TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Jevdović, Radosav
AU  - Živković, Jelena
AU  - Ražić, Slavica
AU  - Moravcević, Đorđe
AU  - Maksimović, Zoran
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3318
AB  - In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.
VL  - 130
SP  - 162
EP  - 169
DO  - 10.1016/j.indcrop.2018.12.055
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Jevdović, Radosav and Živković, Jelena and Ražić, Slavica and Moravcević, Đorđe and Maksimović, Zoran",
year = "2019",
abstract = "In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.",
volume = "130",
pages = "162-169",
doi = "10.1016/j.indcrop.2018.12.055"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Jevdović, R., Živković, J., Ražić, S., Moravcević, Đ.,& Maksimović, Z.. (2019). Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 130, 162-169.
https://doi.org/10.1016/j.indcrop.2018.12.055

Arsenijević J, Drobac M, Šoštarić I, Jevdović R, Živković J, Ražić S, Moravcević Đ, Maksimović Z. Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products. 2019;130:162-169.
doi:10.1016/j.indcrop.2018.12.055 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Jevdović, Radosav, Živković, Jelena, Ražić, Slavica, Moravcević, Đorđe, Maksimović, Zoran, "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All." in Industrial Crops and Products, 130 (2019):162-169,
https://doi.org/10.1016/j.indcrop.2018.12.055 . .

TY  - JOUR
AU  - Cebović, Tatjana
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Živković, Jelena
AU  - Šoštarić, Ivan
AU  - Maksimović, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3158
AB  - Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent
VL  - 55
IS  - 2
SP  - 560
EP  - 567
DO  - 10.1007/s13197-017-2965-1
ER  - 

@article{
author = "Cebović, Tatjana and Arsenijević, Jelena and Drobac, Milica and Živković, Jelena and Šoštarić, Ivan and Maksimović, Zoran",
year = "2018",
abstract = "Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent",
volume = "55",
number = "2",
pages = "560-567",
doi = "10.1007/s13197-017-2965-1"
}

Cebović, T., Arsenijević, J., Drobac, M., Živković, J., Šoštarić, I.,& Maksimović, Z.. (2018). Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(2), 560-567.
https://doi.org/10.1007/s13197-017-2965-1

Cebović T, Arsenijević J, Drobac M, Živković J, Šoštarić I, Maksimović Z. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore. 2018;55(2):560-567.
doi:10.1007/s13197-017-2965-1 .

Cebović, Tatjana, Arsenijević, Jelena, Drobac, Milica, Živković, Jelena, Šoštarić, Ivan, Maksimović, Zoran, "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent" in Journal of Food Science and Technology-Mysore, 55, no. 2 (2018):560-567,
https://doi.org/10.1007/s13197-017-2965-1 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Ražić, Slavica
AU  - Milenković, Marina
AU  - Couladis, Maria
AU  - Maksimović, Zoran
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2663
AB  - Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics
VL  - 89
SP  - 14
EP  - 20
EP  - 
DO  - 10.1016/j.indcrop.2016.04.046
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Ražić, Slavica and Milenković, Marina and Couladis, Maria and Maksimović, Zoran",
year = "2016",
abstract = "Hungarian thyme (Thymus pannonicus All., Lamiaceae) is an aromatic herb used as traditional remedy, a refreshing beverage and a food aromatizer. Herbal teas, i.e., infusions, of Hungarian thyme from eight localities in Serbia were analyzed regarding their polyphenolic and volatile composition, and tested for their antioxidant and antimicrobial activity. The total polyphenolics content, determined by the Folin-Ciocalteu method, ranged from 1122.25 to 1979.93 mg gallic acid/L. HPLC analysis revealed rosmarinic acid (367.42-1199.47 mg/L) and luteolin glucuronides as the main polyphenolics. The volatile fractions of the infusions, analyzed by static headspace extraction coupled with GC and GC-MS analyses, contained citral, 3-octanone, 1-octen-3-ol, linalool and 1,8-cineole as the dominant constituents. The antioxidant activity of the infusions was examined through the ferric reducing antioxidant power (FRAP) (68.09-124.58 mmol Fe2+/L) and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical tests (SC50 1.32-2.96 mu L/mL). The antimicrobial activity was tested by the broth microdilution method against standard strains of Gram(+) Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis and Enterococcus faecalis and Gram(-) bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, and one strain of yeast Candida albicans. The infusions inhibited microbial growth in the tested concentration range (31.25-500.00 mu L/mL) and the strongest activity was exhibited against the strain of C. albicans (MIC 31.25-62.50 mu L/mL). The antioxidant and antimicrobial properties of the infusions were, to some extent, in correlation with the composition and content of the polyphenolic compounds, whereas the volatiles noticeably influenced the exhibited antimicrobial activity.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics",
volume = "89",
pages = "14-20-",
doi = "10.1016/j.indcrop.2016.04.046"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Ražić, S., Milenković, M., Couladis, M.,& Maksimović, Z.. (2016). Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 89, 14-20.
https://doi.org/10.1016/j.indcrop.2016.04.046

Arsenijević J, Drobac M, Šoštarić I, Ražić S, Milenković M, Couladis M, Maksimović Z. Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics. in Industrial Crops and Products. 2016;89:14-20.
doi:10.1016/j.indcrop.2016.04.046 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Ražić, Slavica, Milenković, Marina, Couladis, Maria, Maksimović, Zoran, "Bioactivity of herbal tea of Hungarian thyme based on the composition of volatiles and polyphenolics" in Industrial Crops and Products, 89 (2016):14-20,
https://doi.org/10.1016/j.indcrop.2016.04.046 . .

TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Marković, Jelena
AU  - Šoštarić, Ivan
AU  - Ražić, Slavica
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1843
AB  - The volatile fraction of the leaves of Thymus pannonicus All. (Lamiaceae) was analyzed by headspace extraction followed by GC-FID and GC-MS analysis. The different headspace profiles were recognized, with citral and with monoterpene hydrocarbons as dominant compounds. In addition, the determination of Cr, Co, Ni, Mo, Cu, Zn, Mn, Fe, Mg, Ca, K and Na was conducted by spectroscopic techniques (FAAS, GFAAS and ICP-OES). In order to evaluate the relationship between volatile organic compounds and metals, a chemometrics approach was applied. The data obtained by analysis of the headspace and elemental content were subjected to correlation analysis, factor analysis, principal component analysis and cluster analysis. A number of significant correlations of metals with plant volatiles were found. Correlation of Zn with citral, Mn with oxygenated monoterpenes and Mg with beta-bourbonene, could be explained by involvement of metals in the biosynthesis of volatile organic compounds.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Plant Physiology and Biochemistry
T1  - A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples
VL  - 71
SP  - 298
EP  - 306
DO  - 10.1016/j.plaphy.2013.08.002
ER  - 

@article{
author = "Arsenijević, Jelena and Marković, Jelena and Šoštarić, Ivan and Ražić, Slavica",
year = "2013",
abstract = "The volatile fraction of the leaves of Thymus pannonicus All. (Lamiaceae) was analyzed by headspace extraction followed by GC-FID and GC-MS analysis. The different headspace profiles were recognized, with citral and with monoterpene hydrocarbons as dominant compounds. In addition, the determination of Cr, Co, Ni, Mo, Cu, Zn, Mn, Fe, Mg, Ca, K and Na was conducted by spectroscopic techniques (FAAS, GFAAS and ICP-OES). In order to evaluate the relationship between volatile organic compounds and metals, a chemometrics approach was applied. The data obtained by analysis of the headspace and elemental content were subjected to correlation analysis, factor analysis, principal component analysis and cluster analysis. A number of significant correlations of metals with plant volatiles were found. Correlation of Zn with citral, Mn with oxygenated monoterpenes and Mg with beta-bourbonene, could be explained by involvement of metals in the biosynthesis of volatile organic compounds.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Plant Physiology and Biochemistry",
title = "A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples",
volume = "71",
pages = "298-306",
doi = "10.1016/j.plaphy.2013.08.002"
}

Arsenijević, J., Marković, J., Šoštarić, I.,& Ražić, S.. (2013). A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 71, 298-306.
https://doi.org/10.1016/j.plaphy.2013.08.002

Arsenijević J, Marković J, Šoštarić I, Ražić S. A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples. in Plant Physiology and Biochemistry. 2013;71:298-306.
doi:10.1016/j.plaphy.2013.08.002 .

Arsenijević, Jelena, Marković, Jelena, Šoštarić, Ivan, Ražić, Slavica, "A chemometrics as a powerful tool in the elucidation of the role of metals in the biosynthesis of volatile organic compounds in Hungarian thyme samples" in Plant Physiology and Biochemistry, 71 (2013):298-306,
https://doi.org/10.1016/j.plaphy.2013.08.002 . .

TY  - JOUR
AU  - Šoštarić, Ivan
AU  - Arsenijević, Jelena
AU  - Acić, Svetlana
AU  - Dajić-Stevanović, Zora
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1669
AB  - Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia
VL  - 15
IS  - 2
SP  - 237
EP  - 243
DO  - 10.1080/0972060X.2012.10644041
ER  - 

@article{
author = "Šoštarić, Ivan and Arsenijević, Jelena and Acić, Svetlana and Dajić-Stevanović, Zora",
year = "2012",
abstract = "Three populations of Thymus pannonicus grown in Serbia (one from northern and two from east Serbia) were examined for chemical composition of their essential oil. Thymus pannonicus is used in traditional medicine and it is collected by local people in the studied regions, hence the interest in composition of its essential oils. Three major groups of essential oil components were identified, such as monoterpene hydrocarbons, oxygenated monoterpene hydrocarbons and sesquiterpene hydrocarbons. Each of investigated populations showed its own chemotype, including: geranial (citral), alpha-pinene and germacrene-D. To best of our knowledge, this sesquiterpene hydrocarbon was reported as a major component in Thymus bracteosus only. In these populations the phenolic compounds thymol and carvacrol, considered as most frequent constituents of essential oil in Thymus taxa, haven't been found in significant quantities.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia",
volume = "15",
number = "2",
pages = "237-243",
doi = "10.1080/0972060X.2012.10644041"
}

Šoštarić, I., Arsenijević, J., Acić, S.,& Dajić-Stevanović, Z.. (2012). Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 15(2), 237-243.
https://doi.org/10.1080/0972060X.2012.10644041

Šoštarić I, Arsenijević J, Acić S, Dajić-Stevanović Z. Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia. in Journal of Essential Oil Bearing Plants. 2012;15(2):237-243.
doi:10.1080/0972060X.2012.10644041 .

Šoštarić, Ivan, Arsenijević, Jelena, Acić, Svetlana, Dajić-Stevanović, Zora, "Essential Oil Polymorphism of Thymus pannonicus All. (Lamiaceae) in Serbia" in Journal of Essential Oil Bearing Plants, 15, no. 2 (2012):237-243,
https://doi.org/10.1080/0972060X.2012.10644041 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Šoštarić, Ivan
AU  - Marin, Petar
AU  - Stojanović, Danilo
AU  - Ristić, Mihailo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1143
AB  - In five indigenous populations of Thymus glabrescens Willd. collected in the region of Banat (Serbia), the variability on leaf morphological traits, leaf and stem anatomy, and composition of the essential oil was studied. The major component in the studied populations was either thymol or γ-terpinene. Distinct differentiation of populations with respect to chemical composition of essential oils might be related to spatial distribution of the studied populations. No correlations between morphology, anatomy, and essential oil yield and composition were determined. Both capitate and peltate glandular trichomes were found on calyces, whereas the latter were noticed on the abaxial and adaxial leaf surface.
AB  - U pet autohtonih populacija Thymus glabrescens Willd. sakupljenih u regionu Banata, Srbija, ispitivana je varijabilnost morfoloških karakteristika lista, anatomske građe lista i stabla i sastava etarskih ulja. Glavne komponente etarskih ulja proučavanih populacija bile su timol, γ-terpinen i p-cimen. Uočena je prostorna diferencijacija populacija u odnosu na sastav etarskih ulja. Nije ustanovljena korelacija između morfoloških i anatomskih karakteristika populacija i prinosa i sastava etarskih ulja. Prisustvo i izgled žlezdanih trihoma su utvrđeni posmatranjem nadzemnih delova na svetlosnom i skening elektronskom mikroskopu. Peltatne trihome su uočene na čašici, licu i naličju lista, dok su kapitatne nađene samo na čašicama.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition
T1  - Populaciona varijabilnost unutar vrste Thymus glabrescens Willd. iz Srbije - morfologija, anatomija i sastav etarskih ulja
VL  - 60
IS  - 3
SP  - 475
EP  - 483
DO  - 10.2298/ABS0803475D
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Šoštarić, Ivan and Marin, Petar and Stojanović, Danilo and Ristić, Mihailo",
year = "2008",
abstract = "In five indigenous populations of Thymus glabrescens Willd. collected in the region of Banat (Serbia), the variability on leaf morphological traits, leaf and stem anatomy, and composition of the essential oil was studied. The major component in the studied populations was either thymol or γ-terpinene. Distinct differentiation of populations with respect to chemical composition of essential oils might be related to spatial distribution of the studied populations. No correlations between morphology, anatomy, and essential oil yield and composition were determined. Both capitate and peltate glandular trichomes were found on calyces, whereas the latter were noticed on the abaxial and adaxial leaf surface., U pet autohtonih populacija Thymus glabrescens Willd. sakupljenih u regionu Banata, Srbija, ispitivana je varijabilnost morfoloških karakteristika lista, anatomske građe lista i stabla i sastava etarskih ulja. Glavne komponente etarskih ulja proučavanih populacija bile su timol, γ-terpinen i p-cimen. Uočena je prostorna diferencijacija populacija u odnosu na sastav etarskih ulja. Nije ustanovljena korelacija između morfoloških i anatomskih karakteristika populacija i prinosa i sastava etarskih ulja. Prisustvo i izgled žlezdanih trihoma su utvrđeni posmatranjem nadzemnih delova na svetlosnom i skening elektronskom mikroskopu. Peltatne trihome su uočene na čašici, licu i naličju lista, dok su kapitatne nađene samo na čašicama.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition, Populaciona varijabilnost unutar vrste Thymus glabrescens Willd. iz Srbije - morfologija, anatomija i sastav etarskih ulja",
volume = "60",
number = "3",
pages = "475-483",
doi = "10.2298/ABS0803475D"
}

Dajić-Stevanović, Z., Šoštarić, I., Marin, P., Stojanović, D.,& Ristić, M.. (2008). Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 475-483.
https://doi.org/10.2298/ABS0803475D

Dajić-Stevanović Z, Šoštarić I, Marin P, Stojanović D, Ristić M. Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition. in Archives of Biological Sciences. 2008;60(3):475-483.
doi:10.2298/ABS0803475D .

Dajić-Stevanović, Zora, Šoštarić, Ivan, Marin, Petar, Stojanović, Danilo, Ristić, Mihailo, "Population variability in Thymus glabrescens Willd. from Serbia: Morphology, anatomy and essential oil composition" in Archives of Biological Sciences, 60, no. 3 (2008):475-483,
https://doi.org/10.2298/ABS0803475D . .

TY  - JOUR
AU  - Maksimović, Zoran
AU  - Stojanović, Danilo
AU  - Šoštarić, Ivan
AU  - Dajić, Zora
AU  - Ristić, Mihailo
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1117
AB  - BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil
VL  - 88
IS  - 11
SP  - 2036
EP  - 2041
DO  - 10.1002/jsfa.3311
ER  - 

@article{
author = "Maksimović, Zoran and Stojanović, Danilo and Šoštarić, Ivan and Dajić, Zora and Ristić, Mihailo",
year = "2008",
abstract = "BACKGROUND: Knowledge about the chemical and therapeutic properties of Thymus glabrescens Willd. (Lamiaceae) is scarce and inconsistent. Therefore the main objectives of this study were to determine the yield and chemical composition of essential oils from wild-growing T. glabrescens populations, to assess their radical scavenging activity and to correlate the results with published data in order to deduce which components are responsible for the activity. RESULTS: The plant material yielded between 4.0 and 8.0 mL kg(-1) of essential oil. All samples contained considerable but variable concentrations of thymol (22.3-55.1%), depending on the source. Radical-scavenging activities of the oils were estimated by 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) assay against butylated hydroxytoluene (BHT) and thymol as positive controls. The observed activities (IC50 values ranged from 94 to 230 mu gmL(-1)) were strongly influenced by thymol concentration, as verified by rapid screening for DPPH radical-scavenging activity on thin layer chromatography (TLC) plates and regression analysis. CONCLUSION: These results represent the first report on the free radical-scavenging activity of T. glabrescens essential oil and one of the first comprehensive reports on its composition. Thymus glabrescens could be used in the food industry for seasoning purposes or for preserving processed foods from oxidative degradation.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil",
volume = "88",
number = "11",
pages = "2036-2041",
doi = "10.1002/jsfa.3311"
}

Maksimović, Z., Stojanović, D., Šoštarić, I., Dajić, Z.,& Ristić, M.. (2008). Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 88(11), 2036-2041.
https://doi.org/10.1002/jsfa.3311

Maksimović Z, Stojanović D, Šoštarić I, Dajić Z, Ristić M. Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil. in Journal of the Science of Food and Agriculture. 2008;88(11):2036-2041.
doi:10.1002/jsfa.3311 .

Maksimović, Zoran, Stojanović, Danilo, Šoštarić, Ivan, Dajić, Zora, Ristić, Mihailo, "Composition and radical-scavenging activity of Thymus glabrescens Willd. (Lamiaceae) essential oil" in Journal of the Science of Food and Agriculture, 88, no. 11 (2008):2036-2041,
https://doi.org/10.1002/jsfa.3311 . .