TY  - JOUR
AU  - Pavlović, Bojana
AU  - Cvijetić, Nataša
AU  - Dragacević, Luka
AU  - Ivković, Branka
AU  - Vujić, Zorica
AU  - Kuntić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2597
AB  - One of the most commonly used surfactants in the production of split virus influenza vaccine is nonionic surfactant Triton X-100. After splitting of the virus is accomplished, Triton X-100 is removed from the vaccine by subsequent production steps. Because of toxicity of Triton X-100, which remains in the vaccine in residual amounts, a sufficiently sensitive method for its detection and quantification needs to be defined. Two methods for determination of Triton X-100 residuals were developed: the UV-spectrophotometry and HPLC methods. For both methods, preparation of vaccine samples and removal of proteins and virus particles were crucial: samples were treated with methanol (1:1) and then centrifuged at 25000 x g for 30 min. After such treatment, the majority of vaccine components that interfered in the UV region were removed, and diluted samples could be directly measured. The chromatographic system included C18 column, step methanol gradient, and detection at 225 nm with a single peak of Triton X-100 at 12.6 min. Both methods were validated and gave satisfactory results for accuracy, precision, specificity, linearity, and robustness. LOQ was slightly lower for the HPLC method. Hence, it was shown that both methods are suitable for analysis of residual amounts of Triton X-100, with the advantages of the UV method being its simplicity and availability in most laboratories.
PB  - AOAC Int, Gaithersburg
T2  - Journal of AOAC International
T1  - Direct UV Spectrophotometry and HPLC Determination of Triton X-100 in Split Virus Influenza Vaccine
VL  - 99
IS  - 2
SP  - 396
EP  - 400
DO  - 10.5740/jaoacint.15-0201
ER  - 

@article{
author = "Pavlović, Bojana and Cvijetić, Nataša and Dragacević, Luka and Ivković, Branka and Vujić, Zorica and Kuntić, Vesna",
year = "2016",
abstract = "One of the most commonly used surfactants in the production of split virus influenza vaccine is nonionic surfactant Triton X-100. After splitting of the virus is accomplished, Triton X-100 is removed from the vaccine by subsequent production steps. Because of toxicity of Triton X-100, which remains in the vaccine in residual amounts, a sufficiently sensitive method for its detection and quantification needs to be defined. Two methods for determination of Triton X-100 residuals were developed: the UV-spectrophotometry and HPLC methods. For both methods, preparation of vaccine samples and removal of proteins and virus particles were crucial: samples were treated with methanol (1:1) and then centrifuged at 25000 x g for 30 min. After such treatment, the majority of vaccine components that interfered in the UV region were removed, and diluted samples could be directly measured. The chromatographic system included C18 column, step methanol gradient, and detection at 225 nm with a single peak of Triton X-100 at 12.6 min. Both methods were validated and gave satisfactory results for accuracy, precision, specificity, linearity, and robustness. LOQ was slightly lower for the HPLC method. Hence, it was shown that both methods are suitable for analysis of residual amounts of Triton X-100, with the advantages of the UV method being its simplicity and availability in most laboratories.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "Direct UV Spectrophotometry and HPLC Determination of Triton X-100 in Split Virus Influenza Vaccine",
volume = "99",
number = "2",
pages = "396-400",
doi = "10.5740/jaoacint.15-0201"
}

Pavlović, B., Cvijetić, N., Dragacević, L., Ivković, B., Vujić, Z.,& Kuntić, V.. (2016). Direct UV Spectrophotometry and HPLC Determination of Triton X-100 in Split Virus Influenza Vaccine. in Journal of AOAC International
AOAC Int, Gaithersburg., 99(2), 396-400.
https://doi.org/10.5740/jaoacint.15-0201

Pavlović B, Cvijetić N, Dragacević L, Ivković B, Vujić Z, Kuntić V. Direct UV Spectrophotometry and HPLC Determination of Triton X-100 in Split Virus Influenza Vaccine. in Journal of AOAC International. 2016;99(2):396-400.
doi:10.5740/jaoacint.15-0201 .

Pavlović, Bojana, Cvijetić, Nataša, Dragacević, Luka, Ivković, Branka, Vujić, Zorica, Kuntić, Vesna, "Direct UV Spectrophotometry and HPLC Determination of Triton X-100 in Split Virus Influenza Vaccine" in Journal of AOAC International, 99, no. 2 (2016):396-400,
https://doi.org/10.5740/jaoacint.15-0201 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Brborić, Jasmina
AU  - Vujić, Zorica
AU  - Uskoković-Marković, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2718
AB  - Many of modern diagnostic methods cannot be achieved without radioisotopes. In this review, an overview of the most common radioisotopes (radionuclides) used as markers for in vitro and in vivo studies is provided. To determine the extremely low concentrations of some biological molecules (hormones, drugs, toxins, nucleic acid, etc.) in biological fluids for in vitro studies, β and/or γ emitters with different half-lives: H-3, C-14, P-32, P-33, I-125, Cr-51 were frequently used. The majority of all radionuclides (90 %) were utilized for in vivo diagnostics. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are imaging modalities widely used in diagnostic nuclear medicine. The most important diagnostic radionuclides are technetium-99m (for single photon emission computed tomography) and fluorine-18 (for positron emission tomography).
PB  - Chemical Publishing Co.
T2  - Asian Journal of Chemistry
T1  - Radioisotopes used as radiotracers for in vitro and in vivo diagnostics
VL  - 28
IS  - 2
SP  - 235
EP  - 241
DO  - 10.14233/ajchem.2016.19401
ER  - 

@article{
author = "Kuntić, Vesna and Brborić, Jasmina and Vujić, Zorica and Uskoković-Marković, Snežana",
year = "2016",
abstract = "Many of modern diagnostic methods cannot be achieved without radioisotopes. In this review, an overview of the most common radioisotopes (radionuclides) used as markers for in vitro and in vivo studies is provided. To determine the extremely low concentrations of some biological molecules (hormones, drugs, toxins, nucleic acid, etc.) in biological fluids for in vitro studies, β and/or γ emitters with different half-lives: H-3, C-14, P-32, P-33, I-125, Cr-51 were frequently used. The majority of all radionuclides (90 %) were utilized for in vivo diagnostics. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are imaging modalities widely used in diagnostic nuclear medicine. The most important diagnostic radionuclides are technetium-99m (for single photon emission computed tomography) and fluorine-18 (for positron emission tomography).",
publisher = "Chemical Publishing Co.",
journal = "Asian Journal of Chemistry",
title = "Radioisotopes used as radiotracers for in vitro and in vivo diagnostics",
volume = "28",
number = "2",
pages = "235-241",
doi = "10.14233/ajchem.2016.19401"
}

Kuntić, V., Brborić, J., Vujić, Z.,& Uskoković-Marković, S.. (2016). Radioisotopes used as radiotracers for in vitro and in vivo diagnostics. in Asian Journal of Chemistry
Chemical Publishing Co.., 28(2), 235-241.
https://doi.org/10.14233/ajchem.2016.19401

Kuntić V, Brborić J, Vujić Z, Uskoković-Marković S. Radioisotopes used as radiotracers for in vitro and in vivo diagnostics. in Asian Journal of Chemistry. 2016;28(2):235-241.
doi:10.14233/ajchem.2016.19401 .

Kuntić, Vesna, Brborić, Jasmina, Vujić, Zorica, Uskoković-Marković, Snežana, "Radioisotopes used as radiotracers for in vitro and in vivo diagnostics" in Asian Journal of Chemistry, 28, no. 2 (2016):235-241,
https://doi.org/10.14233/ajchem.2016.19401 . .

TY  - JOUR
AU  - Smajić, Miralem
AU  - Nikolić, Katarina
AU  - Vujić, Zorica
AU  - Ahmetović, Lejla
AU  - Kuntić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2574
AB  - A 3D-QSAR model using the GRIND/ALMOND descriptors has been performed on a set of 49 angiotensin receptor blockers, also known as angiotensin II receptor antagonists, a family of agents that bind to and inhibit the angiotensin II type 1 receptor. The most commonly used chemical probes: DRY (hydrophobic interaction), O (carbonyl oxygen sp(2), hydrogen bond donor), N1 (NH neutral, hydrogen bond acceptor) and TIP (shape descriptor molecular forms) were derived from the GRID molecular interaction fields. A statistical approach was undertaken using the method of partial least squares within the Pentacle program. The results show satisfactory accuracy of the prediction model (RMSEE = 0.239, R-2 = 0.94, Q(2) = 0.85). The V597 (DRY-TIP) and V763 (O-TIP) represent the most significant variables that correlate positively with the activity of the ARBs. Thirty novel structures of ARBs were designed, according to the developed 3D-QSAR model and pharmacophore, what might set the basis for development of new antihypertensive drugs.
PB  - Springer Birkhauser, New York
T2  - Medicinal Chemistry Research
T1  - 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists
VL  - 25
IS  - 1
SP  - 51
EP  - 61
DO  - 10.1007/s00044-015-1470-1
ER  - 

@article{
author = "Smajić, Miralem and Nikolić, Katarina and Vujić, Zorica and Ahmetović, Lejla and Kuntić, Vesna",
year = "2016",
abstract = "A 3D-QSAR model using the GRIND/ALMOND descriptors has been performed on a set of 49 angiotensin receptor blockers, also known as angiotensin II receptor antagonists, a family of agents that bind to and inhibit the angiotensin II type 1 receptor. The most commonly used chemical probes: DRY (hydrophobic interaction), O (carbonyl oxygen sp(2), hydrogen bond donor), N1 (NH neutral, hydrogen bond acceptor) and TIP (shape descriptor molecular forms) were derived from the GRID molecular interaction fields. A statistical approach was undertaken using the method of partial least squares within the Pentacle program. The results show satisfactory accuracy of the prediction model (RMSEE = 0.239, R-2 = 0.94, Q(2) = 0.85). The V597 (DRY-TIP) and V763 (O-TIP) represent the most significant variables that correlate positively with the activity of the ARBs. Thirty novel structures of ARBs were designed, according to the developed 3D-QSAR model and pharmacophore, what might set the basis for development of new antihypertensive drugs.",
publisher = "Springer Birkhauser, New York",
journal = "Medicinal Chemistry Research",
title = "3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists",
volume = "25",
number = "1",
pages = "51-61",
doi = "10.1007/s00044-015-1470-1"
}

Smajić, M., Nikolić, K., Vujić, Z., Ahmetović, L.,& Kuntić, V.. (2016). 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists. in Medicinal Chemistry Research
Springer Birkhauser, New York., 25(1), 51-61.
https://doi.org/10.1007/s00044-015-1470-1

Smajić M, Nikolić K, Vujić Z, Ahmetović L, Kuntić V. 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists. in Medicinal Chemistry Research. 2016;25(1):51-61.
doi:10.1007/s00044-015-1470-1 .

Smajić, Miralem, Nikolić, Katarina, Vujić, Zorica, Ahmetović, Lejla, Kuntić, Vesna, "3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists" in Medicinal Chemistry Research, 25, no. 1 (2016):51-61,
https://doi.org/10.1007/s00044-015-1470-1 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Brborić, Jasmina
AU  - Holclajtner-Antunović, Ivanka
AU  - Uskoković-Marković, Snežana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2256
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey
T1  - Ocena bioaktivnih efekata flavonoida hesperidina - pregled podataka iz novije literature
VL  - 71
IS  - 1
SP  - 60
EP  - 65
DO  - 10.2298/VSP1401060K
ER  - 

@article{
author = "Kuntić, Vesna and Brborić, Jasmina and Holclajtner-Antunović, Ivanka and Uskoković-Marković, Snežana",
year = "2014",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey, Ocena bioaktivnih efekata flavonoida hesperidina - pregled podataka iz novije literature",
volume = "71",
number = "1",
pages = "60-65",
doi = "10.2298/VSP1401060K"
}

Kuntić, V., Brborić, J., Holclajtner-Antunović, I.,& Uskoković-Marković, S.. (2014). Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(1), 60-65.
https://doi.org/10.2298/VSP1401060K

Kuntić V, Brborić J, Holclajtner-Antunović I, Uskoković-Marković S. Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey. in Vojnosanitetski pregled. 2014;71(1):60-65.
doi:10.2298/VSP1401060K .

Kuntić, Vesna, Brborić, Jasmina, Holclajtner-Antunović, Ivanka, Uskoković-Marković, Snežana, "Evaluating the bioactive effects of flavonoid hesperidin: A new literature data survey" in Vojnosanitetski pregled, 71, no. 1 (2014):60-65,
https://doi.org/10.2298/VSP1401060K . .

TY  - JOUR
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Brborić, Jasmina
AU  - Kuntić, Vesna
AU  - Uskoković-Marković, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1928
AB  - The aim of the present study was to optimize a chromatographic method for the analysis of atorvastatin (acid and lactone forms), ortho- and para-hydroxyatorvastatin by using an experimental design approach. Optimization experiments were conducted through a process of screening and optimization. The purpose of a screening design is to identify the factors that have significant effects on the selected chromatographic responses, and for this purpose a full 23 factorial design was used. The location of the true optimum was established by applying Derringer's desirability function, which provides simultaneously optimization of all seven responses. The ranges of the independent variables used for the optimization were content of acetonitrile in mobile phase (60-70%), temperature of column (30-40 C degrees) and flow rate (0.8-1.2 mL min(-1)). The influences of these independent variables were evaluated for the output responses: retention time of first peak (p-hydroxyatorvastatin) and of last peak (atorvastatin, lactone form), symmetries of all four peaks and relative retention time of p-hydroxyatorvastatin. The primary goal of this investigation was establishing a new simple and sensitive method that could be used in analysis of biological samples. The method was validated and successfully applied for determination of atorvastatin (acid and lactone forms) and its metabolites in plasma.
PB  - MDPI, Basel
T2  - Molecules
T1  - An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma
VL  - 18
IS  - 3
SP  - 2469
EP  - 2482
DO  - 10.3390/molecules18032469
ER  - 

@article{
author = "Crevar-Sakač, Milkica and Vujić, Zorica and Brborić, Jasmina and Kuntić, Vesna and Uskoković-Marković, Snežana",
year = "2013",
abstract = "The aim of the present study was to optimize a chromatographic method for the analysis of atorvastatin (acid and lactone forms), ortho- and para-hydroxyatorvastatin by using an experimental design approach. Optimization experiments were conducted through a process of screening and optimization. The purpose of a screening design is to identify the factors that have significant effects on the selected chromatographic responses, and for this purpose a full 23 factorial design was used. The location of the true optimum was established by applying Derringer's desirability function, which provides simultaneously optimization of all seven responses. The ranges of the independent variables used for the optimization were content of acetonitrile in mobile phase (60-70%), temperature of column (30-40 C degrees) and flow rate (0.8-1.2 mL min(-1)). The influences of these independent variables were evaluated for the output responses: retention time of first peak (p-hydroxyatorvastatin) and of last peak (atorvastatin, lactone form), symmetries of all four peaks and relative retention time of p-hydroxyatorvastatin. The primary goal of this investigation was establishing a new simple and sensitive method that could be used in analysis of biological samples. The method was validated and successfully applied for determination of atorvastatin (acid and lactone forms) and its metabolites in plasma.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma",
volume = "18",
number = "3",
pages = "2469-2482",
doi = "10.3390/molecules18032469"
}

Crevar-Sakač, M., Vujić, Z., Brborić, J., Kuntić, V.,& Uskoković-Marković, S.. (2013). An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma. in Molecules
MDPI, Basel., 18(3), 2469-2482.
https://doi.org/10.3390/molecules18032469

Crevar-Sakač M, Vujić Z, Brborić J, Kuntić V, Uskoković-Marković S. An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma. in Molecules. 2013;18(3):2469-2482.
doi:10.3390/molecules18032469 .

Crevar-Sakač, Milkica, Vujić, Zorica, Brborić, Jasmina, Kuntić, Vesna, Uskoković-Marković, Snežana, "An Improved HPLC Method with the Aid of a Chemometric Protocol: Simultaneous Determination of Atorvastatin and Its Metabolites in Plasma" in Molecules, 18, no. 3 (2013):2469-2482,
https://doi.org/10.3390/molecules18032469 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Stanković, Miroslava
AU  - Vujić, Zorica
AU  - Brborić, Jasmina
AU  - Uskoković-Marković, Snežana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1910
AB  - To protect organisms from ionizing radiation (IR), and to reduce morbidity or mortality, various agents, called radioprotectors, have been utilized. Because radiation-induced cellular damage is attributed primarily to the harmful effects of free radicals, molecules with radical-scavenging properties are particularly promising as radioprotectors. Early development of such agents focused on thiol synthetic compounds, known as WR protectors, but only amifostine (WR-2721) has been used in clinical trials as an officially approved radioprotector. Besides thiol compounds, various compounds with different chemical structure were investigated, but an ideal radioprotector has not been found yet. Plants and natural products have been evaluated as promising sources of radioprotectors because of their low toxicity, although they exhibit an inferior protection level compared to synthetic thiol compounds. Active plant constituents seem to exert the radioprotection through antioxidant and free radical-scavenging activities. Our research established that plants containing polyphenolic compounds (raspberry, blueberry, strawberry, grape, etc.) exhibit antioxidative activities and protect genetic material from IR.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Radioprotectors - the Evergreen Topic
VL  - 10
IS  - 10
SP  - 1791
EP  - 1803
DO  - 10.1002/cbdv.201300054
ER  - 

@article{
author = "Kuntić, Vesna and Stanković, Miroslava and Vujić, Zorica and Brborić, Jasmina and Uskoković-Marković, Snežana",
year = "2013",
abstract = "To protect organisms from ionizing radiation (IR), and to reduce morbidity or mortality, various agents, called radioprotectors, have been utilized. Because radiation-induced cellular damage is attributed primarily to the harmful effects of free radicals, molecules with radical-scavenging properties are particularly promising as radioprotectors. Early development of such agents focused on thiol synthetic compounds, known as WR protectors, but only amifostine (WR-2721) has been used in clinical trials as an officially approved radioprotector. Besides thiol compounds, various compounds with different chemical structure were investigated, but an ideal radioprotector has not been found yet. Plants and natural products have been evaluated as promising sources of radioprotectors because of their low toxicity, although they exhibit an inferior protection level compared to synthetic thiol compounds. Active plant constituents seem to exert the radioprotection through antioxidant and free radical-scavenging activities. Our research established that plants containing polyphenolic compounds (raspberry, blueberry, strawberry, grape, etc.) exhibit antioxidative activities and protect genetic material from IR.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Radioprotectors - the Evergreen Topic",
volume = "10",
number = "10",
pages = "1791-1803",
doi = "10.1002/cbdv.201300054"
}

Kuntić, V., Stanković, M., Vujić, Z., Brborić, J.,& Uskoković-Marković, S.. (2013). Radioprotectors - the Evergreen Topic. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 10(10), 1791-1803.
https://doi.org/10.1002/cbdv.201300054

Kuntić V, Stanković M, Vujić Z, Brborić J, Uskoković-Marković S. Radioprotectors - the Evergreen Topic. in Chemistry & Biodiversity. 2013;10(10):1791-1803.
doi:10.1002/cbdv.201300054 .

Kuntić, Vesna, Stanković, Miroslava, Vujić, Zorica, Brborić, Jasmina, Uskoković-Marković, Snežana, "Radioprotectors - the Evergreen Topic" in Chemistry & Biodiversity, 10, no. 10 (2013):1791-1803,
https://doi.org/10.1002/cbdv.201300054 . .

TY  - JOUR
AU  - Bazov, Igor
AU  - Kononenko, Olga
AU  - Watanabe, Hiroyuki
AU  - Kuntić, Vesna
AU  - Sarkisyan, Daniil
AU  - Taqi, Malik M.
AU  - Hussain, Muhammad Z.
AU  - Nyberg, Fred
AU  - Yakovleva, Tatjana
AU  - Bakalkin, Georgy
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1942
AB  - The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular dysregulations in this system may be specific for different stages of addiction cycle and neurocircuitries involved and therefore may differentially contribute to the initiation and maintenance of addiction. Here we evaluated whether the EOS is altered in brain areas involved in cognitive control of addiction including the dorsolateral prefrontal cortex (dl-PFC), orbitofrontal cortex (OFC) and hippocampus in human alcohol-dependent subjects. Levels of EOS mRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and levels of dynorphins by radioimmunoassay (RIA) in post-mortem specimens obtained from 14 alcoholics and 14 controls. Prodynorphin mRNA and dynorphins in dl-PFC, ?-opioid receptor mRNA in OFC and dynorphins in hippocampus were up-regulated in alcoholics. No significant changes in expression of proenkephalin, and mu- and d-opioid receptors were evident; pro-opiomelanocortin mRNA levels were below the detection limit. Activation of the ?-opioid receptor by up-regulated dynorphins in alcoholics may underlie in part neurocognitive dysfunctions relevant for addiction and disrupted inhibitory control.
PB  - Wiley-Blackwell, Hoboken
T2  - Addiction Biology
T1  - The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction
VL  - 18
IS  - 1
SP  - 161
EP  - 169
DO  - 10.1111/j.1369-1600.2011.00366.x
ER  - 

@article{
author = "Bazov, Igor and Kononenko, Olga and Watanabe, Hiroyuki and Kuntić, Vesna and Sarkisyan, Daniil and Taqi, Malik M. and Hussain, Muhammad Z. and Nyberg, Fred and Yakovleva, Tatjana and Bakalkin, Georgy",
year = "2013",
abstract = "The endogenous opioid system (EOS) plays a critical role in addictive processes. Molecular dysregulations in this system may be specific for different stages of addiction cycle and neurocircuitries involved and therefore may differentially contribute to the initiation and maintenance of addiction. Here we evaluated whether the EOS is altered in brain areas involved in cognitive control of addiction including the dorsolateral prefrontal cortex (dl-PFC), orbitofrontal cortex (OFC) and hippocampus in human alcohol-dependent subjects. Levels of EOS mRNAs were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and levels of dynorphins by radioimmunoassay (RIA) in post-mortem specimens obtained from 14 alcoholics and 14 controls. Prodynorphin mRNA and dynorphins in dl-PFC, ?-opioid receptor mRNA in OFC and dynorphins in hippocampus were up-regulated in alcoholics. No significant changes in expression of proenkephalin, and mu- and d-opioid receptors were evident; pro-opiomelanocortin mRNA levels were below the detection limit. Activation of the ?-opioid receptor by up-regulated dynorphins in alcoholics may underlie in part neurocognitive dysfunctions relevant for addiction and disrupted inhibitory control.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Addiction Biology",
title = "The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction",
volume = "18",
number = "1",
pages = "161-169",
doi = "10.1111/j.1369-1600.2011.00366.x"
}

Bazov, I., Kononenko, O., Watanabe, H., Kuntić, V., Sarkisyan, D., Taqi, M. M., Hussain, M. Z., Nyberg, F., Yakovleva, T.,& Bakalkin, G.. (2013). The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction. in Addiction Biology
Wiley-Blackwell, Hoboken., 18(1), 161-169.
https://doi.org/10.1111/j.1369-1600.2011.00366.x

Bazov I, Kononenko O, Watanabe H, Kuntić V, Sarkisyan D, Taqi MM, Hussain MZ, Nyberg F, Yakovleva T, Bakalkin G. The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction. in Addiction Biology. 2013;18(1):161-169.
doi:10.1111/j.1369-1600.2011.00366.x .

Bazov, Igor, Kononenko, Olga, Watanabe, Hiroyuki, Kuntić, Vesna, Sarkisyan, Daniil, Taqi, Malik M., Hussain, Muhammad Z., Nyberg, Fred, Yakovleva, Tatjana, Bakalkin, Georgy, "The endogenous opioid system in human alcoholics: molecular adaptations in brain areas involved in cognitive control of addiction" in Addiction Biology, 18, no. 1 (2013):161-169,
https://doi.org/10.1111/j.1369-1600.2011.00366.x . .

TY  - JOUR
AU  - Ražić, Slavica
AU  - Kuntić, Vesna
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1939
AB  - Nine elements (Fe, Cu, Mn, Zn, As, Cd, Sn, Hg, and Pb) were determined in 8 teas and 25 herbal teas widely consumed in Serbia for medical purposes. Green, black, peppermint, chamomile, cranberry, sage, rosehip, uva, senna, marigold, and fennel teas were investigated. Microwave-assisted acid digestion was used for all of the samples, and the element contents were determined by inductively coupled plasma mass spectrometry. From all of the determined microelements, the highest content was that of Mn, 2912.8 and 2541.8 mg/kg of dry matter in the black and green tea, respectively. The contents of toxic elements (As, Cd, and Pb) were below the maximum permissible levels, except for one brand of peppermint tea in which the level of Cd was 8.61 mg/kg, much higher than the allowed 0.3 mg/kg.
PB  - Taylor & Francis Inc, Philadelphia
T2  - International Journal of Food Properties
T1  - Diverse elements in herbal tea products consumed in serbia using inductively coupled plasma mass spectrometry
VL  - 16
IS  - 1
SP  - 1
EP  - 8
DO  - 10.1080/10942912.2010.526273
ER  - 

@article{
author = "Ražić, Slavica and Kuntić, Vesna",
year = "2013",
abstract = "Nine elements (Fe, Cu, Mn, Zn, As, Cd, Sn, Hg, and Pb) were determined in 8 teas and 25 herbal teas widely consumed in Serbia for medical purposes. Green, black, peppermint, chamomile, cranberry, sage, rosehip, uva, senna, marigold, and fennel teas were investigated. Microwave-assisted acid digestion was used for all of the samples, and the element contents were determined by inductively coupled plasma mass spectrometry. From all of the determined microelements, the highest content was that of Mn, 2912.8 and 2541.8 mg/kg of dry matter in the black and green tea, respectively. The contents of toxic elements (As, Cd, and Pb) were below the maximum permissible levels, except for one brand of peppermint tea in which the level of Cd was 8.61 mg/kg, much higher than the allowed 0.3 mg/kg.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "International Journal of Food Properties",
title = "Diverse elements in herbal tea products consumed in serbia using inductively coupled plasma mass spectrometry",
volume = "16",
number = "1",
pages = "1-8",
doi = "10.1080/10942912.2010.526273"
}

Ražić, S.,& Kuntić, V.. (2013). Diverse elements in herbal tea products consumed in serbia using inductively coupled plasma mass spectrometry. in International Journal of Food Properties
Taylor & Francis Inc, Philadelphia., 16(1), 1-8.
https://doi.org/10.1080/10942912.2010.526273

Ražić S, Kuntić V. Diverse elements in herbal tea products consumed in serbia using inductively coupled plasma mass spectrometry. in International Journal of Food Properties. 2013;16(1):1-8.
doi:10.1080/10942912.2010.526273 .

Ražić, Slavica, Kuntić, Vesna, "Diverse elements in herbal tea products consumed in serbia using inductively coupled plasma mass spectrometry" in International Journal of Food Properties, 16, no. 1 (2013):1-8,
https://doi.org/10.1080/10942912.2010.526273 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Micić, Svetlana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1734
AB  - A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations
VL  - 59
IS  - 2
SP  - 436
EP  - 441
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1734
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Micić, Svetlana",
year = "2012",
abstract = "A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations",
volume = "59",
number = "2",
pages = "436-441",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1734"
}

Kuntić, V., Pejić, N.,& Micić, S.. (2012). Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 59(2), 436-441.
https://hdl.handle.net/21.15107/rcub_farfar_1734

Kuntić V, Pejić N, Micić S. Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica. 2012;59(2):436-441.
https://hdl.handle.net/21.15107/rcub_farfar_1734 .

Kuntić, Vesna, Pejić, Nataša, Micić, Svetlana, "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations" in Acta Chimica Slovenica, 59, no. 2 (2012):436-441,
https://hdl.handle.net/21.15107/rcub_farfar_1734 .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Filipović, Ivana
AU  - Vujić, Zorica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1450
AB  - Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 mu M prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.
PB  - MDPI, Basel
T2  - Molecules
T1  - Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays
VL  - 16
IS  - 2
SP  - 1378
EP  - 1388
DO  - 10.3390/molecules16021378
ER  - 

@article{
author = "Kuntić, Vesna and Filipović, Ivana and Vujić, Zorica",
year = "2011",
abstract = "Two flavonoids, rutin and hesperidin, were investigated in vitro for anticoagulant activity through coagulation tests: activated partial thromboplastin time (aPTT), prothrombin time (PT) and thrombin time (TT). Only an ethanolic solution of rutin at the concentration of 830 mu M prolonged aPTT, while TT and PT were unaffected. In order to evaluate whether the prolongation of aPTT was due to the decrease of coagulation factors, the experiment with deficient plasma was performed, showing the effects on factors VIII and IX. Since pharmacological activity of flavonoids is believed to increase when they are coordinated with metal ions, complexes of these flavonoids with Al(III) and Cu(II) ions were also tested. The results showed that complexes significantly prolonged aPTT and had no effects on PT and TT. Assay with deficient plasma (plasma having the investigated factor at less then 1%) revealed that complexes could bind to the coagulation factors, what may lead to a non-specific inhibition and aPTT prolongation. An effort was made to correlate stability of complexes with their anticoagulant properties.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays",
volume = "16",
number = "2",
pages = "1378-1388",
doi = "10.3390/molecules16021378"
}

Kuntić, V., Filipović, I.,& Vujić, Z.. (2011). Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. in Molecules
MDPI, Basel., 16(2), 1378-1388.
https://doi.org/10.3390/molecules16021378

Kuntić V, Filipović I, Vujić Z. Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays. in Molecules. 2011;16(2):1378-1388.
doi:10.3390/molecules16021378 .

Kuntić, Vesna, Filipović, Ivana, Vujić, Zorica, "Effects of Rutin and Hesperidin and their Al(III) and Cu(II) Complexes on in Vitro Plasma Coagulation Assays" in Molecules, 16, no. 2 (2011):1378-1388,
https://doi.org/10.3390/molecules16021378 . .

TY  - JOUR
AU  - Pejović, Gordana
AU  - Otašević, Biljana
AU  - Zečević, Mira
AU  - Kuntić, Vesna
AU  - Vujić, Zorica
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1432
AB  - The present work describes forced degradation study of valdecoxib, under different ICH recommended stress conditions - photolytic degradation, oxidation, hydrolysis in alkaline and acidic conditions as well as its thermal degradation. Stress studies were performed at valdecoxib drug substance and the final dosage form. Chromatographic separations of drug and the degradation products formed under various experimental conditions was successfully achieved on RP-18e Chromolith® performance monolithic column (100 mm × 4.6 mm, macropore size 2 μm, mesopore size 13 nm) with an eluent containing the mixture of methanol and water solution of TEA (1%, pH 7.4) in the ratio 40:60 (v/v) while the column temperature was adjusted to 30°C. Detection was performed using photodiode array detector on several wavelengths in order to ensure the proper insight into drug degradation pathway in case the formed products differ in absorption characteristics.
T2  - Farmaceutyczny Przeglad Naukowy
T1  - Valdecoxib stability properties under forced degradation conditions
VL  - 7
IS  - 9
SP  - 11
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1432
ER  - 

@article{
author = "Pejović, Gordana and Otašević, Biljana and Zečević, Mira and Kuntić, Vesna and Vujić, Zorica",
year = "2010",
abstract = "The present work describes forced degradation study of valdecoxib, under different ICH recommended stress conditions - photolytic degradation, oxidation, hydrolysis in alkaline and acidic conditions as well as its thermal degradation. Stress studies were performed at valdecoxib drug substance and the final dosage form. Chromatographic separations of drug and the degradation products formed under various experimental conditions was successfully achieved on RP-18e Chromolith® performance monolithic column (100 mm × 4.6 mm, macropore size 2 μm, mesopore size 13 nm) with an eluent containing the mixture of methanol and water solution of TEA (1%, pH 7.4) in the ratio 40:60 (v/v) while the column temperature was adjusted to 30°C. Detection was performed using photodiode array detector on several wavelengths in order to ensure the proper insight into drug degradation pathway in case the formed products differ in absorption characteristics.",
journal = "Farmaceutyczny Przeglad Naukowy",
title = "Valdecoxib stability properties under forced degradation conditions",
volume = "7",
number = "9",
pages = "11-17",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1432"
}

Pejović, G., Otašević, B., Zečević, M., Kuntić, V.,& Vujić, Z.. (2010). Valdecoxib stability properties under forced degradation conditions. in Farmaceutyczny Przeglad Naukowy, 7(9), 11-17.
https://hdl.handle.net/21.15107/rcub_farfar_1432

Pejović G, Otašević B, Zečević M, Kuntić V, Vujić Z. Valdecoxib stability properties under forced degradation conditions. in Farmaceutyczny Przeglad Naukowy. 2010;7(9):11-17.
https://hdl.handle.net/21.15107/rcub_farfar_1432 .

Pejović, Gordana, Otašević, Biljana, Zečević, Mira, Kuntić, Vesna, Vujić, Zorica, "Valdecoxib stability properties under forced degradation conditions" in Farmaceutyczny Przeglad Naukowy, 7, no. 9 (2010):11-17,
https://hdl.handle.net/21.15107/rcub_farfar_1432 .

TY  - JOUR
AU  - Ražić, Slavica
AU  - Kuntić, Vesna
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1445
AB  - The metal contents of Fe, Cu, Mn, Zn, As, Cd, Sn, Hg and Pb in the five herbal tea mixtures containing a variety of different plants were determined. Microwave-assisted acid digestion was used for all of the samples, and the element contents were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). A matched calibration curve method was used for the quantification of the analytes. For accuracy assay, SRM 2977, NIST, Mussel Tissue (Organic Contaminants and Trace Elements) was used. The obtained results agreed with the certified values. The concentration of all elements varied among examined herbal tea mixtures and are in the expected intervals. The contents of all investigated toxic elements were below the maximum permissible levels.
AB  - U ovom radu određen je sadržaj metala u pet izabranih mešavina biljnih čajeva. Devet elemenata: Fe, Cu, Mn, Zn, As, Cd, Sn, Hg i Pb su analizirani metodom induktivno spregnute plazme sa masenom spektrometrijom (ICP-MS). Uzorci su pripremani mikrotalasnom digestijom, sadržaj metala određen je primenom eksterne kalibracije, a tačnost metode proverena je pomoću standardnog referentnog materijala (SRM 2977, NIST) i internog standarda. Dobijeni rezultati su u dobroj saglasnosti sa sertifikovanim vrednostima. U zavisnosti od ispitivane mešavine, koncentracije analiziranih elemenata su različite i nalaze se u očekivanim intervalima. Koncentracije svih teških metala su ispod dozvoljene granice.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Determination of metal contents in herbal tea mixtures consumed in Belgrade using Inductively Coupled Plasma Mass Spectrometry
T1  - Određivanje sadržaja metala u mešavinama biljnih čajeva sa tržišta u Beogradu primenom induktivno spregnute plazme sa masenom spektrometrijom
VL  - 60
IS  - 4
SP  - 391
EP  - 398
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1445
ER  - 

@article{
author = "Ražić, Slavica and Kuntić, Vesna",
year = "2010",
abstract = "The metal contents of Fe, Cu, Mn, Zn, As, Cd, Sn, Hg and Pb in the five herbal tea mixtures containing a variety of different plants were determined. Microwave-assisted acid digestion was used for all of the samples, and the element contents were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). A matched calibration curve method was used for the quantification of the analytes. For accuracy assay, SRM 2977, NIST, Mussel Tissue (Organic Contaminants and Trace Elements) was used. The obtained results agreed with the certified values. The concentration of all elements varied among examined herbal tea mixtures and are in the expected intervals. The contents of all investigated toxic elements were below the maximum permissible levels., U ovom radu određen je sadržaj metala u pet izabranih mešavina biljnih čajeva. Devet elemenata: Fe, Cu, Mn, Zn, As, Cd, Sn, Hg i Pb su analizirani metodom induktivno spregnute plazme sa masenom spektrometrijom (ICP-MS). Uzorci su pripremani mikrotalasnom digestijom, sadržaj metala određen je primenom eksterne kalibracije, a tačnost metode proverena je pomoću standardnog referentnog materijala (SRM 2977, NIST) i internog standarda. Dobijeni rezultati su u dobroj saglasnosti sa sertifikovanim vrednostima. U zavisnosti od ispitivane mešavine, koncentracije analiziranih elemenata su različite i nalaze se u očekivanim intervalima. Koncentracije svih teških metala su ispod dozvoljene granice.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Determination of metal contents in herbal tea mixtures consumed in Belgrade using Inductively Coupled Plasma Mass Spectrometry, Određivanje sadržaja metala u mešavinama biljnih čajeva sa tržišta u Beogradu primenom induktivno spregnute plazme sa masenom spektrometrijom",
volume = "60",
number = "4",
pages = "391-398",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1445"
}

Ražić, S.,& Kuntić, V.. (2010). Determination of metal contents in herbal tea mixtures consumed in Belgrade using Inductively Coupled Plasma Mass Spectrometry. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 60(4), 391-398.
https://hdl.handle.net/21.15107/rcub_farfar_1445

Ražić S, Kuntić V. Determination of metal contents in herbal tea mixtures consumed in Belgrade using Inductively Coupled Plasma Mass Spectrometry. in Arhiv za farmaciju. 2010;60(4):391-398.
https://hdl.handle.net/21.15107/rcub_farfar_1445 .

Ražić, Slavica, Kuntić, Vesna, "Determination of metal contents in herbal tea mixtures consumed in Belgrade using Inductively Coupled Plasma Mass Spectrometry" in Arhiv za farmaciju, 60, no. 4 (2010):391-398,
https://hdl.handle.net/21.15107/rcub_farfar_1445 .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Matić, Silvana
AU  - Kuntić, Vesna
AU  - Vladimirov, Sote
AU  - Uskoković-Marković, Snežana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1339
AB  - A method was developed to determine lindane (gamma-HCH), the widely used organochlorine pesticide, and its impurity alpha-HCH in bulk and pharmaceutical products (shampoo, gel and emulsion). The system was able to successfully resolve the lindane peak from alpha-HCH and interfering components. The method displays an excellent linearity over the concentration range 0.5-15.0 mu g mL(-1) for lindane and 0.005-0.15 mu g mL(-1) for alpha-HCH and a precision better than 2.5% from intra- and inter-day analyses. LOQ and LOD were determined to be 0.45 and 0.15 mu g mL(-1) for lindane and 0.005 and 0.002 mu g mL(-1) for alpha-HCH.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - GC-ECD Determination of Lindane and Its Impurity alpha-HCH in Pharmaceutical Products
VL  - 72
IS  - 5-6
SP  - 581
EP  - 584
DO  - 10.1365/s10337-010-1684-9
ER  - 

@article{
author = "Vujić, Zorica and Matić, Silvana and Kuntić, Vesna and Vladimirov, Sote and Uskoković-Marković, Snežana",
year = "2010",
abstract = "A method was developed to determine lindane (gamma-HCH), the widely used organochlorine pesticide, and its impurity alpha-HCH in bulk and pharmaceutical products (shampoo, gel and emulsion). The system was able to successfully resolve the lindane peak from alpha-HCH and interfering components. The method displays an excellent linearity over the concentration range 0.5-15.0 mu g mL(-1) for lindane and 0.005-0.15 mu g mL(-1) for alpha-HCH and a precision better than 2.5% from intra- and inter-day analyses. LOQ and LOD were determined to be 0.45 and 0.15 mu g mL(-1) for lindane and 0.005 and 0.002 mu g mL(-1) for alpha-HCH.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "GC-ECD Determination of Lindane and Its Impurity alpha-HCH in Pharmaceutical Products",
volume = "72",
number = "5-6",
pages = "581-584",
doi = "10.1365/s10337-010-1684-9"
}

Vujić, Z., Matić, S., Kuntić, V., Vladimirov, S.,& Uskoković-Marković, S.. (2010). GC-ECD Determination of Lindane and Its Impurity alpha-HCH in Pharmaceutical Products. in Chromatographia
Springer Heidelberg, Heidelberg., 72(5-6), 581-584.
https://doi.org/10.1365/s10337-010-1684-9

Vujić Z, Matić S, Kuntić V, Vladimirov S, Uskoković-Marković S. GC-ECD Determination of Lindane and Its Impurity alpha-HCH in Pharmaceutical Products. in Chromatographia. 2010;72(5-6):581-584.
doi:10.1365/s10337-010-1684-9 .

Vujić, Zorica, Matić, Silvana, Kuntić, Vesna, Vladimirov, Sote, Uskoković-Marković, Snežana, "GC-ECD Determination of Lindane and Its Impurity alpha-HCH in Pharmaceutical Products" in Chromatographia, 72, no. 5-6 (2010):581-584,
https://doi.org/10.1365/s10337-010-1684-9 . .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Crevar, Milkica
AU  - Obradović, Vladimir
AU  - Kuntić, Vesna
AU  - Uskoković-Marković, Snežana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1160
AB  - A rapid and simple liquid chromatographic method with UV detection has been developed for the determination of hydrochlorothiazide (HCTZ), cilazapril (CL) and its active metabolite cilazaprilat (CLT) in urine. Sample preparation for urine consisted of solid-phase extraction using styrene-divinylbenzene (SDB-2) cartridges. The chromatographic system was a Zorbax Eclipse XDB-C-18 column with a mixture of methanol and 10 mM phosphate buffer, pH 2.3 with gradient (20 to 60% of methanol) as mobile phase at a flow rate of 1.0 mL min(-1). The detection was performed at the wavelength of 206 nm. Enalapril maleat was used as an internal standard. The detector response was linear in the range of 2.4-30.0, 1.6-15.0 and 1.8-20.0 mu g mL(-1) for HCTZ, CL and CLT, respectively. LOQ was determined to be 2.4, 1.6 and 1.8 mu g mL(-1) for HCTZ, CL and CLT, respectively. Both intra- and inter-day precision were within acceptable limits. The method has been applied to urine samples obtained from three hypertensive patients after intake of HCTZ and CL therapeutic dose.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Simultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LC
VL  - 70
IS  - 7-8
SP  - 1221
EP  - 1225
DO  - 10.1365/s10337-009-1286-6
ER  - 

@article{
author = "Vujić, Zorica and Crevar, Milkica and Obradović, Vladimir and Kuntić, Vesna and Uskoković-Marković, Snežana",
year = "2009",
abstract = "A rapid and simple liquid chromatographic method with UV detection has been developed for the determination of hydrochlorothiazide (HCTZ), cilazapril (CL) and its active metabolite cilazaprilat (CLT) in urine. Sample preparation for urine consisted of solid-phase extraction using styrene-divinylbenzene (SDB-2) cartridges. The chromatographic system was a Zorbax Eclipse XDB-C-18 column with a mixture of methanol and 10 mM phosphate buffer, pH 2.3 with gradient (20 to 60% of methanol) as mobile phase at a flow rate of 1.0 mL min(-1). The detection was performed at the wavelength of 206 nm. Enalapril maleat was used as an internal standard. The detector response was linear in the range of 2.4-30.0, 1.6-15.0 and 1.8-20.0 mu g mL(-1) for HCTZ, CL and CLT, respectively. LOQ was determined to be 2.4, 1.6 and 1.8 mu g mL(-1) for HCTZ, CL and CLT, respectively. Both intra- and inter-day precision were within acceptable limits. The method has been applied to urine samples obtained from three hypertensive patients after intake of HCTZ and CL therapeutic dose.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Simultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LC",
volume = "70",
number = "7-8",
pages = "1221-1225",
doi = "10.1365/s10337-009-1286-6"
}

Vujić, Z., Crevar, M., Obradović, V., Kuntić, V.,& Uskoković-Marković, S.. (2009). Simultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LC. in Chromatographia
Springer Heidelberg, Heidelberg., 70(7-8), 1221-1225.
https://doi.org/10.1365/s10337-009-1286-6

Vujić Z, Crevar M, Obradović V, Kuntić V, Uskoković-Marković S. Simultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LC. in Chromatographia. 2009;70(7-8):1221-1225.
doi:10.1365/s10337-009-1286-6 .

Vujić, Zorica, Crevar, Milkica, Obradović, Vladimir, Kuntić, Vesna, Uskoković-Marković, Snežana, "Simultaneous Determination of Hydrochlorothiazide, Cilazapril and Its Active Metabolite Cilazaprilat in Urine by Gradient RP-LC" in Chromatographia, 70, no. 7-8 (2009):1221-1225,
https://doi.org/10.1365/s10337-009-1286-6 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Stojić-Vukanić, Zorica
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1308
AB  - Nephelometry and turbidimetry are methods based on the phenomenon whereby light, passing through a medium with dispersed particles is attenuated in intensity by scattering. The intensity of the scattered light is measured at right angle (usually, but not necessarily) to the incident light beam (nephelometry) or at a forward angle (turbidimetry). Based on nephelometry and turbidimetry, immunonephelometric and immunoturbidimetric assays for the measurement of proteins have developed and expanded rapidly in recent years, progressively replacing the time-honored gel precipitation techniques. In this study, the theoretical background of light scattering theory, instrumental systems for nephelometry and turbidimetry, the nature and kinetics of the antibody-antigen reaction in fluid, and the application of immunonephelometric and immunoturbidimetric assays for specific proteins in the clinical laboratory are described.
AB  - Nefelometrija i turbidimetrija su metode koje sa zasnivaju na rasipanju svetlosti: pri prolasku kroz disperzionu sredinu, svetlost se rasipa na česticama disperzione faze. Intenzitet rasute svetlosti može se meriti pod nekim uglom (uobičajeno, ali ne i obavezno, pod pravim uglom), što predstavlja nefelometriju ili u pravcu upadne svetlosti, što predstavlja turbidimetriju. Primenom nefelometrije i turbidimetrije u imunologiji, razvile su se imunonefelometrija i imunoturbidimetrija, kao savremene tehnike za određivanje proteina i peptida, koje su zamenile klasične, dugotrajne, precipitacione tehnike u gelu. U ovom radu objašnjene su teorijske osnove rasipanja svetlosti, prikazane su sheme i delovi instrumenata nefelometra i turbidimetra i opisane su imunonefelometrija i imunoturbidimetrija koje su u širokoj upotrebi u savremenim kliničkim laboratorijama.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application
T1  - Imunonefelometrija i imunoturbidimetrija - teorijski principi, instrumenti i primena
VL  - 59
IS  - 6
SP  - 536
EP  - 550
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1308
ER  - 

@article{
author = "Kuntić, Vesna and Stojić-Vukanić, Zorica",
year = "2009",
abstract = "Nephelometry and turbidimetry are methods based on the phenomenon whereby light, passing through a medium with dispersed particles is attenuated in intensity by scattering. The intensity of the scattered light is measured at right angle (usually, but not necessarily) to the incident light beam (nephelometry) or at a forward angle (turbidimetry). Based on nephelometry and turbidimetry, immunonephelometric and immunoturbidimetric assays for the measurement of proteins have developed and expanded rapidly in recent years, progressively replacing the time-honored gel precipitation techniques. In this study, the theoretical background of light scattering theory, instrumental systems for nephelometry and turbidimetry, the nature and kinetics of the antibody-antigen reaction in fluid, and the application of immunonephelometric and immunoturbidimetric assays for specific proteins in the clinical laboratory are described., Nefelometrija i turbidimetrija su metode koje sa zasnivaju na rasipanju svetlosti: pri prolasku kroz disperzionu sredinu, svetlost se rasipa na česticama disperzione faze. Intenzitet rasute svetlosti može se meriti pod nekim uglom (uobičajeno, ali ne i obavezno, pod pravim uglom), što predstavlja nefelometriju ili u pravcu upadne svetlosti, što predstavlja turbidimetriju. Primenom nefelometrije i turbidimetrije u imunologiji, razvile su se imunonefelometrija i imunoturbidimetrija, kao savremene tehnike za određivanje proteina i peptida, koje su zamenile klasične, dugotrajne, precipitacione tehnike u gelu. U ovom radu objašnjene su teorijske osnove rasipanja svetlosti, prikazane su sheme i delovi instrumenata nefelometra i turbidimetra i opisane su imunonefelometrija i imunoturbidimetrija koje su u širokoj upotrebi u savremenim kliničkim laboratorijama.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application, Imunonefelometrija i imunoturbidimetrija - teorijski principi, instrumenti i primena",
volume = "59",
number = "6",
pages = "536-550",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1308"
}

Kuntić, V.,& Stojić-Vukanić, Z.. (2009). Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 59(6), 536-550.
https://hdl.handle.net/21.15107/rcub_farfar_1308

Kuntić V, Stojić-Vukanić Z. Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application. in Arhiv za farmaciju. 2009;59(6):536-550.
https://hdl.handle.net/21.15107/rcub_farfar_1308 .

Kuntić, Vesna, Stojić-Vukanić, Zorica, "Immunonephelometry and immunoturbidimetry: Theoretical principles, instruments and application" in Arhiv za farmaciju, 59, no. 6 (2009):536-550,
https://hdl.handle.net/21.15107/rcub_farfar_1308 .

TY  - JOUR
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kuntić, Vesna
AU  - Vujić, Zorica
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1318
AB  - The stability - indicating method is an analytical procedure used to detect a decrease in the amount of an active pharmaceutical ingredient present due to degradation. According to FDA guidelines, stability - indicating method is defined as a validated analytical procedure that accurately and precisely measures active ingredients free from potential interferences like degradation products, process impurities, excipient or other potential impurities. During stability studies, liquid chromatography is used routinely to separate and determine the analyses of interest. For implementing the stability - indicating method, several steps are needed. An active pharmaceutical substance is firstly subjected to the forced degradation studies followed with the investigations of its interactions with the excipient from the pharmaceutical preparation, the optimization of chromatographic conditions and the method validation.
AB  - Praćenje stabilnosti nekog leka je postupak kojim se određenom analitičkom metodom precizno mogu identifikovati i odrediti komponente nastale kao rezultat degradacije aktivne supstance kako u farmaceutskoj supstanci tako i u farmaceutskom proizvodu. Prema smernicama Agencije za hranu i lekove (eng. Food and drug administration, FDA), metoda za praćenje stabilnosti se definiše kao metoda farmaceutske analize kojom se precizno može kvantifikovati aktivna komponenta, u prisustvu degradacionih proizvoda, nečistoća koje potiču iz procesa sinteze, ekscipijenasa ili drugih potencijalnih nečistoća. U te svrhe, danas se najčešće primenjuje metoda tečne hromatografije pod visokim pritiskom (eng. High pressure liquid chromatography - HPLC, u daljem tekstu tečna hromatografija). Razvoj metode za praćenje stabilnosti podrazumeva nekoliko faza. U prvoj fazi se izvode studije forsirane degradacije sa čistom aktivnom supstancom, nakon toga se vrši ispitivanje interakcija aktivne supstance sa komponentama ekscipiensa farmaceutskog preparata, optimizacija hromatografskih uslova i validacija metode.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Stability-indicating method based on liquid chromatography for investigation of pharmaceutical substances and pharmaceutical preparations
T1  - Primena metode tečne hromatografije u ispitivanju stabilnosti farmaceutskih supstanci i farmaceutskih preparata
VL  - 59
IS  - 4
SP  - 294
EP  - 304
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1318
ER  - 

@article{
author = "Zečević, Mira and Jocić, Biljana and Kuntić, Vesna and Vujić, Zorica",
year = "2009",
abstract = "The stability - indicating method is an analytical procedure used to detect a decrease in the amount of an active pharmaceutical ingredient present due to degradation. According to FDA guidelines, stability - indicating method is defined as a validated analytical procedure that accurately and precisely measures active ingredients free from potential interferences like degradation products, process impurities, excipient or other potential impurities. During stability studies, liquid chromatography is used routinely to separate and determine the analyses of interest. For implementing the stability - indicating method, several steps are needed. An active pharmaceutical substance is firstly subjected to the forced degradation studies followed with the investigations of its interactions with the excipient from the pharmaceutical preparation, the optimization of chromatographic conditions and the method validation., Praćenje stabilnosti nekog leka je postupak kojim se određenom analitičkom metodom precizno mogu identifikovati i odrediti komponente nastale kao rezultat degradacije aktivne supstance kako u farmaceutskoj supstanci tako i u farmaceutskom proizvodu. Prema smernicama Agencije za hranu i lekove (eng. Food and drug administration, FDA), metoda za praćenje stabilnosti se definiše kao metoda farmaceutske analize kojom se precizno može kvantifikovati aktivna komponenta, u prisustvu degradacionih proizvoda, nečistoća koje potiču iz procesa sinteze, ekscipijenasa ili drugih potencijalnih nečistoća. U te svrhe, danas se najčešće primenjuje metoda tečne hromatografije pod visokim pritiskom (eng. High pressure liquid chromatography - HPLC, u daljem tekstu tečna hromatografija). Razvoj metode za praćenje stabilnosti podrazumeva nekoliko faza. U prvoj fazi se izvode studije forsirane degradacije sa čistom aktivnom supstancom, nakon toga se vrši ispitivanje interakcija aktivne supstance sa komponentama ekscipiensa farmaceutskog preparata, optimizacija hromatografskih uslova i validacija metode.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Stability-indicating method based on liquid chromatography for investigation of pharmaceutical substances and pharmaceutical preparations, Primena metode tečne hromatografije u ispitivanju stabilnosti farmaceutskih supstanci i farmaceutskih preparata",
volume = "59",
number = "4",
pages = "294-304",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1318"
}

Zečević, M., Jocić, B., Kuntić, V.,& Vujić, Z.. (2009). Stability-indicating method based on liquid chromatography for investigation of pharmaceutical substances and pharmaceutical preparations. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 59(4), 294-304.
https://hdl.handle.net/21.15107/rcub_farfar_1318

Zečević M, Jocić B, Kuntić V, Vujić Z. Stability-indicating method based on liquid chromatography for investigation of pharmaceutical substances and pharmaceutical preparations. in Arhiv za farmaciju. 2009;59(4):294-304.
https://hdl.handle.net/21.15107/rcub_farfar_1318 .

Zečević, Mira, Jocić, Biljana, Kuntić, Vesna, Vujić, Zorica, "Stability-indicating method based on liquid chromatography for investigation of pharmaceutical substances and pharmaceutical preparations" in Arhiv za farmaciju, 59, no. 4 (2009):294-304,
https://hdl.handle.net/21.15107/rcub_farfar_1318 .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Uskoković-Marković, Snežana
AU  - Kuntić, Vesna
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1266
AB  - A method for separation of three antidepressants, maprotiline, desipramine, and moclobemide, by reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated. To find optimal conditions and estimate the impact of individual parameters on the separation, a complete set of 23 interdependent relationships of the mobile phase composition, temperature, and the volume flow rate were examined. Full separation of the investigated components from a laboratory mixture was achieved on a Supelcosil LC-18 (120mmx4.6mm, 5 mu m) column, using two solvent systems, 3% ammonium ion in water/ethanol and acetonitrile, and alternating isocratic gradient-isocratic elution modes. Relevance of the proposed method for therapeutic drug monitoring is anticipated.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Analytical Letters
T1  - Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization
VL  - 42
IS  - 13
SP  - 2060
EP  - 2070
DO  - 10.1080/00032710903082747
ER  - 

@article{
author = "Vujić, Zorica and Uskoković-Marković, Snežana and Kuntić, Vesna",
year = "2009",
abstract = "A method for separation of three antidepressants, maprotiline, desipramine, and moclobemide, by reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated. To find optimal conditions and estimate the impact of individual parameters on the separation, a complete set of 23 interdependent relationships of the mobile phase composition, temperature, and the volume flow rate were examined. Full separation of the investigated components from a laboratory mixture was achieved on a Supelcosil LC-18 (120mmx4.6mm, 5 mu m) column, using two solvent systems, 3% ammonium ion in water/ethanol and acetonitrile, and alternating isocratic gradient-isocratic elution modes. Relevance of the proposed method for therapeutic drug monitoring is anticipated.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Analytical Letters",
title = "Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization",
volume = "42",
number = "13",
pages = "2060-2070",
doi = "10.1080/00032710903082747"
}

Vujić, Z., Uskoković-Marković, S.,& Kuntić, V.. (2009). Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization. in Analytical Letters
Taylor & Francis Inc, Philadelphia., 42(13), 2060-2070.
https://doi.org/10.1080/00032710903082747

Vujić Z, Uskoković-Marković S, Kuntić V. Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization. in Analytical Letters. 2009;42(13):2060-2070.
doi:10.1080/00032710903082747 .

Vujić, Zorica, Uskoković-Marković, Snežana, Kuntić, Vesna, "Simultaneous Determination of Maprotiline, Desipramine, and Moclobemide by Reversed-Phase High-Performance Liquid Chromatography and Statistical Optimization" in Analytical Letters, 42, no. 13 (2009):2060-2070,
https://doi.org/10.1080/00032710903082747 . .

TY  - JOUR
AU  - Crevar, Milkica
AU  - Ivković, Branka
AU  - Vladimirov, Sote
AU  - Kuntić, Vesna
AU  - Vujić, Zorica
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1064
AB  - Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm x 4.6 mm, 5 mu m), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 degrees C. UV detection was performed at 230 nm.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol
VL  - 55
IS  - 3
SP  - 665
EP  - 670
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1064
ER  - 

@article{
author = "Crevar, Milkica and Ivković, Branka and Vladimirov, Sote and Kuntić, Vesna and Vujić, Zorica",
year = "2008",
abstract = "Paracetamol, analgoantipyretic and anti-inflammatory drug, is available in numerous pharmaceutical formulations in common combination with caffeine and some other drug substances. p-aminophenol is paracatamol's process-related impurity that also may be present in formulations containing paracetamol. This paper presents a RP-HPLC method for simultaneous determination of caffeine, paracetamol and p-aminophenol, using chromatographic system Hewlett Packard 1100 series. In defining optimal RP-HPLC chromatographic conditions for the separation of these three compounds, experimental design was applied. Completely separation was achieved using Zorbax Extend C18 column (150 mm x 4.6 mm, 5 mu m), with mobile phase consisting of methanol-phosphate buffer pH 6 (20:80 v/v), flow rate of 1 ml/min, and column temperature of 30 degrees C. UV detection was performed at 230 nm.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol",
volume = "55",
number = "3",
pages = "665-670",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1064"
}

Crevar, M., Ivković, B., Vladimirov, S., Kuntić, V.,& Vujić, Z.. (2008). Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 55(3), 665-670.
https://hdl.handle.net/21.15107/rcub_farfar_1064

Crevar M, Ivković B, Vladimirov S, Kuntić V, Vujić Z. Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol. in Acta Chimica Slovenica. 2008;55(3):665-670.
https://hdl.handle.net/21.15107/rcub_farfar_1064 .

Crevar, Milkica, Ivković, Branka, Vladimirov, Sote, Kuntić, Vesna, Vujić, Zorica, "Statistical Optimization of Reverse Phase High Performance Liquid Chromatography for the Analysis of Caffeine Paracetamol and its Degradation Product p-aminophenol" in Acta Chimica Slovenica, 55, no. 3 (2008):665-670,
https://hdl.handle.net/21.15107/rcub_farfar_1064 .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Kuntić, Vesna
AU  - Ivković, Branka
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1059
AB  - A method of Capillary Zone Electrophoresis (CZE) for separation of three most-frequently prescribed antidepressants in the market: maprotiline, desipramine, and moclobemide was developed. The proposed method is fully validated for a ternary laboratory mixture but it is also suitable for individual determination of the investigated components in pharmaceutical dosage forms. Since the preliminary investigations did not show complete separation because of close migration time of desipramine and maprotiline, a complete set, 2(3) experimental design was applied. All the factors that affect separation as well as their mutual interactions were investigated. Voltage, temperature of capillary and the pH of phosphate buffer were independent variables or factors to be investigated in two levels. Applying response surface methodology, from experimental points the appropriate graphs were constructed and optimal chromatographic conditions for the separation were defined. The optimum conditions were: running voltage of 20kV, phosphate buffer (pH = 2.35), temperature 25 degrees C, and UV detection at 200nm. An uncoated (fused silica) capillary (50 mu m i.d.) with a total length of 50cm and a distance of 47cm between the injection and detection points was used.
PB  - Springer Wien, Wien
T2  - Monatshefte für Chemie Chemical Monthly
T1  - Statistical optimization applied to simultaneous determination of maprotiline, desipramine, and moclobemide by capillary zone electrophoresis
VL  - 139
IS  - 2
SP  - 81
EP  - 87
DO  - 10.1007/s00706-007-0686-2
ER  - 

@article{
author = "Vujić, Zorica and Kuntić, Vesna and Ivković, Branka",
year = "2008",
abstract = "A method of Capillary Zone Electrophoresis (CZE) for separation of three most-frequently prescribed antidepressants in the market: maprotiline, desipramine, and moclobemide was developed. The proposed method is fully validated for a ternary laboratory mixture but it is also suitable for individual determination of the investigated components in pharmaceutical dosage forms. Since the preliminary investigations did not show complete separation because of close migration time of desipramine and maprotiline, a complete set, 2(3) experimental design was applied. All the factors that affect separation as well as their mutual interactions were investigated. Voltage, temperature of capillary and the pH of phosphate buffer were independent variables or factors to be investigated in two levels. Applying response surface methodology, from experimental points the appropriate graphs were constructed and optimal chromatographic conditions for the separation were defined. The optimum conditions were: running voltage of 20kV, phosphate buffer (pH = 2.35), temperature 25 degrees C, and UV detection at 200nm. An uncoated (fused silica) capillary (50 mu m i.d.) with a total length of 50cm and a distance of 47cm between the injection and detection points was used.",
publisher = "Springer Wien, Wien",
journal = "Monatshefte für Chemie Chemical Monthly",
title = "Statistical optimization applied to simultaneous determination of maprotiline, desipramine, and moclobemide by capillary zone electrophoresis",
volume = "139",
number = "2",
pages = "81-87",
doi = "10.1007/s00706-007-0686-2"
}

Vujić, Z., Kuntić, V.,& Ivković, B.. (2008). Statistical optimization applied to simultaneous determination of maprotiline, desipramine, and moclobemide by capillary zone electrophoresis. in Monatshefte für Chemie Chemical Monthly
Springer Wien, Wien., 139(2), 81-87.
https://doi.org/10.1007/s00706-007-0686-2

Vujić Z, Kuntić V, Ivković B. Statistical optimization applied to simultaneous determination of maprotiline, desipramine, and moclobemide by capillary zone electrophoresis. in Monatshefte für Chemie Chemical Monthly. 2008;139(2):81-87.
doi:10.1007/s00706-007-0686-2 .

Vujić, Zorica, Kuntić, Vesna, Ivković, Branka, "Statistical optimization applied to simultaneous determination of maprotiline, desipramine, and moclobemide by capillary zone electrophoresis" in Monatshefte für Chemie Chemical Monthly, 139, no. 2 (2008):81-87,
https://doi.org/10.1007/s00706-007-0686-2 . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Topić, Aleksandra
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1154
AB  - This article reviews the fundamental and applied aspects of chemiluminescence and its applications in both routine clinical analysis and in the diverse applications in clinical research. Chemiluminiscence reactions are basic of luminometric assays, induce ultra sensitive detection limits (attomole-zeptomole), rapid assays, and a broad range of analytical applications. In the routine clinical laboratory, chemiluminescence is now commonly used for immunoassay and DNA probe assays, in the form of a chemiluminescent labels (acridinium ester, acridinium sulfonamide) and detection reactions for peroxidase and alkaline phosphatase enzyme labels (luminol and adamantyl 1,2-dioxetane-based reactions, respectively). Chemiluminescent immunoassay test kits and automated immunoassay analyzers have been developed. There are broad range of chemiluminescent immunoassay tests which are routinely used for the measurement of the biochemistry, immunology, toxicology, virology, and endocrinology analytes in the assessment of thyroid function, fertility, myocardial damage, anemia, therapeutic drug levels, and diagnosing and monitoring drug abuse, cancer and infectious diseases (e.g. hepatitis). In clinical research, the sensitivity, dynamic range and diversity of chemiluminescent assays has led to a vast range of applications, notably in protein and nucleic acid blotting, microarray-based assays, monitoring reactive oxygen species, and as detection reactions for substances separated by HPLC and capillary electrophoresis. Nowadays, in clinical research, chemiluminescent detection techniques are used to measure enzymes expressed by reporter genes, cellular luminescence, blotted proteins (Western blotting) and nucleic acids (Northern and Southern blotting).
AB  - U ovom radu, dato je teorijsko objašnjenje nastanka hemiluminiscencije i prikazane su najznačajnije hemiluminiscentne i bioluminiscentne rekcije. Na bazi hemiluminiscencije razvijena je moćna, visoko osetljiva analitička tehnika - luminometrija, sa detekcionionim limitom do atomola (10-18) i čak zeptomola (10-21), koja je našla široku primenu u imunohemijskim određivanjima i DNK analizama. U tu svrhu, ispitivana jedinjenja se vezuju za hemiluminiscentne obeleživače (akridinijum estar, akridinijum sulfonamid), ili se obeležavaju enzimima (peroksidazom ili alkalnom fosfatazom), koji pojačavaju signal hemiluminiscentnih reakcija. U rutinskoj praksi, primenjuje se veliki broj hemiluminiscentnih imuno-testova za merenje biohemijskih, imunoloških, toksikoloških, virusoloških i endokrinoloških analita u cilju ispitivanja funkcije tiroideje, fertiliteta, oštećenja miokarda, anemije, praćenja koncentracije leka, dijagnoze i praćenja zloupotrebe lekova, otkrivanja i praćenja terapije kancera, kao i infektivnih bolesti (npr. hepatitis). U kliničkim istraživanjima, zahvaljujući osetljivosti, brzini i raznolikosti izvođenja, došlo je do široke primene hemiluminiscencije, posebno u metodama ispitivanja proteina i nukleinskih kiselina pomoću blotting metoda (Western, Northern i Southern blotting), microarray matoda, praćenja reaktivnih kiseoničnih radikala, kao i u reakcijama detekcije supstanci, koje su prethodno razdvojene tečnom hromatografijom i kapilarnom elektroforezom. Danas se tehnike hemiluminiscentne detekcije koriste i za merenje aktivnosti enzima za čiju sintezu su odgovorni određeni geni (reporters genes).
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Chemiluminescence: Theory, reactions and application in the routine clinical laboratory and research
T1  - Hemiluminiscencija - teorijski princip, reakcije i primena u kliničkoj laboratorijskoj praksi i istraživanjima
VL  - 58
IS  - 4
SP  - 252
EP  - 268
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1154
ER  - 

@article{
author = "Kuntić, Vesna and Topić, Aleksandra",
year = "2008",
abstract = "This article reviews the fundamental and applied aspects of chemiluminescence and its applications in both routine clinical analysis and in the diverse applications in clinical research. Chemiluminiscence reactions are basic of luminometric assays, induce ultra sensitive detection limits (attomole-zeptomole), rapid assays, and a broad range of analytical applications. In the routine clinical laboratory, chemiluminescence is now commonly used for immunoassay and DNA probe assays, in the form of a chemiluminescent labels (acridinium ester, acridinium sulfonamide) and detection reactions for peroxidase and alkaline phosphatase enzyme labels (luminol and adamantyl 1,2-dioxetane-based reactions, respectively). Chemiluminescent immunoassay test kits and automated immunoassay analyzers have been developed. There are broad range of chemiluminescent immunoassay tests which are routinely used for the measurement of the biochemistry, immunology, toxicology, virology, and endocrinology analytes in the assessment of thyroid function, fertility, myocardial damage, anemia, therapeutic drug levels, and diagnosing and monitoring drug abuse, cancer and infectious diseases (e.g. hepatitis). In clinical research, the sensitivity, dynamic range and diversity of chemiluminescent assays has led to a vast range of applications, notably in protein and nucleic acid blotting, microarray-based assays, monitoring reactive oxygen species, and as detection reactions for substances separated by HPLC and capillary electrophoresis. Nowadays, in clinical research, chemiluminescent detection techniques are used to measure enzymes expressed by reporter genes, cellular luminescence, blotted proteins (Western blotting) and nucleic acids (Northern and Southern blotting)., U ovom radu, dato je teorijsko objašnjenje nastanka hemiluminiscencije i prikazane su najznačajnije hemiluminiscentne i bioluminiscentne rekcije. Na bazi hemiluminiscencije razvijena je moćna, visoko osetljiva analitička tehnika - luminometrija, sa detekcionionim limitom do atomola (10-18) i čak zeptomola (10-21), koja je našla široku primenu u imunohemijskim određivanjima i DNK analizama. U tu svrhu, ispitivana jedinjenja se vezuju za hemiluminiscentne obeleživače (akridinijum estar, akridinijum sulfonamid), ili se obeležavaju enzimima (peroksidazom ili alkalnom fosfatazom), koji pojačavaju signal hemiluminiscentnih reakcija. U rutinskoj praksi, primenjuje se veliki broj hemiluminiscentnih imuno-testova za merenje biohemijskih, imunoloških, toksikoloških, virusoloških i endokrinoloških analita u cilju ispitivanja funkcije tiroideje, fertiliteta, oštećenja miokarda, anemije, praćenja koncentracije leka, dijagnoze i praćenja zloupotrebe lekova, otkrivanja i praćenja terapije kancera, kao i infektivnih bolesti (npr. hepatitis). U kliničkim istraživanjima, zahvaljujući osetljivosti, brzini i raznolikosti izvođenja, došlo je do široke primene hemiluminiscencije, posebno u metodama ispitivanja proteina i nukleinskih kiselina pomoću blotting metoda (Western, Northern i Southern blotting), microarray matoda, praćenja reaktivnih kiseoničnih radikala, kao i u reakcijama detekcije supstanci, koje su prethodno razdvojene tečnom hromatografijom i kapilarnom elektroforezom. Danas se tehnike hemiluminiscentne detekcije koriste i za merenje aktivnosti enzima za čiju sintezu su odgovorni određeni geni (reporters genes).",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Chemiluminescence: Theory, reactions and application in the routine clinical laboratory and research, Hemiluminiscencija - teorijski princip, reakcije i primena u kliničkoj laboratorijskoj praksi i istraživanjima",
volume = "58",
number = "4",
pages = "252-268",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1154"
}

Kuntić, V.,& Topić, A.. (2008). Chemiluminescence: Theory, reactions and application in the routine clinical laboratory and research. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 58(4), 252-268.
https://hdl.handle.net/21.15107/rcub_farfar_1154

Kuntić V, Topić A. Chemiluminescence: Theory, reactions and application in the routine clinical laboratory and research. in Arhiv za farmaciju. 2008;58(4):252-268.
https://hdl.handle.net/21.15107/rcub_farfar_1154 .

Kuntić, Vesna, Topić, Aleksandra, "Chemiluminescence: Theory, reactions and application in the routine clinical laboratory and research" in Arhiv za farmaciju, 58, no. 4 (2008):252-268,
https://hdl.handle.net/21.15107/rcub_farfar_1154 .

TY  - JOUR
AU  - Malešev, Dušan
AU  - Kuntić, Vesna
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/998
AB  - Flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties which are overwhelmingly exerted through direct free radical scavenging. Flavonoids also exhibit antioxidant properties through chelating with transition metals, primarily Fe(II), Fe(III) and Cu(II), which participate in reactions generating free radicals. Metal-flavonoid chelates are considerably more potent free radical scavengers than the parent flavonoids and play a prominent role in protecting from oxidative stress. To unravel the origin of their potent biological action extensive physico-chemical studies were undertaken to reveal the chemical structure, chelation sites, assess the impact of the metal/ligand ratio on the structure of the complexes and the capacity of flavonoids to bind metal ions. In spite of such extensive efforts, data on the composition, structure and complex-formation properties are incomplete and sometimes even contradictory. The aim of this paper is to give a personal account on the development of the field through a retrospective evaluation of our own research which covers approximately 40 complexes of flavonoids from different flavonoids subclasses (rutin, quercetin, 3-hydroxyflavone, morin and hesperidin) with several metal ions or groups and suggest directions for future research. Special emphasis will be given to the site of the central ion, the composition of the complexes, the role of pH in complex formation, the stability of metal-flavonoid complexes and their potential application for analytical purposes. .
AB  - Flavonoidi predstavljaju veliku grupu polifenolnih jedinjenja biljnog porekla, koji imaju antioksidantna svojstva zbog direktnog "hvatanja" slobodnih radikala. Antioksidantno dejstvo flavonoidi takođe ostvaruju stvaranjem helata sa jonima prelaznih metala, prvenstveno sa jonima Fe(II), Fe(III) i Cu(II), koji sami učestvuju u reakciji stvaranja slobodnih radikala. Helati metal-flavonoid su mnogo efikasniji "hvatači" slobodnih radikala od matičnih flavonoida i mogu zaštititi potencijalne mete u organizmu od oksidativnog stresa. Da bi se razjasnio mehanizam biološkog delovanja kompleksa metal-flavonoid, ispitivane su fizičko-hemijske karakteristike kompleksa: mesto vezivanja jona metala, zavisnost strukture kompleksa od odnosa metal/ligand, afinitet flavonoida za vezivanje metalnog jona, itd. Međutim, podaci o sastavu, strukturi i osobinama kompleksa su nekompletni i ponekad kontradiktorni. Stoga je namera ovog rada da kroz retrospektivu naših rezultata damo lični doprinos u ispitivanju metal-flavonoid kompleksa. U prikazanom revijalnom radu sakupljeni su svi naši rezultati o približno 40 kompleksa obrazovanih između pet flavonoida (rutin, kvercetin, 3-hidroksiflavon, morin i hesperidin) i većeg broja metalnih jona ili metalnih grupa. Istaknuti su podaci o mestu vezivanja centralnog jona u kompleksu, stehiometrijskom odnosu metal-ligand, uticaju pH na formiranje kompleksa i konstantama stabilnosti kompleksa. Takođe, u ovom radu prikazali smo i primenu metal-flavonoid kompleksa u analitičke svrhe. .
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions
T1  - Ispitivanje metal-flavonoid helata i određivanje flavonoida preko kompleksirajuće reakcije metal-flavonoid
VL  - 72
IS  - 10
SP  - 921
EP  - 939
DO  - 10.2298/JSC0710921M
ER  - 

@article{
author = "Malešev, Dušan and Kuntić, Vesna",
year = "2007",
abstract = "Flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties which are overwhelmingly exerted through direct free radical scavenging. Flavonoids also exhibit antioxidant properties through chelating with transition metals, primarily Fe(II), Fe(III) and Cu(II), which participate in reactions generating free radicals. Metal-flavonoid chelates are considerably more potent free radical scavengers than the parent flavonoids and play a prominent role in protecting from oxidative stress. To unravel the origin of their potent biological action extensive physico-chemical studies were undertaken to reveal the chemical structure, chelation sites, assess the impact of the metal/ligand ratio on the structure of the complexes and the capacity of flavonoids to bind metal ions. In spite of such extensive efforts, data on the composition, structure and complex-formation properties are incomplete and sometimes even contradictory. The aim of this paper is to give a personal account on the development of the field through a retrospective evaluation of our own research which covers approximately 40 complexes of flavonoids from different flavonoids subclasses (rutin, quercetin, 3-hydroxyflavone, morin and hesperidin) with several metal ions or groups and suggest directions for future research. Special emphasis will be given to the site of the central ion, the composition of the complexes, the role of pH in complex formation, the stability of metal-flavonoid complexes and their potential application for analytical purposes. ., Flavonoidi predstavljaju veliku grupu polifenolnih jedinjenja biljnog porekla, koji imaju antioksidantna svojstva zbog direktnog "hvatanja" slobodnih radikala. Antioksidantno dejstvo flavonoidi takođe ostvaruju stvaranjem helata sa jonima prelaznih metala, prvenstveno sa jonima Fe(II), Fe(III) i Cu(II), koji sami učestvuju u reakciji stvaranja slobodnih radikala. Helati metal-flavonoid su mnogo efikasniji "hvatači" slobodnih radikala od matičnih flavonoida i mogu zaštititi potencijalne mete u organizmu od oksidativnog stresa. Da bi se razjasnio mehanizam biološkog delovanja kompleksa metal-flavonoid, ispitivane su fizičko-hemijske karakteristike kompleksa: mesto vezivanja jona metala, zavisnost strukture kompleksa od odnosa metal/ligand, afinitet flavonoida za vezivanje metalnog jona, itd. Međutim, podaci o sastavu, strukturi i osobinama kompleksa su nekompletni i ponekad kontradiktorni. Stoga je namera ovog rada da kroz retrospektivu naših rezultata damo lični doprinos u ispitivanju metal-flavonoid kompleksa. U prikazanom revijalnom radu sakupljeni su svi naši rezultati o približno 40 kompleksa obrazovanih između pet flavonoida (rutin, kvercetin, 3-hidroksiflavon, morin i hesperidin) i većeg broja metalnih jona ili metalnih grupa. Istaknuti su podaci o mestu vezivanja centralnog jona u kompleksu, stehiometrijskom odnosu metal-ligand, uticaju pH na formiranje kompleksa i konstantama stabilnosti kompleksa. Takođe, u ovom radu prikazali smo i primenu metal-flavonoid kompleksa u analitičke svrhe. .",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions, Ispitivanje metal-flavonoid helata i određivanje flavonoida preko kompleksirajuće reakcije metal-flavonoid",
volume = "72",
number = "10",
pages = "921-939",
doi = "10.2298/JSC0710921M"
}

Malešev, D.,& Kuntić, V.. (2007). Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 72(10), 921-939.
https://doi.org/10.2298/JSC0710921M

Malešev D, Kuntić V. Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions. in Journal of the Serbian Chemical Society. 2007;72(10):921-939.
doi:10.2298/JSC0710921M .

Malešev, Dušan, Kuntić, Vesna, "Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions" in Journal of the Serbian Chemical Society, 72, no. 10 (2007):921-939,
https://doi.org/10.2298/JSC0710921M . .

TY  - JOUR
AU  - Ilić, Katarina
AU  - Kuntić, Vesna
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1015
AB  - Biomarkeri imaju široku primenu u modernoj medicini i biologiji. Oni su promenili način dijagnostikovanja i klasifikacije bolesti, praćenja efekata terapije, određivanja toksičnosti, a imaju i sve veću primenu u razvoju novih lekova. Karakteristike biomarkera određuju i njihovu primenu. Biomarkeri koji imaju malu moć predviđanja korisnosti tretmana koriste se u ranim fazama razvoja lekova za preliminarni skrining. Biomarkeri velike prediktivne moći mogu biti upotrebljeni kao surogatni parametri u proceni efikasnosti i bezbednosti tretmana. Surogatni parametri validirani u kliničkim istraživanjima i prihvaćeni od strane regulatornih agencija mogu biti korišćeni i za registraciju lekova, naročito za ubrzanu registraciju antiinfektivnih lekova i hemoterapeutika. Međutim, za registraciju antikancerskih lekova regulatorne agencije insistiraju na neophodnosti prospektivnih postmarketinških studija da bi se dokazalo postojanje korelacije surogatnih parametara sa kliničkom koristi od primenjenog tretmana. .
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Significance of biomarkers and surrogate endpoints in preclinical and clinical trials
T1  - Uloga biomarkera i surogatnih parametara u pretkliničkim i kliničkim ispitivanjima lekova
VL  - 64
IS  - 8
SP  - 561
EP  - 567
DO  - 10.2298/VSP0708561I
ER  - 

@article{
author = "Ilić, Katarina and Kuntić, Vesna",
year = "2007",
abstract = "Biomarkeri imaju široku primenu u modernoj medicini i biologiji. Oni su promenili način dijagnostikovanja i klasifikacije bolesti, praćenja efekata terapije, određivanja toksičnosti, a imaju i sve veću primenu u razvoju novih lekova. Karakteristike biomarkera određuju i njihovu primenu. Biomarkeri koji imaju malu moć predviđanja korisnosti tretmana koriste se u ranim fazama razvoja lekova za preliminarni skrining. Biomarkeri velike prediktivne moći mogu biti upotrebljeni kao surogatni parametri u proceni efikasnosti i bezbednosti tretmana. Surogatni parametri validirani u kliničkim istraživanjima i prihvaćeni od strane regulatornih agencija mogu biti korišćeni i za registraciju lekova, naročito za ubrzanu registraciju antiinfektivnih lekova i hemoterapeutika. Međutim, za registraciju antikancerskih lekova regulatorne agencije insistiraju na neophodnosti prospektivnih postmarketinških studija da bi se dokazalo postojanje korelacije surogatnih parametara sa kliničkom koristi od primenjenog tretmana. .",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Significance of biomarkers and surrogate endpoints in preclinical and clinical trials, Uloga biomarkera i surogatnih parametara u pretkliničkim i kliničkim ispitivanjima lekova",
volume = "64",
number = "8",
pages = "561-567",
doi = "10.2298/VSP0708561I"
}

Ilić, K.,& Kuntić, V.. (2007). Significance of biomarkers and surrogate endpoints in preclinical and clinical trials. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 64(8), 561-567.
https://doi.org/10.2298/VSP0708561I

Ilić K, Kuntić V. Significance of biomarkers and surrogate endpoints in preclinical and clinical trials. in Vojnosanitetski pregled. 2007;64(8):561-567.
doi:10.2298/VSP0708561I .

Ilić, Katarina, Kuntić, Vesna, "Significance of biomarkers and surrogate endpoints in preclinical and clinical trials" in Vojnosanitetski pregled, 64, no. 8 (2007):561-567,
https://doi.org/10.2298/VSP0708561I . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Ivković, Branka
AU  - Vujić, Zorica
AU  - Ilić, Katarina
AU  - Micić, Svetlana
AU  - Vukojević, Vladana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/944
AB  - A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Isocratic RP-HPLC method for rutin determination in solid oral dosage forms
VL  - 43
IS  - 2
SP  - 718
EP  - 721
DO  - 10.1016/j.jpba.2006.07.019
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Ivković, Branka and Vujić, Zorica and Ilić, Katarina and Micić, Svetlana and Vukojević, Vladana",
year = "2007",
abstract = "A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms",
volume = "43",
number = "2",
pages = "718-721",
doi = "10.1016/j.jpba.2006.07.019"
}

Kuntić, V., Pejić, N., Ivković, B., Vujić, Z., Ilić, K., Micić, S.,& Vukojević, V.. (2007). Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 43(2), 718-721.
https://doi.org/10.1016/j.jpba.2006.07.019

Kuntić V, Pejić N, Ivković B, Vujić Z, Ilić K, Micić S, Vukojević V. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis. 2007;43(2):718-721.
doi:10.1016/j.jpba.2006.07.019 .

Kuntić, Vesna, Pejić, Nataša, Ivković, Branka, Vujić, Zorica, Ilić, Katarina, Micić, Svetlana, Vukojević, Vladana, "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms" in Journal of Pharmaceutical and Biomedical Analysis, 43, no. 2 (2007):718-721,
https://doi.org/10.1016/j.jpba.2006.07.019 . .

TY  - CONF
AU  - Malešev, Dušan
AU  - Kuntić, Vesna
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/840
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Determination of flavonoids via metal-flavonoid complexing reactions
T1  - Ispitivanje helata metal-flavonoid i određivanje flavonoida pomoću kompleksirajuće reakcije metal-flavonoid
VL  - 56
IS  - 5
SP  - 878
EP  - 879
UR  - https://hdl.handle.net/21.15107/rcub_farfar_840
ER  - 

@conference{
author = "Malešev, Dušan and Kuntić, Vesna",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Determination of flavonoids via metal-flavonoid complexing reactions, Ispitivanje helata metal-flavonoid i određivanje flavonoida pomoću kompleksirajuće reakcije metal-flavonoid",
volume = "56",
number = "5",
pages = "878-879",
url = "https://hdl.handle.net/21.15107/rcub_farfar_840"
}

Malešev, D.,& Kuntić, V.. (2006). Determination of flavonoids via metal-flavonoid complexing reactions. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 878-879.
https://hdl.handle.net/21.15107/rcub_farfar_840

Malešev D, Kuntić V. Determination of flavonoids via metal-flavonoid complexing reactions. in Arhiv za farmaciju. 2006;56(5):878-879.
https://hdl.handle.net/21.15107/rcub_farfar_840 .

Malešev, Dušan, Kuntić, Vesna, "Determination of flavonoids via metal-flavonoid complexing reactions" in Arhiv za farmaciju, 56, no. 5 (2006):878-879,
https://hdl.handle.net/21.15107/rcub_farfar_840 .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Stanojević, Maja
AU  - Holclajtner-Antunović, Ivanka
AU  - Uskoković-Marković, Snežana
AU  - Mioč, Ubavka B.
AU  - Todorović, Marija R.
AU  - Jovanović, Tanja
AU  - Vukojević, Vladana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/837
AB  - Compounds of phosphotungstic acid (WPA) containing the amino acids alanine (WPA-Ala) or glycine (WPA-Gly) as counter cations were synthesized and characterized by elemental analysis, thermal analysis, and IR spectroscopy. Cellular toxicity was assessed by the trypan blue exclusion method, and the antiviral activity of WPA and the modified WPA compounds was tested against herpes simplex viruses (HSV) type 1 and type 2. Biological assays indicate that the newly synthesized compounds exhibit no evident cytotoxic effects on Vero cells and negligible antiviral activity against HSV-1 and HSV-2.
PB  - Springer Wien, Wien
T2  - Monatshefte für Chemie Chemical Monthly
T1  - Synthesis, characterization, and biological activity of amino acid derivatives of the heteropolytungstophosphoric acid
VL  - 137
IS  - 6
SP  - 803
EP  - 810
DO  - 10.1007/s00706-006-0467-3
ER  - 

@article{
author = "Kuntić, Vesna and Stanojević, Maja and Holclajtner-Antunović, Ivanka and Uskoković-Marković, Snežana and Mioč, Ubavka B. and Todorović, Marija R. and Jovanović, Tanja and Vukojević, Vladana",
year = "2006",
abstract = "Compounds of phosphotungstic acid (WPA) containing the amino acids alanine (WPA-Ala) or glycine (WPA-Gly) as counter cations were synthesized and characterized by elemental analysis, thermal analysis, and IR spectroscopy. Cellular toxicity was assessed by the trypan blue exclusion method, and the antiviral activity of WPA and the modified WPA compounds was tested against herpes simplex viruses (HSV) type 1 and type 2. Biological assays indicate that the newly synthesized compounds exhibit no evident cytotoxic effects on Vero cells and negligible antiviral activity against HSV-1 and HSV-2.",
publisher = "Springer Wien, Wien",
journal = "Monatshefte für Chemie Chemical Monthly",
title = "Synthesis, characterization, and biological activity of amino acid derivatives of the heteropolytungstophosphoric acid",
volume = "137",
number = "6",
pages = "803-810",
doi = "10.1007/s00706-006-0467-3"
}

Kuntić, V., Stanojević, M., Holclajtner-Antunović, I., Uskoković-Marković, S., Mioč, U. B., Todorović, M. R., Jovanović, T.,& Vukojević, V.. (2006). Synthesis, characterization, and biological activity of amino acid derivatives of the heteropolytungstophosphoric acid. in Monatshefte für Chemie Chemical Monthly
Springer Wien, Wien., 137(6), 803-810.
https://doi.org/10.1007/s00706-006-0467-3

Kuntić V, Stanojević M, Holclajtner-Antunović I, Uskoković-Marković S, Mioč UB, Todorović MR, Jovanović T, Vukojević V. Synthesis, characterization, and biological activity of amino acid derivatives of the heteropolytungstophosphoric acid. in Monatshefte für Chemie Chemical Monthly. 2006;137(6):803-810.
doi:10.1007/s00706-006-0467-3 .

Kuntić, Vesna, Stanojević, Maja, Holclajtner-Antunović, Ivanka, Uskoković-Marković, Snežana, Mioč, Ubavka B., Todorović, Marija R., Jovanović, Tanja, Vukojević, Vladana, "Synthesis, characterization, and biological activity of amino acid derivatives of the heteropolytungstophosphoric acid" in Monatshefte für Chemie Chemical Monthly, 137, no. 6 (2006):803-810,
https://doi.org/10.1007/s00706-006-0467-3 . .