TY  - JOUR
AU  - Smajić, Miralem
AU  - Vujić, Zorica
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3164
AB  - Central composite experimental design was used for fast, simple, and accurate high-performance liquid chromatography (HPLC) determination of hydrochlorothiazide, amlodipine and valsartan in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow establishing the multiple interacting parameters. On the basis of preliminary experiments and physicochemical characteristic of analyzed substances, three independent variables (methanol content, pH of the mobile phase, and column temperature) were selected as input, while as dependent variables, six responses (retention time of hydrochlorothiazide, retention time of amlodipine, retention time of valsartan, asymmetry of hydrochlorothiazide peak, asymmetry of amlodipine peak, and asymmetry of valsartan peak) were chosen. Face centered central composite design enables an estimation of factors which have the most importance. After optimizing experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 40: 20: 40 (v/v/v), pH adjusted to 3.5 with acetic acid, flow rate of 1 mL/min and column temperature of 40 C-deg. The method was successfully applied to the simultaneous separations of these active drug compounds in their commercial dosage forms.
PB  - Ios Press, Amsterdam
T2  - Main Group Chemistry
T1  - Application of central composite design and structural analysis to the optimization of HPLC method for simultaneous analysis of hydrochlorothiazide, amlodipine besylate and valsartan
VL  - 17
IS  - 2
SP  - 165
EP  - 180
DO  - 10.3233/MGC-180259
ER  - 

@article{
author = "Smajić, Miralem and Vujić, Zorica",
year = "2018",
abstract = "Central composite experimental design was used for fast, simple, and accurate high-performance liquid chromatography (HPLC) determination of hydrochlorothiazide, amlodipine and valsartan in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow establishing the multiple interacting parameters. On the basis of preliminary experiments and physicochemical characteristic of analyzed substances, three independent variables (methanol content, pH of the mobile phase, and column temperature) were selected as input, while as dependent variables, six responses (retention time of hydrochlorothiazide, retention time of amlodipine, retention time of valsartan, asymmetry of hydrochlorothiazide peak, asymmetry of amlodipine peak, and asymmetry of valsartan peak) were chosen. Face centered central composite design enables an estimation of factors which have the most importance. After optimizing experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 40: 20: 40 (v/v/v), pH adjusted to 3.5 with acetic acid, flow rate of 1 mL/min and column temperature of 40 C-deg. The method was successfully applied to the simultaneous separations of these active drug compounds in their commercial dosage forms.",
publisher = "Ios Press, Amsterdam",
journal = "Main Group Chemistry",
title = "Application of central composite design and structural analysis to the optimization of HPLC method for simultaneous analysis of hydrochlorothiazide, amlodipine besylate and valsartan",
volume = "17",
number = "2",
pages = "165-180",
doi = "10.3233/MGC-180259"
}

Smajić, M.,& Vujić, Z.. (2018). Application of central composite design and structural analysis to the optimization of HPLC method for simultaneous analysis of hydrochlorothiazide, amlodipine besylate and valsartan. in Main Group Chemistry
Ios Press, Amsterdam., 17(2), 165-180.
https://doi.org/10.3233/MGC-180259

Smajić M, Vujić Z. Application of central composite design and structural analysis to the optimization of HPLC method for simultaneous analysis of hydrochlorothiazide, amlodipine besylate and valsartan. in Main Group Chemistry. 2018;17(2):165-180.
doi:10.3233/MGC-180259 .

Smajić, Miralem, Vujić, Zorica, "Application of central composite design and structural analysis to the optimization of HPLC method for simultaneous analysis of hydrochlorothiazide, amlodipine besylate and valsartan" in Main Group Chemistry, 17, no. 2 (2018):165-180,
https://doi.org/10.3233/MGC-180259 . .

TY  - JOUR
AU  - Smajić, Miralem
AU  - Nikolić, Katarina
AU  - Vujić, Zorica
AU  - Ahmetović, Lejla
AU  - Kuntić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2574
AB  - A 3D-QSAR model using the GRIND/ALMOND descriptors has been performed on a set of 49 angiotensin receptor blockers, also known as angiotensin II receptor antagonists, a family of agents that bind to and inhibit the angiotensin II type 1 receptor. The most commonly used chemical probes: DRY (hydrophobic interaction), O (carbonyl oxygen sp(2), hydrogen bond donor), N1 (NH neutral, hydrogen bond acceptor) and TIP (shape descriptor molecular forms) were derived from the GRID molecular interaction fields. A statistical approach was undertaken using the method of partial least squares within the Pentacle program. The results show satisfactory accuracy of the prediction model (RMSEE = 0.239, R-2 = 0.94, Q(2) = 0.85). The V597 (DRY-TIP) and V763 (O-TIP) represent the most significant variables that correlate positively with the activity of the ARBs. Thirty novel structures of ARBs were designed, according to the developed 3D-QSAR model and pharmacophore, what might set the basis for development of new antihypertensive drugs.
PB  - Springer Birkhauser, New York
T2  - Medicinal Chemistry Research
T1  - 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists
VL  - 25
IS  - 1
SP  - 51
EP  - 61
DO  - 10.1007/s00044-015-1470-1
ER  - 

@article{
author = "Smajić, Miralem and Nikolić, Katarina and Vujić, Zorica and Ahmetović, Lejla and Kuntić, Vesna",
year = "2016",
abstract = "A 3D-QSAR model using the GRIND/ALMOND descriptors has been performed on a set of 49 angiotensin receptor blockers, also known as angiotensin II receptor antagonists, a family of agents that bind to and inhibit the angiotensin II type 1 receptor. The most commonly used chemical probes: DRY (hydrophobic interaction), O (carbonyl oxygen sp(2), hydrogen bond donor), N1 (NH neutral, hydrogen bond acceptor) and TIP (shape descriptor molecular forms) were derived from the GRID molecular interaction fields. A statistical approach was undertaken using the method of partial least squares within the Pentacle program. The results show satisfactory accuracy of the prediction model (RMSEE = 0.239, R-2 = 0.94, Q(2) = 0.85). The V597 (DRY-TIP) and V763 (O-TIP) represent the most significant variables that correlate positively with the activity of the ARBs. Thirty novel structures of ARBs were designed, according to the developed 3D-QSAR model and pharmacophore, what might set the basis for development of new antihypertensive drugs.",
publisher = "Springer Birkhauser, New York",
journal = "Medicinal Chemistry Research",
title = "3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists",
volume = "25",
number = "1",
pages = "51-61",
doi = "10.1007/s00044-015-1470-1"
}

Smajić, M., Nikolić, K., Vujić, Z., Ahmetović, L.,& Kuntić, V.. (2016). 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists. in Medicinal Chemistry Research
Springer Birkhauser, New York., 25(1), 51-61.
https://doi.org/10.1007/s00044-015-1470-1

Smajić M, Nikolić K, Vujić Z, Ahmetović L, Kuntić V. 3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists. in Medicinal Chemistry Research. 2016;25(1):51-61.
doi:10.1007/s00044-015-1470-1 .

Smajić, Miralem, Nikolić, Katarina, Vujić, Zorica, Ahmetović, Lejla, Kuntić, Vesna, "3D-QSAR studies and pharmacophore identification of AT(1) receptor antagonists" in Medicinal Chemistry Research, 25, no. 1 (2016):51-61,
https://doi.org/10.1007/s00044-015-1470-1 . .

TY  - JOUR
AU  - Smajić, Miralem
AU  - Vujić, Zorica
AU  - Mulavdić, Nedzad
AU  - Brborić, Jasmina
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1930
AB  - An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol
VL  - 76
IS  - 7-8
SP  - 419
EP  - 425
DO  - 10.1007/s10337-013-2388-8
ER  - 

@article{
author = "Smajić, Miralem and Vujić, Zorica and Mulavdić, Nedzad and Brborić, Jasmina",
year = "2013",
abstract = "An experimental design method was used for fast, simple, and accurate high-performance-pressure liquid chromatograpy (HPLC) determination of losartan and hydrochlorothiazide in combined dosage forms. This method avoids the disadvantages of the traditional analytical approach, which is time-consuming, involves a large number of runs, and does not allow the determination of multiple interacting parameters. On the basis of preliminary experiments, three independent variables (methanol content, pH value of the mobile phase, and flow rate) were selected as input, and as dependent variables, five responses (retention time of hydrochlorothiazide, retention time of losartan, asymmetry of hydrochlorothiazide peak, asymmetry of losartan peak, and resolution) were chosen. A full 2(3) factorial design was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influencing parameters in the previously selected experimental domain. After optimizing the experimental conditions, a separation was conducted on a Zorbax C-8 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-acetonitrile-acetate buffer 45:20:35 v/v/v, pH 4.8 with flow rate of 0.82 mL min(-1) and column temperature of 25 A degrees C. The developed method was successfully applied to simultaneous separation of these active drug compounds in their commercial pharmaceutical dosage forms.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol",
volume = "76",
number = "7-8",
pages = "419-425",
doi = "10.1007/s10337-013-2388-8"
}

Smajić, M., Vujić, Z., Mulavdić, N.,& Brborić, J.. (2013). An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia
Springer Heidelberg, Heidelberg., 76(7-8), 419-425.
https://doi.org/10.1007/s10337-013-2388-8

Smajić M, Vujić Z, Mulavdić N, Brborić J. An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol. in Chromatographia. 2013;76(7-8):419-425.
doi:10.1007/s10337-013-2388-8 .

Smajić, Miralem, Vujić, Zorica, Mulavdić, Nedzad, Brborić, Jasmina, "An Improved HPLC Method for Simultaneous Analysis of Losartan Potassium and Hydrochlorothiazide with the Aid of a Chemometric Protocol" in Chromatographia, 76, no. 7-8 (2013):419-425,
https://doi.org/10.1007/s10337-013-2388-8 . .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Mulavdić, Nedzad
AU  - Smajić, Miralem
AU  - Brborić, Jasmina
AU  - Stanković, Predrag
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1703
AB  - Experimental design method was used for HPLC determination of irbesartan and hydrochlorothiazide in combined dosage forms. The traditional approach for optimization of experiments is time-consuming, involves a large number of runs and does not allow establishing the multiple interacting parameters. The main advantages of the experimental design method include the simultaneous screening of a larger number of factors affecting response and the estimation of possible interactions. On the basis of preliminary experiments, three factors-independent variables were selected as inputs (methanol content, pH of the mobile phase and temperature) and as dependent variables, five responses (resolution, symmetry of irbesartan peak, symmetry of hydrochlorothiazide peak, retention factor of irbesartan and retention factor of hydrochlorothiazide) were chosen. A full 2(3) factorial design, where factors were examined at two different levels ("low" and "high") was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influent parameters in the previously selected experimental domain. The novelty of our method lies in the optimization step accomplished by Derringer's desirability function. After optimizing the experimental conditions a separation was conducted on a Supelcosil C-18 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-tetrahydrofuran-acetate buffer 47:10:43 v/v/v, pH 6.5 and a column temperature of 25 degrees C. The developed method was successfully applied to the simultaneous separation of these drug-active compounds in their commercial pharmaceutical dosage forms.
PB  - MDPI, Basel
T2  - Molecules
T1  - Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol
VL  - 17
IS  - 3
SP  - 3461
EP  - 3474
DO  - 10.3390/molecules17033461
ER  - 

@article{
author = "Vujić, Zorica and Mulavdić, Nedzad and Smajić, Miralem and Brborić, Jasmina and Stanković, Predrag",
year = "2012",
abstract = "Experimental design method was used for HPLC determination of irbesartan and hydrochlorothiazide in combined dosage forms. The traditional approach for optimization of experiments is time-consuming, involves a large number of runs and does not allow establishing the multiple interacting parameters. The main advantages of the experimental design method include the simultaneous screening of a larger number of factors affecting response and the estimation of possible interactions. On the basis of preliminary experiments, three factors-independent variables were selected as inputs (methanol content, pH of the mobile phase and temperature) and as dependent variables, five responses (resolution, symmetry of irbesartan peak, symmetry of hydrochlorothiazide peak, retention factor of irbesartan and retention factor of hydrochlorothiazide) were chosen. A full 2(3) factorial design, where factors were examined at two different levels ("low" and "high") was used to determine which factors had an effect on the studied response. Afterwards, experimental design was used to optimize these influent parameters in the previously selected experimental domain. The novelty of our method lies in the optimization step accomplished by Derringer's desirability function. After optimizing the experimental conditions a separation was conducted on a Supelcosil C-18 (150 mm x 4.6 mm, 5 mu m particle size) column with a mobile phase consisting of methanol-tetrahydrofuran-acetate buffer 47:10:43 v/v/v, pH 6.5 and a column temperature of 25 degrees C. The developed method was successfully applied to the simultaneous separation of these drug-active compounds in their commercial pharmaceutical dosage forms.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol",
volume = "17",
number = "3",
pages = "3461-3474",
doi = "10.3390/molecules17033461"
}

Vujić, Z., Mulavdić, N., Smajić, M., Brborić, J.,& Stanković, P.. (2012). Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol. in Molecules
MDPI, Basel., 17(3), 3461-3474.
https://doi.org/10.3390/molecules17033461

Vujić Z, Mulavdić N, Smajić M, Brborić J, Stanković P. Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol. in Molecules. 2012;17(3):3461-3474.
doi:10.3390/molecules17033461 .

Vujić, Zorica, Mulavdić, Nedzad, Smajić, Miralem, Brborić, Jasmina, Stanković, Predrag, "Simultaneous Analysis of Irbesartan and Hydrochlorothiazide: An Improved HPLC Method with the Aid of a Chemometric Protocol" in Molecules, 17, no. 3 (2012):3461-3474,
https://doi.org/10.3390/molecules17033461 . .