TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanovich, Ljudmila
AU  - Đoković, Aleksandra
AU  - Todić, Brankica
AU  - Dopsaj, Violeta
AU  - Saponjski, Jovica
AU  - Saponjski, Dušan
AU  - Marković, Olivera
AU  - Belizna, Cristina
AU  - Zdravković, Marija
AU  - Marisavljević, Dragomir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4310
AB  - Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients
VL  - 23
IS  - 20
DO  - 10.3390/ijms232012309
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanovich, Ljudmila and Đoković, Aleksandra and Todić, Brankica and Dopsaj, Violeta and Saponjski, Jovica and Saponjski, Dušan and Marković, Olivera and Belizna, Cristina and Zdravković, Marija and Marisavljević, Dragomir",
year = "2022",
abstract = "Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients",
volume = "23",
number = "20",
doi = "10.3390/ijms232012309"
}

Stanisavljević, N., Stojanovich, L., Đoković, A., Todić, B., Dopsaj, V., Saponjski, J., Saponjski, D., Marković, O., Belizna, C., Zdravković, M.,& Marisavljević, D.. (2022). Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences
MDPI., 23(20).
https://doi.org/10.3390/ijms232012309

Stanisavljević N, Stojanovich L, Đoković A, Todić B, Dopsaj V, Saponjski J, Saponjski D, Marković O, Belizna C, Zdravković M, Marisavljević D. Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences. 2022;23(20).
doi:10.3390/ijms232012309 .

Stanisavljević, Nataša, Stojanovich, Ljudmila, Đoković, Aleksandra, Todić, Brankica, Dopsaj, Violeta, Saponjski, Jovica, Saponjski, Dušan, Marković, Olivera, Belizna, Cristina, Zdravković, Marija, Marisavljević, Dragomir, "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients" in International Journal of Molecular Sciences, 23, no. 20 (2022),
https://doi.org/10.3390/ijms232012309 . .

TY  - CONF
AU  - Stojanović, Ljudmila
AU  - Stanisavljević, Nataša
AU  - Marisavljević, D.
AU  - Dopsaj, Violeta
AU  - Todić, B.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2648
PB  - BMJ PUBLISHING GROUP, LONDON
C3  - Annals of the Rheumatic Diseases
T1  - The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome
VL  - 75
IS  - Supplement 2
SP  - 284
EP  - 284
DO  - 10.1136/annrheumdis-2016-eular.1782
ER  - 

@conference{
author = "Stojanović, Ljudmila and Stanisavljević, Nataša and Marisavljević, D. and Dopsaj, Violeta and Todić, B.",
year = "2016",
publisher = "BMJ PUBLISHING GROUP, LONDON",
journal = "Annals of the Rheumatic Diseases",
title = "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome",
volume = "75",
number = "Supplement 2",
pages = "284-284",
doi = "10.1136/annrheumdis-2016-eular.1782"
}

Stojanović, L., Stanisavljević, N., Marisavljević, D., Dopsaj, V.,& Todić, B.. (2016). The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases
BMJ PUBLISHING GROUP, LONDON., 75(Supplement 2), 284-284.
https://doi.org/10.1136/annrheumdis-2016-eular.1782

Stojanović L, Stanisavljević N, Marisavljević D, Dopsaj V, Todić B. The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases. 2016;75(Supplement 2):284-284.
doi:10.1136/annrheumdis-2016-eular.1782 .

Stojanović, Ljudmila, Stanisavljević, Nataša, Marisavljević, D., Dopsaj, Violeta, Todić, B., "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome" in Annals of the Rheumatic Diseases, 75, no. Supplement 2 (2016):284-284,
https://doi.org/10.1136/annrheumdis-2016-eular.1782 . .

TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanović, Ljudmila
AU  - Marisavljević, D.
AU  - Đoković, A.
AU  - Dopsaj, Violeta
AU  - Kotur-Stevuljević, Jelena
AU  - Martinović, Jelena
AU  - Memon, Lidija
AU  - Radovanović, Slavica
AU  - Todić, B.
AU  - Lisulov, D.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2710
AB  - The aim of this study was to evaluate oxidative stress markers and it relations to endothelial damage as risk factor for thrombosis in patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome (APS) in correlation to traditional risk factors. Flow-mediated (FMD) and nitroglycerine (NMD)-induced dilation of the brachial artery were studied in 140 APS patients (90 PAPS, 50 SAPS) and 40 controls matched by age, sex, and conventional risk factors for atherosclerosis. Markers of oxidative stress, lipid hydroperoxydes (LOOH), advanced oxidation protein products (AOPP), total sulfhydryl groups (tSHG), and paraoxonase 1 activity (PON1) were determined by spectrophotometric method. Oxidative stress dominates in APS patients. LOOH and AOPP correlate to lipid fractions (p  lt  0.05), unlike PON1, tSHG that correlated to antiphospholipid antibody positivity (p  lt  0.05). FMD was lower in APS patients comparing to controls (p  lt  0.001). Cholesterol is independent variable for FMD impairment in control group (p = 0.011); LOOH in PAPS (p = 0.004); LOOH, aCL, and triglycerides in SAPS patients (p = 0.009, p = 0.049, and p = 0.012, respectively). Combined predictive of aCL and LOOH is better for FMD impairment than LOOH alone in both PAPS and SAPS patients (AUC 0.727, p = 0.001, 95 % CI 0.616-0.837 and AUC 0.824, pE,0.001, 95 % CI 0.690-0.957, respectively). Lipid peroxidation is independent predictor for endothelial dysfunction in APS patients. We demonstrated synergistic effect of aCL and LOOH as risk for endothelial impairment in both PAPS and SAPS patients.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients
VL  - 35
IS  - 10
SP  - 2485
EP  - 2493
DO  - 10.1007/s10067-016-3369-8
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanović, Ljudmila and Marisavljević, D. and Đoković, A. and Dopsaj, Violeta and Kotur-Stevuljević, Jelena and Martinović, Jelena and Memon, Lidija and Radovanović, Slavica and Todić, B. and Lisulov, D.",
year = "2016",
abstract = "The aim of this study was to evaluate oxidative stress markers and it relations to endothelial damage as risk factor for thrombosis in patients with primary (PAPS) and secondary (SAPS) antiphospholipid syndrome (APS) in correlation to traditional risk factors. Flow-mediated (FMD) and nitroglycerine (NMD)-induced dilation of the brachial artery were studied in 140 APS patients (90 PAPS, 50 SAPS) and 40 controls matched by age, sex, and conventional risk factors for atherosclerosis. Markers of oxidative stress, lipid hydroperoxydes (LOOH), advanced oxidation protein products (AOPP), total sulfhydryl groups (tSHG), and paraoxonase 1 activity (PON1) were determined by spectrophotometric method. Oxidative stress dominates in APS patients. LOOH and AOPP correlate to lipid fractions (p  lt  0.05), unlike PON1, tSHG that correlated to antiphospholipid antibody positivity (p  lt  0.05). FMD was lower in APS patients comparing to controls (p  lt  0.001). Cholesterol is independent variable for FMD impairment in control group (p = 0.011); LOOH in PAPS (p = 0.004); LOOH, aCL, and triglycerides in SAPS patients (p = 0.009, p = 0.049, and p = 0.012, respectively). Combined predictive of aCL and LOOH is better for FMD impairment than LOOH alone in both PAPS and SAPS patients (AUC 0.727, p = 0.001, 95 % CI 0.616-0.837 and AUC 0.824, pE,0.001, 95 % CI 0.690-0.957, respectively). Lipid peroxidation is independent predictor for endothelial dysfunction in APS patients. We demonstrated synergistic effect of aCL and LOOH as risk for endothelial impairment in both PAPS and SAPS patients.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients",
volume = "35",
number = "10",
pages = "2485-2493",
doi = "10.1007/s10067-016-3369-8"
}

Stanisavljević, N., Stojanović, L., Marisavljević, D., Đoković, A., Dopsaj, V., Kotur-Stevuljević, J., Martinović, J., Memon, L., Radovanović, S., Todić, B.,& Lisulov, D.. (2016). Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients. in Clinical Rheumatology
Springer London Ltd, London., 35(10), 2485-2493.
https://doi.org/10.1007/s10067-016-3369-8

Stanisavljević N, Stojanović L, Marisavljević D, Đoković A, Dopsaj V, Kotur-Stevuljević J, Martinović J, Memon L, Radovanović S, Todić B, Lisulov D. Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients. in Clinical Rheumatology. 2016;35(10):2485-2493.
doi:10.1007/s10067-016-3369-8 .

Stanisavljević, Nataša, Stojanović, Ljudmila, Marisavljević, D., Đoković, A., Dopsaj, Violeta, Kotur-Stevuljević, Jelena, Martinović, Jelena, Memon, Lidija, Radovanović, Slavica, Todić, B., Lisulov, D., "Lipid peroxidation as risk factor for endothelial dysfunction in antiphospholipid syndrome patients" in Clinical Rheumatology, 35, no. 10 (2016):2485-2493,
https://doi.org/10.1007/s10067-016-3369-8 . .