TY  - JOUR
AU  - Anić-Marković, Bojana
AU  - Marinković, Aleksandar
AU  - Antić-Stanković, Jelena
AU  - Mijatović, Stefan
AU  - Cvijetić, Ilija
AU  - Simić, Milena
AU  - Aranđelović, Irena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5754
AB  - Antioxidants are promising compounds with antimicrobial activity against drug-resistant pathogens, especially when combined with conventional antimicrobials. Our study aimed to characterize the structure of nicotinamides synthesized from nicotinic acid and thiocarbohydrazones and to evaluate their antibacterial and antifungal activity. Seven nicotinic acid hydrazides (NC 1–7) were synthesized using mono-thiocarbohydrazones with hydroxyl group substituents, along with quinolone, phenolic, and pyridine rings known for their antimicrobial activity. The in vitro antimicrobial activity of NC 1–7, at concentrations ranging from 0.001 to 1 mM, was tested against Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (NCIMB 9111), and Candida albicans (ATCC 24433) using the broth microdilution method per EUCAST 2024 guidelines. Microorganism survival percentages were calculated based on optical density, and target fishing using the PharmMapper database identified potential molecular targets. The results showed that P. aeruginosa was most susceptible to the compounds, while C. albicans was the least susceptible. NC 3 significantly inhibited P. aeruginosa and K. pneumoniae growth at 0.016 mM, while higher concentrations were required for S. aureus, E. faecalis, and C. albicans. NC 5 was most effective against gram-positive bacteria at 0.03 mM. Only NC 4 completely inhibited C. albicans below 1 mM. NC 3, with the lowest concentration for 50% growth inhibition (0.016–0.064 mM), showed promising antibacterial potential against specific AMR-related proteins (bleomycin resistance protein, HTH-type transcriptional regulator QacR, and streptogramin A acetyltransferase), suggesting that this class of compounds could enhance or restore the activity of established antibiotics.
PB  - MDPI
T2  - Pharmaceutics
T1  - Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides
VL  - 16
IS  - 8
DO  - 10.3390/pharmaceutics16081084
ER  - 

@article{
author = "Anić-Marković, Bojana and Marinković, Aleksandar and Antić-Stanković, Jelena and Mijatović, Stefan and Cvijetić, Ilija and Simić, Milena and Aranđelović, Irena",
year = "2024",
abstract = "Antioxidants are promising compounds with antimicrobial activity against drug-resistant pathogens, especially when combined with conventional antimicrobials. Our study aimed to characterize the structure of nicotinamides synthesized from nicotinic acid and thiocarbohydrazones and to evaluate their antibacterial and antifungal activity. Seven nicotinic acid hydrazides (NC 1–7) were synthesized using mono-thiocarbohydrazones with hydroxyl group substituents, along with quinolone, phenolic, and pyridine rings known for their antimicrobial activity. The in vitro antimicrobial activity of NC 1–7, at concentrations ranging from 0.001 to 1 mM, was tested against Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (NCIMB 9111), and Candida albicans (ATCC 24433) using the broth microdilution method per EUCAST 2024 guidelines. Microorganism survival percentages were calculated based on optical density, and target fishing using the PharmMapper database identified potential molecular targets. The results showed that P. aeruginosa was most susceptible to the compounds, while C. albicans was the least susceptible. NC 3 significantly inhibited P. aeruginosa and K. pneumoniae growth at 0.016 mM, while higher concentrations were required for S. aureus, E. faecalis, and C. albicans. NC 5 was most effective against gram-positive bacteria at 0.03 mM. Only NC 4 completely inhibited C. albicans below 1 mM. NC 3, with the lowest concentration for 50% growth inhibition (0.016–0.064 mM), showed promising antibacterial potential against specific AMR-related proteins (bleomycin resistance protein, HTH-type transcriptional regulator QacR, and streptogramin A acetyltransferase), suggesting that this class of compounds could enhance or restore the activity of established antibiotics.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides",
volume = "16",
number = "8",
doi = "10.3390/pharmaceutics16081084"
}

Anić-Marković, B., Marinković, A., Antić-Stanković, J., Mijatović, S., Cvijetić, I., Simić, M.,& Aranđelović, I.. (2024). Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides. in Pharmaceutics
MDPI., 16(8).
https://doi.org/10.3390/pharmaceutics16081084

Anić-Marković B, Marinković A, Antić-Stanković J, Mijatović S, Cvijetić I, Simić M, Aranđelović I. Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides. in Pharmaceutics. 2024;16(8).
doi:10.3390/pharmaceutics16081084 .

Anić-Marković, Bojana, Marinković, Aleksandar, Antić-Stanković, Jelena, Mijatović, Stefan, Cvijetić, Ilija, Simić, Milena, Aranđelović, Irena, "Synthesis and Antimicrobial Activity of Newly Synthesized Nicotinamides" in Pharmaceutics, 16, no. 8 (2024),
https://doi.org/10.3390/pharmaceutics16081084 . .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Lađarević, Jelena
AU  - Pavun, Leposava
AU  - Antonijević, Biljana
AU  - Cvijetić, Ilija
AU  - Mijin, Dušan
AU  - Kostić, Mirjana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3939
AB  - The objective of this investigation was to obtain jute fabrics with enhanced sorption properties (by using simple and cost-effective alkali and oxidative modifications) and a long life cycle. The applied alkali modifications lead to hemicellulose removal and decreased the fibers’ crystallinity, both contributing to enhanced fibers’ sorption properties, i.e., water retention power and degree of fibers’ swelling up to 49 % and 70 %, respectively. A connection between cellulose polymorphs’ (cellulose I and cellulose II) contents (determined by XRD), fibers’ surface morphology (verified by FESEM), fabrics’ crimp, and capillarity of jute fabrics modified with 17.5 % NaOH was established. During the oxidative modifications, significant changes in jute fibers’ chemical composition and structure (i.e., lignin removal and more homogeneous middle lamellae) occurred which further resulted in enhanced jute fabrics’ water retention power and capillarity as well as fibers’ swelling up to 80 %, 75 %, and 54 %, compared to the raw jute, respectively. In order to move towards a circular economy and to ensure the recycling and re-use of recycled fabrics, the jute fabrics with enhanced sorption properties were evaluated as biosorbents for anthraquinone dye C. I. Acid Blue 111. The obtained results revealed that the jute fabrics’ maximum biosorption capacities for this dye ranged from 12.94 to 18.97 mg/g, while the equilibrium adsorption data were highly consistent with the Langmuir isotherm model. Moreover, based on the predicted dye pKa values, the fabric zeta potential, content of carboxyl and aldehyde groups as well as hydrogen bond intensity (determined by ATR-FTIR), a possible mechanism of the dye biosorption onto jute fabric waste was proposed.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle
VL  - 171
DO  - 10.1016/j.indcrop.2021.113913
ER  - 

@article{
author = "Ivanovska, Aleksandra and Lađarević, Jelena and Pavun, Leposava and Antonijević, Biljana and Cvijetić, Ilija and Mijin, Dušan and Kostić, Mirjana",
year = "2021",
abstract = "The objective of this investigation was to obtain jute fabrics with enhanced sorption properties (by using simple and cost-effective alkali and oxidative modifications) and a long life cycle. The applied alkali modifications lead to hemicellulose removal and decreased the fibers’ crystallinity, both contributing to enhanced fibers’ sorption properties, i.e., water retention power and degree of fibers’ swelling up to 49 % and 70 %, respectively. A connection between cellulose polymorphs’ (cellulose I and cellulose II) contents (determined by XRD), fibers’ surface morphology (verified by FESEM), fabrics’ crimp, and capillarity of jute fabrics modified with 17.5 % NaOH was established. During the oxidative modifications, significant changes in jute fibers’ chemical composition and structure (i.e., lignin removal and more homogeneous middle lamellae) occurred which further resulted in enhanced jute fabrics’ water retention power and capillarity as well as fibers’ swelling up to 80 %, 75 %, and 54 %, compared to the raw jute, respectively. In order to move towards a circular economy and to ensure the recycling and re-use of recycled fabrics, the jute fabrics with enhanced sorption properties were evaluated as biosorbents for anthraquinone dye C. I. Acid Blue 111. The obtained results revealed that the jute fabrics’ maximum biosorption capacities for this dye ranged from 12.94 to 18.97 mg/g, while the equilibrium adsorption data were highly consistent with the Langmuir isotherm model. Moreover, based on the predicted dye pKa values, the fabric zeta potential, content of carboxyl and aldehyde groups as well as hydrogen bond intensity (determined by ATR-FTIR), a possible mechanism of the dye biosorption onto jute fabric waste was proposed.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle",
volume = "171",
doi = "10.1016/j.indcrop.2021.113913"
}

Ivanovska, A., Lađarević, J., Pavun, L., Antonijević, B., Cvijetić, I., Mijin, D.,& Kostić, M.. (2021). Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle. in Industrial Crops and Products
Elsevier B.V.., 171.
https://doi.org/10.1016/j.indcrop.2021.113913

Ivanovska A, Lađarević J, Pavun L, Antonijević B, Cvijetić I, Mijin D, Kostić M. Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle. in Industrial Crops and Products. 2021;171.
doi:10.1016/j.indcrop.2021.113913 .

Ivanovska, Aleksandra, Lađarević, Jelena, Pavun, Leposava, Antonijević, Biljana, Cvijetić, Ilija, Mijin, Dušan, Kostić, Mirjana, "Obtaining jute fabrics with enhanced sorption properties and “closing the loop” of their lifecycle" in Industrial Crops and Products, 171 (2021),
https://doi.org/10.1016/j.indcrop.2021.113913 . .

TY  - JOUR
AU  - Erić, Slavica
AU  - Cvijetić, Ilija
AU  - Zloh, Mire
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3929
AB  - Metabolism of sulfur (sulfur assimilation pathway, SAP) is one of the key pathways for the pathogenesis and survival of persistant bacteria, such as Mycobacterium tuberculosis (Mtb), in the latent period. Adenosine 5'-phosphosulfate reductase (APSR) is an important enzyme involved in the SAP, absent from the human body, so it might represent a valid target for development of new antituberculosis drugs. This work aimed to develop 3D-QSAR model based on the crystal structure of APSR from Pseudomonas aeruginosa, which shows high degree of homology with APSR from Mtb, in complex with its substrate, adenosine 5'-phosphosulfate (APS). 3D-QSAR model was built from a set of 16 nucleotide analogues of APS using alignment-independent descriptors derived from molecular interaction fields (MIF). The model improves the understanding of the key characteristics of molecules necessary for the interaction with target, and enables the rational design of novel small molecule inhibitors of Mtb APSR.
AB  - Метаболизам  сумпора (пут асимилације  сумпора, SAP) један је од кључних путева  за  патогенезу  и  преживљавање  Mycobacterium  tuberculosis  (Mtb)  у  латентном  периоду.  Аденозин  5'-фосфосфат  редуктаза  (APSR)  је  значајан  ензим  који  је  укључен  у  SAP,  не  налази се у људском организму и може бити валиднo циљно место за развој нових анти- туберкулотика.  Циљ  овог  рада  је  развој  3D-QSAR  модела  који  се  заснива  на  кристалној  структури APSR из Pseudomonas aeruginosa, који има висок степен хомологије са APSR из  Mtb, у комплексу са супстратом, аденозин 5'-фосфoсулфатом (APS). 3D-QSAR модел је  постављен коришћењем сета 16 нуклеотидних аналога APS применом дескриптора неза- висних од полазних тачака, изведених из поља молекуларних интеракција (MIF). Модел  служи  за  боље  разумевање  кључних  карактеристика  молекула  неопходних  за  интерак- цију са циљним местом, у сврху рационалног дизајнирања малих молекула, инхибитора  APSR из Mtb.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - 3D-QSAR study of adenosine 5'-phosphosulfate (APS) analouges as ligands for APS reductase
T1  - 3D-QSAR студија аналога аденозин 5'-фосфосулфата (APS) као лиганда за APS редуктазу
VL  - 86
IS  - 6
SP  - 561
EP  - 570
DO  - 10.2298/JSC201128015E
ER  - 

@article{
author = "Erić, Slavica and Cvijetić, Ilija and Zloh, Mire",
year = "2021",
abstract = "Metabolism of sulfur (sulfur assimilation pathway, SAP) is one of the key pathways for the pathogenesis and survival of persistant bacteria, such as Mycobacterium tuberculosis (Mtb), in the latent period. Adenosine 5'-phosphosulfate reductase (APSR) is an important enzyme involved in the SAP, absent from the human body, so it might represent a valid target for development of new antituberculosis drugs. This work aimed to develop 3D-QSAR model based on the crystal structure of APSR from Pseudomonas aeruginosa, which shows high degree of homology with APSR from Mtb, in complex with its substrate, adenosine 5'-phosphosulfate (APS). 3D-QSAR model was built from a set of 16 nucleotide analogues of APS using alignment-independent descriptors derived from molecular interaction fields (MIF). The model improves the understanding of the key characteristics of molecules necessary for the interaction with target, and enables the rational design of novel small molecule inhibitors of Mtb APSR., Метаболизам  сумпора (пут асимилације  сумпора, SAP) један је од кључних путева  за  патогенезу  и  преживљавање  Mycobacterium  tuberculosis  (Mtb)  у  латентном  периоду.  Аденозин  5'-фосфосфат  редуктаза  (APSR)  је  значајан  ензим  који  је  укључен  у  SAP,  не  налази се у људском организму и може бити валиднo циљно место за развој нових анти- туберкулотика.  Циљ  овог  рада  је  развој  3D-QSAR  модела  који  се  заснива  на  кристалној  структури APSR из Pseudomonas aeruginosa, који има висок степен хомологије са APSR из  Mtb, у комплексу са супстратом, аденозин 5'-фосфoсулфатом (APS). 3D-QSAR модел је  постављен коришћењем сета 16 нуклеотидних аналога APS применом дескриптора неза- висних од полазних тачака, изведених из поља молекуларних интеракција (MIF). Модел  служи  за  боље  разумевање  кључних  карактеристика  молекула  неопходних  за  интерак- цију са циљним местом, у сврху рационалног дизајнирања малих молекула, инхибитора  APSR из Mtb.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "3D-QSAR study of adenosine 5'-phosphosulfate (APS) analouges as ligands for APS reductase, 3D-QSAR студија аналога аденозин 5'-фосфосулфата (APS) као лиганда за APS редуктазу",
volume = "86",
number = "6",
pages = "561-570",
doi = "10.2298/JSC201128015E"
}

Erić, S., Cvijetić, I.,& Zloh, M.. (2021). 3D-QSAR study of adenosine 5'-phosphosulfate (APS) analouges as ligands for APS reductase. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society., 86(6), 561-570.
https://doi.org/10.2298/JSC201128015E

Erić S, Cvijetić I, Zloh M. 3D-QSAR study of adenosine 5'-phosphosulfate (APS) analouges as ligands for APS reductase. in Journal of the Serbian Chemical Society. 2021;86(6):561-570.
doi:10.2298/JSC201128015E .

Erić, Slavica, Cvijetić, Ilija, Zloh, Mire, "3D-QSAR study of adenosine 5'-phosphosulfate (APS) analouges as ligands for APS reductase" in Journal of the Serbian Chemical Society, 86, no. 6 (2021):561-570,
https://doi.org/10.2298/JSC201128015E . .