TY  - CONF
AU  - Ćuruvija, Ivana
AU  - Bufan, Biljana
AU  - Đorović, Emilija
AU  - Blagojević, Veljko
AU  - Grujić-Milanović, Jelica
AU  - Marković, Milica
AU  - Đuretić, Jasmina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5132
AB  - Aims Ageing affects N-methyl-D-aspartate receptors (NMDARs), their expression and function in neuronal and non-neuronal 
cells. Contribution of NMDARs to pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been investigated 
but further study is still needed. The aim of this study was to determine whether ageing affects the role of NMDARs in EAE. 
Methods Memantine, a non-competitive NMDAR antagonist which limits pathological activity of NMDARs while sparing 
normal synaptic activity, was administered orally from day 7 after immunization to 3- and 24-month-old female Dark Agouti 
rats. The animals were sacrificed at the peak of the disease. Spinal cord mononuclear cells were analyzed by flow cytometry. 
Brain tissue was collected for biochemical analysis of redox status and RT-qPCR. Results Semiprophylactic administration of 
memantine ameliorated clinical disease course, with greater effect in aged rats. Memantine reduced the number, frequency, 
and reactivation of CD4+ T lymphocytes and increased the relative percentage of CX3CR1-expressing microglia in spinal 
cord, but to a greater extent in aged rats. Additionally, analysis of brain redox status parameters showed that memantine was 
more effective in reducing superoxide anion radical, malondialdehyde and advanced oxidation protein products in aged rats 
than in young ones. In accordance with previous findings, NMDAR inhibition by memantine decreased NADPH oxidase and 
IL-1β expression and increased the nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 expression, to a greater 
extent in aged rats. Conclusions The involvement of NMDARs in the pathogenesis of EAE was age-dependent, being more 
pronounced in aged than in young rats.
PB  - Federation of European Neuroscience Societies (FENS)
C3  - FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts
T1  - Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis
SP  - S05-250
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5132
ER  - 

@conference{
author = "Ćuruvija, Ivana and Bufan, Biljana and Đorović, Emilija and Blagojević, Veljko and Grujić-Milanović, Jelica and Marković, Milica and Đuretić, Jasmina",
year = "2022",
abstract = "Aims Ageing affects N-methyl-D-aspartate receptors (NMDARs), their expression and function in neuronal and non-neuronal 
cells. Contribution of NMDARs to pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been investigated 
but further study is still needed. The aim of this study was to determine whether ageing affects the role of NMDARs in EAE. 
Methods Memantine, a non-competitive NMDAR antagonist which limits pathological activity of NMDARs while sparing 
normal synaptic activity, was administered orally from day 7 after immunization to 3- and 24-month-old female Dark Agouti 
rats. The animals were sacrificed at the peak of the disease. Spinal cord mononuclear cells were analyzed by flow cytometry. 
Brain tissue was collected for biochemical analysis of redox status and RT-qPCR. Results Semiprophylactic administration of 
memantine ameliorated clinical disease course, with greater effect in aged rats. Memantine reduced the number, frequency, 
and reactivation of CD4+ T lymphocytes and increased the relative percentage of CX3CR1-expressing microglia in spinal 
cord, but to a greater extent in aged rats. Additionally, analysis of brain redox status parameters showed that memantine was 
more effective in reducing superoxide anion radical, malondialdehyde and advanced oxidation protein products in aged rats 
than in young ones. In accordance with previous findings, NMDAR inhibition by memantine decreased NADPH oxidase and 
IL-1β expression and increased the nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 expression, to a greater 
extent in aged rats. Conclusions The involvement of NMDARs in the pathogenesis of EAE was age-dependent, being more 
pronounced in aged than in young rats.",
publisher = "Federation of European Neuroscience Societies (FENS)",
journal = "FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts",
title = "Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis",
pages = "S05-250",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5132"
}

Ćuruvija, I., Bufan, B., Đorović, E., Blagojević, V., Grujić-Milanović, J., Marković, M.,& Đuretić, J.. (2022). Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis. in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts
Federation of European Neuroscience Societies (FENS)., S05-250.
https://hdl.handle.net/21.15107/rcub_farfar_5132

Ćuruvija I, Bufan B, Đorović E, Blagojević V, Grujić-Milanović J, Marković M, Đuretić J. Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis. in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts. 2022;:S05-250.
https://hdl.handle.net/21.15107/rcub_farfar_5132 .

Ćuruvija, Ivana, Bufan, Biljana, Đorović, Emilija, Blagojević, Veljko, Grujić-Milanović, Jelica, Marković, Milica, Đuretić, Jasmina, "Age-dependent role of NMDA receptors in experimental autoimmune encephalomyelitis" in FENS Forum 2022, 9 - 13 July, Paris, France, Book of Abstracts (2022):S05-250,
https://hdl.handle.net/21.15107/rcub_farfar_5132 .