Backović, D.

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Author's Bibliography

Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy

Backović, D.; Ignjatović, Svetlana; Rakicević, Ljiljana; Novković, Mirjana; Kusić-Tisma, Jelena; Radojković, Dragica; Strugarević, Evgenija; Čalija, Branko; Radak, Đorđe; Kovac, M.

(Elsevier, 2016)

TY  - CONF
AU  - Backović, D.
AU  - Ignjatović, Svetlana
AU  - Rakicević, Ljiljana
AU  - Novković, Mirjana
AU  - Kusić-Tisma, Jelena
AU  - Radojković, Dragica
AU  - Strugarević, Evgenija
AU  - Čalija, Branko
AU  - Radak, Đorđe
AU  - Kovac, M.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2744
AB  - Background: Carotid endarterectomy (CEA) is the standard treatment
for carotid stenosis. Dual antiplatelet therapy, including aspirin and
clopidogrel, has a potential role in reducing the risk of stroke after car-
otid surgery. However, clopidogrel high on-treatment platelet reactiv-
ity (HTPR) is quite a common phenomenon.
Aims: Our study aimed to evaluate genetic and non-genetic factors as
possible risks for clopidogrel-HTPR in patients with carotid artery
stenosis undergoing CEA.
Methods: Using multiple-electrode impedance aggregometry (MEA)
the antiplatelet effectiveness of clopidogrel was prospectively evaluated
in 112 patients (66.2   8.1 years). Measurements were made after 24 h,
7 and 30 days of clopidogrel treatment, which was introduced after
elective CEA at a dose of 75 mg daily, for at least 30 days. Clopido-
grel-HTPR was defined as an adenosine diphosphate (ADP) - throm-
bin receptor activating peptide (TRAP) platelet aggregation ratio ≥
52%. CYP2C19*2 genotyping was performed by TaqMan assay.
Logistic regression models were used to estimate predictors for low
responsiveness. The Ethics Committee of the “Dedinje” Institute for
Cardiovascular Diseases approved the research protocol. All patients
gave written informed consent prior to study inclusion.
Results: According to this specific cut-off value for our population, the
number of patients with HTPR declined from 79.5% 24 h after intro-
ducing clopidogrel to 25% after 30 days of treatment. Analysis showed
that 16/30 patients carrying the CYP2C19*2 gene variant had HTPR,
in contrast to 12/82 non-carriers of this allele (P< 0.001). Multivariate
logistic regression analysis identified the CYP2C19*2 gene variant
(OR 4.384, 95% CI 1.296-14.833, P=0.017) and high total cholesterol
(OR 2.090, 95% CI 1.263-3.459, P=0.004) as the only independent risk
factors for clopidogrel-HTPR.
Conclusions: The CYP2C19*2 gene variant and high total cholesterol
level were risk factors for clopidogrel-HTPR in patients with carotid
artery stenosis undergoing CEA.
PB  - Elsevier
C3  - Journal of Thrombosis and Haemostasis
T1  - Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy
VL  - 14
IS  - S1
SP  - 110
EP  - 110
DO  - 10.1111/jth.13325
ER  - 
@conference{
author = "Backović, D. and Ignjatović, Svetlana and Rakicević, Ljiljana and Novković, Mirjana and Kusić-Tisma, Jelena and Radojković, Dragica and Strugarević, Evgenija and Čalija, Branko and Radak, Đorđe and Kovac, M.",
year = "2016",
abstract = "Background: Carotid endarterectomy (CEA) is the standard treatment
for carotid stenosis. Dual antiplatelet therapy, including aspirin and
clopidogrel, has a potential role in reducing the risk of stroke after car-
otid surgery. However, clopidogrel high on-treatment platelet reactiv-
ity (HTPR) is quite a common phenomenon.
Aims: Our study aimed to evaluate genetic and non-genetic factors as
possible risks for clopidogrel-HTPR in patients with carotid artery
stenosis undergoing CEA.
Methods: Using multiple-electrode impedance aggregometry (MEA)
the antiplatelet effectiveness of clopidogrel was prospectively evaluated
in 112 patients (66.2   8.1 years). Measurements were made after 24 h,
7 and 30 days of clopidogrel treatment, which was introduced after
elective CEA at a dose of 75 mg daily, for at least 30 days. Clopido-
grel-HTPR was defined as an adenosine diphosphate (ADP) - throm-
bin receptor activating peptide (TRAP) platelet aggregation ratio ≥
52%. CYP2C19*2 genotyping was performed by TaqMan assay.
Logistic regression models were used to estimate predictors for low
responsiveness. The Ethics Committee of the “Dedinje” Institute for
Cardiovascular Diseases approved the research protocol. All patients
gave written informed consent prior to study inclusion.
Results: According to this specific cut-off value for our population, the
number of patients with HTPR declined from 79.5% 24 h after intro-
ducing clopidogrel to 25% after 30 days of treatment. Analysis showed
that 16/30 patients carrying the CYP2C19*2 gene variant had HTPR,
in contrast to 12/82 non-carriers of this allele (P< 0.001). Multivariate
logistic regression analysis identified the CYP2C19*2 gene variant
(OR 4.384, 95% CI 1.296-14.833, P=0.017) and high total cholesterol
(OR 2.090, 95% CI 1.263-3.459, P=0.004) as the only independent risk
factors for clopidogrel-HTPR.
Conclusions: The CYP2C19*2 gene variant and high total cholesterol
level were risk factors for clopidogrel-HTPR in patients with carotid
artery stenosis undergoing CEA.",
publisher = "Elsevier",
journal = "Journal of Thrombosis and Haemostasis",
title = "Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy",
volume = "14",
number = "S1",
pages = "110-110",
doi = "10.1111/jth.13325"
}
Backović, D., Ignjatović, S., Rakicević, L., Novković, M., Kusić-Tisma, J., Radojković, D., Strugarević, E., Čalija, B., Radak, Đ.,& Kovac, M.. (2016). Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy. in Journal of Thrombosis and Haemostasis
Elsevier., 14(S1), 110-110.
https://doi.org/10.1111/jth.13325
Backović D, Ignjatović S, Rakicević L, Novković M, Kusić-Tisma J, Radojković D, Strugarević E, Čalija B, Radak Đ, Kovac M. Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy. in Journal of Thrombosis and Haemostasis. 2016;14(S1):110-110.
doi:10.1111/jth.13325 .
Backović, D., Ignjatović, Svetlana, Rakicević, Ljiljana, Novković, Mirjana, Kusić-Tisma, Jelena, Radojković, Dragica, Strugarević, Evgenija, Čalija, Branko, Radak, Đorđe, Kovac, M., "Risk factors for clopidogrel high on-treatment platelet reactivity in patients with carotid artery stenosis undergoing endarterectomy" in Journal of Thrombosis and Haemostasis, 14, no. S1 (2016):110-110,
https://doi.org/10.1111/jth.13325 . .
3
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Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat

Pešut, Olivera; Backović, D.; Šobajić, Slađana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2005)

TY  - JOUR
AU  - Pešut, Olivera
AU  - Backović, D.
AU  - Šobajić, Slađana
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/658
AB  - Serbia is a selenium (Se) deficient area. In the food chain animals accumulate Se in tissues, thus meat is a good source of this microelement. The aim of the research is to study the effects of different forms and doses of dietary Se in pigs and broilers on muscle and liver tissue Se concentrations, as well as to assess the possibility of using selenized meat as a Se source in the Belgrade area. Pigs (24) were randomly assigned in 4 groups: control and groups supplemented with Na-selenite: 0.10, 0.30 mgSe/kg feed and selenized yeast 0.30 mgSe/kg. Chicks (42) were assigned in 7 groups supplemented with 0.05, 0.10 and 0.30 mgSe/kg feed as Na-selenite or selenized yeast. Se content in muscle and liver samples was determined by AAS. Serum Se content in 54 healthy adult subjects (13 from Barajevo and 41 from inner city area) was determined by AAS. In all supplemented broilers Se-yeast was more effective in raising muscle Se content than Na-selenite. The most efficient increase in tissue Se content in pigs was obtained with 0.30 mg Se/kg feed supplemented as Se-yeast. Plasma Se levels in man were low, being significantly lower in subjects from Barajevo compared with the inner city residents (p lt 0.05). The use of Se-enriched pork and poultry meat in quantities according to dietary habits would have beneficial health effects specially in Se deficient rural areas where home grown feedstuffs are commonly used.
AB  - Teritorija Srbije je region sa deficitom selena (Se). U stanovništvu su nivoi Se u serumu niski. Posmatrano kroz lanac ishrane, životinje akumuliraju Se u tkivima, tako da meso u ishrani predstavlja značajan izvor ovog mikroelementa. Cilj istraživanja je ispitivanje efekta različitih oblika i količina Se u ishrani svinja i brojlera na koncentraciju selena u jestivom mesu i jetri, kao i procena mogućnosti upotrebe takvog mesa u ishrani na teritorije Beograda. Uzorak od 24 svinje je podeljen u 4 grupe: kontrolnu i grupe kojima je doda-van Na-selenit u količini od 0,1 i 0,3 mg Se/kg hrane i 0,3 mg Se/kg hrane u obliku Se-kvasca. Ukupno 42 jednodnevnih pilića podeljeni su u 7 grupa: kontrolnu i grupe kojima je dodavano 0,05, 0,10 i 0,30 mg Se/kg Na-selenita ili Se-kvasca, tokom tova. Iz uzoraka mišica i jetre određivan je Se metodom atomske apsorpcione spektrofotometrije (AAS). Nivo Se u serumu određivan je AAS iz uzoraka krvi 54 zdravih odraslih ispitanika: 13 iz Barajeva i 41 iz centra Beograda. Se-kvasac je u svim dozama efikasniji u odnosu na Na-selenit u podizanju nivoa Se u mišićnom tkivu brojlera. Takođe se najefikasnije podizanje sadržaja Se u jetri i mišićima svinja postiže Se-kvascem u količini 0,3 mg Se/kg hrane. Nivoi selena u plazmi ispitanika su niski i značajno su niži kod ispitanika iz Barajeva u odnosu na ispitanike iz centra grada (p lt 0,01). Korišćenje svinjskog i pilećeg mesa obogaćenog selenom, u količinama uobičajenim domaćim dijetetskim navikama, omogućila bi pozitivne zdravstvene efekte u populaciji, značajne za ruralne regije sa deficitom Se gde se koriste pretežno prehrambeni proizvodi iz sopstvene proizvodnje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat
T1  - Suplementacija u ishrani svinja u brojlera kao način proizvodnje mesa obogaćenog selenom
VL  - 55
IS  - 5-6
SP  - 483
EP  - 492
DO  - 10.2298/AVB0506483P
ER  - 
@article{
author = "Pešut, Olivera and Backović, D. and Šobajić, Slađana",
year = "2005",
abstract = "Serbia is a selenium (Se) deficient area. In the food chain animals accumulate Se in tissues, thus meat is a good source of this microelement. The aim of the research is to study the effects of different forms and doses of dietary Se in pigs and broilers on muscle and liver tissue Se concentrations, as well as to assess the possibility of using selenized meat as a Se source in the Belgrade area. Pigs (24) were randomly assigned in 4 groups: control and groups supplemented with Na-selenite: 0.10, 0.30 mgSe/kg feed and selenized yeast 0.30 mgSe/kg. Chicks (42) were assigned in 7 groups supplemented with 0.05, 0.10 and 0.30 mgSe/kg feed as Na-selenite or selenized yeast. Se content in muscle and liver samples was determined by AAS. Serum Se content in 54 healthy adult subjects (13 from Barajevo and 41 from inner city area) was determined by AAS. In all supplemented broilers Se-yeast was more effective in raising muscle Se content than Na-selenite. The most efficient increase in tissue Se content in pigs was obtained with 0.30 mg Se/kg feed supplemented as Se-yeast. Plasma Se levels in man were low, being significantly lower in subjects from Barajevo compared with the inner city residents (p lt 0.05). The use of Se-enriched pork and poultry meat in quantities according to dietary habits would have beneficial health effects specially in Se deficient rural areas where home grown feedstuffs are commonly used., Teritorija Srbije je region sa deficitom selena (Se). U stanovništvu su nivoi Se u serumu niski. Posmatrano kroz lanac ishrane, životinje akumuliraju Se u tkivima, tako da meso u ishrani predstavlja značajan izvor ovog mikroelementa. Cilj istraživanja je ispitivanje efekta različitih oblika i količina Se u ishrani svinja i brojlera na koncentraciju selena u jestivom mesu i jetri, kao i procena mogućnosti upotrebe takvog mesa u ishrani na teritorije Beograda. Uzorak od 24 svinje je podeljen u 4 grupe: kontrolnu i grupe kojima je doda-van Na-selenit u količini od 0,1 i 0,3 mg Se/kg hrane i 0,3 mg Se/kg hrane u obliku Se-kvasca. Ukupno 42 jednodnevnih pilića podeljeni su u 7 grupa: kontrolnu i grupe kojima je dodavano 0,05, 0,10 i 0,30 mg Se/kg Na-selenita ili Se-kvasca, tokom tova. Iz uzoraka mišica i jetre određivan je Se metodom atomske apsorpcione spektrofotometrije (AAS). Nivo Se u serumu određivan je AAS iz uzoraka krvi 54 zdravih odraslih ispitanika: 13 iz Barajeva i 41 iz centra Beograda. Se-kvasac je u svim dozama efikasniji u odnosu na Na-selenit u podizanju nivoa Se u mišićnom tkivu brojlera. Takođe se najefikasnije podizanje sadržaja Se u jetri i mišićima svinja postiže Se-kvascem u količini 0,3 mg Se/kg hrane. Nivoi selena u plazmi ispitanika su niski i značajno su niži kod ispitanika iz Barajeva u odnosu na ispitanike iz centra grada (p lt 0,01). Korišćenje svinjskog i pilećeg mesa obogaćenog selenom, u količinama uobičajenim domaćim dijetetskim navikama, omogućila bi pozitivne zdravstvene efekte u populaciji, značajne za ruralne regije sa deficitom Se gde se koriste pretežno prehrambeni proizvodi iz sopstvene proizvodnje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat, Suplementacija u ishrani svinja u brojlera kao način proizvodnje mesa obogaćenog selenom",
volume = "55",
number = "5-6",
pages = "483-492",
doi = "10.2298/AVB0506483P"
}
Pešut, O., Backović, D.,& Šobajić, S.. (2005). Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 55(5-6), 483-492.
https://doi.org/10.2298/AVB0506483P
Pešut O, Backović D, Šobajić S. Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat. in Acta veterinaria. 2005;55(5-6):483-492.
doi:10.2298/AVB0506483P .
Pešut, Olivera, Backović, D., Šobajić, Slađana, "Dietary selenium supplementation of pigs and broilers as a way of producing selenium enriched meat" in Acta veterinaria, 55, no. 5-6 (2005):483-492,
https://doi.org/10.2298/AVB0506483P . .
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