TY  - JOUR
AU  - Aleksić, Mara
AU  - Kapetanović, Vera
AU  - Atanacković, Jasmina
AU  - Jocić, Biljana
AU  - Zečević, Mira
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1032
AB  - Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.
PB  - Elsevier Science BV, Amsterdam
T2  - Talanta
T1  - Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods
VL  - 77
IS  - 1
SP  - 131
EP  - 137
DO  - 10.1016/j.talanta.2008.05.047
ER  - 

@article{
author = "Aleksić, Mara and Kapetanović, Vera and Atanacković, Jasmina and Jocić, Biljana and Zečević, Mira",
year = "2008",
abstract = "Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods",
volume = "77",
number = "1",
pages = "131-137",
doi = "10.1016/j.talanta.2008.05.047"
}

Aleksić, M., Kapetanović, V., Atanacković, J., Jocić, B.,& Zečević, M.. (2008). Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta
Elsevier Science BV, Amsterdam., 77(1), 131-137.
https://doi.org/10.1016/j.talanta.2008.05.047

Aleksić M, Kapetanović V, Atanacković J, Jocić B, Zečević M. Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta. 2008;77(1):131-137.
doi:10.1016/j.talanta.2008.05.047 .

Aleksić, Mara, Kapetanović, Vera, Atanacković, Jasmina, Jocić, Biljana, Zečević, Mira, "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods" in Talanta, 77, no. 1 (2008):131-137,
https://doi.org/10.1016/j.talanta.2008.05.047 . .

TY  - JOUR
AU  - Ilić, Miloš
AU  - Atanacković, Jasmina
AU  - Kapetanović, Vera
AU  - Aleksić, Mara
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/911
AB  - There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC.
AB  - Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Analytical methods for determination of cephalosporins in cerebrospinal fluid
T1  - Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti
VL  - 57
IS  - 3
SP  - 164
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_farfar_911
ER  - 

@article{
author = "Ilić, Miloš and Atanacković, Jasmina and Kapetanović, Vera and Aleksić, Mara",
year = "2007",
abstract = "There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC., Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Analytical methods for determination of cephalosporins in cerebrospinal fluid, Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti",
volume = "57",
number = "3",
pages = "164-177",
url = "https://hdl.handle.net/21.15107/rcub_farfar_911"
}

Ilić, M., Atanacković, J., Kapetanović, V.,& Aleksić, M.. (2007). Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 57(3), 164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911

Ilić M, Atanacković J, Kapetanović V, Aleksić M. Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju. 2007;57(3):164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911 .

Ilić, Miloš, Atanacković, Jasmina, Kapetanović, Vera, Aleksić, Mara, "Analytical methods for determination of cephalosporins in cerebrospinal fluid" in Arhiv za farmaciju, 57, no. 3 (2007):164-177,
https://hdl.handle.net/21.15107/rcub_farfar_911 .