TY  - JOUR
AU  - Kozarević Cilović, Emina
AU  - Dautović, Esmeralda
AU  - Halilčević, Dalila
AU  - Softić, Adaleta
AU  - Srabović, Nahida
AU  - Šarić-Kundalić, Broza
AU  - Delić, Nina
AU  - Kolarević, Lamija
AU  - Mekić, Lejla
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4624
AB  - Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.
PB  - International Research Journal of Pure and Applied Chemistry
T2  - International Research Journal of Pure and Applied Chemistry
T1  - Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts
VL  - 23
IS  - 5
SP  - 23
EP  - 32
DO  - 10.9734/irjpac/2022/v23i5788
ER  - 

@article{
author = "Kozarević Cilović, Emina and Dautović, Esmeralda and Halilčević, Dalila and Softić, Adaleta and Srabović, Nahida and Šarić-Kundalić, Broza and Delić, Nina and Kolarević, Lamija and Mekić, Lejla and Ibišević, Merima and Horozić, Emir and Arsenijević, Jelena and Maksimović, Zoran",
year = "2022",
abstract = "Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.",
publisher = "International Research Journal of Pure and Applied Chemistry",
journal = "International Research Journal of Pure and Applied Chemistry",
title = "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts",
volume = "23",
number = "5",
pages = "23-32",
doi = "10.9734/irjpac/2022/v23i5788"
}

Kozarević Cilović, E., Dautović, E., Halilčević, D., Softić, A., Srabović, N., Šarić-Kundalić, B., Delić, N., Kolarević, L., Mekić, L., Ibišević, M., Horozić, E., Arsenijević, J.,& Maksimović, Z.. (2022). Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry
International Research Journal of Pure and Applied Chemistry., 23(5), 23-32.
https://doi.org/10.9734/irjpac/2022/v23i5788

Kozarević Cilović E, Dautović E, Halilčević D, Softić A, Srabović N, Šarić-Kundalić B, Delić N, Kolarević L, Mekić L, Ibišević M, Horozić E, Arsenijević J, Maksimović Z. Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry. 2022;23(5):23-32.
doi:10.9734/irjpac/2022/v23i5788 .

Kozarević Cilović, Emina, Dautović, Esmeralda, Halilčević, Dalila, Softić, Adaleta, Srabović, Nahida, Šarić-Kundalić, Broza, Delić, Nina, Kolarević, Lamija, Mekić, Lejla, Ibišević, Merima, Horozić, Emir, Arsenijević, Jelena, Maksimović, Zoran, "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts" in International Research Journal of Pure and Applied Chemistry, 23, no. 5 (2022):23-32,
https://doi.org/10.9734/irjpac/2022/v23i5788 . .

TY  - JOUR
AU  - Husejnović, Maida Sljivic
AU  - Bergant, Martina
AU  - Janković, Saša
AU  - Zizek, Suzana
AU  - Smajlović, Aida
AU  - Softić, Adaleta
AU  - Musić, Omer
AU  - Antonijević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3213
AB  - Soil contamination by heavy metals is a serious global environmental problem, especially for developing countries. A large number of industrial plants, which continually pollute the environment, characterize Tuzla Canton, Bosnia and Herzegovina. The aim of this study was to assess the level of soil pollution by heavy metals and to estimate cytotoxicity and genotoxicity of soil leachates from this area. Lead (Pb), cadmium (Cd) and mercury (Hg) were analyzed by ICP-AES and AAS. Soil contamination was assessed using contamination factor, degree of contamination, geoaccumulation index and pollution load index. To determine the connection of variables and understanding their origin in soils, principal component analysis (PCA) and cluster analysis (CA) were used. The results indicate that Cd and Hg originated from natural and anthropogenic activities, while Pb is of anthropogenic origin. For toxicity evaluation, CaCo-2 and HaCaT cells were used. PrestoBlue assay was used for cytotoxicity testing, and cH2A. X for genotoxicity evaluation. Concerning cytotoxicity, Cd and Hg had a positive correlation with cytotoxicity in HaCaT cells, but only Hg induced cytotoxicity in CaCo-2 cells. We also demonstrate that soil leachates contaminated by heavy metals can induce genotoxicity in both used cell lines. According to these results, combining bioassays with standard physicochemical analysis can be useful for evaluating environmental and health risks more accurately. These results are important for developing proper management strategies to decrease pollution. This is one of the first studies from this area and an important indication of soil quality in Southeast Europe.
PB  - Springer, Dordrecht
T2  - Environmental Geochemistry and Health
T1  - Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)
VL  - 40
IS  - 4
SP  - 1557
EP  - 1572
DO  - 10.1007/s10653-018-0071-6
ER  - 

@article{
author = "Husejnović, Maida Sljivic and Bergant, Martina and Janković, Saša and Zizek, Suzana and Smajlović, Aida and Softić, Adaleta and Musić, Omer and Antonijević, Biljana",
year = "2018",
abstract = "Soil contamination by heavy metals is a serious global environmental problem, especially for developing countries. A large number of industrial plants, which continually pollute the environment, characterize Tuzla Canton, Bosnia and Herzegovina. The aim of this study was to assess the level of soil pollution by heavy metals and to estimate cytotoxicity and genotoxicity of soil leachates from this area. Lead (Pb), cadmium (Cd) and mercury (Hg) were analyzed by ICP-AES and AAS. Soil contamination was assessed using contamination factor, degree of contamination, geoaccumulation index and pollution load index. To determine the connection of variables and understanding their origin in soils, principal component analysis (PCA) and cluster analysis (CA) were used. The results indicate that Cd and Hg originated from natural and anthropogenic activities, while Pb is of anthropogenic origin. For toxicity evaluation, CaCo-2 and HaCaT cells were used. PrestoBlue assay was used for cytotoxicity testing, and cH2A. X for genotoxicity evaluation. Concerning cytotoxicity, Cd and Hg had a positive correlation with cytotoxicity in HaCaT cells, but only Hg induced cytotoxicity in CaCo-2 cells. We also demonstrate that soil leachates contaminated by heavy metals can induce genotoxicity in both used cell lines. According to these results, combining bioassays with standard physicochemical analysis can be useful for evaluating environmental and health risks more accurately. These results are important for developing proper management strategies to decrease pollution. This is one of the first studies from this area and an important indication of soil quality in Southeast Europe.",
publisher = "Springer, Dordrecht",
journal = "Environmental Geochemistry and Health",
title = "Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)",
volume = "40",
number = "4",
pages = "1557-1572",
doi = "10.1007/s10653-018-0071-6"
}

Husejnović, M. S., Bergant, M., Janković, S., Zizek, S., Smajlović, A., Softić, A., Musić, O.,& Antonijević, B.. (2018). Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). in Environmental Geochemistry and Health
Springer, Dordrecht., 40(4), 1557-1572.
https://doi.org/10.1007/s10653-018-0071-6

Husejnović MS, Bergant M, Janković S, Zizek S, Smajlović A, Softić A, Musić O, Antonijević B. Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). in Environmental Geochemistry and Health. 2018;40(4):1557-1572.
doi:10.1007/s10653-018-0071-6 .

Husejnović, Maida Sljivic, Bergant, Martina, Janković, Saša, Zizek, Suzana, Smajlović, Aida, Softić, Adaleta, Musić, Omer, Antonijević, Biljana, "Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)" in Environmental Geochemistry and Health, 40, no. 4 (2018):1557-1572,
https://doi.org/10.1007/s10653-018-0071-6 . .