TY  - JOUR
AU  - Malenović, Anđelija
AU  - Jovanović, Marko
AU  - Petrović, Sonja
AU  - Kostić, Nada
AU  - Vemić, Ana
AU  - Jančić-Stojanović, Biljana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1749
AB  - In this article, the multiobjective optimization of reversed-phase high-performance liquid chromatography (RP-HPLC) method for the analysis of folic acid and its two impurities, p-aminobenzoic acid and N-(4-aminobenzoyl)-L-glutamic acid, is presented. During the preliminary study, the independent variables whose impact should be further examined in the optimization were defined (acetonitrile content in the mobile phase, molarity of sodium 1-heptanesulfonate in the aqueous phase, and pH of the aqueous phase). The face-centered central composite design was chosen for the method optimization. As the dependent variables, the separation between the impurities and the retention factor of folic acid were followed. The analysis of variance (ANOVA) test was done, and good correlation among results was confirmed. For the more accurate and reliable optimization, Derringer's desirability function was applied. On the basis of restrictions adopted for the selected targets, the resulting optimal composition of the mobile phase was acetonitrile -3 mM sodium 1-heptanesulfonate (5.3:94.7 V/V), pH of the aqueous phase adjusted to 2.1 with the concentrated ortho-phosphoric acid. Furthermore, the method was subjected to method validation. It proved to be reliable and suitable for the routine analysis of investigated substances in active pharmaceutical ingredients, as well as in pharmaceutical preparations.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Instrumentation Science & Technology
T1  - Optimization of liquid chromatographic method for the separation of folic acid and its two impurities
VL  - 40
IS  - 2-3
SP  - 138
EP  - 149
DO  - 10.1080/10739149.2011.651670
ER  - 

@article{
author = "Malenović, Anđelija and Jovanović, Marko and Petrović, Sonja and Kostić, Nada and Vemić, Ana and Jančić-Stojanović, Biljana",
year = "2012",
abstract = "In this article, the multiobjective optimization of reversed-phase high-performance liquid chromatography (RP-HPLC) method for the analysis of folic acid and its two impurities, p-aminobenzoic acid and N-(4-aminobenzoyl)-L-glutamic acid, is presented. During the preliminary study, the independent variables whose impact should be further examined in the optimization were defined (acetonitrile content in the mobile phase, molarity of sodium 1-heptanesulfonate in the aqueous phase, and pH of the aqueous phase). The face-centered central composite design was chosen for the method optimization. As the dependent variables, the separation between the impurities and the retention factor of folic acid were followed. The analysis of variance (ANOVA) test was done, and good correlation among results was confirmed. For the more accurate and reliable optimization, Derringer's desirability function was applied. On the basis of restrictions adopted for the selected targets, the resulting optimal composition of the mobile phase was acetonitrile -3 mM sodium 1-heptanesulfonate (5.3:94.7 V/V), pH of the aqueous phase adjusted to 2.1 with the concentrated ortho-phosphoric acid. Furthermore, the method was subjected to method validation. It proved to be reliable and suitable for the routine analysis of investigated substances in active pharmaceutical ingredients, as well as in pharmaceutical preparations.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Instrumentation Science & Technology",
title = "Optimization of liquid chromatographic method for the separation of folic acid and its two impurities",
volume = "40",
number = "2-3",
pages = "138-149",
doi = "10.1080/10739149.2011.651670"
}

Malenović, A., Jovanović, M., Petrović, S., Kostić, N., Vemić, A.,& Jančić-Stojanović, B.. (2012). Optimization of liquid chromatographic method for the separation of folic acid and its two impurities. in Instrumentation Science & Technology
Taylor & Francis Inc, Philadelphia., 40(2-3), 138-149.
https://doi.org/10.1080/10739149.2011.651670

Malenović A, Jovanović M, Petrović S, Kostić N, Vemić A, Jančić-Stojanović B. Optimization of liquid chromatographic method for the separation of folic acid and its two impurities. in Instrumentation Science & Technology. 2012;40(2-3):138-149.
doi:10.1080/10739149.2011.651670 .

Malenović, Anđelija, Jovanović, Marko, Petrović, Sonja, Kostić, Nada, Vemić, Ana, Jančić-Stojanović, Biljana, "Optimization of liquid chromatographic method for the separation of folic acid and its two impurities" in Instrumentation Science & Technology, 40, no. 2-3 (2012):138-149,
https://doi.org/10.1080/10739149.2011.651670 . .