TY  - JOUR
AU  - Vučković, Nemanja
AU  - Prlainović, Nevena
AU  - Glođović, Nikola
AU  - Čalija, Bojan
AU  - Milosavljević, Nedeljko
AU  - Kalagasidis Krušić, Melina
AU  - Milašinović, Nikola
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5504
AB  - Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.
T2  - Journal of the Iranian Chemical Society
T1  - Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system
VL  - 21
IS  - 3
SP  - 781
EP  - 792
DO  - 10.1007/s13738-023-02960-1
ER  - 

@article{
author = "Vučković, Nemanja and Prlainović, Nevena and Glođović, Nikola and Čalija, Bojan and Milosavljević, Nedeljko and Kalagasidis Krušić, Melina and Milašinović, Nikola",
year = "2024",
abstract = "Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.",
journal = "Journal of the Iranian Chemical Society",
title = "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system",
volume = "21",
number = "3",
pages = "781-792",
doi = "10.1007/s13738-023-02960-1"
}

Vučković, N., Prlainović, N., Glođović, N., Čalija, B., Milosavljević, N., Kalagasidis Krušić, M.,& Milašinović, N.. (2024). Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society, 21(3), 781-792.
https://doi.org/10.1007/s13738-023-02960-1

Vučković N, Prlainović N, Glođović N, Čalija B, Milosavljević N, Kalagasidis Krušić M, Milašinović N. Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society. 2024;21(3):781-792.
doi:10.1007/s13738-023-02960-1 .

Vučković, Nemanja, Prlainović, Nevena, Glođović, Nikola, Čalija, Bojan, Milosavljević, Nedeljko, Kalagasidis Krušić, Melina, Milašinović, Nikola, "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system" in Journal of the Iranian Chemical Society, 21, no. 3 (2024):781-792,
https://doi.org/10.1007/s13738-023-02960-1 . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Čalija, Bojan
AU  - Dobričić, Vladimir
AU  - Jurišić Dukovsk, Bisera
AU  - Lovrić, Jasmina
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4566
AB  - To improve ocular bioavailability of eye drops, viscosity-increasing polymers with improved
functional properties have been used (1,2). The aim of this study was formulation and evaluation of
viscous eye drops using vehicles containing hydroxypropyl guar gum (HPG) (0.25% w/v), sodium
hyaluronate (SH) (0.4% w/v), and their combination (HPG-SH) as carriers for olopatadine (0.1% w/v).
Physicochemical properties (appearance, clarity, pH, osmolality, viscosity) of the prepared
formulations were tested. The drug permeability was estimated in vitro using HCE-T cell-based models
and the parallel artificial membrane permeability assay (PAMPA). MTT cytotoxicity assay was
performed at the end of the permeability study. Physicochemical stability of the formulated eye drops
was tested during 12 months. An accelerated stability study was performed using the heating/cooling
cycles test. The clarity, pH and osmolality of all formulations were in acceptable range for ophthalmic
preparations. Formulation HPG-SH showed significantly higher viscosity (73.1 mPa·s) than
formulations with single polymer (7.4 mPa·s for HPG i.e 3.7 mPa·s for SH) pointing to synergistic effect
of polymers. The addition of polymers led to a decrease in transcorneal permeability and lower
permeability coefficients with prolonged residence of drug at the ocular surface. The results of MTT
assay demonstrated that the tested formulations were biocompatible and well tolerated. Formulated
eye drops showed satisfactory physicochemical stability under all storage conditions.
Olopatadine was successfully formulated in biocompatible viscous ophthalmic solutions. The
combination of viscosity enhancer HPG with mucoadhesive polymer SH has a potential to improve
precorneal residence time and therapeutic effect of olopatadine.
AB  - Polimeri poboljšanih funkcionalnih karakteristika koji povećavaju viskozitet koriste se u
kapima za oči kako bi se povećala okularna raspoloživost (1,2). Cilj ove studije bio je formulacija i
ispitivanje viskoznih kapi za oči korišćenjem vehikuluma koji sadrže hidroksipropil guar gumu (HPG)
(0,25% v/v), natrijum hijaluronat (SH) (0,4% v/v) i njihovu kombinaciju (HPG-SH) kao nosača za
olopatadin (0,1% v/v). Ispitivane su fizičkohemijske osobine (izgled, bistrina, pH, osmolalnost,
viskozitet) pripremljenih formulacija. Permeabilnost leka je procenjena in vitro korišćenjem modela
zasnovanih na HCE-T ćelijama i testa permeabilnosti na paralelnim veštačkim membranama (PAMPA).
MTT citotoksični test sproveden je na kraju studije permeabilnosti. Fizičkohemijska stabilnost
formulisanih kapi za oči ispitivana je tokom 12 meseci. Sprovedena je ubrzana studija stabilnosti
korišćenjem cikličnog testa grejanja/hlađenja. Bistrina, pH i osmolalnost svih formulacija bili su u
prihvatljivom opsegu za oftalmološke preparate. Formulacija HPG-SH je pokazala značajno viši
viskozitet (73,1 mPa·s) od formulacija sa pojedinačnim polimerom (7,4 mPa·s za HPG, tj. 3,7 mPa·s za
SH) ukazujući na sinergistički efekat polimera. Dodatak polimera je doveo do smanjenja
transkornealne permeabilnosti i nižih koeficijenata permeabilnosti uz produženo zadržavanje leka na
površini oka. Rezultati MTT testa su pokazali da su testirane formulacije biokompatibilne i dobro
podnošljive. Formulisane kapi za oči imale su zadovoljavajuću fizičkohemijsku stabilnost pri svim
uslovima čuvanja. Uspešno su formulisani biokompatibilni viskozni rastvori za oftalmološku primenu
sa olopatadinom. Kombinacija HPG kao sredstva za povećanje viskoziteta sa mukoadhezivnim
polimerom SH ima potencijal da poboljša prekornealno vreme zadržavanja i terapijski efekat
olopatadina.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine
T1  - Procena uticaja hidroksipropil guar gume i hijaluronske kiseline na fizičkohemijske i funkcionalne karakteristike oftalmoloških vehikuluma za olopatadin
VL  - 72
IS  - 4 suplement
SP  - S388
EP  - S389
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4566
ER  - 

@conference{
author = "Račić, Anđelka and Čalija, Bojan and Dobričić, Vladimir and Jurišić Dukovsk, Bisera and Lovrić, Jasmina and Krajišnik, Danina",
year = "2022",
abstract = "To improve ocular bioavailability of eye drops, viscosity-increasing polymers with improved
functional properties have been used (1,2). The aim of this study was formulation and evaluation of
viscous eye drops using vehicles containing hydroxypropyl guar gum (HPG) (0.25% w/v), sodium
hyaluronate (SH) (0.4% w/v), and their combination (HPG-SH) as carriers for olopatadine (0.1% w/v).
Physicochemical properties (appearance, clarity, pH, osmolality, viscosity) of the prepared
formulations were tested. The drug permeability was estimated in vitro using HCE-T cell-based models
and the parallel artificial membrane permeability assay (PAMPA). MTT cytotoxicity assay was
performed at the end of the permeability study. Physicochemical stability of the formulated eye drops
was tested during 12 months. An accelerated stability study was performed using the heating/cooling
cycles test. The clarity, pH and osmolality of all formulations were in acceptable range for ophthalmic
preparations. Formulation HPG-SH showed significantly higher viscosity (73.1 mPa·s) than
formulations with single polymer (7.4 mPa·s for HPG i.e 3.7 mPa·s for SH) pointing to synergistic effect
of polymers. The addition of polymers led to a decrease in transcorneal permeability and lower
permeability coefficients with prolonged residence of drug at the ocular surface. The results of MTT
assay demonstrated that the tested formulations were biocompatible and well tolerated. Formulated
eye drops showed satisfactory physicochemical stability under all storage conditions.
Olopatadine was successfully formulated in biocompatible viscous ophthalmic solutions. The
combination of viscosity enhancer HPG with mucoadhesive polymer SH has a potential to improve
precorneal residence time and therapeutic effect of olopatadine., Polimeri poboljšanih funkcionalnih karakteristika koji povećavaju viskozitet koriste se u
kapima za oči kako bi se povećala okularna raspoloživost (1,2). Cilj ove studije bio je formulacija i
ispitivanje viskoznih kapi za oči korišćenjem vehikuluma koji sadrže hidroksipropil guar gumu (HPG)
(0,25% v/v), natrijum hijaluronat (SH) (0,4% v/v) i njihovu kombinaciju (HPG-SH) kao nosača za
olopatadin (0,1% v/v). Ispitivane su fizičkohemijske osobine (izgled, bistrina, pH, osmolalnost,
viskozitet) pripremljenih formulacija. Permeabilnost leka je procenjena in vitro korišćenjem modela
zasnovanih na HCE-T ćelijama i testa permeabilnosti na paralelnim veštačkim membranama (PAMPA).
MTT citotoksični test sproveden je na kraju studije permeabilnosti. Fizičkohemijska stabilnost
formulisanih kapi za oči ispitivana je tokom 12 meseci. Sprovedena je ubrzana studija stabilnosti
korišćenjem cikličnog testa grejanja/hlađenja. Bistrina, pH i osmolalnost svih formulacija bili su u
prihvatljivom opsegu za oftalmološke preparate. Formulacija HPG-SH je pokazala značajno viši
viskozitet (73,1 mPa·s) od formulacija sa pojedinačnim polimerom (7,4 mPa·s za HPG, tj. 3,7 mPa·s za
SH) ukazujući na sinergistički efekat polimera. Dodatak polimera je doveo do smanjenja
transkornealne permeabilnosti i nižih koeficijenata permeabilnosti uz produženo zadržavanje leka na
površini oka. Rezultati MTT testa su pokazali da su testirane formulacije biokompatibilne i dobro
podnošljive. Formulisane kapi za oči imale su zadovoljavajuću fizičkohemijsku stabilnost pri svim
uslovima čuvanja. Uspešno su formulisani biokompatibilni viskozni rastvori za oftalmološku primenu
sa olopatadinom. Kombinacija HPG kao sredstva za povećanje viskoziteta sa mukoadhezivnim
polimerom SH ima potencijal da poboljša prekornealno vreme zadržavanja i terapijski efekat
olopatadina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine, Procena uticaja hidroksipropil guar gume i hijaluronske kiseline na fizičkohemijske i funkcionalne karakteristike oftalmoloških vehikuluma za olopatadin",
volume = "72",
number = "4 suplement",
pages = "S388-S389",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4566"
}

Račić, A., Čalija, B., Dobričić, V., Jurišić Dukovsk, B., Lovrić, J.,& Krajišnik, D.. (2022). Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S388-S389.
https://hdl.handle.net/21.15107/rcub_farfar_4566

Račić A, Čalija B, Dobričić V, Jurišić Dukovsk B, Lovrić J, Krajišnik D. Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine. in Arhiv za farmaciju. 2022;72(4 suplement):S388-S389.
https://hdl.handle.net/21.15107/rcub_farfar_4566 .

Račić, Anđelka, Čalija, Bojan, Dobričić, Vladimir, Jurišić Dukovsk, Bisera, Lovrić, Jasmina, Krajišnik, Danina, "Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S388-S389,
https://hdl.handle.net/21.15107/rcub_farfar_4566 .

TY  - CONF
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Čalija, Bojan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4565
AB  - Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications.
AB  - Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites
T1  - Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance
VL  - 72
IS  - 4 suplement
SP  - S384
EP  - S385
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4565
ER  - 

@conference{
author = "Jauković, Valentina and Krajišnik, Danina and Čalija, Bojan",
year = "2022",
abstract = "Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications., Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites, Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance",
volume = "72",
number = "4 suplement",
pages = "S384-S385",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4565"
}

Jauković, V., Krajišnik, D.,& Čalija, B.. (2022). Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565

Jauković V, Krajišnik D, Čalija B. Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju. 2022;72(4 suplement):S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

Jauković, Valentina, Krajišnik, Danina, Čalija, Bojan, "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S384-S385,
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

TY  - JOUR
AU  - Martić, Radava
AU  - Kotur-Stevuljević, Jelena
AU  - Malenović, Anđelija
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4200
AB  - Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
VL  - 17
IS  - 7
DO  - 10.1177/1934578X221112811
ER  - 

@article{
author = "Martić, Radava and Kotur-Stevuljević, Jelena and Malenović, Anđelija and Ušjak, Ljuboš and Petrović, Silvana and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2022",
abstract = "Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment",
volume = "17",
number = "7",
doi = "10.1177/1934578X221112811"
}

Martić, R., Kotur-Stevuljević, J., Malenović, A., Ušjak, L., Petrović, S., Čalija, B., Milić, J.,& Krajišnik, D.. (2022). Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications
SAGE Publications Inc.., 17(7).
https://doi.org/10.1177/1934578X221112811

Martić R, Kotur-Stevuljević J, Malenović A, Ušjak L, Petrović S, Čalija B, Milić J, Krajišnik D. Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications. 2022;17(7).
doi:10.1177/1934578X221112811 .

Martić, Radava, Kotur-Stevuljević, Jelena, Malenović, Anđelija, Ušjak, Ljuboš, Petrović, Silvana, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment" in Natural Product Communications, 17, no. 7 (2022),
https://doi.org/10.1177/1934578X221112811 . .

TY  - JOUR
AU  - Račić, Andjelka
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Jurišić Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Micov, Ana
AU  - Vuković, Milja
AU  - Stepanović-Petrović, Radica
AU  - Krajišnik, Danina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3938
AB  - The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.
PB  - Elsevier B.V.
T2  - European Journal of Pharmaceutical Sciences
T1  - Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches
VL  - 166
DO  - 10.1016/j.ejps.2021.105906
ER  - 

@article{
author = "Račić, Andjelka and Čalija, Bojan and Milić, Jela and Jurišić Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Micov, Ana and Vuković, Milja and Stepanović-Petrović, Radica and Krajišnik, Danina",
year = "2021",
abstract = "The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.",
publisher = "Elsevier B.V.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches",
volume = "166",
doi = "10.1016/j.ejps.2021.105906"
}

Račić, A., Čalija, B., Milić, J., Jurišić Dukovski, B., Lovrić, J., Dobričić, V., Micov, A., Vuković, M., Stepanović-Petrović, R.,& Krajišnik, D.. (2021). Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches. in European Journal of Pharmaceutical Sciences
Elsevier B.V.., 166.
https://doi.org/10.1016/j.ejps.2021.105906

Račić A, Čalija B, Milić J, Jurišić Dukovski B, Lovrić J, Dobričić V, Micov A, Vuković M, Stepanović-Petrović R, Krajišnik D. Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches. in European Journal of Pharmaceutical Sciences. 2021;166.
doi:10.1016/j.ejps.2021.105906 .

Račić, Andjelka, Čalija, Bojan, Milić, Jela, Jurišić Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Micov, Ana, Vuković, Milja, Stepanović-Petrović, Radica, Krajišnik, Danina, "Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches" in European Journal of Pharmaceutical Sciences, 166 (2021),
https://doi.org/10.1016/j.ejps.2021.105906 . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Jurišić Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5481
AB  - Olopatadine is a selective H1-receptore antagonist that
inhibits release of histamine and proinflammatory
mediators [1]. The poor ocular bioavailability, as the main
limitation of efficient ocular therapy, could be overcome by
increasing residence time and enhancing corneal
penetration. ...
PB  - International Association for Pharmaceutical Technology, Mainz, Germany
C3  - 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting
T1  - Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5481
ER  - 

@conference{
author = "Račić, Anđelka and Jurišić Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2021",
abstract = "Olopatadine is a selective H1-receptore antagonist that
inhibits release of histamine and proinflammatory
mediators [1]. The poor ocular bioavailability, as the main
limitation of efficient ocular therapy, could be overcome by
increasing residence time and enhancing corneal
penetration. ...",
publisher = "International Association for Pharmaceutical Technology, Mainz, Germany",
journal = "12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting",
title = "Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5481"
}

Račić, A., Jurišić Dukovski, B., Lovrić, J., Dobričić, V., Čalija, B., Milić, J.,& Krajišnik, D.. (2021). Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model. in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting
International Association for Pharmaceutical Technology, Mainz, Germany..
https://hdl.handle.net/21.15107/rcub_farfar_5481

Račić A, Jurišić Dukovski B, Lovrić J, Dobričić V, Čalija B, Milić J, Krajišnik D. Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model. in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5481 .

Račić, Anđelka, Jurišić Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Permeability and biocompatibility evaluation of olopatadine hydrochloride viscous ophthalmic solutions using in vitro 3D corneal model" in 12th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, 11-14 May 2021, Vienna, Austria, Virtual meeting (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5481 .

TY  - JOUR
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Daković, Aleksandra
AU  - Damjanović, Ana
AU  - Krstić, Jugoslav
AU  - Stojanović, Jovica
AU  - Čalija, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3796
AB  - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
VL  - 123
DO  - 10.1016/j.msec.2021.112029
ER  - 

@article{
author = "Jauković, Valentina and Krajišnik, Danina and Daković, Aleksandra and Damjanović, Ana and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan",
year = "2021",
abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release",
volume = "123",
doi = "10.1016/j.msec.2021.112029"
}

Jauković, V., Krajišnik, D., Daković, A., Damjanović, A., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C
Elsevier Ltd., 123.
https://doi.org/10.1016/j.msec.2021.112029

Jauković V, Krajišnik D, Daković A, Damjanović A, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123.
doi:10.1016/j.msec.2021.112029 .

Jauković, Valentina, Krajišnik, Danina, Daković, Aleksandra, Damjanović, Ana, Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021),
https://doi.org/10.1016/j.msec.2021.112029 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Medarević, Đorđe
AU  - Čalija, Bojan
AU  - Dobričić, Vladimir
AU  - Rmandić, Milena
AU  - Barudžija, Tanja
AU  - Malenović, Anđelija
AU  - Đekić, Ljiljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3759
AB  - The effect of the entrapment procedure of a poorly water soluble drug (ibuprofen) on physicochemical and drug release performances of chitosan/xanthan polyelectrolyte complexes (PECs) was investigated to achieve controlled drug release as the ultimate goal. The formation of PECs for two drug entrapment procedures (before or after the mixing of polymers) at pH 4.6 and 5.6 and three chitosan-to-xanthan mass ratios (1:1, 1:2 and 1:3) was observed by continuous decrease in conductivity during the PECs formation and increased apparent viscosity and hysteresis values. The most extensive crosslinking was observed with ibuprofen added before the PECs formation at pH 4.6 and chitosan-to-xanthan mass ratio 1:1. The PECs prepared at polymers' mass ratios 1:2 and 1:3 had higher yield and drug entrapment efficiency. DSC and FT-IR analysis confirmed ibuprofen entrapment in PECs and the partial disruption of its crystallinity. All ibuprofen release profiles were similar, with 60–70% of drug released after 12 h, mainly by diffusion, but erosion and polymer chain relaxation were also included. Potentially optimal can be considered the PEC prepared at pH 4.6, ibuprofen entrapped before the mixing of polymers at chitosan-to-xanthan mass ratio 1:2, which provided controlled drug release by zero-order kinetics, high yield, and drug entrapment efficiency.
PB  - Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Effect of ibuprofen entrapment procedure on physicochemical and controlled drug release performances of chitosan/xanthan gum polyelectrolyte complexes
VL  - 167
SP  - 547
EP  - 558
DO  - 10.1016/j.ijbiomac.2020.11.201
ER  - 

@article{
author = "Ćirić, Ana and Medarević, Đorđe and Čalija, Bojan and Dobričić, Vladimir and Rmandić, Milena and Barudžija, Tanja and Malenović, Anđelija and Đekić, Ljiljana",
year = "2021",
abstract = "The effect of the entrapment procedure of a poorly water soluble drug (ibuprofen) on physicochemical and drug release performances of chitosan/xanthan polyelectrolyte complexes (PECs) was investigated to achieve controlled drug release as the ultimate goal. The formation of PECs for two drug entrapment procedures (before or after the mixing of polymers) at pH 4.6 and 5.6 and three chitosan-to-xanthan mass ratios (1:1, 1:2 and 1:3) was observed by continuous decrease in conductivity during the PECs formation and increased apparent viscosity and hysteresis values. The most extensive crosslinking was observed with ibuprofen added before the PECs formation at pH 4.6 and chitosan-to-xanthan mass ratio 1:1. The PECs prepared at polymers' mass ratios 1:2 and 1:3 had higher yield and drug entrapment efficiency. DSC and FT-IR analysis confirmed ibuprofen entrapment in PECs and the partial disruption of its crystallinity. All ibuprofen release profiles were similar, with 60–70% of drug released after 12 h, mainly by diffusion, but erosion and polymer chain relaxation were also included. Potentially optimal can be considered the PEC prepared at pH 4.6, ibuprofen entrapped before the mixing of polymers at chitosan-to-xanthan mass ratio 1:2, which provided controlled drug release by zero-order kinetics, high yield, and drug entrapment efficiency.",
publisher = "Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Effect of ibuprofen entrapment procedure on physicochemical and controlled drug release performances of chitosan/xanthan gum polyelectrolyte complexes",
volume = "167",
pages = "547-558",
doi = "10.1016/j.ijbiomac.2020.11.201"
}

Ćirić, A., Medarević, Đ., Čalija, B., Dobričić, V., Rmandić, M., Barudžija, T., Malenović, A.,& Đekić, L.. (2021). Effect of ibuprofen entrapment procedure on physicochemical and controlled drug release performances of chitosan/xanthan gum polyelectrolyte complexes. in International Journal of Biological Macromolecules
Elsevier B.V.., 167, 547-558.
https://doi.org/10.1016/j.ijbiomac.2020.11.201

Ćirić A, Medarević Đ, Čalija B, Dobričić V, Rmandić M, Barudžija T, Malenović A, Đekić L. Effect of ibuprofen entrapment procedure on physicochemical and controlled drug release performances of chitosan/xanthan gum polyelectrolyte complexes. in International Journal of Biological Macromolecules. 2021;167:547-558.
doi:10.1016/j.ijbiomac.2020.11.201 .

Ćirić, Ana, Medarević, Đorđe, Čalija, Bojan, Dobričić, Vladimir, Rmandić, Milena, Barudžija, Tanja, Malenović, Anđelija, Đekić, Ljiljana, "Effect of ibuprofen entrapment procedure on physicochemical and controlled drug release performances of chitosan/xanthan gum polyelectrolyte complexes" in International Journal of Biological Macromolecules, 167 (2021):547-558,
https://doi.org/10.1016/j.ijbiomac.2020.11.201 . .

TY  - JOUR
AU  - Mitrović, Jelena
AU  - Divović, Branka
AU  - Knutson, Daniel E.
AU  - Đoković, Jelena
AU  - Vulić, Predrag
AU  - Ranđelović, Danijela
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Cook, James M.
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3640
AB  - DK-I-56–1 (7‑methoxy‑2-(4‑methoxy‑d3-phenyl)-2,5-dihydro-3H-pyrazolo[4,3-c]quinolin-3-one), a recently developed deuterated pyrazoloquinolinone, has been recognized as a lead candidate for treatment of various neuropsychiatric disorders. During preclinical investigation of poorly water-soluble compounds such as DK-I-56–1, the application of nanotechnology could be advantageous due to improved safety and possibly increased bioavailability of nanosized formulation. DK-I-56–1 nanosuspensions stabilized by polysorbate 80, alone or in combination with poloxamers 188 i.e. 407 or d-α-tocopheryl polyethylene glycol 1000 succinate, were prepared using a small-scale media milling device. With particle size 208.7–250.6 nm and polydispersity index <0.250, selected nanodiseprsions were stable for three weeks. Pharmacokinetic and biodistribution studies following intraperitoneal administration of three types of formulation in mice indicated high plasma DK-I-56–1 levels after solution (10,228.6 ± 1037.2 ngh/ml) and nanosuspension (6770.4 ± 770.7 ngh/ml) but not suspension administration (966.0 ± 58.1 ngh/ml). However, distribution of DK-I-56–1 after solution was heavily influenced by its composition, and brain availability of nanosuspension was superior to that of solution formulation. In spontaneous locomotor activity test, the expected hyperlocomotor effect was observed after nanosuspension administration, without compromising impact of the vehicle/excipients used. Therefore, nanonization of drug compound assembled with proper selection of stabilizers may seemingly contribute further thorough testing of DK-I-56–1 preclinical efficacy.
PB  - Elsevier B.V.
T2  - European Journal of Pharmaceutical Sciences
T1  - Nanocrystal dispersion of DK-I-56–1, a poorly soluble pyrazoloquinolinone positive modulator of α6 GABAA receptors: Formulation approach toward improved in vivo performance
VL  - 152
DO  - 10.1016/j.ejps.2020.105432
ER  - 

@article{
author = "Mitrović, Jelena and Divović, Branka and Knutson, Daniel E. and Đoković, Jelena and Vulić, Predrag and Ranđelović, Danijela and Dobričić, Vladimir and Čalija, Bojan and Cook, James M. and Savić, Miroslav and Savić, Snežana",
year = "2020",
abstract = "DK-I-56–1 (7‑methoxy‑2-(4‑methoxy‑d3-phenyl)-2,5-dihydro-3H-pyrazolo[4,3-c]quinolin-3-one), a recently developed deuterated pyrazoloquinolinone, has been recognized as a lead candidate for treatment of various neuropsychiatric disorders. During preclinical investigation of poorly water-soluble compounds such as DK-I-56–1, the application of nanotechnology could be advantageous due to improved safety and possibly increased bioavailability of nanosized formulation. DK-I-56–1 nanosuspensions stabilized by polysorbate 80, alone or in combination with poloxamers 188 i.e. 407 or d-α-tocopheryl polyethylene glycol 1000 succinate, were prepared using a small-scale media milling device. With particle size 208.7–250.6 nm and polydispersity index <0.250, selected nanodiseprsions were stable for three weeks. Pharmacokinetic and biodistribution studies following intraperitoneal administration of three types of formulation in mice indicated high plasma DK-I-56–1 levels after solution (10,228.6 ± 1037.2 ngh/ml) and nanosuspension (6770.4 ± 770.7 ngh/ml) but not suspension administration (966.0 ± 58.1 ngh/ml). However, distribution of DK-I-56–1 after solution was heavily influenced by its composition, and brain availability of nanosuspension was superior to that of solution formulation. In spontaneous locomotor activity test, the expected hyperlocomotor effect was observed after nanosuspension administration, without compromising impact of the vehicle/excipients used. Therefore, nanonization of drug compound assembled with proper selection of stabilizers may seemingly contribute further thorough testing of DK-I-56–1 preclinical efficacy.",
publisher = "Elsevier B.V.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Nanocrystal dispersion of DK-I-56–1, a poorly soluble pyrazoloquinolinone positive modulator of α6 GABAA receptors: Formulation approach toward improved in vivo performance",
volume = "152",
doi = "10.1016/j.ejps.2020.105432"
}

Mitrović, J., Divović, B., Knutson, D. E., Đoković, J., Vulić, P., Ranđelović, D., Dobričić, V., Čalija, B., Cook, J. M., Savić, M.,& Savić, S.. (2020). Nanocrystal dispersion of DK-I-56–1, a poorly soluble pyrazoloquinolinone positive modulator of α6 GABAA receptors: Formulation approach toward improved in vivo performance. in European Journal of Pharmaceutical Sciences
Elsevier B.V.., 152.
https://doi.org/10.1016/j.ejps.2020.105432

Mitrović J, Divović B, Knutson DE, Đoković J, Vulić P, Ranđelović D, Dobričić V, Čalija B, Cook JM, Savić M, Savić S. Nanocrystal dispersion of DK-I-56–1, a poorly soluble pyrazoloquinolinone positive modulator of α6 GABAA receptors: Formulation approach toward improved in vivo performance. in European Journal of Pharmaceutical Sciences. 2020;152.
doi:10.1016/j.ejps.2020.105432 .

Mitrović, Jelena, Divović, Branka, Knutson, Daniel E., Đoković, Jelena, Vulić, Predrag, Ranđelović, Danijela, Dobričić, Vladimir, Čalija, Bojan, Cook, James M., Savić, Miroslav, Savić, Snežana, "Nanocrystal dispersion of DK-I-56–1, a poorly soluble pyrazoloquinolinone positive modulator of α6 GABAA receptors: Formulation approach toward improved in vivo performance" in European Journal of Pharmaceutical Sciences, 152 (2020),
https://doi.org/10.1016/j.ejps.2020.105432 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Krajišnik, Danina
AU  - Čalija, Bojan
AU  - Đekić, Ljiljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3654
AB  - The formulation of biocompatible drug carriers based on cationic biopolymer chitosan and natural or synthetic polymers represents an important research interest. Therefore, this review aims to perceive their potential in drug delivery. The most investigated chitosan-based polymer blends are polyelectrolyte complexes (PECs) obtained by establishing ionic interactions with biocompatible   polyanions   as   alginates,   pectin,   xanthan   gum,   carrageenan, carboxymethylcellulose, and collagen. Depending on the preparation conditions, PECs could be prepared  in  versatile  forms  including  membranes/films,  hydrogel  beads,  nanoparticles,  and microparticles, to achieve controlled (e.g., extended, delayed, colon-specific and pH-dependent) drug delivery. PECs can encapsulate hydrophilic and lipophilic drug substances with different molecular  weights.  Drug  encapsulation  allows  the  preservation  of  their  structure,  activity, improvement in absorption efficiency, reduction in adverse effects and long-term stability in vitroand in  vivo. The biocompatible structures as non-covalent chitosan-based complexes could be formed also by establishing hydrogen bonds, for example with poly(vinyl alcohol). The swelling of these complexes is not pH-dependent and encapsulated drug substances are often released by already known types of diffusion. Moreover, grafted chitosan derivatives (e.g., carboxymethyl chitosan, trimethyl chitosan, acrylated chitosan) are synthesized to improve water solubility at a wide pH range and enhance the encapsulation capacity of promising PEC-based drug carriers.
AB  - Formulacija biokompatibilnih nosača lekovitih supstanci na bazi katjonskog biopolimera hitozana i prirodnih ili sintetskih polimera predstavlja značajan istraživački interes. Stoga je cilj ovog rada sagledati njihovu potencijalnu primenu kao nosača lekovitih supstanci. Najistraženije blende polimera na bazi hitozana su polielektrolitni kompleksi (PEK) dobijeni uspostavljanjem jonskih interakcija sa biokompatibilnim polianjonima, npr. alginatom, pektinom, ksantan gumom, karagenanom, karboksimetilcelulozom i kolagenom. U zavisnosti od uslova pripreme, mogu se formulisati PEK u vidu membrana/filmova, hidrogelnih perli, nanočestica, mikročestica ili drugih tipova nosača, sa ciljem postizanja kontrolisanog (npr. produženog, odloženog, kolon-specifičnog i pH-zavisnog) oslobađanja lekovitih supstanci. PEK su pogodni za inkapsulaciju hidrofilnih ili lipofilnih lekovitih supstanci različitih molekulskih masa. Inkapsulacija obezbeđuje očuvanje njihove strukture, aktivnosti, poboljšanje apsorpcije, smanjenje štetnih efekata i dugoročnu stabilnost in vitro i in vivo. Biokompatibilne strukture nalik kompleksima na bazi hitozana mogu se formirati i uspostavljanjem vodoničnih veza, kao što je slučaj sa polivinil alkoholom. Njihovo bubrenje ne zavisi od pH. Inkapsulirane lekovite supstance se najčešće oslobađaju prema nekom od već poznatih tipova difuzije. Dodatno, različiti derivati hitozana (npr. karboksimetilhitozan, trimetilhitozan, akril derivati hitozana) sintetisani su radi poboljšanja rastvorljivosti polimera u vodi u širokom opsegu pH i povećanja kapaciteta za inkapsulaciju lekovitih supstanci tako dobijenih PEK, koji takođe predstavljaju obećavajuće nosače.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery
T1  - Biokompatibilni nekovalentni kompleksi hitozana sa različitim polimerima - svojstva i primena kao nosača lekovitih supstanci
VL  - 70
IS  - 4
SP  - 173
EP  - 197
DO  - 10.5937/arhfarm2004173Q
ER  - 

@article{
author = "Ćirić, Ana and Krajišnik, Danina and Čalija, Bojan and Đekić, Ljiljana",
year = "2020",
abstract = "The formulation of biocompatible drug carriers based on cationic biopolymer chitosan and natural or synthetic polymers represents an important research interest. Therefore, this review aims to perceive their potential in drug delivery. The most investigated chitosan-based polymer blends are polyelectrolyte complexes (PECs) obtained by establishing ionic interactions with biocompatible   polyanions   as   alginates,   pectin,   xanthan   gum,   carrageenan, carboxymethylcellulose, and collagen. Depending on the preparation conditions, PECs could be prepared  in  versatile  forms  including  membranes/films,  hydrogel  beads,  nanoparticles,  and microparticles, to achieve controlled (e.g., extended, delayed, colon-specific and pH-dependent) drug delivery. PECs can encapsulate hydrophilic and lipophilic drug substances with different molecular  weights.  Drug  encapsulation  allows  the  preservation  of  their  structure,  activity, improvement in absorption efficiency, reduction in adverse effects and long-term stability in vitroand in  vivo. The biocompatible structures as non-covalent chitosan-based complexes could be formed also by establishing hydrogen bonds, for example with poly(vinyl alcohol). The swelling of these complexes is not pH-dependent and encapsulated drug substances are often released by already known types of diffusion. Moreover, grafted chitosan derivatives (e.g., carboxymethyl chitosan, trimethyl chitosan, acrylated chitosan) are synthesized to improve water solubility at a wide pH range and enhance the encapsulation capacity of promising PEC-based drug carriers., Formulacija biokompatibilnih nosača lekovitih supstanci na bazi katjonskog biopolimera hitozana i prirodnih ili sintetskih polimera predstavlja značajan istraživački interes. Stoga je cilj ovog rada sagledati njihovu potencijalnu primenu kao nosača lekovitih supstanci. Najistraženije blende polimera na bazi hitozana su polielektrolitni kompleksi (PEK) dobijeni uspostavljanjem jonskih interakcija sa biokompatibilnim polianjonima, npr. alginatom, pektinom, ksantan gumom, karagenanom, karboksimetilcelulozom i kolagenom. U zavisnosti od uslova pripreme, mogu se formulisati PEK u vidu membrana/filmova, hidrogelnih perli, nanočestica, mikročestica ili drugih tipova nosača, sa ciljem postizanja kontrolisanog (npr. produženog, odloženog, kolon-specifičnog i pH-zavisnog) oslobađanja lekovitih supstanci. PEK su pogodni za inkapsulaciju hidrofilnih ili lipofilnih lekovitih supstanci različitih molekulskih masa. Inkapsulacija obezbeđuje očuvanje njihove strukture, aktivnosti, poboljšanje apsorpcije, smanjenje štetnih efekata i dugoročnu stabilnost in vitro i in vivo. Biokompatibilne strukture nalik kompleksima na bazi hitozana mogu se formirati i uspostavljanjem vodoničnih veza, kao što je slučaj sa polivinil alkoholom. Njihovo bubrenje ne zavisi od pH. Inkapsulirane lekovite supstance se najčešće oslobađaju prema nekom od već poznatih tipova difuzije. Dodatno, različiti derivati hitozana (npr. karboksimetilhitozan, trimetilhitozan, akril derivati hitozana) sintetisani su radi poboljšanja rastvorljivosti polimera u vodi u širokom opsegu pH i povećanja kapaciteta za inkapsulaciju lekovitih supstanci tako dobijenih PEK, koji takođe predstavljaju obećavajuće nosače.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery, Biokompatibilni nekovalentni kompleksi hitozana sa različitim polimerima - svojstva i primena kao nosača lekovitih supstanci",
volume = "70",
number = "4",
pages = "173-197",
doi = "10.5937/arhfarm2004173Q"
}

Ćirić, A., Krajišnik, D., Čalija, B.,& Đekić, L.. (2020). Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 70(4), 173-197.
https://doi.org/10.5937/arhfarm2004173Q

Ćirić A, Krajišnik D, Čalija B, Đekić L. Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery. in Arhiv za farmaciju. 2020;70(4):173-197.
doi:10.5937/arhfarm2004173Q .

Ćirić, Ana, Krajišnik, Danina, Čalija, Bojan, Đekić, Ljiljana, "Biocompatible non-covalent complexes of chitosan and different polymers: characteristics and application in drug delivery" in Arhiv za farmaciju, 70, no. 4 (2020):173-197,
https://doi.org/10.5937/arhfarm2004173Q . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Čalija, Bojan
AU  - Medarević, Đorđe
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3527
AB  - The study elucidated the combined effect of the formulation parameters (the relative contents of the copolymer (poloxamer 407 (P407)), the cosolvent (isopropyl alcohol), and the hydrophobic drug (ibuprofen)) on gelation, applicative properties, drug solubilization capacity and release kinetics of the P407 aqueous solutions under the common conditions of storage and topical administration. The presence of ibuprofen at a therapeutic concentration of 5% enhanced the increase in micellar volume fraction and allowed the gelation of the liquid solutions at P407 concentrations ≥ 15%. Light microscopy, DSC analysis, and rheological measurements pointed that P407 gels with 15–20% of the copolymer enabled controled precipitation of amorphous ibuprofen particles (≤100 μm) in perimicellar microchannels, while gels with 25–30% of P407 as well as increased relative content of the cosolvent in perimicellar aqueous phase, had capacity for complete ibuprofen solubilization. The dissolution of the drug particles during the in vitro drug release test, maintained the drug concentration gradient between the perimicellar microchannels and the acceptor medium allowing diffusion-based sustained drug release up to 12 h. The gels with completely solubilized drug notably decrease drug release rate and the cumulative amount of the drug released. Harmonization of the investigated formulation parameters was critical to the formulation of P407 gels that could be promising drug delivery systems for sustained percutaneous delivery of the hydrophobic model drug ibuprofen with reduced frequency of administration and improved patient compliance.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug
VL  - 303
DO  - 10.1016/j.molliq.2020.112639
ER  - 

@article{
author = "Đekić, Ljiljana and Čalija, Bojan and Medarević, Đorđe",
year = "2020",
abstract = "The study elucidated the combined effect of the formulation parameters (the relative contents of the copolymer (poloxamer 407 (P407)), the cosolvent (isopropyl alcohol), and the hydrophobic drug (ibuprofen)) on gelation, applicative properties, drug solubilization capacity and release kinetics of the P407 aqueous solutions under the common conditions of storage and topical administration. The presence of ibuprofen at a therapeutic concentration of 5% enhanced the increase in micellar volume fraction and allowed the gelation of the liquid solutions at P407 concentrations ≥ 15%. Light microscopy, DSC analysis, and rheological measurements pointed that P407 gels with 15–20% of the copolymer enabled controled precipitation of amorphous ibuprofen particles (≤100 μm) in perimicellar microchannels, while gels with 25–30% of P407 as well as increased relative content of the cosolvent in perimicellar aqueous phase, had capacity for complete ibuprofen solubilization. The dissolution of the drug particles during the in vitro drug release test, maintained the drug concentration gradient between the perimicellar microchannels and the acceptor medium allowing diffusion-based sustained drug release up to 12 h. The gels with completely solubilized drug notably decrease drug release rate and the cumulative amount of the drug released. Harmonization of the investigated formulation parameters was critical to the formulation of P407 gels that could be promising drug delivery systems for sustained percutaneous delivery of the hydrophobic model drug ibuprofen with reduced frequency of administration and improved patient compliance.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug",
volume = "303",
doi = "10.1016/j.molliq.2020.112639"
}

Đekić, L., Čalija, B.,& Medarević, Đ.. (2020). Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug. in Journal of Molecular Liquids
Elsevier B.V.., 303.
https://doi.org/10.1016/j.molliq.2020.112639

Đekić L, Čalija B, Medarević Đ. Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug. in Journal of Molecular Liquids. 2020;303.
doi:10.1016/j.molliq.2020.112639 .

Đekić, Ljiljana, Čalija, Bojan, Medarević, Đorđe, "Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug" in Journal of Molecular Liquids, 303 (2020),
https://doi.org/10.1016/j.molliq.2020.112639 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Medarević, Đorđe
AU  - Čalija, Bojan
AU  - Dobričić, Vladimir
AU  - Mitrić, Miodrag
AU  - Đekić, Ljiljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3524
AB  - This study investigated the combined influence of pH adjusting agent type (hydrochloric, acetic or lactic acid) and initial pH value (3.6, 4.6, and 5.6) on formation of biocompatible chitosan/xanthan polyelectrolyte complexes (PECs), their characteristics in solid state and influence on in vitro ibuprofen release kinetics. Conductivity measurements and rheological characterization revealed generally higher extent of ionic interactions in PEC dispersions comprising acetic acid and at pH 3.6. Acid type and pH affected significantly the yield and particle size (100–250 μm) of the dried PECs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD) analysis of the solid PECs confirmed exclusively physical (ionic, hydrogen bonds) interactions between chitosan and xanthan gum. PECs prepared with acetic acid at pH 4.6 and 5.6 had enhanced rehydration ability in phosphate buffer pH 7.2, and at PEC-to-drug mass ratio up to 1:2, enabled extended ibuprofen release from hard capsules during 10 h.
PB  - Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics
VL  - 148
SP  - 942
EP  - 955
DO  - 10.1016/j.ijbiomac.2020.01.138
ER  - 

@article{
author = "Ćirić, Ana and Medarević, Đorđe and Čalija, Bojan and Dobričić, Vladimir and Mitrić, Miodrag and Đekić, Ljiljana",
year = "2020",
abstract = "This study investigated the combined influence of pH adjusting agent type (hydrochloric, acetic or lactic acid) and initial pH value (3.6, 4.6, and 5.6) on formation of biocompatible chitosan/xanthan polyelectrolyte complexes (PECs), their characteristics in solid state and influence on in vitro ibuprofen release kinetics. Conductivity measurements and rheological characterization revealed generally higher extent of ionic interactions in PEC dispersions comprising acetic acid and at pH 3.6. Acid type and pH affected significantly the yield and particle size (100–250 μm) of the dried PECs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD) analysis of the solid PECs confirmed exclusively physical (ionic, hydrogen bonds) interactions between chitosan and xanthan gum. PECs prepared with acetic acid at pH 4.6 and 5.6 had enhanced rehydration ability in phosphate buffer pH 7.2, and at PEC-to-drug mass ratio up to 1:2, enabled extended ibuprofen release from hard capsules during 10 h.",
publisher = "Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics",
volume = "148",
pages = "942-955",
doi = "10.1016/j.ijbiomac.2020.01.138"
}

Ćirić, A., Medarević, Đ., Čalija, B., Dobričić, V., Mitrić, M.,& Đekić, L.. (2020). Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics. in International Journal of Biological Macromolecules
Elsevier B.V.., 148, 942-955.
https://doi.org/10.1016/j.ijbiomac.2020.01.138

Ćirić A, Medarević Đ, Čalija B, Dobričić V, Mitrić M, Đekić L. Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics. in International Journal of Biological Macromolecules. 2020;148:942-955.
doi:10.1016/j.ijbiomac.2020.01.138 .

Ćirić, Ana, Medarević, Đorđe, Čalija, Bojan, Dobričić, Vladimir, Mitrić, Miodrag, Đekić, Ljiljana, "Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics" in International Journal of Biological Macromolecules, 148 (2020):942-955,
https://doi.org/10.1016/j.ijbiomac.2020.01.138 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milašinović, Nikola
AU  - Daković, Aleksandra
AU  - Trifković, Kata
AU  - Stojanović, Jovica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3478
AB  - This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics
PB  - Wiley Periodicals, Inc.
T2  - Journal of Applied Polymer Science
T1  - Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
VL  - 137
IS  - 8
DO  - 10.1002/app.48406
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Milašinović, Nikola and Daković, Aleksandra and Trifković, Kata and Stojanović, Jovica",
year = "2020",
abstract = "This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics",
publisher = "Wiley Periodicals, Inc.",
journal = "Journal of Applied Polymer Science",
title = "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass",
volume = "137",
number = "8",
doi = "10.1002/app.48406"
}

Čalija, B., Milić, J., Milašinović, N., Daković, A., Trifković, K.,& Stojanović, J.. (2020). Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science
Wiley Periodicals, Inc.., 137(8).
https://doi.org/10.1002/app.48406

Čalija B, Milić J, Milašinović N, Daković A, Trifković K, Stojanović J. Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science. 2020;137(8).
doi:10.1002/app.48406 .

Čalija, Bojan, Milić, Jela, Milašinović, Nikola, Daković, Aleksandra, Trifković, Kata, Stojanović, Jovica, "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass" in Journal of Applied Polymer Science, 137, no. 8 (2020),
https://doi.org/10.1002/app.48406 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milašinović, Nikola
AU  - Daković, Aleksandra
AU  - Trifković, Kata
AU  - Stojanović, Jovica
AU  - Krajišnik, Danina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3476
AB  - This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.
PB  - Wiley Periodicals, Inc.
T2  - Journal of Applied Polymer Science
T1  - Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
VL  - 137
IS  - 8
DO  - 10.1002/app.48406
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Milašinović, Nikola and Daković, Aleksandra and Trifković, Kata and Stojanović, Jovica and Krajišnik, Danina",
year = "2020",
abstract = "This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.",
publisher = "Wiley Periodicals, Inc.",
journal = "Journal of Applied Polymer Science",
title = "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass",
volume = "137",
number = "8",
doi = "10.1002/app.48406"
}

Čalija, B., Milić, J., Milašinović, N., Daković, A., Trifković, K., Stojanović, J.,& Krajišnik, D.. (2020). Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science
Wiley Periodicals, Inc.., 137(8).
https://doi.org/10.1002/app.48406

Čalija B, Milić J, Milašinović N, Daković A, Trifković K, Stojanović J, Krajišnik D. Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science. 2020;137(8).
doi:10.1002/app.48406 .

Čalija, Bojan, Milić, Jela, Milašinović, Nikola, Daković, Aleksandra, Trifković, Kata, Stojanović, Jovica, Krajišnik, Danina, "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass" in Journal of Applied Polymer Science, 137, no. 8 (2020),
https://doi.org/10.1002/app.48406 . .

TY  - JOUR
AU  - Mladenović, Nataša
AU  - Kljajević,  Ljiljana
AU  - Nenadović, Snežana
AU  - Ivanović, Marija
AU  - Čalija, Bojan
AU  - Gulicovski, Jelena
AU  - Trivunac, Katarina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3276
AB  - Fundamental research of inorganic polymers prepared from available aluminosilicate precursors represent an innovative class of materials characterized by low energy consumption for production. This is just one of the reasons why their use is focused in protecting the environment for removing of heavy metals from aqueous solutions. The concentration of hydroxide as activator solution plays an important role in the geopolymerization process. The present study examined the use of geopolymer materials, obtained in reaction of geopolymerizations of metakaolin as precursor activated with NaOH concentration 2.0, 4.0, 6.0 and 8.0 mol/dm3 for removal of cadmium ions from aqueous solutions. The structure and properties of the obtained geopolymer samples were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM) and diffuse reflection infrared spectroscopy (DRIFTS). To investigate the surface charge of geopolymers the zeta potential measurements were performed. Batch adsorption experiments conducted at room temperature (23 ± 1 °C) showed that the adsorption pattern followed the Freundlich isotherm model. The maximum removal of cadmium obtained from batch studies was 84.1% for GP6M at pH ≈ 6.7. The results generally showed that geopolymer samples could be considered as a potential adsorbent for cadmium removal from aqueous solutions.
PB  - Springer Nature
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - The Applications of New Inorganic Polymer for Adsorption Cadmium from Waste Water
VL  - 30
IS  - 2
SP  - 554
EP  - 563
DO  - 10.1007/s10904-019-01215-y
ER  - 

@article{
author = "Mladenović, Nataša and Kljajević,  Ljiljana and Nenadović, Snežana and Ivanović, Marija and Čalija, Bojan and Gulicovski, Jelena and Trivunac, Katarina",
year = "2020",
abstract = "Fundamental research of inorganic polymers prepared from available aluminosilicate precursors represent an innovative class of materials characterized by low energy consumption for production. This is just one of the reasons why their use is focused in protecting the environment for removing of heavy metals from aqueous solutions. The concentration of hydroxide as activator solution plays an important role in the geopolymerization process. The present study examined the use of geopolymer materials, obtained in reaction of geopolymerizations of metakaolin as precursor activated with NaOH concentration 2.0, 4.0, 6.0 and 8.0 mol/dm3 for removal of cadmium ions from aqueous solutions. The structure and properties of the obtained geopolymer samples were studied by X-ray diffraction (XRD), scanning electron microscopy (SEM) and diffuse reflection infrared spectroscopy (DRIFTS). To investigate the surface charge of geopolymers the zeta potential measurements were performed. Batch adsorption experiments conducted at room temperature (23 ± 1 °C) showed that the adsorption pattern followed the Freundlich isotherm model. The maximum removal of cadmium obtained from batch studies was 84.1% for GP6M at pH ≈ 6.7. The results generally showed that geopolymer samples could be considered as a potential adsorbent for cadmium removal from aqueous solutions.",
publisher = "Springer Nature",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "The Applications of New Inorganic Polymer for Adsorption Cadmium from Waste Water",
volume = "30",
number = "2",
pages = "554-563",
doi = "10.1007/s10904-019-01215-y"
}

Mladenović, N., Kljajević, L., Nenadović, S., Ivanović, M., Čalija, B., Gulicovski, J.,& Trivunac, K.. (2020). The Applications of New Inorganic Polymer for Adsorption Cadmium from Waste Water. in Journal of Inorganic and Organometallic Polymers and Materials
Springer Nature., 30(2), 554-563.
https://doi.org/10.1007/s10904-019-01215-y

Mladenović N, Kljajević L, Nenadović S, Ivanović M, Čalija B, Gulicovski J, Trivunac K. The Applications of New Inorganic Polymer for Adsorption Cadmium from Waste Water. in Journal of Inorganic and Organometallic Polymers and Materials. 2020;30(2):554-563.
doi:10.1007/s10904-019-01215-y .

Mladenović, Nataša, Kljajević,  Ljiljana, Nenadović, Snežana, Ivanović, Marija, Čalija, Bojan, Gulicovski, Jelena, Trivunac, Katarina, "The Applications of New Inorganic Polymer for Adsorption Cadmium from Waste Water" in Journal of Inorganic and Organometallic Polymers and Materials, 30, no. 2 (2020):554-563,
https://doi.org/10.1007/s10904-019-01215-y . .

TY  - CONF
AU  - Mitrović, Jelena
AU  - Divović, Branka
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Vulić, Jelena P.
AU  - Knutson, Daniel E.
AU  - Cook, James M.
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5542
AB  - Deuterated pyrazoloquinolinones, GABAn receptor ligands, are being investigated for neuropsychiatric disorders treatment. Among others, DK-l-56-1 (7-Methoxy-2-(4-methoxy-d3- p h e n yl ) - 2, 5 -d i h yd ro - 3 H - py r azolo -14,3 -clq u i n o I i n - 3-one) was chosen as the lead component [1]. Because of its low solubility in water (6.27 t0.74 pg/ml) nanosuspension formulation for prospective parenteral administration was carried out [2]. ...
C3  - 10th International Congress Nanotechnology in Medicine & Biology, 15 - 17. April 2019, Graz / Austria
T1  - Nanonization of new patent protected substances - carrier selection for preclinical research: physicochemical and in vivo behavioral characterization
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5542
ER  - 

@conference{
author = "Mitrović, Jelena and Divović, Branka and Dobričić, Vladimir and Čalija, Bojan and Vulić, Jelena P. and Knutson, Daniel E. and Cook, James M. and Savić, Miroslav and Savić, Snežana",
year = "2019",
abstract = "Deuterated pyrazoloquinolinones, GABAn receptor ligands, are being investigated for neuropsychiatric disorders treatment. Among others, DK-l-56-1 (7-Methoxy-2-(4-methoxy-d3- p h e n yl ) - 2, 5 -d i h yd ro - 3 H - py r azolo -14,3 -clq u i n o I i n - 3-one) was chosen as the lead component [1]. Because of its low solubility in water (6.27 t0.74 pg/ml) nanosuspension formulation for prospective parenteral administration was carried out [2]. ...",
journal = "10th International Congress Nanotechnology in Medicine & Biology, 15 - 17. April 2019, Graz / Austria",
title = "Nanonization of new patent protected substances - carrier selection for preclinical research: physicochemical and in vivo behavioral characterization",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5542"
}

Mitrović, J., Divović, B., Dobričić, V., Čalija, B., Vulić, J. P., Knutson, D. E., Cook, J. M., Savić, M.,& Savić, S.. (2019). Nanonization of new patent protected substances - carrier selection for preclinical research: physicochemical and in vivo behavioral characterization. in 10th International Congress Nanotechnology in Medicine & Biology, 15 - 17. April 2019, Graz / Austria.
https://hdl.handle.net/21.15107/rcub_farfar_5542

Mitrović J, Divović B, Dobričić V, Čalija B, Vulić JP, Knutson DE, Cook JM, Savić M, Savić S. Nanonization of new patent protected substances - carrier selection for preclinical research: physicochemical and in vivo behavioral characterization. in 10th International Congress Nanotechnology in Medicine & Biology, 15 - 17. April 2019, Graz / Austria. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_5542 .

Mitrović, Jelena, Divović, Branka, Dobričić, Vladimir, Čalija, Bojan, Vulić, Jelena P., Knutson, Daniel E., Cook, James M., Savić, Miroslav, Savić, Snežana, "Nanonization of new patent protected substances - carrier selection for preclinical research: physicochemical and in vivo behavioral characterization" in 10th International Congress Nanotechnology in Medicine & Biology, 15 - 17. April 2019, Graz / Austria (2019),
https://hdl.handle.net/21.15107/rcub_farfar_5542 .

TY  - JOUR
AU  - Savić, Vedrana
AU  - Ilić, Tanja
AU  - Nikolić, Ines
AU  - Marković, Bojan
AU  - Čalija, Bojan
AU  - Cekić, Nebojša
AU  - Savić, Snežana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3454
AB  - Nanostructured lipid carriers (NLC) and nanoemulsions (NE) are colloid carriers which could improve dermal delivery of tacrolimus. The aims of this study were to evaluate effects of different formulation and process parameters on physicochemical characteristics and stability of lecithin-based NLC with glyceryl palmitostearate as solid and propylene glycol monocaprylate as liquid lipid and to compare the influence of different inner structure of tacrolimus-loaded NLC and corresponding NE on physicochemical characteristics, stability, entrapment efficiency, in vitro drug release and overall skin performance. Solid/liquid lipid ratio, total amount of lipids, homogenization pressure and cooling after the preparation were identified as critical variables in NLC development. Moreover, tacrolimus-loaded NLC emerged as more stabile carrier than NE. Differential stripping performed on porcine ear skin revealed significantly higher tacrolimus amount in stratum corneum from nanocarriers compared to referent ointment (Protopic®). Similarly the highest amount of tacrolimus in hair follicles was obtained using NLC (268.54 ± 92.38 ng/cm2), followed by NE (128.17 ± 48.87 ng/cm2) and Protopic® (77.61 ± 43.25 ng/cm2). Contrary, the highest permeation rate through full-thickness porcine ear skin was observed for Protopic®, implying that the selection of experimental setup is critical for reliable skin performance assessment. Overall, developed NLC could be suggested as promising carrier in a form of lotion for tacrolimus dermal delivery.
T2  - International Journal of Pharmaceutics
T1  - Tacrolimus-loaded lecithin-based nanostructured lipid carrier and nanoemulsion with propylene glycol monocaprylate as a liquid lipid: Formulation characterization and assessment of dermal delivery compared to referent ointment
VL  - 569
SP  - 1
EP  - 11
DO  - 10.1016/j.ijpharm.2019.118624
ER  - 

@article{
author = "Savić, Vedrana and Ilić, Tanja and Nikolić, Ines and Marković, Bojan and Čalija, Bojan and Cekić, Nebojša and Savić, Snežana",
year = "2019",
abstract = "Nanostructured lipid carriers (NLC) and nanoemulsions (NE) are colloid carriers which could improve dermal delivery of tacrolimus. The aims of this study were to evaluate effects of different formulation and process parameters on physicochemical characteristics and stability of lecithin-based NLC with glyceryl palmitostearate as solid and propylene glycol monocaprylate as liquid lipid and to compare the influence of different inner structure of tacrolimus-loaded NLC and corresponding NE on physicochemical characteristics, stability, entrapment efficiency, in vitro drug release and overall skin performance. Solid/liquid lipid ratio, total amount of lipids, homogenization pressure and cooling after the preparation were identified as critical variables in NLC development. Moreover, tacrolimus-loaded NLC emerged as more stabile carrier than NE. Differential stripping performed on porcine ear skin revealed significantly higher tacrolimus amount in stratum corneum from nanocarriers compared to referent ointment (Protopic®). Similarly the highest amount of tacrolimus in hair follicles was obtained using NLC (268.54 ± 92.38 ng/cm2), followed by NE (128.17 ± 48.87 ng/cm2) and Protopic® (77.61 ± 43.25 ng/cm2). Contrary, the highest permeation rate through full-thickness porcine ear skin was observed for Protopic®, implying that the selection of experimental setup is critical for reliable skin performance assessment. Overall, developed NLC could be suggested as promising carrier in a form of lotion for tacrolimus dermal delivery.",
journal = "International Journal of Pharmaceutics",
title = "Tacrolimus-loaded lecithin-based nanostructured lipid carrier and nanoemulsion with propylene glycol monocaprylate as a liquid lipid: Formulation characterization and assessment of dermal delivery compared to referent ointment",
volume = "569",
pages = "1-11",
doi = "10.1016/j.ijpharm.2019.118624"
}

Savić, V., Ilić, T., Nikolić, I., Marković, B., Čalija, B., Cekić, N.,& Savić, S.. (2019). Tacrolimus-loaded lecithin-based nanostructured lipid carrier and nanoemulsion with propylene glycol monocaprylate as a liquid lipid: Formulation characterization and assessment of dermal delivery compared to referent ointment. in International Journal of Pharmaceutics, 569, 1-11.
https://doi.org/10.1016/j.ijpharm.2019.118624

Savić V, Ilić T, Nikolić I, Marković B, Čalija B, Cekić N, Savić S. Tacrolimus-loaded lecithin-based nanostructured lipid carrier and nanoemulsion with propylene glycol monocaprylate as a liquid lipid: Formulation characterization and assessment of dermal delivery compared to referent ointment. in International Journal of Pharmaceutics. 2019;569:1-11.
doi:10.1016/j.ijpharm.2019.118624 .

Savić, Vedrana, Ilić, Tanja, Nikolić, Ines, Marković, Bojan, Čalija, Bojan, Cekić, Nebojša, Savić, Snežana, "Tacrolimus-loaded lecithin-based nanostructured lipid carrier and nanoemulsion with propylene glycol monocaprylate as a liquid lipid: Formulation characterization and assessment of dermal delivery compared to referent ointment" in International Journal of Pharmaceutics, 569 (2019):1-11,
https://doi.org/10.1016/j.ijpharm.2019.118624 . .

TY  - CONF
AU  - Račić, Anđelka
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Dobričić, Vladimir
AU  - Krajišnik, Danina
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5480
AB  - Ketotifen hidrogenfumarat (KF) je selektivni, nekompetitivni antagonist histamina (H1-
receptori) koji se koristi u lokalnoj terapiji alergijskog rinitisa i konjuktivitisa. Hitozan (H)
i hidroksipropil guar guma (HP GG) su biokompatibilni i biodegradabilni polimeri koji u
oftalmološkim preparatima zahvaljujući povećanju viskoziteta i mukoadhezivnim osobinama,
omogućavaju duži kontakt aktivne supstance sa rožnjačom i poboljšanje bioraspoloživosti.
Cilj ove studije bila su formulaciona i funkcionalna ispitivanja rastvora KF (0,025% m/v) za
oftalmološku primjenu koji sadrže H srednje molekulske mase (0,5% m/v) (uzorak F1), HP GG
(0,25% m/v) (uzorak F2) i njihovu kombinaciju (0,25 % m/v H/0,25% m/v HP GG) (uzorak F3) kao
modifikatore viskoziteta. Oftalmološki rastvori su pripremljeni miješanjem vehikuluma koji su
sadržavali polisaharide i pomoćne supstance (dibazni natrijum-fosfat, boraks i benzalkonijum-
hlorid) sa vodenim rastvorima KF. Izrađenim uzorcima ispitana je pH vrijednost, bistrina,
osmolalnost i sadržaj ljekovite supstance. Reološka karakterizacija sprovedena je na svježim
uzorcima na 20 °C i 34 °C (nakon razblaživanja sa vještačkom suznom tečnosti (VST) u odnosu
40:7) prije i nakon sterilizacije vodenom parom pod pritiskom. Sadržaj KF analiziran je HPLC
metodom.
Za sve ispitivane formulacije pH vrijednost, bistrina i osmolalnost bili su u prihvatljivom opsegu
za oftalmološke preparate. Vrijednosti viskoziteta (pri 20 ºC/100 s-1) bile su u opsegu od 13,2
mPa·s (F1) do 21 mPa·s (F3). Nakon razblaživanja sa VST i procesa sterilizacije, promjene
viskoziteta bile su manje od ±10% u odnosu na početne vrijednosti, za sve ispitivane formulacije.
Sadržaj KF nakon tri mjeseca bio je u opsegu 88-100% kod sterilisanih i nesterilisanih uzoraka.
Uspješno su formulisani viskozni rastvori KF za oftalmološku primjenu koji sadrže modifikatore
viskoziteta tipa polisaharida. Reproduktivni HPLC rezultati ukazuju na stabilnost KF i nakon
sterilizacije. Buduća istraživanja imaće za cilj ispitivanje lijek-polimer interakcija, uticaja izbora
pomoćnih supstanci kao i postupka izrade na funkcionalne karakteristike i stabilnost ispitivanih
rastvora za oftalmološku primjenu.
AB  - Ketotifen hydrogen fumarate (KF) is a selective and non-competitive histamine antagonist (H1-receptor) used topically in the treatment of allergic rhinitis and conjunctivitis. Chitosan (CH) and hydroxypropyl guar gum (HP GG), biocompatible and biodegradable polymers as mucoadhesive and viscosity increasing agents in ophthalmic products enable longer contact of active ingredient and the corneal surface, enhancing its bioavailability. The purpose of this study was formulation and functionality assessment of KF ophthalmic solutions (0.025% w/v) containing medium molecular weight CH (0.5% w/v) (sample F1), HP GG (0.25% w/v) (sample F2) and their combination (0.25% w/v CH/0.25% w/v HP GG) (sample F3) as viscosity modifiers. The ophthalmic solutions were prepared by mixing of solutions containing these polysaccharides, auxiliary substances (dibasic sodium phosphate, borax and benzalkonium chloride) and aqueous solutions of KF. The samples were evaluated for pH, clarity, osmolality and drug content. Rheological characterization was performed on fresh samples at 20 °C and 34 °C (after addition of simulated tear fluid (STF) in a ratio 40:7), before and after steam sterilization. The content of KF was analyzed by HPLC method. The pH, clarity and osmolality of all the tested formulations were within the acceptable range for ophthalmic preparations. The viscosity values (at 20 ºC/100 s-1) were in the range from 13.2 mPa·s (F1) to 21 mPa·s (F3). After dilution with STF and steam sterilization, changes of viscosity deviated less than ±10% compared with initial values for all tested vehicles. The drug content after 3 months was within range 88-100%, for both sterilized and non-sterilized samples. KF was successfully formulated in viscous ophthalmic solutions containing polysaccharidebased viscosity modifiers. Reproducible HPLC data revealed stability of KF after steam sterilization. Future research will be focused on investigations of drug-polymer interactions and influence of auxiliary substances selection alongside with preparation procedure on functional properties and ophthalmic solutions stability.
PB  - Prisma - korporativne komunikacije
PB  - Farmaceutska komora Crne Gore
C3  - Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka
T1  - Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta
T1  - Formulation and functionality assessment of ketotifen fumarate ophthalmic solutions containing polysaccharide-based viscosity modifiers
SP  - 192
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5480
ER  - 

@conference{
author = "Račić, Anđelka and Čalija, Bojan and Milić, Jela and Dobričić, Vladimir and Krajišnik, Danina",
year = "2019",
abstract = "Ketotifen hidrogenfumarat (KF) je selektivni, nekompetitivni antagonist histamina (H1-
receptori) koji se koristi u lokalnoj terapiji alergijskog rinitisa i konjuktivitisa. Hitozan (H)
i hidroksipropil guar guma (HP GG) su biokompatibilni i biodegradabilni polimeri koji u
oftalmološkim preparatima zahvaljujući povećanju viskoziteta i mukoadhezivnim osobinama,
omogućavaju duži kontakt aktivne supstance sa rožnjačom i poboljšanje bioraspoloživosti.
Cilj ove studije bila su formulaciona i funkcionalna ispitivanja rastvora KF (0,025% m/v) za
oftalmološku primjenu koji sadrže H srednje molekulske mase (0,5% m/v) (uzorak F1), HP GG
(0,25% m/v) (uzorak F2) i njihovu kombinaciju (0,25 % m/v H/0,25% m/v HP GG) (uzorak F3) kao
modifikatore viskoziteta. Oftalmološki rastvori su pripremljeni miješanjem vehikuluma koji su
sadržavali polisaharide i pomoćne supstance (dibazni natrijum-fosfat, boraks i benzalkonijum-
hlorid) sa vodenim rastvorima KF. Izrađenim uzorcima ispitana je pH vrijednost, bistrina,
osmolalnost i sadržaj ljekovite supstance. Reološka karakterizacija sprovedena je na svježim
uzorcima na 20 °C i 34 °C (nakon razblaživanja sa vještačkom suznom tečnosti (VST) u odnosu
40:7) prije i nakon sterilizacije vodenom parom pod pritiskom. Sadržaj KF analiziran je HPLC
metodom.
Za sve ispitivane formulacije pH vrijednost, bistrina i osmolalnost bili su u prihvatljivom opsegu
za oftalmološke preparate. Vrijednosti viskoziteta (pri 20 ºC/100 s-1) bile su u opsegu od 13,2
mPa·s (F1) do 21 mPa·s (F3). Nakon razblaživanja sa VST i procesa sterilizacije, promjene
viskoziteta bile su manje od ±10% u odnosu na početne vrijednosti, za sve ispitivane formulacije.
Sadržaj KF nakon tri mjeseca bio je u opsegu 88-100% kod sterilisanih i nesterilisanih uzoraka.
Uspješno su formulisani viskozni rastvori KF za oftalmološku primjenu koji sadrže modifikatore
viskoziteta tipa polisaharida. Reproduktivni HPLC rezultati ukazuju na stabilnost KF i nakon
sterilizacije. Buduća istraživanja imaće za cilj ispitivanje lijek-polimer interakcija, uticaja izbora
pomoćnih supstanci kao i postupka izrade na funkcionalne karakteristike i stabilnost ispitivanih
rastvora za oftalmološku primjenu., Ketotifen hydrogen fumarate (KF) is a selective and non-competitive histamine antagonist (H1-receptor) used topically in the treatment of allergic rhinitis and conjunctivitis. Chitosan (CH) and hydroxypropyl guar gum (HP GG), biocompatible and biodegradable polymers as mucoadhesive and viscosity increasing agents in ophthalmic products enable longer contact of active ingredient and the corneal surface, enhancing its bioavailability. The purpose of this study was formulation and functionality assessment of KF ophthalmic solutions (0.025% w/v) containing medium molecular weight CH (0.5% w/v) (sample F1), HP GG (0.25% w/v) (sample F2) and their combination (0.25% w/v CH/0.25% w/v HP GG) (sample F3) as viscosity modifiers. The ophthalmic solutions were prepared by mixing of solutions containing these polysaccharides, auxiliary substances (dibasic sodium phosphate, borax and benzalkonium chloride) and aqueous solutions of KF. The samples were evaluated for pH, clarity, osmolality and drug content. Rheological characterization was performed on fresh samples at 20 °C and 34 °C (after addition of simulated tear fluid (STF) in a ratio 40:7), before and after steam sterilization. The content of KF was analyzed by HPLC method. The pH, clarity and osmolality of all the tested formulations were within the acceptable range for ophthalmic preparations. The viscosity values (at 20 ºC/100 s-1) were in the range from 13.2 mPa·s (F1) to 21 mPa·s (F3). After dilution with STF and steam sterilization, changes of viscosity deviated less than ±10% compared with initial values for all tested vehicles. The drug content after 3 months was within range 88-100%, for both sterilized and non-sterilized samples. KF was successfully formulated in viscous ophthalmic solutions containing polysaccharidebased viscosity modifiers. Reproducible HPLC data revealed stability of KF after steam sterilization. Future research will be focused on investigations of drug-polymer interactions and influence of auxiliary substances selection alongside with preparation procedure on functional properties and ophthalmic solutions stability.",
publisher = "Prisma - korporativne komunikacije, Farmaceutska komora Crne Gore",
journal = "Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka",
title = "Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta, Formulation and functionality assessment of ketotifen fumarate ophthalmic solutions containing polysaccharide-based viscosity modifiers",
pages = "192-193",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5480"
}

Račić, A., Čalija, B., Milić, J., Dobričić, V.,& Krajišnik, D.. (2019). Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka
Prisma - korporativne komunikacije., 192-193.
https://hdl.handle.net/21.15107/rcub_farfar_5480

Račić A, Čalija B, Milić J, Dobričić V, Krajišnik D. Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka. 2019;:192-193.
https://hdl.handle.net/21.15107/rcub_farfar_5480 .

Račić, Anđelka, Čalija, Bojan, Milić, Jela, Dobričić, Vladimir, Krajišnik, Danina, "Formulaciona i funkcionalna ispitivanja rastvora ketotifen-fumarata za oftalmološku primjenu sa polisaharidnim modifikatorima viskoziteta" in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09. - 12. maj 2019. godine, Budva, Bečići, Crna Gora, Knjiga sažetaka (2019):192-193,
https://hdl.handle.net/21.15107/rcub_farfar_5480 .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Krajišnik, Danina
AU  - Milić, Jela
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3651
AB  - Owing to its safety, wide distribution, and unique physicochemical properties, water is indispensable in pharmaceutical industry as excipient, solvent for extraction, production of active ingredients and analytical reagents, cleaning and heat-transfer agent. Various water types are used for production of pharmaceutical preparations and the water quality requirements depend on characteristics and use of final pharmaceutical preparation and production stage at which is water used. This paper summarizes regulatory requirements related to water for pharmaceutical use and systems for water production, storage and distribution in pharmaceutical industry, with focus on current regulation changes in this field. An overview on types of water for pharmaceutical use is provided, especially that official in The European and The United States Pharmacopoeia, including their quality requirements, usage and production methods.
AB  - Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda je nezamenjiva u farmaceutskoj industriji kao ekscipijens, sredstvo za proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan je osobinama i namenom finalnog farmaceutskog preparata i fazom u postupku njegove proizvodnje u kojoj se voda koristi. U ovom radu su predstavljeni regulatorni zahtevi za vode za farmaceutsku upotrebu i sisteme za proizvodnju, distribuciju i čuvanje vode u farmaceutskoj industriji, sa posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu, sa fokusom na vode oficijalne u Evropskoj i Američkoj farmakopeji, uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - Watertypesfor pharmaceuticaland quality requirementsuse – importance
T1  - Voda za farmaceutsku upotrebu – značaj, vrste i zahtevi za kvalitet
T1  - Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda  je  nezamenjiva  u  farmaceutskoj  industriji  kao  ekscipijens,  sredstvo  za  proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan  je  osobinama  i  namenom  finalnog  farmaceutskog  preparata  i  fazom  u  postupku njegove proizvodnje u kojoj se voda koristi.  U  ovom  radu  su  predstavljeni  regulatorni  zahtevi za  vode  za  farmaceutsku  upotrebu  i sisteme  za  proizvodnju,  distribuciju  i  čuvanje  vode  u  farmaceutskoj  industriji,  sa  posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu,  sa  fokusom  na  vode  oficinalne  u  Evropskoj  i  Američkoj  farmakopeji,  uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.
VL  - 69
IS  - 2
SP  - 90
EP  - 115
DO  - 10.5937/arhfarm1902090Q
ER  - 

@article{
author = "Čalija, Bojan and Krajišnik, Danina and Milić, Jela",
year = "2019",
abstract = "Owing to its safety, wide distribution, and unique physicochemical properties, water is indispensable in pharmaceutical industry as excipient, solvent for extraction, production of active ingredients and analytical reagents, cleaning and heat-transfer agent. Various water types are used for production of pharmaceutical preparations and the water quality requirements depend on characteristics and use of final pharmaceutical preparation and production stage at which is water used. This paper summarizes regulatory requirements related to water for pharmaceutical use and systems for water production, storage and distribution in pharmaceutical industry, with focus on current regulation changes in this field. An overview on types of water for pharmaceutical use is provided, especially that official in The European and The United States Pharmacopoeia, including their quality requirements, usage and production methods., Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda je nezamenjiva u farmaceutskoj industriji kao ekscipijens, sredstvo za proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan je osobinama i namenom finalnog farmaceutskog preparata i fazom u postupku njegove proizvodnje u kojoj se voda koristi. U ovom radu su predstavljeni regulatorni zahtevi za vode za farmaceutsku upotrebu i sisteme za proizvodnju, distribuciju i čuvanje vode u farmaceutskoj industriji, sa posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu, sa fokusom na vode oficijalne u Evropskoj i Američkoj farmakopeji, uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Watertypesfor pharmaceuticaland quality requirementsuse – importance, Voda za farmaceutsku upotrebu – značaj, vrste i zahtevi za kvalitet, Zahvaljujući bezbednosti, rasprostranjenosti i jedinstvenim fizičko-hemijskim osobinama, voda  je  nezamenjiva  u  farmaceutskoj  industriji  kao  ekscipijens,  sredstvo  za  proizvodnju aktivnih supstanci i analitičkih reagenasa, ekstrakciju, čišćenje/pranje i razmenu toplote. Vode različitog kvaliteta se koriste za proizvodnju farmaceutskih preparata, a zahtevani kvalitet vode definisan  je  osobinama  i  namenom  finalnog  farmaceutskog  preparata  i  fazom  u  postupku njegove proizvodnje u kojoj se voda koristi.  U  ovom  radu  su  predstavljeni  regulatorni  zahtevi za  vode  za  farmaceutsku  upotrebu  i sisteme  za  proizvodnju,  distribuciju  i  čuvanje  vode  u  farmaceutskoj  industriji,  sa  posebnim osvrtom na aktuelne izmene propisa u ovoj oblasti. Prikazan je i pregled voda za farmaceutsku upotrebu,  sa  fokusom  na  vode  oficinalne  u  Evropskoj  i  Američkoj  farmakopeji,  uključujući zahteve za njihov kvalitet, namenu i postupke proizvodnje.",
volume = "69",
number = "2",
pages = "90-115",
doi = "10.5937/arhfarm1902090Q"
}

Čalija, B., Krajišnik, D.,& Milić, J.. (2019). Watertypesfor pharmaceuticaland quality requirementsuse – importance. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 69(2), 90-115.
https://doi.org/10.5937/arhfarm1902090Q

Čalija B, Krajišnik D, Milić J. Watertypesfor pharmaceuticaland quality requirementsuse – importance. in Arhiv za farmaciju. 2019;69(2):90-115.
doi:10.5937/arhfarm1902090Q .

Čalija, Bojan, Krajišnik, Danina, Milić, Jela, "Watertypesfor pharmaceuticaland quality requirementsuse – importance" in Arhiv za farmaciju, 69, no. 2 (2019):90-115,
https://doi.org/10.5937/arhfarm1902090Q . .

TY  - JOUR
AU  - Račić, Anđelka
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milasinvić, Nikola
AU  - Krajišnik, Danina
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3330
AB  - Incorporation of mucoadhesive polymers is one of the first efficient modification of conventional liquid ophthalmic formulations in order to prolong the residence time at ocular surface. The aim of this work was formulation of viscous ophthalmic vehicles/ocular lubricants containing derivative of guar gum, chitosan and cellulose derivatives (hypromellose and hydroxyethylcellulose) alone or in combination. Optimized formulations were developed by choosing appropriate polymer or combination of polymers in suitable concentrations with adequate physicochemical, rheological and mucoadhesive properties. Influence of the addition of simulated tear fluid, steam sterilization and storage on clarity, pH value and rheological properties of different formulations were evaluated. The compounded vehicles were compared with commercially available ocular lubricants containing similar polymers. It was observed that viscosities of commercially available eye drops were above the optimal viscosity range. The mucoadhesive potential of the vehicles, rheological synergism and type of polymermucin interaction were determined. Combination of chitosan and guar gum exhibited optimal physicochemical, rheological and mucoadhesive properties and had a potential for the longest retention time in tear film. Considering the simplicity of its preparation and stability (over a period of one-month storage), this vehicle could be used for extemporaneously compounded ocular preparations for specific needs of individual patients.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Drug Delivery Science and Technology
T1  - Development of polysaccharide-based mucoadhesive ophthalmic lubricating vehicles: The effect of different polymers on physicochemical properties and functionality
VL  - 49
SP  - 50
EP  - 57
DO  - 10.1016/j.jddst.2018.10.034
ER  - 

@article{
author = "Račić, Anđelka and Čalija, Bojan and Milić, Jela and Milasinvić, Nikola and Krajišnik, Danina",
year = "2019",
abstract = "Incorporation of mucoadhesive polymers is one of the first efficient modification of conventional liquid ophthalmic formulations in order to prolong the residence time at ocular surface. The aim of this work was formulation of viscous ophthalmic vehicles/ocular lubricants containing derivative of guar gum, chitosan and cellulose derivatives (hypromellose and hydroxyethylcellulose) alone or in combination. Optimized formulations were developed by choosing appropriate polymer or combination of polymers in suitable concentrations with adequate physicochemical, rheological and mucoadhesive properties. Influence of the addition of simulated tear fluid, steam sterilization and storage on clarity, pH value and rheological properties of different formulations were evaluated. The compounded vehicles were compared with commercially available ocular lubricants containing similar polymers. It was observed that viscosities of commercially available eye drops were above the optimal viscosity range. The mucoadhesive potential of the vehicles, rheological synergism and type of polymermucin interaction were determined. Combination of chitosan and guar gum exhibited optimal physicochemical, rheological and mucoadhesive properties and had a potential for the longest retention time in tear film. Considering the simplicity of its preparation and stability (over a period of one-month storage), this vehicle could be used for extemporaneously compounded ocular preparations for specific needs of individual patients.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Drug Delivery Science and Technology",
title = "Development of polysaccharide-based mucoadhesive ophthalmic lubricating vehicles: The effect of different polymers on physicochemical properties and functionality",
volume = "49",
pages = "50-57",
doi = "10.1016/j.jddst.2018.10.034"
}

Račić, A., Čalija, B., Milić, J., Milasinvić, N.,& Krajišnik, D.. (2019). Development of polysaccharide-based mucoadhesive ophthalmic lubricating vehicles: The effect of different polymers on physicochemical properties and functionality. in Journal of Drug Delivery Science and Technology
Elsevier Science BV, Amsterdam., 49, 50-57.
https://doi.org/10.1016/j.jddst.2018.10.034

Račić A, Čalija B, Milić J, Milasinvić N, Krajišnik D. Development of polysaccharide-based mucoadhesive ophthalmic lubricating vehicles: The effect of different polymers on physicochemical properties and functionality. in Journal of Drug Delivery Science and Technology. 2019;49:50-57.
doi:10.1016/j.jddst.2018.10.034 .

Račić, Anđelka, Čalija, Bojan, Milić, Jela, Milasinvić, Nikola, Krajišnik, Danina, "Development of polysaccharide-based mucoadhesive ophthalmic lubricating vehicles: The effect of different polymers on physicochemical properties and functionality" in Journal of Drug Delivery Science and Technology, 49 (2019):50-57,
https://doi.org/10.1016/j.jddst.2018.10.034 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Čalija, Bojan
AU  - Micov, Ana
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3268
AB  - The physicochemical properties, stability, in vivo antihyperalgesic activity, and skin irritation potential of the carbomer hydrogels with the new chemical entity escin β-sitosterol (ES) phytosome were characterized and compared with those containing escin. Physicochemical characterization of the hydrogels (performed 48 hr after preparation) included organoleptic examination, pH measurement, light microscopy, differential scanning calorimetry analysis and rheological tests. The obtained results showed that increasing concentration of the active substances within 1–5% affected the appearance (color and transparency) of the hydrogels, their pH, consistency, and rheological behavior. Unlike acidic escin, which was dissolved in the liquid phase of the pseudoplastic hydrogels E1–E5 and reduced their maximal apparent viscosity (ηmax), minimal apparent viscosity (ηmin), and hysteresis area (H) in comparison to the plain carbomer hydrogel, amphiphilic ES-enhanced ηmax, ηmin, and thixotropy of the hydrogels ES1–ES5, which is favorable for prolonged retention at skin surface. Evaluation of in-use stability of the hydrogels showed that organoleptic characteristics, flow behavior, and pH values could be preserved for 3 months under ambient conditions. The rat ear test results suggested that the hydrogels are safe to be used on human skin. Both escin and ES-loaded hydrogels exerted significant, concentration-dependent antihyperalgesic effect in inflammatory pain model in rats. ES-loaded hydrogels were significantly more effective than those loaded with escin. This is a first report on the antihyperalgesic effect of topically applied escin as well as ES in a model of inflammatory pain.
PB  - Wiley-Liss Inc.
T2  - Drug Development Research
T1  - Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity
DO  - 10.1002/ddr.21572
ER  - 

@article{
author = "Đekić, Ljiljana and Čalija, Bojan and Micov, Ana and Tomić, Maja and Stepanović-Petrović, Radica",
year = "2019",
abstract = "The physicochemical properties, stability, in vivo antihyperalgesic activity, and skin irritation potential of the carbomer hydrogels with the new chemical entity escin β-sitosterol (ES) phytosome were characterized and compared with those containing escin. Physicochemical characterization of the hydrogels (performed 48 hr after preparation) included organoleptic examination, pH measurement, light microscopy, differential scanning calorimetry analysis and rheological tests. The obtained results showed that increasing concentration of the active substances within 1–5% affected the appearance (color and transparency) of the hydrogels, their pH, consistency, and rheological behavior. Unlike acidic escin, which was dissolved in the liquid phase of the pseudoplastic hydrogels E1–E5 and reduced their maximal apparent viscosity (ηmax), minimal apparent viscosity (ηmin), and hysteresis area (H) in comparison to the plain carbomer hydrogel, amphiphilic ES-enhanced ηmax, ηmin, and thixotropy of the hydrogels ES1–ES5, which is favorable for prolonged retention at skin surface. Evaluation of in-use stability of the hydrogels showed that organoleptic characteristics, flow behavior, and pH values could be preserved for 3 months under ambient conditions. The rat ear test results suggested that the hydrogels are safe to be used on human skin. Both escin and ES-loaded hydrogels exerted significant, concentration-dependent antihyperalgesic effect in inflammatory pain model in rats. ES-loaded hydrogels were significantly more effective than those loaded with escin. This is a first report on the antihyperalgesic effect of topically applied escin as well as ES in a model of inflammatory pain.",
publisher = "Wiley-Liss Inc.",
journal = "Drug Development Research",
title = "Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity",
doi = "10.1002/ddr.21572"
}

Đekić, L., Čalija, B., Micov, A., Tomić, M.,& Stepanović-Petrović, R.. (2019). Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity. in Drug Development Research
Wiley-Liss Inc...
https://doi.org/10.1002/ddr.21572

Đekić L, Čalija B, Micov A, Tomić M, Stepanović-Petrović R. Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity. in Drug Development Research. 2019;.
doi:10.1002/ddr.21572 .

Đekić, Ljiljana, Čalija, Bojan, Micov, Ana, Tomić, Maja, Stepanović-Petrović, Radica, "Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity" in Drug Development Research (2019),
https://doi.org/10.1002/ddr.21572 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Martinović, Martina
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Medarević, Đorđe
AU  - Primorac, Marija
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3305
AB  - The study is focused on formulation of biocompatible hydrogels with a poorly soluble drug ibuprofen (5%) and comprehensive evaluation and comparison of effect of different bioadhesive polymers on their suitability for application on skin, physical stability during the accelerated and natural aging tests (by performing centrifugation test, light microscopy, differential scanning calorimetry, rheological and pH measurements), and in vitro drug release kinetics. Hydrogels, formulated with xanthan gum 1% (XIB), sodium carboxymethylcellulose 5% (CMCIB), poloxamer 407 16% (PIB), and carbomer 1% (KIB), were soft pseudoplastic semisolids with thixotropy and biocompatible pH. The type of the polymer significantly affected apparent viscosity of the hydrogels and miscibility rate with artificial sweat, their physical stability, and shape, size, and aggregation of the drug crystals and degree of crystallization. The drug release in all investigated hydrogels was diffusion-controlled in accordance with the Higuchi model and sustained for 12 h, with the drug release rate and the amount of drug released depended on the polymer. The described formulation approach enabled discrimination of the hydrogels with unsatisfactory application properties (CMCIB) and physical stability (KIB), and selection of the hydrogel with promising characteristics in terms of all investigated aspects (XIB) which could be considered for further evaluation.
PB  - Elsevier Science Inc, New York
T2  - Journal of Pharmaceutical Sciences
T1  - Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen
VL  - 108
IS  - 3
SP  - 1326
EP  - 1333
DO  - 10.1016/j.xphs.2018.10.054
ER  - 

@article{
author = "Đekić, Ljiljana and Martinović, Martina and Dobričić, Vladimir and Čalija, Bojan and Medarević, Đorđe and Primorac, Marija",
year = "2019",
abstract = "The study is focused on formulation of biocompatible hydrogels with a poorly soluble drug ibuprofen (5%) and comprehensive evaluation and comparison of effect of different bioadhesive polymers on their suitability for application on skin, physical stability during the accelerated and natural aging tests (by performing centrifugation test, light microscopy, differential scanning calorimetry, rheological and pH measurements), and in vitro drug release kinetics. Hydrogels, formulated with xanthan gum 1% (XIB), sodium carboxymethylcellulose 5% (CMCIB), poloxamer 407 16% (PIB), and carbomer 1% (KIB), were soft pseudoplastic semisolids with thixotropy and biocompatible pH. The type of the polymer significantly affected apparent viscosity of the hydrogels and miscibility rate with artificial sweat, their physical stability, and shape, size, and aggregation of the drug crystals and degree of crystallization. The drug release in all investigated hydrogels was diffusion-controlled in accordance with the Higuchi model and sustained for 12 h, with the drug release rate and the amount of drug released depended on the polymer. The described formulation approach enabled discrimination of the hydrogels with unsatisfactory application properties (CMCIB) and physical stability (KIB), and selection of the hydrogel with promising characteristics in terms of all investigated aspects (XIB) which could be considered for further evaluation.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Pharmaceutical Sciences",
title = "Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen",
volume = "108",
number = "3",
pages = "1326-1333",
doi = "10.1016/j.xphs.2018.10.054"
}

Đekić, L., Martinović, M., Dobričić, V., Čalija, B., Medarević, Đ.,& Primorac, M.. (2019). Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen. in Journal of Pharmaceutical Sciences
Elsevier Science Inc, New York., 108(3), 1326-1333.
https://doi.org/10.1016/j.xphs.2018.10.054

Đekić L, Martinović M, Dobričić V, Čalija B, Medarević Đ, Primorac M. Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen. in Journal of Pharmaceutical Sciences. 2019;108(3):1326-1333.
doi:10.1016/j.xphs.2018.10.054 .

Đekić, Ljiljana, Martinović, Martina, Dobričić, Vladimir, Čalija, Bojan, Medarević, Đorđe, Primorac, Marija, "Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen" in Journal of Pharmaceutical Sciences, 108, no. 3 (2019):1326-1333,
https://doi.org/10.1016/j.xphs.2018.10.054 . .

TY  - JOUR
AU  - Bubić-Pajić, Nataša
AU  - Nikolić, Ines
AU  - Mitsou, Evgenia
AU  - Papadimitriou, Vassiliki
AU  - Xenakis, Aristotelis
AU  - Ranđelović, Danijela
AU  - Dobričić, Vladimir
AU  - Smitran, Aleksandra
AU  - Cekić, Nebojša
AU  - Čalija, Bojan
AU  - Savić, Snežana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3062
AB  - The aim of this study was development of biocompatible topical microemulsions (MEs) for incorporation and improved dermal delivery of sertaconazole nitrate (SN). For this purpose, phase behavior and microstructure of pseudo-ternary glycereth-7-caprylate/caprate (Emanon EV-E, EV)/cosurfactant/Capryol (TM) 90/water systems were investigated. Furhermore, the influence of these properties on the drug skin delivery was also assessed. Expansion of ME single-phase regions with the use of short chain alcohols was a consequence of the more fluid interface when compared to other investigated systems, which was confirmed by electron paramagnetic resonance spectroscopy-EPR. The chosen bicontinuous to inverted bicontinuous formulations were assessed against the ME based on polysorbate 80 as referent sample. Despite incorporation of SN within the selected formulations induced similar alternations in electrical conductivity, viscosity and pH values, obtained EPR spectra suggested different SN localization: within the oil phase (for most of the EV based formulations), or interacting with the interface (polysorbate 80 based formulation). Due to higher in vitro drug release (12.24%-18.53%), ex vivo SN penetration into porcine ear skin (dermal retention Enhancement Ratio (ERO) ranged from 2.66 to 4.25) and pronounced antifungal activity, the chosen MEs represent promising vehicles for dermal delivery of SN in treatment of cutaneous fungal infections. The biopharmaceutical and skin performance differences obtained with different formulations were possible to be explained on the basis of their physicochemical characteristics.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Molecular Liquids
T1  - Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: Phase behavior study and microstructure influence on drug biopharamaceutical properties
VL  - 272
SP  - 746
EP  - 758
DO  - 10.1016/j.molliq.2018.10.002
ER  - 

@article{
author = "Bubić-Pajić, Nataša and Nikolić, Ines and Mitsou, Evgenia and Papadimitriou, Vassiliki and Xenakis, Aristotelis and Ranđelović, Danijela and Dobričić, Vladimir and Smitran, Aleksandra and Cekić, Nebojša and Čalija, Bojan and Savić, Snežana",
year = "2018",
abstract = "The aim of this study was development of biocompatible topical microemulsions (MEs) for incorporation and improved dermal delivery of sertaconazole nitrate (SN). For this purpose, phase behavior and microstructure of pseudo-ternary glycereth-7-caprylate/caprate (Emanon EV-E, EV)/cosurfactant/Capryol (TM) 90/water systems were investigated. Furhermore, the influence of these properties on the drug skin delivery was also assessed. Expansion of ME single-phase regions with the use of short chain alcohols was a consequence of the more fluid interface when compared to other investigated systems, which was confirmed by electron paramagnetic resonance spectroscopy-EPR. The chosen bicontinuous to inverted bicontinuous formulations were assessed against the ME based on polysorbate 80 as referent sample. Despite incorporation of SN within the selected formulations induced similar alternations in electrical conductivity, viscosity and pH values, obtained EPR spectra suggested different SN localization: within the oil phase (for most of the EV based formulations), or interacting with the interface (polysorbate 80 based formulation). Due to higher in vitro drug release (12.24%-18.53%), ex vivo SN penetration into porcine ear skin (dermal retention Enhancement Ratio (ERO) ranged from 2.66 to 4.25) and pronounced antifungal activity, the chosen MEs represent promising vehicles for dermal delivery of SN in treatment of cutaneous fungal infections. The biopharmaceutical and skin performance differences obtained with different formulations were possible to be explained on the basis of their physicochemical characteristics.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Molecular Liquids",
title = "Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: Phase behavior study and microstructure influence on drug biopharamaceutical properties",
volume = "272",
pages = "746-758",
doi = "10.1016/j.molliq.2018.10.002"
}

Bubić-Pajić, N., Nikolić, I., Mitsou, E., Papadimitriou, V., Xenakis, A., Ranđelović, D., Dobričić, V., Smitran, A., Cekić, N., Čalija, B.,& Savić, S.. (2018). Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: Phase behavior study and microstructure influence on drug biopharamaceutical properties. in Journal of Molecular Liquids
Elsevier Science BV, Amsterdam., 272, 746-758.
https://doi.org/10.1016/j.molliq.2018.10.002

Bubić-Pajić N, Nikolić I, Mitsou E, Papadimitriou V, Xenakis A, Ranđelović D, Dobričić V, Smitran A, Cekić N, Čalija B, Savić S. Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: Phase behavior study and microstructure influence on drug biopharamaceutical properties. in Journal of Molecular Liquids. 2018;272:746-758.
doi:10.1016/j.molliq.2018.10.002 .

Bubić-Pajić, Nataša, Nikolić, Ines, Mitsou, Evgenia, Papadimitriou, Vassiliki, Xenakis, Aristotelis, Ranđelović, Danijela, Dobričić, Vladimir, Smitran, Aleksandra, Cekić, Nebojša, Čalija, Bojan, Savić, Snežana, "Biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate: Phase behavior study and microstructure influence on drug biopharamaceutical properties" in Journal of Molecular Liquids, 272 (2018):746-758,
https://doi.org/10.1016/j.molliq.2018.10.002 . .

TY  - JOUR
AU  - Janićijević, Jelena
AU  - Milić, Jela
AU  - Čalija, Bojan
AU  - Micov, Ana
AU  - Stepanović-Petrović, Radica
AU  - Tomić, Maja
AU  - Daković, Aleksandra
AU  - Dobričić, Vladimir
AU  - Nedić-Vasiljević, Bojana
AU  - Krajišnik, Danina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3050
AB  - Refined diatomite from the Kolubara coal basin (Serbia) was inorganically functionalized through a simple, one-pot, non-time-consuming procedure. Model drug ibuprofen was adsorbed on the functionalized diatomite under optimized conditions providing high drug Loading (similar to 201 mg g(-1)). Physicochemical characterization was performed on the starting and modified diatomite before and after ibuprofen adsorption. Dissolution testing was conducted on comprimates containing the drug adsorbed on the modified diatomite (composite) and those containing a physical mixture of the drug with the modified diatomite. The antihyperalgesic and the antiedematous activity of ibuprofen from both composites and physical mixtures were evaluated in vivo employing an inflammatory pain model in rats. Functionalization and subsequent drug adsorption had no significant effect on the diatomite ordered porous structure. Two forms of ibuprofen most likely coexisted in the adsorbed state - the acidic form and a salt/complex with aluminium. Both comprimate types showed extended ibuprofen release in vitro, but no significant influence on the duration of the ibuprofen effect was observed upon in vivo application of the composite or physical mixture. However, both the composite and the physical mixture were more effective than equivalent doses of ibuprofen in pain suppression in rats. This potentiation of the ibuprofen antihyperalgesic effect may result from the formation of the drug complex with the carrier and can be of clinical relevance.
PB  - Royal Soc Chemistry, Cambridge
T2  - Journal of Materials Chemistry B
T1  - Potentiation of the ibuprofen antihyperalgesic effect using inorganically functionalized diatomite
VL  - 6
IS  - 36
SP  - 5812
EP  - 5822
DO  - 10.1039/c8tb01376d
ER  - 

@article{
author = "Janićijević, Jelena and Milić, Jela and Čalija, Bojan and Micov, Ana and Stepanović-Petrović, Radica and Tomić, Maja and Daković, Aleksandra and Dobričić, Vladimir and Nedić-Vasiljević, Bojana and Krajišnik, Danina",
year = "2018",
abstract = "Refined diatomite from the Kolubara coal basin (Serbia) was inorganically functionalized through a simple, one-pot, non-time-consuming procedure. Model drug ibuprofen was adsorbed on the functionalized diatomite under optimized conditions providing high drug Loading (similar to 201 mg g(-1)). Physicochemical characterization was performed on the starting and modified diatomite before and after ibuprofen adsorption. Dissolution testing was conducted on comprimates containing the drug adsorbed on the modified diatomite (composite) and those containing a physical mixture of the drug with the modified diatomite. The antihyperalgesic and the antiedematous activity of ibuprofen from both composites and physical mixtures were evaluated in vivo employing an inflammatory pain model in rats. Functionalization and subsequent drug adsorption had no significant effect on the diatomite ordered porous structure. Two forms of ibuprofen most likely coexisted in the adsorbed state - the acidic form and a salt/complex with aluminium. Both comprimate types showed extended ibuprofen release in vitro, but no significant influence on the duration of the ibuprofen effect was observed upon in vivo application of the composite or physical mixture. However, both the composite and the physical mixture were more effective than equivalent doses of ibuprofen in pain suppression in rats. This potentiation of the ibuprofen antihyperalgesic effect may result from the formation of the drug complex with the carrier and can be of clinical relevance.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Journal of Materials Chemistry B",
title = "Potentiation of the ibuprofen antihyperalgesic effect using inorganically functionalized diatomite",
volume = "6",
number = "36",
pages = "5812-5822",
doi = "10.1039/c8tb01376d"
}

Janićijević, J., Milić, J., Čalija, B., Micov, A., Stepanović-Petrović, R., Tomić, M., Daković, A., Dobričić, V., Nedić-Vasiljević, B.,& Krajišnik, D.. (2018). Potentiation of the ibuprofen antihyperalgesic effect using inorganically functionalized diatomite. in Journal of Materials Chemistry B
Royal Soc Chemistry, Cambridge., 6(36), 5812-5822.
https://doi.org/10.1039/c8tb01376d

Janićijević J, Milić J, Čalija B, Micov A, Stepanović-Petrović R, Tomić M, Daković A, Dobričić V, Nedić-Vasiljević B, Krajišnik D. Potentiation of the ibuprofen antihyperalgesic effect using inorganically functionalized diatomite. in Journal of Materials Chemistry B. 2018;6(36):5812-5822.
doi:10.1039/c8tb01376d .

Janićijević, Jelena, Milić, Jela, Čalija, Bojan, Micov, Ana, Stepanović-Petrović, Radica, Tomić, Maja, Daković, Aleksandra, Dobričić, Vladimir, Nedić-Vasiljević, Bojana, Krajišnik, Danina, "Potentiation of the ibuprofen antihyperalgesic effect using inorganically functionalized diatomite" in Journal of Materials Chemistry B, 6, no. 36 (2018):5812-5822,
https://doi.org/10.1039/c8tb01376d . .

TY  - JOUR
AU  - Bubić-Pajić, Nataša
AU  - Todosijević, Marija N.
AU  - Vuleta, Gordana
AU  - Cekić, Nebojša
AU  - Dobričić, Vladimir
AU  - Vučen, Sonja
AU  - Čalija, Bojan
AU  - Lukić, Milica
AU  - Ilić, Tanja
AU  - Savić, Snežana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2837
AB  - Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth-7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazole nitrate incorporation, while they remained almost unchanged in adapalene-loaded vehicles. In addition, results of the in vivo skin performance study supported acceptable tolerability for all investigated formulations, suggesting selected microemulsions as promising carriers worth exploring further for effective skin delivery of model drugs.
PB  - Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb
T2  - Acta Pharmaceutica
T1  - Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance
VL  - 67
IS  - 4
SP  - 415
EP  - 439
DO  - 10.1515/acph-2017-0036
ER  - 

@article{
author = "Bubić-Pajić, Nataša and Todosijević, Marija N. and Vuleta, Gordana and Cekić, Nebojša and Dobričić, Vladimir and Vučen, Sonja and Čalija, Bojan and Lukić, Milica and Ilić, Tanja and Savić, Snežana",
year = "2017",
abstract = "Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth-7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazole nitrate incorporation, while they remained almost unchanged in adapalene-loaded vehicles. In addition, results of the in vivo skin performance study supported acceptable tolerability for all investigated formulations, suggesting selected microemulsions as promising carriers worth exploring further for effective skin delivery of model drugs.",
publisher = "Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb",
journal = "Acta Pharmaceutica",
title = "Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance",
volume = "67",
number = "4",
pages = "415-439",
doi = "10.1515/acph-2017-0036"
}

Bubić-Pajić, N., Todosijević, M. N., Vuleta, G., Cekić, N., Dobričić, V., Vučen, S., Čalija, B., Lukić, M., Ilić, T.,& Savić, S.. (2017). Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance. in Acta Pharmaceutica
Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb., 67(4), 415-439.
https://doi.org/10.1515/acph-2017-0036

Bubić-Pajić N, Todosijević MN, Vuleta G, Cekić N, Dobričić V, Vučen S, Čalija B, Lukić M, Ilić T, Savić S. Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance. in Acta Pharmaceutica. 2017;67(4):415-439.
doi:10.1515/acph-2017-0036 .

Bubić-Pajić, Nataša, Todosijević, Marija N., Vuleta, Gordana, Cekić, Nebojša, Dobričić, Vladimir, Vučen, Sonja, Čalija, Bojan, Lukić, Milica, Ilić, Tanja, Savić, Snežana, "Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance" in Acta Pharmaceutica, 67, no. 4 (2017):415-439,
https://doi.org/10.1515/acph-2017-0036 . .