@conference{
author = "Erceg, Sanja and Tomašević, Ratko and Ninić, Ana and Pavlović, Aleksandar and Guzonjić, Azra and Vujčić, Sanja and Mamić, Milica and Mitrović, Bojan and Gluvić, Zoran and Kotur-Stevuljević, Jelena",
year = "2022",
abstract = "Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease,
present in up to 30% of the adult population worldwide (1). Triglycerides accumulation in
hepatocytes (steatosis) represents the root cause of NAFLD and is associated with oxidative
stress, which could further lead to fibrosis and cell death of hepatocytes (2). The aim of this
research was to identify redox status markers for predicting the risk of developing steatosis.
158 participants were included. Steatosis was confirmed by ultrasound in 101 subjects,
while the remaining 57 were in the control group. The following markers of redox status
were determined in serum and plasma samples of all subjects: superoxide dismutase 1
(SOD1), paraoxonase (PON1), malondialdehyde (MDA) and superoxide anion (O2.-). For this
purpose, spectrophotometric methods and enzyme immunosorbent assays were used. SOD1
was statistically significantly higher (P<0.001), while O2 .- was significantly lower in the
patient group (P<0.001). SOD1 was significantly negatively correlated with O2.- (ρ= -0.494,
P<0.001) and MDA (ρ= -0.242, P=0.002). Univariate binary logistic regression analysis
showed a positive association between SOD1 and the presence of steatosis (OR=1.018, 95%
CI 1.005-1.031; P=0.005), as well as a negative association between O2 .- and the presence of
steatosis (OR=0.959, 95% CI 0.941-0.978; P<0.001). Multivariate analysis singled out SOD1
(OR=1.024, 95% CI 1.006-1.041; P=0.007) and O2.- (OR=0.965, 95% CI 0.942-0.989;
P=0.004) as independent predictors for the presence of steatosis in our subjects. The redox
status parameters, SOD1 and O2, respectively, showed a positive and negative prediction of
the presence of statosis in our subjects., Nealkoholna masna bolest jetre (eng. nonalcoholic fatty liver disease, NAFLD) je
najčešće hronično oboljenje jetre, prisutno i u do 30% adultne populacije širom sveta (1).
Značajnu ulogu u nastanku NAFLD ima akumulacija triglicerida u hepatocitima – steatoza,
koja je povezana sa oksidativnim stresom, a koji dalje vodi fibrozi i ćelijskoj smrti hepatocita
(2). Cilj ovog istraživanja bio je identifikacija markera redoks statusa za predviđanje rizika za
nastanak steatoze. Studija je obuhvatila 158 ispitanika iz Kliničko bolničkog centra Zemun.
Steatoza je potvrđena ultrazvukom kod 101 ispitanika, dok je preostalih 57 činilo kontrolnu
grupu. U uzorcima seruma i plazme svih ispitanika određeni su sledeći markeri redoks
statusa: superoksid dismutaza 1 (SOD1), paraoksonaza (PON1), malondialdehid (MDA) i
superoksidni anjon (O2.-). U tu svrhu korišćene su spektrofotometrijske metode i enzimski
imunosorbentni testovi. SOD1 je bila statistički značajno viša (P<0,001), dok O 2.- značajno
niži u grupi pacijenata (P<0,001), dok se PON1 i MDA nisu značajno razlikovali između
grupa. SOD1 je bila u značajnoj negativnoj korelaciji sa O 2.- (ρ=-0,494, P<0,001) i MDA (ρ= -
0,242, P=0,002). Univarijatna binarna logistička regresiona analiza je pokazala pozitivnu
asocijaciju između SOD1 i prisustva steatoze (OR=1,018, 95% CI 1,005-1,031; P=0,005), kao i
negativnu asocijaciju između O 2.- i prisustva steatoze (OR=0,959, 95% CI 0,941-0,978;
P<0,001). Multivarijantna analiza je izdvojila SOD1 (OR=1,024, 95% CI 1,006-1,041;
P=0,007) i O2.- (OR=0,965, 95% CI 0,942-0,989; P=0,004) kao nezavisne prediktore za
prisustvo steatoze kod naših ispitanika. Parametri redoks statusa, SOD1 i O 2.- redom, su
pokazali pozitivnu, odnosno negativnu predikciju prisustva statoze kod naših ispitanika.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Markers of redox status in patients with nonalcoholic fatty liver disease, Markeri redoks statusa kod pacijenata sa nealkoholnom masnom bolešću jetre",
volume = "72",
number = "4 suplement",
pages = "S233-S234",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4511"
}
