TY  - JOUR
AU  - Miseljić, Branislava
AU  - Popović, Gordana
AU  - Agbaba, Danica
AU  - Marković, Slavko
AU  - Simonovska, Breda
AU  - Vovk, Irena
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1077
AB  - A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.
PB  - AOAC Int, Gaithersburg
T2  - Journal of AOAC International
T1  - Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals
VL  - 91
IS  - 2
SP  - 332
EP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1077
ER  - 

@article{
author = "Miseljić, Branislava and Popović, Gordana and Agbaba, Danica and Marković, Slavko and Simonovska, Breda and Vovk, Irena",
year = "2008",
abstract = "A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals",
volume = "91",
number = "2",
pages = "332-338",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1077"
}

Miseljić, B., Popović, G., Agbaba, D., Marković, S., Simonovska, B.,& Vovk, I.. (2008). Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals. in Journal of AOAC International
AOAC Int, Gaithersburg., 91(2), 332-338.
https://hdl.handle.net/21.15107/rcub_farfar_1077

Miseljić B, Popović G, Agbaba D, Marković S, Simonovska B, Vovk I. Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals. in Journal of AOAC International. 2008;91(2):332-338.
https://hdl.handle.net/21.15107/rcub_farfar_1077 .

Miseljić, Branislava, Popović, Gordana, Agbaba, Danica, Marković, Slavko, Simonovska, Breda, Vovk, Irena, "Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals" in Journal of AOAC International, 91, no. 2 (2008):332-338,
https://hdl.handle.net/21.15107/rcub_farfar_1077 .