TY  - JOUR
AU  - Lazić, Vesna
AU  - Vukoje, Ivana
AU  - Milicević, Bojana
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Bajić, Vladan
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan M.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3257
AB  - The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis
VL  - 84
IS  - 6
SP  - 539
EP  - 553
DO  - 10.2298/JSC181217017L
ER  - 

@article{
author = "Lazić, Vesna and Vukoje, Ivana and Milicević, Bojana and Potparević, Biljana and Živković, Lada and Topalović, Dijana and Bajić, Vladan and Sredojević, Dušan and Nedeljković, Jovan M.",
year = "2019",
abstract = "The genotoxic and antigenotoxic behavior of the interfacial charge transfer (ICT) complex between nano-sized TiO2 particles and caffeic acid (CA) was studied in in vitro experiments. The formation of the ICT complex is indicated by the appearance of absorption in visible-spectral range. The continual variations method indicated bridging coordination between the ligand, caffeic acid, and the surface Ti atoms, while the stability constant of the ICT complex was found to be 1.5x10(3) mol(-1) L. An agreement between the experimental data and the theoretical results, based on the density functional theory, was found. The ICT complex and its components did not display genotoxicity in the broad concentration range 0.4. 8.0 mg mL(-1) TiO2 at a mole ratio c(TiO2)/c(CA) = 8. On the other hand, post-treatment of damaged DNA by the ICT complex induced antigenotoxic effect at lower concentrations, but at higher concentrations, 5.125-10.250 mg mL(-1) ICT, the ICT complex did not show any beneficial effect on H2O2-induced DNA damaged cells. The experimental data were analyzed using the combinatorial method to determine the effect of component interaction on the genotoxic and antigenotoxic behavior of the ICT complex.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis",
volume = "84",
number = "6",
pages = "539-553",
doi = "10.2298/JSC181217017L"
}

Lazić, V., Vukoje, I., Milicević, B., Potparević, B., Živković, L., Topalović, D., Bajić, V., Sredojević, D.,& Nedeljković, J. M.. (2019). Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(6), 539-553.
https://doi.org/10.2298/JSC181217017L

Lazić V, Vukoje I, Milicević B, Potparević B, Živković L, Topalović D, Bajić V, Sredojević D, Nedeljković JM. Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis. in Journal of the Serbian Chemical Society. 2019;84(6):539-553.
doi:10.2298/JSC181217017L .

Lazić, Vesna, Vukoje, Ivana, Milicević, Bojana, Potparević, Biljana, Živković, Lada, Topalović, Dijana, Bajić, Vladan, Sredojević, Dušan, Nedeljković, Jovan M., "Efficiency of the interfacial charge transfer complex between TiO2 nanoparticles and caffeic acid against DNA damage in vitro: A combinatorial analysis" in Journal of the Serbian Chemical Society, 84, no. 6 (2019):539-553,
https://doi.org/10.2298/JSC181217017L . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Kotur-Stevuljević, Jelena
AU  - Isenović, Esma
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana
AU  - Lazić, Vesna
AU  - Ahrenkiel, S. Phillip
AU  - Nedeljković, Jovan M.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2968
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.
PB  - Elsevier Science BV, Amsterdam
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 

@article{
author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Pirković, Andrea and Kotur-Stevuljević, Jelena and Isenović, Esma and Sredojević, Dušan and Vukoje, Ivana and Lazić, Vesna and Ahrenkiel, S. Phillip and Nedeljković, Jovan M.",
year = "2017",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}

Bajić, V., Potparević, B., Živković, L., Pirković, A., Kotur-Stevuljević, J., Isenović, E., Sredojević, D., Vukoje, I., Lazić, V., Ahrenkiel, S. P.,& Nedeljković, J. M.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces
Elsevier Science BV, Amsterdam., 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032

Bajić V, Potparević B, Živković L, Pirković A, Kotur-Stevuljević J, Isenović E, Sredojević D, Vukoje I, Lazić V, Ahrenkiel SP, Nedeljković JM. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces B: Biointerfaces. 2017;155:323-331.
doi:10.1016/j.colsurfb.2017.04.032 .

Bajić, Vladan, Potparević, Biljana, Živković, Lada, Pirković, Andrea, Kotur-Stevuljević, Jelena, Isenović, Esma, Sredojević, Dušan, Vukoje, Ivana, Lazić, Vesna, Ahrenkiel, S. Phillip, Nedeljković, Jovan M., "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 . .

TY  - CONF
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Vukoje, Ivana
AU  - Kotur-Stevuljević, Jelena
AU  - Nedeljković, Jovan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5296
AB  - Ascorbic acid (AA) is a standard antioxidant and its radical scavenging properties also appear to be responsible for its antigenotoxic properties. Nanoparticle-mediated delivery of antioxidant compounds is intended to increase their bioavailability while maintaining their effectiveness.  Colloids consisting of the 45-Å TiO2 nanoparticles (NPs) with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color.  The aim of this study was to compare, for the first time, the antioxidant and antigenotoxic effects of AA attached to the surface of TiO2 NPs with free AA in a wide concentration range. For evaluation of antigenotoxic properties whole blood cells were first treated with 50 μM H2O2 to induce DNA damage, and then exposed to 3 different concentrations of free AA (1.3×10-2, 2.6×10-2, and 3.9×10-2 M) and the same concentrations of AA attached to TiO2 NPs (0.05, 0.1, and 0.15 M) for 30 min at 37 0C. The level of DNA damage was evaluated by comet assay method. For evaluation of antioxidant properties, total antioxidant capacity (TAC), total antioxidative status (TAS) and prooxidative-antioxidative balance (PAB) were determined in human serum pool during 2 and 24 h incubation at 37 °C, without and with terc-buthyl-hydroperoxide (TBH) as exogenously added oxidant. As expected, the results of DNA damage showed that the increase of AA concentration leads to a reduction of DNA damage. The similar concentration dependence was observed for surface-modified TiO2 NPs with AA. So, no significant differences between the antigenotoxic properties of free AA and AA attached to the TiO2 NPs were noticed, but only the highest concentrations showed significant effect in both experimental treatments. Regarding short-term oxidative balance in biological material (serum), during 2h, by measuring the TAC we have showed that the complex NP and AA, so as ascorbic acid showed a significant increase in TAC capacity, compared to native serum. This increase of antioxidative capacity couldn’t be abrogated even with a powerful oxidant, terc-buthylhydroperoxide presence (TBH). After 24h hour incubation the TAC level in both samples decreased towards the baseline level. About the TOS, which measures all oxidative components in plasma such as hydrogen-peroxide and lipid hydroperoxide, the complex of NP and AA versus AA alone showed inconsistent results. Prooxidative-antioxidative balance (PAB) measuring equilibrium between oxidants and antioxidants remains low, almost imponderable after 2h and after 24h serum incubation with the two substances. To summarize, we suggest that surface-modified TiO2 NPs with AA and/or similar compounds can be used to improve their bioavailability while maintaining its beneficial activities.
PB  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016
C3  - International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage
SP  - 51
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5296
ER  - 

@conference{
author = "Bajić, Vladan and Spremo-Potparević, Biljana and Živković, Lada and Čabarkapa, Andrea and Vukoje, Ivana and Kotur-Stevuljević, Jelena and Nedeljković, Jovan",
year = "2016",
abstract = "Ascorbic acid (AA) is a standard antioxidant and its radical scavenging properties also appear to be responsible for its antigenotoxic properties. Nanoparticle-mediated delivery of antioxidant compounds is intended to increase their bioavailability while maintaining their effectiveness.  Colloids consisting of the 45-Å TiO2 nanoparticles (NPs) with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color.  The aim of this study was to compare, for the first time, the antioxidant and antigenotoxic effects of AA attached to the surface of TiO2 NPs with free AA in a wide concentration range. For evaluation of antigenotoxic properties whole blood cells were first treated with 50 μM H2O2 to induce DNA damage, and then exposed to 3 different concentrations of free AA (1.3×10-2, 2.6×10-2, and 3.9×10-2 M) and the same concentrations of AA attached to TiO2 NPs (0.05, 0.1, and 0.15 M) for 30 min at 37 0C. The level of DNA damage was evaluated by comet assay method. For evaluation of antioxidant properties, total antioxidant capacity (TAC), total antioxidative status (TAS) and prooxidative-antioxidative balance (PAB) were determined in human serum pool during 2 and 24 h incubation at 37 °C, without and with terc-buthyl-hydroperoxide (TBH) as exogenously added oxidant. As expected, the results of DNA damage showed that the increase of AA concentration leads to a reduction of DNA damage. The similar concentration dependence was observed for surface-modified TiO2 NPs with AA. So, no significant differences between the antigenotoxic properties of free AA and AA attached to the TiO2 NPs were noticed, but only the highest concentrations showed significant effect in both experimental treatments. Regarding short-term oxidative balance in biological material (serum), during 2h, by measuring the TAC we have showed that the complex NP and AA, so as ascorbic acid showed a significant increase in TAC capacity, compared to native serum. This increase of antioxidative capacity couldn’t be abrogated even with a powerful oxidant, terc-buthylhydroperoxide presence (TBH). After 24h hour incubation the TAC level in both samples decreased towards the baseline level. About the TOS, which measures all oxidative components in plasma such as hydrogen-peroxide and lipid hydroperoxide, the complex of NP and AA versus AA alone showed inconsistent results. Prooxidative-antioxidative balance (PAB) measuring equilibrium between oxidants and antioxidants remains low, almost imponderable after 2h and after 24h serum incubation with the two substances. To summarize, we suggest that surface-modified TiO2 NPs with AA and/or similar compounds can be used to improve their bioavailability while maintaining its beneficial activities.",
publisher = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016",
journal = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage",
pages = "51-51",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5296"
}

Bajić, V., Spremo-Potparević, B., Živković, L., Čabarkapa, A., Vukoje, I., Kotur-Stevuljević, J.,& Nedeljković, J.. (2016). Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book
International Congress on Occupational & Environmental Toxicology - ICOETox 2016., 51-51.
https://hdl.handle.net/21.15107/rcub_farfar_5296

Bajić V, Spremo-Potparević B, Živković L, Čabarkapa A, Vukoje I, Kotur-Stevuljević J, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book. 2016;:51-51.
https://hdl.handle.net/21.15107/rcub_farfar_5296 .

Bajić, Vladan, Spremo-Potparević, Biljana, Živković, Lada, Čabarkapa, Andrea, Vukoje, Ivana, Kotur-Stevuljević, Jelena, Nedeljković, Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: antioxidant properties and efficiency against DNA damage" in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, June | Porto, Abstract book (2016):51-51,
https://hdl.handle.net/21.15107/rcub_farfar_5296 .