:10.26355/eurrev_202110_26998 .
Ilić, Milan, Samardžić, Stevan, Kotur-Stevuljević, Jelena, Ušjak, Dušan, Milenković, Marina, Kovačević, Nada, Drobac, Milica, "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents" in European Review for Medical and Pharmacological Sciences, 25, no. 20 (2021):6283-6294,
https://doi.org/10.26355/eurrev_202110_26998 . .
2
2

Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents

Drobac, Milica; Omar, Eihab; Kovačević, Nada

(2021)

TY  - CONF
AU  - Drobac, Milica
AU  - Omar, Eihab
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5245
AB  - The plants of genus Cymbopogon (family Poaceae) are well known for their use in traditional medicine and for the high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the inflorescences of Cymbopogon nervatus (Hochst.) Chiov. and C. schoenanthus (L.) Spreng. from Sudan were studied. GC-FID/MS analysis showed that C. nervatus essential oils is characterized by the high content of p-menthadienols (64.0-76.2%), whereas oils of C. schoenanthus are rich in monoterpene piperitone (47.7-58.7%). The oils were tested for spasmolytic activity on isolated rat ileum, using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The essential oils of both species exhibited strong, significant and dose-dependent spasmolytic activity. In addition, essential oils were investigated for antimicrobial activity by the microdilution method. Both oils were active against the strains of Staphylococcus aureus (MICs 0.85-1.3 mg/ml), Staphylococcus epidermidis (MICs 0.78-1.3 mg/ml) and Candida albicans (MICs 0.63-1.3 mg/ml), whereas Gram (-) bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) were resistant. The observed spasmolytic effect of the inflorescence essential oils of C. nervatus and C. schoenanthus emphasizes its beneficial properties and is in agreement with their traditional use, especially for gastrointestinal ailments.
C3  - International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual
T1  - Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5245
ER  - 
@conference{
author = "Drobac, Milica and Omar, Eihab and Kovačević, Nada",
year = "2021",
abstract = "The plants of genus Cymbopogon (family Poaceae) are well known for their use in traditional medicine and for the high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the inflorescences of Cymbopogon nervatus (Hochst.) Chiov. and C. schoenanthus (L.) Spreng. from Sudan were studied. GC-FID/MS analysis showed that C. nervatus essential oils is characterized by the high content of p-menthadienols (64.0-76.2%), whereas oils of C. schoenanthus are rich in monoterpene piperitone (47.7-58.7%). The oils were tested for spasmolytic activity on isolated rat ileum, using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The essential oils of both species exhibited strong, significant and dose-dependent spasmolytic activity. In addition, essential oils were investigated for antimicrobial activity by the microdilution method. Both oils were active against the strains of Staphylococcus aureus (MICs 0.85-1.3 mg/ml), Staphylococcus epidermidis (MICs 0.78-1.3 mg/ml) and Candida albicans (MICs 0.63-1.3 mg/ml), whereas Gram (-) bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) were resistant. The observed spasmolytic effect of the inflorescence essential oils of C. nervatus and C. schoenanthus emphasizes its beneficial properties and is in agreement with their traditional use, especially for gastrointestinal ailments.",
journal = "International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual",
title = "Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5245"
}
Drobac, M., Omar, E.,& Kovačević, N.. (2021). Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents. in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual.
https://hdl.handle.net/21.15107/rcub_farfar_5245
Drobac M, Omar E, Kovačević N. Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents. in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5245 .
Drobac, Milica, Omar, Eihab, Kovačević, Nada, "Essential oils of Cymbopogon Spreng. species - potential spasmolytic agents" in International Conference on Traditional Medicine and Phytochemistry - TMED 2021/Virtual (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5245 .

Green approach to extraction of plant volatiles from herbal teas using ionic liquids

Arsenijević, Jelena; Drobac, Milica; Trtić-Petrović, Tatjana; Kovačević, Nada; Ražić, Slavica

(European Chemical Society, 2021)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Trtić-Petrović, Tatjana
AU  - Kovačević, Nada
AU  - Ražić, Slavica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5158
PB  - European Chemical Society
C3  - 1st European Sample Preparation e-Conference, March 11-12, 2021, Book of Abstracts
T1  - Green approach to extraction of plant volatiles from herbal teas using ionic liquids
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5158
ER  - 
@conference{
author = "Arsenijević, Jelena and Drobac, Milica and Trtić-Petrović, Tatjana and Kovačević, Nada and Ražić, Slavica",
year = "2021",
publisher = "European Chemical Society",
journal = "1st European Sample Preparation e-Conference, March 11-12, 2021, Book of Abstracts",
title = "Green approach to extraction of plant volatiles from herbal teas using ionic liquids",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5158"
}
Arsenijević, J., Drobac, M., Trtić-Petrović, T., Kovačević, N.,& Ražić, S.. (2021). Green approach to extraction of plant volatiles from herbal teas using ionic liquids. in 1st European Sample Preparation e-Conference, March 11-12, 2021, Book of Abstracts
European Chemical Society..
https://hdl.handle.net/21.15107/rcub_farfar_5158
Arsenijević J, Drobac M, Trtić-Petrović T, Kovačević N, Ražić S. Green approach to extraction of plant volatiles from herbal teas using ionic liquids. in 1st European Sample Preparation e-Conference, March 11-12, 2021, Book of Abstracts. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5158 .
Arsenijević, Jelena, Drobac, Milica, Trtić-Petrović, Tatjana, Kovačević, Nada, Ražić, Slavica, "Green approach to extraction of plant volatiles from herbal teas using ionic liquids" in 1st European Sample Preparation e-Conference, March 11-12, 2021, Book of Abstracts (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5158 .

Pharmacy and nature ‐ plenty of diversity

Kovačević, Nada

(Savez farmaceutskih udruženja Srbije (SFUS), 2021)

TY  - CONF
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5106
AB  - In the most general sense, nature represents the unity of soil, water and air and all living beings. Man and all his activities affect life on our planet in different ways, often through gross violations of natural laws and fine, subtle connections and mutual influences of the environment and different species and forms of the living world. Numerous initiatives over the last decades indicate that every human activity and "world of people" should be observed, estimated and evaluated from the aspect of environmental impact. The development of the economy and society as a whole must be based on technologies that are in harmony with nature and that use natural resources in an organized and controlled manner that does not lead to devastation, violation of natural laws, and environmental disasters.

In the areas of health, preservation and improvement of the health of individuals and society, nature is important not only as a source of pharmaceutically active and auxiliary substances, but also as a source of ideas, problems and solutions that are hidden somewhere, and usually simple, but should be discovered and understood. From the idea of ​​a pharmaceutical product, through the development of materials and technologies for their production, analysis and control, application in different patient populations, to the proper disposal and destruction of used and unused materials and products, everything is connected and has an impact on land, water and air, as well as to all living beings on this planet. In the scientific and research sense, but also through the professional aspect of pharmacy, pharmacognosy is most often associated with nature, as the main source of natural medicinal products.

In recent decades, pharmacognosy, as a concept and as a scientific discipline within the pharmaceutical sciences, has changed its focus. From the applied, descriptive botanical science, from the sixties of the last century, it moved to the chemical, biochemical and economic aspects of natural products (drugs) and their ingredients. And then in the 21st century, it becomes "a science that focuses on the study of the relationship between the structure and activity of ingredients, the therapeutic and use potential that natural products (drugs) have because of the ingredients they contain." Scientific pharmacognosy, today, should also include concrete assistance to herbal medicine manufacturers to provide safe and effective products; through research into bioavailability, bioequivalence, through standardization and quality control, but also through proper design of pharmaceutical forms and clinical studies to examine the efficacy of these medicinal products. Also, due to the growing number of different kind of products on the market, whose application is based on the presence of secondary metabolites of plants, it is necessary for pharmacists to be able to essentially understand those products, in proper, quality and safe way to produce, control and recommend them to users (1,2,3).

Fundamental research within pharmacognosy still aims to use the biological diversity of plants, algae, mosses, fungi, animals and microorganisms (on land and in the aquatic environment) to discover new compounds with some pharmacological activities. Diversity in nature is not only seen through the number of living organisms. The diversity of chemically pharmacologically active structures, isolated from natural organisms, is very significant. According to certain authors, these compounds have more numerous and complex steric structures in relation to those obtained by organic synthesis. There are about 30,000 of them in the databases; about 13% were isolated from animals, 33% from bacteria, 26% from fungi, and 27% from plants (1).

We are still looking for medicinal substances that will prevent the growth of malignant cells, which will prevent or cure inflammatory processes, prevent and cure infection caused by pathogenic microorganisms, and affect neurodegenerative changes. Isolated compounds are used as such, their structure is partially changed or they serve as a model of a substance for the synthesis of completely new medicinal compounds. Here are described several examples of drugs whose active components originate from plants or have partially altered structures (acarbosa, artemeter, docetaxel, paclitaxel, galantamine, camptothecin-irinothecan-topothecan...) (1). Prostratin (Homolanthus nutans, Euphorbiaceae), resveratrol, huperzine A, as well as artemisinin or betulinic acid are still the subject of clinical trials.

Also, herbal extracts are still being studied as active ingredients of herbal medicines, which achieve effects through the synergism of their ingredients. Researchers in the field of natural products are also engaged in current research on the use of waste materials to create new useful products and more complete use of biomass. In the presentation itself, different approaches to the research of natural medicinal products will be explained and documented in more detail.

It should also be emphasized that the largest amount of plant raw material for the isolation of compounds or the production of extracts is now produced by field production; production by fermentation of microorganisms is also used, and very rarely in bioreactors using cell cultures (taxol and ginseng biomass, for example). Equipment and technology are available, but problems arise regarding the price of the products obtained in this way.
AB  - Naopštije posmatrano, priroda predstavlja jedinstvo zemljišta, vode i vazduha i svih
živih bića. Čovek i sve njegove aktivnosti utiču na život na našoj planeti na različite načine,
često kroz grubo narušavanje prirodnih zakonitosti i fine, suptilne povezanosti i međusobnih
uticaja sredine i različitih vrsta i oblika živog sveta. Brojne inicijative u toku poslednjih decenija
ukazuju da svaka aktivnost čoveka i “sveta ljudi” treba da se posmatra, procenjuje i vrednuje i
sa aspekta uticaja na životnu sredinu. Razvoj ekonomije i društva u celini mora biti zasnovan
na tehnologijama koje su u saglasju sa prirodom i koje prirodne resurse koriste na organizovan
i kontrolisan način koji ne dovodi do devastiranja, narušavanja prirodnih zakonitosti i
ekoloških katastrofa.
Za oblast zdravstva, očuvanje i unapređenje zdravlja pojedinca i društva, priroda je
važna ne samo kao izvor farmaceutski aktivnih i pomoćnih supstanci, već i kao izvor ideja,
problema i rešenja koja su negde skrivena, i najčešće jednostavna, ali ih treba otkriti i razumeti.
Od ideje za farmaceutski proizvod, preko razvoja materijala i tehnologija za njihovu
proizvodnju, analizu i kontrolu, primene kod različitih populacija pacijenata, do pravilnog
odlaganja i uništavanja iskorišćenog i neiskorišćenog materijala i proizvoda, sve je povezano i
ima uticaj na zemlju, vodu i vazduh, kao i na sva živa bića na ovoj planeti. U naučnom i
istraživačkom smislu, ali i kroz profesionalni aspekt farmacije, farmakognozija se najčešće
povezuje sa prirodom, kao glavnim izvorom prirodnih lekovitih proizvoda.
Poslednjih decenija, farmakognozija, kao pojam i kao naučna disciplina u okviru
farmaceutskih nauka, menja svoje težište. Od primenjene, deskriptive botaničke nauke, od
šezdesetih godina prošlog veka, pomerala se ka hemijskim, biohemijskim i ekonomskim
aspektima prirodnih proizvoda (droga) i njihovih sastojaka. A onda u 21. veku postaje
„nauka koja u centru interesovanja ima proučavanje odnosa strukture i aktivnosti sastojaka,
terapijskog i upotrebnog potencijala koji imaju prirodni proizvodi (droge) zbog sastojaka
koje sadrže“. Naučna farmakognozija, danas, treba da obuhvati i konkretnu pomoć
proizvođačima biljnih lekova za obezbeđivanje sigurnih i efikasnih proizvoda; kroz
istraživanje bioraspoloživosti, bioekvivalencije, kroz standardizaciju i kontrolu kvaliteta, ali i
kroz pravilno dizajniranje farmaceutskih oblika i kliničkih studije za ispitivanje efikasnosti
ovih lekovitih proizvoda. Takođe, zbog sve većeg broja različitih proizvoda na tržištu, čija je
primena zasnovana na prisustvu sekundarnih metabolita biljaka, neophodno je da
farmaceuti budu osposobljeni da ove proizvode suštinski razumeju, na pravilan, kvalitetan i
bezbedan način proizvedu, kontrolišu, preporuče korisnicima (1,2,3).
Fundamentalna istraživanja u okviru farmakognozije i dalje imaju cilj da biološku
razvnovrsnost biljaka, algi, mahovina, gljiva, životinja i mikroorganizama (na kopnu i u
vodenoj sredini) iskoriste da otkriju nova jedinjenja, nosioce određenih farmakoloških
aktivnosti. Diverzitet u prirodi se ne sagledava samo kroz broj živih organizama. Veoma je
značajan diverzitet hemijskih farmakološki aktivnih struktura, izolovanih iz prirodnih
organizama. Po određenim autorima, ove jedinjenja su brojnija i komleksnije sterne
strukture u odnosu na ona dobijena organskom sintezoma. U bazama podataka ima ih oko
30000; oko 13% je izolovano iz životinja, 33% iz bakterija, 26 % iz gljiva i 27 % iz biljaka (1).
I dalje se traga za lekovitim supstancama koje će sprečiti rast malignih ćelija, koje će
sprečiti ili izlečiti procese inflamacije, onemogućiti i izlečiti infekciju patogenim
S | 8
mikroorganizmima i uticati na neurodegenerativne promene. Izolovana jedinjenja se koriste
kao takva, delimično im se menja struktura ili služe kao model supstance za sintezu sasvim
novih lekovitih jedinjenja. Ovde je dato nekoliko primera lekova čije aktivne komponente
vode poreklo od biljaka ili su delimično izmenjene strukture (akarbosa, artemeter,
docetaksel, paklitaksel, galantamin, kamptotecin-irinotekan, topotekan...) (1). Prostratin
(Homolanthus nutans, Euphorbiaceae), resveratrol, huperzin A, kao i artemisinin ili
betulinska kiselina i dalje su predmet kliničkih ispitivanja.
Takođe, i dalje se proučavaju biljni ekstrakti, kao aktivni sastojci biljnih lekova, koji
efekte ostvaruju kroz sinergizam svojih sastojaka. Istraživači u oblasti prirodnih proizvoda,
bave se i aktuelnim istraživanja iskorišćenja odpadnih materijala za stvaranje novih korisnih
proizvoda i potpunije korišćenje biomase. U samom izlaganju, različiti pristupi istraživanja
prirodnih lekovitih proizvoda će biti detaljnije objašnjeni i dokumentovani.
Treba, takođe, naglasiti da se najveća količina biljne sirovine za izolaciju jedinjenja ili
izradu ekstrakata, danas proizvodi ratarskom proizvodnjom; koristi se i proizvodnja
fermentacijom mikroorganizama, a sasvim retko u bioreaktorima korišćenjem ćelijskih
kultura (taksol i biomasa žen-šena, npr.). Oprema i tehnologija su dostupne, ali problemi se
javljaju kod cene ovako dobijenih proizvoda.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Pharmacy and nature ‐ plenty of diversity
T1  - Farmacija i priroda – bogatstvo diverziteta
VL  - 71
IS  - 5 suplement
SP  - S5
EP  - S8
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5106
ER  - 
@conference{
author = "Kovačević, Nada",
year = "2021",
abstract = "In the most general sense, nature represents the unity of soil, water and air and all living beings. Man and all his activities affect life on our planet in different ways, often through gross violations of natural laws and fine, subtle connections and mutual influences of the environment and different species and forms of the living world. Numerous initiatives over the last decades indicate that every human activity and "world of people" should be observed, estimated and evaluated from the aspect of environmental impact. The development of the economy and society as a whole must be based on technologies that are in harmony with nature and that use natural resources in an organized and controlled manner that does not lead to devastation, violation of natural laws, and environmental disasters.

In the areas of health, preservation and improvement of the health of individuals and society, nature is important not only as a source of pharmaceutically active and auxiliary substances, but also as a source of ideas, problems and solutions that are hidden somewhere, and usually simple, but should be discovered and understood. From the idea of ​​a pharmaceutical product, through the development of materials and technologies for their production, analysis and control, application in different patient populations, to the proper disposal and destruction of used and unused materials and products, everything is connected and has an impact on land, water and air, as well as to all living beings on this planet. In the scientific and research sense, but also through the professional aspect of pharmacy, pharmacognosy is most often associated with nature, as the main source of natural medicinal products.

In recent decades, pharmacognosy, as a concept and as a scientific discipline within the pharmaceutical sciences, has changed its focus. From the applied, descriptive botanical science, from the sixties of the last century, it moved to the chemical, biochemical and economic aspects of natural products (drugs) and their ingredients. And then in the 21st century, it becomes "a science that focuses on the study of the relationship between the structure and activity of ingredients, the therapeutic and use potential that natural products (drugs) have because of the ingredients they contain." Scientific pharmacognosy, today, should also include concrete assistance to herbal medicine manufacturers to provide safe and effective products; through research into bioavailability, bioequivalence, through standardization and quality control, but also through proper design of pharmaceutical forms and clinical studies to examine the efficacy of these medicinal products. Also, due to the growing number of different kind of products on the market, whose application is based on the presence of secondary metabolites of plants, it is necessary for pharmacists to be able to essentially understand those products, in proper, quality and safe way to produce, control and recommend them to users (1,2,3).

Fundamental research within pharmacognosy still aims to use the biological diversity of plants, algae, mosses, fungi, animals and microorganisms (on land and in the aquatic environment) to discover new compounds with some pharmacological activities. Diversity in nature is not only seen through the number of living organisms. The diversity of chemically pharmacologically active structures, isolated from natural organisms, is very significant. According to certain authors, these compounds have more numerous and complex steric structures in relation to those obtained by organic synthesis. There are about 30,000 of them in the databases; about 13% were isolated from animals, 33% from bacteria, 26% from fungi, and 27% from plants (1).

We are still looking for medicinal substances that will prevent the growth of malignant cells, which will prevent or cure inflammatory processes, prevent and cure infection caused by pathogenic microorganisms, and affect neurodegenerative changes. Isolated compounds are used as such, their structure is partially changed or they serve as a model of a substance for the synthesis of completely new medicinal compounds. Here are described several examples of drugs whose active components originate from plants or have partially altered structures (acarbosa, artemeter, docetaxel, paclitaxel, galantamine, camptothecin-irinothecan-topothecan...) (1). Prostratin (Homolanthus nutans, Euphorbiaceae), resveratrol, huperzine A, as well as artemisinin or betulinic acid are still the subject of clinical trials.

Also, herbal extracts are still being studied as active ingredients of herbal medicines, which achieve effects through the synergism of their ingredients. Researchers in the field of natural products are also engaged in current research on the use of waste materials to create new useful products and more complete use of biomass. In the presentation itself, different approaches to the research of natural medicinal products will be explained and documented in more detail.

It should also be emphasized that the largest amount of plant raw material for the isolation of compounds or the production of extracts is now produced by field production; production by fermentation of microorganisms is also used, and very rarely in bioreactors using cell cultures (taxol and ginseng biomass, for example). Equipment and technology are available, but problems arise regarding the price of the products obtained in this way., Naopštije posmatrano, priroda predstavlja jedinstvo zemljišta, vode i vazduha i svih
živih bića. Čovek i sve njegove aktivnosti utiču na život na našoj planeti na različite načine,
često kroz grubo narušavanje prirodnih zakonitosti i fine, suptilne povezanosti i međusobnih
uticaja sredine i različitih vrsta i oblika živog sveta. Brojne inicijative u toku poslednjih decenija
ukazuju da svaka aktivnost čoveka i “sveta ljudi” treba da se posmatra, procenjuje i vrednuje i
sa aspekta uticaja na životnu sredinu. Razvoj ekonomije i društva u celini mora biti zasnovan
na tehnologijama koje su u saglasju sa prirodom i koje prirodne resurse koriste na organizovan
i kontrolisan način koji ne dovodi do devastiranja, narušavanja prirodnih zakonitosti i
ekoloških katastrofa.
Za oblast zdravstva, očuvanje i unapređenje zdravlja pojedinca i društva, priroda je
važna ne samo kao izvor farmaceutski aktivnih i pomoćnih supstanci, već i kao izvor ideja,
problema i rešenja koja su negde skrivena, i najčešće jednostavna, ali ih treba otkriti i razumeti.
Od ideje za farmaceutski proizvod, preko razvoja materijala i tehnologija za njihovu
proizvodnju, analizu i kontrolu, primene kod različitih populacija pacijenata, do pravilnog
odlaganja i uništavanja iskorišćenog i neiskorišćenog materijala i proizvoda, sve je povezano i
ima uticaj na zemlju, vodu i vazduh, kao i na sva živa bića na ovoj planeti. U naučnom i
istraživačkom smislu, ali i kroz profesionalni aspekt farmacije, farmakognozija se najčešće
povezuje sa prirodom, kao glavnim izvorom prirodnih lekovitih proizvoda.
Poslednjih decenija, farmakognozija, kao pojam i kao naučna disciplina u okviru
farmaceutskih nauka, menja svoje težište. Od primenjene, deskriptive botaničke nauke, od
šezdesetih godina prošlog veka, pomerala se ka hemijskim, biohemijskim i ekonomskim
aspektima prirodnih proizvoda (droga) i njihovih sastojaka. A onda u 21. veku postaje
„nauka koja u centru interesovanja ima proučavanje odnosa strukture i aktivnosti sastojaka,
terapijskog i upotrebnog potencijala koji imaju prirodni proizvodi (droge) zbog sastojaka
koje sadrže“. Naučna farmakognozija, danas, treba da obuhvati i konkretnu pomoć
proizvođačima biljnih lekova za obezbeđivanje sigurnih i efikasnih proizvoda; kroz
istraživanje bioraspoloživosti, bioekvivalencije, kroz standardizaciju i kontrolu kvaliteta, ali i
kroz pravilno dizajniranje farmaceutskih oblika i kliničkih studije za ispitivanje efikasnosti
ovih lekovitih proizvoda. Takođe, zbog sve većeg broja različitih proizvoda na tržištu, čija je
primena zasnovana na prisustvu sekundarnih metabolita biljaka, neophodno je da
farmaceuti budu osposobljeni da ove proizvode suštinski razumeju, na pravilan, kvalitetan i
bezbedan način proizvedu, kontrolišu, preporuče korisnicima (1,2,3).
Fundamentalna istraživanja u okviru farmakognozije i dalje imaju cilj da biološku
razvnovrsnost biljaka, algi, mahovina, gljiva, životinja i mikroorganizama (na kopnu i u
vodenoj sredini) iskoriste da otkriju nova jedinjenja, nosioce određenih farmakoloških
aktivnosti. Diverzitet u prirodi se ne sagledava samo kroz broj živih organizama. Veoma je
značajan diverzitet hemijskih farmakološki aktivnih struktura, izolovanih iz prirodnih
organizama. Po određenim autorima, ove jedinjenja su brojnija i komleksnije sterne
strukture u odnosu na ona dobijena organskom sintezoma. U bazama podataka ima ih oko
30000; oko 13% je izolovano iz životinja, 33% iz bakterija, 26 % iz gljiva i 27 % iz biljaka (1).
I dalje se traga za lekovitim supstancama koje će sprečiti rast malignih ćelija, koje će
sprečiti ili izlečiti procese inflamacije, onemogućiti i izlečiti infekciju patogenim
S | 8
mikroorganizmima i uticati na neurodegenerativne promene. Izolovana jedinjenja se koriste
kao takva, delimično im se menja struktura ili služe kao model supstance za sintezu sasvim
novih lekovitih jedinjenja. Ovde je dato nekoliko primera lekova čije aktivne komponente
vode poreklo od biljaka ili su delimično izmenjene strukture (akarbosa, artemeter,
docetaksel, paklitaksel, galantamin, kamptotecin-irinotekan, topotekan...) (1). Prostratin
(Homolanthus nutans, Euphorbiaceae), resveratrol, huperzin A, kao i artemisinin ili
betulinska kiselina i dalje su predmet kliničkih ispitivanja.
Takođe, i dalje se proučavaju biljni ekstrakti, kao aktivni sastojci biljnih lekova, koji
efekte ostvaruju kroz sinergizam svojih sastojaka. Istraživači u oblasti prirodnih proizvoda,
bave se i aktuelnim istraživanja iskorišćenja odpadnih materijala za stvaranje novih korisnih
proizvoda i potpunije korišćenje biomase. U samom izlaganju, različiti pristupi istraživanja
prirodnih lekovitih proizvoda će biti detaljnije objašnjeni i dokumentovani.
Treba, takođe, naglasiti da se najveća količina biljne sirovine za izolaciju jedinjenja ili
izradu ekstrakata, danas proizvodi ratarskom proizvodnjom; koristi se i proizvodnja
fermentacijom mikroorganizama, a sasvim retko u bioreaktorima korišćenjem ćelijskih
kultura (taksol i biomasa žen-šena, npr.). Oprema i tehnologija su dostupne, ali problemi se
javljaju kod cene ovako dobijenih proizvoda.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Pharmacy and nature ‐ plenty of diversity, Farmacija i priroda – bogatstvo diverziteta",
volume = "71",
number = "5 suplement",
pages = "S5-S8",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5106"
}
Kovačević, N.. (2021). Pharmacy and nature ‐ plenty of diversity. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S5-S8.
https://hdl.handle.net/21.15107/rcub_farfar_5106
Kovačević N. Pharmacy and nature ‐ plenty of diversity. in Arhiv za farmaciju. 2021;71(5 suplement):S5-S8.
https://hdl.handle.net/21.15107/rcub_farfar_5106 .
Kovačević, Nada, "Pharmacy and nature ‐ plenty of diversity" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S5-S8,
https://hdl.handle.net/21.15107/rcub_farfar_5106 .

Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii

Arsenijević, Jelena; Kovačević, Nada; Drobac, Milica; Ražić, Slavica; Emhemmed, Fathi; Muller, Christian; Marcic, Christophe; Marchioni, Eric

(University Prince of Songkla, Thailand, 2021)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Kovačević, Nada
AU  - Drobac, Milica
AU  - Ražić, Slavica
AU  - Emhemmed, Fathi
AU  - Muller, Christian
AU  - Marcic, Christophe
AU  - Marchioni, Eric
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5103
AB  - INTRODUCTION:
Aerial parts of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae), in Serbia known as Rtanj’s tea, are traditionally used to treat various respiratory, urinary and other health disorders. Extracts of this herb exhibit a significant bioactivity as well37. Using
deep eutectic solvents (DESs) for extraction of certain phenolic compounds is in line with the principles of green chemistry38.
However, the toxicity of DESs must be considered39.
OBJECTIVES:
The main objectives of this work are to assess the polyphenol-extracting ability of proline (Pro) and sugar/sugar alcohol
based natural DESs from commercially available Rtanj’s tea, as well as to evaluate cytotoxicity of these solvents against AsPC-
1 cells.
METHOD / DESIGN:
Accurately weighed Pro and sugar/sugar alcohol, were dissolved in water, frozen, and freeze-dried. The obtained seven DESs
(i.e. Pro with: glucose 1:1 and 5:3, fructose 1:1 and 5:3, sorbitol 1:2, and sucrose 2:1 and 3:2) were mixed with water (30%,
m/m). Polyphenol extraction was examined using commercial sample of Rtanj’s tea (manufacturer Bojan Radosavljević, Boljevac).
Powdered herb (particle diameter 100-200 μm) was extracted by sonication during 30 min at room temperature with
the obtained aqueous DESs (herb-to-solvent ratio 1:20), as well as with water, absolute ethanol, or 50% (v/v) ethanol under
the same conditions. The qualitative analysis of extracts was conducted by both HPLC and LC-MS. The content of the identified
marker compounds in the extracts, i.e. rosmarinic acid (RA) and clinopodic acid O (CAO), was determined by external
calibration using RA as the standard compound. Cytotoxicity of the aqueous DESs against human pancreatic adenocarcinoma
cells AsPC-1 was tested at four concentration levels (5-25%), after 48 h of incubation and propidium iodide staining.
The analysis on Guava® easyCyte 12HT Benchtop flow microcapillary cytometer, was performed afterwards, using InCyte®
software package.
RESULTS:
Upon freeze-drying, mixtures had glassy appearance and transformed into liquids after mild heating. All obtained DESs were
highly viscous, and therefore mixed with water. Qualitative LC-MS analysis of 50% ethanol extract revealed the presence of
phenolic acids, flavonoids, and jasmonic acid derivatives. Among phenolic acids, the dominant compounds were caffeic
acid oligomers RA and CAO. Among conventional solvents, 50% ethanol was better extracting agent than absolute ethanol
or water for both RA (88.2 μg/mL) and CAO (116.8 μg/mL). Water extract was also abundant with CAO (106.7 μg/mL), but
contained moderate amount of RA (21.7 μg/mL). It is noteworthy to mention that the extraction with ethanol resulted in
very low yield of both phenolics, with CAO concentration, being even below detection limit in the absolute ethanol extract.
Concentration of RA in the tested DES extracts was higher than corresponding one in the water extract, but lower than in
the 50% ethanol extract, and varied in the range from 61.6 μg/mL (in Pro-fructose 1:1 extract) to 85.6 μg/mL (Pro-glucose 1:1
extract). The extraction of CAO, with six out of seven aqueous DESs, was more efficient than with 50% ethanol, resulting in
CAO concentration range from 119.6 μg/mL, in Pro-sucrose 3:2 extract, to 172.4 μg/mL, in Pro-glucose 1:1 extract. Pro-fructose
1:1 extract had the lowest content of CAO (86.7 μg/mL) among the tested DESs. At the lowest tested concentration (5%),
aqueous DESs did not significantly affect survival of AsPC-1 cells in comparison to the untreated cells (83.1-90.0% and 86.6%
of cells remained viable, respectively). Both proline-glucose DESs demonstrated the lowest toxicity. However, at the highest
concentration (25%) all aqueous DESs caused death of more than 70% of AsPC-1 cells. CONCLUSIONS:
The obtained results indicate that proline and sugar/sugar alcohol based deep eutectic solvents are good extracting agents
for phenolic compounds, especially for higher caffeic acid oligomers such as clinopodic acid O. Additionally, low cytotoxicity
of tested DESs is a good starting predictor of their safety and potential usage.
PB  - University Prince of Songkla, Thailand
PB  - University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
T1  - Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii
SP  - 31
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5103
ER  - 
@conference{
author = "Arsenijević, Jelena and Kovačević, Nada and Drobac, Milica and Ražić, Slavica and Emhemmed, Fathi and Muller, Christian and Marcic, Christophe and Marchioni, Eric",
year = "2021",
abstract = "INTRODUCTION:
Aerial parts of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae), in Serbia known as Rtanj’s tea, are traditionally used to treat various respiratory, urinary and other health disorders. Extracts of this herb exhibit a significant bioactivity as well37. Using
deep eutectic solvents (DESs) for extraction of certain phenolic compounds is in line with the principles of green chemistry38.
However, the toxicity of DESs must be considered39.
OBJECTIVES:
The main objectives of this work are to assess the polyphenol-extracting ability of proline (Pro) and sugar/sugar alcohol
based natural DESs from commercially available Rtanj’s tea, as well as to evaluate cytotoxicity of these solvents against AsPC-
1 cells.
METHOD / DESIGN:
Accurately weighed Pro and sugar/sugar alcohol, were dissolved in water, frozen, and freeze-dried. The obtained seven DESs
(i.e. Pro with: glucose 1:1 and 5:3, fructose 1:1 and 5:3, sorbitol 1:2, and sucrose 2:1 and 3:2) were mixed with water (30%,
m/m). Polyphenol extraction was examined using commercial sample of Rtanj’s tea (manufacturer Bojan Radosavljević, Boljevac).
Powdered herb (particle diameter 100-200 μm) was extracted by sonication during 30 min at room temperature with
the obtained aqueous DESs (herb-to-solvent ratio 1:20), as well as with water, absolute ethanol, or 50% (v/v) ethanol under
the same conditions. The qualitative analysis of extracts was conducted by both HPLC and LC-MS. The content of the identified
marker compounds in the extracts, i.e. rosmarinic acid (RA) and clinopodic acid O (CAO), was determined by external
calibration using RA as the standard compound. Cytotoxicity of the aqueous DESs against human pancreatic adenocarcinoma
cells AsPC-1 was tested at four concentration levels (5-25%), after 48 h of incubation and propidium iodide staining.
The analysis on Guava® easyCyte 12HT Benchtop flow microcapillary cytometer, was performed afterwards, using InCyte®
software package.
RESULTS:
Upon freeze-drying, mixtures had glassy appearance and transformed into liquids after mild heating. All obtained DESs were
highly viscous, and therefore mixed with water. Qualitative LC-MS analysis of 50% ethanol extract revealed the presence of
phenolic acids, flavonoids, and jasmonic acid derivatives. Among phenolic acids, the dominant compounds were caffeic
acid oligomers RA and CAO. Among conventional solvents, 50% ethanol was better extracting agent than absolute ethanol
or water for both RA (88.2 μg/mL) and CAO (116.8 μg/mL). Water extract was also abundant with CAO (106.7 μg/mL), but
contained moderate amount of RA (21.7 μg/mL). It is noteworthy to mention that the extraction with ethanol resulted in
very low yield of both phenolics, with CAO concentration, being even below detection limit in the absolute ethanol extract.
Concentration of RA in the tested DES extracts was higher than corresponding one in the water extract, but lower than in
the 50% ethanol extract, and varied in the range from 61.6 μg/mL (in Pro-fructose 1:1 extract) to 85.6 μg/mL (Pro-glucose 1:1
extract). The extraction of CAO, with six out of seven aqueous DESs, was more efficient than with 50% ethanol, resulting in
CAO concentration range from 119.6 μg/mL, in Pro-sucrose 3:2 extract, to 172.4 μg/mL, in Pro-glucose 1:1 extract. Pro-fructose
1:1 extract had the lowest content of CAO (86.7 μg/mL) among the tested DESs. At the lowest tested concentration (5%),
aqueous DESs did not significantly affect survival of AsPC-1 cells in comparison to the untreated cells (83.1-90.0% and 86.6%
of cells remained viable, respectively). Both proline-glucose DESs demonstrated the lowest toxicity. However, at the highest
concentration (25%) all aqueous DESs caused death of more than 70% of AsPC-1 cells. CONCLUSIONS:
The obtained results indicate that proline and sugar/sugar alcohol based deep eutectic solvents are good extracting agents
for phenolic compounds, especially for higher caffeic acid oligomers such as clinopodic acid O. Additionally, low cytotoxicity
of tested DESs is a good starting predictor of their safety and potential usage.",
publisher = "University Prince of Songkla, Thailand, University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts",
title = "Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii",
pages = "31-33",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5103"
}
Arsenijević, J., Kovačević, N., Drobac, M., Ražić, S., Emhemmed, F., Muller, C., Marcic, C.,& Marchioni, E.. (2021). Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
University Prince of Songkla, Thailand., 31-33.
https://hdl.handle.net/21.15107/rcub_farfar_5103
Arsenijević J, Kovačević N, Drobac M, Ražić S, Emhemmed F, Muller C, Marcic C, Marchioni E. Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts. 2021;:31-33.
https://hdl.handle.net/21.15107/rcub_farfar_5103 .
Arsenijević, Jelena, Kovačević, Nada, Drobac, Milica, Ražić, Slavica, Emhemmed, Fathi, Muller, Christian, Marcic, Christophe, Marchioni, Eric, "Proline-based deep eutectic solvents as greener alternative for obtaining polyphenol rich extracts of Satureja kitaibelii" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts (2021):31-33,
https://hdl.handle.net/21.15107/rcub_farfar_5103 .

Polyphenolic composition of commercial samples of rtanj tea

Arsenijević, Jelena; Marčetić, Mirjana; Drobac, Milica; Slavkovska, Violeta; Kovačević, Nada

(Savez farmaceutskih udruženja Srbije (SFUS), 2021)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Marčetić, Mirjana
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4669
AB  - Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions.
AB  - Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Polyphenolic composition of commercial samples of rtanj tea
T1  - Polifenolni sastav komercijalnih uzoraka rtanjskog čaja
VL  - 71
IS  - 5 suplement
SP  - S54
EP  - S55
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4669
ER  - 
@conference{
author = "Arsenijević, Jelena and Marčetić, Mirjana and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada",
year = "2021",
abstract = "Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions., Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Polyphenolic composition of commercial samples of rtanj tea, Polifenolni sastav komercijalnih uzoraka rtanjskog čaja",
volume = "71",
number = "5 suplement",
pages = "S54-S55",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4669"
}
Arsenijević, J., Marčetić, M., Drobac, M., Slavkovska, V.,& Kovačević, N.. (2021). Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669
Arsenijević J, Marčetić M, Drobac M, Slavkovska V, Kovačević N. Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju. 2021;71(5 suplement):S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669 .
Arsenijević, Jelena, Marčetić, Mirjana, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, "Polyphenolic composition of commercial samples of rtanj tea" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S54-S55,
https://hdl.handle.net/21.15107/rcub_farfar_4669 .

2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts

Kovačević, Nada; Miljković, Branislava; Parojčić, Jelena

(Association of the Chemical Engineers of Serbia, 2021)

TY  - JOUR
AU  - Kovačević, Nada
AU  - Miljković, Branislava
AU  - Parojčić, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4057
AB  - From the idea of a pharmaceutical product, through the development of materials and technologies for their production, analysis, and control, application in different patient populations, to the proper disposal and destroying of pharmaceutical waste, everything is connected and has an impact on the soil, water, and air, as well as to all living beings on this planet. Intending to invite the colleagues to consider how their pharmacy practice, science, and education can impact nature and global environmental trends, Pharmaceutical Association of Serbia has organized the 2nd SFUS scientific symposium, entitled Pharmacy and the Nature - Complex Relations and Mutual Impacts on 28th October 2021, in the conference center of the Science and Technology Park Belgrade, with the presence of more than 60 participants. Within the Symposium, the introductory lecture was held by Prof. Dr. Nada Kovačević, along with eight invited lectures, three oral presentations, and thirty-two poster presentations.
AB  - Od ideje za farmaceutski proizvod, preko razvoja materijala i tehnologija za njihovu proizvodnju, analizu i kontrolu, primene kod različitih populacija pacijenata, do pravilnog odlaganja i uništavanja iskorišćenog i neiskorišćenog materijala i proizvoda, sve je povezano i ima uticaj na zemlju, vodu i vazduh, kao i na sva živa bića na ovoj planeti. Sa ciljem da se bolje razmotri na koji način aktivnosti u okviru farmaceutske struke, nauke i obrazovanja mogu da utiču na prirodu i glabalna ekološka kretanja, održan je 2. naučni simpozijum pod naslovom Farmacija i priroda – kompleksne relacije i međusobni uticaji. Simpozijum je organizovao Savez farmaceutskih udruženja Srbije i održan je 28. oktobra 2021. godine u konferencijskom centru Naučno-tehnološkog parka Beograd, uz prisutvo više od 60 učesnika. U okviru Simpozijuma je održano jedno uvodno predavanje, osam predavanja po pozivu, tri usmena saopštenja i prikazane su trideset i dve poster prezentacije.
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - 2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts
T1  - Drugi naučni simpozijum Saveza farmaceutskih udruženja Srbije: Farmacija i priroda - kompleksne relacije i međusobni uticaji
VL  - 75
IS  - 6
SP  - 373
EP  - 377
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4057
ER  - 
@article{
author = "Kovačević, Nada and Miljković, Branislava and Parojčić, Jelena",
year = "2021",
abstract = "From the idea of a pharmaceutical product, through the development of materials and technologies for their production, analysis, and control, application in different patient populations, to the proper disposal and destroying of pharmaceutical waste, everything is connected and has an impact on the soil, water, and air, as well as to all living beings on this planet. Intending to invite the colleagues to consider how their pharmacy practice, science, and education can impact nature and global environmental trends, Pharmaceutical Association of Serbia has organized the 2nd SFUS scientific symposium, entitled Pharmacy and the Nature - Complex Relations and Mutual Impacts on 28th October 2021, in the conference center of the Science and Technology Park Belgrade, with the presence of more than 60 participants. Within the Symposium, the introductory lecture was held by Prof. Dr. Nada Kovačević, along with eight invited lectures, three oral presentations, and thirty-two poster presentations., Od ideje za farmaceutski proizvod, preko razvoja materijala i tehnologija za njihovu proizvodnju, analizu i kontrolu, primene kod različitih populacija pacijenata, do pravilnog odlaganja i uništavanja iskorišćenog i neiskorišćenog materijala i proizvoda, sve je povezano i ima uticaj na zemlju, vodu i vazduh, kao i na sva živa bića na ovoj planeti. Sa ciljem da se bolje razmotri na koji način aktivnosti u okviru farmaceutske struke, nauke i obrazovanja mogu da utiču na prirodu i glabalna ekološka kretanja, održan je 2. naučni simpozijum pod naslovom Farmacija i priroda – kompleksne relacije i međusobni uticaji. Simpozijum je organizovao Savez farmaceutskih udruženja Srbije i održan je 28. oktobra 2021. godine u konferencijskom centru Naučno-tehnološkog parka Beograd, uz prisutvo više od 60 učesnika. U okviru Simpozijuma je održano jedno uvodno predavanje, osam predavanja po pozivu, tri usmena saopštenja i prikazane su trideset i dve poster prezentacije.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts, Drugi naučni simpozijum Saveza farmaceutskih udruženja Srbije: Farmacija i priroda - kompleksne relacije i međusobni uticaji",
volume = "75",
number = "6",
pages = "373-377",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4057"
}
Kovačević, N., Miljković, B.,& Parojčić, J.. (2021). 2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts. in Hemijska industrija
Association of the Chemical Engineers of Serbia., 75(6), 373-377.
https://hdl.handle.net/21.15107/rcub_farfar_4057
Kovačević N, Miljković B, Parojčić J. 2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts. in Hemijska industrija. 2021;75(6):373-377.
https://hdl.handle.net/21.15107/rcub_farfar_4057 .
Kovačević, Nada, Miljković, Branislava, Parojčić, Jelena, "2nd SFUS scientific symposium Pharmacy and the Nature-Complex Relations and Mutual Impacts" in Hemijska industrija, 75, no. 6 (2021):373-377,
https://hdl.handle.net/21.15107/rcub_farfar_4057 .

Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)

Ilić, Milan D.; Marčetić, Mirjana; Zlatković, Bojan K.; Lakušić, Branislava; Kovačević, Nada; Drobac, Milica

(Wiley-VHCA AG, 2020)

TY  - JOUR
AU  - Ilić, Milan D.
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan K.
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3533
AB  - Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.
PB  - Wiley-VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)
VL  - 17
IS  - 2
DO  - 10.1002/cbdv.201900544
ER  - 
@article{
author = "Ilić, Milan D. and Marčetić, Mirjana and Zlatković, Bojan K. and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2020",
abstract = "Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.",
publisher = "Wiley-VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)",
volume = "17",
number = "2",
doi = "10.1002/cbdv.201900544"
}
Ilić, M. D., Marčetić, M., Zlatković, B. K., Lakušić, B., Kovačević, N.,& Drobac, M.. (2020). Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity
Wiley-VHCA AG., 17(2).
https://doi.org/10.1002/cbdv.201900544
Ilić MD, Marčetić M, Zlatković BK, Lakušić B, Kovačević N, Drobac M. Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity. 2020;17(2).
doi:10.1002/cbdv.201900544 .
Ilić, Milan D., Marčetić, Mirjana, Zlatković, Bojan K., Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)" in Chemistry and Biodiversity, 17, no. 2 (2020),
https://doi.org/10.1002/cbdv.201900544 . .
1
6
2
6

Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia

Slavkovska, Violeta; Zlatković, Bojan; Lakušić, Branislava; Kovačević, Nada; Drobac, Milica

(Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 2019)

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5293
AB  - This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5293
ER  - 
@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2019",
abstract = "This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5293"
}
Slavkovska, V., Zlatković, B., Lakušić, B., Kovačević, N.,& Drobac, M.. (2019). Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293
Slavkovska V, Zlatković B, Lakušić B, Kovačević N, Drobac M. Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293 .
Slavkovska, Violeta, Zlatković, Bojan, Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):27-27,
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga

Kovačević, Nada

(Komora magistara farmacije Federacije Bosne i Hercegovine, 2019)

TY  - CONF
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5310
AB  - Pod endemitima (endemičnim vrstama) podrazumevamo žive stisteme (populacije, podvrste, vrste, rodove itd.) koji prirodno naseljavaju neko ograničeno, veće ili manje, geografsko područje, nasuprot kozmopolitskim vrstama, koje su široko rasprostranjene gotovo po čitavoj zemljinoj površini. ...
AB  - By endemics (endemic species) we mean living systems (populations, subspecies, species, genera, etc.) that naturally inhabit a limited, larger or smaller geographical area, as opposed to cosmopolitan species, which are widespread almost throughout the earth. The current distribution of plants is not only conditioned by the environmental conditions of an area, but is also a consequence of their geological past. ...
PB  - Komora magistara farmacije Federacije Bosne i Hercegovine
PB  - Farmaceutsko društvo Republike Srpske
C3  - 4.kongres farmaceuta BiH sa međunarodnim učešćem, 10-13.10.2019., Sarajevo, Knjiga sažetaka
T1  - Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga
T1  - Endemic plant species – potential source of new natural substances and herbal drugs
SP  - 198
EP  - 199
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5310
ER  - 
@conference{
author = "Kovačević, Nada",
year = "2019",
abstract = "Pod endemitima (endemičnim vrstama) podrazumevamo žive stisteme (populacije, podvrste, vrste, rodove itd.) koji prirodno naseljavaju neko ograničeno, veće ili manje, geografsko područje, nasuprot kozmopolitskim vrstama, koje su široko rasprostranjene gotovo po čitavoj zemljinoj površini. ..., By endemics (endemic species) we mean living systems (populations, subspecies, species, genera, etc.) that naturally inhabit a limited, larger or smaller geographical area, as opposed to cosmopolitan species, which are widespread almost throughout the earth. The current distribution of plants is not only conditioned by the environmental conditions of an area, but is also a consequence of their geological past. ...",
publisher = "Komora magistara farmacije Federacije Bosne i Hercegovine, Farmaceutsko društvo Republike Srpske",
journal = "4.kongres farmaceuta BiH sa međunarodnim učešćem, 10-13.10.2019., Sarajevo, Knjiga sažetaka",
title = "Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga, Endemic plant species – potential source of new natural substances and herbal drugs",
pages = "198-199",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5310"
}
Kovačević, N.. (2019). Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga. in 4.kongres farmaceuta BiH sa međunarodnim učešćem, 10-13.10.2019., Sarajevo, Knjiga sažetaka
Komora magistara farmacije Federacije Bosne i Hercegovine., 198-199.
https://hdl.handle.net/21.15107/rcub_farfar_5310
Kovačević N. Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga. in 4.kongres farmaceuta BiH sa međunarodnim učešćem, 10-13.10.2019., Sarajevo, Knjiga sažetaka. 2019;:198-199.
https://hdl.handle.net/21.15107/rcub_farfar_5310 .
Kovačević, Nada, "Endemične biljke – potencijalni izvori novih prirodnih jedinjenja i novih biljnih droga" in 4.kongres farmaceuta BiH sa međunarodnim učešćem, 10-13.10.2019., Sarajevo, Knjiga sažetaka (2019):198-199,
https://hdl.handle.net/21.15107/rcub_farfar_5310 .

Antimicrobial activity of eight Geranium L. species extracts

Ilić, Milan; Drobac, Milica; Marčetić, Mirjana; Milenković, Marina; Zlatković, Bojan; Kovačević, Nada

(Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 2019)

TY  - CONF
AU  - Ilić, Milan
AU  - Drobac, Milica
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5290
AB  - The antimicrobial activity of methanol extracts of aerial parts of 8 Geranium
L. species (G. macrorrhizum L., G. phaeum L., G. sanguineum L., G. robertianum L.,
G. palustre L., G. pyrenaicum Burm. f., G. columbinum L. and G. lucidum L.) was
tested against 8 standard strains (Staphylococcus aureus, Enterococcus faecalis,
Bacillus subtilis, Escherichia coli, Klebsiella pneumaniae, Pseudomonas aeruginosa,
Salmonella abony and Candida albicans) as well as 10 clinical isolates of E. coli.
Also, total phenolics and tannins were quantified using spectrophotometric Folin-
Ciocalteu method. The extracts exhibited antibacterial and anticandidal activity with
minimal inhibitory concentrations (MICs) between 12.5 and 200 μg/ml. E. faecalis
was most sensitive strain (MIC 12.5-50 μg/ml), especially to G. columbinum and G.
phaeum extracts (MICs 12.5 μg/ml). The determined amounts of total phenolics and
tannins were in the range of 170-534 and 38-386 mg GAE/g of dry extract,
respectively, with the highest content in G. sanguineum extract. However, the
antimicrobial activity was not related to total polyphenolics or tannin content and
further investigation in regard with phenolic profile is needed.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Antimicrobial activity of eight Geranium L. species extracts
SP  - 171
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5290
ER  - 
@conference{
author = "Ilić, Milan and Drobac, Milica and Marčetić, Mirjana and Milenković, Marina and Zlatković, Bojan and Kovačević, Nada",
year = "2019",
abstract = "The antimicrobial activity of methanol extracts of aerial parts of 8 Geranium
L. species (G. macrorrhizum L., G. phaeum L., G. sanguineum L., G. robertianum L.,
G. palustre L., G. pyrenaicum Burm. f., G. columbinum L. and G. lucidum L.) was
tested against 8 standard strains (Staphylococcus aureus, Enterococcus faecalis,
Bacillus subtilis, Escherichia coli, Klebsiella pneumaniae, Pseudomonas aeruginosa,
Salmonella abony and Candida albicans) as well as 10 clinical isolates of E. coli.
Also, total phenolics and tannins were quantified using spectrophotometric Folin-
Ciocalteu method. The extracts exhibited antibacterial and anticandidal activity with
minimal inhibitory concentrations (MICs) between 12.5 and 200 μg/ml. E. faecalis
was most sensitive strain (MIC 12.5-50 μg/ml), especially to G. columbinum and G.
phaeum extracts (MICs 12.5 μg/ml). The determined amounts of total phenolics and
tannins were in the range of 170-534 and 38-386 mg GAE/g of dry extract,
respectively, with the highest content in G. sanguineum extract. However, the
antimicrobial activity was not related to total polyphenolics or tannin content and
further investigation in regard with phenolic profile is needed.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Antimicrobial activity of eight Geranium L. species extracts",
pages = "171-171",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5290"
}
Ilić, M., Drobac, M., Marčetić, M., Milenković, M., Zlatković, B.,& Kovačević, N.. (2019). Antimicrobial activity of eight Geranium L. species extracts. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 171-171.
https://hdl.handle.net/21.15107/rcub_farfar_5290
Ilić M, Drobac M, Marčetić M, Milenković M, Zlatković B, Kovačević N. Antimicrobial activity of eight Geranium L. species extracts. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:171-171.
https://hdl.handle.net/21.15107/rcub_farfar_5290 .
Ilić, Milan, Drobac, Milica, Marčetić, Mirjana, Milenković, Marina, Zlatković, Bojan, Kovačević, Nada, "Antimicrobial activity of eight Geranium L. species extracts" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):171-171,
https://hdl.handle.net/21.15107/rcub_farfar_5290 .

Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans

Marčetić, Mirjana; Božić, Dragana; Milenković, Marina; Kovačević, Mirjana; Kovačević, Nada

(Pharmaceutical Association of Serbia, 2019)

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Mirjana
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3664
AB  - Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans.
AB  - The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans
T1  - Antifungalna aktivnost etarskog ulja Seseli rigidum Waldst. & Kit. (Apiaceae) na rast izolata Candida albicans
VL  - 69
IS  - 2
SP  - 67
EP  - 79
DO  - 10.5937/arhfarm1902067x
ER  - 
@article{
author = "Marčetić, Mirjana and Božić, Dragana and Milenković, Marina and Kovačević, Mirjana and Kovačević, Nada",
year = "2019",
abstract = "Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans., The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans, Antifungalna aktivnost etarskog ulja Seseli rigidum Waldst. & Kit. (Apiaceae) na rast izolata Candida albicans",
volume = "69",
number = "2",
pages = "67-79",
doi = "10.5937/arhfarm1902067x"
}
Marčetić, M., Božić, D., Milenković, M., Kovačević, M.,& Kovačević, N.. (2019). Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 69(2), 67-79.
https://doi.org/10.5937/arhfarm1902067x
Marčetić M, Božić D, Milenković M, Kovačević M, Kovačević N. Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans. in Arhiv za farmaciju. 2019;69(2):67-79.
doi:10.5937/arhfarm1902067x .
Marčetić, Mirjana, Božić, Dragana, Milenković, Marina, Kovačević, Mirjana, Kovačević, Nada, "Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans" in Arhiv za farmaciju, 69, no. 2 (2019):67-79,
https://doi.org/10.5937/arhfarm1902067x . .

Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau

Ilić, Milan; Marčetić, Mirjana; Zlatković, Bojan; Lakušić, Branislava; Drobac, Milica; Kovačević, Nada

(Institute of Botany and Botanical Garden Jevremovac, Belgrade, 2018)

TY  - CONF
AU  - Ilić, Milan
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Drobac, Milica
AU  - Kovačević, Nada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5256
AB  - The Vlasina plateau shows significant floristic diversity counting 956 species of which 91 may be categorized as endangered
in the flora of Serbia. The genus Geranium is present in the
flora of this region with few species and they are the subject
of our research. The chemical composition of volatile compounds of aerial and underground parts of eight Geranium L.
species (G. macrorrhizum L., G. phaeum L., G. sanguineum L.,
G. robertianum L., G. palustre L., G. pyrenaicum Burm. f., G.
columbinum L. and G. lucidum L.) collected in flowering stage
from Vlasina plateau (Serbia) was examined. The samples of
volatile fraction were obtained by steam distillation and analyzed using GC-FID and GC-MS. The cluster analysis revealed
the separation of volatile compounds of Geranium species to
two clusters. The aerial and underground parts volatiles of G.
macrorrhizum were separated from the other species, based
on the high sequiterpenes content (92.3% and 94.6%), mostly
germacrone (73.3%) in the aerial and δ-guaiene (76.8%) in
the underground parts. The volatile fractions of other species
were mainly composed of sesquiterpenes (10.8-61.8%), diterpenes (12.9-43.0%) and fatty acids and their derivatives (6.6-
21.6%). The exception was the fraction of the underground
parts of G. phaeum which was predominantly made by fatty
acids and their derivatives (76.6%). In the volatile fraction of
G. palustre aerial parts β-selinene (18.6%) and (E)-caryophyllene (15.7%) prevailed. Hexadecanoic acid (15.4%), germacrene D (15.4%), (E)-caryophyllene (10.6%), phytol (10.4%)
and caryophyllene oxide (10.2%) were the most abundant
compounds in volatile fraction of G. pyrenaicum aerial parts,
while phytol (13.2%) and (E)-caryophyllene (9.5%) in the volatile fraction of G. lucidum aerial parts. Hexadecanoic acid
and phytol were dominant compounds in the volatile fraction
of aerial parts of G. sanguineum (21.1% and 17.8%), G. robertianum (12.5% and 19.3%), G. phaeum (7.3% and 41.4%) and
G. columbinum (14.5% and 29.5%). Volatiles of aerial parts of
G. robertianum were also characterized by (E)-caryophylene
(8.0%) and caryophyllene oxide (7.4%), while the volatiles of
G. sanguineum contained β-bisabolene (5.2%). The volatiles
of G. palustre aerial parts have been studied for the first time.
The chemical characterization of volatile compounds of Geranium species may possibly have chemotaxonomic importance
and is significant for further research of Vlasina plateau native
flora.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau
VL  - 42
IS  - 1 supplement
SP  - 141
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5256
ER  - 
@conference{
author = "Ilić, Milan and Marčetić, Mirjana and Zlatković, Bojan and Lakušić, Branislava and Drobac, Milica and Kovačević, Nada",
year = "2018",
abstract = "The Vlasina plateau shows significant floristic diversity counting 956 species of which 91 may be categorized as endangered
in the flora of Serbia. The genus Geranium is present in the
flora of this region with few species and they are the subject
of our research. The chemical composition of volatile compounds of aerial and underground parts of eight Geranium L.
species (G. macrorrhizum L., G. phaeum L., G. sanguineum L.,
G. robertianum L., G. palustre L., G. pyrenaicum Burm. f., G.
columbinum L. and G. lucidum L.) collected in flowering stage
from Vlasina plateau (Serbia) was examined. The samples of
volatile fraction were obtained by steam distillation and analyzed using GC-FID and GC-MS. The cluster analysis revealed
the separation of volatile compounds of Geranium species to
two clusters. The aerial and underground parts volatiles of G.
macrorrhizum were separated from the other species, based
on the high sequiterpenes content (92.3% and 94.6%), mostly
germacrone (73.3%) in the aerial and δ-guaiene (76.8%) in
the underground parts. The volatile fractions of other species
were mainly composed of sesquiterpenes (10.8-61.8%), diterpenes (12.9-43.0%) and fatty acids and their derivatives (6.6-
21.6%). The exception was the fraction of the underground
parts of G. phaeum which was predominantly made by fatty
acids and their derivatives (76.6%). In the volatile fraction of
G. palustre aerial parts β-selinene (18.6%) and (E)-caryophyllene (15.7%) prevailed. Hexadecanoic acid (15.4%), germacrene D (15.4%), (E)-caryophyllene (10.6%), phytol (10.4%)
and caryophyllene oxide (10.2%) were the most abundant
compounds in volatile fraction of G. pyrenaicum aerial parts,
while phytol (13.2%) and (E)-caryophyllene (9.5%) in the volatile fraction of G. lucidum aerial parts. Hexadecanoic acid
and phytol were dominant compounds in the volatile fraction
of aerial parts of G. sanguineum (21.1% and 17.8%), G. robertianum (12.5% and 19.3%), G. phaeum (7.3% and 41.4%) and
G. columbinum (14.5% and 29.5%). Volatiles of aerial parts of
G. robertianum were also characterized by (E)-caryophylene
(8.0%) and caryophyllene oxide (7.4%), while the volatiles of
G. sanguineum contained β-bisabolene (5.2%). The volatiles
of G. palustre aerial parts have been studied for the first time.
The chemical characterization of volatile compounds of Geranium species may possibly have chemotaxonomic importance
and is significant for further research of Vlasina plateau native
flora.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau",
volume = "42",
number = "1 supplement",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5256"
}
Ilić, M., Marčetić, M., Zlatković, B., Lakušić, B., Drobac, M.,& Kovačević, N.. (2018). Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 141-141.
https://hdl.handle.net/21.15107/rcub_farfar_5256
Ilić M, Marčetić M, Zlatković B, Lakušić B, Drobac M, Kovačević N. Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):141-141.
https://hdl.handle.net/21.15107/rcub_farfar_5256 .
Ilić, Milan, Marčetić, Mirjana, Zlatković, Bojan, Lakušić, Branislava, Drobac, Milica, Kovačević, Nada, "Chemical composition of volatile compounds of eight Geranium L. species from Vlasina plateau" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):141-141,
https://hdl.handle.net/21.15107/rcub_farfar_5256 .

Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)

Arsenijević, Jelena; Drobac, Milica; Slavkovska, Violeta; Kovačević, Nada; Lakušić, Branislava

(Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd, 2018)

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
AU  - Lakušić, Branislava
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3053
AB  - Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)
VL  - 42
IS  - 2
SP  - 231
EP  - 239
DO  - 10.5281/zenodo.1468339
ER  - 
@article{
author = "Arsenijević, Jelena and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada and Lakušić, Branislava",
year = "2018",
abstract = "Frangula rupestris and F. alnus are the only two species of the genus Frangula in the flora of the Balkan Peninsula. Frangula alnus is well-known for anthranoid content, and its stem bark and fruits are widely used as laxatives. Data on anatomy, plant metabolites, and potential use of F. rupestris are scarce. In this work we analysed anatomy of the stem and leaves and performed phytochemical screening of the bark and leaves of F. rupestris. Specific anatomical characteristics of the stem include the presence of large mucilage cavities in the bark and pith, as well as numerous parenchyma cells containing solitary or cluster crystals of calcium oxalate. The majority of leaf epidermal cells are filled with mucilage. In the main leaf vein there is parenchyma with numerous mucilage cavities and solitary or cluster crystals of calcium oxalate. The levels of flavonoids, total phenolics, and tannins in bark and leaves of plants from two localities were determined by spectrophotometric methods, and the results were compared with those obtained for bark of F. alnus. Bark and leaves of F. rupestris contained 2.68-3.03% and 2.22-3.77% total phenolics, 1.70-2.10% and 0.57-1.54% tannins, and 0.12-0.36% and 0.57-0.99% flavonoids, respectively. The conducted HPLC and LC-MS analyses of hydromethanol extracts of bark and leaves of F. rupestris revealed the presence of flavonols. The dominant compound in all extracts was hyperoside and its content, determined by HPLC, ranged from 30.40 to 82.03 mg/g. Swelling indices determined for 0.5 g of plant material of F. rupestris bark and leaves (5.8-11.4 and 5.8-13.8) were higher than that of the bark of F. alnus (4.4) and greater than those of the mucilaginous drugs Althaeae folium and Althaeae radix (4.7-4.8). The high swelling indices of F. rupestris bark and (especially) leaves suggest their potential use as bulk-forming laxatives. In addition, differences in the content of metabolites were observed in plants from different localities.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)",
volume = "42",
number = "2",
pages = "231-239",
doi = "10.5281/zenodo.1468339"
}
Arsenijević, J., Drobac, M., Slavkovska, V., Kovačević, N.,& Lakušić, B.. (2018). Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 42(2), 231-239.
https://doi.org/10.5281/zenodo.1468339
Arsenijević J, Drobac M, Slavkovska V, Kovačević N, Lakušić B. Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae). in Botanica Serbica. 2018;42(2):231-239.
doi:10.5281/zenodo.1468339 .
Arsenijević, Jelena, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, Lakušić, Branislava, "Anatomical analysis and phytochemical screening of Frangula rupestris (Scop.) Schur (Rhamnaceae)" in Botanica Serbica, 42, no. 2 (2018):231-239,
https://doi.org/10.5281/zenodo.1468339 . .
2
1
2

Vazorelaksantna aktivnost terpinolena

Suručić, Relja; Drakul, Dragana; Kovačević, Nada; Kundaković-Vasović, Tatjana

(Savez farmaceutskih udruženja Srbije (SFUS), 2018)

TY  - CONF
AU  - Suručić, Relja
AU  - Drakul, Dragana
AU  - Kovačević, Nada
AU  - Kundaković-Vasović, Tatjana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5282
AB  - Terpeni predstavljaju veoma važnu grupu hemijskih jedinjenja kako zbog
industrijske upotrebe, tako i zbog svojih značajnih bioloških efekata koji se mogu
iskoristiti u medicini. Jedna su od najbrojnijih grupa sekundarnih metabolita biljaka, a
naziv ove klase jedinjenja potiče od terpentina (terpentinskog ulja), tečnog proizvoda
destilacije oleorezina bora. Terpinolen (p‐menta‐2,4(8)‐dien) je bezbojna ili bledo žuto
obojena tečnost aromatičnog mirisa. Ovaj monociklični, monoterpenski alken je široko
zastupljen u biljnim tkivima različitih četinara, paškanata, konoplje, čajevca, listu
kurkume i peršuna, a čest je sastojak etarskog ulja Citrus, Mentha, Juniperus i Myristica
vrsta. Jedna je od glavnih komponenata etarskog ulja nadzemnih delova endemične
vrste Seseli gracile Waldst. & Kit. (6,1‐57,5 %). Terpinolen poseduje potvrđenu
antiradikalsku, antihiperalgezijsku i antiedematoznu aktivnost. S obzirom da su neka
strukturno slična monoterpenska jedinjenja pokazala vazorelaksantnu aktivnost, cilj
ovog istraživanja je da se istraži vazorelaksantni potencijal terpinolena.
U ovom istraživanju ispitivan je efekat standarda terpinolena (43905 Sigma‐
Aldrich) na izolovanoj renalnoj arteriji pacova Wistar soja. Korišćeni su prstenasti
segmenti renalne arterije dužine 3‐5 mm sa očuvanim endotelom. Arterijski segmenti
su postavljani u vodeno kupatilo u Krebs‐Ringerov rastvor, na 37C i aerisani
kontinuiranim dovođenjem smješe 95 % O2 i 5 % CO2. Kontrakcija krvnih sudova je
izazvana primjenom fenilefrina (10‐6 M). Integritet endotela je potvrđen farmakološki,
primjenom acetilholina (10‐6 M). Na stabilan tonus krvnog suda izazvan fenilefrinom su
dodavane rastuće koncentracije terpinolena, pripremljenog od standarda
razblaživanjem u 5 % karboksi‐metil celuloze (0,2 % ‐ 33,3 %, kumulativno). Svaka
sledeća koncentracija je dodavana tek nakon što se završi efekat prethodno
primjenjene koncentracije, tj. otprilike nakon 15 minuta od prethodne. U kontrolnoj
seriji eksperimenata je na isti način dodavan rastvarač (6 dodavanja kumulativno, sa
vremenskim razmakom od 15 minuta).
Utvrđeno je da terpinolen u kocentraciji od 11,1% dovodi do statistički značajne
vazorelaksacije u odnosu na rastvarač kao kontrolu (F=2,584, p<0,05; Two‐way
ANOVA). Naši rezultati opravdavaju nastavak ispitivanja potencijalne primene
terpinolena u terapiji kardiovaskularnih oboljenja.
AB  - Terpenes represent a very important group of chemical compounds because of
their industrial use, as well as medical use which is possible due to their significant
biological effects. They are one of the largest groups of secondary plant metabolites.
The name of this class of compounds comes from turpentine (oil of turpentine), a liquid
product of pine oleoresin distillation process.
Terpinolene (p‐menth‐2,4 (8)‐diene) is a colorless or pale yellow colored liquid
with aromatic odor. This monocyclic, monoterpenic alkene is widely represented in
plant tissues of various pines, pashkans, cannabis, tea tree, turmeric and parsley leaves,
and it is often a component of essential oils obtained from Citrus, Mentha, Juniperus and
Myristica species. It is one of the main components of the essential oil of aerial parts of
the endemic species Seseli gracile Waldst. & Kit. (6.1‐57.5%). Terpinolen possesses
confirmed antiradical, antihyperalgesic and antiedematous activity. Since some
structurally similar monoterpenic compounds have previously shown vasorelaxant
activity, the aim of this study was to investigate the vasorelaxant potential of
terpinolene.
In the present study, the effects of standard terpinolene (43905 Sigma‐Aldrich)
on the isolated renal artery of Wistar rats were investigated. Three to five mm long
arterial ring tissue segments with preserved endothelium were used. The arterial
segments were placed in water bath in the Krebs‐Ringer solution, at 37° C and
continuously aerated by 95% O2 and 5% CO2. Contractions of the blood vessels were
induced with 10‐6 M of phenylephrine. The endothelium integrity was confirmed
pharmacologically, by using the acetylcholine (10‐6M). After accomplishing tonic phase
of the contraction, increasing concentration of terpinolene (0.2 % ‐ 33.3 %,
cumulatively) in 5 % carboxymethyl cellulose (CMC) solution were added to the organ
bath. Each subsequent concentration was added only after the end of effect of the
previously applied concentration, i.e. approximately 15 minutes from the previous one.
In the control series of experiments, a solvent was added in the same manner.
It was found that terpinolene at the concentration of 11.1% exibits statistically
significant vasorelaxation in comparison to the solvent control (F = 2.584, p <0.05;
Two‐way ANOVA). Our results justify a continuation for the further studies of
terpinolene’s potential in the treatment of cardiovascular diseases.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Vazorelaksantna aktivnost terpinolena
T1  - Vasorelaxant activity of terpinolene
VL  - 68
IS  - 3
SP  - 706
EP  - 707
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5282
ER  - 
@conference{
author = "Suručić, Relja and Drakul, Dragana and Kovačević, Nada and Kundaković-Vasović, Tatjana",
year = "2018",
abstract = "Terpeni predstavljaju veoma važnu grupu hemijskih jedinjenja kako zbog
industrijske upotrebe, tako i zbog svojih značajnih bioloških efekata koji se mogu
iskoristiti u medicini. Jedna su od najbrojnijih grupa sekundarnih metabolita biljaka, a
naziv ove klase jedinjenja potiče od terpentina (terpentinskog ulja), tečnog proizvoda
destilacije oleorezina bora. Terpinolen (p‐menta‐2,4(8)‐dien) je bezbojna ili bledo žuto
obojena tečnost aromatičnog mirisa. Ovaj monociklični, monoterpenski alken je široko
zastupljen u biljnim tkivima različitih četinara, paškanata, konoplje, čajevca, listu
kurkume i peršuna, a čest je sastojak etarskog ulja Citrus, Mentha, Juniperus i Myristica
vrsta. Jedna je od glavnih komponenata etarskog ulja nadzemnih delova endemične
vrste Seseli gracile Waldst. & Kit. (6,1‐57,5 %). Terpinolen poseduje potvrđenu
antiradikalsku, antihiperalgezijsku i antiedematoznu aktivnost. S obzirom da su neka
strukturno slična monoterpenska jedinjenja pokazala vazorelaksantnu aktivnost, cilj
ovog istraživanja je da se istraži vazorelaksantni potencijal terpinolena.
U ovom istraživanju ispitivan je efekat standarda terpinolena (43905 Sigma‐
Aldrich) na izolovanoj renalnoj arteriji pacova Wistar soja. Korišćeni su prstenasti
segmenti renalne arterije dužine 3‐5 mm sa očuvanim endotelom. Arterijski segmenti
su postavljani u vodeno kupatilo u Krebs‐Ringerov rastvor, na 37C i aerisani
kontinuiranim dovođenjem smješe 95 % O2 i 5 % CO2. Kontrakcija krvnih sudova je
izazvana primjenom fenilefrina (10‐6 M). Integritet endotela je potvrđen farmakološki,
primjenom acetilholina (10‐6 M). Na stabilan tonus krvnog suda izazvan fenilefrinom su
dodavane rastuće koncentracije terpinolena, pripremljenog od standarda
razblaživanjem u 5 % karboksi‐metil celuloze (0,2 % ‐ 33,3 %, kumulativno). Svaka
sledeća koncentracija je dodavana tek nakon što se završi efekat prethodno
primjenjene koncentracije, tj. otprilike nakon 15 minuta od prethodne. U kontrolnoj
seriji eksperimenata je na isti način dodavan rastvarač (6 dodavanja kumulativno, sa
vremenskim razmakom od 15 minuta).
Utvrđeno je da terpinolen u kocentraciji od 11,1% dovodi do statistički značajne
vazorelaksacije u odnosu na rastvarač kao kontrolu (F=2,584, p<0,05; Two‐way
ANOVA). Naši rezultati opravdavaju nastavak ispitivanja potencijalne primene
terpinolena u terapiji kardiovaskularnih oboljenja., Terpenes represent a very important group of chemical compounds because of
their industrial use, as well as medical use which is possible due to their significant
biological effects. They are one of the largest groups of secondary plant metabolites.
The name of this class of compounds comes from turpentine (oil of turpentine), a liquid
product of pine oleoresin distillation process.
Terpinolene (p‐menth‐2,4 (8)‐diene) is a colorless or pale yellow colored liquid
with aromatic odor. This monocyclic, monoterpenic alkene is widely represented in
plant tissues of various pines, pashkans, cannabis, tea tree, turmeric and parsley leaves,
and it is often a component of essential oils obtained from Citrus, Mentha, Juniperus and
Myristica species. It is one of the main components of the essential oil of aerial parts of
the endemic species Seseli gracile Waldst. & Kit. (6.1‐57.5%). Terpinolen possesses
confirmed antiradical, antihyperalgesic and antiedematous activity. Since some
structurally similar monoterpenic compounds have previously shown vasorelaxant
activity, the aim of this study was to investigate the vasorelaxant potential of
terpinolene.
In the present study, the effects of standard terpinolene (43905 Sigma‐Aldrich)
on the isolated renal artery of Wistar rats were investigated. Three to five mm long
arterial ring tissue segments with preserved endothelium were used. The arterial
segments were placed in water bath in the Krebs‐Ringer solution, at 37° C and
continuously aerated by 95% O2 and 5% CO2. Contractions of the blood vessels were
induced with 10‐6 M of phenylephrine. The endothelium integrity was confirmed
pharmacologically, by using the acetylcholine (10‐6M). After accomplishing tonic phase
of the contraction, increasing concentration of terpinolene (0.2 % ‐ 33.3 %,
cumulatively) in 5 % carboxymethyl cellulose (CMC) solution were added to the organ
bath. Each subsequent concentration was added only after the end of effect of the
previously applied concentration, i.e. approximately 15 minutes from the previous one.
In the control series of experiments, a solvent was added in the same manner.
It was found that terpinolene at the concentration of 11.1% exibits statistically
significant vasorelaxation in comparison to the solvent control (F = 2.584, p <0.05;
Two‐way ANOVA). Our results justify a continuation for the further studies of
terpinolene’s potential in the treatment of cardiovascular diseases.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Vazorelaksantna aktivnost terpinolena, Vasorelaxant activity of terpinolene",
volume = "68",
number = "3",
pages = "706-707",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5282"
}
Suručić, R., Drakul, D., Kovačević, N.,& Kundaković-Vasović, T.. (2018). Vazorelaksantna aktivnost terpinolena. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 706-707.
https://hdl.handle.net/21.15107/rcub_farfar_5282
Suručić R, Drakul D, Kovačević N, Kundaković-Vasović T. Vazorelaksantna aktivnost terpinolena. in Arhiv za farmaciju. 2018;68(3):706-707.
https://hdl.handle.net/21.15107/rcub_farfar_5282 .
Suručić, Relja, Drakul, Dragana, Kovačević, Nada, Kundaković-Vasović, Tatjana, "Vazorelaksantna aktivnost terpinolena" in Arhiv za farmaciju, 68, no. 3 (2018):706-707,
https://hdl.handle.net/21.15107/rcub_farfar_5282 .

Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L.

Marčetić, Mirjana; Bošković, Nikola; Stojanović, Danilo; Kovačević, Nada

(Savez farmaceutskih udruženja Srbije (SFUS), 2018)

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Bošković, Nikola
AU  - Stojanović, Danilo
AU  - Kovačević, Nada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5283
AB  - Tradicionalno list matičnjaka, Melissae folium, koristi se za ublažavanje blagih
simptoma mentalnog stresa i olakšavanje uspavljivanja, kao i u simptomatskoj terapiji
blagih gastrointestinalnih poremećaja. Etarsko ulje matičnjaka je veoma cenjeno u
prehrambenoj i kozmetičkoj industriji. Cilj rada je bio ispitivanje makroskopskih i
mikroskopskih osobina uzoraka lista samoniklog i gajenog matičnjaka sa različitih
lokaliteta i komercijalno dostupnih čajeva, kao i ispitivanja sastava njihovih etarskih
ulja.
Za analizu etarskih ulja (16 uzoraka), dobijenih destilacijom vodenom parom,
primenjena je gasna hromatografija (GC‐FID) i gasna hromatografija sa masenom
spektrometrijom (GC‐MS). Klaster analizom ispitivane su razlike između etarskih ulja.
Makroskopske i mikroskopske odlike svih uzoraka su odgovarale monografiji
Melissae folium u Ph. Eur. 7.0, ali je u 2 od 5 komercijalnih uzoraka bio veći sadržaj
stranih materija. U svim ispitivanim etarskim uljima glavne komponenate su
predstavljali: geranial (15,58‐53,33%), neral (10,73‐36,64 %), kariofilen oksid (0,76‐
35,57 %), (E)‐kariofilen (tragovi‐16,72 %) i citronelal (0,00‐12,64 %). Sadržaj geraniala
i nerala u ispitivanim uzorcima je bio u opsegu vrednosti dobijenih u prethodnim
ispitivanjima.
Klaster analizom uzorci etarskog ulja su podeljeni na dva klastera. U prvom
klasteru su bila etarska ulja sa visokim sadržajem geraniala i nerala (31,87‐53,33% i
19,81‐36,64%): etarska ulja lista i herbe samoniklog i gajenog matičnjaka u različitim
fazama razvoja i sa različitih lokaliteta i dva komercijalna uzorka. U drugi klaster su
svrstana etarska ulja sa nižim sadržajem geraniala i nerala (15,58‐26,67 % i 10,73‐
15,48 %) i većim udelom kariofilen‐oksida (17,79‐35,57 %): tri komercijalna uzorka i
etarsko ulje herbe. List samoniklog matičnjaka u različitim fazama razvoja, sadrži visok
procenat geraniala i nerala u etarskom ulju, ne razlikuje se značajno od gajenih uzoraka
i može predstavljati izvor kvalitetne droge.
AB  - Traditional herbal remedies based on lemon balm leaf, Melissae folium are used
for relief of mild symptoms of mental stress, to aid sleep and for symptomatic
treatment of mild gastrointestinal complaints. The essential oil of lemon balm is very
appreciated in food and cosmetic industry. The aim was to examine the macroscopic
and microscopic characteristics of lemon balm leaves of cultivated and wild plants from
different localities, commercially available tea samples and to determinate the
composition of their essential oils. The essential oils obtained by hydrodistillation (16
samples), were analysed by gas chromatography (GC‐FID) and gas chromatography
with mass spectrometry (GC‐MS). The differences between essential oils were
examined by cluster analysis.
Macroscopic and microscopic characteristics corresponded to the monograph
Melissae folium in Ph. Eur. 7.0, while 2 of 5 commercial samples contained higher
content of foreign matter.
The main components in investigated essential oils were: geranial (15.58‐53.33
%), neral (10.73‐36.64 %), caryophyllene oxide (0.76‐35.57 %), (E)‐caryophyllene
(traces‐16.72 %) and citronellal (0.00‐12.64 %). The content of geranial and neral was
in the range of values obtained in previous investigations.
Cluster analysis divided samples of essential oils into two clusters. Essential oils
with high geranial and neral content (31.87‐53.33 % and 19.81‐36.64 %) were in the
first cluster: essential oils of leaf and aerial parts of wild and cultivated lemon balm
from different development phases and localities and two commercial samples. The
essential oils with lower geranial and neral content (15.58‐26.67 % and 10.73‐15.48 %)
and higher caryophyllene oxide (17.79‐35.57 %) were separated to the second cluster:
three commercial samples and essential oil of aerial parts.The wild lemon balm leaf in
different phases of development contains essential oil with high amounts of geranial
and neral. It is not significantly different from cultivated samples and could represent a
source of quality herbal substance.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L.
T1  - Investigation of macroscopic and microscopic characteristics and essential oil of the lemon balm leaf, Melissa officinalis L.
VL  - 68
IS  - 3
SP  - 698
EP  - 699
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5283
ER  - 
@conference{
author = "Marčetić, Mirjana and Bošković, Nikola and Stojanović, Danilo and Kovačević, Nada",
year = "2018",
abstract = "Tradicionalno list matičnjaka, Melissae folium, koristi se za ublažavanje blagih
simptoma mentalnog stresa i olakšavanje uspavljivanja, kao i u simptomatskoj terapiji
blagih gastrointestinalnih poremećaja. Etarsko ulje matičnjaka je veoma cenjeno u
prehrambenoj i kozmetičkoj industriji. Cilj rada je bio ispitivanje makroskopskih i
mikroskopskih osobina uzoraka lista samoniklog i gajenog matičnjaka sa različitih
lokaliteta i komercijalno dostupnih čajeva, kao i ispitivanja sastava njihovih etarskih
ulja.
Za analizu etarskih ulja (16 uzoraka), dobijenih destilacijom vodenom parom,
primenjena je gasna hromatografija (GC‐FID) i gasna hromatografija sa masenom
spektrometrijom (GC‐MS). Klaster analizom ispitivane su razlike između etarskih ulja.
Makroskopske i mikroskopske odlike svih uzoraka su odgovarale monografiji
Melissae folium u Ph. Eur. 7.0, ali je u 2 od 5 komercijalnih uzoraka bio veći sadržaj
stranih materija. U svim ispitivanim etarskim uljima glavne komponenate su
predstavljali: geranial (15,58‐53,33%), neral (10,73‐36,64 %), kariofilen oksid (0,76‐
35,57 %), (E)‐kariofilen (tragovi‐16,72 %) i citronelal (0,00‐12,64 %). Sadržaj geraniala
i nerala u ispitivanim uzorcima je bio u opsegu vrednosti dobijenih u prethodnim
ispitivanjima.
Klaster analizom uzorci etarskog ulja su podeljeni na dva klastera. U prvom
klasteru su bila etarska ulja sa visokim sadržajem geraniala i nerala (31,87‐53,33% i
19,81‐36,64%): etarska ulja lista i herbe samoniklog i gajenog matičnjaka u različitim
fazama razvoja i sa različitih lokaliteta i dva komercijalna uzorka. U drugi klaster su
svrstana etarska ulja sa nižim sadržajem geraniala i nerala (15,58‐26,67 % i 10,73‐
15,48 %) i većim udelom kariofilen‐oksida (17,79‐35,57 %): tri komercijalna uzorka i
etarsko ulje herbe. List samoniklog matičnjaka u različitim fazama razvoja, sadrži visok
procenat geraniala i nerala u etarskom ulju, ne razlikuje se značajno od gajenih uzoraka
i može predstavljati izvor kvalitetne droge., Traditional herbal remedies based on lemon balm leaf, Melissae folium are used
for relief of mild symptoms of mental stress, to aid sleep and for symptomatic
treatment of mild gastrointestinal complaints. The essential oil of lemon balm is very
appreciated in food and cosmetic industry. The aim was to examine the macroscopic
and microscopic characteristics of lemon balm leaves of cultivated and wild plants from
different localities, commercially available tea samples and to determinate the
composition of their essential oils. The essential oils obtained by hydrodistillation (16
samples), were analysed by gas chromatography (GC‐FID) and gas chromatography
with mass spectrometry (GC‐MS). The differences between essential oils were
examined by cluster analysis.
Macroscopic and microscopic characteristics corresponded to the monograph
Melissae folium in Ph. Eur. 7.0, while 2 of 5 commercial samples contained higher
content of foreign matter.
The main components in investigated essential oils were: geranial (15.58‐53.33
%), neral (10.73‐36.64 %), caryophyllene oxide (0.76‐35.57 %), (E)‐caryophyllene
(traces‐16.72 %) and citronellal (0.00‐12.64 %). The content of geranial and neral was
in the range of values obtained in previous investigations.
Cluster analysis divided samples of essential oils into two clusters. Essential oils
with high geranial and neral content (31.87‐53.33 % and 19.81‐36.64 %) were in the
first cluster: essential oils of leaf and aerial parts of wild and cultivated lemon balm
from different development phases and localities and two commercial samples. The
essential oils with lower geranial and neral content (15.58‐26.67 % and 10.73‐15.48 %)
and higher caryophyllene oxide (17.79‐35.57 %) were separated to the second cluster:
three commercial samples and essential oil of aerial parts.The wild lemon balm leaf in
different phases of development contains essential oil with high amounts of geranial
and neral. It is not significantly different from cultivated samples and could represent a
source of quality herbal substance.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L., Investigation of macroscopic and microscopic characteristics and essential oil of the lemon balm leaf, Melissa officinalis L.",
volume = "68",
number = "3",
pages = "698-699",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5283"
}
Marčetić, M., Bošković, N., Stojanović, D.,& Kovačević, N.. (2018). Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 698-699.
https://hdl.handle.net/21.15107/rcub_farfar_5283
Marčetić M, Bošković N, Stojanović D, Kovačević N. Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L.. in Arhiv za farmaciju. 2018;68(3):698-699.
https://hdl.handle.net/21.15107/rcub_farfar_5283 .
Marčetić, Mirjana, Bošković, Nikola, Stojanović, Danilo, Kovačević, Nada, "Ispitivanje makroskopskih, mikroskopskih osobina i etarskog ulja lista matičnjaka, Melissa officinalis L." in Arhiv za farmaciju, 68, no. 3 (2018):698-699,
https://hdl.handle.net/21.15107/rcub_farfar_5283 .

Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L.

Arsenijević, Jelena; Jovanović, Aleksandar; Jevtić, Stefan; Kovačević, Nada; Drobac, Milica

(Savez farmaceutskih udruženja Srbije (SFUS), 2018)

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Jovanović, Aleksandar
AU  - Jevtić, Stefan
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5279
AB  - Herba bosiljka se tradicionalno koristi kao karminativ, spazmolitik, blag sedativ i
laktagog. Etarsko ulje herbe bosiljka sadrži fenilpropanoid estragol, koji je kancerogen i
genotoksičan, a čija količina zavisi od hemotipa bosiljka. Količina estragola koja se
unosi putem biljnih lekovitih proizvoda, prema preporuci Evropske agencije za lekove
(EMA), ograničena je na 0,5 mg dnevno tokom 14 dana. Cilj rada bio je ispitivanje
hemijskog sastava etarskih ulja, sadržaja estragola u etarskim uljima i infuzima herbe
bosiljka, dostupne u Srbiji.
Ispitivani su komercijalni uzorci monokomponentnih čajeva i uzorci herbe
bosiljka gajenog u domaćinstvima u različitim delovima Srbije. Infuzi su pripremljeni
prelivanjem uzoraka ključalom vodom (2 g/150 ml) i ceđenjem nakon 15 min.
Izolovanje etarskog ulja iz herbe, kao i izolovanje estragola iz infuza izvršeno je
destilacijom vodenom parom u aparaturi po Klevendžeru. Analiza etarskih ulja i
destilata infuza izvršena je GC‐FID‐MS metodom. Sadržaj estragola u etarskim uljima i
infuzima određen je metodom eksternog standarda.
Sastav svih ispitivanih etarskih ulja odgovara evropskom hemotipu bosiljka,
kojeg karakteriše visok sadržaj linalola ili linalola i estragola. Sadržaj estragola u
ispitivanim etarskim uljima iznosio je 2,1‐565,4 mg/ml, a u infuzima 0,5‐11,3 μg/ml.
Primenom ispitivanih infuza prema preporuci Deutsche Arzneimittel‐Codex (DAC) (2
g/150 ml, tri puta dnevno), dnevni unos estragola iznosio bi 0,2‐5,1 mg.
Primenom infuza pojedinih uzoraka herbe bosiljka postoji mogućnost
prekoračenjа dozvoljenоg dnevnog unosa estragola. Dobijeni rezultati ukazuju na
značaj utvrđivanja sadržaja estragola u herbi bosiljka koja se koristi u obliku biljnih
lekovitih proizvoda.
AB  - Basil herb is traditionally used as carminative, spasmolytic, mild sedative and
lactagogue. Basil essential oil contains phenylpropanoide estragole, which is
carcinogenic and genotoxic, and its amount depends on Basil chemotype. The amount
of estragole administered through herbal medicinal products, according to the
recommendation of the European Medicines Agency (EMA), is limited to 0.5 mg daily
during 14 days. The aim of this work was examination of chemical composition of
essential oils, as well as determination of estragole content in essential oils and
infusions of Basil herb available in Serbia.
We have analyzed commercial samples of monocomponent Basil herbal teas and
samples of Basil herb cultivated in different parts of Serbia. Infusions were prepared by
steeping samples in boiling water (2 g/150 ml) for 15 min and subsequent filtering.
Isolation of the herb essential oil, as well as isolation of estragole from infusions was
carried out by hydrodistillation using a Clevenger‐type apparatus. Analyses of essential
oils and infusion distillates were performed using GC‐FID‐MS. The content of estragole
in essential oils and infusions was determined using external standard method.
Chemical composition of all investigated essential oils corresponds to European
chemotype of Basil that is characterized by high content of linalool or linalool and
estragole. The content of estragole in essential oils ranged from 2.1 to 565.4 mg/ml,
whereas infusions contained 0.5‐11.3 μg/ml of this compound. If the examined
infusions are administered according to the recommendation of Deutsche Arzneimittel‐
Codex (DAC) (2 g/150 ml, three times daily), the daily intake of estragole would be 0.2‐
5.1 mg.
By administration of infusions of some Basil herb samples, there is a possibility
of exceeding the permitted daily intake of estragole. The obtained results indicate the
importance of determining the content of estragole in Basil herb used in the form of
herbal medicinal products.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L.
T1  - The content of estragole in essential oil and infusion of basil herb, Ocimum basilicum L.
VL  - 68
IS  - 3
SP  - 712
EP  - 713
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5279
ER  - 
@conference{
author = "Arsenijević, Jelena and Jovanović, Aleksandar and Jevtić, Stefan and Kovačević, Nada and Drobac, Milica",
year = "2018",
abstract = "Herba bosiljka se tradicionalno koristi kao karminativ, spazmolitik, blag sedativ i
laktagog. Etarsko ulje herbe bosiljka sadrži fenilpropanoid estragol, koji je kancerogen i
genotoksičan, a čija količina zavisi od hemotipa bosiljka. Količina estragola koja se
unosi putem biljnih lekovitih proizvoda, prema preporuci Evropske agencije za lekove
(EMA), ograničena je na 0,5 mg dnevno tokom 14 dana. Cilj rada bio je ispitivanje
hemijskog sastava etarskih ulja, sadržaja estragola u etarskim uljima i infuzima herbe
bosiljka, dostupne u Srbiji.
Ispitivani su komercijalni uzorci monokomponentnih čajeva i uzorci herbe
bosiljka gajenog u domaćinstvima u različitim delovima Srbije. Infuzi su pripremljeni
prelivanjem uzoraka ključalom vodom (2 g/150 ml) i ceđenjem nakon 15 min.
Izolovanje etarskog ulja iz herbe, kao i izolovanje estragola iz infuza izvršeno je
destilacijom vodenom parom u aparaturi po Klevendžeru. Analiza etarskih ulja i
destilata infuza izvršena je GC‐FID‐MS metodom. Sadržaj estragola u etarskim uljima i
infuzima određen je metodom eksternog standarda.
Sastav svih ispitivanih etarskih ulja odgovara evropskom hemotipu bosiljka,
kojeg karakteriše visok sadržaj linalola ili linalola i estragola. Sadržaj estragola u
ispitivanim etarskim uljima iznosio je 2,1‐565,4 mg/ml, a u infuzima 0,5‐11,3 μg/ml.
Primenom ispitivanih infuza prema preporuci Deutsche Arzneimittel‐Codex (DAC) (2
g/150 ml, tri puta dnevno), dnevni unos estragola iznosio bi 0,2‐5,1 mg.
Primenom infuza pojedinih uzoraka herbe bosiljka postoji mogućnost
prekoračenjа dozvoljenоg dnevnog unosa estragola. Dobijeni rezultati ukazuju na
značaj utvrđivanja sadržaja estragola u herbi bosiljka koja se koristi u obliku biljnih
lekovitih proizvoda., Basil herb is traditionally used as carminative, spasmolytic, mild sedative and
lactagogue. Basil essential oil contains phenylpropanoide estragole, which is
carcinogenic and genotoxic, and its amount depends on Basil chemotype. The amount
of estragole administered through herbal medicinal products, according to the
recommendation of the European Medicines Agency (EMA), is limited to 0.5 mg daily
during 14 days. The aim of this work was examination of chemical composition of
essential oils, as well as determination of estragole content in essential oils and
infusions of Basil herb available in Serbia.
We have analyzed commercial samples of monocomponent Basil herbal teas and
samples of Basil herb cultivated in different parts of Serbia. Infusions were prepared by
steeping samples in boiling water (2 g/150 ml) for 15 min and subsequent filtering.
Isolation of the herb essential oil, as well as isolation of estragole from infusions was
carried out by hydrodistillation using a Clevenger‐type apparatus. Analyses of essential
oils and infusion distillates were performed using GC‐FID‐MS. The content of estragole
in essential oils and infusions was determined using external standard method.
Chemical composition of all investigated essential oils corresponds to European
chemotype of Basil that is characterized by high content of linalool or linalool and
estragole. The content of estragole in essential oils ranged from 2.1 to 565.4 mg/ml,
whereas infusions contained 0.5‐11.3 μg/ml of this compound. If the examined
infusions are administered according to the recommendation of Deutsche Arzneimittel‐
Codex (DAC) (2 g/150 ml, three times daily), the daily intake of estragole would be 0.2‐
5.1 mg.
By administration of infusions of some Basil herb samples, there is a possibility
of exceeding the permitted daily intake of estragole. The obtained results indicate the
importance of determining the content of estragole in Basil herb used in the form of
herbal medicinal products.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L., The content of estragole in essential oil and infusion of basil herb, Ocimum basilicum L.",
volume = "68",
number = "3",
pages = "712-713",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5279"
}
Arsenijević, J., Jovanović, A., Jevtić, S., Kovačević, N.,& Drobac, M.. (2018). Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 712-713.
https://hdl.handle.net/21.15107/rcub_farfar_5279
Arsenijević J, Jovanović A, Jevtić S, Kovačević N, Drobac M. Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L.. in Arhiv za farmaciju. 2018;68(3):712-713.
https://hdl.handle.net/21.15107/rcub_farfar_5279 .
Arsenijević, Jelena, Jovanović, Aleksandar, Jevtić, Stefan, Kovačević, Nada, Drobac, Milica, "Sadržaj estragola u etarskom ulju i infuzu herbe bosiljka, Ocimum basilicum L." in Arhiv za farmaciju, 68, no. 3 (2018):712-713,
https://hdl.handle.net/21.15107/rcub_farfar_5279 .

Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae)

Drobac, Milica; Zlatković, Bojan; Kovačević, Nada; Slavkovska, Violeta

(Savez farmaceutskih udruženja Srbije (SFUS), 2018)

TY  - CONF
AU  - Drobac, Milica
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
AU  - Slavkovska, Violeta
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5285
AB  - Koren vrste Pimpinella saxifraga L. se tradicionalno koristi kao ekspektorans,
bronhosekretolitik i antiflogistik. Cilj ovog rada je ispitivanje anatomske građe, sadržaja
i sastava etarskog ulja vegetativnih organa i ploda P. saxifraga. Biljni materijal je
sakupljan u jugoistočnoj Srbiji: Bojanine vode (Suva planina) i Ostrovica (Sićevačka
klisura). Anatomska analiza izvršena je na trajnim preparatima dobijenim standardnom
metodom pripreme za posmatranje pod svetlosnim mikroskopom. Etarska ulja su
izolovana iz herbe u cvetu, ploda, korena u fazi cvetanja i plodonošenja, destilacijom
vodenom parom i analizirana GC‐FID i GC‐MS metodama.
Rezultati anatomske analize su pokazali da je koren sekundarne građe a stablo
primarne sa zatvorenim kolateralnim sprovodnim snopićima. Listovi su dorziventralni,
hipoamfistomatski, lisna drška je sa lučno raspređenim sprovodnim snopićima, a
plodovi (merikarpi) su polukružnog oblika sa slabo izraženim rebrima, bez trihoma.
Retke, nežlezdane, višećelijske, uniserijatne trihome sa oštrim vrhom su uočene na
stablu, listovima i lisnoj dršci. Sekretorni kanali su brojni u svim organima.
Najviši sadržaj etarskog ulja određen je u plodu (1,48‐1,52%), dok su herba
(0,13‐0,21%) i koren (0,50‐0,53%) sadržali manju količinu ulja (v/m). Etarska ulja istih
organa sa različitih lokaliteta su sličnog kvalitativnog sastava sa manjim kvantitativnim
razlikama. U uljima herbe i ploda dominantna jedinjenja su β‐bisabolen (28,8‐76,0%) i
epoksi‐pseudoizoeugenil‐2‐metilbutirat (7,7‐21,8 %). Najzastupljenija jedinjenja u
uljima korena, u obe faze, su azuleni (31,4‐35,7%) i pregeijeren (13,7‐18,8%). U
ispitivanim etarskim uljima različitih organa P. saxifraga utvrđeno je prisustvo
trinorseskviterpena (azulena i geijerena) i fenilpropanoidnih jedinjenja
pseudoizoeugenil tipa, koja su karakteristični hemijski markeri za etarska ulja biljaka
ovog roda.
AB  - Root of Pimpinella saxifraga L. is traditionally used as expectorant,
bronchosecretolytic and antiphlogistic. The aim was investigation of anatomy, content
and composition of the essential oils of vegetative organs and fruits. Plant material was
collected in South‐Eastern Serbia: Bojanine vode (Mt. Suva planina) and Ostrovica
(Sićevo gorge). The anatomical analysis was conducted on permanent slides obtained
by standard method of preparation. The essential oils obtained by hydrodistillation
from flowering aerial parts, fruits and roots (from flowering and fruiting period) were
analysed by GC‐FID and GC‐MS.
The anatomical analysis revealed a secondary structure of root and primary
structure of stem with closed collateral vascular bundles. The leaves are dorsiventral,
hypoamphistomatic and petiole is with arched vascular bundles. The fruit (mericarp) is
semi‐circular and lightly ribbed in cross section without trichomes. Rare, nonglandular,
multicellular, uniseriate, cuneate trihomes were noted on the stem, leaves
and petiole. Secretory channels are numerous in all organs.
The highest content of essential oil was determined in the fruits (1.48‐1.52%),
while aerial parts (0.13‐0.21%) and roots (0.50‐0.53%) contained a lower amount of oil
(v/w). The oils from the same organs originated from different localities were similar in
qualitative composition with some quantitative differences. The most dominant
constituents in the oils from aerial parts and fruits were β‐bisabolene (28.8‐76.0%) and
epoxy‐pseudoisoeugenyl‐2‐methylbutyrate (7.7‐21.8 %), whereas those of the oils from
roots, in both stages, were azulenes (31.4‐35,7%) and pregeijerene (13.7‐18.8%).
Trinorsesquiterpenes (azulenes and geijerenes) and phenylpropanoid compounds of
the pseudoisoegenyl type, which are characteristic chemical markers of the essential
oils of the plants of this genus, were present in the all analyzed oils.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae)
T1  - Anatomy and chemical analysis of essential oil of Pimpinella saxifraga l. (Apiaceae)
VL  - 68
IS  - 3
SP  - 688
EP  - 689
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5285
ER  - 
@conference{
author = "Drobac, Milica and Zlatković, Bojan and Kovačević, Nada and Slavkovska, Violeta",
year = "2018",
abstract = "Koren vrste Pimpinella saxifraga L. se tradicionalno koristi kao ekspektorans,
bronhosekretolitik i antiflogistik. Cilj ovog rada je ispitivanje anatomske građe, sadržaja
i sastava etarskog ulja vegetativnih organa i ploda P. saxifraga. Biljni materijal je
sakupljan u jugoistočnoj Srbiji: Bojanine vode (Suva planina) i Ostrovica (Sićevačka
klisura). Anatomska analiza izvršena je na trajnim preparatima dobijenim standardnom
metodom pripreme za posmatranje pod svetlosnim mikroskopom. Etarska ulja su
izolovana iz herbe u cvetu, ploda, korena u fazi cvetanja i plodonošenja, destilacijom
vodenom parom i analizirana GC‐FID i GC‐MS metodama.
Rezultati anatomske analize su pokazali da je koren sekundarne građe a stablo
primarne sa zatvorenim kolateralnim sprovodnim snopićima. Listovi su dorziventralni,
hipoamfistomatski, lisna drška je sa lučno raspređenim sprovodnim snopićima, a
plodovi (merikarpi) su polukružnog oblika sa slabo izraženim rebrima, bez trihoma.
Retke, nežlezdane, višećelijske, uniserijatne trihome sa oštrim vrhom su uočene na
stablu, listovima i lisnoj dršci. Sekretorni kanali su brojni u svim organima.
Najviši sadržaj etarskog ulja određen je u plodu (1,48‐1,52%), dok su herba
(0,13‐0,21%) i koren (0,50‐0,53%) sadržali manju količinu ulja (v/m). Etarska ulja istih
organa sa različitih lokaliteta su sličnog kvalitativnog sastava sa manjim kvantitativnim
razlikama. U uljima herbe i ploda dominantna jedinjenja su β‐bisabolen (28,8‐76,0%) i
epoksi‐pseudoizoeugenil‐2‐metilbutirat (7,7‐21,8 %). Najzastupljenija jedinjenja u
uljima korena, u obe faze, su azuleni (31,4‐35,7%) i pregeijeren (13,7‐18,8%). U
ispitivanim etarskim uljima različitih organa P. saxifraga utvrđeno je prisustvo
trinorseskviterpena (azulena i geijerena) i fenilpropanoidnih jedinjenja
pseudoizoeugenil tipa, koja su karakteristični hemijski markeri za etarska ulja biljaka
ovog roda., Root of Pimpinella saxifraga L. is traditionally used as expectorant,
bronchosecretolytic and antiphlogistic. The aim was investigation of anatomy, content
and composition of the essential oils of vegetative organs and fruits. Plant material was
collected in South‐Eastern Serbia: Bojanine vode (Mt. Suva planina) and Ostrovica
(Sićevo gorge). The anatomical analysis was conducted on permanent slides obtained
by standard method of preparation. The essential oils obtained by hydrodistillation
from flowering aerial parts, fruits and roots (from flowering and fruiting period) were
analysed by GC‐FID and GC‐MS.
The anatomical analysis revealed a secondary structure of root and primary
structure of stem with closed collateral vascular bundles. The leaves are dorsiventral,
hypoamphistomatic and petiole is with arched vascular bundles. The fruit (mericarp) is
semi‐circular and lightly ribbed in cross section without trichomes. Rare, nonglandular,
multicellular, uniseriate, cuneate trihomes were noted on the stem, leaves
and petiole. Secretory channels are numerous in all organs.
The highest content of essential oil was determined in the fruits (1.48‐1.52%),
while aerial parts (0.13‐0.21%) and roots (0.50‐0.53%) contained a lower amount of oil
(v/w). The oils from the same organs originated from different localities were similar in
qualitative composition with some quantitative differences. The most dominant
constituents in the oils from aerial parts and fruits were β‐bisabolene (28.8‐76.0%) and
epoxy‐pseudoisoeugenyl‐2‐methylbutyrate (7.7‐21.8 %), whereas those of the oils from
roots, in both stages, were azulenes (31.4‐35,7%) and pregeijerene (13.7‐18.8%).
Trinorsesquiterpenes (azulenes and geijerenes) and phenylpropanoid compounds of
the pseudoisoegenyl type, which are characteristic chemical markers of the essential
oils of the plants of this genus, were present in the all analyzed oils.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae), Anatomy and chemical analysis of essential oil of Pimpinella saxifraga l. (Apiaceae)",
volume = "68",
number = "3",
pages = "688-689",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5285"
}
Drobac, M., Zlatković, B., Kovačević, N.,& Slavkovska, V.. (2018). Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 68(3), 688-689.
https://hdl.handle.net/21.15107/rcub_farfar_5285
Drobac M, Zlatković B, Kovačević N, Slavkovska V. Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae). in Arhiv za farmaciju. 2018;68(3):688-689.
https://hdl.handle.net/21.15107/rcub_farfar_5285 .
Drobac, Milica, Zlatković, Bojan, Kovačević, Nada, Slavkovska, Violeta, "Anatomska građa i hemijska analiza etarskog ulja Pimpinella saxifraga L. (Apiaceae)" in Arhiv za farmaciju, 68, no. 3 (2018):688-689,
https://hdl.handle.net/21.15107/rcub_farfar_5285 .

Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition

Slavkovska, Violeta; Zlatković, Bojan; Kovačević, Nada; Drobac, Milica

(Institute of Botany and Botanical Garden Jevremovac, Belgrade, 2018)

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5253
AB  - Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting
dry grasslands, limestone rocky areas and screes
of wide sense Mediterranean area, east European lowlands
and Caucasus. This study explores the anatomical structure,
content and composition of the essential oil of the vegetative
organs and fruit of this species. The plant material was collected
on two localities: the village Izvor (Bosilegrad, Serbia)
and Mt. Galičica (FYR Macedonia). The anatomical studies
were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope.
The essential oils obtained by hydrodistillation from roots
(collected during flowering and fruiting period), aerial parts
with inflorescences, and fruits, were analysed by GC-FID and
GC-MS. The anatomical analysis revealed a secondary structure
of root and primary structure of stem with closed collateral
vascular bundles. Leaves are isobilateral, amphistomatic;
petiole is with arched vascular bundles and the fruit (mericarp)
is semi-circular and lightly ribbed in cross section.
Non-glandular unicellular and bicellular cuneate trichomes
are sparsely distributed on the stem, leaves and petiole, but
very dense on the fruit. Secretory channels are present in all
organs: in the parenchyma of the root and stem cortex, stem
pith, in the phloem of root and vascular bundles of stem and
petiole, by the leaf vascular bundles and in the fruit pericarp.
Essential oil yields from different parts of P. tragium ranged
from 0.2-1.1% (v/w). The main compounds in the oils from
roots (in both stages, from both localities), as well as from
aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes
(trinorsesquiterpenes): pregeijerene (29.0-56.2%)
and gejerene (14.1-22.9%), whereas those from aerial parts
and fruits from village Izvor are β-bisabolene (19.1-57.2%)
and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate
(17.4-22.2%). Trinorsesquiterpenes, which were also
found to be characteristic constituents in some other Pimpinella
species oils, are the most dominant constituents in all
investigated oils, except in the oil of fruit from village Izvor.
In addition, phenylpropanoids of pseudoisoeugenol type are
present in all oils confi rming them as chemical markers of
Pimpinella species analysed so far.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition
VL  - 42
IS  - 1 supplement
SP  - 130
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5253
ER  - 
@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Kovačević, Nada and Drobac, Milica",
year = "2018",
abstract = "Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting
dry grasslands, limestone rocky areas and screes
of wide sense Mediterranean area, east European lowlands
and Caucasus. This study explores the anatomical structure,
content and composition of the essential oil of the vegetative
organs and fruit of this species. The plant material was collected
on two localities: the village Izvor (Bosilegrad, Serbia)
and Mt. Galičica (FYR Macedonia). The anatomical studies
were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope.
The essential oils obtained by hydrodistillation from roots
(collected during flowering and fruiting period), aerial parts
with inflorescences, and fruits, were analysed by GC-FID and
GC-MS. The anatomical analysis revealed a secondary structure
of root and primary structure of stem with closed collateral
vascular bundles. Leaves are isobilateral, amphistomatic;
petiole is with arched vascular bundles and the fruit (mericarp)
is semi-circular and lightly ribbed in cross section.
Non-glandular unicellular and bicellular cuneate trichomes
are sparsely distributed on the stem, leaves and petiole, but
very dense on the fruit. Secretory channels are present in all
organs: in the parenchyma of the root and stem cortex, stem
pith, in the phloem of root and vascular bundles of stem and
petiole, by the leaf vascular bundles and in the fruit pericarp.
Essential oil yields from different parts of P. tragium ranged
from 0.2-1.1% (v/w). The main compounds in the oils from
roots (in both stages, from both localities), as well as from
aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes
(trinorsesquiterpenes): pregeijerene (29.0-56.2%)
and gejerene (14.1-22.9%), whereas those from aerial parts
and fruits from village Izvor are β-bisabolene (19.1-57.2%)
and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate
(17.4-22.2%). Trinorsesquiterpenes, which were also
found to be characteristic constituents in some other Pimpinella
species oils, are the most dominant constituents in all
investigated oils, except in the oil of fruit from village Izvor.
In addition, phenylpropanoids of pseudoisoeugenol type are
present in all oils confi rming them as chemical markers of
Pimpinella species analysed so far.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition",
volume = "42",
number = "1 supplement",
pages = "130-130",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5253"
}
Slavkovska, V., Zlatković, B., Kovačević, N.,& Drobac, M.. (2018). Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 130-130.
https://hdl.handle.net/21.15107/rcub_farfar_5253
Slavkovska V, Zlatković B, Kovačević N, Drobac M. Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):130-130.
https://hdl.handle.net/21.15107/rcub_farfar_5253 .
Slavkovska, Violeta, Zlatković, Bojan, Kovačević, Nada, Drobac, Milica, "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):130-130,
https://hdl.handle.net/21.15107/rcub_farfar_5253 .