TY  - JOUR
AU  - Pejić, Nataša
AU  - Kuntić, Vesna
AU  - Vujić, Zorica
AU  - Micić, S
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/558
AB  - The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34°C and I = 7.5 × 10-5 M. Beer's law is obeyed in the concentration range 1.0-12.0 μg ml-1 for quercetin. The corresponding detection limit is 0.76 μg ml-1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab®.
PB  - Elsevier Masson SAS
T2  - Farmaco
T1  - Direct spectrophotometric determination of quercetin in the presence of ascorbic acid
VL  - 59
IS  - 1
SP  - 21
EP  - 24
DO  - 10.1016/j.farmac.2003.07.013
ER  - 

@article{
author = "Pejić, Nataša and Kuntić, Vesna and Vujić, Zorica and Micić, S",
year = "2004",
abstract = "The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34°C and I = 7.5 × 10-5 M. Beer's law is obeyed in the concentration range 1.0-12.0 μg ml-1 for quercetin. The corresponding detection limit is 0.76 μg ml-1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab®.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Direct spectrophotometric determination of quercetin in the presence of ascorbic acid",
volume = "59",
number = "1",
pages = "21-24",
doi = "10.1016/j.farmac.2003.07.013"
}

Pejić, N., Kuntić, V., Vujić, Z.,& Micić, S.. (2004). Direct spectrophotometric determination of quercetin in the presence of ascorbic acid. in Farmaco
Elsevier Masson SAS., 59(1), 21-24.
https://doi.org/10.1016/j.farmac.2003.07.013

Pejić N, Kuntić V, Vujić Z, Micić S. Direct spectrophotometric determination of quercetin in the presence of ascorbic acid. in Farmaco. 2004;59(1):21-24.
doi:10.1016/j.farmac.2003.07.013 .

Pejić, Nataša, Kuntić, Vesna, Vujić, Zorica, Micić, S, "Direct spectrophotometric determination of quercetin in the presence of ascorbic acid" in Farmaco, 59, no. 1 (2004):21-24,
https://doi.org/10.1016/j.farmac.2003.07.013 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Micić, S
AU  - Malešev, Dušan
AU  - Vujić, Zorica
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/421
PB  - Govi-Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Determination of dissociation constants of quercetin
VL  - 58
IS  - 6
SP  - 439
EP  - 440
UR  - https://hdl.handle.net/21.15107/rcub_farfar_421
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Micić, S and Malešev, Dušan and Vujić, Zorica",
year = "2003",
publisher = "Govi-Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Determination of dissociation constants of quercetin",
volume = "58",
number = "6",
pages = "439-440",
url = "https://hdl.handle.net/21.15107/rcub_farfar_421"
}

Kuntić, V., Pejić, N., Micić, S., Malešev, D.,& Vujić, Z.. (2003). Determination of dissociation constants of quercetin. in Pharmazie
Govi-Verlag Gmbh, Eschborn., 58(6), 439-440.
https://hdl.handle.net/21.15107/rcub_farfar_421

Kuntić V, Pejić N, Micić S, Malešev D, Vujić Z. Determination of dissociation constants of quercetin. in Pharmazie. 2003;58(6):439-440.
https://hdl.handle.net/21.15107/rcub_farfar_421 .

Kuntić, Vesna, Pejić, Nataša, Micić, S, Malešev, Dušan, Vujić, Zorica, "Determination of dissociation constants of quercetin" in Pharmazie, 58, no. 6 (2003):439-440,
https://hdl.handle.net/21.15107/rcub_farfar_421 .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Ugrešić, Nenad
AU  - Pejčić-Karapetrović, Branka
AU  - Micić, M
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/251
AB  - It has been hypothesized that maturational processes within the hypothalamo-pituitary-gonadal (HPG) axis and thymus are reciprocally regulated via neural pathways. To test this hypothesis, in the thymi of adult rats orchidectomized (ORX) at age of 1 (ORX-1), 7 (ORX-7) and 30 days (ORX-30): (i) noradrenaline (NA), dopamine (DA) and serotonin (5-HT) contents and acetylcholinesterase (AChE) activity were measured and (ii) the distribution of monoamine- and AChE-containing nerves and cells was examined by a sucrose phosphate glyoxylic acid (SPG) method and enzyme histochemistry, respectively. In all groups of ORX rats, the thymus weight was significantly increased over that in sham-ORX control rats. In the ORX-1 rats, the increase in the thymus weight was accompanied by a proportional increase in the content of both catecholamines and 5-HT; consequently the concentration of each of them remained unaltered. In these animals, the density of both SPG-stained thymus nerve fibers and cells also remained unchanged. In the ORX-7 rats, the rise in the thymus weight was followed by a proportional increase in the content of all monoamines, except for NA which was reduced. Therefore, in these rats neither the thymus concentrations of DA nor that of 5-HT differed from controls, while the concentration of NA was significantly decreased. The reduction in both NA content and concentration reflected a diminished density of SPG-positive nerve profiles. In the ORX-30 rats, the increase in thymus weight was neither paralleled by a proportional increase in the DA content nor in 5-HT, while the content of NA was decreased. Thus, in their thymi the concentration of both NA and DA, as well as that of 5-HT, were significantly reduced. In parallel with these changes, a decreased density of thymic SPG-positive nerve fibers and cells was found. In all ORX rats, the pattern of intrathymic distribution of SPG-positive fibers and cells remained unchanged. Orchidectomy affected neither the activity of AChE (expressed per gram of tissue) nor the density of AChE-positive nerves and cells in the thymus. As the changes in the density of adrenergic nerve fibers in the thymus from ORX rats were not followed by similar alterations in the density of AChE-containing nerve fibers, it does not seem likely that NA and AChE are colocalized in the thymus nerve fibers. The results also suggest that there is a critical period during ontogenesis when changes within the HPG axis evoked by orchidectomy can affect the sympathetic nerve input to the rat thymus and therefore, most likely, development and function of the organ. Copyright
PB  - Karger, Basel
T2  - NeuroImmunoModulation
T1  - Castration of sexually immature rats affects sympathetic innervation of the adult thymus
VL  - 7
IS  - 2
SP  - 59
EP  - 67
DO  - 10.1159/000026421
ER  - 

@article{
author = "Leposavić, Gordana and Ugrešić, Nenad and Pejčić-Karapetrović, Branka and Micić, M",
year = "2000",
abstract = "It has been hypothesized that maturational processes within the hypothalamo-pituitary-gonadal (HPG) axis and thymus are reciprocally regulated via neural pathways. To test this hypothesis, in the thymi of adult rats orchidectomized (ORX) at age of 1 (ORX-1), 7 (ORX-7) and 30 days (ORX-30): (i) noradrenaline (NA), dopamine (DA) and serotonin (5-HT) contents and acetylcholinesterase (AChE) activity were measured and (ii) the distribution of monoamine- and AChE-containing nerves and cells was examined by a sucrose phosphate glyoxylic acid (SPG) method and enzyme histochemistry, respectively. In all groups of ORX rats, the thymus weight was significantly increased over that in sham-ORX control rats. In the ORX-1 rats, the increase in the thymus weight was accompanied by a proportional increase in the content of both catecholamines and 5-HT; consequently the concentration of each of them remained unaltered. In these animals, the density of both SPG-stained thymus nerve fibers and cells also remained unchanged. In the ORX-7 rats, the rise in the thymus weight was followed by a proportional increase in the content of all monoamines, except for NA which was reduced. Therefore, in these rats neither the thymus concentrations of DA nor that of 5-HT differed from controls, while the concentration of NA was significantly decreased. The reduction in both NA content and concentration reflected a diminished density of SPG-positive nerve profiles. In the ORX-30 rats, the increase in thymus weight was neither paralleled by a proportional increase in the DA content nor in 5-HT, while the content of NA was decreased. Thus, in their thymi the concentration of both NA and DA, as well as that of 5-HT, were significantly reduced. In parallel with these changes, a decreased density of thymic SPG-positive nerve fibers and cells was found. In all ORX rats, the pattern of intrathymic distribution of SPG-positive fibers and cells remained unchanged. Orchidectomy affected neither the activity of AChE (expressed per gram of tissue) nor the density of AChE-positive nerves and cells in the thymus. As the changes in the density of adrenergic nerve fibers in the thymus from ORX rats were not followed by similar alterations in the density of AChE-containing nerve fibers, it does not seem likely that NA and AChE are colocalized in the thymus nerve fibers. The results also suggest that there is a critical period during ontogenesis when changes within the HPG axis evoked by orchidectomy can affect the sympathetic nerve input to the rat thymus and therefore, most likely, development and function of the organ. Copyright",
publisher = "Karger, Basel",
journal = "NeuroImmunoModulation",
title = "Castration of sexually immature rats affects sympathetic innervation of the adult thymus",
volume = "7",
number = "2",
pages = "59-67",
doi = "10.1159/000026421"
}

Leposavić, G., Ugrešić, N., Pejčić-Karapetrović, B.,& Micić, M.. (2000). Castration of sexually immature rats affects sympathetic innervation of the adult thymus. in NeuroImmunoModulation
Karger, Basel., 7(2), 59-67.
https://doi.org/10.1159/000026421

Leposavić G, Ugrešić N, Pejčić-Karapetrović B, Micić M. Castration of sexually immature rats affects sympathetic innervation of the adult thymus. in NeuroImmunoModulation. 2000;7(2):59-67.
doi:10.1159/000026421 .

Leposavić, Gordana, Ugrešić, Nenad, Pejčić-Karapetrović, Branka, Micić, M, "Castration of sexually immature rats affects sympathetic innervation of the adult thymus" in NeuroImmunoModulation, 7, no. 2 (2000):59-67,
https://doi.org/10.1159/000026421 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Pejčić-Karapetrović, Branka
AU  - Micić, M
AU  - Ugrešić, Nenad
PY  - 1999
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/217
AB  - To assess a putative role of the neural pathways in transfer of information from the gonads to the thymus, adult AO rats were orchidectomized (ORX) or sham ORX (controls); sacrificed 1, 3, 7, or 15 days later and their thymi were analyzed for: (a) the concentrations of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) and distribution of monoamine-containing nerve profiles and (b) the acetylcholinesterase (AChE) activity and distribution of AChE-containing nerve profiles. Three days after the castration, an elevation in the level of both catecholamines, reflecting an increase in the overall intensity of nerve fibers autofluorescence, was found. Seven days post castration neither NA nor DA concentration differed from the appropriate control values, while 15 days after the surgery the concentration of NA was lower than that in the controls, most likely, due to diminished density of noradrenergic nerve profiles. In both the rats sacrificed 7 and 15 days after orchidectomy the concentration of 5-HT was reduced as result of a decrease in the density of 5-HT-containing autofluorescent cells. The activity of AChE was depressed one day after the surgery; then increased, so that 3 days post castration its value was higher than that in the sham ORX. After this increase, AChE activity decreased being, at postoperative day 7 and 15, lower than that in the controls. It seems that this decrease in AChE activity reflected, not only a reduction in the density of AChE-containing nerve fibers, but also a decrease in the density of AChE positive cells. Thus, the results indicate that orchidectomy can evoke changes in the T-cell maturation altering modulatory influences on this process coming via neural route, as well as those coming from the mast cells and AChE positive epithelial cells which constitute important component of the thymus microenvironment.
PB  - Gordon Breach Sci Publ Ltd, Reading
T2  - International Journal of Neuroscience
T1  - Orchidectomy affects the innervation of the rat thymus
VL  - 97
IS  - 1-2
SP  - 115
EP  - 129
DO  - 10.3109/00207459908994306
ER  - 

@article{
author = "Leposavić, Gordana and Pejčić-Karapetrović, Branka and Micić, M and Ugrešić, Nenad",
year = "1999",
abstract = "To assess a putative role of the neural pathways in transfer of information from the gonads to the thymus, adult AO rats were orchidectomized (ORX) or sham ORX (controls); sacrificed 1, 3, 7, or 15 days later and their thymi were analyzed for: (a) the concentrations of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) and distribution of monoamine-containing nerve profiles and (b) the acetylcholinesterase (AChE) activity and distribution of AChE-containing nerve profiles. Three days after the castration, an elevation in the level of both catecholamines, reflecting an increase in the overall intensity of nerve fibers autofluorescence, was found. Seven days post castration neither NA nor DA concentration differed from the appropriate control values, while 15 days after the surgery the concentration of NA was lower than that in the controls, most likely, due to diminished density of noradrenergic nerve profiles. In both the rats sacrificed 7 and 15 days after orchidectomy the concentration of 5-HT was reduced as result of a decrease in the density of 5-HT-containing autofluorescent cells. The activity of AChE was depressed one day after the surgery; then increased, so that 3 days post castration its value was higher than that in the sham ORX. After this increase, AChE activity decreased being, at postoperative day 7 and 15, lower than that in the controls. It seems that this decrease in AChE activity reflected, not only a reduction in the density of AChE-containing nerve fibers, but also a decrease in the density of AChE positive cells. Thus, the results indicate that orchidectomy can evoke changes in the T-cell maturation altering modulatory influences on this process coming via neural route, as well as those coming from the mast cells and AChE positive epithelial cells which constitute important component of the thymus microenvironment.",
publisher = "Gordon Breach Sci Publ Ltd, Reading",
journal = "International Journal of Neuroscience",
title = "Orchidectomy affects the innervation of the rat thymus",
volume = "97",
number = "1-2",
pages = "115-129",
doi = "10.3109/00207459908994306"
}

Leposavić, G., Pejčić-Karapetrović, B., Micić, M.,& Ugrešić, N.. (1999). Orchidectomy affects the innervation of the rat thymus. in International Journal of Neuroscience
Gordon Breach Sci Publ Ltd, Reading., 97(1-2), 115-129.
https://doi.org/10.3109/00207459908994306

Leposavić G, Pejčić-Karapetrović B, Micić M, Ugrešić N. Orchidectomy affects the innervation of the rat thymus. in International Journal of Neuroscience. 1999;97(1-2):115-129.
doi:10.3109/00207459908994306 .

Leposavić, Gordana, Pejčić-Karapetrović, Branka, Micić, M, Ugrešić, Nenad, "Orchidectomy affects the innervation of the rat thymus" in International Journal of Neuroscience, 97, no. 1-2 (1999):115-129,
https://doi.org/10.3109/00207459908994306 . .