TY  - JOUR
AU  - Kuntić, Vesna
AU  - Filipović, Ivana
AU  - Vujić, Zorica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1450
AB  - Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 mu M prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.
PB  - MDPI, Basel
T2  - Molecules
T1  - Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays
VL  - 16
IS  - 2
SP  - 1378
EP  - 1388
DO  - 10.3390/molecules16021378
ER  - 

@article{
author = "Kuntić, Vesna and Filipović, Ivana and Vujić, Zorica",
year = "2011",
abstract = "Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 mu M prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays",
volume = "16",
number = "2",
pages = "1378-1388",
doi = "10.3390/molecules16021378"
}

Kuntić, V., Filipović, I.,& Vujić, Z.. (2011). Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. in Molecules
MDPI, Basel., 16(2), 1378-1388.
https://doi.org/10.3390/molecules16021378

Kuntić V, Filipović I, Vujić Z. Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. in Molecules. 2011;16(2):1378-1388.
doi:10.3390/molecules16021378 .

Kuntić, Vesna, Filipović, Ivana, Vujić, Zorica, "Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays" in Molecules, 16, no. 2 (2011):1378-1388,
https://doi.org/10.3390/molecules16021378 . .

TY  - JOUR
AU  - Holclajtner-Antunović, Ivanka
AU  - Kuntić, Vesna
AU  - Juranić, Zorica
AU  - Filipović, Ivana M.
AU  - Mioč, Ubavka B.
AU  - Stanojković, Tatjana
AU  - Žižak, Željko
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/556
AB  - The antitumour action of three polyoxometallate compounds of Keggin's type: 12-molibdophosphoric acid (MoPA), 12-tungstophosphoric acid (WPA) and Mg salt of WPA (MgHWPA) was studied in vitro. For human cervix carcinoma (HeLa) cells survival, as well as for nonstimulated and stimulated peripheral blood mononuclear cells (PBMC), MTT test was applied and IC50 values of POMs were determinated. Index selectivity for WPA and MgHWPA are 1.9 and 1.8, calculated for nonstimulated, as well as 2.5 and 2.0, calculated for stimulated PBMS. Combination of studied POMs do not contribute to their lower IC50 values. Apoptosis detection implies mild cytotoxic effect of WPA and more cytostatical effect of MgHWPA. Combination of each of the studied POMs with caffeine decreases HeLa survival in dose dependent way. None of the studied POMs in the used concentrations (up to 100 mmol/L) damages blood cells and/or decreases their number.
AB  - Ispitivano je in vitro antitumorsko dejstvo tri jedinjenja iz grupe polioksometalata (POM-a) Keginovog tipa: 12-fosformolibdenska kiselina (MoPA), 12-fosforvolframova kiselina (WPA) i Mg so 12-fosforvolframove kiseline (MgHWPA). Primenom MTT testa na ćelije karcinoma cerviksa (HeLa ćelije), kao i na nestimulisane i stimulisane mononuklearne ćelije iz periferne krvi (PBMC), određene su IC50 vrednosti ispitivanih POM-a. Indeksi selektivnosti za WPA i MgHWPA su 1,9 i 1,8 računati za nestimulisane, odnosno 2,5 i 2,0 za stimulisane PBMC. Kombinacijom ispitivanih POMa ne postiže se smanjenje njihovih IC50 vrednosti. Detekcijom apoptoze utvrđeno je da WPA pokazuje blagi citotoksični efekat, dok MgHWPA ima blagi citotostatski efekat. U kombinaciji sa kofeinom, POM jedinjenja još više smanjuju preživljavanje HeLa ćelija. U ispitivanim koncentracijama (do 100 mmol/L), nijedno od POM jedinjenja ne utiče na broj i izgled krvnih ćelija pune krvi zdravih davalaca.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Jugoslovenska medicinska biohemija
T1  - Study of some polyoxometallates of Keggin's type as potential antitumour agents
T1  - Ispitivanje nekih polioksalometalata Keginovog tipa kao potencijalnih antitumorskih agenasa
VL  - 23
IS  - 1
SP  - 25
EP  - 30
DO  - 10.2298/JMH0401025H
ER  - 

@article{
author = "Holclajtner-Antunović, Ivanka and Kuntić, Vesna and Juranić, Zorica and Filipović, Ivana M. and Mioč, Ubavka B. and Stanojković, Tatjana and Žižak, Željko",
year = "2004",
abstract = "The antitumour action of three polyoxometallate compounds of Keggin's type: 12-molibdophosphoric acid (MoPA), 12-tungstophosphoric acid (WPA) and Mg salt of WPA (MgHWPA) was studied in vitro. For human cervix carcinoma (HeLa) cells survival, as well as for nonstimulated and stimulated peripheral blood mononuclear cells (PBMC), MTT test was applied and IC50 values of POMs were determinated. Index selectivity for WPA and MgHWPA are 1.9 and 1.8, calculated for nonstimulated, as well as 2.5 and 2.0, calculated for stimulated PBMS. Combination of studied POMs do not contribute to their lower IC50 values. Apoptosis detection implies mild cytotoxic effect of WPA and more cytostatical effect of MgHWPA. Combination of each of the studied POMs with caffeine decreases HeLa survival in dose dependent way. None of the studied POMs in the used concentrations (up to 100 mmol/L) damages blood cells and/or decreases their number., Ispitivano je in vitro antitumorsko dejstvo tri jedinjenja iz grupe polioksometalata (POM-a) Keginovog tipa: 12-fosformolibdenska kiselina (MoPA), 12-fosforvolframova kiselina (WPA) i Mg so 12-fosforvolframove kiseline (MgHWPA). Primenom MTT testa na ćelije karcinoma cerviksa (HeLa ćelije), kao i na nestimulisane i stimulisane mononuklearne ćelije iz periferne krvi (PBMC), određene su IC50 vrednosti ispitivanih POM-a. Indeksi selektivnosti za WPA i MgHWPA su 1,9 i 1,8 računati za nestimulisane, odnosno 2,5 i 2,0 za stimulisane PBMC. Kombinacijom ispitivanih POMa ne postiže se smanjenje njihovih IC50 vrednosti. Detekcijom apoptoze utvrđeno je da WPA pokazuje blagi citotoksični efekat, dok MgHWPA ima blagi citotostatski efekat. U kombinaciji sa kofeinom, POM jedinjenja još više smanjuju preživljavanje HeLa ćelija. U ispitivanim koncentracijama (do 100 mmol/L), nijedno od POM jedinjenja ne utiče na broj i izgled krvnih ćelija pune krvi zdravih davalaca.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska medicinska biohemija",
title = "Study of some polyoxometallates of Keggin's type as potential antitumour agents, Ispitivanje nekih polioksalometalata Keginovog tipa kao potencijalnih antitumorskih agenasa",
volume = "23",
number = "1",
pages = "25-30",
doi = "10.2298/JMH0401025H"
}

Holclajtner-Antunović, I., Kuntić, V., Juranić, Z., Filipović, I. M., Mioč, U. B., Stanojković, T.,& Žižak, Ž.. (2004). Study of some polyoxometallates of Keggin's type as potential antitumour agents. in Jugoslovenska medicinska biohemija
Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd., 23(1), 25-30.
https://doi.org/10.2298/JMH0401025H

Holclajtner-Antunović I, Kuntić V, Juranić Z, Filipović IM, Mioč UB, Stanojković T, Žižak Ž. Study of some polyoxometallates of Keggin's type as potential antitumour agents. in Jugoslovenska medicinska biohemija. 2004;23(1):25-30.
doi:10.2298/JMH0401025H .

Holclajtner-Antunović, Ivanka, Kuntić, Vesna, Juranić, Zorica, Filipović, Ivana M., Mioč, Ubavka B., Stanojković, Tatjana, Žižak, Željko, "Study of some polyoxometallates of Keggin's type as potential antitumour agents" in Jugoslovenska medicinska biohemija, 23, no. 1 (2004):25-30,
https://doi.org/10.2298/JMH0401025H . .

TY  - CONF
AU  - Filipović, Ivana M.
AU  - Kuntić, Vesna
AU  - Mioč, Ubavka B.
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/364
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Investigated of anticoagulation effects of Mg salt of phosphotungstic acid
T1  - Ispitivanje antikoagulantnog dejstva Mg soli fosforvolframove kiseline
VL  - 52
IS  - 4
SP  - 706
EP  - 707
UR  - https://hdl.handle.net/21.15107/rcub_farfar_364
ER  - 

@conference{
author = "Filipović, Ivana M. and Kuntić, Vesna and Mioč, Ubavka B.",
year = "2002",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Investigated of anticoagulation effects of Mg salt of phosphotungstic acid, Ispitivanje antikoagulantnog dejstva Mg soli fosforvolframove kiseline",
volume = "52",
number = "4",
pages = "706-707",
url = "https://hdl.handle.net/21.15107/rcub_farfar_364"
}

Filipović, I. M., Kuntić, V.,& Mioč, U. B.. (2002). Investigated of anticoagulation effects of Mg salt of phosphotungstic acid. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 52(4), 706-707.
https://hdl.handle.net/21.15107/rcub_farfar_364

Filipović IM, Kuntić V, Mioč UB. Investigated of anticoagulation effects of Mg salt of phosphotungstic acid. in Arhiv za farmaciju. 2002;52(4):706-707.
https://hdl.handle.net/21.15107/rcub_farfar_364 .

Filipović, Ivana M., Kuntić, Vesna, Mioč, Ubavka B., "Investigated of anticoagulation effects of Mg salt of phosphotungstic acid" in Arhiv za farmaciju, 52, no. 4 (2002):706-707,
https://hdl.handle.net/21.15107/rcub_farfar_364 .