TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Čabarkapa-Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes-Hernández, Tamara
AU  - Zlatković-Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
PB  - Wiley
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.",
publisher = "Wiley",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry
Wiley., 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Perry, George
AU  - Živković, Lada
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2308
AB  - Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p lt 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p lt 0.001). In addition, a strong (R2=0.97, n=20) and significant (p lt 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Alzheimer Research
T1  - Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients
VL  - 12
IS  - 10
SP  - 990
EP  - 996
DO  - 10.2174/1567205012666151027124154
ER  - 

@article{
author = "Potparević, Biljana and Bajić, Vladan and Perry, George and Živković, Lada",
year = "2015",
abstract = "Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p lt 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p lt 0.001). In addition, a strong (R2=0.97, n=20) and significant (p lt 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Alzheimer Research",
title = "Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients",
volume = "12",
number = "10",
pages = "990-996",
doi = "10.2174/1567205012666151027124154"
}

Potparević, B., Bajić, V., Perry, G.,& Živković, L.. (2015). Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients. in Current Alzheimer Research
Bentham Science Publ Ltd, Sharjah., 12(10), 990-996.
https://doi.org/10.2174/1567205012666151027124154

Potparević B, Bajić V, Perry G, Živković L. Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients. in Current Alzheimer Research. 2015;12(10):990-996.
doi:10.2174/1567205012666151027124154 .

Potparević, Biljana, Bajić, Vladan, Perry, George, Živković, Lada, "Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients" in Current Alzheimer Research, 12, no. 10 (2015):990-996,
https://doi.org/10.2174/1567205012666151027124154 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Siedlak, Sandra L.
AU  - Perry, George
AU  - Plećaš-Solarović, Bosiljka
AU  - Milicević, Zorana
AU  - Bajić, Vladan
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1861
AB  - While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright
PB  - Karger, Basel
T2  - Neurodegenerative Diseases
T1  - DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division
VL  - 12
IS  - 3
SP  - 156
EP  - 163
DO  - 10.1159/000346114
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Siedlak, Sandra L. and Perry, George and Plećaš-Solarović, Bosiljka and Milicević, Zorana and Bajić, Vladan",
year = "2013",
abstract = "While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright",
publisher = "Karger, Basel",
journal = "Neurodegenerative Diseases",
title = "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division",
volume = "12",
number = "3",
pages = "156-163",
doi = "10.1159/000346114"
}

Živković, L., Potparević, B., Siedlak, S. L., Perry, G., Plećaš-Solarović, B., Milicević, Z.,& Bajić, V.. (2013). DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases
Karger, Basel., 12(3), 156-163.
https://doi.org/10.1159/000346114

Živković L, Potparević B, Siedlak SL, Perry G, Plećaš-Solarović B, Milicević Z, Bajić V. DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases. 2013;12(3):156-163.
doi:10.1159/000346114 .

Živković, Lada, Potparević, Biljana, Siedlak, Sandra L., Perry, George, Plećaš-Solarović, Bosiljka, Milicević, Zorana, Bajić, Vladan, "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division" in Neurodegenerative Diseases, 12, no. 3 (2013):156-163,
https://doi.org/10.1159/000346114 . .