TY  - JOUR
AU  - Renieri, Elisavet A.
AU  - Goumenou, Marina
AU  - Kardonsky, Dmitry A.
AU  - Veselov, Valery V.
AU  - Alegakis, Athanasios K.
AU  - Buha, Aleksandra
AU  - Tzatzarakis, Manolis N.
AU  - Nosyrev, Alexander E.
AU  - Rakitskii, Valerii N.
AU  - Kentouri, Maroudio
AU  - Tsatsakis, Aristidis
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3371
AB  - Health benefits of fish consumption could be counterbalanced by the intake of contaminants after long term fish consumption, burdened even in trace levels. The presence of the indicator PCBs (NDL-PCBs and PCB 118) in farmed and wild seabream and seabass was evaluated. For the determination of PCB, a GC-MS method was developed and evaluated. The association of PCB accumulation in fish with seasonality, locality, production mode and species was also investigated. A new approach for the risk characterisation after exposure to NDL-PCB through fish consumption in Greece was developed, based on the real exposure and the permitted maximum levels of both aggregated dietary exposure and exposure through fish consumption. PCB levels determined in fish were below established permitted limits (6.24 ng/g 95th percentile), while PCB levels and congener distribution varied significantly between farmed and wild fish (p = 0.001). Seasonality was highlighted as an important factor affecting NDL-PCBs accumulation, with high levels coinciding with the reproduction period of each species. Differences were also depicted for sampling sites, with PCB 118 presenting significantly higher values in open seas while NDL-PCB congeners in closed seas. Risk assessment of NDL-PCB intake through fish consumption corrected for the aggregated exposure revealed no risk for the consumers.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Indicator PCBs in farmed and wild fish in Greece - Risk assessment for the Greek population
VL  - 127
SP  - 260
EP  - 269
DO  - 10.1016/j.fct.2019.03.027
ER  - 

@article{
author = "Renieri, Elisavet A. and Goumenou, Marina and Kardonsky, Dmitry A. and Veselov, Valery V. and Alegakis, Athanasios K. and Buha, Aleksandra and Tzatzarakis, Manolis N. and Nosyrev, Alexander E. and Rakitskii, Valerii N. and Kentouri, Maroudio and Tsatsakis, Aristidis",
year = "2019",
abstract = "Health benefits of fish consumption could be counterbalanced by the intake of contaminants after long term fish consumption, burdened even in trace levels. The presence of the indicator PCBs (NDL-PCBs and PCB 118) in farmed and wild seabream and seabass was evaluated. For the determination of PCB, a GC-MS method was developed and evaluated. The association of PCB accumulation in fish with seasonality, locality, production mode and species was also investigated. A new approach for the risk characterisation after exposure to NDL-PCB through fish consumption in Greece was developed, based on the real exposure and the permitted maximum levels of both aggregated dietary exposure and exposure through fish consumption. PCB levels determined in fish were below established permitted limits (6.24 ng/g 95th percentile), while PCB levels and congener distribution varied significantly between farmed and wild fish (p = 0.001). Seasonality was highlighted as an important factor affecting NDL-PCBs accumulation, with high levels coinciding with the reproduction period of each species. Differences were also depicted for sampling sites, with PCB 118 presenting significantly higher values in open seas while NDL-PCB congeners in closed seas. Risk assessment of NDL-PCB intake through fish consumption corrected for the aggregated exposure revealed no risk for the consumers.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Indicator PCBs in farmed and wild fish in Greece - Risk assessment for the Greek population",
volume = "127",
pages = "260-269",
doi = "10.1016/j.fct.2019.03.027"
}

Renieri, E. A., Goumenou, M., Kardonsky, D. A., Veselov, V. V., Alegakis, A. K., Buha, A., Tzatzarakis, M. N., Nosyrev, A. E., Rakitskii, V. N., Kentouri, M.,& Tsatsakis, A.. (2019). Indicator PCBs in farmed and wild fish in Greece - Risk assessment for the Greek population. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 127, 260-269.
https://doi.org/10.1016/j.fct.2019.03.027

Renieri EA, Goumenou M, Kardonsky DA, Veselov VV, Alegakis AK, Buha A, Tzatzarakis MN, Nosyrev AE, Rakitskii VN, Kentouri M, Tsatsakis A. Indicator PCBs in farmed and wild fish in Greece - Risk assessment for the Greek population. in Food and Chemical Toxicology. 2019;127:260-269.
doi:10.1016/j.fct.2019.03.027 .

Renieri, Elisavet A., Goumenou, Marina, Kardonsky, Dmitry A., Veselov, Valery V., Alegakis, Athanasios K., Buha, Aleksandra, Tzatzarakis, Manolis N., Nosyrev, Alexander E., Rakitskii, Valerii N., Kentouri, Maroudio, Tsatsakis, Aristidis, "Indicator PCBs in farmed and wild fish in Greece - Risk assessment for the Greek population" in Food and Chemical Toxicology, 127 (2019):260-269,
https://doi.org/10.1016/j.fct.2019.03.027 . .

TY  - JOUR
AU  - Buha, Aleksandra
AU  - Matović, Vesna
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
AU  - Ćurčić, Marijana
AU  - Renieri, Elisavet A.
AU  - Tsatsakis, Aristidis
AU  - Schweitzer, Amie
AU  - Wallace, David
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3035
AB  - Humans are exposed to a significant number of chemicals that are suspected to produce disturbances in hormone homeostasis. Hence, in recent decades, there has been a growing interest in endocrine disruptive chemicals. One of the alleged thyroid disrupting substances is cadmium (Cd), a ubiquitous toxic metal shown to act as a thyroid disruptor and carcinogen in both animals and humans. Multiple PubMed searches with core keywords were performed to identify and evaluate appropriate studies which revealed literature suggesting evidence for the link between exposure to Cd and histological and metabolic changes in the thyroid gland. Furthermore, Cd influence on thyroid homeostasis at the peripheral level has also been hypothesized. Both in vivo and in vitro studies revealed that a Cd exposure at environmentally relevant concentrations results in biphasic Cd dose-thyroid response relationships. Development of thyroid tumors following exposure to Cd has been studied mainly using in vitro methodologies. In the thyroid, Cd has been shown to activate or stimulate the activity of various factors, leading to increased cell proliferation and a reduction in normal apoptotic activity. Evidence establishing the association between Cd and thyroid disruption remains ambiguous, with further studies needed to elucidate the issue and improve our understanding of Cd-mediated effects on the thyroid gland.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Overview of Cadmium Thyroid Disrupting Effects and Mechanisms
VL  - 19
IS  - 5
DO  - 10.3390/ijms19051501
ER  - 

@article{
author = "Buha, Aleksandra and Matović, Vesna and Antonijević, Biljana and Bulat, Zorica and Ćurčić, Marijana and Renieri, Elisavet A. and Tsatsakis, Aristidis and Schweitzer, Amie and Wallace, David",
year = "2018",
abstract = "Humans are exposed to a significant number of chemicals that are suspected to produce disturbances in hormone homeostasis. Hence, in recent decades, there has been a growing interest in endocrine disruptive chemicals. One of the alleged thyroid disrupting substances is cadmium (Cd), a ubiquitous toxic metal shown to act as a thyroid disruptor and carcinogen in both animals and humans. Multiple PubMed searches with core keywords were performed to identify and evaluate appropriate studies which revealed literature suggesting evidence for the link between exposure to Cd and histological and metabolic changes in the thyroid gland. Furthermore, Cd influence on thyroid homeostasis at the peripheral level has also been hypothesized. Both in vivo and in vitro studies revealed that a Cd exposure at environmentally relevant concentrations results in biphasic Cd dose-thyroid response relationships. Development of thyroid tumors following exposure to Cd has been studied mainly using in vitro methodologies. In the thyroid, Cd has been shown to activate or stimulate the activity of various factors, leading to increased cell proliferation and a reduction in normal apoptotic activity. Evidence establishing the association between Cd and thyroid disruption remains ambiguous, with further studies needed to elucidate the issue and improve our understanding of Cd-mediated effects on the thyroid gland.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Overview of Cadmium Thyroid Disrupting Effects and Mechanisms",
volume = "19",
number = "5",
doi = "10.3390/ijms19051501"
}

Buha, A., Matović, V., Antonijević, B., Bulat, Z., Ćurčić, M., Renieri, E. A., Tsatsakis, A., Schweitzer, A.,& Wallace, D.. (2018). Overview of Cadmium Thyroid Disrupting Effects and Mechanisms. in International Journal of Molecular Sciences
MDPI, Basel., 19(5).
https://doi.org/10.3390/ijms19051501

Buha A, Matović V, Antonijević B, Bulat Z, Ćurčić M, Renieri EA, Tsatsakis A, Schweitzer A, Wallace D. Overview of Cadmium Thyroid Disrupting Effects and Mechanisms. in International Journal of Molecular Sciences. 2018;19(5).
doi:10.3390/ijms19051501 .

Buha, Aleksandra, Matović, Vesna, Antonijević, Biljana, Bulat, Zorica, Ćurčić, Marijana, Renieri, Elisavet A., Tsatsakis, Aristidis, Schweitzer, Amie, Wallace, David, "Overview of Cadmium Thyroid Disrupting Effects and Mechanisms" in International Journal of Molecular Sciences, 19, no. 5 (2018),
https://doi.org/10.3390/ijms19051501 . .

TY  - JOUR
AU  - Renieri, Elisavet A.
AU  - Sfakianakis, Dimitris G.
AU  - Alegakis, Athanasios A.
AU  - Safenkova, Irina V.
AU  - Buha, Aleksandra
AU  - Matović, Vesna
AU  - Tzardi, Maria
AU  - Dzantiev, Boris B.
AU  - Divanach, Pascal
AU  - Kentouri, Maroudio
AU  - Tsatsakis, Aristidis
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2971
AB  - Cadmium (Cd) has proved to be associated with numerous toxic effects in aquatic organisms via waterbome exposure. With a view to investigate Cd toxicity along a broad spectrum of exposures reaching from environmental to toxic, we employed adult zebrafish (Danio rerio) for an in vivo study. A number of 10 fish per tank were placed in 40 L tanks and were exposed for 30 days to 0.0, 5.0, 25, 50, 75, 100 and 1000 mu g Cd per liter. There were 2 tanks for each Cd exposure (duplicate experiment). Mortality was recorded daily, dead fish were collected and tissue samples were obtained for histologic observation, whereas remaining tissues were stored for Cd burden determination. Surviving fish were collected at the end of the experiment. Median overall survival (OS) in days was found to be 9.0, 11.0, 8.0 and 7.0 for 25 mu g/L, 50 mu g/L, 75 mu g/L and 100 mu g/L respectively, with all of them showing mortality greater than 50%. Remarkably, fish exposed to the highest Cd concentration (1000 mu g/L) survived the longest exhibiting a mean OS of 29.2 days. Cd determination in fish tissue was conducted with an in house ICP-MS method and levels ranged from 3.1 to 29.1 ng/mg. Log Cd tissue levels were significantly correlated with the log Cd exposure levels (r = 0.535, p  lt  0.001). The highest Cd burden was determined for fish exposed to 1000 mu g Cd /L (mean = 12.2 ng/mg). Histopathology supported these results. Our findings disclose a deviation in toxic responses through the range of Cd concentrations, leading to nonlinear responses. These differentiated responses, could be linked to hormesis phenomena.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Environmental Research
T1  - Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study
VL  - 157
SP  - 173
EP  - 181
DO  - 10.1016/j.envres.2017.05.021
ER  - 

@article{
author = "Renieri, Elisavet A. and Sfakianakis, Dimitris G. and Alegakis, Athanasios A. and Safenkova, Irina V. and Buha, Aleksandra and Matović, Vesna and Tzardi, Maria and Dzantiev, Boris B. and Divanach, Pascal and Kentouri, Maroudio and Tsatsakis, Aristidis",
year = "2017",
abstract = "Cadmium (Cd) has proved to be associated with numerous toxic effects in aquatic organisms via waterbome exposure. With a view to investigate Cd toxicity along a broad spectrum of exposures reaching from environmental to toxic, we employed adult zebrafish (Danio rerio) for an in vivo study. A number of 10 fish per tank were placed in 40 L tanks and were exposed for 30 days to 0.0, 5.0, 25, 50, 75, 100 and 1000 mu g Cd per liter. There were 2 tanks for each Cd exposure (duplicate experiment). Mortality was recorded daily, dead fish were collected and tissue samples were obtained for histologic observation, whereas remaining tissues were stored for Cd burden determination. Surviving fish were collected at the end of the experiment. Median overall survival (OS) in days was found to be 9.0, 11.0, 8.0 and 7.0 for 25 mu g/L, 50 mu g/L, 75 mu g/L and 100 mu g/L respectively, with all of them showing mortality greater than 50%. Remarkably, fish exposed to the highest Cd concentration (1000 mu g/L) survived the longest exhibiting a mean OS of 29.2 days. Cd determination in fish tissue was conducted with an in house ICP-MS method and levels ranged from 3.1 to 29.1 ng/mg. Log Cd tissue levels were significantly correlated with the log Cd exposure levels (r = 0.535, p  lt  0.001). The highest Cd burden was determined for fish exposed to 1000 mu g Cd /L (mean = 12.2 ng/mg). Histopathology supported these results. Our findings disclose a deviation in toxic responses through the range of Cd concentrations, leading to nonlinear responses. These differentiated responses, could be linked to hormesis phenomena.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Environmental Research",
title = "Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study",
volume = "157",
pages = "173-181",
doi = "10.1016/j.envres.2017.05.021"
}

Renieri, E. A., Sfakianakis, D. G., Alegakis, A. A., Safenkova, I. V., Buha, A., Matović, V., Tzardi, M., Dzantiev, B. B., Divanach, P., Kentouri, M.,& Tsatsakis, A.. (2017). Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study. in Environmental Research
Academic Press Inc Elsevier Science, San Diego., 157, 173-181.
https://doi.org/10.1016/j.envres.2017.05.021

Renieri EA, Sfakianakis DG, Alegakis AA, Safenkova IV, Buha A, Matović V, Tzardi M, Dzantiev BB, Divanach P, Kentouri M, Tsatsakis A. Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study. in Environmental Research. 2017;157:173-181.
doi:10.1016/j.envres.2017.05.021 .

Renieri, Elisavet A., Sfakianakis, Dimitris G., Alegakis, Athanasios A., Safenkova, Irina V., Buha, Aleksandra, Matović, Vesna, Tzardi, Maria, Dzantiev, Boris B., Divanach, Pascal, Kentouri, Maroudio, Tsatsakis, Aristidis, "Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study" in Environmental Research, 157 (2017):173-181,
https://doi.org/10.1016/j.envres.2017.05.021 . .