TY  - JOUR
AU  - Kovacević, I
AU  - Miljković, Branislava
AU  - Jovanović, D
AU  - Prostran, Milica
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/845
AB  - A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV  lt  9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
T1  - Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma
VL  - 830
IS  - 2
SP  - 372
EP  - 376
DO  - 10.1016/j.jchromb.2005.11.034
ER  - 

@article{
author = "Kovacević, I and Miljković, Branislava and Jovanović, D and Prostran, Milica",
year = "2006",
abstract = "A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV  lt  9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",
title = "Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma",
volume = "830",
number = "2",
pages = "372-376",
doi = "10.1016/j.jchromb.2005.11.034"
}

Kovacević, I., Miljković, B., Jovanović, D.,& Prostran, M.. (2006). Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Elsevier Science BV, Amsterdam., 830(2), 372-376.
https://doi.org/10.1016/j.jchromb.2005.11.034

Kovacević I, Miljković B, Jovanović D, Prostran M. Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2006;830(2):372-376.
doi:10.1016/j.jchromb.2005.11.034 .

Kovacević, I, Miljković, Branislava, Jovanović, D, Prostran, Milica, "Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 830, no. 2 (2006):372-376,
https://doi.org/10.1016/j.jchromb.2005.11.034 . .

TY  - JOUR
AU  - Đorđević, S
AU  - Kovacević, I
AU  - Miljković, Branislava
AU  - Vuksanović, J
AU  - Pokrajac, Milena
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/641
AB  - A selective, sensitive, simple, and rapid method for the simultaneous determination of fluoxetine (FL) and norfluoxetine (nor-FL) was developed and validated, and further applied to analyze plasma samples obtained from FL-treated patients with Parkinson disease (n:=:18). After one step liquid-liquid extraction with ethyl acetate, plasma samples were chromatographed on a C8 column. The mobile phase was acetate buffer and acetonitrile (40:60 v/v). Determination of FL and nor-FL was performed with MS detection in selective ion monitoring (SIM) mode, so the other components did not interfere with this assay. FL, nor-FL and flumazenil as internal standard were eluted in 6:min. Recoveries ranged from 89.7 to 96.6% and from 80.2 to 85.3% for FL and nor-FL, respectively. The limit of quantitation under described conditions was 2,5 μg/l for FL and 10 μg/l for nor-FL. The method was found to be reproducible with coefficient of variation less than 9%. The parameters of the method were found to be acceptable to enable its routine use for clinical studies. The method was employed to analyze the Parkinsonian patients' plasma samples. A great deviation in plasma concentrations of FL and nor-FL found among 18 studied patients indicates high pharmacokinetic variability of the drug. Obtained results also indicate absence of the influence of Parkinson disease on the drug disposition.
PB  - Elsevier Masson SAS
T2  - Farmaco
T1  - Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study
VL  - 60
IS  - 4
SP  - 345
EP  - 349
DO  - 10.1016/j.farmac.2005.01.002
ER  - 

@article{
author = "Đorđević, S and Kovacević, I and Miljković, Branislava and Vuksanović, J and Pokrajac, Milena",
year = "2005",
abstract = "A selective, sensitive, simple, and rapid method for the simultaneous determination of fluoxetine (FL) and norfluoxetine (nor-FL) was developed and validated, and further applied to analyze plasma samples obtained from FL-treated patients with Parkinson disease (n:=:18). After one step liquid-liquid extraction with ethyl acetate, plasma samples were chromatographed on a C8 column. The mobile phase was acetate buffer and acetonitrile (40:60 v/v). Determination of FL and nor-FL was performed with MS detection in selective ion monitoring (SIM) mode, so the other components did not interfere with this assay. FL, nor-FL and flumazenil as internal standard were eluted in 6:min. Recoveries ranged from 89.7 to 96.6% and from 80.2 to 85.3% for FL and nor-FL, respectively. The limit of quantitation under described conditions was 2,5 μg/l for FL and 10 μg/l for nor-FL. The method was found to be reproducible with coefficient of variation less than 9%. The parameters of the method were found to be acceptable to enable its routine use for clinical studies. The method was employed to analyze the Parkinsonian patients' plasma samples. A great deviation in plasma concentrations of FL and nor-FL found among 18 studied patients indicates high pharmacokinetic variability of the drug. Obtained results also indicate absence of the influence of Parkinson disease on the drug disposition.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study",
volume = "60",
number = "4",
pages = "345-349",
doi = "10.1016/j.farmac.2005.01.002"
}

Đorđević, S., Kovacević, I., Miljković, B., Vuksanović, J.,& Pokrajac, M.. (2005). Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study. in Farmaco
Elsevier Masson SAS., 60(4), 345-349.
https://doi.org/10.1016/j.farmac.2005.01.002

Đorđević S, Kovacević I, Miljković B, Vuksanović J, Pokrajac M. Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study. in Farmaco. 2005;60(4):345-349.
doi:10.1016/j.farmac.2005.01.002 .

Đorđević, S, Kovacević, I, Miljković, Branislava, Vuksanović, J, Pokrajac, Milena, "Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: Application to clinical study" in Farmaco, 60, no. 4 (2005):345-349,
https://doi.org/10.1016/j.farmac.2005.01.002 . .

TY  - JOUR
AU  - Parojčić, Jelena
AU  - Đurić, Zorica
AU  - Jovanović, M
AU  - Ibrić, Svetlana
AU  - Kilibarda, Vesna
AU  - Jovanović, D
AU  - Kovacević, I
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/594
AB  - With the increased interest in modified release dosage forms and drug delivery systems, there is an increasing concern for biopharmaceutical characterization of the formulation in the early stages of drug product development. The main objectives of biopharmaceutical characterization are the in vitro and in vivo evaluation of the selected formulations in order to identify the factors influencing drug release; define the in vitro test methodology that would be predictive of drug products in vivo behavior and develop quantitative in vitro - in vivo correlation. The purpose of this study was to assess the potential of novel carbomer polymers, Carbopol 971P and Carbopol 71G, as a sustained release agents in matrix tablets containing high dosage drug substance. Although chemically identical, the two polymers exhibited substantially different drug release properties in vitro. Hypothetical in vivo drug release profiles were calculated by numerical deconvolution from cumulative urinary excretion data observed in vivo. The obtained results indicated that sound and reliable in vivo drug release profiles could be obtained from urinary excretion data and also, emphasized the need for in vitro testing under a range of experimental conditions in order to develop the biorelevant drug release methodology.
PB  - Springer France, Paris
T2  - European Journal of Drug Metabolism and Pharmacokinetics
T1  - Biopharmaceutical characterization of sustained release matrix tablets based on novel carbomer polymers: formulation and in vivo investigation
VL  - 30
IS  - 1-2
SP  - 99
EP  - 104
DO  - 10.1007/BF03226414
ER  - 

@article{
author = "Parojčić, Jelena and Đurić, Zorica and Jovanović, M and Ibrić, Svetlana and Kilibarda, Vesna and Jovanović, D and Kovacević, I",
year = "2005",
abstract = "With the increased interest in modified release dosage forms and drug delivery systems, there is an increasing concern for biopharmaceutical characterization of the formulation in the early stages of drug product development. The main objectives of biopharmaceutical characterization are the in vitro and in vivo evaluation of the selected formulations in order to identify the factors influencing drug release; define the in vitro test methodology that would be predictive of drug products in vivo behavior and develop quantitative in vitro - in vivo correlation. The purpose of this study was to assess the potential of novel carbomer polymers, Carbopol 971P and Carbopol 71G, as a sustained release agents in matrix tablets containing high dosage drug substance. Although chemically identical, the two polymers exhibited substantially different drug release properties in vitro. Hypothetical in vivo drug release profiles were calculated by numerical deconvolution from cumulative urinary excretion data observed in vivo. The obtained results indicated that sound and reliable in vivo drug release profiles could be obtained from urinary excretion data and also, emphasized the need for in vitro testing under a range of experimental conditions in order to develop the biorelevant drug release methodology.",
publisher = "Springer France, Paris",
journal = "European Journal of Drug Metabolism and Pharmacokinetics",
title = "Biopharmaceutical characterization of sustained release matrix tablets based on novel carbomer polymers: formulation and in vivo investigation",
volume = "30",
number = "1-2",
pages = "99-104",
doi = "10.1007/BF03226414"
}

Parojčić, J., Đurić, Z., Jovanović, M., Ibrić, S., Kilibarda, V., Jovanović, D.,& Kovacević, I.. (2005). Biopharmaceutical characterization of sustained release matrix tablets based on novel carbomer polymers: formulation and in vivo investigation. in European Journal of Drug Metabolism and Pharmacokinetics
Springer France, Paris., 30(1-2), 99-104.
https://doi.org/10.1007/BF03226414

Parojčić J, Đurić Z, Jovanović M, Ibrić S, Kilibarda V, Jovanović D, Kovacević I. Biopharmaceutical characterization of sustained release matrix tablets based on novel carbomer polymers: formulation and in vivo investigation. in European Journal of Drug Metabolism and Pharmacokinetics. 2005;30(1-2):99-104.
doi:10.1007/BF03226414 .

Parojčić, Jelena, Đurić, Zorica, Jovanović, M, Ibrić, Svetlana, Kilibarda, Vesna, Jovanović, D, Kovacević, I, "Biopharmaceutical characterization of sustained release matrix tablets based on novel carbomer polymers: formulation and in vivo investigation" in European Journal of Drug Metabolism and Pharmacokinetics, 30, no. 1-2 (2005):99-104,
https://doi.org/10.1007/BF03226414 . .

TY  - CONF
AU  - Pokrajac, Milena
AU  - Kovacević, I
AU  - Miljković, Branislava
AU  - Mijajlović, M
AU  - Džoljić, Eleonora
AU  - Kostić, V
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/520
PB  - Taylor & Francis Inc, Philadelphia
C3  - Drug Metabolism Reviews
T1  - The monitoring of fluoxetine/norfluoxetine plasma levels for rational use of fluoxetine in depressed Parkinsonian patients
VL  - 36
SP  - 339
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_farfar_520
ER  - 

@conference{
author = "Pokrajac, Milena and Kovacević, I and Miljković, Branislava and Mijajlović, M and Džoljić, Eleonora and Kostić, V",
year = "2004",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Drug Metabolism Reviews",
title = "The monitoring of fluoxetine/norfluoxetine plasma levels for rational use of fluoxetine in depressed Parkinsonian patients",
volume = "36",
pages = "339-339",
url = "https://hdl.handle.net/21.15107/rcub_farfar_520"
}

Pokrajac, M., Kovacević, I., Miljković, B., Mijajlović, M., Džoljić, E.,& Kostić, V.. (2004). The monitoring of fluoxetine/norfluoxetine plasma levels for rational use of fluoxetine in depressed Parkinsonian patients. in Drug Metabolism Reviews
Taylor & Francis Inc, Philadelphia., 36, 339-339.
https://hdl.handle.net/21.15107/rcub_farfar_520

Pokrajac M, Kovacević I, Miljković B, Mijajlović M, Džoljić E, Kostić V. The monitoring of fluoxetine/norfluoxetine plasma levels for rational use of fluoxetine in depressed Parkinsonian patients. in Drug Metabolism Reviews. 2004;36:339-339.
https://hdl.handle.net/21.15107/rcub_farfar_520 .

Pokrajac, Milena, Kovacević, I, Miljković, Branislava, Mijajlović, M, Džoljić, Eleonora, Kostić, V, "The monitoring of fluoxetine/norfluoxetine plasma levels for rational use of fluoxetine in depressed Parkinsonian patients" in Drug Metabolism Reviews, 36 (2004):339-339,
https://hdl.handle.net/21.15107/rcub_farfar_520 .

TY  - JOUR
AU  - Miljković, Branislava
AU  - Brzaković, B
AU  - Kovacević, I
AU  - Agbaba, Danica
AU  - Pokrajac, Milena
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/419
AB  - A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.
PB  - Research Inst Medicinal Plants, Budakalasz
T2  - Journal of Planar Chromatography - Modern TLC
T1  - Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man
VL  - 16
IS  - 3
SP  - 211
EP  - 213
DO  - 10.1556/JPC.16.2003.3.8
ER  - 

@article{
author = "Miljković, Branislava and Brzaković, B and Kovacević, I and Agbaba, Danica and Pokrajac, Milena",
year = "2003",
abstract = "A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.",
publisher = "Research Inst Medicinal Plants, Budakalasz",
journal = "Journal of Planar Chromatography - Modern TLC",
title = "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man",
volume = "16",
number = "3",
pages = "211-213",
doi = "10.1556/JPC.16.2003.3.8"
}

Miljković, B., Brzaković, B., Kovacević, I., Agbaba, D.,& Pokrajac, M.. (2003). Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC
Research Inst Medicinal Plants, Budakalasz., 16(3), 211-213.
https://doi.org/10.1556/JPC.16.2003.3.8

Miljković B, Brzaković B, Kovacević I, Agbaba D, Pokrajac M. Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC. 2003;16(3):211-213.
doi:10.1556/JPC.16.2003.3.8 .

Miljković, Branislava, Brzaković, B, Kovacević, I, Agbaba, Danica, Pokrajac, Milena, "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man" in Journal of Planar Chromatography - Modern TLC, 16, no. 3 (2003):211-213,
https://doi.org/10.1556/JPC.16.2003.3.8 . .