TY  - JOUR
AU  - Brborić, Jasmina
AU  - Klisić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Delogu, Giovanna
AU  - Gjorgieva Ackova, Darinka
AU  - Kostić, Kristina
AU  - Dettori, Maria Antonietta
AU  - Fabbri, Davide
AU  - Carta, Paola
AU  - Saso, Luciano
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4964
AB  - Introduction: Phenols are a large family of natural and synthetic compounds with known antioxidant activity. The aim of this study was to perform in vitro screening of natural and natural-like phenol monomers and their C2-symmetric dimers (hydroxylated biphenyls) in order to identify those representatives whose pharmacophores have the strongest antioxidant and the lowest prooxidant activity. Material and methods: Antioxidative properties of 36 compounds (monomers and their C2-symmetric dimers) were evaluated in vitro. Different (red/ ox) assays were used to measure their total oxidative potential (TOP), their total antioxidative capacity (TAC), the pro-oxidative-antioxidant balance (PAB) and total SH-group content (SHG) in a biologically relevant environment. The Pro-oxidative Score, Antioxidative Score and the Oxy Score were also calculated. Trolox, a water soluble analogue of α-tocopherol, was used as a positive control. Results: In an assay consisting of pooled human serum, 6 of the 36 compounds showed significant antioxidant activity (compounds 6, 7, 12, 13, 26, and 27), whereas 4 showed extremely weak antioxidant activity (compounds 2, 29, 30, and 31). Within the 36 compounds comprising zingerone, dehydrozingerone, aurone, chalcone, and magnolol derivatives, in both monomeric and dimeric forms, the 2 compounds that indicated the highest antioxidant activity were dehydrozingerone derivatives (compounds 6 and 12). Trolox’s activity was found between the strong and weak antioxidant compounds analysed in our study. Conclusions: In this study selected dehydrozingerones were identified as good candidates for in-depth testing of their biological behaviour and for possible precursors for synthesis of novel polyphenolic molecules with potential therapeutic applications.
PB  - Termedia Publishing House
T2  - Archives of Medical Science
T1  - Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application
VL  - 19
IS  - 3
SP  - 651
EP  - 671
DO  - 10.5114/aoms/135379
ER  - 

@article{
author = "Brborić, Jasmina and Klisić, Aleksandra and Kotur-Stevuljević, Jelena and Delogu, Giovanna and Gjorgieva Ackova, Darinka and Kostić, Kristina and Dettori, Maria Antonietta and Fabbri, Davide and Carta, Paola and Saso, Luciano",
year = "2023",
abstract = "Introduction: Phenols are a large family of natural and synthetic compounds with known antioxidant activity. The aim of this study was to perform in vitro screening of natural and natural-like phenol monomers and their C2-symmetric dimers (hydroxylated biphenyls) in order to identify those representatives whose pharmacophores have the strongest antioxidant and the lowest prooxidant activity. Material and methods: Antioxidative properties of 36 compounds (monomers and their C2-symmetric dimers) were evaluated in vitro. Different (red/ ox) assays were used to measure their total oxidative potential (TOP), their total antioxidative capacity (TAC), the pro-oxidative-antioxidant balance (PAB) and total SH-group content (SHG) in a biologically relevant environment. The Pro-oxidative Score, Antioxidative Score and the Oxy Score were also calculated. Trolox, a water soluble analogue of α-tocopherol, was used as a positive control. Results: In an assay consisting of pooled human serum, 6 of the 36 compounds showed significant antioxidant activity (compounds 6, 7, 12, 13, 26, and 27), whereas 4 showed extremely weak antioxidant activity (compounds 2, 29, 30, and 31). Within the 36 compounds comprising zingerone, dehydrozingerone, aurone, chalcone, and magnolol derivatives, in both monomeric and dimeric forms, the 2 compounds that indicated the highest antioxidant activity were dehydrozingerone derivatives (compounds 6 and 12). Trolox’s activity was found between the strong and weak antioxidant compounds analysed in our study. Conclusions: In this study selected dehydrozingerones were identified as good candidates for in-depth testing of their biological behaviour and for possible precursors for synthesis of novel polyphenolic molecules with potential therapeutic applications.",
publisher = "Termedia Publishing House",
journal = "Archives of Medical Science",
title = "Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application",
volume = "19",
number = "3",
pages = "651-671",
doi = "10.5114/aoms/135379"
}

Brborić, J., Klisić, A., Kotur-Stevuljević, J., Delogu, G., Gjorgieva Ackova, D., Kostić, K., Dettori, M. A., Fabbri, D., Carta, P.,& Saso, L.. (2023). Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application. in Archives of Medical Science
Termedia Publishing House., 19(3), 651-671.
https://doi.org/10.5114/aoms/135379

Brborić J, Klisić A, Kotur-Stevuljević J, Delogu G, Gjorgieva Ackova D, Kostić K, Dettori MA, Fabbri D, Carta P, Saso L. Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application. in Archives of Medical Science. 2023;19(3):651-671.
doi:10.5114/aoms/135379 .

Brborić, Jasmina, Klisić, Aleksandra, Kotur-Stevuljević, Jelena, Delogu, Giovanna, Gjorgieva Ackova, Darinka, Kostić, Kristina, Dettori, Maria Antonietta, Fabbri, Davide, Carta, Paola, Saso, Luciano, "Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application" in Archives of Medical Science, 19, no. 3 (2023):651-671,
https://doi.org/10.5114/aoms/135379 . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Antonijević, Marija
AU  - Crevar, Milkica
AU  - Brborić, Jasmina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4978
AB  - Whereas nonselective nonsteroidal anti-inflammatory drugs, such as aspirin, ibuprofen and
diclofenac, inhibit both cyclooxygenase-1 and cyclooxigenase-2 enzymes, selective inhibitors
target cyclooxygenase-2, which is overexpressed in inflammation, but also in cancer,
atherosclerosis, Alzheimer's disease, and Parkinson`s disease. Potential cardiovascular and
hepatic side effects of cyclooxygenase-2 inhibitors have limited their use. The development of
selective and safe cyclooxygenase-2 inhibitors remains a high priority in drug discovery. Based
on the structure of previously investigated newly synthesized β-hydroxy-β-arylpropanoic acids,
two groups of compounds were designed: analogs in which one of the benzene rings was replaced
by a pyrazole, while the carboxyl group was retained, and amides of β-hydroxy-β-arylpropanoic
acids with pyrazole. The compounds were docked into the 3D structure of the catalytic site of the
enzyme cyclooxygenase-2 using AutoDock Vina 1.2.0. and the obtained interactions were
compared with the interactions of celecoxib, a selective inhibitor. The amides had lower binding
energies than the designed acids, which makes them attractive target compounds for synthesis
and further examination.
AB  - Neselektivni nesteroidni antiinflamatorni lekovi poput aspirina, ibuprofena i diklofenaka
inhibiraju enzime ciklooksigenazu-1 i ciklooksigenazu-2, a selektivni inhibitori ciljaju
ciklooksigenazu-2 koja je prekomerno izražena u inflamaciji, ali takođe i kod kancera,
ateroskleroze, Parkinsonove i Alchajmerove bolesti. Potencijalni kardiovaskularni i hepatički
neželjeni efekti selektivnih inhibitora ciklooksigenaze-2 su ograničili njihovu primenu. Razvoj
selektivnih i bezbednih inhibitora ciklooksigenaze-2 ostaje veoma prioritetna oblast u otkrivanju
lekova. Na osnovu strukture prethodno istraživanih novosintetisanih β-hidroksi-β-arilpropanskih
kiselina dizajnirane su dve grupe jedinjenja: analozi u kojima je jedan od benzenovih prstenova
zamenjen pirazolom, uz zadržavanje karboksilne grupe, i amidi β-hidroksi-β-arilpropanskih
kiselina sa pirazolom. Program AutoDock Vina 1.2.0 je korišćen za dokovanje dizajniranih
jedinjenja u 3D strukturu katalitičkog mesta enzima ciklooksigenaze-2, a ostvarene interakcije su
upoređene sa interakcijama koje ostvaruje selektivni inhibitor celekoksib. Amidi su imali nižu
energiju vezivanja od kiselina, što ih čini dobrim kandidatima za sintezu.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
T2  - Arhiv za farmaciju
T1  - Docking studies of some pyrazole containing compounds in the cyclooxygenase-2 active site
T1  - Doking studije nekih jedinjenja sa pirazolom u aktivnom mestu ciklooksigenaze-2
VL  - 73
IS  - 3
SP  - 205
EP  - 215
DO  - 10.5937/arhfarm73-44720
ER  - 

@article{
author = "Savić, Jelena and Antonijević, Marija and Crevar, Milkica and Brborić, Jasmina",
year = "2023",
abstract = "Whereas nonselective nonsteroidal anti-inflammatory drugs, such as aspirin, ibuprofen and
diclofenac, inhibit both cyclooxygenase-1 and cyclooxigenase-2 enzymes, selective inhibitors
target cyclooxygenase-2, which is overexpressed in inflammation, but also in cancer,
atherosclerosis, Alzheimer's disease, and Parkinson`s disease. Potential cardiovascular and
hepatic side effects of cyclooxygenase-2 inhibitors have limited their use. The development of
selective and safe cyclooxygenase-2 inhibitors remains a high priority in drug discovery. Based
on the structure of previously investigated newly synthesized β-hydroxy-β-arylpropanoic acids,
two groups of compounds were designed: analogs in which one of the benzene rings was replaced
by a pyrazole, while the carboxyl group was retained, and amides of β-hydroxy-β-arylpropanoic
acids with pyrazole. The compounds were docked into the 3D structure of the catalytic site of the
enzyme cyclooxygenase-2 using AutoDock Vina 1.2.0. and the obtained interactions were
compared with the interactions of celecoxib, a selective inhibitor. The amides had lower binding
energies than the designed acids, which makes them attractive target compounds for synthesis
and further examination., Neselektivni nesteroidni antiinflamatorni lekovi poput aspirina, ibuprofena i diklofenaka
inhibiraju enzime ciklooksigenazu-1 i ciklooksigenazu-2, a selektivni inhibitori ciljaju
ciklooksigenazu-2 koja je prekomerno izražena u inflamaciji, ali takođe i kod kancera,
ateroskleroze, Parkinsonove i Alchajmerove bolesti. Potencijalni kardiovaskularni i hepatički
neželjeni efekti selektivnih inhibitora ciklooksigenaze-2 su ograničili njihovu primenu. Razvoj
selektivnih i bezbednih inhibitora ciklooksigenaze-2 ostaje veoma prioritetna oblast u otkrivanju
lekova. Na osnovu strukture prethodno istraživanih novosintetisanih β-hidroksi-β-arilpropanskih
kiselina dizajnirane su dve grupe jedinjenja: analozi u kojima je jedan od benzenovih prstenova
zamenjen pirazolom, uz zadržavanje karboksilne grupe, i amidi β-hidroksi-β-arilpropanskih
kiselina sa pirazolom. Program AutoDock Vina 1.2.0 je korišćen za dokovanje dizajniranih
jedinjenja u 3D strukturu katalitičkog mesta enzima ciklooksigenaze-2, a ostvarene interakcije su
upoređene sa interakcijama koje ostvaruje selektivni inhibitor celekoksib. Amidi su imali nižu
energiju vezivanja od kiselina, što ih čini dobrim kandidatima za sintezu.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Docking studies of some pyrazole containing compounds in the cyclooxygenase-2 active site, Doking studije nekih jedinjenja sa pirazolom u aktivnom mestu ciklooksigenaze-2",
volume = "73",
number = "3",
pages = "205-215",
doi = "10.5937/arhfarm73-44720"
}

Savić, J., Antonijević, M., Crevar, M.,& Brborić, J.. (2023). Docking studies of some pyrazole containing compounds in the cyclooxygenase-2 active site. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 73(3), 205-215.
https://doi.org/10.5937/arhfarm73-44720

Savić J, Antonijević M, Crevar M, Brborić J. Docking studies of some pyrazole containing compounds in the cyclooxygenase-2 active site. in Arhiv za farmaciju. 2023;73(3):205-215.
doi:10.5937/arhfarm73-44720 .

Savić, Jelena, Antonijević, Marija, Crevar, Milkica, Brborić, Jasmina, "Docking studies of some pyrazole containing compounds in the cyclooxygenase-2 active site" in Arhiv za farmaciju, 73, no. 3 (2023):205-215,
https://doi.org/10.5937/arhfarm73-44720 . .

TY  - JOUR
AU  - Rupar, Jelena
AU  - Dobričić, Vladimir
AU  - Brborić, Jasmina
AU  - Čudina, Olivera
AU  - Aleksić, Mara
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4323
AB  - Electrochemical oxidation of newly synthesized acridine derivatives (ADs): PG, PA, EG and EA was studied using square wave voltammetry at a glassy carbon electrode. Oxidation occurs as an irreversible process for all ADs. The ADs interaction with DNA was investigated using multi-layer DNA electrochemical biosensor. The shift of dA peak in positive direction indicated that the type of interaction is most likely an intercalation. PG showed the widest range of concentration capable to interact with DNA (1 × 10−7 M − 2.5 × 10−4 M). Analysing [Formula presented] vs logcAD plots, the binding constants were determined. For the lowest PG concentrations, obtained K value close to 106 M−1 refers to strong binding. The values of K for PA may be classified as medium strength, while EG and EA showed low K values. Our results unequivocally showed that the characteristics of association complexes may vary depending on the concentration of the interacting substance. The negative ΔG value for all ADs, (- 21 to - 34 kJ mol−1), confirms the process spontaneity. The best result, indicating the most stable formed complex with DNA adsorbed at the electrode surface, showed PG when present in low concentration, order of 10−7 M. The intercalation of ADs into DNA was supported by molecular docking analysis.
PB  - Elsevier B.V.
T2  - Bioelectrochemistry
T1  - Square wave voltammetric study of interaction between 9-acridinyl amino acid derivatives and DNA
VL  - 149
DO  - 10.1016/j.bioelechem.2022.108323
ER  - 

@article{
author = "Rupar, Jelena and Dobričić, Vladimir and Brborić, Jasmina and Čudina, Olivera and Aleksić, Mara",
year = "2023",
abstract = "Electrochemical oxidation of newly synthesized acridine derivatives (ADs): PG, PA, EG and EA was studied using square wave voltammetry at a glassy carbon electrode. Oxidation occurs as an irreversible process for all ADs. The ADs interaction with DNA was investigated using multi-layer DNA electrochemical biosensor. The shift of dA peak in positive direction indicated that the type of interaction is most likely an intercalation. PG showed the widest range of concentration capable to interact with DNA (1 × 10−7 M − 2.5 × 10−4 M). Analysing [Formula presented] vs logcAD plots, the binding constants were determined. For the lowest PG concentrations, obtained K value close to 106 M−1 refers to strong binding. The values of K for PA may be classified as medium strength, while EG and EA showed low K values. Our results unequivocally showed that the characteristics of association complexes may vary depending on the concentration of the interacting substance. The negative ΔG value for all ADs, (- 21 to - 34 kJ mol−1), confirms the process spontaneity. The best result, indicating the most stable formed complex with DNA adsorbed at the electrode surface, showed PG when present in low concentration, order of 10−7 M. The intercalation of ADs into DNA was supported by molecular docking analysis.",
publisher = "Elsevier B.V.",
journal = "Bioelectrochemistry",
title = "Square wave voltammetric study of interaction between 9-acridinyl amino acid derivatives and DNA",
volume = "149",
doi = "10.1016/j.bioelechem.2022.108323"
}

Rupar, J., Dobričić, V., Brborić, J., Čudina, O.,& Aleksić, M.. (2023). Square wave voltammetric study of interaction between 9-acridinyl amino acid derivatives and DNA. in Bioelectrochemistry
Elsevier B.V.., 149.
https://doi.org/10.1016/j.bioelechem.2022.108323

Rupar J, Dobričić V, Brborić J, Čudina O, Aleksić M. Square wave voltammetric study of interaction between 9-acridinyl amino acid derivatives and DNA. in Bioelectrochemistry. 2023;149.
doi:10.1016/j.bioelechem.2022.108323 .

Rupar, Jelena, Dobričić, Vladimir, Brborić, Jasmina, Čudina, Olivera, Aleksić, Mara, "Square wave voltammetric study of interaction between 9-acridinyl amino acid derivatives and DNA" in Bioelectrochemistry, 149 (2023),
https://doi.org/10.1016/j.bioelechem.2022.108323 . .

TY  - JOUR
AU  - Kostić, Kristina
AU  - Brborić, Jasmina
AU  - Delogu, Giovanna
AU  - Simić, Milena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
AU  - Dettori, Maria Antonietta
AU  - Fabbri, Davide
AU  - Kotur-Stevuljević, Jelena
AU  - Saso, Luciano
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4648
AB  - A comparative in vitro study of the antioxidant potential of natural phenols (zingerone, curcumin, raspberry ketone, magnolol) and their synthesized derivatives was performed. The antioxidant efficiency was evaluated in blood serum obtained from healthy individuals, by means of spectrophotometry, before and after the addition of pro-oxidant tert-butyl hydroperoxide (TBH). Moreover, the antioxidant effect of an equimolar mixture of curcumin and zingerone was investigated. Interpretation of our results reveals that in the blood serum of healthy individuals curcumin (C1), raspberry ketone (RK1), magnolol (M1) and synthesized derivative of zingerone (Z2) demonstrate remarkable antioxidant effects (p < 0.05). However, in the state of TBH-induced excessive oxidative stress natural magnolol and synthesized derivatives C1, Z1 and RK1 show powerful antioxidant activity and thus can be further investigated to obtain information about their metabolic transformations and their potential influence at the cellular level. Results obtained from measurements in an equimolar mixture of zingerone and curcumin indicate synergism (p < 0.05) between the two compounds. This combination is especially successful due to the fast and efficient neutralization of added pro-oxidant TBH. The commercial availability of turmeric and ginger and their frequent combined use in diet suggest ideas for further broader utilization of the beneficial synergistic effect of their phenolic components.
PB  - MDPI
T2  - Molecules
T1  - Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls
VL  - 28
IS  - 6
DO  - 10.3390/molecules28062646
ER  - 

@article{
author = "Kostić, Kristina and Brborić, Jasmina and Delogu, Giovanna and Simić, Milena and Samardžić, Stevan and Maksimović, Zoran and Dettori, Maria Antonietta and Fabbri, Davide and Kotur-Stevuljević, Jelena and Saso, Luciano",
year = "2023",
abstract = "A comparative in vitro study of the antioxidant potential of natural phenols (zingerone, curcumin, raspberry ketone, magnolol) and their synthesized derivatives was performed. The antioxidant efficiency was evaluated in blood serum obtained from healthy individuals, by means of spectrophotometry, before and after the addition of pro-oxidant tert-butyl hydroperoxide (TBH). Moreover, the antioxidant effect of an equimolar mixture of curcumin and zingerone was investigated. Interpretation of our results reveals that in the blood serum of healthy individuals curcumin (C1), raspberry ketone (RK1), magnolol (M1) and synthesized derivative of zingerone (Z2) demonstrate remarkable antioxidant effects (p < 0.05). However, in the state of TBH-induced excessive oxidative stress natural magnolol and synthesized derivatives C1, Z1 and RK1 show powerful antioxidant activity and thus can be further investigated to obtain information about their metabolic transformations and their potential influence at the cellular level. Results obtained from measurements in an equimolar mixture of zingerone and curcumin indicate synergism (p < 0.05) between the two compounds. This combination is especially successful due to the fast and efficient neutralization of added pro-oxidant TBH. The commercial availability of turmeric and ginger and their frequent combined use in diet suggest ideas for further broader utilization of the beneficial synergistic effect of their phenolic components.",
publisher = "MDPI",
journal = "Molecules",
title = "Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls",
volume = "28",
number = "6",
doi = "10.3390/molecules28062646"
}

Kostić, K., Brborić, J., Delogu, G., Simić, M., Samardžić, S., Maksimović, Z., Dettori, M. A., Fabbri, D., Kotur-Stevuljević, J.,& Saso, L.. (2023). Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls. in Molecules
MDPI., 28(6).
https://doi.org/10.3390/molecules28062646

Kostić K, Brborić J, Delogu G, Simić M, Samardžić S, Maksimović Z, Dettori MA, Fabbri D, Kotur-Stevuljević J, Saso L. Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls. in Molecules. 2023;28(6).
doi:10.3390/molecules28062646 .

Kostić, Kristina, Brborić, Jasmina, Delogu, Giovanna, Simić, Milena, Samardžić, Stevan, Maksimović, Zoran, Dettori, Maria Antonietta, Fabbri, Davide, Kotur-Stevuljević, Jelena, Saso, Luciano, "Antioxidant Activity of Natural Phenols and Derived Hydroxylated Biphenyls" in Molecules, 28, no. 6 (2023),
https://doi.org/10.3390/molecules28062646 . .

TY  - JOUR
AU  - Subošić, Branko
AU  - Kotur-Stevuljević, Jelena
AU  - Brborić, Jasmina
AU  - Janković, Tamara
AU  - Milenković, Marina
AU  - Ivković, Branka
AU  - Kostadinović, Jelena
AU  - Savić, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5146
AB  - The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen.
PB  - Pakistan Journal of Pharmaceutical Sciences
T2  - Pakistan Journal of Pharmaceutical Sciences
T1  - Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids
VL  - 36
IS  - 5
SP  - 1367
EP  - 1374
DO  - 10.36721/PJPS.2023.36.5.REG.1367-1374.1
ER  - 

@article{
author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Brborić, Jasmina and Janković, Tamara and Milenković, Marina and Ivković, Branka and Kostadinović, Jelena and Savić, Jelena",
year = "2023",
abstract = "The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen.",
publisher = "Pakistan Journal of Pharmaceutical Sciences",
journal = "Pakistan Journal of Pharmaceutical Sciences",
title = "Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids",
volume = "36",
number = "5",
pages = "1367-1374",
doi = "10.36721/PJPS.2023.36.5.REG.1367-1374.1"
}

Subošić, B., Kotur-Stevuljević, J., Brborić, J., Janković, T., Milenković, M., Ivković, B., Kostadinović, J.,& Savić, J.. (2023). Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids. in Pakistan Journal of Pharmaceutical Sciences
Pakistan Journal of Pharmaceutical Sciences., 36(5), 1367-1374.
https://doi.org/10.36721/PJPS.2023.36.5.REG.1367-1374.1

Subošić B, Kotur-Stevuljević J, Brborić J, Janković T, Milenković M, Ivković B, Kostadinović J, Savić J. Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids. in Pakistan Journal of Pharmaceutical Sciences. 2023;36(5):1367-1374.
doi:10.36721/PJPS.2023.36.5.REG.1367-1374.1 .

Subošić, Branko, Kotur-Stevuljević, Jelena, Brborić, Jasmina, Janković, Tamara, Milenković, Marina, Ivković, Branka, Kostadinović, Jelena, Savić, Jelena, "Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids" in Pakistan Journal of Pharmaceutical Sciences, 36, no. 5 (2023):1367-1374,
https://doi.org/10.36721/PJPS.2023.36.5.REG.1367-1374.1 . .

TY  - CONF
AU  - Zabelaj, Davor
AU  - Ivković, Branka
AU  - Čudina, Olivera
AU  - Brborić, Jasmina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4602
AB  - Flunixin is a non-steroidal anti-inflammatory drug, as well as an analgesic that has an
antipyretic effect. In veterinary medicine, it is used as salt flunixin meglumine. Flunixin
shows strong inhibition of the cyclooxygenase system involved in the development of
inflammation (1). The decrease in the production of certain inflammatory mediators explains
its analgesic, antipyretic and anti-inflammatory properties. A large number of methods have
been developed for the separation of flunixin meglumine and structurally related
compounds in pharmaceutical preparations, such as: thin layer chromatography, UV/VIS
spectroscopy, high pressure liquid chromatography and gas chromatography. The objective
of this work is to develop, optimize and validate a method for separating flunixin meglumine
and structurally related substances, in solution for injection. Optimal conditions were
achieved on a Agilent Zorbax Eclipse Plus C18 column (150×4.6 mm, 5 μm). The mobile
phase is a mixture of 0.1% (v/v) formic acid in water and methanol in a ratio of 40:60 (v/v).
The flow rate is 1.0 mL/min, the injection volume is 20 μL, the detection wavelength is 270
nm and the column temperature is 25°C. The following parameters were examined:
selectivity, linearity, precision, accuracy, detection limit, quantification limit, robustness and
stability of the solution (2). All these parameters were in line with the criteria for accepting
the results. Thereafter, the method was applied to determine the content of flunixin
meglumine and its impurities in the solution for injection, and the result was also in
accordance with the criteria for accepting the results.
AB  - Fluniksin je nesteroidni antiinflamatorni lek, kao i analgetik koji ima antipiretički
efekat. U veterinarskoj medicini se koristi u obliku soli fluniksin-meglumina. Fluniksin
pokazuje snažnu inhibiciju enzima ciklooksigenaze koji je uključen u nastanak inflamacije
(1). Smanjenjem produkcije određenih medijatora inflamacije objašnjavaju se njegova
analgetička, antipiretička i antiinflamatorna svojstva. Razvijen je veliki broj metoda za
odvajanje fluniksin meglumina i strukturno srodnih jedinjenja u farmaceutskim preparatima,
kao što su: tankoslojna hromatografija, UV/VIS spektroskopija, visokoefikasna tečna
hromatografija, gasna hromatografija. Cilj ovoga rada je razvoj, optimizacija i validacija
metode za razdvajanje i određivanje fluniksin-meglumina i strukturno sličnih jedinjenja, u
rastvoru za injekciju. Optimalni uslovi postignuti su na koloni Agilent Zorbax Eclipse Plus
C18 (150×4,6 mm, 5 μm). Mobilnu fazu činila je smeša 0,1% v/v rastvora mravlje kiseline u
vodi i metanola u odnosu 40:60 v/v. Protok mobilne faze je 1,0 mL/min, volumen injiciranja
20 μL, talasna dužina detekcije 270 nm i temperatura kolone 25°C. Ispitivani su sledeći
parametri: selektivnost, linearnost, preciznost, tačnost, limit detekcije, limit kvantifikacije,
robustnost i stabilnost rastvora (2). Dobijene vrednosti za ispitivane parametre su u skladu
sa kriterijumima prihvatljivosti. Validirana metoda je primenjena za određivanje sadržaja
fluniksin-meglumina i njegovih nečistoća u rastvoru za injekcije. Dobijeni rezultati
zadovoljavali su zahteve specifikacije.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Development and validation of high-performance liquid chromatography method for determination of flunixin-meglumine and its impurities in preparations for veterinary use
T1  - Razvoj i validacija metode tečne hromatografije visokih performansi za određivanje fluniksin‐meglumina i njegovih nečistoća u preparatima za veterinarsku upotrebu
VL  - 72
IS  - 4 suplement
SP  - S540
EP  - S541
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4602
ER  - 

@conference{
author = "Zabelaj, Davor and Ivković, Branka and Čudina, Olivera and Brborić, Jasmina",
year = "2022",
abstract = "Flunixin is a non-steroidal anti-inflammatory drug, as well as an analgesic that has an
antipyretic effect. In veterinary medicine, it is used as salt flunixin meglumine. Flunixin
shows strong inhibition of the cyclooxygenase system involved in the development of
inflammation (1). The decrease in the production of certain inflammatory mediators explains
its analgesic, antipyretic and anti-inflammatory properties. A large number of methods have
been developed for the separation of flunixin meglumine and structurally related
compounds in pharmaceutical preparations, such as: thin layer chromatography, UV/VIS
spectroscopy, high pressure liquid chromatography and gas chromatography. The objective
of this work is to develop, optimize and validate a method for separating flunixin meglumine
and structurally related substances, in solution for injection. Optimal conditions were
achieved on a Agilent Zorbax Eclipse Plus C18 column (150×4.6 mm, 5 μm). The mobile
phase is a mixture of 0.1% (v/v) formic acid in water and methanol in a ratio of 40:60 (v/v).
The flow rate is 1.0 mL/min, the injection volume is 20 μL, the detection wavelength is 270
nm and the column temperature is 25°C. The following parameters were examined:
selectivity, linearity, precision, accuracy, detection limit, quantification limit, robustness and
stability of the solution (2). All these parameters were in line with the criteria for accepting
the results. Thereafter, the method was applied to determine the content of flunixin
meglumine and its impurities in the solution for injection, and the result was also in
accordance with the criteria for accepting the results., Fluniksin je nesteroidni antiinflamatorni lek, kao i analgetik koji ima antipiretički
efekat. U veterinarskoj medicini se koristi u obliku soli fluniksin-meglumina. Fluniksin
pokazuje snažnu inhibiciju enzima ciklooksigenaze koji je uključen u nastanak inflamacije
(1). Smanjenjem produkcije određenih medijatora inflamacije objašnjavaju se njegova
analgetička, antipiretička i antiinflamatorna svojstva. Razvijen je veliki broj metoda za
odvajanje fluniksin meglumina i strukturno srodnih jedinjenja u farmaceutskim preparatima,
kao što su: tankoslojna hromatografija, UV/VIS spektroskopija, visokoefikasna tečna
hromatografija, gasna hromatografija. Cilj ovoga rada je razvoj, optimizacija i validacija
metode za razdvajanje i određivanje fluniksin-meglumina i strukturno sličnih jedinjenja, u
rastvoru za injekciju. Optimalni uslovi postignuti su na koloni Agilent Zorbax Eclipse Plus
C18 (150×4,6 mm, 5 μm). Mobilnu fazu činila je smeša 0,1% v/v rastvora mravlje kiseline u
vodi i metanola u odnosu 40:60 v/v. Protok mobilne faze je 1,0 mL/min, volumen injiciranja
20 μL, talasna dužina detekcije 270 nm i temperatura kolone 25°C. Ispitivani su sledeći
parametri: selektivnost, linearnost, preciznost, tačnost, limit detekcije, limit kvantifikacije,
robustnost i stabilnost rastvora (2). Dobijene vrednosti za ispitivane parametre su u skladu
sa kriterijumima prihvatljivosti. Validirana metoda je primenjena za određivanje sadržaja
fluniksin-meglumina i njegovih nečistoća u rastvoru za injekcije. Dobijeni rezultati
zadovoljavali su zahteve specifikacije.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Development and validation of high-performance liquid chromatography method for determination of flunixin-meglumine and its impurities in preparations for veterinary use, Razvoj i validacija metode tečne hromatografije visokih performansi za određivanje fluniksin‐meglumina i njegovih nečistoća u preparatima za veterinarsku upotrebu",
volume = "72",
number = "4 suplement",
pages = "S540-S541",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4602"
}

Zabelaj, D., Ivković, B., Čudina, O.,& Brborić, J.. (2022). Development and validation of high-performance liquid chromatography method for determination of flunixin-meglumine and its impurities in preparations for veterinary use. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S540-S541.
https://hdl.handle.net/21.15107/rcub_farfar_4602

Zabelaj D, Ivković B, Čudina O, Brborić J. Development and validation of high-performance liquid chromatography method for determination of flunixin-meglumine and its impurities in preparations for veterinary use. in Arhiv za farmaciju. 2022;72(4 suplement):S540-S541.
https://hdl.handle.net/21.15107/rcub_farfar_4602 .

Zabelaj, Davor, Ivković, Branka, Čudina, Olivera, Brborić, Jasmina, "Development and validation of high-performance liquid chromatography method for determination of flunixin-meglumine and its impurities in preparations for veterinary use" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S540-S541,
https://hdl.handle.net/21.15107/rcub_farfar_4602 .

TY  - CONF
AU  - Savić, Jelena
AU  - Brborić, Jasmina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4445
AB  - Nonsteroidal anti-inflammatory drugs (NSAIDs) have been used for nearly a century
for alleviation of symptoms of acute and chronic inflammation and represent one of the most
used groups of drugs in the general population. NSAIDs group is very numerous and it
includes different chemical structures. The main mechanism of action of these drugs is the
inhibition of enzyme cyclooxygenase (COX) which catalyzes prostaglandin production (of
which some are inflammatory mediators) from arachidonic acid and depending on whether
they inhibit both isoforms (COX-1 and COX-2) and just COX-2 they can be classified as
nonselective and selective. The need for finding the new NSAIDs with fewer side effects is
still persistent because nonselective NSAIDs often cause gastrointestinal side effects which
vary from mild to very serious like bleeding, while some selective are withdrawn because of
serious cardiovascular side effects with death outcome (1). Several epidemiologic studies
have shown a negative correlation between NSAID use and the occurrence of Alzheimer`s
disease, as well as some types of cancer, particularly colorectal and breast cancer. The
development of compounds that would be used in Alzheimer`s disease therapy is direct on
structures that exhibit more effects at the same time, one of which is anti-inflammatory
effect mediated via COX-2 inhibition. Although chemoprevention mechanisms are not
completely delineated, it is indisputable that both COX isoforms play a role in carcinogenesis,
and these findings opened a new field of research for the design and synthesis of new COX
inhibitors with chemoprotective, antiangiogenic, and cytotoxic activity.
AB  - Nesteroidni antiinflamatorni lekovi (NSAIL) se koriste za ublažavanje simptoma
akutne i hronične inflamacije već skoro čitav vek i predstavljaju jednu od najčešće
primenjivanih grupa lekova u najširoj populaciji. Grupa NSAIL je veoma brojna i obuhvata
različite hemijske strukture. Glavni mehanizam delovanja ovih lekova je inhibicija enzima
ciklooksigenaze (COX) koji katalizuje produkciju prostaglandina (od kojih su neki medijatori
inflamacije) iz arahidonske kiseline, a u zavisnosti od toga da li inhibiraju obe izoforme
(COX-1 i COX-2) ili samo COX-2, dele se na neselektivne i selektivne. Potreba za otkrivanjem
novih NSAIL sa manje neželjenih efekata je i danas aktuelna, jer neselektivni NSAIL često
izazivaju neželjene gastrointestinalne efekte koji variraju od blagih do veoma ozbiljnih kao
što je krvarenje, a neki selektivni lekovi su povučeni iz upotrebe zbog ozbiljnih neželjenih
kardiovaskularnih efekata sa smrtnim ishodom (1). Nekoliko epidemioloških studija je
pokazalo da postoji negativna korelacija između upotrebe NSAIL i pojave Alchajmerove
bolesti, kao i nekih tipova kancera, a naročito kolorektalnog i kancera dojke. Razvoj
jedinjenja koja bi se koristila u terapiji Alchajmerove bolesti je usmeren na strukture koje
istovremeno imaju više efekata, a jedan od njih je antiinflamatorni posredovan inhibicijom
COX-2. Iako mehanizmi hemoprevencije nisu potpuno rasvetljeni, nesporno je da postoji
uloga obe COX izoforme u karcinogenezi, a ova saznanja su otvorila novo polje istraživanja ka
dizajniranju i sintezi novih COX inhibitora sa hemoprotektivnom, antiangiogeneznom i
citotoksičnom aktivnošću.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - New directions in the development of cyclooxygenase inhibitors
T1  - Novi pravci u razvoju inhibitora ciklooksigenaze
VL  - 72
IS  - 4 suplement
SP  - S53
EP  - S54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4445
ER  - 

@conference{
author = "Savić, Jelena and Brborić, Jasmina",
year = "2022",
abstract = "Nonsteroidal anti-inflammatory drugs (NSAIDs) have been used for nearly a century
for alleviation of symptoms of acute and chronic inflammation and represent one of the most
used groups of drugs in the general population. NSAIDs group is very numerous and it
includes different chemical structures. The main mechanism of action of these drugs is the
inhibition of enzyme cyclooxygenase (COX) which catalyzes prostaglandin production (of
which some are inflammatory mediators) from arachidonic acid and depending on whether
they inhibit both isoforms (COX-1 and COX-2) and just COX-2 they can be classified as
nonselective and selective. The need for finding the new NSAIDs with fewer side effects is
still persistent because nonselective NSAIDs often cause gastrointestinal side effects which
vary from mild to very serious like bleeding, while some selective are withdrawn because of
serious cardiovascular side effects with death outcome (1). Several epidemiologic studies
have shown a negative correlation between NSAID use and the occurrence of Alzheimer`s
disease, as well as some types of cancer, particularly colorectal and breast cancer. The
development of compounds that would be used in Alzheimer`s disease therapy is direct on
structures that exhibit more effects at the same time, one of which is anti-inflammatory
effect mediated via COX-2 inhibition. Although chemoprevention mechanisms are not
completely delineated, it is indisputable that both COX isoforms play a role in carcinogenesis,
and these findings opened a new field of research for the design and synthesis of new COX
inhibitors with chemoprotective, antiangiogenic, and cytotoxic activity., Nesteroidni antiinflamatorni lekovi (NSAIL) se koriste za ublažavanje simptoma
akutne i hronične inflamacije već skoro čitav vek i predstavljaju jednu od najčešće
primenjivanih grupa lekova u najširoj populaciji. Grupa NSAIL je veoma brojna i obuhvata
različite hemijske strukture. Glavni mehanizam delovanja ovih lekova je inhibicija enzima
ciklooksigenaze (COX) koji katalizuje produkciju prostaglandina (od kojih su neki medijatori
inflamacije) iz arahidonske kiseline, a u zavisnosti od toga da li inhibiraju obe izoforme
(COX-1 i COX-2) ili samo COX-2, dele se na neselektivne i selektivne. Potreba za otkrivanjem
novih NSAIL sa manje neželjenih efekata je i danas aktuelna, jer neselektivni NSAIL često
izazivaju neželjene gastrointestinalne efekte koji variraju od blagih do veoma ozbiljnih kao
što je krvarenje, a neki selektivni lekovi su povučeni iz upotrebe zbog ozbiljnih neželjenih
kardiovaskularnih efekata sa smrtnim ishodom (1). Nekoliko epidemioloških studija je
pokazalo da postoji negativna korelacija između upotrebe NSAIL i pojave Alchajmerove
bolesti, kao i nekih tipova kancera, a naročito kolorektalnog i kancera dojke. Razvoj
jedinjenja koja bi se koristila u terapiji Alchajmerove bolesti je usmeren na strukture koje
istovremeno imaju više efekata, a jedan od njih je antiinflamatorni posredovan inhibicijom
COX-2. Iako mehanizmi hemoprevencije nisu potpuno rasvetljeni, nesporno je da postoji
uloga obe COX izoforme u karcinogenezi, a ova saznanja su otvorila novo polje istraživanja ka
dizajniranju i sintezi novih COX inhibitora sa hemoprotektivnom, antiangiogeneznom i
citotoksičnom aktivnošću.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "New directions in the development of cyclooxygenase inhibitors, Novi pravci u razvoju inhibitora ciklooksigenaze",
volume = "72",
number = "4 suplement",
pages = "S53-S54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4445"
}

Savić, J.,& Brborić, J.. (2022). New directions in the development of cyclooxygenase inhibitors. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S53-S54.
https://hdl.handle.net/21.15107/rcub_farfar_4445

Savić J, Brborić J. New directions in the development of cyclooxygenase inhibitors. in Arhiv za farmaciju. 2022;72(4 suplement):S53-S54.
https://hdl.handle.net/21.15107/rcub_farfar_4445 .

Savić, Jelena, Brborić, Jasmina, "New directions in the development of cyclooxygenase inhibitors" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S53-S54,
https://hdl.handle.net/21.15107/rcub_farfar_4445 .

TY  - CONF
AU  - Rupar, Jelena
AU  - Aleksić, Mara
AU  - Dobričić, Vladimir
AU  - Čudina, Olivera
AU  - Brborić, Jasmina
AU  - Gavrilov, Nemanja
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5482
AB  - Oxidation of newly synthesized acridine derivatives was studied using cyclic voltammetry at glassy
carbon electrode. Oxidation occurs as irreversible, diffusion-controlled process at pH 4.6 for
compounds 1-3 and as adsorption controlled process for compound 4. The interaction between newly
synthesized acridine compounds (compounds 1-4) and dsDNA was studied using a multilayer dsDNA
biosensor applying square wave voltammetry. Peak current corresponding to deoxyadenosine
decreased after 30 minutes of interaction suggesting interaction with dsDNA.
PB  - Society of Physical Chemists of Serbia
C3  - Physical Chemistry 2021 (Proceedings), 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021. Belgrade, Serbia
T1  - Electrochemical oxidation and interaction of newly synthesized acridine derivatives with DNA
VL  - I
SP  - 294
EP  - 297
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5482
ER  - 

@conference{
author = "Rupar, Jelena and Aleksić, Mara and Dobričić, Vladimir and Čudina, Olivera and Brborić, Jasmina and Gavrilov, Nemanja",
year = "2021",
abstract = "Oxidation of newly synthesized acridine derivatives was studied using cyclic voltammetry at glassy
carbon electrode. Oxidation occurs as irreversible, diffusion-controlled process at pH 4.6 for
compounds 1-3 and as adsorption controlled process for compound 4. The interaction between newly
synthesized acridine compounds (compounds 1-4) and dsDNA was studied using a multilayer dsDNA
biosensor applying square wave voltammetry. Peak current corresponding to deoxyadenosine
decreased after 30 minutes of interaction suggesting interaction with dsDNA.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical Chemistry 2021 (Proceedings), 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021. Belgrade, Serbia",
title = "Electrochemical oxidation and interaction of newly synthesized acridine derivatives with DNA",
volume = "I",
pages = "294-297",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5482"
}

Rupar, J., Aleksić, M., Dobričić, V., Čudina, O., Brborić, J.,& Gavrilov, N.. (2021). Electrochemical oxidation and interaction of newly synthesized acridine derivatives with DNA. in Physical Chemistry 2021 (Proceedings), 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021. Belgrade, Serbia
Society of Physical Chemists of Serbia., I, 294-297.
https://hdl.handle.net/21.15107/rcub_farfar_5482

Rupar J, Aleksić M, Dobričić V, Čudina O, Brborić J, Gavrilov N. Electrochemical oxidation and interaction of newly synthesized acridine derivatives with DNA. in Physical Chemistry 2021 (Proceedings), 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021. Belgrade, Serbia. 2021;I:294-297.
https://hdl.handle.net/21.15107/rcub_farfar_5482 .

Rupar, Jelena, Aleksić, Mara, Dobričić, Vladimir, Čudina, Olivera, Brborić, Jasmina, Gavrilov, Nemanja, "Electrochemical oxidation and interaction of newly synthesized acridine derivatives with DNA" in Physical Chemistry 2021 (Proceedings), 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 20-24, 2021. Belgrade, Serbia, I (2021):294-297,
https://hdl.handle.net/21.15107/rcub_farfar_5482 .

TY  - JOUR
AU  - Rupar, Jelena
AU  - Aleksić, Mara
AU  - Dobričić, Vladimir
AU  - Brborić, Jasmina
AU  - Čudina, Olivera
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3611
AB  - The electrochemical behavior of 9-chloroacridine (9Cl-A), a precursor molecule for synthesis of acridine derivatives with cytostatic activity, is a complex, pH-dependent, diffusion-controlled irreversible process. Oxidation of 9Cl-A initiates with the formation of a cation radical monomer, continues via the formation of a dimer subsequent oxidation to new cation radical. Reduction of 9Cl-A produces radical monomers which are stabilized by dimer formation. The investigation was performed using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The interaction between 9Cl-A and double-stranded DNA (dsDNA) was investigated using a multilayer dsDNA-electrochemical biosensor and 9Cl-A solutions from 1.0×10-7M (the lowest 9Cl-A concentration whose interaction with DNA was possible to detect) up to 1×10-4M. These allowed the binding constant, K=3.45×105M-1 and change in Gibbs free energy of the formed adsorbed complex to be calculated. Complex formation was a spontaneous process proceeding via 9Cl-A intercalation into dsDNA inducing structural changes. The intercalation of 9Cl-A into dsDNA was supported by molecular docking analysis. The combination of simple methodology and the use of biosensors to investigate DNA interactions is a powerful tool to offer insight into aspects of drug design during pharmaceutical development.
PB  - Elsevier B.V.
T2  - Bioelectrochemistry
T1  - An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA
VL  - 135
DO  - 10.1016/j.bioelechem.2020.107579
ER  - 

@article{
author = "Rupar, Jelena and Aleksić, Mara and Dobričić, Vladimir and Brborić, Jasmina and Čudina, Olivera",
year = "2020",
abstract = "The electrochemical behavior of 9-chloroacridine (9Cl-A), a precursor molecule for synthesis of acridine derivatives with cytostatic activity, is a complex, pH-dependent, diffusion-controlled irreversible process. Oxidation of 9Cl-A initiates with the formation of a cation radical monomer, continues via the formation of a dimer subsequent oxidation to new cation radical. Reduction of 9Cl-A produces radical monomers which are stabilized by dimer formation. The investigation was performed using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The interaction between 9Cl-A and double-stranded DNA (dsDNA) was investigated using a multilayer dsDNA-electrochemical biosensor and 9Cl-A solutions from 1.0×10-7M (the lowest 9Cl-A concentration whose interaction with DNA was possible to detect) up to 1×10-4M. These allowed the binding constant, K=3.45×105M-1 and change in Gibbs free energy of the formed adsorbed complex to be calculated. Complex formation was a spontaneous process proceeding via 9Cl-A intercalation into dsDNA inducing structural changes. The intercalation of 9Cl-A into dsDNA was supported by molecular docking analysis. The combination of simple methodology and the use of biosensors to investigate DNA interactions is a powerful tool to offer insight into aspects of drug design during pharmaceutical development.",
publisher = "Elsevier B.V.",
journal = "Bioelectrochemistry",
title = "An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA",
volume = "135",
doi = "10.1016/j.bioelechem.2020.107579"
}

Rupar, J., Aleksić, M., Dobričić, V., Brborić, J.,& Čudina, O.. (2020). An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA. in Bioelectrochemistry
Elsevier B.V.., 135.
https://doi.org/10.1016/j.bioelechem.2020.107579

Rupar J, Aleksić M, Dobričić V, Brborić J, Čudina O. An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA. in Bioelectrochemistry. 2020;135.
doi:10.1016/j.bioelechem.2020.107579 .

Rupar, Jelena, Aleksić, Mara, Dobričić, Vladimir, Brborić, Jasmina, Čudina, Olivera, "An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA" in Bioelectrochemistry, 135 (2020),
https://doi.org/10.1016/j.bioelechem.2020.107579 . .

TY  - JOUR
AU  - Rupar, Jelena
AU  - Dobričić, Vladimir
AU  - Grahovac, Jelena
AU  - Radulović, Siniša
AU  - Skok, Žiga
AU  - Ilaš, Janez
AU  - Aleksić, Mara
AU  - Brborić, Jasmina
AU  - Čudina, Olivera
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3606
AB  - A series of eleven 9-acridinyl amino acid derivatives were synthesized using a two-step procedure. Cytotoxicity was tested on the K562 and A549 cancer cell lines and normal diploid cell line MRC5 using the MTT assay. Compounds 6, 7, 8 and 9 were the most active, with IC50 values comparable to or lower than that of chemotherapeutic agent amsacrine. 8 and 9 were especially effective in the A549 cell line (IC50 ≈ 6 μM), which is of special interest since amsacrine is not sufficiently active in lung cancer patients. Cell cycle analysis revealed that 7 and 9 caused G2/M block, amsacrine caused arrest in the S phase, while 6 and 8 induced apoptotic cell death independently of the cell cycle regulation. In comparison to amsacrine, 6, 7, 8, and 9 showed similar inhibitory potential towards topoisomerase II, whereas only 7 showed DNA intercalation properties. In contrast to amsacrine, 6, 7, 8 and 9 showed a lack of toxicity towards unstimulated normal human leucocytes.
PB  - Royal Society of Chemistry
T2  - RSC Medicinal Chemistry
T1  - Synthesis and evaluation of anticancer activity of new 9-acridinyl amino acid derivatives
VL  - 11
IS  - 3
SP  - 378
EP  - 386
DO  - 10.1039/c9md00597h
DO  - 2-s2.0-85083014447
ER  - 

@article{
author = "Rupar, Jelena and Dobričić, Vladimir and Grahovac, Jelena and Radulović, Siniša and Skok, Žiga and Ilaš, Janez and Aleksić, Mara and Brborić, Jasmina and Čudina, Olivera",
year = "2020",
abstract = "A series of eleven 9-acridinyl amino acid derivatives were synthesized using a two-step procedure. Cytotoxicity was tested on the K562 and A549 cancer cell lines and normal diploid cell line MRC5 using the MTT assay. Compounds 6, 7, 8 and 9 were the most active, with IC50 values comparable to or lower than that of chemotherapeutic agent amsacrine. 8 and 9 were especially effective in the A549 cell line (IC50 ≈ 6 μM), which is of special interest since amsacrine is not sufficiently active in lung cancer patients. Cell cycle analysis revealed that 7 and 9 caused G2/M block, amsacrine caused arrest in the S phase, while 6 and 8 induced apoptotic cell death independently of the cell cycle regulation. In comparison to amsacrine, 6, 7, 8, and 9 showed similar inhibitory potential towards topoisomerase II, whereas only 7 showed DNA intercalation properties. In contrast to amsacrine, 6, 7, 8 and 9 showed a lack of toxicity towards unstimulated normal human leucocytes.",
publisher = "Royal Society of Chemistry",
journal = "RSC Medicinal Chemistry",
title = "Synthesis and evaluation of anticancer activity of new 9-acridinyl amino acid derivatives",
volume = "11",
number = "3",
pages = "378-386",
doi = "10.1039/c9md00597h, 2-s2.0-85083014447"
}

Rupar, J., Dobričić, V., Grahovac, J., Radulović, S., Skok, Ž., Ilaš, J., Aleksić, M., Brborić, J.,& Čudina, O.. (2020). Synthesis and evaluation of anticancer activity of new 9-acridinyl amino acid derivatives. in RSC Medicinal Chemistry
Royal Society of Chemistry., 11(3), 378-386.
https://doi.org/10.1039/c9md00597h

Rupar J, Dobričić V, Grahovac J, Radulović S, Skok Ž, Ilaš J, Aleksić M, Brborić J, Čudina O. Synthesis and evaluation of anticancer activity of new 9-acridinyl amino acid derivatives. in RSC Medicinal Chemistry. 2020;11(3):378-386.
doi:10.1039/c9md00597h .

Rupar, Jelena, Dobričić, Vladimir, Grahovac, Jelena, Radulović, Siniša, Skok, Žiga, Ilaš, Janez, Aleksić, Mara, Brborić, Jasmina, Čudina, Olivera, "Synthesis and evaluation of anticancer activity of new 9-acridinyl amino acid derivatives" in RSC Medicinal Chemistry, 11, no. 3 (2020):378-386,
https://doi.org/10.1039/c9md00597h . .

TY  - JOUR
AU  - Ivković, Branka
AU  - Brborić, Jasmina
AU  - Dobričić, Vladimir
AU  - Čudina, Olivera
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3355
AB  - A simple and convenient reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous separation, identification, and determination of sodium metabisulfite and sodium benzoate in pharmaceutical formulation has been developed and validated. Chromatographic separation was achieved on RP column Zorbax Extend C-18 (150 x 4.6 mm i.d., 3.5 mu m particles), and mixture of 0.1% phosphoric acid and acetonitrile in the ratio 62: 38 (v/v) was used as a mobile phase. The flow rate was set at 1.0 mL/min with detection wavelength of 275 nm. The method was successfully validated according to International Conference on Harmonization (ICH) guidelines acceptance criteria. The method is selective, as no interferences were observed at retention times corresponding to these analytes. Results of regression analyses (r) and statistical insignificance of calibration curve intercepts (p) proved linearity of the method in defined concentration ranges for sodium metabisulfite and sodium benzoate (0.05-0.15 mg/mL). Relative standard deviations calculated for both analytes in precision testing were below the limits defined for active pharmaceutical ingredients (analysis repeatability:  lt  2%; intermediate precision:  lt  3%). Recovery values were between 98.16% and 101.94%. According to results of robustness testing, chromatographic parameters are not significantly influenced by small variation of acetonitrile content in mobile phase, column temperature, and flow rate. Finally, the method was applied for quantitative determination of investigated preservatives in real sample analysis.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Chromatographica
T1  - Development and Validation of a New Isocratic RP-HPLC Method for Simultaneous Determination of Sodium Metabisulfite and Sodium Benzoate in Pharmaceutical Formulation
VL  - 31
IS  - 2
SP  - 133
EP  - 137
DO  - 10.1556/1326.2017.00404
ER  - 

@article{
author = "Ivković, Branka and Brborić, Jasmina and Dobričić, Vladimir and Čudina, Olivera",
year = "2019",
abstract = "A simple and convenient reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous separation, identification, and determination of sodium metabisulfite and sodium benzoate in pharmaceutical formulation has been developed and validated. Chromatographic separation was achieved on RP column Zorbax Extend C-18 (150 x 4.6 mm i.d., 3.5 mu m particles), and mixture of 0.1% phosphoric acid and acetonitrile in the ratio 62: 38 (v/v) was used as a mobile phase. The flow rate was set at 1.0 mL/min with detection wavelength of 275 nm. The method was successfully validated according to International Conference on Harmonization (ICH) guidelines acceptance criteria. The method is selective, as no interferences were observed at retention times corresponding to these analytes. Results of regression analyses (r) and statistical insignificance of calibration curve intercepts (p) proved linearity of the method in defined concentration ranges for sodium metabisulfite and sodium benzoate (0.05-0.15 mg/mL). Relative standard deviations calculated for both analytes in precision testing were below the limits defined for active pharmaceutical ingredients (analysis repeatability:  lt  2%; intermediate precision:  lt  3%). Recovery values were between 98.16% and 101.94%. According to results of robustness testing, chromatographic parameters are not significantly influenced by small variation of acetonitrile content in mobile phase, column temperature, and flow rate. Finally, the method was applied for quantitative determination of investigated preservatives in real sample analysis.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Chromatographica",
title = "Development and Validation of a New Isocratic RP-HPLC Method for Simultaneous Determination of Sodium Metabisulfite and Sodium Benzoate in Pharmaceutical Formulation",
volume = "31",
number = "2",
pages = "133-137",
doi = "10.1556/1326.2017.00404"
}

Ivković, B., Brborić, J., Dobričić, V.,& Čudina, O.. (2019). Development and Validation of a New Isocratic RP-HPLC Method for Simultaneous Determination of Sodium Metabisulfite and Sodium Benzoate in Pharmaceutical Formulation. in Acta Chromatographica
Akademiai Kiado Zrt, Budapest., 31(2), 133-137.
https://doi.org/10.1556/1326.2017.00404

Ivković B, Brborić J, Dobričić V, Čudina O. Development and Validation of a New Isocratic RP-HPLC Method for Simultaneous Determination of Sodium Metabisulfite and Sodium Benzoate in Pharmaceutical Formulation. in Acta Chromatographica. 2019;31(2):133-137.
doi:10.1556/1326.2017.00404 .

Ivković, Branka, Brborić, Jasmina, Dobričić, Vladimir, Čudina, Olivera, "Development and Validation of a New Isocratic RP-HPLC Method for Simultaneous Determination of Sodium Metabisulfite and Sodium Benzoate in Pharmaceutical Formulation" in Acta Chromatographica, 31, no. 2 (2019):133-137,
https://doi.org/10.1556/1326.2017.00404 . .

TY  - CONF
AU  - Dobričić, Vladimir
AU  - Savić, Jelena
AU  - Tubić, Biljana
AU  - Nikolić, Katarina
AU  - Brborić, Jasmina
AU  - Marković, Bojan
AU  - Čudina, Olivera
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5457
AB  - PAMPA (Parallel Artificial Membrane Permeability Assay) je brza i jednostavna in
vitro tehnika za procenu gastrointestinalne apsorpcije. Zasniva se na pasivnoj difuziji
ispitivanih supstanci kroz veštačku membranu koja simulira gastrointestinalni trakt.
QSPR (Quantitative Structure‐ Permeability Relationship Analysis) povezuje rezultate
PAMPA testa sa fizičko‐hemijskim osobinama ispitivanih jedinjenja, na osnovu čega je
moguć dizajn novih derivata sa poboljšanom apsorpcijom. Cilj rada je procena
gastrointestinalne apsorpcije trinaest β‐hidroksi‐β‐arilalkanskih kiselina sa
antiinflamatornom aktivnošću i četrnaest derivata 1,2‐etandiamina i 1,3‐
propandiamina sa antiproliferativnom aktivnošću primenom PAMPA testa, kao i dizajn
novih jedinjenja na osnovu QSPR analiza.
Gastrointestinalna apsorpcija je procenjena na hidrofobnim PVDF PAMPA
pločama, impregniranim 1% rastvorom lecitina jajeta u dodekanu (w/v). Molekulski
deskriptori ispitivanih jedinjenja su izračunati u programu Dragon i pomoću platforme
ChemDes. QSPR modeli su napravljeni u programima Simca 12+ P i STATISTICA.
Za sva ispitivana jedinjenja određeni su koeficijenti permeabilnosti (Papp)
primenom PAMPA testa, a za formiranje QSPR modela izračunati su i negativni
logaritmi ovih koeficijenata (‐logP app). Izdvojene su β‐hidroksi‐β‐arilalkanske kiseline
(1C, 1B i 2C), kao i derivati 1,2‐etandiamina i 1,3‐propandiamina (DM‐EDCP, EDCP i
DM‐PDCP) sa najvećom permeabilnošću kroz PAMPA membranu. Formirani su ANN‐,
MLR‐, PLS‐ i SVM‐QSPR modeli, pri čemu su najpouzdaniji modeli za predviđanje
permeabilnosti MLR(‐logP app) (za β‐hidroksi‐β‐arilalkanske kiseline), odnosno
PLS(‐logP app) (za derivate 1,2‐etandiamina i 1,3‐propandiamina). Na osnovu
deskriptora koji formiraju izdvojene modele predložene su strukturne promene koje bi
trebalo da poboljšaju permeabilnost kroz PAMPA veštačku membranu i
gastrointestinalnu apsorpciju.
Primenom PAMPA tehnike procenjena je gastrointestinalna apsorpcija trinaest
β‐hidroksi‐β‐arilalkanskih kiselina, kao i četrnaest derivata 1,2‐etandiamina i 1,3‐
propandiamina. Izdvojeni su derivati sa najvećom permeabilnošću i formirani su QSPR
modeli. Analizom najpouzdanijih modela, predložene su strukturne promene i
dizajnirani su novi derivati od kojih se može očekivati bolja gastrointestinalna
apsorpcija.
AB  - PAMPA (Parallel Artificial Membrane Permeability Assay) is a fast and simple in vitro technique used for the evaluation of gastrointestinal absorption. It is based on passive diffusion of tested substances through artificial membrane which simulates gastrointestinal tract. QSPR (Quantitative Structure‐Permeability Relationship Analysis) relates PAMPA results to physico‐chemical properties of tested compounds, which can be used for design of new derivatives with improved absorption. The aim of this work was evaluation of gastrointestinal absorption of thirteen β‐hydroxy‐β‐ arylalkanoic acids with antiinflammatory activity and fourteen derivatives of 1,2‐ ethanediamine and 1,3‐propanediamine with antiproliferative activity using PAMPA, as well as design of novel derivatives on the basis of QSPR analyses. Gastrointestinal absorption was evaluated using hydrophobic PAMPA plates impregnated with 1% egg lecithin solution in dodecane (w/v). Molecular descriptors of tested compounds were calculated using Dragon software and ChemDes platform. QSPR models were created in Simca 12+P and STATISTICA programs. Permeability coefficients (Papp) of all tested compounds were determined using PAMPA, whereas for QSPR modelling negative logarithms of these coefficients (‐logPapp) were calculated. β‐hydroxy‐β‐arylalkanoic acids (1C, 1B and 2C), as well as derivatives of 1,2‐ethanediamine and 1,3‐propanediamine (DM‐EDCP, EDCP and DM‐PDCP) with the highest PAMPA permeability were underlined. ANN‐, MLR‐, PLS‐ and SVM‐QSPR models were created, and the most reliable for permeability prediction were MLR(‐ logPapp) (β‐hydroxy‐β‐arylalkanoic acids) and PLS(‐logPapp) (derivatives of 1,2‐ ethanediamine and 1,3‐propanediamine). On the basis of descriptors that form selected models, structural modifications that should improve PAMPA permeability and gastrointestinal absorption were proposed. Gastrointestinal absorption of thirteen β‐hydroxy‐β‐arylalkanoic acids and fourteen derivatives of 1,2‐ethanediamine and 1,3‐propanediamine was evaluated using PAMPA technique. Derivatives with the highest permeability were underlined and QSPR models were created. After the analysis of the most reliable models, structural modifications were proposed and new derivatives with better expected gastrointestinal absorption were designed.
PB  - Savez farmaceutskih udruženja Srbije
C3  - Arhiv za farmaciju
T1  - Primena PAMPA tehnike i QSPR analize u proceni gastrointestinalne apsorpcije i dizajniranju novih biološki aktivnih jedinjenja
T1  - Application of PAMPA technique and QSPR analysis in the evaluation of gastrointestinal absorption and design of new biologically active compounds
VL  - 68
IS  - 2
SP  - 112
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5457
ER  - 

@conference{
author = "Dobričić, Vladimir and Savić, Jelena and Tubić, Biljana and Nikolić, Katarina and Brborić, Jasmina and Marković, Bojan and Čudina, Olivera",
year = "2018",
abstract = "PAMPA (Parallel Artificial Membrane Permeability Assay) je brza i jednostavna in
vitro tehnika za procenu gastrointestinalne apsorpcije. Zasniva se na pasivnoj difuziji
ispitivanih supstanci kroz veštačku membranu koja simulira gastrointestinalni trakt.
QSPR (Quantitative Structure‐ Permeability Relationship Analysis) povezuje rezultate
PAMPA testa sa fizičko‐hemijskim osobinama ispitivanih jedinjenja, na osnovu čega je
moguć dizajn novih derivata sa poboljšanom apsorpcijom. Cilj rada je procena
gastrointestinalne apsorpcije trinaest β‐hidroksi‐β‐arilalkanskih kiselina sa
antiinflamatornom aktivnošću i četrnaest derivata 1,2‐etandiamina i 1,3‐
propandiamina sa antiproliferativnom aktivnošću primenom PAMPA testa, kao i dizajn
novih jedinjenja na osnovu QSPR analiza.
Gastrointestinalna apsorpcija je procenjena na hidrofobnim PVDF PAMPA
pločama, impregniranim 1% rastvorom lecitina jajeta u dodekanu (w/v). Molekulski
deskriptori ispitivanih jedinjenja su izračunati u programu Dragon i pomoću platforme
ChemDes. QSPR modeli su napravljeni u programima Simca 12+ P i STATISTICA.
Za sva ispitivana jedinjenja određeni su koeficijenti permeabilnosti (Papp)
primenom PAMPA testa, a za formiranje QSPR modela izračunati su i negativni
logaritmi ovih koeficijenata (‐logP app). Izdvojene su β‐hidroksi‐β‐arilalkanske kiseline
(1C, 1B i 2C), kao i derivati 1,2‐etandiamina i 1,3‐propandiamina (DM‐EDCP, EDCP i
DM‐PDCP) sa najvećom permeabilnošću kroz PAMPA membranu. Formirani su ANN‐,
MLR‐, PLS‐ i SVM‐QSPR modeli, pri čemu su najpouzdaniji modeli za predviđanje
permeabilnosti MLR(‐logP app) (za β‐hidroksi‐β‐arilalkanske kiseline), odnosno
PLS(‐logP app) (za derivate 1,2‐etandiamina i 1,3‐propandiamina). Na osnovu
deskriptora koji formiraju izdvojene modele predložene su strukturne promene koje bi
trebalo da poboljšaju permeabilnost kroz PAMPA veštačku membranu i
gastrointestinalnu apsorpciju.
Primenom PAMPA tehnike procenjena je gastrointestinalna apsorpcija trinaest
β‐hidroksi‐β‐arilalkanskih kiselina, kao i četrnaest derivata 1,2‐etandiamina i 1,3‐
propandiamina. Izdvojeni su derivati sa najvećom permeabilnošću i formirani su QSPR
modeli. Analizom najpouzdanijih modela, predložene su strukturne promene i
dizajnirani su novi derivati od kojih se može očekivati bolja gastrointestinalna
apsorpcija., PAMPA (Parallel Artificial Membrane Permeability Assay) is a fast and simple in vitro technique used for the evaluation of gastrointestinal absorption. It is based on passive diffusion of tested substances through artificial membrane which simulates gastrointestinal tract. QSPR (Quantitative Structure‐Permeability Relationship Analysis) relates PAMPA results to physico‐chemical properties of tested compounds, which can be used for design of new derivatives with improved absorption. The aim of this work was evaluation of gastrointestinal absorption of thirteen β‐hydroxy‐β‐ arylalkanoic acids with antiinflammatory activity and fourteen derivatives of 1,2‐ ethanediamine and 1,3‐propanediamine with antiproliferative activity using PAMPA, as well as design of novel derivatives on the basis of QSPR analyses. Gastrointestinal absorption was evaluated using hydrophobic PAMPA plates impregnated with 1% egg lecithin solution in dodecane (w/v). Molecular descriptors of tested compounds were calculated using Dragon software and ChemDes platform. QSPR models were created in Simca 12+P and STATISTICA programs. Permeability coefficients (Papp) of all tested compounds were determined using PAMPA, whereas for QSPR modelling negative logarithms of these coefficients (‐logPapp) were calculated. β‐hydroxy‐β‐arylalkanoic acids (1C, 1B and 2C), as well as derivatives of 1,2‐ethanediamine and 1,3‐propanediamine (DM‐EDCP, EDCP and DM‐PDCP) with the highest PAMPA permeability were underlined. ANN‐, MLR‐, PLS‐ and SVM‐QSPR models were created, and the most reliable for permeability prediction were MLR(‐ logPapp) (β‐hydroxy‐β‐arylalkanoic acids) and PLS(‐logPapp) (derivatives of 1,2‐ ethanediamine and 1,3‐propanediamine). On the basis of descriptors that form selected models, structural modifications that should improve PAMPA permeability and gastrointestinal absorption were proposed. Gastrointestinal absorption of thirteen β‐hydroxy‐β‐arylalkanoic acids and fourteen derivatives of 1,2‐ethanediamine and 1,3‐propanediamine was evaluated using PAMPA technique. Derivatives with the highest permeability were underlined and QSPR models were created. After the analysis of the most reliable models, structural modifications were proposed and new derivatives with better expected gastrointestinal absorption were designed.",
publisher = "Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Primena PAMPA tehnike i QSPR analize u proceni gastrointestinalne apsorpcije i dizajniranju novih biološki aktivnih jedinjenja, Application of PAMPA technique and QSPR analysis in the evaluation of gastrointestinal absorption and design of new biologically active compounds",
volume = "68",
number = "2",
pages = "112-113",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5457"
}

Dobričić, V., Savić, J., Tubić, B., Nikolić, K., Brborić, J., Marković, B.,& Čudina, O.. (2018). Primena PAMPA tehnike i QSPR analize u proceni gastrointestinalne apsorpcije i dizajniranju novih biološki aktivnih jedinjenja. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije., 68(2), 112-113.
https://hdl.handle.net/21.15107/rcub_farfar_5457

Dobričić V, Savić J, Tubić B, Nikolić K, Brborić J, Marković B, Čudina O. Primena PAMPA tehnike i QSPR analize u proceni gastrointestinalne apsorpcije i dizajniranju novih biološki aktivnih jedinjenja. in Arhiv za farmaciju. 2018;68(2):112-113.
https://hdl.handle.net/21.15107/rcub_farfar_5457 .

Dobričić, Vladimir, Savić, Jelena, Tubić, Biljana, Nikolić, Katarina, Brborić, Jasmina, Marković, Bojan, Čudina, Olivera, "Primena PAMPA tehnike i QSPR analize u proceni gastrointestinalne apsorpcije i dizajniranju novih biološki aktivnih jedinjenja" in Arhiv za farmaciju, 68, no. 2 (2018):112-113,
https://hdl.handle.net/21.15107/rcub_farfar_5457 .

TY  - JOUR
AU  - Rupar, Jelena
AU  - Dobričić, Vladimir
AU  - Aleksić, Mara
AU  - Brborić, Jasmina
AU  - Čudina, Olivera
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3033
AB  - Acridine ring can be found in molecules used in many different spheres, including industry and medicine. Nowadays, even acridines with antibacterial activity are of research interest due to increasing bacterial resistance. Some acridine derivatives showed antimalarial or antiviral activity. Acridine derivatives were also investigated for antitumor activity due to the interaction with topoisomerase II and DNA base pairs. Considering these possible uses of acridine derivatives, this work was made as overview of all significant structure characteristics for specific action of these compounds.
PB  - Univerzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac
T2  - Kragujevac Journal of Science
T1  - A review of published data on acridine derivatives with different biological activities
IS  - 40
SP  - 83
EP  - 101
DO  - 10.5937/KgJSci1840083R
ER  - 

@article{
author = "Rupar, Jelena and Dobričić, Vladimir and Aleksić, Mara and Brborić, Jasmina and Čudina, Olivera",
year = "2018",
abstract = "Acridine ring can be found in molecules used in many different spheres, including industry and medicine. Nowadays, even acridines with antibacterial activity are of research interest due to increasing bacterial resistance. Some acridine derivatives showed antimalarial or antiviral activity. Acridine derivatives were also investigated for antitumor activity due to the interaction with topoisomerase II and DNA base pairs. Considering these possible uses of acridine derivatives, this work was made as overview of all significant structure characteristics for specific action of these compounds.",
publisher = "Univerzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac",
journal = "Kragujevac Journal of Science",
title = "A review of published data on acridine derivatives with different biological activities",
number = "40",
pages = "83-101",
doi = "10.5937/KgJSci1840083R"
}

Rupar, J., Dobričić, V., Aleksić, M., Brborić, J.,& Čudina, O.. (2018). A review of published data on acridine derivatives with different biological activities. in Kragujevac Journal of Science
Univerzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac.(40), 83-101.
https://doi.org/10.5937/KgJSci1840083R

Rupar J, Dobričić V, Aleksić M, Brborić J, Čudina O. A review of published data on acridine derivatives with different biological activities. in Kragujevac Journal of Science. 2018;(40):83-101.
doi:10.5937/KgJSci1840083R .

Rupar, Jelena, Dobričić, Vladimir, Aleksić, Mara, Brborić, Jasmina, Čudina, Olivera, "A review of published data on acridine derivatives with different biological activities" in Kragujevac Journal of Science, no. 40 (2018):83-101,
https://doi.org/10.5937/KgJSci1840083R . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dilber, Sanda
AU  - Crevar-Sakač, Milkica
AU  - Vladimirov, Sote
AU  - Brborić, Jasmina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3096
AB  - Lipophilicity parameters (logP) were determined for thirteen synthesized β-hydroxy-β-arilalkanoic acids using reversed phase high performance liquid chromatography. Anti-inflammatory activity and potential selectivity towards cyclooxygenase-2 inhibition of synthetized compounds was assessed. Stationary phase was octadecyl modified (C-18) silicagel, and four used mobile phases contained different amount of methanol. Both synthetized and standard compounds with known logP values (aspirin, ibuprofen, ketoprofen, naproxen and phenanthrene) were tested in a chromatographic system. Using retention times for each standard and synthesized compound logk values (logarithm of capacity factor) were calculated. Intercept on a graph showing dependency of logk from methanol amount in the mobile phase for each compound represents logKw(capacity factor when organic solvent amount is zero). Graph showing linear dependency of logP of standard compounds from experimentally obtained logKw values was plotted. LogP values for synthetized compounds were obtained by interpolation from the plotted graph. Obtained values are in a range from 2.901 to 3.847. The best correlation between experimentally obtained and predicted logP values was using KOWWIN software (R2=0.8864), which makes this software appropriate for predicting logP values of this type of compounds.
AB  - Parametri lipofilnosti (logP) su određeni za trinaest sintetisanih β-hidroksi-β-arilalkanskih kiselina primenom reverzno fazne tečne hromatografije. Ispitivanje je urađeno na derivatima kojima je tokom prethodnih istraživanja okarakterisana antiinflamatorna aktivnost i pretpostavljena je potencijalna selektivnost prema inhibiciji ciklooksigenaze-2. Oktadecil-modifikovani (C-18) silikagel je predstavljao stacionarnu fazu, a korišćene su četiri mobilne faze u kojima je variran udeo metanola. Hromatografski su testirana jedinjenja sa poznatim logP vrednostima (aspirin, ibuprofen, ketoprofen, naproksen i fenantren) i sintetisana jedinjenja. Na osnovu retencionog vremena za svako standardno i sintetisano jedinjenje izračunate su vrednosti logk (logaritam faktora kapaciteta). Odsečak na y osi grafika zavisnosti logk od udela metanola u mobilnoj fazi za svako jedinjenje predstavlja vrednost logKw (vrednost retencionog faktora za hromatografski sistem u kome je sadržaj organske komponente nula) za dato jedinjenje. Konstruisan je grafik zavisnosti logP za standardna jedinjenja od njihovih eksperimentalno dobijenih logKw vrednosti i uspostavljena je linearna zavisnost. Interpolacijom logKw sa grafika su očitane vrednosti logP za sintetisana jedinjenja. Dobijene vrednosti su u opsegu od 2,901 do 3,847. Za predviđanje logP vrednosti korišćeni su računarski programi: AlogPS, Molinspiration, MarvinSketch i KOWWIN. Najbolja korelacija između eksperimentalno određenih i predviđenih rezultata je u programu KOWWIN (R2=0,8864), što čini ovaj program pogodnim za predviđanje logP vrednosti ovog tipa jedinjenja.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Lipophilicity determination of β-hydroxy-β-arilalkanoic acids by reversed phase liquid chromatography under high pressure
T1  - Određivanje lipofilnosti β-hidroksi-β-arilalkanskih kiselina primenom reverzno-fazne tečne hromatografije pod visokim pritiskom
VL  - 68
IS  - 1
SP  - 34
EP  - 45
DO  - 10.5937/arhFarm1801034S
ER  - 

@article{
author = "Savić, Jelena and Dilber, Sanda and Crevar-Sakač, Milkica and Vladimirov, Sote and Brborić, Jasmina",
year = "2018",
abstract = "Lipophilicity parameters (logP) were determined for thirteen synthesized β-hydroxy-β-arilalkanoic acids using reversed phase high performance liquid chromatography. Anti-inflammatory activity and potential selectivity towards cyclooxygenase-2 inhibition of synthetized compounds was assessed. Stationary phase was octadecyl modified (C-18) silicagel, and four used mobile phases contained different amount of methanol. Both synthetized and standard compounds with known logP values (aspirin, ibuprofen, ketoprofen, naproxen and phenanthrene) were tested in a chromatographic system. Using retention times for each standard and synthesized compound logk values (logarithm of capacity factor) were calculated. Intercept on a graph showing dependency of logk from methanol amount in the mobile phase for each compound represents logKw(capacity factor when organic solvent amount is zero). Graph showing linear dependency of logP of standard compounds from experimentally obtained logKw values was plotted. LogP values for synthetized compounds were obtained by interpolation from the plotted graph. Obtained values are in a range from 2.901 to 3.847. The best correlation between experimentally obtained and predicted logP values was using KOWWIN software (R2=0.8864), which makes this software appropriate for predicting logP values of this type of compounds., Parametri lipofilnosti (logP) su određeni za trinaest sintetisanih β-hidroksi-β-arilalkanskih kiselina primenom reverzno fazne tečne hromatografije. Ispitivanje je urađeno na derivatima kojima je tokom prethodnih istraživanja okarakterisana antiinflamatorna aktivnost i pretpostavljena je potencijalna selektivnost prema inhibiciji ciklooksigenaze-2. Oktadecil-modifikovani (C-18) silikagel je predstavljao stacionarnu fazu, a korišćene su četiri mobilne faze u kojima je variran udeo metanola. Hromatografski su testirana jedinjenja sa poznatim logP vrednostima (aspirin, ibuprofen, ketoprofen, naproksen i fenantren) i sintetisana jedinjenja. Na osnovu retencionog vremena za svako standardno i sintetisano jedinjenje izračunate su vrednosti logk (logaritam faktora kapaciteta). Odsečak na y osi grafika zavisnosti logk od udela metanola u mobilnoj fazi za svako jedinjenje predstavlja vrednost logKw (vrednost retencionog faktora za hromatografski sistem u kome je sadržaj organske komponente nula) za dato jedinjenje. Konstruisan je grafik zavisnosti logP za standardna jedinjenja od njihovih eksperimentalno dobijenih logKw vrednosti i uspostavljena je linearna zavisnost. Interpolacijom logKw sa grafika su očitane vrednosti logP za sintetisana jedinjenja. Dobijene vrednosti su u opsegu od 2,901 do 3,847. Za predviđanje logP vrednosti korišćeni su računarski programi: AlogPS, Molinspiration, MarvinSketch i KOWWIN. Najbolja korelacija između eksperimentalno određenih i predviđenih rezultata je u programu KOWWIN (R2=0,8864), što čini ovaj program pogodnim za predviđanje logP vrednosti ovog tipa jedinjenja.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Lipophilicity determination of β-hydroxy-β-arilalkanoic acids by reversed phase liquid chromatography under high pressure, Određivanje lipofilnosti β-hidroksi-β-arilalkanskih kiselina primenom reverzno-fazne tečne hromatografije pod visokim pritiskom",
volume = "68",
number = "1",
pages = "34-45",
doi = "10.5937/arhFarm1801034S"
}

Savić, J., Dilber, S., Crevar-Sakač, M., Vladimirov, S.,& Brborić, J.. (2018). Lipophilicity determination of β-hydroxy-β-arilalkanoic acids by reversed phase liquid chromatography under high pressure. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 68(1), 34-45.
https://doi.org/10.5937/arhFarm1801034S

Savić J, Dilber S, Crevar-Sakač M, Vladimirov S, Brborić J. Lipophilicity determination of β-hydroxy-β-arilalkanoic acids by reversed phase liquid chromatography under high pressure. in Arhiv za farmaciju. 2018;68(1):34-45.
doi:10.5937/arhFarm1801034S .

Savić, Jelena, Dilber, Sanda, Crevar-Sakač, Milkica, Vladimirov, Sote, Brborić, Jasmina, "Lipophilicity determination of β-hydroxy-β-arilalkanoic acids by reversed phase liquid chromatography under high pressure" in Arhiv za farmaciju, 68, no. 1 (2018):34-45,
https://doi.org/10.5937/arhFarm1801034S . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dilber, Sanda
AU  - Vujić, Zorica
AU  - Vladimirov, Sote
AU  - Brborić, Jasmina
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3069
AB  - The pK(a) values of twelve beta-hydroxy-beta-arylalkanoic acids and ibuprofen were determined using a modified RP-HPLC method. The stationary phase was octadecyl modified (C-18) silica gel, and the mobile phases were mixtures of methanol and one of ten different buffers (60: 40 volume ratio). The mean retention time of each compound was plotted against the pH of each of the ten used mobile phases. The inflection point of the obtained sigmoidal curve represents the (s)(w)pK(a) of a compound. Using (s)(w)pKa in previously established equations for the specific methanol/buffer mixture, the (w)(w)pK(a) values (in pure water) were calculated. The obtained wwpKa values for the synthesized compounds were in a range from 3.40 to 3.74, and the (w)(w)pK(a) for ibuprofen was 4.27. The Predicted pK(a) values for this type of compounds in the MarvinSketch 5.11.5. Program were in poor correlation with the experimental results, while in ACD//I-Labs pK(a) values were calculated as a wide range.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - A modified RP-HPLC method for the determination of the pK(a) values of synthesized beta-hydroxy-beta-arylalkanoic acids
VL  - 83
IS  - 7-8
SP  - 875
EP  - 883
DO  - 10.2298/JSC170804045S
ER  - 

@article{
author = "Savić, Jelena and Dilber, Sanda and Vujić, Zorica and Vladimirov, Sote and Brborić, Jasmina",
year = "2018",
abstract = "The pK(a) values of twelve beta-hydroxy-beta-arylalkanoic acids and ibuprofen were determined using a modified RP-HPLC method. The stationary phase was octadecyl modified (C-18) silica gel, and the mobile phases were mixtures of methanol and one of ten different buffers (60: 40 volume ratio). The mean retention time of each compound was plotted against the pH of each of the ten used mobile phases. The inflection point of the obtained sigmoidal curve represents the (s)(w)pK(a) of a compound. Using (s)(w)pKa in previously established equations for the specific methanol/buffer mixture, the (w)(w)pK(a) values (in pure water) were calculated. The obtained wwpKa values for the synthesized compounds were in a range from 3.40 to 3.74, and the (w)(w)pK(a) for ibuprofen was 4.27. The Predicted pK(a) values for this type of compounds in the MarvinSketch 5.11.5. Program were in poor correlation with the experimental results, while in ACD//I-Labs pK(a) values were calculated as a wide range.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "A modified RP-HPLC method for the determination of the pK(a) values of synthesized beta-hydroxy-beta-arylalkanoic acids",
volume = "83",
number = "7-8",
pages = "875-883",
doi = "10.2298/JSC170804045S"
}

Savić, J., Dilber, S., Vujić, Z., Vladimirov, S.,& Brborić, J.. (2018). A modified RP-HPLC method for the determination of the pK(a) values of synthesized beta-hydroxy-beta-arylalkanoic acids. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 83(7-8), 875-883.
https://doi.org/10.2298/JSC170804045S

Savić J, Dilber S, Vujić Z, Vladimirov S, Brborić J. A modified RP-HPLC method for the determination of the pK(a) values of synthesized beta-hydroxy-beta-arylalkanoic acids. in Journal of the Serbian Chemical Society. 2018;83(7-8):875-883.
doi:10.2298/JSC170804045S .

Savić, Jelena, Dilber, Sanda, Vujić, Zorica, Vladimirov, Sote, Brborić, Jasmina, "A modified RP-HPLC method for the determination of the pK(a) values of synthesized beta-hydroxy-beta-arylalkanoic acids" in Journal of the Serbian Chemical Society, 83, no. 7-8 (2018):875-883,
https://doi.org/10.2298/JSC170804045S . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dobričić, Vladimir
AU  - Nikolić, Katarina
AU  - Vladimirov, Sote
AU  - Dilber, Sanda
AU  - Brborić, Jasmina
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2923
AB  - Prediction of gastrointestinal absorption of thirteen newly synthesized beta-hydroxy-beta-arylalkanoic acids (HAA) and ibuprofen was performed using PAMPA test The highest values of PAMPA parameters (%Tand P-app) were calculated for 1C, 1B and 2C and these parameters were significantly lower in comparison to ibuprofen. QSPR analysis was performed in order to identify molecular descriptors with the highest influence on %Tand-logP(app) and to create models which could be used for the design of novel HAA with improved gastrointestinal absorption. Obtained results indicate that introduction of branched side chain, as well as introduction of substituents on one phenyl ring (which disturb symmetry of the molecule) could have positive impact on gastrointestinal absorption. On the basis of these results, six novel HAA were designed and PAMPA parameters %Tand-logP(app) were predicted by use of selected QSPR models. Designed derivatives should have better gastrointestinal absorption than HAA tested in this study.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - In vitro prediction of gastrointestinal absorption of novel beta-hydroxy-beta-arylalkanoic acids using PAMPA technique
VL  - 100
SP  - 36
EP  - 41
DO  - 10.1016/j.ejps.2017.01.005
ER  - 

@article{
author = "Savić, Jelena and Dobričić, Vladimir and Nikolić, Katarina and Vladimirov, Sote and Dilber, Sanda and Brborić, Jasmina",
year = "2017",
abstract = "Prediction of gastrointestinal absorption of thirteen newly synthesized beta-hydroxy-beta-arylalkanoic acids (HAA) and ibuprofen was performed using PAMPA test The highest values of PAMPA parameters (%Tand P-app) were calculated for 1C, 1B and 2C and these parameters were significantly lower in comparison to ibuprofen. QSPR analysis was performed in order to identify molecular descriptors with the highest influence on %Tand-logP(app) and to create models which could be used for the design of novel HAA with improved gastrointestinal absorption. Obtained results indicate that introduction of branched side chain, as well as introduction of substituents on one phenyl ring (which disturb symmetry of the molecule) could have positive impact on gastrointestinal absorption. On the basis of these results, six novel HAA were designed and PAMPA parameters %Tand-logP(app) were predicted by use of selected QSPR models. Designed derivatives should have better gastrointestinal absorption than HAA tested in this study.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "In vitro prediction of gastrointestinal absorption of novel beta-hydroxy-beta-arylalkanoic acids using PAMPA technique",
volume = "100",
pages = "36-41",
doi = "10.1016/j.ejps.2017.01.005"
}

Savić, J., Dobričić, V., Nikolić, K., Vladimirov, S., Dilber, S.,& Brborić, J.. (2017). In vitro prediction of gastrointestinal absorption of novel beta-hydroxy-beta-arylalkanoic acids using PAMPA technique. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 100, 36-41.
https://doi.org/10.1016/j.ejps.2017.01.005

Savić J, Dobričić V, Nikolić K, Vladimirov S, Dilber S, Brborić J. In vitro prediction of gastrointestinal absorption of novel beta-hydroxy-beta-arylalkanoic acids using PAMPA technique. in European Journal of Pharmaceutical Sciences. 2017;100:36-41.
doi:10.1016/j.ejps.2017.01.005 .

Savić, Jelena, Dobričić, Vladimir, Nikolić, Katarina, Vladimirov, Sote, Dilber, Sanda, Brborić, Jasmina, "In vitro prediction of gastrointestinal absorption of novel beta-hydroxy-beta-arylalkanoic acids using PAMPA technique" in European Journal of Pharmaceutical Sciences, 100 (2017):36-41,
https://doi.org/10.1016/j.ejps.2017.01.005 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Savić, Jelena
AU  - Nikolić, Katarina
AU  - Vladimirov, Sote
AU  - Vujić, Zorica
AU  - Brborić, Jasmina
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2913
AB  - Gastrointestinal absorption of thirteen novel beta-hydroxy-beta-arylalkanoic acids (HAA) with anti-inflammatory activity was predicted by use of biopartitioning micellar chromatography and compared to ibuprofen. All tested HAA have lower retention factors (k) and lower expected gastrointestinal absorption than ibuprofen, whereas derivatives with the highest values of k are 1C, 2APTF and 2C. Quantitative structure-retention relationship (QSRR) analysis was performed in order to identify molecular descriptors with the highest influence on k and ANN(k) model was selected as optimal. Descriptors which form this model (nBM, P VSA_LogP_8 and Eta_L) indicate that replacement of phenyl ring with a saturated or partially unsaturated one, as well as presence of halogens and nitro group should positively affect k values. On the basis of these conclusions, six novel HAA were designed and selected QSRR model was used for the prediction of their k values.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Application of biopartitioning micellar chromatography and QSRR modeling for prediction of gastrointestinal absorption and design of novel beta-hydroxy-beta-arylalkanoic acids
VL  - 100
SP  - 280
EP  - 284
DO  - 10.1016/j.ejps.2017.01.023
ER  - 

@article{
author = "Dobričić, Vladimir and Savić, Jelena and Nikolić, Katarina and Vladimirov, Sote and Vujić, Zorica and Brborić, Jasmina",
year = "2017",
abstract = "Gastrointestinal absorption of thirteen novel beta-hydroxy-beta-arylalkanoic acids (HAA) with anti-inflammatory activity was predicted by use of biopartitioning micellar chromatography and compared to ibuprofen. All tested HAA have lower retention factors (k) and lower expected gastrointestinal absorption than ibuprofen, whereas derivatives with the highest values of k are 1C, 2APTF and 2C. Quantitative structure-retention relationship (QSRR) analysis was performed in order to identify molecular descriptors with the highest influence on k and ANN(k) model was selected as optimal. Descriptors which form this model (nBM, P VSA_LogP_8 and Eta_L) indicate that replacement of phenyl ring with a saturated or partially unsaturated one, as well as presence of halogens and nitro group should positively affect k values. On the basis of these conclusions, six novel HAA were designed and selected QSRR model was used for the prediction of their k values.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Application of biopartitioning micellar chromatography and QSRR modeling for prediction of gastrointestinal absorption and design of novel beta-hydroxy-beta-arylalkanoic acids",
volume = "100",
pages = "280-284",
doi = "10.1016/j.ejps.2017.01.023"
}

Dobričić, V., Savić, J., Nikolić, K., Vladimirov, S., Vujić, Z.,& Brborić, J.. (2017). Application of biopartitioning micellar chromatography and QSRR modeling for prediction of gastrointestinal absorption and design of novel beta-hydroxy-beta-arylalkanoic acids. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 100, 280-284.
https://doi.org/10.1016/j.ejps.2017.01.023

Dobričić V, Savić J, Nikolić K, Vladimirov S, Vujić Z, Brborić J. Application of biopartitioning micellar chromatography and QSRR modeling for prediction of gastrointestinal absorption and design of novel beta-hydroxy-beta-arylalkanoic acids. in European Journal of Pharmaceutical Sciences. 2017;100:280-284.
doi:10.1016/j.ejps.2017.01.023 .

Dobričić, Vladimir, Savić, Jelena, Nikolić, Katarina, Vladimirov, Sote, Vujić, Zorica, Brborić, Jasmina, "Application of biopartitioning micellar chromatography and QSRR modeling for prediction of gastrointestinal absorption and design of novel beta-hydroxy-beta-arylalkanoic acids" in European Journal of Pharmaceutical Sciences, 100 (2017):280-284,
https://doi.org/10.1016/j.ejps.2017.01.023 . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dilber, Sanda
AU  - Milenković, Marina
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Bojan
AU  - Vladimirov, Sote
AU  - Brborić, Jasmina
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2891
AB  - Background: Nonsteriodal anti-inflammatory drugs (NSAIDs) are numerous and widely used for more than 60 years, but there is still a strong need for developing novel selective NSAIDs. The need is justified by the fact that nonselective NSAIDs can produce serious gastric side effects and that some of the selective NSAID are withdrawn due to their cardiotoxic side effects. Methods: Eight beta-hydroxy-beta-arylpropanoic acids, which belong to the arylpropanoic acid class of compounds, structurally similar to some nonsteroidal anti-inflammatory drugs (NSAIDs), were docked into 3D catalytic site of both cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Seven out of those eight acids were synthesized using already published modification of Reformatsky reaction additionally optimized by increasing temperature. Synthesized compounds were tested in vivo in order to elucidate anti-inflammatory activity, gastric tolerability and impact on liver function of rats. Results: Results of docking studies have indicated that all compounds have potential to selectively inhibit COX-2 isoform, but that the compounds containing polar substituents on phenyl ring are better inhibitors. Results of carrageenan-induced rat paw oedema test have shown that all compounds exhibit dose dependence and good gastric tolerability and none of the tested compounds have shown negative effect on liver function compared to ibuprofen. Conclusion: The compound containing polar nitro group in para position has shown the best docking results, anti-inflammatory activity, low hepatotoxicity and good gastric tolerability.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Medicinal Chemistry
T1  - Docking Studies, Synthesis and Biological Evaluation of beta-aryl-beta-hydroxy Propanoic Acids for Anti-inflammatory Activity
VL  - 13
IS  - 2
SP  - 186
EP  - 195
DO  - 10.2174/1573406412666160907150247
ER  - 

@article{
author = "Savić, Jelena and Dilber, Sanda and Milenković, Marina and Kotur-Stevuljević, Jelena and Marković, Bojan and Vladimirov, Sote and Brborić, Jasmina",
year = "2017",
abstract = "Background: Nonsteriodal anti-inflammatory drugs (NSAIDs) are numerous and widely used for more than 60 years, but there is still a strong need for developing novel selective NSAIDs. The need is justified by the fact that nonselective NSAIDs can produce serious gastric side effects and that some of the selective NSAID are withdrawn due to their cardiotoxic side effects. Methods: Eight beta-hydroxy-beta-arylpropanoic acids, which belong to the arylpropanoic acid class of compounds, structurally similar to some nonsteroidal anti-inflammatory drugs (NSAIDs), were docked into 3D catalytic site of both cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Seven out of those eight acids were synthesized using already published modification of Reformatsky reaction additionally optimized by increasing temperature. Synthesized compounds were tested in vivo in order to elucidate anti-inflammatory activity, gastric tolerability and impact on liver function of rats. Results: Results of docking studies have indicated that all compounds have potential to selectively inhibit COX-2 isoform, but that the compounds containing polar substituents on phenyl ring are better inhibitors. Results of carrageenan-induced rat paw oedema test have shown that all compounds exhibit dose dependence and good gastric tolerability and none of the tested compounds have shown negative effect on liver function compared to ibuprofen. Conclusion: The compound containing polar nitro group in para position has shown the best docking results, anti-inflammatory activity, low hepatotoxicity and good gastric tolerability.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Medicinal Chemistry",
title = "Docking Studies, Synthesis and Biological Evaluation of beta-aryl-beta-hydroxy Propanoic Acids for Anti-inflammatory Activity",
volume = "13",
number = "2",
pages = "186-195",
doi = "10.2174/1573406412666160907150247"
}

Savić, J., Dilber, S., Milenković, M., Kotur-Stevuljević, J., Marković, B., Vladimirov, S.,& Brborić, J.. (2017). Docking Studies, Synthesis and Biological Evaluation of beta-aryl-beta-hydroxy Propanoic Acids for Anti-inflammatory Activity. in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 13(2), 186-195.
https://doi.org/10.2174/1573406412666160907150247

Savić J, Dilber S, Milenković M, Kotur-Stevuljević J, Marković B, Vladimirov S, Brborić J. Docking Studies, Synthesis and Biological Evaluation of beta-aryl-beta-hydroxy Propanoic Acids for Anti-inflammatory Activity. in Medicinal Chemistry. 2017;13(2):186-195.
doi:10.2174/1573406412666160907150247 .

Savić, Jelena, Dilber, Sanda, Milenković, Marina, Kotur-Stevuljević, Jelena, Marković, Bojan, Vladimirov, Sote, Brborić, Jasmina, "Docking Studies, Synthesis and Biological Evaluation of beta-aryl-beta-hydroxy Propanoic Acids for Anti-inflammatory Activity" in Medicinal Chemistry, 13, no. 2 (2017):186-195,
https://doi.org/10.2174/1573406412666160907150247 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Brborić, Jasmina
AU  - Vujić, Zorica
AU  - Uskoković-Marković, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2718
AB  - Many of modern diagnostic methods cannot be achieved without radioisotopes. In this review, an overview of the most common radioisotopes (radionuclides) used as markers for in vitro and in vivo studies is provided. To determine the extremely low concentrations of some biological molecules (hormones, drugs, toxins, nucleic acid, etc.) in biological fluids for in vitro studies, β and/or γ emitters with different half-lives: H-3, C-14, P-32, P-33, I-125, Cr-51 were frequently used. The majority of all radionuclides (90 %) were utilized for in vivo diagnostics. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are imaging modalities widely used in diagnostic nuclear medicine. The most important diagnostic radionuclides are technetium-99m (for single photon emission computed tomography) and fluorine-18 (for positron emission tomography).
PB  - Chemical Publishing Co.
T2  - Asian Journal of Chemistry
T1  - Radioisotopes used as radiotracers for in vitro and in vivo diagnostics
VL  - 28
IS  - 2
SP  - 235
EP  - 241
DO  - 10.14233/ajchem.2016.19401
ER  - 

@article{
author = "Kuntić, Vesna and Brborić, Jasmina and Vujić, Zorica and Uskoković-Marković, Snežana",
year = "2016",
abstract = "Many of modern diagnostic methods cannot be achieved without radioisotopes. In this review, an overview of the most common radioisotopes (radionuclides) used as markers for in vitro and in vivo studies is provided. To determine the extremely low concentrations of some biological molecules (hormones, drugs, toxins, nucleic acid, etc.) in biological fluids for in vitro studies, β and/or γ emitters with different half-lives: H-3, C-14, P-32, P-33, I-125, Cr-51 were frequently used. The majority of all radionuclides (90 %) were utilized for in vivo diagnostics. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are imaging modalities widely used in diagnostic nuclear medicine. The most important diagnostic radionuclides are technetium-99m (for single photon emission computed tomography) and fluorine-18 (for positron emission tomography).",
publisher = "Chemical Publishing Co.",
journal = "Asian Journal of Chemistry",
title = "Radioisotopes used as radiotracers for in vitro and in vivo diagnostics",
volume = "28",
number = "2",
pages = "235-241",
doi = "10.14233/ajchem.2016.19401"
}

Kuntić, V., Brborić, J., Vujić, Z.,& Uskoković-Marković, S.. (2016). Radioisotopes used as radiotracers for in vitro and in vivo diagnostics. in Asian Journal of Chemistry
Chemical Publishing Co.., 28(2), 235-241.
https://doi.org/10.14233/ajchem.2016.19401

Kuntić V, Brborić J, Vujić Z, Uskoković-Marković S. Radioisotopes used as radiotracers for in vitro and in vivo diagnostics. in Asian Journal of Chemistry. 2016;28(2):235-241.
doi:10.14233/ajchem.2016.19401 .

Kuntić, Vesna, Brborić, Jasmina, Vujić, Zorica, Uskoković-Marković, Snežana, "Radioisotopes used as radiotracers for in vitro and in vivo diagnostics" in Asian Journal of Chemistry, 28, no. 2 (2016):235-241,
https://doi.org/10.14233/ajchem.2016.19401 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Brborić, Jasmina
AU  - Holclajtner-Antunović, Ivanka
AU  - Uskoković-Marković, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2256
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey
T1  - Ocena bioaktivnih efekata flavonoida hesperidina - pregled podataka iz novije literature
VL  - 71
IS  - 1
SP  - 60
EP  - 65
DO  - 10.2298/VSP1401060K
ER  - 

@article{
author = "Kuntić, Vesna and Brborić, Jasmina and Holclajtner-Antunović, Ivanka and Uskoković-Marković, Snežana",
year = "2014",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey, Ocena bioaktivnih efekata flavonoida hesperidina - pregled podataka iz novije literature",
volume = "71",
number = "1",
pages = "60-65",
doi = "10.2298/VSP1401060K"
}

Kuntić, V., Brborić, J., Holclajtner-Antunović, I.,& Uskoković-Marković, S.. (2014). Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(1), 60-65.
https://doi.org/10.2298/VSP1401060K

Kuntić V, Brborić J, Holclajtner-Antunović I, Uskoković-Marković S. Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey. in Vojnosanitetski pregled. 2014;71(1):60-65.
doi:10.2298/VSP1401060K .

Kuntić, Vesna, Brborić, Jasmina, Holclajtner-Antunović, Ivanka, Uskoković-Marković, Snežana, "Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey" in Vojnosanitetski pregled, 71, no. 1 (2014):60-65,
https://doi.org/10.2298/VSP1401060K . .

TY  - CONF
AU  - Savić, Jelena
AU  - Brborić, Jasmina
AU  - Dilber, Sanda
AU  - Vladimirov, Sote
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5362
AB  - Although non-steroidal anti-inflammatory agents (NSAID) are numerous, broad used and can be procured as OTC drugs, search for new non-steroidal NSAID is continuing. Main motive is to find compound which selectively inhibits inducible form of enzyme cyclooxygenase (COX-2), but would have at least 10 times less effect on constitutive form (COX-1). If this selectivity concept is achieved, adverse effect on gastric mucosa would be avoided [1]. According to current docking studies, a compound is considered selective if it can maintain interactions in hydrophilic side pocket, so called P3 region in the active site of COX-2 [2]. The aim of this study was to determine the impact of substitution of one or both phenyl rings in 3-hydroxy-3,3-diphenylpropanoic acid with some simple substituents on selectivity towards COX-2 inhibition. Molecular docking calculations were performed using Autodock v4.0.1 into the 3D structure of the catalytic site of COX-2 enzyme (pdb code: 1cx2). Structure of each compound was generated using the ChemOffice v7.0 Ultra software package and have been MM2 optimized. Each docking experiment consisted of 100 docking runs with 150 individuals and 500,000 energy evaluations. The structures were incorporated into 40x40x40 grid points receptor pocket, which was centered to the position of ibuprofen in crystallographic structure of the complex. Ibuprofen was used as a reference compound because of its structure similarity to tested compounds. All of the compounds have lower binding energies than ibuprofen (Fig. 1) and all of these compounds have the right structure which enables penetration into P3 region in the COX-2. Compound containing dimethylamino group penetrates deepest into this region indicating the best selectivity ratio of all tested compounds.
PB  - Serbian Chemical Society
C3  - ICOSECS 8, 8th International Conference of the Chemical Societies of the South-East European Countries, BOOK OF ABSTRACTS, Belgrade, Serbia, June 27-29
T1  - Docking studies of 3-hydroxy-3-arylpropionic acids as potentially selective COX-2 inhibitors
SP  - 101
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5362
ER  - 

@conference{
author = "Savić, Jelena and Brborić, Jasmina and Dilber, Sanda and Vladimirov, Sote",
year = "2013",
abstract = "Although non-steroidal anti-inflammatory agents (NSAID) are numerous, broad used and can be procured as OTC drugs, search for new non-steroidal NSAID is continuing. Main motive is to find compound which selectively inhibits inducible form of enzyme cyclooxygenase (COX-2), but would have at least 10 times less effect on constitutive form (COX-1). If this selectivity concept is achieved, adverse effect on gastric mucosa would be avoided [1]. According to current docking studies, a compound is considered selective if it can maintain interactions in hydrophilic side pocket, so called P3 region in the active site of COX-2 [2]. The aim of this study was to determine the impact of substitution of one or both phenyl rings in 3-hydroxy-3,3-diphenylpropanoic acid with some simple substituents on selectivity towards COX-2 inhibition. Molecular docking calculations were performed using Autodock v4.0.1 into the 3D structure of the catalytic site of COX-2 enzyme (pdb code: 1cx2). Structure of each compound was generated using the ChemOffice v7.0 Ultra software package and have been MM2 optimized. Each docking experiment consisted of 100 docking runs with 150 individuals and 500,000 energy evaluations. The structures were incorporated into 40x40x40 grid points receptor pocket, which was centered to the position of ibuprofen in crystallographic structure of the complex. Ibuprofen was used as a reference compound because of its structure similarity to tested compounds. All of the compounds have lower binding energies than ibuprofen (Fig. 1) and all of these compounds have the right structure which enables penetration into P3 region in the COX-2. Compound containing dimethylamino group penetrates deepest into this region indicating the best selectivity ratio of all tested compounds.",
publisher = "Serbian Chemical Society",
journal = "ICOSECS 8, 8th International Conference of the Chemical Societies of the South-East European Countries, BOOK OF ABSTRACTS, Belgrade, Serbia, June 27-29",
title = "Docking studies of 3-hydroxy-3-arylpropionic acids as potentially selective COX-2 inhibitors",
pages = "101-101",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5362"
}

Savić, J., Brborić, J., Dilber, S.,& Vladimirov, S.. (2013). Docking studies of 3-hydroxy-3-arylpropionic acids as potentially selective COX-2 inhibitors. in ICOSECS 8, 8th International Conference of the Chemical Societies of the South-East European Countries, BOOK OF ABSTRACTS, Belgrade, Serbia, June 27-29
Serbian Chemical Society., 101-101.
https://hdl.handle.net/21.15107/rcub_farfar_5362

Savić J, Brborić J, Dilber S, Vladimirov S. Docking studies of 3-hydroxy-3-arylpropionic acids as potentially selective COX-2 inhibitors. in ICOSECS 8, 8th International Conference of the Chemical Societies of the South-East European Countries, BOOK OF ABSTRACTS, Belgrade, Serbia, June 27-29. 2013;:101-101.
https://hdl.handle.net/21.15107/rcub_farfar_5362 .

Savić, Jelena, Brborić, Jasmina, Dilber, Sanda, Vladimirov, Sote, "Docking studies of 3-hydroxy-3-arylpropionic acids as potentially selective COX-2 inhibitors" in ICOSECS 8, 8th International Conference of the Chemical Societies of the South-East European Countries, BOOK OF ABSTRACTS, Belgrade, Serbia, June 27-29 (2013):101-101,
https://hdl.handle.net/21.15107/rcub_farfar_5362 .

TY  - JOUR
AU  - Brborić, Jasmina
AU  - Jovanović, M.S.
AU  - Vranješ-Đurić, Sanja D.
AU  - Čudina, Olivera
AU  - Marković, Bojan
AU  - Vladimirov, Sote
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1932
AB  - The partition coefficients (log P) of theoretically possible alkyliodinated iminodiacetic acid (IDA) derivatives and commercial IDA derivatives were calculated using two computer programs: ChemSketch Log P and ChemOffice Ultra. Newly synthesized ligands (DIETHYLIODIDA and DIISOPROPYLIODIDA) with the highest calculated log P were labeled with technetium-99m. The biodistribution and the influence of bilirubin on their biokinetics were investigated in rats and compared to corresponding results for commercial Tc-99m-BROMIDA. Log P of Tc-99m-complexes of synthesized ligands were determined experimentally as well as the protein binding. In comparison to Tc-99m-BROMIDA, Tc-99m-DIETHYLIODIDA has: (a) better biliary excretion (2.76 +/- 0.15%ID/g versus 1.83 +/- 0.10%ID/g); (b) faster hepatic clearance (2.90 +/- 0.21%ID/g versus 7.47 +/- 0.70%ID/g) and decreased biliary excretion (for 14% versus 22%) in conditions of hyperbilirubinemia after 15 min. It is proved that Tc-99m-DIISOPROPYLIODIDA has a prolonged hepatic transit time and decreased biliary excretion.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Applied Radiation and Isotopes
T1  - The effect of lipophilicity on the hepatobiliary properties of iminodiacetic acid derivatives in the conditions of hyperbilirubinemia
VL  - 74
SP  - 31
EP  - 35
DO  - 10.1016/j.apradiso.2012.12.014
ER  - 

@article{
author = "Brborić, Jasmina and Jovanović, M.S. and Vranješ-Đurić, Sanja D. and Čudina, Olivera and Marković, Bojan and Vladimirov, Sote",
year = "2013",
abstract = "The partition coefficients (log P) of theoretically possible alkyliodinated iminodiacetic acid (IDA) derivatives and commercial IDA derivatives were calculated using two computer programs: ChemSketch Log P and ChemOffice Ultra. Newly synthesized ligands (DIETHYLIODIDA and DIISOPROPYLIODIDA) with the highest calculated log P were labeled with technetium-99m. The biodistribution and the influence of bilirubin on their biokinetics were investigated in rats and compared to corresponding results for commercial Tc-99m-BROMIDA. Log P of Tc-99m-complexes of synthesized ligands were determined experimentally as well as the protein binding. In comparison to Tc-99m-BROMIDA, Tc-99m-DIETHYLIODIDA has: (a) better biliary excretion (2.76 +/- 0.15%ID/g versus 1.83 +/- 0.10%ID/g); (b) faster hepatic clearance (2.90 +/- 0.21%ID/g versus 7.47 +/- 0.70%ID/g) and decreased biliary excretion (for 14% versus 22%) in conditions of hyperbilirubinemia after 15 min. It is proved that Tc-99m-DIISOPROPYLIODIDA has a prolonged hepatic transit time and decreased biliary excretion.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Applied Radiation and Isotopes",
title = "The effect of lipophilicity on the hepatobiliary properties of iminodiacetic acid derivatives in the conditions of hyperbilirubinemia",
volume = "74",
pages = "31-35",
doi = "10.1016/j.apradiso.2012.12.014"
}

Brborić, J., Jovanović, M.S., Vranješ-Đurić, S. D., Čudina, O., Marković, B.,& Vladimirov, S.. (2013). The effect of lipophilicity on the hepatobiliary properties of iminodiacetic acid derivatives in the conditions of hyperbilirubinemia. in Applied Radiation and Isotopes
Pergamon-Elsevier Science Ltd, Oxford., 74, 31-35.
https://doi.org/10.1016/j.apradiso.2012.12.014

Brborić J, Jovanović M, Vranješ-Đurić SD, Čudina O, Marković B, Vladimirov S. The effect of lipophilicity on the hepatobiliary properties of iminodiacetic acid derivatives in the conditions of hyperbilirubinemia. in Applied Radiation and Isotopes. 2013;74:31-35.
doi:10.1016/j.apradiso.2012.12.014 .

Brborić, Jasmina, Jovanović, M.S., Vranješ-Đurić, Sanja D., Čudina, Olivera, Marković, Bojan, Vladimirov, Sote, "The effect of lipophilicity on the hepatobiliary properties of iminodiacetic acid derivatives in the conditions of hyperbilirubinemia" in Applied Radiation and Isotopes, 74 (2013):31-35,
https://doi.org/10.1016/j.apradiso.2012.12.014 . .

TY  - JOUR
AU  - Smajić, Miralem
AU  - Vujić, Zorica
AU  - Mulavdić, Nedzad
AU  - Brborić, Jasmina
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1930
AB  - An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol
VL  - 76
IS  - 7-8
SP  - 419
EP  - 425
DO  - 10.1007/s10337-013-2388-8
ER  - 

@article{
author = "Smajić, Miralem and Vujić, Zorica and Mulavdić, Nedzad and Brborić, Jasmina",
year = "2013",
abstract = "An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol",
volume = "76",
number = "7-8",
pages = "419-425",
doi = "10.1007/s10337-013-2388-8"
}

Smajić, M., Vujić, Z., Mulavdić, N.,& Brborić, J.. (2013). An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia
Springer Heidelberg, Heidelberg., 76(7-8), 419-425.
https://doi.org/10.1007/s10337-013-2388-8

Smajić M, Vujić Z, Mulavdić N, Brborić J. An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia. 2013;76(7-8):419-425.
doi:10.1007/s10337-013-2388-8 .

Smajić, Miralem, Vujić, Zorica, Mulavdić, Nedzad, Brborić, Jasmina, "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol" in Chromatographia, 76, no. 7-8 (2013):419-425,
https://doi.org/10.1007/s10337-013-2388-8 . .

TY  - JOUR
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Brborić, Jasmina
AU  - Kuntić, Vesna
AU  - Uskoković-Marković, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1928
AB  - The aim of the present study was to optimize a chromatographic method for the analysis of atorvastatin (acid and lactone forms), ortho- and para-hydroxyatorvastatin by using an experimental design approach. Optimization experiments were conducted through a process of screening and optimization. The purpose of a screening design is to identify the factors that have significant effects on the selected chromatographic responses, and for this purpose a full 23 factorial design was used. The location of the true optimum was established by applying Derringer's desirability function, which provides simultaneously optimization of all seven responses. The ranges of the independent variables used for the optimization were content of acetonitrile in mobile phase (60-70%), temperature of column (30-40 C degrees) and flow rate (0.8-1.2 mL min(-1)). The influences of these independent variables were evaluated for the output responses: retention time of first peak (p-hydroxyatorvastatin) and of last peak (atorvastatin, lactone form), symmetries of all four peaks and relative retention time of p-hydroxyatorvastatin. The primary goal of this investigation was establishing a new simple and sensitive method that could be used in analysis of biological samples. The method was validated and successfully applied for determination of atorvastatin (acid and lactone forms) and its metabolites in plasma.
PB  - MDPI, Basel
T2  - Molecules
T1  - An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma
VL  - 18
IS  - 3
SP  - 2469
EP  - 2482
DO  - 10.3390/molecules18032469
ER  - 

@article{
author = "Crevar-Sakač, Milkica and Vujić, Zorica and Brborić, Jasmina and Kuntić, Vesna and Uskoković-Marković, Snežana",
year = "2013",
abstract = "The aim of the present study was to optimize a chromatographic method for the analysis of atorvastatin (acid and lactone forms), ortho- and para-hydroxyatorvastatin by using an experimental design approach. Optimization experiments were conducted through a process of screening and optimization. The purpose of a screening design is to identify the factors that have significant effects on the selected chromatographic responses, and for this purpose a full 23 factorial design was used. The location of the true optimum was established by applying Derringer's desirability function, which provides simultaneously optimization of all seven responses. The ranges of the independent variables used for the optimization were content of acetonitrile in mobile phase (60-70%), temperature of column (30-40 C degrees) and flow rate (0.8-1.2 mL min(-1)). The influences of these independent variables were evaluated for the output responses: retention time of first peak (p-hydroxyatorvastatin) and of last peak (atorvastatin, lactone form), symmetries of all four peaks and relative retention time of p-hydroxyatorvastatin. The primary goal of this investigation was establishing a new simple and sensitive method that could be used in analysis of biological samples. The method was validated and successfully applied for determination of atorvastatin (acid and lactone forms) and its metabolites in plasma.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma",
volume = "18",
number = "3",
pages = "2469-2482",
doi = "10.3390/molecules18032469"
}

Crevar-Sakač, M., Vujić, Z., Brborić, J., Kuntić, V.,& Uskoković-Marković, S.. (2013). An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma. in Molecules
MDPI, Basel., 18(3), 2469-2482.
https://doi.org/10.3390/molecules18032469

Crevar-Sakač M, Vujić Z, Brborić J, Kuntić V, Uskoković-Marković S. An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma. in Molecules. 2013;18(3):2469-2482.
doi:10.3390/molecules18032469 .

Crevar-Sakač, Milkica, Vujić, Zorica, Brborić, Jasmina, Kuntić, Vesna, Uskoković-Marković, Snežana, "An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma" in Molecules, 18, no. 3 (2013):2469-2482,
https://doi.org/10.3390/molecules18032469 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Stanković, Miroslava
AU  - Vujić, Zorica
AU  - Brborić, Jasmina
AU  - Uskoković-Marković, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1910
AB  - To protect organisms from ionizing radiation (IR), and to reduce morbidity or mortality, various agents, called radioprotectors, have been utilized. Because radiation-induced cellular damage is attributed primarily to the harmful effects of free radicals, molecules with radical-scavenging properties are particularly promising as radioprotectors. Early development of such agents focused on thiol synthetic compounds, known as WR protectors, but only amifostine (WR-2721) has been used in clinical trials as an officially approved radioprotector. Besides thiol compounds, various compounds with different chemical structure were investigated, but an ideal radioprotector has not been found yet. Plants and natural products have been evaluated as promising sources of radioprotectors because of their low toxicity, although they exhibit an inferior protection level compared to synthetic thiol compounds. Active plant constituents seem to exert the radioprotection through antioxidant and free radical-scavenging activities. Our research established that plants containing polyphenolic compounds (raspberry, blueberry, strawberry, grape, etc.) exhibit antioxidative activities and protect genetic material from IR.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Radioprotectors - the Evergreen Topic
VL  - 10
IS  - 10
SP  - 1791
EP  - 1803
DO  - 10.1002/cbdv.201300054
ER  - 

@article{
author = "Kuntić, Vesna and Stanković, Miroslava and Vujić, Zorica and Brborić, Jasmina and Uskoković-Marković, Snežana",
year = "2013",
abstract = "To protect organisms from ionizing radiation (IR), and to reduce morbidity or mortality, various agents, called radioprotectors, have been utilized. Because radiation-induced cellular damage is attributed primarily to the harmful effects of free radicals, molecules with radical-scavenging properties are particularly promising as radioprotectors. Early development of such agents focused on thiol synthetic compounds, known as WR protectors, but only amifostine (WR-2721) has been used in clinical trials as an officially approved radioprotector. Besides thiol compounds, various compounds with different chemical structure were investigated, but an ideal radioprotector has not been found yet. Plants and natural products have been evaluated as promising sources of radioprotectors because of their low toxicity, although they exhibit an inferior protection level compared to synthetic thiol compounds. Active plant constituents seem to exert the radioprotection through antioxidant and free radical-scavenging activities. Our research established that plants containing polyphenolic compounds (raspberry, blueberry, strawberry, grape, etc.) exhibit antioxidative activities and protect genetic material from IR.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Radioprotectors - the Evergreen Topic",
volume = "10",
number = "10",
pages = "1791-1803",
doi = "10.1002/cbdv.201300054"
}

Kuntić, V., Stanković, M., Vujić, Z., Brborić, J.,& Uskoković-Marković, S.. (2013). Radioprotectors - the Evergreen Topic. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 10(10), 1791-1803.
https://doi.org/10.1002/cbdv.201300054

Kuntić V, Stanković M, Vujić Z, Brborić J, Uskoković-Marković S. Radioprotectors - the Evergreen Topic. in Chemistry & Biodiversity. 2013;10(10):1791-1803.
doi:10.1002/cbdv.201300054 .

Kuntić, Vesna, Stanković, Miroslava, Vujić, Zorica, Brborić, Jasmina, Uskoković-Marković, Snežana, "Radioprotectors - the Evergreen Topic" in Chemistry & Biodiversity, 10, no. 10 (2013):1791-1803,
https://doi.org/10.1002/cbdv.201300054 . .