TY  - JOUR
AU  - Cirković, Ivana
AU  - Božić, Dragana
AU  - Draganić, Veselin
AU  - Lozo, Jelena
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Arsić, Biljana
AU  - Garalejić, Eliana
AU  - Đukić, Slobodanka
AU  - Stanković, Slavisa
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2583
AB  - Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates
VL  - 11
IS  - 12
DO  - 10.1371/journal.pone.0167995
ER  - 

@article{
author = "Cirković, Ivana and Božić, Dragana and Draganić, Veselin and Lozo, Jelena and Berić, Tanja and Kojić, Milan and Arsić, Biljana and Garalejić, Eliana and Đukić, Slobodanka and Stanković, Slavisa",
year = "2016",
abstract = "Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates",
volume = "11",
number = "12",
doi = "10.1371/journal.pone.0167995"
}

Cirković, I., Božić, D., Draganić, V., Lozo, J., Berić, T., Kojić, M., Arsić, B., Garalejić, E., Đukić, S.,& Stanković, S.. (2016). Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167995

Cirković I, Božić D, Draganić V, Lozo J, Berić T, Kojić M, Arsić B, Garalejić E, Đukić S, Stanković S. Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One. 2016;11(12).
doi:10.1371/journal.pone.0167995 .

Cirković, Ivana, Božić, Dragana, Draganić, Veselin, Lozo, Jelena, Berić, Tanja, Kojić, Milan, Arsić, Biljana, Garalejić, Eliana, Đukić, Slobodanka, Stanković, Slavisa, "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates" in PLoS One, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167995 . .