TY  - JOUR
AU  - Jotić, Ana
AU  - Božić, Dragana
AU  - Milovanović, Jovica
AU  - Pavlović, Bojan
AU  - Jesić, Snežana
AU  - Pelemis, Mijomir
AU  - Novaković, Marko
AU  - Cirković, Ivana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2561
AB  - Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p  lt  0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.
PB  - Springer, New York
T2  - European Archives of Oto-Rhino-Laryngology
T1  - Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage
VL  - 273
IS  - 3
SP  - 615
EP  - 620
DO  - 10.1007/s00405-015-3607-8
ER  - 

@article{
author = "Jotić, Ana and Božić, Dragana and Milovanović, Jovica and Pavlović, Bojan and Jesić, Snežana and Pelemis, Mijomir and Novaković, Marko and Cirković, Ivana",
year = "2016",
abstract = "Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p  lt  0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.",
publisher = "Springer, New York",
journal = "European Archives of Oto-Rhino-Laryngology",
title = "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage",
volume = "273",
number = "3",
pages = "615-620",
doi = "10.1007/s00405-015-3607-8"
}

Jotić, A., Božić, D., Milovanović, J., Pavlović, B., Jesić, S., Pelemis, M., Novaković, M.,& Cirković, I.. (2016). Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology
Springer, New York., 273(3), 615-620.
https://doi.org/10.1007/s00405-015-3607-8

Jotić A, Božić D, Milovanović J, Pavlović B, Jesić S, Pelemis M, Novaković M, Cirković I. Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology. 2016;273(3):615-620.
doi:10.1007/s00405-015-3607-8 .

Jotić, Ana, Božić, Dragana, Milovanović, Jovica, Pavlović, Bojan, Jesić, Snežana, Pelemis, Mijomir, Novaković, Marko, Cirković, Ivana, "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage" in European Archives of Oto-Rhino-Laryngology, 273, no. 3 (2016):615-620,
https://doi.org/10.1007/s00405-015-3607-8 . .