TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Šobajić, Slađana
AU  - Blagojević, Duško
AU  - Radusinović, Miodrag
AU  - El-Sohemy, Ahmed
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/927
AB  - Zinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.
PB  - Humana Press Inc, Totowa
T2  - Biological Trace Element Research
T1  - Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells
VL  - 116
IS  - 1
SP  - 61
EP  - 71
DO  - 10.1007/BF02685919
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Šobajić, Slađana and Blagojević, Duško and Radusinović, Miodrag and El-Sohemy, Ahmed",
year = "2007",
abstract = "Zinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.",
publisher = "Humana Press Inc, Totowa",
journal = "Biological Trace Element Research",
title = "Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells",
volume = "116",
number = "1",
pages = "61-71",
doi = "10.1007/BF02685919"
}

Cerović, A., Miletić, I., Šobajić, S., Blagojević, D., Radusinović, M.,& El-Sohemy, A.. (2007). Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells. in Biological Trace Element Research
Humana Press Inc, Totowa., 116(1), 61-71.
https://doi.org/10.1007/BF02685919

Cerović A, Miletić I, Šobajić S, Blagojević D, Radusinović M, El-Sohemy A. Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells. in Biological Trace Element Research. 2007;116(1):61-71.
doi:10.1007/BF02685919 .

Cerović, Aleksandra, Miletić, Ivanka, Šobajić, Slađana, Blagojević, Duško, Radusinović, Miodrag, El-Sohemy, Ahmed, "Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells" in Biological Trace Element Research, 116, no. 1 (2007):61-71,
https://doi.org/10.1007/BF02685919 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Šobajić, Slađana
AU  - Blagojević, Duško
AU  - Jones, David R.
AU  - Poznanić, Milica
AU  - Radusinović, Miodrag
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/707
AB  - The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin A on the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respectively). The following day, the animals were given sunflower oil containing 50 nmol vitamin A. The results showed that the concentration of zinc in blood plasma was similar in both groups. The amount of plasma chylomicrons was lower in the LZ group than in the SZ group (p  lt  0.001). The concentration of retinol in blood plasma was lower in the LZ group than in the SZ group (p  lt  0.01). However, the results demonstrated an increase in the blood plasma retinol concentration in the LZ group compared to the SZ group when calculated per milligram of plasma chylomicrons (p  lt  0.01). In plasma chylomicrons, fatty acids corresponding to 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0, and 20:4 were detected. The fatty acid distribution was similar in both groups. There was no major difference in the concentration of fatty acids in plasma chylomicrons between both experimental groups, except for 20:4 (a lower amount was found in the SZ group). Our results show that dietary zinc influences both the amount of chylomicrons in blood plasma and the concentrations of retinol and arachidonic acid in chylomicrons.
PB  - Humana Press Inc, Totowa
T2  - Biological Trace Element Research
T1  - Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons
VL  - 112
IS  - 2
SP  - 145
EP  - 158
DO  - 10.1385/BTER:112:2:145
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Šobajić, Slađana and Blagojević, Duško and Jones, David R. and Poznanić, Milica and Radusinović, Miodrag",
year = "2006",
abstract = "The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin A on the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respectively). The following day, the animals were given sunflower oil containing 50 nmol vitamin A. The results showed that the concentration of zinc in blood plasma was similar in both groups. The amount of plasma chylomicrons was lower in the LZ group than in the SZ group (p  lt  0.001). The concentration of retinol in blood plasma was lower in the LZ group than in the SZ group (p  lt  0.01). However, the results demonstrated an increase in the blood plasma retinol concentration in the LZ group compared to the SZ group when calculated per milligram of plasma chylomicrons (p  lt  0.01). In plasma chylomicrons, fatty acids corresponding to 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0, and 20:4 were detected. The fatty acid distribution was similar in both groups. There was no major difference in the concentration of fatty acids in plasma chylomicrons between both experimental groups, except for 20:4 (a lower amount was found in the SZ group). Our results show that dietary zinc influences both the amount of chylomicrons in blood plasma and the concentrations of retinol and arachidonic acid in chylomicrons.",
publisher = "Humana Press Inc, Totowa",
journal = "Biological Trace Element Research",
title = "Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons",
volume = "112",
number = "2",
pages = "145-158",
doi = "10.1385/BTER:112:2:145"
}

Cerović, A., Miletić, I., Šobajić, S., Blagojević, D., Jones, D. R., Poznanić, M.,& Radusinović, M.. (2006). Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons. in Biological Trace Element Research
Humana Press Inc, Totowa., 112(2), 145-158.
https://doi.org/10.1385/BTER:112:2:145

Cerović A, Miletić I, Šobajić S, Blagojević D, Jones DR, Poznanić M, Radusinović M. Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons. in Biological Trace Element Research. 2006;112(2):145-158.
doi:10.1385/BTER:112:2:145 .

Cerović, Aleksandra, Miletić, Ivanka, Šobajić, Slađana, Blagojević, Duško, Jones, David R., Poznanić, Milica, Radusinović, Miodrag, "Effect of dietary zinc on the levels and distribution of fatty acids and vitamin A in blood plasma chylomicrons" in Biological Trace Element Research, 112, no. 2 (2006):145-158,
https://doi.org/10.1385/BTER:112:2:145 . .