TY  - JOUR
AU  - Martić, Radava
AU  - Kotur-Stevuljević, Jelena
AU  - Malenović, Anđelija
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4200
AB  - Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
VL  - 17
IS  - 7
DO  - 10.1177/1934578X221112811
ER  - 

@article{
author = "Martić, Radava and Kotur-Stevuljević, Jelena and Malenović, Anđelija and Ušjak, Ljuboš and Petrović, Silvana and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2022",
abstract = "Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment",
volume = "17",
number = "7",
doi = "10.1177/1934578X221112811"
}

Martić, R., Kotur-Stevuljević, J., Malenović, A., Ušjak, L., Petrović, S., Čalija, B., Milić, J.,& Krajišnik, D.. (2022). Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications
SAGE Publications Inc.., 17(7).
https://doi.org/10.1177/1934578X221112811

Martić R, Kotur-Stevuljević J, Malenović A, Ušjak L, Petrović S, Čalija B, Milić J, Krajišnik D. Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications. 2022;17(7).
doi:10.1177/1934578X221112811 .

Martić, Radava, Kotur-Stevuljević, Jelena, Malenović, Anđelija, Ušjak, Ljuboš, Petrović, Silvana, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment" in Natural Product Communications, 17, no. 7 (2022),
https://doi.org/10.1177/1934578X221112811 . .

TY  - JOUR
AU  - Martić, Radava
AU  - Krajišnik, Danina
AU  - Milić, Jela
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3095
AB  - It is known that long-term exposure to ultraviolet (UV) radiation causes skin redness, solar erythema or burns, affects the structure of dermal connective tissue, increases the production of free radicals and the expression of matrix metalloproteinases, and can lead to the development of skin cancer. The World Health Organization recommends protective measures from the adverse effects of UV radiation, including the topical application of sunscreen products. One of the strategies for improving the quality and effectiveness of sunscreen products is introduction of new, more efficient and safer active molecules that absorb, reflect or disperse UV photons, as well as the introduction of substances that can prevent, neutralize or even repair damage caused by UV radiation. Significant potential for skin protection against harmful UV radiation is recognized in plant origin substances, which primarily exhibit an antioxidant effect, and additionally possess other photoprotective properties, which makes them interesting for further investigation. This paper presents an overview of the physicochemical properties of plant-based antioxidants, which are important for the formulation of the final cosmetic product and an overview of the potential effects of these substances in skin protection against UV radiation.
AB  - Poznato je da produženo izlaganje kože ultravioletnom (UV) zračenju dovodi do pojave crvenila, solarnog eritema ili opekotina, da utiče na strukturu vezivnog tkiva kože, proizvodnju slobodnih radikala, stimuliše proizvodnju matriks metaloproteinaza i da može dovesti do razvoja kancera kože. Svetska zdravstvena organizacija predlaže niz preventivnih mera za zaštitu kože od negativnih efekata UV zračenja, među kojima je i primena na kožu proizvoda za zaštitu od UV zračenja. Jedna od strategija za poboljšanje kvaliteta i efikasnosti proizvoda za zaštitu od UV zračenja je korišćenje novih, efikasnijih i bezbednijih aktivnih molekula koji apsorbuju, reflektuju ili rasipaju UV fotone, kao i uvođenje supstanci/materijala koji mogu da spreče, neutrališu i čak poprave oštećenja kože nastala delovanjem UV zračenja. Značajan potencijal za zaštitu kože od UV zračenja prepoznat je u supstancama/sastojcima biljnog porekla, koje primarno ispoljavaju antioksidantni efekat, a dodatno, poseduju i druge fotozaštitne osobine, što ih čini interesantnim za detaljnija istraživanja. U ovom radu je dat pregled fizičkohemijskih karakteristika antioksidanasa biljnog porekla, koje su značajne za formulaciju finalnog kozmetičkog proizvoda i potencijalnih efekata ovih sirovina/materijala u zaštiti kože od UV zračenja.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Antioxidants of plant origin in cosmetic products: Physicochemical properties and photoprotective potential
T1  - Antioksidansi biljnog porekla u kozmetičkim proizvodima - fizičkohemijske osobine i fotoprotektivni potencijal
VL  - 68
IS  - 1
SP  - 1
EP  - 33
DO  - 10.5937/arhFarm1801001M
ER  - 

@article{
author = "Martić, Radava and Krajišnik, Danina and Milić, Jela",
year = "2018",
abstract = "It is known that long-term exposure to ultraviolet (UV) radiation causes skin redness, solar erythema or burns, affects the structure of dermal connective tissue, increases the production of free radicals and the expression of matrix metalloproteinases, and can lead to the development of skin cancer. The World Health Organization recommends protective measures from the adverse effects of UV radiation, including the topical application of sunscreen products. One of the strategies for improving the quality and effectiveness of sunscreen products is introduction of new, more efficient and safer active molecules that absorb, reflect or disperse UV photons, as well as the introduction of substances that can prevent, neutralize or even repair damage caused by UV radiation. Significant potential for skin protection against harmful UV radiation is recognized in plant origin substances, which primarily exhibit an antioxidant effect, and additionally possess other photoprotective properties, which makes them interesting for further investigation. This paper presents an overview of the physicochemical properties of plant-based antioxidants, which are important for the formulation of the final cosmetic product and an overview of the potential effects of these substances in skin protection against UV radiation., Poznato je da produženo izlaganje kože ultravioletnom (UV) zračenju dovodi do pojave crvenila, solarnog eritema ili opekotina, da utiče na strukturu vezivnog tkiva kože, proizvodnju slobodnih radikala, stimuliše proizvodnju matriks metaloproteinaza i da može dovesti do razvoja kancera kože. Svetska zdravstvena organizacija predlaže niz preventivnih mera za zaštitu kože od negativnih efekata UV zračenja, među kojima je i primena na kožu proizvoda za zaštitu od UV zračenja. Jedna od strategija za poboljšanje kvaliteta i efikasnosti proizvoda za zaštitu od UV zračenja je korišćenje novih, efikasnijih i bezbednijih aktivnih molekula koji apsorbuju, reflektuju ili rasipaju UV fotone, kao i uvođenje supstanci/materijala koji mogu da spreče, neutrališu i čak poprave oštećenja kože nastala delovanjem UV zračenja. Značajan potencijal za zaštitu kože od UV zračenja prepoznat je u supstancama/sastojcima biljnog porekla, koje primarno ispoljavaju antioksidantni efekat, a dodatno, poseduju i druge fotozaštitne osobine, što ih čini interesantnim za detaljnija istraživanja. U ovom radu je dat pregled fizičkohemijskih karakteristika antioksidanasa biljnog porekla, koje su značajne za formulaciju finalnog kozmetičkog proizvoda i potencijalnih efekata ovih sirovina/materijala u zaštiti kože od UV zračenja.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Antioxidants of plant origin in cosmetic products: Physicochemical properties and photoprotective potential, Antioksidansi biljnog porekla u kozmetičkim proizvodima - fizičkohemijske osobine i fotoprotektivni potencijal",
volume = "68",
number = "1",
pages = "1-33",
doi = "10.5937/arhFarm1801001M"
}

Martić, R., Krajišnik, D.,& Milić, J.. (2018). Antioxidants of plant origin in cosmetic products: Physicochemical properties and photoprotective potential. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 68(1), 1-33.
https://doi.org/10.5937/arhFarm1801001M

Martić R, Krajišnik D, Milić J. Antioxidants of plant origin in cosmetic products: Physicochemical properties and photoprotective potential. in Arhiv za farmaciju. 2018;68(1):1-33.
doi:10.5937/arhFarm1801001M .

Martić, Radava, Krajišnik, Danina, Milić, Jela, "Antioxidants of plant origin in cosmetic products: Physicochemical properties and photoprotective potential" in Arhiv za farmaciju, 68, no. 1 (2018):1-33,
https://doi.org/10.5937/arhFarm1801001M . .