TY  - CONF
AU  - Spremo-Potparević, Biljana
AU  - Želježić, Davor
AU  - Bajić, Vladan
AU  - Popović, Petar
AU  - Živković, Lada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5471
AB  - Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.
PB  - Macedonian Academy of Sciences and Arts
C3  - 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
T1  - New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease
VL  - 26
IS  - Supplement
SP  - 88
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5471
ER  - 

@conference{
author = "Spremo-Potparević, Biljana and Želježić, Davor and Bajić, Vladan and Popović, Petar and Živković, Lada",
year = "2023",
abstract = "Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK",
title = "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease",
volume = "26",
number = "Supplement",
pages = "88-88",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5471"
}

Spremo-Potparević, B., Želježić, D., Bajić, V., Popović, P.,& Živković, L.. (2023). New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK
Macedonian Academy of Sciences and Arts., 26(Supplement), 88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471

Spremo-Potparević B, Želježić D, Bajić V, Popović P, Živković L. New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK. 2023;26(Supplement):88-88.
https://hdl.handle.net/21.15107/rcub_farfar_5471 .

Spremo-Potparević, Biljana, Želježić, Davor, Bajić, Vladan, Popović, Petar, Živković, Lada, "New approach in quantitative estimation  of x chromosome centromere instability  in Alzheimer disease" in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK, 26, no. Supplement (2023):88-88,
https://hdl.handle.net/21.15107/rcub_farfar_5471 .