TY  - JOUR
AU  - Dobrić, Milan
AU  - Giga, Vojislav
AU  - Beleslin, Branko
AU  - Ignjatović, Svetlana
AU  - Paunović, Ivana
AU  - Stepanović, Jelena M.
AU  - Đorđević-Dikić, Ana
AU  - Kostić, Jelena
AU  - Nedeljković, Ivana
AU  - Nedeljković, Milan
AU  - Tesić, Milorad
AU  - Dajak, Marijana
AU  - Ostojić, Miodrag
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1868
AB  - Background: Glycogen phosphorylase BB (GPBB) is released from cardiac cells during myocyte damage. Previous studies have shown contradictory results regarding the relation of enzyme release and reversible myocardial ischemia. The aim of this study was to determine the plasma kinetics of GPBB as a response to the exercise stress echocardiographic test (ESET), and to define the relationship between myocardial ischemia and enzyme plasma concentrations. Methods: We studied 46 consecutive patients undergoing ESET, with recent coronary angiography. In all patients, a submaximal stress echo test according to Bruce protocol was performed. Concentration of GPBB was measured in peripheral blood that was sampled 5 min before and 10, 30 and 60 min after ESET. Results: There was significant increase of GPBB concentration after the test (p=0.021). Significant increase was detected 30 min (34.9% increase, p=0.021) and 60 min (34.5% increase, p=0.016) after ESET. There was no significant effect of myocardial ischemia on GPBB concentrations (p=0.126), and no significant interaction between sampling intervals and myocardial ischemia, suggesting a similar release profile of GPBB in ischemic and non-ischemic conditions (p=0.558). Patients in whom ESET was terminated later (stages 4 or 5 of standard Bruce protocol; n=13) had higher GPBB concentrations than patients who terminated ESET earlier (stages 1, 2 or 3; n=33) (p=0.049). Baseline GPBB concentration was not correlated to any of the patients' demographic, clinical and hemodynamic characteristics. Conclusions: GPBB plasma concentration increases after ESET, and it is not related to inducible myocardial ischemia. However, it seems that GPBB release during ESET might be related to exercise load/duration.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test
VL  - 51
IS  - 10
SP  - 2029
EP  - 2035
DO  - 10.1515/cclm-2013-0109
ER  - 

@article{
author = "Dobrić, Milan and Giga, Vojislav and Beleslin, Branko and Ignjatović, Svetlana and Paunović, Ivana and Stepanović, Jelena M. and Đorđević-Dikić, Ana and Kostić, Jelena and Nedeljković, Ivana and Nedeljković, Milan and Tesić, Milorad and Dajak, Marijana and Ostojić, Miodrag",
year = "2013",
abstract = "Background: Glycogen phosphorylase BB (GPBB) is released from cardiac cells during myocyte damage. Previous studies have shown contradictory results regarding the relation of enzyme release and reversible myocardial ischemia. The aim of this study was to determine the plasma kinetics of GPBB as a response to the exercise stress echocardiographic test (ESET), and to define the relationship between myocardial ischemia and enzyme plasma concentrations. Methods: We studied 46 consecutive patients undergoing ESET, with recent coronary angiography. In all patients, a submaximal stress echo test according to Bruce protocol was performed. Concentration of GPBB was measured in peripheral blood that was sampled 5 min before and 10, 30 and 60 min after ESET. Results: There was significant increase of GPBB concentration after the test (p=0.021). Significant increase was detected 30 min (34.9% increase, p=0.021) and 60 min (34.5% increase, p=0.016) after ESET. There was no significant effect of myocardial ischemia on GPBB concentrations (p=0.126), and no significant interaction between sampling intervals and myocardial ischemia, suggesting a similar release profile of GPBB in ischemic and non-ischemic conditions (p=0.558). Patients in whom ESET was terminated later (stages 4 or 5 of standard Bruce protocol; n=13) had higher GPBB concentrations than patients who terminated ESET earlier (stages 1, 2 or 3; n=33) (p=0.049). Baseline GPBB concentration was not correlated to any of the patients' demographic, clinical and hemodynamic characteristics. Conclusions: GPBB plasma concentration increases after ESET, and it is not related to inducible myocardial ischemia. However, it seems that GPBB release during ESET might be related to exercise load/duration.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test",
volume = "51",
number = "10",
pages = "2029-2035",
doi = "10.1515/cclm-2013-0109"
}

Dobrić, M., Giga, V., Beleslin, B., Ignjatović, S., Paunović, I., Stepanović, J. M., Đorđević-Dikić, A., Kostić, J., Nedeljković, I., Nedeljković, M., Tesić, M., Dajak, M.,& Ostojić, M.. (2013). Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 51(10), 2029-2035.
https://doi.org/10.1515/cclm-2013-0109

Dobrić M, Giga V, Beleslin B, Ignjatović S, Paunović I, Stepanović JM, Đorđević-Dikić A, Kostić J, Nedeljković I, Nedeljković M, Tesić M, Dajak M, Ostojić M. Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test. in Clinical Chemistry and Laboratory Medicine. 2013;51(10):2029-2035.
doi:10.1515/cclm-2013-0109 .

Dobrić, Milan, Giga, Vojislav, Beleslin, Branko, Ignjatović, Svetlana, Paunović, Ivana, Stepanović, Jelena M., Đorđević-Dikić, Ana, Kostić, Jelena, Nedeljković, Ivana, Nedeljković, Milan, Tesić, Milorad, Dajak, Marijana, Ostojić, Miodrag, "Glycogen phosphorylase isoenzyme BB plasma kinetics is not related to myocardial ischemia induced by exercise stress echo test" in Clinical Chemistry and Laboratory Medicine, 51, no. 10 (2013):2029-2035,
https://doi.org/10.1515/cclm-2013-0109 . .

TY  - JOUR
AU  - Stepanović-Petrović, Radica
AU  - Savić, Vladimir
AU  - Tomić, Maja
AU  - Tokić-Vujošević, Zorana
AU  - Simić, Milena
AU  - Stepanović, Jelena M.
AU  - Jokanović, Milan
AU  - Micov, Ana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1337
AB  - Background/Aims: 5-Ketoximeisosorbide-2-mononitrate (50-IS-2-MN) was synthesized and its pharmacological and toxicological characteristics were examined and compared with its parent drug, isosorbide-5-mononitrate (IS-5-MN, CAS 16051-77-7), and its diastereoisomer 2-ketoximeisosorbide-5-mononitrate. Methods: Vasorelaxation was studied on phenylephrine-precontracted rat superior mesenteric artery rings in organ bath procedure. In some rings, the endothelium was mechanically removed. In vitro tolerance was induced by treating the precontracted rings with maximal concentrations of the parent drug and the ketoximes, and after washing out, the procedure was repeated for two times. Furthermore, rats were treated with a single oral dose (1000 mg/kg) of 50-IS-2-MN and 20-IS-5-MN. Results: After a phenylephrine-induced contraction, 50-IS-2-MN (10(-8)-10(-4) mol/l) caused a concentration-dependent relaxation of the rat superior mesenteric artery that was strongly potentiated after the removal of the vascular endothelium. In preparations with or without endothelium, 50-IS-2-MN was a more potent relaxant than either the parent compound or its isomer. The mechanism of the relaxant effect of 50-IS-2-MN involves the activated soluble guanylyl cyclase-cyclic GMP pathway. Hydralazine (10(-5) mol/l), a strong antioxidant, ameliorated tolerance to IS-5-MN, but did not affect the absence of tolerance to either ketoxime. The minimum lethal dose in rat for 50-IS-2-MN and 20-IS-5-MN was greater than 1000 mg/kg. Conclusion: These results suggest that the modification of the configuration at the ester carbon of IS-5-MN contributes to more potent and tolerance-devoid activity on the rat superior mesenteric artery.
PB  - ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf
T2  - Arzneimittelforschung - Drug Research
T1  - Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery
VL  - 60
IS  - 4
SP  - 189
EP  - 197
DO  - 10.1055/s-0031-1296272
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1337
ER  - 

@article{
author = "Stepanović-Petrović, Radica and Savić, Vladimir and Tomić, Maja and Tokić-Vujošević, Zorana and Simić, Milena and Stepanović, Jelena M. and Jokanović, Milan and Micov, Ana",
year = "2010",
abstract = "Background/Aims: 5-Ketoximeisosorbide-2-mononitrate (50-IS-2-MN) was synthesized and its pharmacological and toxicological characteristics were examined and compared with its parent drug, isosorbide-5-mononitrate (IS-5-MN, CAS 16051-77-7), and its diastereoisomer 2-ketoximeisosorbide-5-mononitrate. Methods: Vasorelaxation was studied on phenylephrine-precontracted rat superior mesenteric artery rings in organ bath procedure. In some rings, the endothelium was mechanically removed. In vitro tolerance was induced by treating the precontracted rings with maximal concentrations of the parent drug and the ketoximes, and after washing out, the procedure was repeated for two times. Furthermore, rats were treated with a single oral dose (1000 mg/kg) of 50-IS-2-MN and 20-IS-5-MN. Results: After a phenylephrine-induced contraction, 50-IS-2-MN (10(-8)-10(-4) mol/l) caused a concentration-dependent relaxation of the rat superior mesenteric artery that was strongly potentiated after the removal of the vascular endothelium. In preparations with or without endothelium, 50-IS-2-MN was a more potent relaxant than either the parent compound or its isomer. The mechanism of the relaxant effect of 50-IS-2-MN involves the activated soluble guanylyl cyclase-cyclic GMP pathway. Hydralazine (10(-5) mol/l), a strong antioxidant, ameliorated tolerance to IS-5-MN, but did not affect the absence of tolerance to either ketoxime. The minimum lethal dose in rat for 50-IS-2-MN and 20-IS-5-MN was greater than 1000 mg/kg. Conclusion: These results suggest that the modification of the configuration at the ester carbon of IS-5-MN contributes to more potent and tolerance-devoid activity on the rat superior mesenteric artery.",
publisher = "ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf",
journal = "Arzneimittelforschung - Drug Research",
title = "Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery",
volume = "60",
number = "4",
pages = "189-197",
doi = "10.1055/s-0031-1296272",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1337"
}

Stepanović-Petrović, R., Savić, V., Tomić, M., Tokić-Vujošević, Z., Simić, M., Stepanović, J. M., Jokanović, M.,& Micov, A.. (2010). Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery. in Arzneimittelforschung - Drug Research
ECV-Editio Cantor Verlag Medizin Naturwissenschaften, Aulendorf., 60(4), 189-197.
https://doi.org/10.1055/s-0031-1296272
https://hdl.handle.net/21.15107/rcub_farfar_1337

Stepanović-Petrović R, Savić V, Tomić M, Tokić-Vujošević Z, Simić M, Stepanović JM, Jokanović M, Micov A. Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery. in Arzneimittelforschung - Drug Research. 2010;60(4):189-197.
doi:10.1055/s-0031-1296272
https://hdl.handle.net/21.15107/rcub_farfar_1337 .

Stepanović-Petrović, Radica, Savić, Vladimir, Tomić, Maja, Tokić-Vujošević, Zorana, Simić, Milena, Stepanović, Jelena M., Jokanović, Milan, Micov, Ana, "Comparison of vasorelaxant effect and tolerance profile of a novel isosorbide-5-mononitrate derivative with its stereoisomer and parent drug on rat mesenteric artery" in Arzneimittelforschung - Drug Research, 60, no. 4 (2010):189-197,
https://doi.org/10.1055/s-0031-1296272 .,
https://hdl.handle.net/21.15107/rcub_farfar_1337 .