TY  - JOUR
AU  - Ilić, Miloš
AU  - Atanacković, Jasmina
AU  - Kapetanović, Vera
AU  - Aleksić, Mara
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/911
AB  - There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC.
AB  - Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Analytical methods for determination of cephalosporins in cerebrospinal fluid
T1  - Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti
VL  - 57
IS  - 3
SP  - 164
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_farfar_911
ER  - 

@article{
author = "Ilić, Miloš and Atanacković, Jasmina and Kapetanović, Vera and Aleksić, Mara",
year = "2007",
abstract = "There are a lot of papers describing cephalosporin determination in biological samples, like plasma, urine and serum, but very few of them dealing with their determination in cerebrospinal fluids (CSF). The reason for this is probably the low cephalosporin concentration in CSF and difficulties with sample collection. This paper presents review of the articles dealing with determination of cephalosporins in the cerebrospinal fluid, published in last ten years. The aim of this article is to compare available analytical methods used for these determinations. There are several analytical methods that are used for cephalosporin determination in CSF, such as highperformance liquid chromatography (HPLC), capillary electrophoresis (capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC)) and adsorptive stripping voltammetry (AdSV). Cefepime, ceftriaxone, cefuroxime, cefotaxime, cefixime and ceftazidime are cephalosporins already investigated and determined in CSF using above mentioned techniques. The lowest limit of detection (2,3 × 10-4 μg/mL) and limit of quantification (7,68 × 10-4 μg/mL) for cephalosporins in cerebrospinal fluid were determined for cefepime using Adsorptive "Stripping" Voltammetry. Other techiniques showed lower sensitivity, with LOD values in the range from 0.08 μg/mL to 0.2 μg/mL for HPLC, and above 0.3 μg/mL in the case of CZE and MEKC., Većina objavljenih radova bavi se određivanjem cefalosporina u biološkom materijalu, poput plazme, urina i seruma, ali je istovremeno malo radova u kojima je opisano njihovo određivanje u cerebrospinalnoj tečnosti, verovatno zbog male koncentracije cefalosporina u ovom fluidu i njegovog težeg uzorkovanja. Ovaj rad predstavlja pregled rezultata određivanja cefalosporina u cerebrospinalnoj tečnosti objavljenih u poslednjih desetak godina i istovremeno vrši uporednu analizu analitičkih metoda koje se koriste za ova merenja. Metode koje se koriste za analitičko određivanje ovih antibiotika u cerebrospinalnoj tečnosti su tečna hromatografija pod visokim pritiskom (HPLC), kapilarna elektroforeza (kapilarna zonska elektroforeza (CZE), micelarna elektrokinetička kapilarna hromatografija (MEKC)), kao i adsorptivna "stripping" voltametrija (AdSV). Cefalosporini koji su ispitani i određeni korišćenjem navedenih metoda su cefepim (HPLC, MEKC, AdSV), ceftriakson i cefotaksim (CZE, HPLC), cefiksim (HPLC), cefuroksim i ceftazidim (CZE). Najniže vrednosti granice detekcije (LOD) i granice određivanja (LOQ) cefalosporina u cerebrospinalnoj tečnosti dobijene su za cefepim i to metodom adsorptivne "stripping" voltametrije i iznose 2,3 × 10-4 μg/mL i 7,68 × 10-4 μg/mL, respektivno. Ostale razmatrane metode pokazale su manju osetljivost, kod HPLC vrednost LOD se kretala od 0,08 μg/mL do 0,2 μg/mL, a kod CZE i MEKC granica detekcije pojedinih cefalosporina iznosila je preko 0,3 μg/mL.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Analytical methods for determination of cephalosporins in cerebrospinal fluid, Analitičke metode određivanja cefalosporina u cerebrospinalnoj tečnosti",
volume = "57",
number = "3",
pages = "164-177",
url = "https://hdl.handle.net/21.15107/rcub_farfar_911"
}

Ilić, M., Atanacković, J., Kapetanović, V.,& Aleksić, M.. (2007). Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 57(3), 164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911

Ilić M, Atanacković J, Kapetanović V, Aleksić M. Analytical methods for determination of cephalosporins in cerebrospinal fluid. in Arhiv za farmaciju. 2007;57(3):164-177.
https://hdl.handle.net/21.15107/rcub_farfar_911 .

Ilić, Miloš, Atanacković, Jasmina, Kapetanović, Vera, Aleksić, Mara, "Analytical methods for determination of cephalosporins in cerebrospinal fluid" in Arhiv za farmaciju, 57, no. 3 (2007):164-177,
https://hdl.handle.net/21.15107/rcub_farfar_911 .

TY  - JOUR
AU  - Aleksić, Mara
AU  - Ilić, Miloš
AU  - Kapetanović, Vera
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5467
AB  - Abstract
It was found that the reduction of the cefpodoxime proxetil (CP) molecule is strongly influenced by the adsorption. The adsorptive
properties of CP were investigated in order to achieve an increase sensitivity of its determination. Validated adsorptive stripping differential
pulse voltammetry is applied for the determination of low concentration of CP at pH 3.5 and 9.0 where the best pronounced adsorption effects
were observed. The linearity of the calibration curves were achieved from 1 × 10−8 to 1 × 10−7 M with limit of detection (LOD) of 6.3×10−9
and 7.1 × 10−9 M, and limit of quantification (LOQ) of 2.1 × 10−8 and 2.3 × 10−8 M, at pH 3.5 and 9.0, respectively. The proposed method
was tested for CP determination in spiked urine samples, enabling determination of low concentrations of CP.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical  Analysis
T1  - Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine
VL  - 36
IS  - 4
SP  - 899
EP  - 993
DO  - 10.1016/j.jpba.2004.08.035
ER  - 

@article{
author = "Aleksić, Mara and Ilić, Miloš and Kapetanović, Vera",
year = "2004",
abstract = "Abstract
It was found that the reduction of the cefpodoxime proxetil (CP) molecule is strongly influenced by the adsorption. The adsorptive
properties of CP were investigated in order to achieve an increase sensitivity of its determination. Validated adsorptive stripping differential
pulse voltammetry is applied for the determination of low concentration of CP at pH 3.5 and 9.0 where the best pronounced adsorption effects
were observed. The linearity of the calibration curves were achieved from 1 × 10−8 to 1 × 10−7 M with limit of detection (LOD) of 6.3×10−9
and 7.1 × 10−9 M, and limit of quantification (LOQ) of 2.1 × 10−8 and 2.3 × 10−8 M, at pH 3.5 and 9.0, respectively. The proposed method
was tested for CP determination in spiked urine samples, enabling determination of low concentrations of CP.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical  Analysis",
title = "Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine",
volume = "36",
number = "4",
pages = "899-993",
doi = "10.1016/j.jpba.2004.08.035"
}

Aleksić, M., Ilić, M.,& Kapetanović, V.. (2004). Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine. in Journal of Pharmaceutical and Biomedical  Analysis
Elsevier B.V.., 36(4), 899-993.
https://doi.org/10.1016/j.jpba.2004.08.035

Aleksić M, Ilić M, Kapetanović V. Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine. in Journal of Pharmaceutical and Biomedical  Analysis. 2004;36(4):899-993.
doi:10.1016/j.jpba.2004.08.035 .

Aleksić, Mara, Ilić, Miloš, Kapetanović, Vera, "Adsorptive properties of cefpodoxime proxetil as a tool for a new method of its determination in urine" in Journal of Pharmaceutical and Biomedical  Analysis, 36, no. 4 (2004):899-993,
https://doi.org/10.1016/j.jpba.2004.08.035 . .