TY  - JOUR
AU  - Božić, Dragana
AU  - Bufan, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5603
AB  - Antibacterial vaccines play a central role in modern medicine by providing an effective approach to
combating infectious diseases caused by bacteria. The importance of these vaccines lies in their ability to
stimulate the immune system to recognise and neutralise bacterial pathogens, or exotoxins produced by them,
thereby preventing, or mitigating the severity of bacterial infections. The development and widespread use of
antibacterial vaccines have contributed significantly to reducing the global burden of diseases such as pneumonia,
meningitis, and sepsis.
Today, the global increase in vaccine-preventable diseases is a worrying trend that is closely linked to the
emergence and advocacy of anti-vaccination policies. According to the latest World Health Organisation report,
vaccination coverage in Serbia has declined over the past decade, jeopardising the collective immunity and led to
recent outbreaks of vaccine-preventable diseases such as whooping cough and measles. Understanding the
significance of antibacterial vaccines underscores their importance in promoting individual and community immunity,
which ultimately leads to a healthier population and the prevention of antibiotic resistance.
This paper summarises the main characteristics of the different types of antibacterial vaccines, such as whole cell
vaccines, subunit vaccines and toxoid vaccines, and provides an overview of the types of bacterial antigens
contained in vaccines available for mandatory immunisation (vaccines against tuberculosis, diphtheria, tetanus,
pertussis, Haemophilus influenzae and pneumoccus) or for non-mandatory immunisation (meningococcal vaccine,
typhoid vaccine, cholera vaccine).
AB  - Antibakterijske vakcine igraju glavnu ulogu u savremenoj medicini obezbeđujući efikasan pristup u borbi protiv
zaraznih bolesti izazvanih bakterijama. Važnost ovih vakcina leži u njihovoj sposobnosti da stimulišu imunski sistem
da prepozna i neutrališe bakterijske patogene, ili egzotoksine koje oni proizvode, čime sprečavaju ili ublažavaju
ozbiljnost bakterijskih infekcija. Razvoj i široka upotreba antibakterijskih vakcina značajno su doprineli smanjenju
globalnog tereta bolesti kao što su pneumonija, meningitis i sepsa.
Danas je globalni porast bolesti koje se mogu sprečiti vakcinama zabrinjavajući trend koji je usko povezan sa
pojavom i zagovaranjem politike protiv vakcinacije. Prema poslednjem izveštaju Svetske zdravstvene organizacije,
pokrivenost vakcinacijom u Srbiji je opala tokom protekle decenije, što je ugrozilo kolektivni imunitet i dovelo do
nedavnih izbijanja bolesti koje se mogu sprečiti vakcinom, poput velikog kašlja i malih boginja. Razumevanje
značaja antibakterijskih vakcina naglašava njihov značaj u promovisanju imuniteta pojedinca i zajednice, što na
kraju dovodi do zdravije populacije i prevencije rezistencije na antibiotike.
Ovaj rad sumira glavne karakteristike različitih tipova antibakterijskih vakcina, kao što su celo ćelijske vakcine,
podjedinične vakcine i toksoidne vakcine, i daje pregled tipova bakterijskih antigena sadržanih u vakcinama
dostupnim za obaveznu imunizaciju (vakcine protiv tuberkuloze, difterije, tetanusa, pertusisa, Haemophilus
influenzae i pneumoka) ili za neobaveznu imunizaciju (vakcina protiv meningokoka, tifusa i kolere).
PB  - Udruženja za preventivnu pedijatriju Srbije
T2  - Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije
T1  - An overview of current vacccines for the prophylaxis of bacterical infections Pres
T1  - Pregled aktuelnih vakcina za profilaksu bakterijskih infekcija
VL  - 10
IS  - 1-2
SP  - 19
EP  - 26
DO  - 10.46793/PP240214004B
DO  - 10.46793/PP240214004B
ER  - 

@article{
author = "Božić, Dragana and Bufan, Biljana",
year = "2024",
abstract = "Antibacterial vaccines play a central role in modern medicine by providing an effective approach to
combating infectious diseases caused by bacteria. The importance of these vaccines lies in their ability to
stimulate the immune system to recognise and neutralise bacterial pathogens, or exotoxins produced by them,
thereby preventing, or mitigating the severity of bacterial infections. The development and widespread use of
antibacterial vaccines have contributed significantly to reducing the global burden of diseases such as pneumonia,
meningitis, and sepsis.
Today, the global increase in vaccine-preventable diseases is a worrying trend that is closely linked to the
emergence and advocacy of anti-vaccination policies. According to the latest World Health Organisation report,
vaccination coverage in Serbia has declined over the past decade, jeopardising the collective immunity and led to
recent outbreaks of vaccine-preventable diseases such as whooping cough and measles. Understanding the
significance of antibacterial vaccines underscores their importance in promoting individual and community immunity,
which ultimately leads to a healthier population and the prevention of antibiotic resistance.
This paper summarises the main characteristics of the different types of antibacterial vaccines, such as whole cell
vaccines, subunit vaccines and toxoid vaccines, and provides an overview of the types of bacterial antigens
contained in vaccines available for mandatory immunisation (vaccines against tuberculosis, diphtheria, tetanus,
pertussis, Haemophilus influenzae and pneumoccus) or for non-mandatory immunisation (meningococcal vaccine,
typhoid vaccine, cholera vaccine)., Antibakterijske vakcine igraju glavnu ulogu u savremenoj medicini obezbeđujući efikasan pristup u borbi protiv
zaraznih bolesti izazvanih bakterijama. Važnost ovih vakcina leži u njihovoj sposobnosti da stimulišu imunski sistem
da prepozna i neutrališe bakterijske patogene, ili egzotoksine koje oni proizvode, čime sprečavaju ili ublažavaju
ozbiljnost bakterijskih infekcija. Razvoj i široka upotreba antibakterijskih vakcina značajno su doprineli smanjenju
globalnog tereta bolesti kao što su pneumonija, meningitis i sepsa.
Danas je globalni porast bolesti koje se mogu sprečiti vakcinama zabrinjavajući trend koji je usko povezan sa
pojavom i zagovaranjem politike protiv vakcinacije. Prema poslednjem izveštaju Svetske zdravstvene organizacije,
pokrivenost vakcinacijom u Srbiji je opala tokom protekle decenije, što je ugrozilo kolektivni imunitet i dovelo do
nedavnih izbijanja bolesti koje se mogu sprečiti vakcinom, poput velikog kašlja i malih boginja. Razumevanje
značaja antibakterijskih vakcina naglašava njihov značaj u promovisanju imuniteta pojedinca i zajednice, što na
kraju dovodi do zdravije populacije i prevencije rezistencije na antibiotike.
Ovaj rad sumira glavne karakteristike različitih tipova antibakterijskih vakcina, kao što su celo ćelijske vakcine,
podjedinične vakcine i toksoidne vakcine, i daje pregled tipova bakterijskih antigena sadržanih u vakcinama
dostupnim za obaveznu imunizaciju (vakcine protiv tuberkuloze, difterije, tetanusa, pertusisa, Haemophilus
influenzae i pneumoka) ili za neobaveznu imunizaciju (vakcina protiv meningokoka, tifusa i kolere).",
publisher = "Udruženja za preventivnu pedijatriju Srbije",
journal = "Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije",
title = "An overview of current vacccines for the prophylaxis of bacterical infections Pres, Pregled aktuelnih vakcina za profilaksu bakterijskih infekcija",
volume = "10",
number = "1-2",
pages = "19-26",
doi = "10.46793/PP240214004B, 10.46793/PP240214004B"
}

Božić, D.,& Bufan, B.. (2024). An overview of current vacccines for the prophylaxis of bacterical infections Pres. in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije
Udruženja za preventivnu pedijatriju Srbije., 10(1-2), 19-26.
https://doi.org/10.46793/PP240214004B

Božić D, Bufan B. An overview of current vacccines for the prophylaxis of bacterical infections Pres. in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije. 2024;10(1-2):19-26.
doi:10.46793/PP240214004B .

Božić, Dragana, Bufan, Biljana, "An overview of current vacccines for the prophylaxis of bacterical infections Pres" in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije, 10, no. 1-2 (2024):19-26,
https://doi.org/10.46793/PP240214004B . .

TY  - JOUR
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Antić-Stanković, Jelena
AU  - Arsenović-Ranin, Nevena
AU  - Bufan, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5582
AB  - The normal human microbiota, formerly called the "microbial flora," consists of bacteria,
fungi, viruses, and parasites that colonise the skin and mucous membranes of the respiratory,
gastrointestinal, and genitourinary tracts. The number and diversity of microorganisms varies
between different body niches and is greatest in the intestinal tract. The microbiota contributes to
the homeostasis of the human organism by preventing colonisation by pathogenic
microorganisms, participating in digestive processes and metabolism, and regulating immune
functions.
Various environmental and genetic factors can lead to an imbalance in the human
microbiota, called dysbiosis, which can affect human health. Dysbiosis is usually the result of
decreased microbial diversity and a lower number of saprophytic microorganisms, followed by
an overgrowth of opportunistic species. The most common diseases directly related to intestinal
dysbiosis are antibiotic-associated diarrhoea and pseudomembranous colitis, both of which are
associated with the excessive growth of harmful bacteria and Clostridioides difficile following
broad-spectrum antibiotic therapy.
Dysbiosis is associated with various health conditions or diseases such as acne, psoriasis,
eczema, chronic obstructive pulmonary disease, inflammatory bowel disease, obesity, metabolic
syndrome, type 2 diabetes, autoimmune diseases and allergies, neurological diseases such as
Parkinson's disease, Alzheimer's disease, epilepsy and stroke, depression, anxiety, infertility,
preterm birth, and malignancies.
AB  - Normalna ljudska mikrobiota, koja se ranije nazivala „mikroflora“, sastoji se od bakterija,
gljivica, virusa i parazita koji kolonizuju kožu i sluzokožu respiratornog, gastrointestinalnog i
genitourinarnog trakta. Broj i raznovrsnost mikroorganizama variraju između različitih telesnih
niša i najveći su u crevnom traktu. Mikrobiota doprinosi homeostazi ljudskog organizma tako što
sprečava kolonizaciju patogenim mikroorganizmima, učestvuje u procesima varenja i
metabolizma i reguliše imunološke funkcije.
Disbioza je stanje u kome dolazi do neravnoteže sastava mikrobiote usled uticaja različitih
egzogenih ili endogenih faktora, što može uticati na ljudsko zdravlje. Ona je najčešće rezultat
smanjene raznovrsnosti mikroorganizama i manjeg broja saprofitnih bakterija, što je praćeno
prekomernim rastom potencijalno štetnih vrsta. Najčešće bolesti koje su direktno povezane sa
crevnom disbiozom su dijareja povezana sa primenom antibiotika i pseudomembranozni kolitis,
a obe nastaju kao posledica prekomernog rasta štetnih bakterija i Clostridioides difficile nakon
terapije antibioticima širokog spektra.
Disbioza je povezana sa različitim zdravstvenim stanjima ili bolestima kao što su akne,
psorijaza, ekcem, hronična opstruktivna bolest pluća, inflamatorna bolest creva, gojaznost,
metabolički sindrom, dijabetes tipa 2, autoimunske bolesti i alergije, neurološke bolesti kao što
su Parkinsonova bolest, Alchajmerova demencija, epilepsija i moždani udar, depresija,
anksioznost, neplodnost, prevremeni porođaj i maligni tumori.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
PB  - Beograd : Univerzitet u Beogradu - Farmaceutski fakultet
T2  - Arhiv za farmaciju
T1  - Normal human microbiota and dysbiosis - implications for health and disease
T1  - Normalna ljudska mikrobiota i disbioza - implikacije po zdravlje i bolest
VL  - 74
IS  - 1
SP  - 1
EP  - 22
DO  - 10.5937/arhfarm74-46612
ER  - 

@article{
author = "Božić, Dragana and Milenković, Marina and Antić-Stanković, Jelena and Arsenović-Ranin, Nevena and Bufan, Biljana",
year = "2024",
abstract = "The normal human microbiota, formerly called the "microbial flora," consists of bacteria,
fungi, viruses, and parasites that colonise the skin and mucous membranes of the respiratory,
gastrointestinal, and genitourinary tracts. The number and diversity of microorganisms varies
between different body niches and is greatest in the intestinal tract. The microbiota contributes to
the homeostasis of the human organism by preventing colonisation by pathogenic
microorganisms, participating in digestive processes and metabolism, and regulating immune
functions.
Various environmental and genetic factors can lead to an imbalance in the human
microbiota, called dysbiosis, which can affect human health. Dysbiosis is usually the result of
decreased microbial diversity and a lower number of saprophytic microorganisms, followed by
an overgrowth of opportunistic species. The most common diseases directly related to intestinal
dysbiosis are antibiotic-associated diarrhoea and pseudomembranous colitis, both of which are
associated with the excessive growth of harmful bacteria and Clostridioides difficile following
broad-spectrum antibiotic therapy.
Dysbiosis is associated with various health conditions or diseases such as acne, psoriasis,
eczema, chronic obstructive pulmonary disease, inflammatory bowel disease, obesity, metabolic
syndrome, type 2 diabetes, autoimmune diseases and allergies, neurological diseases such as
Parkinson's disease, Alzheimer's disease, epilepsy and stroke, depression, anxiety, infertility,
preterm birth, and malignancies., Normalna ljudska mikrobiota, koja se ranije nazivala „mikroflora“, sastoji se od bakterija,
gljivica, virusa i parazita koji kolonizuju kožu i sluzokožu respiratornog, gastrointestinalnog i
genitourinarnog trakta. Broj i raznovrsnost mikroorganizama variraju između različitih telesnih
niša i najveći su u crevnom traktu. Mikrobiota doprinosi homeostazi ljudskog organizma tako što
sprečava kolonizaciju patogenim mikroorganizmima, učestvuje u procesima varenja i
metabolizma i reguliše imunološke funkcije.
Disbioza je stanje u kome dolazi do neravnoteže sastava mikrobiote usled uticaja različitih
egzogenih ili endogenih faktora, što može uticati na ljudsko zdravlje. Ona je najčešće rezultat
smanjene raznovrsnosti mikroorganizama i manjeg broja saprofitnih bakterija, što je praćeno
prekomernim rastom potencijalno štetnih vrsta. Najčešće bolesti koje su direktno povezane sa
crevnom disbiozom su dijareja povezana sa primenom antibiotika i pseudomembranozni kolitis,
a obe nastaju kao posledica prekomernog rasta štetnih bakterija i Clostridioides difficile nakon
terapije antibioticima širokog spektra.
Disbioza je povezana sa različitim zdravstvenim stanjima ili bolestima kao što su akne,
psorijaza, ekcem, hronična opstruktivna bolest pluća, inflamatorna bolest creva, gojaznost,
metabolički sindrom, dijabetes tipa 2, autoimunske bolesti i alergije, neurološke bolesti kao što
su Parkinsonova bolest, Alchajmerova demencija, epilepsija i moždani udar, depresija,
anksioznost, neplodnost, prevremeni porođaj i maligni tumori.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije, Beograd : Univerzitet u Beogradu - Farmaceutski fakultet",
journal = "Arhiv za farmaciju",
title = "Normal human microbiota and dysbiosis - implications for health and disease, Normalna ljudska mikrobiota i disbioza - implikacije po zdravlje i bolest",
volume = "74",
number = "1",
pages = "1-22",
doi = "10.5937/arhfarm74-46612"
}

Božić, D., Milenković, M., Antić-Stanković, J., Arsenović-Ranin, N.,& Bufan, B.. (2024). Normal human microbiota and dysbiosis - implications for health and disease. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 74(1), 1-22.
https://doi.org/10.5937/arhfarm74-46612

Božić D, Milenković M, Antić-Stanković J, Arsenović-Ranin N, Bufan B. Normal human microbiota and dysbiosis - implications for health and disease. in Arhiv za farmaciju. 2024;74(1):1-22.
doi:10.5937/arhfarm74-46612 .

Božić, Dragana, Milenković, Marina, Antić-Stanković, Jelena, Arsenović-Ranin, Nevena, Bufan, Biljana, "Normal human microbiota and dysbiosis - implications for health and disease" in Arhiv za farmaciju, 74, no. 1 (2024):1-22,
https://doi.org/10.5937/arhfarm74-46612 . .

TY  - JOUR
AU  - Vukašinović, Mila
AU  - Pantelić, Ivana
AU  - Savić, Sanela
AU  - Cekić, Nebojša
AU  - Vukašinović Sekulić, Maja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Tošić, Anđela
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5311
AB  - Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.
PB  - MDPI
T2  - Cosmetics
T1  - Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety
VL  - 10
IS  - 6
DO  - 10.3390/cosmetics10060162
ER  - 

@article{
author = "Vukašinović, Mila and Pantelić, Ivana and Savić, Sanela and Cekić, Nebojša and Vukašinović Sekulić, Maja and Antić-Stanković, Jelena and Božić, Dragana and Tošić, Anđela and Tamburić, Slobodanka and Savić, Snežana",
year = "2023",
abstract = "Bioactive peptides are promising cosmetic active ingredients that can improve skin health
and appearance. They exhibit a broad spectrum of activity, including anti-aging, antioxidant, an-
timicrobial, and anti-inflammatory effects. The aim of this study was to develop a safe, stable, and
efficacious environmentally friendly (“green”) emulsion using a milk protein hydrolysate as a model
active ingredient. Potential emulsions were formulated with biodegradable emollients, stabilized
with naturally derived mixed emulsifier, and prepared by cold process. They were evaluated for
rheological behavior (continuous rotation and oscillation tests), physical stability (dynamic me-
chanical thermal analysis—DMTA test), and texture profiles, as well as cytotoxic, antioxidant, and
antimicrobial effects. Rheological characterization revealed shear-thinning flow behavior with yield
point from continuous rotation tests and predominantly elastic character from oscillation (amplitude
and frequency sweep) tests, with small structural change detected in the DMTA test. These results
implied satisfactory rheological properties and good stability. Texture analysis revealed acceptable
spreadability and substantivity of the emulsions. The protein hydrolysate showed antioxidant activity.
The developed emulsions showed low antibacterial activity against selected microorganisms, but
this was due to the action of preservatives, not peptides. All potential emulsions showed a desirable
safety profile. The results obtained provide the basis for the next stage of formulation development,
i.e., in vivo efficacy tests.",
publisher = "MDPI",
journal = "Cosmetics",
title = "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety",
volume = "10",
number = "6",
doi = "10.3390/cosmetics10060162"
}

Vukašinović, M., Pantelić, I., Savić, S., Cekić, N., Vukašinović Sekulić, M., Antić-Stanković, J., Božić, D., Tošić, A., Tamburić, S.,& Savić, S.. (2023). Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics
MDPI., 10(6).
https://doi.org/10.3390/cosmetics10060162

Vukašinović M, Pantelić I, Savić S, Cekić N, Vukašinović Sekulić M, Antić-Stanković J, Božić D, Tošić A, Tamburić S, Savić S. Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety. in Cosmetics. 2023;10(6).
doi:10.3390/cosmetics10060162 .

Vukašinović, Mila, Pantelić, Ivana, Savić, Sanela, Cekić, Nebojša, Vukašinović Sekulić, Maja, Antić-Stanković, Jelena, Božić, Dragana, Tošić, Anđela, Tamburić, Slobodanka, Savić, Snežana, "Development of a “Green” Emulsion with a Milk Protein Hydrolysate: An Evaluation of Rheology, Texture, In Vitro Bioactivity, and Safety" in Cosmetics, 10, no. 6 (2023),
https://doi.org/10.3390/cosmetics10060162 . .

TY  - CONF
AU  - Dabetić, Nevena
AU  - Todorović, Vanja
AU  - Božić, Dragana
AU  - Antić-Stanković, Jelena
AU  - Šobajić, Slađana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5140
AB  - Semenke predstavljaju najvredniji deo ploda vinove loze. Polifenolna jedinjenja se primarno
koncentrišu tamo, zbog čega semenke poseduju jaku antioksidativnu aktivnost. Zahvaljujući tome,
ekstrakti semenki mogu delovati kao antimikrobni agensi. Ekstrakcija bioaktivnih jedinjenja se
obično vrši pomoću isparljivih, organskih rastvarača. Poslednjih godina, zajedno sa povećanjem
svesti o značaju zaštite životne sredine, različiti „zeleni“ rastvarači su se pojavili kao alternativa
konvencionalnim.
Ova studija je imala za cilj ispitivanje razlika između semenki različitih sorti grožđa u pogledu
njihove antimikrobne aktivnosti prema Staphylococcus aureus. Takođe je procenjen afinitet
eutektičkog rastvarača za ekstrakciju polifenola iz semenki grožđa. Kao referentni rastvarač
korišćen je etanol.
Semenke su izolovane iz pet različitih sorti crvenog grožđa koje se široko gaje u Srbiji.
Ultrazvučna ekstrakcija izvedena je korišćenjem eutektičkog rastvarača (holin hlorid:limunska
kiselina, 2:1, 30 % vode; ChCit) i 70 % etanola. Ukupan sadržaj polifenola (eng. Total Phenolic
Content; TPC) je određen Folin-Ciocalteu metodom. Antimikrobna aktivnost uzoraka je ispitivana
je na standardnom soju Staphylococcus aureus ATCC 6538 bujon-mikrodilucionim testom.
TPC vrednosti su se kretale od 91,75 do 116,71 i od 84,79 do 135,5 mg GAE/g suve mase (za
ChCit i etanolne ekstrakte, respektivno). Semenke sorte Prokupac imale su najveći sadržaj ukupnih
polifenola, bez obzira na upotrebljeni rastvarač. Vrednosti minimalnih inhibitornih koncentracija
(MIK) su se kretale između 0,04 i 0,15 mg/ml. ChCit ekstrakti su bili slični ili čak efikasniji protiv
ove Gram-pozitivne bakterije u poređenju sa etanolnim ekstraktima. Ovo bi moglo biti delimično
objašnjeno antimikrobnom aktivnošću samog rastvarača, pošto je primećeno da ChCit inhibira rast
Staphylococcus aureus (MIK=0,3 mg/ml).
Imajući u vidu da su ekstrakti pokazali znatno jaču aktivnost u odnosu na rastvarač i da su
rezultati ispitivanja antimikrobne aktivnosti bili u značajnoj korelaciji sa sadržajem polifenola,
može se zaključiti da je sinergizam između prisutnih polifenola i ChCit rezultirao snažnom
antimikrobnom aktivnošću protiv Staphylococcus aureus u ekološki prihvatljivijim ekstraktima.
PB  - Srpsko udruženje za kliničku mikrobiologiju (SUKM)
C3  - SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata
T1  - Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa
SP  - 56
EP  - 56
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5140
ER  - 

@conference{
author = "Dabetić, Nevena and Todorović, Vanja and Božić, Dragana and Antić-Stanković, Jelena and Šobajić, Slađana",
year = "2023",
abstract = "Semenke predstavljaju najvredniji deo ploda vinove loze. Polifenolna jedinjenja se primarno
koncentrišu tamo, zbog čega semenke poseduju jaku antioksidativnu aktivnost. Zahvaljujući tome,
ekstrakti semenki mogu delovati kao antimikrobni agensi. Ekstrakcija bioaktivnih jedinjenja se
obično vrši pomoću isparljivih, organskih rastvarača. Poslednjih godina, zajedno sa povećanjem
svesti o značaju zaštite životne sredine, različiti „zeleni“ rastvarači su se pojavili kao alternativa
konvencionalnim.
Ova studija je imala za cilj ispitivanje razlika između semenki različitih sorti grožđa u pogledu
njihove antimikrobne aktivnosti prema Staphylococcus aureus. Takođe je procenjen afinitet
eutektičkog rastvarača za ekstrakciju polifenola iz semenki grožđa. Kao referentni rastvarač
korišćen je etanol.
Semenke su izolovane iz pet različitih sorti crvenog grožđa koje se široko gaje u Srbiji.
Ultrazvučna ekstrakcija izvedena je korišćenjem eutektičkog rastvarača (holin hlorid:limunska
kiselina, 2:1, 30 % vode; ChCit) i 70 % etanola. Ukupan sadržaj polifenola (eng. Total Phenolic
Content; TPC) je određen Folin-Ciocalteu metodom. Antimikrobna aktivnost uzoraka je ispitivana
je na standardnom soju Staphylococcus aureus ATCC 6538 bujon-mikrodilucionim testom.
TPC vrednosti su se kretale od 91,75 do 116,71 i od 84,79 do 135,5 mg GAE/g suve mase (za
ChCit i etanolne ekstrakte, respektivno). Semenke sorte Prokupac imale su najveći sadržaj ukupnih
polifenola, bez obzira na upotrebljeni rastvarač. Vrednosti minimalnih inhibitornih koncentracija
(MIK) su se kretale između 0,04 i 0,15 mg/ml. ChCit ekstrakti su bili slični ili čak efikasniji protiv
ove Gram-pozitivne bakterije u poređenju sa etanolnim ekstraktima. Ovo bi moglo biti delimično
objašnjeno antimikrobnom aktivnošću samog rastvarača, pošto je primećeno da ChCit inhibira rast
Staphylococcus aureus (MIK=0,3 mg/ml).
Imajući u vidu da su ekstrakti pokazali znatno jaču aktivnost u odnosu na rastvarač i da su
rezultati ispitivanja antimikrobne aktivnosti bili u značajnoj korelaciji sa sadržajem polifenola,
može se zaključiti da je sinergizam između prisutnih polifenola i ChCit rezultirao snažnom
antimikrobnom aktivnošću protiv Staphylococcus aureus u ekološki prihvatljivijim ekstraktima.",
publisher = "Srpsko udruženje za kliničku mikrobiologiju (SUKM)",
journal = "SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata",
title = "Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa",
pages = "56-56",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5140"
}

Dabetić, N., Todorović, V., Božić, D., Antić-Stanković, J.,& Šobajić, S.. (2023). Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa. in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata
Srpsko udruženje za kliničku mikrobiologiju (SUKM)., 56-56.
https://hdl.handle.net/21.15107/rcub_farfar_5140

Dabetić N, Todorović V, Božić D, Antić-Stanković J, Šobajić S. Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa. in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata. 2023;:56-56.
https://hdl.handle.net/21.15107/rcub_farfar_5140 .

Dabetić, Nevena, Todorović, Vanja, Božić, Dragana, Antić-Stanković, Jelena, Šobajić, Slađana, "Zelena ekstrakcija sa antimikrobnom aktivnošću protiv Staphylococcus aureus iz semenki grožđa" in SKKM 2023 -  1. srpski kongres kliničke mikrobiologije sa međunarodnim učešćem, 10-12 maj 2023, Beograd - Knjiga apstrakata (2023):56-56,
https://hdl.handle.net/21.15107/rcub_farfar_5140 .

TY  - JOUR
AU  - Božić, Dragana
AU  - Ćirković, Ivana
AU  - Milovanović, Jovica
AU  - Bufan, Biljana
AU  - Folić, Miljan
AU  - Savić Vujović, Katarina
AU  - Pavlović, Bojan
AU  - Jotić, Ana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5341
AB  - Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.
PB  - MDPI
T2  - Pharmaceuticals
T1  - In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections
VL  - 16
IS  - 11
DO  - 10.3390/ph16111604
ER  - 

@article{
author = "Božić, Dragana and Ćirković, Ivana and Milovanović, Jovica and Bufan, Biljana and Folić, Miljan and Savić Vujović, Katarina and Pavlović, Bojan and Jotić, Ana",
year = "2023",
abstract = "Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections",
volume = "16",
number = "11",
doi = "10.3390/ph16111604"
}

Božić, D., Ćirković, I., Milovanović, J., Bufan, B., Folić, M., Savić Vujović, K., Pavlović, B.,& Jotić, A.. (2023). In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections. in Pharmaceuticals
MDPI., 16(11).
https://doi.org/10.3390/ph16111604

Božić D, Ćirković I, Milovanović J, Bufan B, Folić M, Savić Vujović K, Pavlović B, Jotić A. In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections. in Pharmaceuticals. 2023;16(11).
doi:10.3390/ph16111604 .

Božić, Dragana, Ćirković, Ivana, Milovanović, Jovica, Bufan, Biljana, Folić, Miljan, Savić Vujović, Katarina, Pavlović, Bojan, Jotić, Ana, "In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections" in Pharmaceuticals, 16, no. 11 (2023),
https://doi.org/10.3390/ph16111604 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Božić, Dragana
AU  - Filipović, Mila
AU  - Lukić, Milica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5350
AB  - An important contribution to sustainability is research into the usability of ingredients from waste materials (1). Therefore, the aim of our work was to investigate the contribution of ethanol extracts from wheat, corn, ...
PB  - APGI – “Association de Pharmacie Galénique Industrielle”
C3  - 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
T1  - Contribution of ethanol extracts from wheat, corn and sunflower waste material to the properties and effects of cosmetic products
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5350
ER  - 

@conference{
author = "Ćirić, Ana and Božić, Dragana and Filipović, Mila and Lukić, Milica",
year = "2023",
abstract = "An important contribution to sustainability is research into the usability of ingredients from waste materials (1). Therefore, the aim of our work was to investigate the contribution of ethanol extracts from wheat, corn, ...",
publisher = "APGI – “Association de Pharmacie Galénique Industrielle”",
journal = "6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France",
title = "Contribution of ethanol extracts from wheat, corn and sunflower waste material to the properties and effects of cosmetic products",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5350"
}

Ćirić, A., Božić, D., Filipović, M.,& Lukić, M.. (2023). Contribution of ethanol extracts from wheat, corn and sunflower waste material to the properties and effects of cosmetic products. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France
APGI – “Association de Pharmacie Galénique Industrielle”., 48-48.
https://hdl.handle.net/21.15107/rcub_farfar_5350

Ćirić A, Božić D, Filipović M, Lukić M. Contribution of ethanol extracts from wheat, corn and sunflower waste material to the properties and effects of cosmetic products. in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France. 2023;:48-48.
https://hdl.handle.net/21.15107/rcub_farfar_5350 .

Ćirić, Ana, Božić, Dragana, Filipović, Mila, Lukić, Milica, "Contribution of ethanol extracts from wheat, corn and sunflower waste material to the properties and effects of cosmetic products" in 6th Symposium Skin and Formulation, 2-3 October, 2023, Nantes - France (2023):48-48,
https://hdl.handle.net/21.15107/rcub_farfar_5350 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan M.
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4919
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 

@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan M.",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}

Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J. M.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis
Oxford University Press., 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013

Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković JM. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .

Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan M., "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .

TY  - JOUR
AU  - Krstić, Sanja
AU  - Miljić, Milorad
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Pirković, Andrea
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5034
AB  - Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.
PB  - Elsevier Ltd
T2  - Food Chemistry Advances
T1  - Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity
VL  - 3
DO  - 10.1016/j.focha.2023.100395
ER  - 

@article{
author = "Krstić, Sanja and Miljić, Milorad and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Pirković, Andrea",
year = "2023",
abstract = "Methanolic pulp extracts from the four selected Serbian accessions of Cucurbita maxima were evaluated for phenol profile cytotoxic effects and antimicrobial activity. The results revealed that quinic acid and amentoflavone were the most abundant phenols. The extracts increased the viability of HTR-8 SV/Neo, JEG-3, JAR cells, with the most pronounced increase in the treatment with MAX 113 extract. Furthermore, in HeLa cells, the extracts showed a modest cytotoxic effect. The antimicrobial effects evaluation showed that out of four pumpkin extracts, MAX 117 could moderately suppress the growth of Staphylococcus aureus and Staphylococcus epidermidis (MIC=1000 µg/mL). The observed biological effects indicate the potential medicinal properties of these pumpkin extracts and contribute to the varietal selection of the most suitable accessions in national breeding programs as candidates for improving human health.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry Advances",
title = "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity",
volume = "3",
doi = "10.1016/j.focha.2023.100395"
}

Krstić, S., Miljić, M., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Pirković, A.. (2023). Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances
Elsevier Ltd., 3.
https://doi.org/10.1016/j.focha.2023.100395

Krstić S, Miljić M, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Pirković A. Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity. in Food Chemistry Advances. 2023;3.
doi:10.1016/j.focha.2023.100395 .

Krstić, Sanja, Miljić, Milorad, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Pirković, Andrea, "Pumpkin pulp extracts from a Serbian Cucurbita maxima Breeding Collection: Phenol profile and in vitro bioactivity" in Food Chemistry Advances, 3 (2023),
https://doi.org/10.1016/j.focha.2023.100395 . .

TY  - CONF
AU  - Božić, Dragana
AU  - Ilić, Tijana
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4616
AB  - In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - In vitro assessment of the prebiotic potential of selected plant extracts
SP  - 54
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4616
ER  - 

@conference{
author = "Božić, Dragana and Ilić, Tijana and Marčetić, Mirjana and Samardžić, Stevan and Zdunić, Gordana and Vidović, Bojana",
year = "2022",
abstract = "In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "In vitro assessment of the prebiotic potential of selected plant extracts",
pages = "54-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4616"
}

Božić, D., Ilić, T., Marčetić, M., Samardžić, S., Zdunić, G.,& Vidović, B.. (2022). In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616

Božić D, Ilić T, Marčetić M, Samardžić S, Zdunić G, Vidović B. In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

Božić, Dragana, Ilić, Tijana, Marčetić, Mirjana, Samardžić, Stevan, Zdunić, Gordana, Vidović, Bojana, "In vitro assessment of the prebiotic potential of selected plant extracts" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):54-54,
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

TY  - CONF
AU  - Ilić, Tijana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Božić, Dragana
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4617
AB  - Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4617
ER  - 

@conference{
author = "Ilić, Tijana and Samardžić, Stevan and Zdunić, Gordana and Božić, Dragana and Marčetić, Mirjana and Vidović, Bojana",
year = "2022",
abstract = "Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4617"
}

Ilić, T., Samardžić, S., Zdunić, G., Božić, D., Marčetić, M.,& Vidović, B.. (2022). Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617

Ilić T, Samardžić S, Zdunić G, Božić D, Marčetić M, Vidović B. Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

Ilić, Tijana, Samardžić, Stevan, Zdunić, Gordana, Božić, Dragana, Marčetić, Mirjana, Vidović, Bojana, "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):44-44,
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4610
AB  - The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae
SP  - 167
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4610
ER  - 

@conference{
author = "Marčetić, Mirjana and Vidović, Bojana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana",
year = "2022",
abstract = "The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4610"
}

Marčetić, M., Vidović, B., Samardžić, S., Ilić, T.,& Božić, D.. (2022). Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610

Marčetić M, Vidović B, Samardžić S, Ilić T, Božić D. Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

Marčetić, Mirjana, Vidović, Bojana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):167-167,
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4311
AB  - Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.
PB  - MDPI
T2  - Foods
T1  - Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits
VL  - 11
IS  - 20
DO  - 10.3390/foods11203289
ER  - 

@article{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2022",
abstract = "Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.",
publisher = "MDPI",
journal = "Foods",
title = "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits",
volume = "11",
number = "20",
doi = "10.3390/foods11203289"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2022). Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods
MDPI., 11(20).
https://doi.org/10.3390/foods11203289

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods. 2022;11(20).
doi:10.3390/foods11203289 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits" in Foods, 11, no. 20 (2022),
https://doi.org/10.3390/foods11203289 . .

TY  - JOUR
AU  - Janošević-Ležaić, Aleksandra
AU  - Pašti, Igor
AU  - Gledović, Ana
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Uskoković-Marković, Snežana
AU  - Ćirić-Marjanović, Gordana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4067
AB  - Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.
PB  - Elsevier Ltd
T2  - Synthetic Metals
T1  - Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities
VL  - 286
DO  - 10.1016/j.synthmet.2022.117048
ER  - 

@article{
author = "Janošević-Ležaić, Aleksandra and Pašti, Igor and Gledović, Ana and Antić-Stanković, Jelena and Božić, Dragana and Uskoković-Marković, Snežana and Ćirić-Marjanović, Gordana",
year = "2022",
abstract = "Novel electroactive PANI-GA micro/nanostructured functional copolymers/co-oligomers were synthesized by the oxidative copolymerization of aniline and gallic acid (GA) in an aqueous solution, using ammonium peroxydisulfate (APS) as an oxidant and different initial mole ratios of GA to aniline ([GA]/[aniline]=0.1, 0.25, 0.5 and 1.0). It was found that the yield, molecular structure, morphology, electrical conductivity, electrochemical behavior, antioxidant and antimicrobial properties of PANI-GA strongly depend on [GA]/[aniline] ratio. The highest conductivity (3.8 × 10–3 S cm􀀀 1) showed PANI-GA with dominant polyaniline (PANI) emeraldine salt segments and nanorod morphology, synthesized at [GA]/[aniline] = 0.1. FTIR spectroscopy confirmed the presence of covalently bonded GA and PANI-type structural segments in PANI-GA. The antioxidant activity of PANI-GA was explored by the spectrophotometric 2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and electrochemical test for superoxide anion radical, •O2􀀀 . All protonated PANI-GA showed good scavenging activity toward ABTS•+ and •O2􀀀 radicals, whereby the highest activity exhibited PANI-GA produced at [GA]/[aniline] = 1. The radical scavenging activity of protonated PANI-GA samples toward ABTS•+ was much higher than that of PANI. The antimicrobial properties of the PANI-GA against bacteria S. aureus and E. coli, as well as against fungus C. albicans were evaluated. All synthesized protonated PANI-GA were more effective as S. aureus growth inhibitors than pristine GA and PANI. Highest effectiveness against E. coli and C. albicans, higher than that of pristine GA and PANI, showed conductive PANI-GA synthesized at [GA]/[aniline]= 0.1. Based on the obtained experimental results the mechanism of GA and aniline oxidative copolymerization is proposed.",
publisher = "Elsevier Ltd",
journal = "Synthetic Metals",
title = "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities",
volume = "286",
doi = "10.1016/j.synthmet.2022.117048"
}

Janošević-Ležaić, A., Pašti, I., Gledović, A., Antić-Stanković, J., Božić, D., Uskoković-Marković, S.,& Ćirić-Marjanović, G.. (2022). Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals
Elsevier Ltd., 286.
https://doi.org/10.1016/j.synthmet.2022.117048

Janošević-Ležaić A, Pašti I, Gledović A, Antić-Stanković J, Božić D, Uskoković-Marković S, Ćirić-Marjanović G. Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities. in Synthetic Metals. 2022;286.
doi:10.1016/j.synthmet.2022.117048 .

Janošević-Ležaić, Aleksandra, Pašti, Igor, Gledović, Ana, Antić-Stanković, Jelena, Božić, Dragana, Uskoković-Marković, Snežana, Ćirić-Marjanović, Gordana, "Copolymerization of aniline and gallic acid: Novel electroactive materials with antioxidant and antimicrobial activities" in Synthetic Metals, 286 (2022),
https://doi.org/10.1016/j.synthmet.2022.117048 . .

TY  - JOUR
AU  - Tomanić, Dragana
AU  - Božin, Biljana
AU  - Kladar, Nebojša
AU  - Stanojević, Jovan
AU  - Čabarkapa, Ivana
AU  - Stilinović, Nebojša
AU  - Apić, Jelena
AU  - Božić, Dragana
AU  - Kovačević, Zorana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4267
AB  - Mastitis is considered to be one of the most important diseases of dairy cows in terms of health, production, and economy. Being the most common cause of antibiotic consumption in dairy cows, treatment of this disease is one of the biggest challenges in the veterinary profession as an increasing number of pathogens develop resistance to antibiotics used in the treatment. Therefore, new alternative approaches for limiting the use of antibiotics in livestock are required. For this reason, our study aimed to investigate prevalence of environmental mastitis associated bacterial strains, as well as the sensitivity of isolated strains to different antibiotics. Additionally, the therapeutic potential of three essential oils (EOs) was tested against bovine Serratia spp. and Proteus spp. mastitis pathogens, based on their chemical composition, as well as antibacterial potential. The study was carried out on 81 milk samples collected from dairy cows with mastitis. In order to determine prevalence of S. marcescens and P. mirabilis, microbiological isolation and identification were performed. Antimicrobial susceptibility testing was performed by disk diffusion method and the microdilution method was used to determine the antibacterial activity of selected EOs. In the oregano EO, a total of 23 compounds were detected, with carvacrol as a dominant component (78.94%). A total of 26 components were present in the EO of common thyme, where thymol was the most abundant compound (46.37%). Thymol also dominated (55.11%) the wild thyme EO. All tested EOs displayed antibacterial activity against all strains to different extents, while wild and common thyme EOs were the most effective. It could be concluded that the tested EOs represent promising therapeutic candidates for effective non-antibiotic treatment of mastitis.
PB  - MDPI
T2  - Antibiotics
T1  - Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils
VL  - 11
IS  - 8
DO  - 10.3390/antibiotics11081077
ER  - 

@article{
author = "Tomanić, Dragana and Božin, Biljana and Kladar, Nebojša and Stanojević, Jovan and Čabarkapa, Ivana and Stilinović, Nebojša and Apić, Jelena and Božić, Dragana and Kovačević, Zorana",
year = "2022",
abstract = "Mastitis is considered to be one of the most important diseases of dairy cows in terms of health, production, and economy. Being the most common cause of antibiotic consumption in dairy cows, treatment of this disease is one of the biggest challenges in the veterinary profession as an increasing number of pathogens develop resistance to antibiotics used in the treatment. Therefore, new alternative approaches for limiting the use of antibiotics in livestock are required. For this reason, our study aimed to investigate prevalence of environmental mastitis associated bacterial strains, as well as the sensitivity of isolated strains to different antibiotics. Additionally, the therapeutic potential of three essential oils (EOs) was tested against bovine Serratia spp. and Proteus spp. mastitis pathogens, based on their chemical composition, as well as antibacterial potential. The study was carried out on 81 milk samples collected from dairy cows with mastitis. In order to determine prevalence of S. marcescens and P. mirabilis, microbiological isolation and identification were performed. Antimicrobial susceptibility testing was performed by disk diffusion method and the microdilution method was used to determine the antibacterial activity of selected EOs. In the oregano EO, a total of 23 compounds were detected, with carvacrol as a dominant component (78.94%). A total of 26 components were present in the EO of common thyme, where thymol was the most abundant compound (46.37%). Thymol also dominated (55.11%) the wild thyme EO. All tested EOs displayed antibacterial activity against all strains to different extents, while wild and common thyme EOs were the most effective. It could be concluded that the tested EOs represent promising therapeutic candidates for effective non-antibiotic treatment of mastitis.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils",
volume = "11",
number = "8",
doi = "10.3390/antibiotics11081077"
}

Tomanić, D., Božin, B., Kladar, N., Stanojević, J., Čabarkapa, I., Stilinović, N., Apić, J., Božić, D.,& Kovačević, Z.. (2022). Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils. in Antibiotics
MDPI., 11(8).
https://doi.org/10.3390/antibiotics11081077

Tomanić D, Božin B, Kladar N, Stanojević J, Čabarkapa I, Stilinović N, Apić J, Božić D, Kovačević Z. Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils. in Antibiotics. 2022;11(8).
doi:10.3390/antibiotics11081077 .

Tomanić, Dragana, Božin, Biljana, Kladar, Nebojša, Stanojević, Jovan, Čabarkapa, Ivana, Stilinović, Nebojša, Apić, Jelena, Božić, Dragana, Kovačević, Zorana, "Environmental Bovine Mastitis Pathogens: Prevalence, Antimicrobial Susceptibility, and Sensitivity to Thymus vulgaris L., Thymus serpyllum L., and Origanum vulgare L. Essential Oils" in Antibiotics, 11, no. 8 (2022),
https://doi.org/10.3390/antibiotics11081077 . .

TY  - CONF
AU  - Ivković, Branka
AU  - Božić, Dragana
AU  - Kotur-Stevuljević, Jelena
AU  - Krajišnik, Danina
AU  - Vujić, Zorica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4598
AB  - The global market for antiseptics and disinfectants is growing rapidly, fueled by a
coronavirus pandemic and a possible outbreak of infectious diseases in the future. The
antimicrobial, anti-inflammatory and redox activity of chalcone (1,3-diaryl-2-propen-1-one)
is well documented in the literature. The aim of this study is screening of antimicrobial
activity of chalcones and their saturated derivatives as active pharmaceutical ingredients,
synthesized by Claisen-Schmidt condensation (1). The redox potential of the investigated
compounds was tested in the biological environment using spectrophotometric methods to
determine prooxidant/antioxidant parameters. Within preformulation studies solubility and
compatibility with excipients commonly used in liquid pharmaceutical dosage forms was
performed. All the tested chalcones and their saturated derivatives showed satisfactory
antimicrobial activity, but two saturated chalcones showed the best MIC (0.156 - 1.25 mM)
and were categorized as compounds with strong bacteriostatic activity (2). Solubility of the
chalcons with moderate antimicrobial activity and redox potential was higher than its
minimum bacteriostatic and bactericidal concentration in all the tested solvents (ethanol,
isopropyl alcohol and propylene glycol). Based on the chemical structure and predicted logP
values for saturated chalcones that show stronger antimicrobial activity, better water
solubility is expected. These data could be a starting point for formulations of antiseptics and
disinfectants with lower concentrations of alcohol-based solvents, as the synergism between
saturated chalcone and ethanol and isopropyl alcohol was previously shown. Considering the
lighter color of the saturated chalcone, better aesthetic acceptability of the developed
formulations is expected, since it will not stain the skin and objects during application.
AB  - Globalno tržište antiseptika i dezinfekcionih sredstava ubrzano raste podstaknuto
pandemijom koronavirusa i moguć im širenjem drugih zaraznih bolesti u buduć nosti.
Antimikrobna, antiinflamatorna i redoks aktivnost halkona (1,3-diaril-2-propen-1-ona) je
dobro dokumentovana u literaturi. Cilj ove studije bio je skrining antimikrobne aktivnosti
halkona i njihovih zasić enih derivata kao aktivnih farmaceutskih sastojaka, sintetisanih
Claisen‐Schmidt kondenzacijom (1). Redoks potencijal ispitivanih jedinjenja je ispitan u
biosredini primenom spektrofotometrijskih metoda za određivanje
prooksidantnih/antioksidativnih parametara. Sprovedena su i preformulaciona ispitivanja
rastvorljivosti i kompatibilnosti sa ekscipijensima uobičajeno korišćenim u tečnim
farmaceutskim oblicima. Svi testirani halkoni i njihovi zasić eni derivati su pokazali
zadovoljavajuć u antimikrobnu aktivnost, ali su dva zasić ena halkona pokazala najbolji MIC
(0,156 – 1,25 mM) i kategorisana su kao jedinjenja sa jakom bakteriostatskom aktivnošć u
(2). Rastvorljivost halkona sa umerenom antimikrobnom aktivnošću i redoks potencijalom
bili su viši od njegove minimalne bakteriostatske i baktericidne koncentracije u svim
ispitivanim rastvaračima (etanol, izopropil alkohol i propilenglikol). Na osnovu hemijske
strukture i predviđene logP vrednosti za zasićene halkone koji pokazuju bolju antimikrobnu
aktivnost, očekuje se i bolja rastvorljivost u vodi. Ovi podaci mogli bi da budu polazna osnova
za formulisanje antiseptika i dezinfekcionih sredstava sa nižim koncentracijama rastvarača
na bazi alkohola, uzimajući u obzir da je u prethodnom ispitivanju pokazan sinergizam
između zasićenog halkona i etanola, kao i izopropil alkohola. S obzirom na svetliju boju
zasićenog halkona, očekuje se i bolja estetska prihvatljivost razvijenih formulacija, jer neć e
bojiti kožu i predmete tokom nanošenja.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chalcone derivatives as potential antiseptics and disinfectants
T1  - Derivati halkona kao potentijalni antiseptici i dezinficijensi
VL  - 72
IS  - 4 suplement
SP  - S532
EP  - S533
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4598
ER  - 

@conference{
author = "Ivković, Branka and Božić, Dragana and Kotur-Stevuljević, Jelena and Krajišnik, Danina and Vujić, Zorica",
year = "2022",
abstract = "The global market for antiseptics and disinfectants is growing rapidly, fueled by a
coronavirus pandemic and a possible outbreak of infectious diseases in the future. The
antimicrobial, anti-inflammatory and redox activity of chalcone (1,3-diaryl-2-propen-1-one)
is well documented in the literature. The aim of this study is screening of antimicrobial
activity of chalcones and their saturated derivatives as active pharmaceutical ingredients,
synthesized by Claisen-Schmidt condensation (1). The redox potential of the investigated
compounds was tested in the biological environment using spectrophotometric methods to
determine prooxidant/antioxidant parameters. Within preformulation studies solubility and
compatibility with excipients commonly used in liquid pharmaceutical dosage forms was
performed. All the tested chalcones and their saturated derivatives showed satisfactory
antimicrobial activity, but two saturated chalcones showed the best MIC (0.156 - 1.25 mM)
and were categorized as compounds with strong bacteriostatic activity (2). Solubility of the
chalcons with moderate antimicrobial activity and redox potential was higher than its
minimum bacteriostatic and bactericidal concentration in all the tested solvents (ethanol,
isopropyl alcohol and propylene glycol). Based on the chemical structure and predicted logP
values for saturated chalcones that show stronger antimicrobial activity, better water
solubility is expected. These data could be a starting point for formulations of antiseptics and
disinfectants with lower concentrations of alcohol-based solvents, as the synergism between
saturated chalcone and ethanol and isopropyl alcohol was previously shown. Considering the
lighter color of the saturated chalcone, better aesthetic acceptability of the developed
formulations is expected, since it will not stain the skin and objects during application., Globalno tržište antiseptika i dezinfekcionih sredstava ubrzano raste podstaknuto
pandemijom koronavirusa i moguć im širenjem drugih zaraznih bolesti u buduć nosti.
Antimikrobna, antiinflamatorna i redoks aktivnost halkona (1,3-diaril-2-propen-1-ona) je
dobro dokumentovana u literaturi. Cilj ove studije bio je skrining antimikrobne aktivnosti
halkona i njihovih zasić enih derivata kao aktivnih farmaceutskih sastojaka, sintetisanih
Claisen‐Schmidt kondenzacijom (1). Redoks potencijal ispitivanih jedinjenja je ispitan u
biosredini primenom spektrofotometrijskih metoda za određivanje
prooksidantnih/antioksidativnih parametara. Sprovedena su i preformulaciona ispitivanja
rastvorljivosti i kompatibilnosti sa ekscipijensima uobičajeno korišćenim u tečnim
farmaceutskim oblicima. Svi testirani halkoni i njihovi zasić eni derivati su pokazali
zadovoljavajuć u antimikrobnu aktivnost, ali su dva zasić ena halkona pokazala najbolji MIC
(0,156 – 1,25 mM) i kategorisana su kao jedinjenja sa jakom bakteriostatskom aktivnošć u
(2). Rastvorljivost halkona sa umerenom antimikrobnom aktivnošću i redoks potencijalom
bili su viši od njegove minimalne bakteriostatske i baktericidne koncentracije u svim
ispitivanim rastvaračima (etanol, izopropil alkohol i propilenglikol). Na osnovu hemijske
strukture i predviđene logP vrednosti za zasićene halkone koji pokazuju bolju antimikrobnu
aktivnost, očekuje se i bolja rastvorljivost u vodi. Ovi podaci mogli bi da budu polazna osnova
za formulisanje antiseptika i dezinfekcionih sredstava sa nižim koncentracijama rastvarača
na bazi alkohola, uzimajući u obzir da je u prethodnom ispitivanju pokazan sinergizam
između zasićenog halkona i etanola, kao i izopropil alkohola. S obzirom na svetliju boju
zasićenog halkona, očekuje se i bolja estetska prihvatljivost razvijenih formulacija, jer neć e
bojiti kožu i predmete tokom nanošenja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chalcone derivatives as potential antiseptics and disinfectants, Derivati halkona kao potentijalni antiseptici i dezinficijensi",
volume = "72",
number = "4 suplement",
pages = "S532-S533",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4598"
}

Ivković, B., Božić, D., Kotur-Stevuljević, J., Krajišnik, D.,& Vujić, Z.. (2022). Chalcone derivatives as potential antiseptics and disinfectants. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S532-S533.
https://hdl.handle.net/21.15107/rcub_farfar_4598

Ivković B, Božić D, Kotur-Stevuljević J, Krajišnik D, Vujić Z. Chalcone derivatives as potential antiseptics and disinfectants. in Arhiv za farmaciju. 2022;72(4 suplement):S532-S533.
https://hdl.handle.net/21.15107/rcub_farfar_4598 .

Ivković, Branka, Božić, Dragana, Kotur-Stevuljević, Jelena, Krajišnik, Danina, Vujić, Zorica, "Chalcone derivatives as potential antiseptics and disinfectants" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S532-S533,
https://hdl.handle.net/21.15107/rcub_farfar_4598 .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Savić, Sanela
AU  - Mitrović, Jelena
AU  - Nikolić, Ines
AU  - Marković, Bojan
AU  - Ranđelović, Danijela
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Cekić, Nebojša
AU  - Stevanović, Vladimir
AU  - Batinić, Bojan
AU  - Aranđelović, Jovana
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5541
AB  - The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats
VL  - 22
IS  - 15
DO  - 10.3390/ijms22157991
ER  - 

@article{
author = "Đoković, Jelena and Savić, Sanela and Mitrović, Jelena and Nikolić, Ines and Marković, Bojan and Ranđelović, Danijela and Antić-Stanković, Jelena and Božić, Dragana and Cekić, Nebojša and Stevanović, Vladimir and Batinić, Bojan and Aranđelović, Jovana and Savić, Miroslav and Savić, Snežana",
year = "2021",
abstract = "The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats",
volume = "22",
number = "15",
doi = "10.3390/ijms22157991"
}

Đoković, J., Savić, S., Mitrović, J., Nikolić, I., Marković, B., Ranđelović, D., Antić-Stanković, J., Božić, D., Cekić, N., Stevanović, V., Batinić, B., Aranđelović, J., Savić, M.,& Savić, S.. (2021). Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences
MDPI., 22(15).
https://doi.org/10.3390/ijms22157991

Đoković J, Savić S, Mitrović J, Nikolić I, Marković B, Ranđelović D, Antić-Stanković J, Božić D, Cekić N, Stevanović V, Batinić B, Aranđelović J, Savić M, Savić S. Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences. 2021;22(15).
doi:10.3390/ijms22157991 .

Đoković, Jelena, Savić, Sanela, Mitrović, Jelena, Nikolić, Ines, Marković, Bojan, Ranđelović, Danijela, Antić-Stanković, Jelena, Božić, Dragana, Cekić, Nebojša, Stevanović, Vladimir, Batinić, Bojan, Aranđelović, Jovana, Savić, Miroslav, Savić, Snežana, "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats" in International Journal of Molecular Sciences, 22, no. 15 (2021),
https://doi.org/10.3390/ijms22157991 . .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5097
AB  - The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.
PB  - University of Belgrade
C3  - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
T1  - Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5097
ER  - 

@conference{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2021",
abstract = "The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.",
publisher = "University of Belgrade",
journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts",
title = "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5097"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2021). Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
University of Belgrade., 85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):85-85,
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

TY  - JOUR
AU  - Gledović, Ana
AU  - Janošević-Ležaić, Aleksandra
AU  - Nikolić, Ines
AU  - Tasić-Kostov, Marija
AU  - Antić-Stanković, Jelena
AU  - Krstonošić, Veljko
AU  - Ranđelović, Danijela
AU  - Božić, Dragana
AU  - Ilić, Dušan
AU  - Tamburić, Slobodanka
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3777
AB  - This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.
PB  - MDPI AG
T2  - Nanomaterials
T1  - Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance
VL  - 11
IS  - 1
SP  - 1
EP  - 21
DO  - 10.3390/nano11010217
ER  - 

@article{
author = "Gledović, Ana and Janošević-Ležaić, Aleksandra and Nikolić, Ines and Tasić-Kostov, Marija and Antić-Stanković, Jelena and Krstonošić, Veljko and Ranđelović, Danijela and Božić, Dragana and Ilić, Dušan and Tamburić, Slobodanka and Savić, Snežana",
year = "2021",
abstract = "This study focuses on the development of biocompatible oil-in-water (O/W) nanoemulsions based on polyglycerol esters, as promising carriers for natural actives: red raspberry seed oil—RO and hydro-glycolic fruit extracts from red raspberry—RE and French oak—FE. Nanoemulsions were obtained via phase inversion composition (PIC) method at room temperature by dilution of microemulsion phase, confirmed by visual appearance, percentage of transmittance, microscopic, rheological and Differential Scanning Calorimetry (DSC) investigations. The results have shown that the basic RO-loaded formulation could be further enriched with hydro-glycolic fruit extracts from red raspberry or French oak, while keeping a semi-transparent appearance due to the fine droplet size (Z-ave: 50 to 70 nm, PDI value ≤ 0.1). The highest antioxidant activity (~92% inhibition of the DPPH radical) was achieved in the formulation containing both lipophilic (RO) and hydrophilic antioxidants (FE), due to their synergistic effect. The nanoemulsion carrier significantly increased the selective cytotoxic effect of RO towards malignant melanoma (Fem-X) cells, compared to normal human keratinocytes (HaCaT). In vivo study on human volunteers showed satisfactory safety profiles and significant improvement in skin hydration during 2 h after application for all nanoemulsions. Therefore, polyglycerol ester-based nanoemulsions can be promoted as effective carriers for red raspberry seed oil and/or hydro-glycolic fruit extracts in topical formulations intended for skin protection and hydration.",
publisher = "MDPI AG",
journal = "Nanomaterials",
title = "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance",
volume = "11",
number = "1",
pages = "1-21",
doi = "10.3390/nano11010217"
}

Gledović, A., Janošević-Ležaić, A., Nikolić, I., Tasić-Kostov, M., Antić-Stanković, J., Krstonošić, V., Ranđelović, D., Božić, D., Ilić, D., Tamburić, S.,& Savić, S.. (2021). Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials
MDPI AG., 11(1), 1-21.
https://doi.org/10.3390/nano11010217

Gledović A, Janošević-Ležaić A, Nikolić I, Tasić-Kostov M, Antić-Stanković J, Krstonošić V, Ranđelović D, Božić D, Ilić D, Tamburić S, Savić S. Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance. in Nanomaterials. 2021;11(1):1-21.
doi:10.3390/nano11010217 .

Gledović, Ana, Janošević-Ležaić, Aleksandra, Nikolić, Ines, Tasić-Kostov, Marija, Antić-Stanković, Jelena, Krstonošić, Veljko, Ranđelović, Danijela, Božić, Dragana, Ilić, Dušan, Tamburić, Slobodanka, Savić, Snežana, "Polyglycerol ester-based low energy nanoemulsions with red raspberry seed oil and fruit extracts: Formulation development toward effective in vitro/in vivo bioperformance" in Nanomaterials, 11, no. 1 (2021):1-21,
https://doi.org/10.3390/nano11010217 . .

TY  - JOUR
AU  - Lukić, Milica
AU  - Filipović, Mila
AU  - Pajić, Nevena
AU  - Lunter, Dominique
AU  - Božić, Dragana
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3912
AB  - Objective: The acidic skin pH is one of the regulating factors of skin barrier homeostasis. Topical products as extrinsic factors which influence skin pH could be used for acidification of the skin and consequent beneficial effect. To formulate stabile and safe topical emulsion product with low pH is on-going challenge and areas interesting to explore are related to the effect of acidic products on the skin pH together with development of protocols for these studies. Aim of our work was to investigate formulations of acidic topical products with glycolic acid (GA) stabilized with long chain alkyl polyglucoside emulsifier, in regard to the specific colloidal structure of the vehicle, together with effect of products with different concentration of acidic active on skin pH. Methods: Investigated formulations were basic vehicle and two creams with glycolic acid (concentration 2 and 10 wt%). Microstructure was investigated by polarization microscopy, Raman spectral imaging, thermal analysis and rheological measurements. Effects on the skin were assessed by measurement of biophysical skin parameters in vivo studies (5-hour, 24-hour and 7-days). In vitro screening of antimicrobial activity was performed against bacteria Staphylococcus epidermidis. Results: Polarization micrographs and Raman images have shown that GA does not disturb the specific colloidal structure. Together with rheological and thermal analysis obtained results have shown that GA in higher concentrations contributes to vehicles’ lamellar structure. In 5-hour study the mean values of skin pH ranged from 3.98-4.25 and 3.89-4.10 after application of products with smaller and higher GA concentration. GA samples lowered skin surface pH to 5 and less in 24-hour and 7-day study, with stronger effect of sample with more GA. Sample with 10% of GA had significant inhibitory effect on growth of S. epidermidis in 1:1 concentration. Conclusions: Investigated APG emulsifier could be used as a stabilizer for acidic topical products with GA which are characterized by satisfactory safety profile. Topical products induce acidification of the skin after short- and long-term application without barrier impairment or sign of irritation. Acidification of the skin depends on presence of ingredients which are proton donors and their concentrations.
PB  - Blackwell Publishing Ltd
T2  - International Journal of Cosmetic Science
T1  - Formulation of topical acidic products and acidification of the skin – Contribution of glycolic acid
DO  - 10.1111/ics.12707
ER  - 

@article{
author = "Lukić, Milica and Filipović, Mila and Pajić, Nevena and Lunter, Dominique and Božić, Dragana and Savić, Snežana",
year = "2021",
abstract = "Objective: The acidic skin pH is one of the regulating factors of skin barrier homeostasis. Topical products as extrinsic factors which influence skin pH could be used for acidification of the skin and consequent beneficial effect. To formulate stabile and safe topical emulsion product with low pH is on-going challenge and areas interesting to explore are related to the effect of acidic products on the skin pH together with development of protocols for these studies. Aim of our work was to investigate formulations of acidic topical products with glycolic acid (GA) stabilized with long chain alkyl polyglucoside emulsifier, in regard to the specific colloidal structure of the vehicle, together with effect of products with different concentration of acidic active on skin pH. Methods: Investigated formulations were basic vehicle and two creams with glycolic acid (concentration 2 and 10 wt%). Microstructure was investigated by polarization microscopy, Raman spectral imaging, thermal analysis and rheological measurements. Effects on the skin were assessed by measurement of biophysical skin parameters in vivo studies (5-hour, 24-hour and 7-days). In vitro screening of antimicrobial activity was performed against bacteria Staphylococcus epidermidis. Results: Polarization micrographs and Raman images have shown that GA does not disturb the specific colloidal structure. Together with rheological and thermal analysis obtained results have shown that GA in higher concentrations contributes to vehicles’ lamellar structure. In 5-hour study the mean values of skin pH ranged from 3.98-4.25 and 3.89-4.10 after application of products with smaller and higher GA concentration. GA samples lowered skin surface pH to 5 and less in 24-hour and 7-day study, with stronger effect of sample with more GA. Sample with 10% of GA had significant inhibitory effect on growth of S. epidermidis in 1:1 concentration. Conclusions: Investigated APG emulsifier could be used as a stabilizer for acidic topical products with GA which are characterized by satisfactory safety profile. Topical products induce acidification of the skin after short- and long-term application without barrier impairment or sign of irritation. Acidification of the skin depends on presence of ingredients which are proton donors and their concentrations.",
publisher = "Blackwell Publishing Ltd",
journal = "International Journal of Cosmetic Science",
title = "Formulation of topical acidic products and acidification of the skin – Contribution of glycolic acid",
doi = "10.1111/ics.12707"
}

Lukić, M., Filipović, M., Pajić, N., Lunter, D., Božić, D.,& Savić, S.. (2021). Formulation of topical acidic products and acidification of the skin – Contribution of glycolic acid. in International Journal of Cosmetic Science
Blackwell Publishing Ltd..
https://doi.org/10.1111/ics.12707

Lukić M, Filipović M, Pajić N, Lunter D, Božić D, Savić S. Formulation of topical acidic products and acidification of the skin – Contribution of glycolic acid. in International Journal of Cosmetic Science. 2021;.
doi:10.1111/ics.12707 .

Lukić, Milica, Filipović, Mila, Pajić, Nevena, Lunter, Dominique, Božić, Dragana, Savić, Snežana, "Formulation of topical acidic products and acidification of the skin – Contribution of glycolic acid" in International Journal of Cosmetic Science (2021),
https://doi.org/10.1111/ics.12707 . .

TY  - JOUR
AU  - Ilić, Tijana
AU  - Dodevska, Margarita
AU  - Marčetić, Mirjana
AU  - Božić, Dragana
AU  - Kodranov, Igor
AU  - Vidović, Bojana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3925
AB  - Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a “superfood”
with plenty of health benefits, there is extensive research interest in their nutritional and phytochemical
composition. In the present study, the nutritional value, minerals, fatty acid composition, and bioactive
compounds of L. barbarum L., red, yellow, and black goji berry (L. ruthenicum Murray.) cultivated
in Serbia were investigated. Antioxidant and antimicrobial properties of their methanol extracts
were assessed. Red goji berry had the highest content of fats, dietary fiber, iron, total carotenoids,
and 2-O- -d-glucopyranosyl-l-ascorbic acid (AA-2 G). The yellow goji berry extract showed the
highest level of flavonoids and the most prominent antimicrobial (especially against Gram-negative
bacteria) properties. The highest total phenolic content and the most potent antioxidant activity were
observed for the extract of black goji berry. Therefore, all goji berries could be a valuable source of
bioactive compounds in the food and pharmaceutical industry.
PB  - MDPI AG
T2  - Foods
T1  - Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia
VL  - 9
IS  - 11
DO  - 10.3390/foods9111614
ER  - 

@article{
author = "Ilić, Tijana and Dodevska, Margarita and Marčetić, Mirjana and Božić, Dragana and Kodranov, Igor and Vidović, Bojana",
year = "2020",
abstract = "Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a “superfood”
with plenty of health benefits, there is extensive research interest in their nutritional and phytochemical
composition. In the present study, the nutritional value, minerals, fatty acid composition, and bioactive
compounds of L. barbarum L., red, yellow, and black goji berry (L. ruthenicum Murray.) cultivated
in Serbia were investigated. Antioxidant and antimicrobial properties of their methanol extracts
were assessed. Red goji berry had the highest content of fats, dietary fiber, iron, total carotenoids,
and 2-O- -d-glucopyranosyl-l-ascorbic acid (AA-2 G). The yellow goji berry extract showed the
highest level of flavonoids and the most prominent antimicrobial (especially against Gram-negative
bacteria) properties. The highest total phenolic content and the most potent antioxidant activity were
observed for the extract of black goji berry. Therefore, all goji berries could be a valuable source of
bioactive compounds in the food and pharmaceutical industry.",
publisher = "MDPI AG",
journal = "Foods",
title = "Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia",
volume = "9",
number = "11",
doi = "10.3390/foods9111614"
}

Ilić, T., Dodevska, M., Marčetić, M., Božić, D., Kodranov, I.,& Vidović, B.. (2020). Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia. in Foods
MDPI AG., 9(11).
https://doi.org/10.3390/foods9111614

Ilić T, Dodevska M, Marčetić M, Božić D, Kodranov I, Vidović B. Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia. in Foods. 2020;9(11).
doi:10.3390/foods9111614 .

Ilić, Tijana, Dodevska, Margarita, Marčetić, Mirjana, Božić, Dragana, Kodranov, Igor, Vidović, Bojana, "Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia" in Foods, 9, no. 11 (2020),
https://doi.org/10.3390/foods9111614 . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Ranđelovic, Danijela
AU  - Marković, Bojan
AU  - Lunter, Dominique Jasmin
AU  - Kremenović, Aleksandar
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3711
AB  - As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.
PB  - Walter de Gruyter GmbH
T2  - Reviews on Advanced Materials Science
T1  - Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals
VL  - 59
IS  - 1
SP  - 406
EP  - 424
DO  - 10.1515/rams-2020-0043
DO  - 2-s2.0-85092928784
ER  - 

@article{
author = "Nikolić, Ines and Antić-Stanković, Jelena and Božić, Dragana and Ranđelovic, Danijela and Marković, Bojan and Lunter, Dominique Jasmin and Kremenović, Aleksandar and Savić, Miroslav and Savić, Snežana",
year = "2020",
abstract = "As the number of poorly soluble drugs is increasing, nanocrystals have become very interesting due to wide range of application possibilities. Curcuminwas used as a model active ingredient in this work. Even though it has many proven positive effects, due to its physicochemical issues, its possibilities have not been fully exploited. The goal of this work was to select optimal conditions for a top-down method for curcumin nanosuspension production, and to perform their comprehensive characterization applying complementary methodologies: dynamic light scattering, polarization and atomic force microscopy, thermal analysis, X-ray powder diffraction, antioxidant activity evaluation, release kinetics assessment, and screening of potential biological effects applying cell viability assays on normal human lung fibroblasts, human melanoma and human adenomacarcinoma cells. After 30 min of milling, nanosuspensions stabilized by polysorbate 80 and by its combinations with sucrose palmitate showed good stability, while curcumin crystal structure was unaltered. Obtained nanocrystals were well defined, with average diameter 120-170 nm and PDI of about 0.25, zeta potential was below -30 mV and pH~5 for all formulations. Nanodispersions exhibited high antioxidant potential and improved dissolution rate compared to the corresponding coarse dispersions. Although curcumin nanodispersions exhibited significant antiproliferative effect to each cancer cell line, the highest effect was towards adenocarcinoma cells.",
publisher = "Walter de Gruyter GmbH",
journal = "Reviews on Advanced Materials Science",
title = "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals",
volume = "59",
number = "1",
pages = "406-424",
doi = "10.1515/rams-2020-0043, 2-s2.0-85092928784"
}

Nikolić, I., Antić-Stanković, J., Božić, D., Ranđelovic, D., Marković, B., Lunter, D. J., Kremenović, A., Savić, M.,& Savić, S.. (2020). Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science
Walter de Gruyter GmbH., 59(1), 406-424.
https://doi.org/10.1515/rams-2020-0043

Nikolić I, Antić-Stanković J, Božić D, Ranđelovic D, Marković B, Lunter DJ, Kremenović A, Savić M, Savić S. Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals. in Reviews on Advanced Materials Science. 2020;59(1):406-424.
doi:10.1515/rams-2020-0043 .

Nikolić, Ines, Antić-Stanković, Jelena, Božić, Dragana, Ranđelovic, Danijela, Marković, Bojan, Lunter, Dominique Jasmin, Kremenović, Aleksandar, Savić, Miroslav, Savić, Snežana, "Curcumin Nanonization Using An Alternative Small-Scale Production Unit: Selection of Proper Stabilizer Applying Basic Physicochemical Consideration and Biological Activity Assessment of Nanocrystals" in Reviews on Advanced Materials Science, 59, no. 1 (2020):406-424,
https://doi.org/10.1515/rams-2020-0043 . .

TY  - JOUR
AU  - Milojković, Marko
AU  - Nenadović, Željka
AU  - Stanković, Slaviša
AU  - Božić, Dragana
AU  - Stanković-Nedeljković, Nataša
AU  - Ćirković, Ivana
AU  - Petrović, Marija
AU  - Dimkić, Ivica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3716
AB  - Drug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of isolates. A total of 77.66 % isolates were mostly weak and moderate biofilm producers. Isolates were susceptible to colistin (100 %), aztreonam (97.87 %), imipenem (91.49 %), doripenem (90.43 %), and meropenem (84.04 %). MICs values confirmed susceptibility to ceftazidime and cefepime and singled out meripenem as the most effective inhibitor. Most isolates were resistant to aminoglycosides and fluoroquinolones. Only two isolates produced ESBL, eight were carbapenemase producers, and five isolates produced MBLs. Twenty-nine isolates were multidrug-resistant; 82.8 % of which produced both pigments, 58.3 % were non-typeable, while the P6 and P11 serotypes were equally distributed (16.7 %). Thirteen MDR isolates were strong enzyme producers. RAPD PCR analysis using primer 272 proved the best at discriminatory fingerprinting for Pseudomonas isolates, as it allocated 12 clusters. A correlation between DNA patterns and antibiotic resistance, production of pigments, serotypes distribution, and biofilm formation was not observed, and only confirmed higher genetic heterogeneity among P. aeruginosa isolates, which suggests that other molecular methods are needed to reveal potential relations between genotypic patterns and phenotypic characteristics.
AB  - Antibiotska rezistencija Pseudomonas aeruginosa vodeći je problem u bolničkim infekcijama. Cilj ovoga istraživanja bio je utvrditi najbolju diskriminatorno molekularno-genetičku metodu među 94 ambulantna i bolnička Pseudomonas spp. izolata kako bi se uvidjelo njihovo grupiranje u smislu različitih fenotipskih obilježja (stvaranje biofilma, učestalost serotipova, pigmentacija, proizvodnja različitih klasa beta-laktamaza i osjetljivost na različite skupine antibiotika) u skladu s genotipom. Najčešći serotipovi bili su P1, P6 i P11, a proizvodnja i pioverdina i piocijanina primijećena je kod 70 % izolata. Ukupno 77,66 % izolata uglavnom je iskazalo slabu i umjerenu proizvodnju biofilma. Izolati su bili osjetljivi na kolistin (100 %), aztreonam (97,87 %), imipenem (91,49 %), doripenem (90,43 %) i meropenem (84,04 %). Vrijednosti MIC-ova potvrdile su podložnost izolata ceftazidimu i cefepimu, a izdvojile su meropenem kao najučinkovitiji inhibitor. Većina izolata bila je otporna na aminoglikozid i fluorokinolon. Samo dva izolata proizvela su ESBL, osam izolata sintetiziralo je karbapenemaze, a pet izolata imalo je sposobnost proizvodnje MBL-a. Dvadeset devet izolata bilo je višestruko rezistentno na antibiotike, od kojih je 82,8 % proizvodilo oba pigmenta, 58,3 % bili su netipabilni, a serotipovi P6 i P11 bili su podjednako zastupljeni među njima (16,7 %). Trinaest MDR izolata bili su snažni proizvođači enzima. RAPD PCR analiza korištenjem 272 početnica pokazala se kao najbolja diskriminatorna metoda otiskom prsta (fingerprinting) za Pseudomonas izolate, izdvajajući čak 12 različitih klastera. U ovom istraživanju nije zabilježena povezanost između DNA obrazaca i otpornosti na antibiotike, proizvodnje pigmenata, distribucije serotipova i stvaranja biofilma, što potvrđuje puno veću genetičku heterogenost unutar samih izolata P. aeruginosa, pod čim se podrazumijeva uključivanje drugih molekularnih metoda u otkrivanju potencijalnih odnosa između genetičkih obrazaca i fenotipskih obilježja.
PB  - Sciendo
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia
T1  - Fenotipska i genetička obilježja osjetljivih i višestruko otpornih izolata Pseudomonas aeruginosa u južnoj Srbiji
VL  - 71
IS  - 3
SP  - 231
EP  - 250
DO  - 10.2478/aiht-2020-71-3418
ER  - 

@article{
author = "Milojković, Marko and Nenadović, Željka and Stanković, Slaviša and Božić, Dragana and Stanković-Nedeljković, Nataša and Ćirković, Ivana and Petrović, Marija and Dimkić, Ivica",
year = "2020",
abstract = "Drug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of isolates. A total of 77.66 % isolates were mostly weak and moderate biofilm producers. Isolates were susceptible to colistin (100 %), aztreonam (97.87 %), imipenem (91.49 %), doripenem (90.43 %), and meropenem (84.04 %). MICs values confirmed susceptibility to ceftazidime and cefepime and singled out meripenem as the most effective inhibitor. Most isolates were resistant to aminoglycosides and fluoroquinolones. Only two isolates produced ESBL, eight were carbapenemase producers, and five isolates produced MBLs. Twenty-nine isolates were multidrug-resistant; 82.8 % of which produced both pigments, 58.3 % were non-typeable, while the P6 and P11 serotypes were equally distributed (16.7 %). Thirteen MDR isolates were strong enzyme producers. RAPD PCR analysis using primer 272 proved the best at discriminatory fingerprinting for Pseudomonas isolates, as it allocated 12 clusters. A correlation between DNA patterns and antibiotic resistance, production of pigments, serotypes distribution, and biofilm formation was not observed, and only confirmed higher genetic heterogeneity among P. aeruginosa isolates, which suggests that other molecular methods are needed to reveal potential relations between genotypic patterns and phenotypic characteristics., Antibiotska rezistencija Pseudomonas aeruginosa vodeći je problem u bolničkim infekcijama. Cilj ovoga istraživanja bio je utvrditi najbolju diskriminatorno molekularno-genetičku metodu među 94 ambulantna i bolnička Pseudomonas spp. izolata kako bi se uvidjelo njihovo grupiranje u smislu različitih fenotipskih obilježja (stvaranje biofilma, učestalost serotipova, pigmentacija, proizvodnja različitih klasa beta-laktamaza i osjetljivost na različite skupine antibiotika) u skladu s genotipom. Najčešći serotipovi bili su P1, P6 i P11, a proizvodnja i pioverdina i piocijanina primijećena je kod 70 % izolata. Ukupno 77,66 % izolata uglavnom je iskazalo slabu i umjerenu proizvodnju biofilma. Izolati su bili osjetljivi na kolistin (100 %), aztreonam (97,87 %), imipenem (91,49 %), doripenem (90,43 %) i meropenem (84,04 %). Vrijednosti MIC-ova potvrdile su podložnost izolata ceftazidimu i cefepimu, a izdvojile su meropenem kao najučinkovitiji inhibitor. Većina izolata bila je otporna na aminoglikozid i fluorokinolon. Samo dva izolata proizvela su ESBL, osam izolata sintetiziralo je karbapenemaze, a pet izolata imalo je sposobnost proizvodnje MBL-a. Dvadeset devet izolata bilo je višestruko rezistentno na antibiotike, od kojih je 82,8 % proizvodilo oba pigmenta, 58,3 % bili su netipabilni, a serotipovi P6 i P11 bili su podjednako zastupljeni među njima (16,7 %). Trinaest MDR izolata bili su snažni proizvođači enzima. RAPD PCR analiza korištenjem 272 početnica pokazala se kao najbolja diskriminatorna metoda otiskom prsta (fingerprinting) za Pseudomonas izolate, izdvajajući čak 12 različitih klastera. U ovom istraživanju nije zabilježena povezanost između DNA obrazaca i otpornosti na antibiotike, proizvodnje pigmenata, distribucije serotipova i stvaranja biofilma, što potvrđuje puno veću genetičku heterogenost unutar samih izolata P. aeruginosa, pod čim se podrazumijeva uključivanje drugih molekularnih metoda u otkrivanju potencijalnih odnosa između genetičkih obrazaca i fenotipskih obilježja.",
publisher = "Sciendo",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia, Fenotipska i genetička obilježja osjetljivih i višestruko otpornih izolata Pseudomonas aeruginosa u južnoj Srbiji",
volume = "71",
number = "3",
pages = "231-250",
doi = "10.2478/aiht-2020-71-3418"
}

Milojković, M., Nenadović, Ž., Stanković, S., Božić, D., Stanković-Nedeljković, N., Ćirković, I., Petrović, M.,& Dimkić, I.. (2020). Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Sciendo., 71(3), 231-250.
https://doi.org/10.2478/aiht-2020-71-3418

Milojković M, Nenadović Ž, Stanković S, Božić D, Stanković-Nedeljković N, Ćirković I, Petrović M, Dimkić I. Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2020;71(3):231-250.
doi:10.2478/aiht-2020-71-3418 .

Milojković, Marko, Nenadović, Željka, Stanković, Slaviša, Božić, Dragana, Stanković-Nedeljković, Nataša, Ćirković, Ivana, Petrović, Marija, Dimkić, Ivica, "Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 71, no. 3 (2020):231-250,
https://doi.org/10.2478/aiht-2020-71-3418 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Bašić, Jasmina
AU  - Mandinić, Zoran
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Vujić, Zorica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3579
AB  - Introduction/Objective Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance.Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluoro-phenyl)-1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2-Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains.Methods Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500–31.2 μg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without com-pounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results A total of 500 μg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 μg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 μg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 μg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value.Conclusion Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin produc-tion of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs.
AB  - Увод/Циљ Производња биофилма и пиоцијанина je важан фактор вируленције и антибиотске резистенције бактерије Pseudomonas aeruginosa. Циљ рада је био да се испита инхибиторни ефекат синте-тисаних пропафенонских деривата, 3-(2-флуоро-фенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5OF) и 3-(2-трифлуорометилфенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5CF3), на продукцију биофилма и пиоцијанина код клиничких изолата бактерије Pseudomonas aeruginosa.Методе Ефекат пропафенонских деривата испитан је на девет клиничких изолата и једном стандардном соју бакте-рије P. aeruginosa. Утицај на продукцију биофилма испитан је in vitro, инкубацијом бактерија (0,5 по Макфарланду) са 5OF и 5CF3 (500–31,2 μg/ml), и мерењем оптичке густине на 570 nm. Бактерије у медијуму без испитиваних једињења су биле позитивна контрола, а сам медијум негативна контро-ла. Производени пиоцијанин, који је одређиван мерењем оптичке густине на 520 nm, на коинкубације бактерија са 5CF3 или 5OF (250 и 500 μg/ml), третиран је хлороформом и мешањем хлороформског слојa са HCl.Резултати При концентрацији од 500 μg/ml 5OF је довео до потпуне инхибиције продукције биофилма код свих испи-тиваних сојева (10/10). Инхибиција биофилма са 500 μg/ml5CF3 била је потпуна код 4/10 сојева. При концентрацији 5OF и 5CF3 од 250 μg/ml продукција биофилма код већине испитаних изолата била је слаба, док је при концентрацији 125 μg/ml 5OF односно 5CF3 продукција била умерена. Ниже концентрације 5OF и 5CF3 нису имале инхибиторни ефекат на формирање биофилма. У присуству 500 μg/ml 5OF у 6/10 испитиваних сојева продукција пиоцијанина пала је на мање од 40% у односу на контролну вредност. Иста концентрација (500 μg/ml) 5CF3 снизила је продукцију пиоцијанина на мање од 50% од контроле у 7/9 сојева. При концентрацији 250 μg/ml 5OF или 5CF3 већина сојева продуковала је пиоцијанин изнад 50% у односу на позитивну контролу.Закључак Синтетисани пропафенонски деривати, 5OF и 5CF3, инхибирају продукцију биофилма и пиоцијанина код клиничких сојева бактерије Pseudomonas aeruginosa. До-бијени резултати указују на то да пропафенонски деривати представљају могућа полазна једињења за синтезу нових антипсеудомонасних агенаса.
PB  - Beograd : Srpsko lekarsko društvo
T2  - Srpski Arhiv za Celokupno Lekarstvo
T1  - Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production
VL  - 148
IS  - 3-4
SP  - 196
EP  - 202
DO  - 10.2298/SARH180727102P
ER  - 

@article{
author = "Petrović, Snježana and Bašić, Jasmina and Mandinić, Zoran and Božić, Dragana and Milenković, Marina and Vujić, Zorica",
year = "2020",
abstract = "Introduction/Objective Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance.Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluoro-phenyl)-1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2-Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains.Methods Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500–31.2 μg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without com-pounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results A total of 500 μg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 μg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 μg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 μg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value.Conclusion Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin produc-tion of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs., Увод/Циљ Производња биофилма и пиоцијанина je важан фактор вируленције и антибиотске резистенције бактерије Pseudomonas aeruginosa. Циљ рада је био да се испита инхибиторни ефекат синте-тисаних пропафенонских деривата, 3-(2-флуоро-фенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5OF) и 3-(2-трифлуорометилфенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5CF3), на продукцију биофилма и пиоцијанина код клиничких изолата бактерије Pseudomonas aeruginosa.Методе Ефекат пропафенонских деривата испитан је на девет клиничких изолата и једном стандардном соју бакте-рије P. aeruginosa. Утицај на продукцију биофилма испитан је in vitro, инкубацијом бактерија (0,5 по Макфарланду) са 5OF и 5CF3 (500–31,2 μg/ml), и мерењем оптичке густине на 570 nm. Бактерије у медијуму без испитиваних једињења су биле позитивна контрола, а сам медијум негативна контро-ла. Производени пиоцијанин, који је одређиван мерењем оптичке густине на 520 nm, на коинкубације бактерија са 5CF3 или 5OF (250 и 500 μg/ml), третиран је хлороформом и мешањем хлороформског слојa са HCl.Резултати При концентрацији од 500 μg/ml 5OF је довео до потпуне инхибиције продукције биофилма код свих испи-тиваних сојева (10/10). Инхибиција биофилма са 500 μg/ml5CF3 била је потпуна код 4/10 сојева. При концентрацији 5OF и 5CF3 од 250 μg/ml продукција биофилма код већине испитаних изолата била је слаба, док је при концентрацији 125 μg/ml 5OF односно 5CF3 продукција била умерена. Ниже концентрације 5OF и 5CF3 нису имале инхибиторни ефекат на формирање биофилма. У присуству 500 μg/ml 5OF у 6/10 испитиваних сојева продукција пиоцијанина пала је на мање од 40% у односу на контролну вредност. Иста концентрација (500 μg/ml) 5CF3 снизила је продукцију пиоцијанина на мање од 50% од контроле у 7/9 сојева. При концентрацији 250 μg/ml 5OF или 5CF3 већина сојева продуковала је пиоцијанин изнад 50% у односу на позитивну контролу.Закључак Синтетисани пропафенонски деривати, 5OF и 5CF3, инхибирају продукцију биофилма и пиоцијанина код клиничких сојева бактерије Pseudomonas aeruginosa. До-бијени резултати указују на то да пропафенонски деривати представљају могућа полазна једињења за синтезу нових антипсеудомонасних агенаса.",
publisher = "Beograd : Srpsko lekarsko društvo",
journal = "Srpski Arhiv za Celokupno Lekarstvo",
title = "Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production",
volume = "148",
number = "3-4",
pages = "196-202",
doi = "10.2298/SARH180727102P"
}

Petrović, S., Bašić, J., Mandinić, Z., Božić, D., Milenković, M.,& Vujić, Z.. (2020). Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production. in Srpski Arhiv za Celokupno Lekarstvo
Beograd : Srpsko lekarsko društvo., 148(3-4), 196-202.
https://doi.org/10.2298/SARH180727102P

Petrović S, Bašić J, Mandinić Z, Božić D, Milenković M, Vujić Z. Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production. in Srpski Arhiv za Celokupno Lekarstvo. 2020;148(3-4):196-202.
doi:10.2298/SARH180727102P .

Petrović, Snježana, Bašić, Jasmina, Mandinić, Zoran, Božić, Dragana, Milenković, Marina, Vujić, Zorica, "Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production" in Srpski Arhiv za Celokupno Lekarstvo, 148, no. 3-4 (2020):196-202,
https://doi.org/10.2298/SARH180727102P . .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Obradović, Marijana
AU  - Tomić, Emilija
AU  - Teovanović, Anđelka
AU  - Lakušić, Branislava
AU  - Božić, Dragana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5303
AB  - Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria
SP  - 151
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5303
ER  - 

@conference{
author = "Marčetić, Mirjana and Obradović, Marijana and Tomić, Emilija and Teovanović, Anđelka and Lakušić, Branislava and Božić, Dragana",
year = "2019",
abstract = "Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5303"
}

Marčetić, M., Obradović, M., Tomić, E., Teovanović, A., Lakušić, B.,& Božić, D.. (2019). Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303

Marčetić M, Obradović M, Tomić E, Teovanović A, Lakušić B, Božić D. Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

Marčetić, Mirjana, Obradović, Marijana, Tomić, Emilija, Teovanović, Anđelka, Lakušić, Branislava, Božić, Dragana, "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):151-151,
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Mirjana
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3664
AB  - Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans.
AB  - The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans
T1  - Antifungalna aktivnost etarskog ulja Seseli rigidum Waldst. & Kit. (Apiaceae) na rast izolata Candida albicans
VL  - 69
IS  - 2
SP  - 67
EP  - 79
DO  - 10.5937/arhfarm1902067x
ER  - 

@article{
author = "Marčetić, Mirjana and Božić, Dragana and Milenković, Marina and Kovačević, Mirjana and Kovačević, Nada",
year = "2019",
abstract = "Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans., The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans, Antifungalna aktivnost etarskog ulja Seseli rigidum Waldst. & Kit. (Apiaceae) na rast izolata Candida albicans",
volume = "69",
number = "2",
pages = "67-79",
doi = "10.5937/arhfarm1902067x"
}

Marčetić, M., Božić, D., Milenković, M., Kovačević, M.,& Kovačević, N.. (2019). Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 69(2), 67-79.
https://doi.org/10.5937/arhfarm1902067x

Marčetić M, Božić D, Milenković M, Kovačević M, Kovačević N. Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans. in Arhiv za farmaciju. 2019;69(2):67-79.
doi:10.5937/arhfarm1902067x .

Marčetić, Mirjana, Božić, Dragana, Milenković, Marina, Kovačević, Mirjana, Kovačević, Nada, "Antifungal activity of the esssential oil of Seseli rigidum Waldst. & Kit. (Apiaceae) on the growth of isolates of Candida albicans" in Arhiv za farmaciju, 69, no. 2 (2019):67-79,
https://doi.org/10.5937/arhfarm1902067x . .