TY  - CONF
AU  - Bojović, Dragica
AU  - Šoškić, Miomir
AU  - Ljumović, Iva
AU  - Žugić, Ana
AU  - Milenković, Marina
AU  - Tadić, Vanja
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5330
AB  - Laurus nobilis L., Lauraceae, u narodu poznat kao lovor, je cvetni, zimzeleni žbun koji
samoniklo raste u većem delu Crne Gore, a često se uzgaja i koristi kao aromatična, začinska i lekovita
biljka. Cilj ovog istraživanja je bio da se analizira hemijski sastav etarskog ulja (EU) ploda i lista, a
zatim testira antibakterijska i antifungalna aktivnost EU na sledećim sojevima: Staphylococcus aureus
ATCC25923, Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 Escherichia coli
ATCC25922, Klebsiella pneumoniae ATCC13083, Salmonella Abony NCTC6017, Pseudomonas
aeruginosa ATCC27853 i Candida albicans ATCC10231. Ispitivanje kvalitativnog i kvantitativnog
sastava etarskih ulja lista i plodova lovora izvršeno je gasnohromatografskom tehnikom spregnutom
sa masenom spektrometrijom (GC-MS). Utvrđeno je da je etarsko ulje ploda bogato 1,8-cineolom
(33,3%), α-terpenil acetatom (7,0%), α-pinenom (5,8%), β-elemenom (5,7%), sabinenom (5,3%), β-
pinenom (4,0%), borneolom (2,1%) i terpinen-4-olom (2,1%). Najvažnije komponente
identi�ikovane u EU listova su 1,8-cineol (35,1%), α-terpinil acetat (10,4%) i linalol (7,6%), između
ostalih, a u skladu su sa prethodno dobijenim literaturnim podacima (1). Dodatno, ispitivana su
antibakterijska i antifungalna svojstva EU lista i ploda lovora bujon mikrodilucionom metodom. EU
ploda je pokazao antibakterijsko i antifungalno svojstvo protiv skoro svih testiranih sojeva
mikroorganizama, dok je EU lista inhibiralo sledeće sojeve: Staphylococcus aureus ATCC25923,
Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 i Candida albicans ATCC10231.
Escherichia coli ATCC25922 je bakterijski soj koji je pokazao najveću otpornost na etarska ulja lovora,
kako ploda, tako i lista. Dobijeni rezultati za etarska ulja lovora i 1,8 -cineol, koji je njihov glavni
sastojak, su u skladu sa prethodnim studijama (2).
AB  - Laurus nobilis L., Lauraceae, popularly known as laurel, is a flowering, evergreen bush which
grows wildly in most parts of Montenegro, and is often cultivated and used as an aromatic, flavoring
spice and medicinal plant. The aim of this research was to analyze the chemical composition of the
essential oil (EO) of fruit and leaf, and afterwards antibacterial and antifungal activity of EOs on the
following strains: Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC29212, Bacillus
subtilis ATCC6633 Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC13083, Salmonella Abony
NCTC6017, Pseudomonas aeruginosa ATCC27853 i Candida albicans ATCC10231. The qualitative and
quantitative composition of the EOs of fruit and leaves was carried out by using a gas
chromatographic analysis coupled with mass spectrometry (GC-MS). The EO of fruit was found to be
rich 1,8-cineole (33.3%), α-terpinyl acetate (7.0%), α-pinene (5.8%), β-elemene (5.7%), sabinene
(5.3%), β-pinene (4.0%), borneol (2.1%) and terpinene-4-ol (2.1%). The most important
components identified in the leaves EO were 1,8-cineol (35.1%), α-terpinyl acetate (10.4%) and
linalool (7.6%), among others, are in line with previous literature reports (1). In addition, the
antibacterial and antifungal properties of leaf and fruit laurel EOs were examined according to the
broth microdilution method. The fruit EO showed antibacterial and antifungal activities against
almost all strains of the microorganisms tested, whereas the leaf EO was able to inhibit:
Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 and
Candida albicans ATCC10231. Escherichia coli ATCC25922 was bacterial strain that showed the
highest resistance to both tested laurel EOs. The results obtained herein for laurel EO and 1,8-cineole
as its main constituent are in accordance with previous studies (2).
PB  - Savez farmaceutskih udruženja Srbije
C3  - Arhiv za farmaciju
T1  - Hemijska analiza i antimikrobna aktivnost etarskih ulja samoniklog lovora sa područja Nacionalnog parka Skadarsko jezero, Crna Gora
T1  - Chemical analysis and antimicrobial activity of essential oils from wild laurel of National park Skadar lake, Montenegro
VL  - 73
IS  - Suppl. 4
SP  - S85
EP  - S86
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5330
ER  - 

@conference{
author = "Bojović, Dragica and Šoškić, Miomir and Ljumović, Iva and Žugić, Ana and Milenković, Marina and Tadić, Vanja",
year = "2023",
abstract = "Laurus nobilis L., Lauraceae, u narodu poznat kao lovor, je cvetni, zimzeleni žbun koji
samoniklo raste u većem delu Crne Gore, a često se uzgaja i koristi kao aromatična, začinska i lekovita
biljka. Cilj ovog istraživanja je bio da se analizira hemijski sastav etarskog ulja (EU) ploda i lista, a
zatim testira antibakterijska i antifungalna aktivnost EU na sledećim sojevima: Staphylococcus aureus
ATCC25923, Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 Escherichia coli
ATCC25922, Klebsiella pneumoniae ATCC13083, Salmonella Abony NCTC6017, Pseudomonas
aeruginosa ATCC27853 i Candida albicans ATCC10231. Ispitivanje kvalitativnog i kvantitativnog
sastava etarskih ulja lista i plodova lovora izvršeno je gasnohromatografskom tehnikom spregnutom
sa masenom spektrometrijom (GC-MS). Utvrđeno je da je etarsko ulje ploda bogato 1,8-cineolom
(33,3%), α-terpenil acetatom (7,0%), α-pinenom (5,8%), β-elemenom (5,7%), sabinenom (5,3%), β-
pinenom (4,0%), borneolom (2,1%) i terpinen-4-olom (2,1%). Najvažnije komponente
identi�ikovane u EU listova su 1,8-cineol (35,1%), α-terpinil acetat (10,4%) i linalol (7,6%), između
ostalih, a u skladu su sa prethodno dobijenim literaturnim podacima (1). Dodatno, ispitivana su
antibakterijska i antifungalna svojstva EU lista i ploda lovora bujon mikrodilucionom metodom. EU
ploda je pokazao antibakterijsko i antifungalno svojstvo protiv skoro svih testiranih sojeva
mikroorganizama, dok je EU lista inhibiralo sledeće sojeve: Staphylococcus aureus ATCC25923,
Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 i Candida albicans ATCC10231.
Escherichia coli ATCC25922 je bakterijski soj koji je pokazao najveću otpornost na etarska ulja lovora,
kako ploda, tako i lista. Dobijeni rezultati za etarska ulja lovora i 1,8 -cineol, koji je njihov glavni
sastojak, su u skladu sa prethodnim studijama (2)., Laurus nobilis L., Lauraceae, popularly known as laurel, is a flowering, evergreen bush which
grows wildly in most parts of Montenegro, and is often cultivated and used as an aromatic, flavoring
spice and medicinal plant. The aim of this research was to analyze the chemical composition of the
essential oil (EO) of fruit and leaf, and afterwards antibacterial and antifungal activity of EOs on the
following strains: Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC29212, Bacillus
subtilis ATCC6633 Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC13083, Salmonella Abony
NCTC6017, Pseudomonas aeruginosa ATCC27853 i Candida albicans ATCC10231. The qualitative and
quantitative composition of the EOs of fruit and leaves was carried out by using a gas
chromatographic analysis coupled with mass spectrometry (GC-MS). The EO of fruit was found to be
rich 1,8-cineole (33.3%), α-terpinyl acetate (7.0%), α-pinene (5.8%), β-elemene (5.7%), sabinene
(5.3%), β-pinene (4.0%), borneol (2.1%) and terpinene-4-ol (2.1%). The most important
components identified in the leaves EO were 1,8-cineol (35.1%), α-terpinyl acetate (10.4%) and
linalool (7.6%), among others, are in line with previous literature reports (1). In addition, the
antibacterial and antifungal properties of leaf and fruit laurel EOs were examined according to the
broth microdilution method. The fruit EO showed antibacterial and antifungal activities against
almost all strains of the microorganisms tested, whereas the leaf EO was able to inhibit:
Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC29212, Bacillus subtilis ATCC6633 and
Candida albicans ATCC10231. Escherichia coli ATCC25922 was bacterial strain that showed the
highest resistance to both tested laurel EOs. The results obtained herein for laurel EO and 1,8-cineole
as its main constituent are in accordance with previous studies (2).",
publisher = "Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "Hemijska analiza i antimikrobna aktivnost etarskih ulja samoniklog lovora sa područja Nacionalnog parka Skadarsko jezero, Crna Gora, Chemical analysis and antimicrobial activity of essential oils from wild laurel of National park Skadar lake, Montenegro",
volume = "73",
number = "Suppl. 4",
pages = "S85-S86",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5330"
}

Bojović, D., Šoškić, M., Ljumović, I., Žugić, A., Milenković, M.,& Tadić, V.. (2023). Hemijska analiza i antimikrobna aktivnost etarskih ulja samoniklog lovora sa područja Nacionalnog parka Skadarsko jezero, Crna Gora. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije., 73(Suppl. 4), S85-S86.
https://hdl.handle.net/21.15107/rcub_farfar_5330

Bojović D, Šoškić M, Ljumović I, Žugić A, Milenković M, Tadić V. Hemijska analiza i antimikrobna aktivnost etarskih ulja samoniklog lovora sa područja Nacionalnog parka Skadarsko jezero, Crna Gora. in Arhiv za farmaciju. 2023;73(Suppl. 4):S85-S86.
https://hdl.handle.net/21.15107/rcub_farfar_5330 .

Bojović, Dragica, Šoškić, Miomir, Ljumović, Iva, Žugić, Ana, Milenković, Marina, Tadić, Vanja, "Hemijska analiza i antimikrobna aktivnost etarskih ulja samoniklog lovora sa područja Nacionalnog parka Skadarsko jezero, Crna Gora" in Arhiv za farmaciju, 73, no. Suppl. 4 (2023):S85-S86,
https://hdl.handle.net/21.15107/rcub_farfar_5330 .

TY  - JOUR
AU  - Žugić, Ana
AU  - Tadić, Vanja
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3545
AB  - Usnic acid is one of the most investigated lichen secondary metabolites, with several proven biological properties with potential medical relevance. However, its unfavorable physico-chemical properties, as well as observed hepatotoxicity, have discouraged wide-range utilization of usnic acid as a promising therapeutic agent. In accordance with the growing research interest in the development of nanotechnology, especially in the arena of preparations based on natural sources of medicinal compounds, usnic acid incorporated into nano-and microsized colloidal carriers has been a subject of a large number of publications. Therefore, this review discusses the overall results of the studies dealing with usnic acid encapsulated into lipid-based, polymeric and nonorganic micro-and/or nanocarriers, as potential drug delivery systems for this natural compound, in an attempt to introduce its usage as a potential antitumor, antimicrobial, wound-healing, antioxidative and anti-inflammatory drug.
PB  - MDPI
T2  - Pharmaceutics
T1  - Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature
VL  - 12
IS  - 2
DO  - 10.3390/pharmaceutics12020156
ER  - 

@article{
author = "Žugić, Ana and Tadić, Vanja and Savić, Snežana",
year = "2020",
abstract = "Usnic acid is one of the most investigated lichen secondary metabolites, with several proven biological properties with potential medical relevance. However, its unfavorable physico-chemical properties, as well as observed hepatotoxicity, have discouraged wide-range utilization of usnic acid as a promising therapeutic agent. In accordance with the growing research interest in the development of nanotechnology, especially in the arena of preparations based on natural sources of medicinal compounds, usnic acid incorporated into nano-and microsized colloidal carriers has been a subject of a large number of publications. Therefore, this review discusses the overall results of the studies dealing with usnic acid encapsulated into lipid-based, polymeric and nonorganic micro-and/or nanocarriers, as potential drug delivery systems for this natural compound, in an attempt to introduce its usage as a potential antitumor, antimicrobial, wound-healing, antioxidative and anti-inflammatory drug.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature",
volume = "12",
number = "2",
doi = "10.3390/pharmaceutics12020156"
}

Žugić, A., Tadić, V.,& Savić, S.. (2020). Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature. in Pharmaceutics
MDPI., 12(2).
https://doi.org/10.3390/pharmaceutics12020156

Žugić A, Tadić V, Savić S. Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature. in Pharmaceutics. 2020;12(2).
doi:10.3390/pharmaceutics12020156 .

Žugić, Ana, Tadić, Vanja, Savić, Snežana, "Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature" in Pharmaceutics, 12, no. 2 (2020),
https://doi.org/10.3390/pharmaceutics12020156 . .

TY  - JOUR
AU  - Žugić, Ana
AU  - Isaković, Aleksandra
AU  - Jeremić, Ivica
AU  - Savić, Snežana
AU  - Tadić, Vanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4664
AB  - Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.
PB  - Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line
VL  - 39
SP  - 30
EP  - 34
DO  - 10.5937/leksir1939030Z
ER  - 

@article{
author = "Žugić, Ana and Isaković, Aleksandra and Jeremić, Ivica and Savić, Snežana and Tadić, Vanja",
year = "2019",
abstract = "Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.",
publisher = "Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line",
volume = "39",
pages = "30-34",
doi = "10.5937/leksir1939030Z"
}

Žugić, A., Isaković, A., Jeremić, I., Savić, S.,& Tadić, V.. (2019). Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd., 39, 30-34.
https://doi.org/10.5937/leksir1939030Z

Žugić A, Isaković A, Jeremić I, Savić S, Tadić V. Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine. 2019;39:30-34.
doi:10.5937/leksir1939030Z .

Žugić, Ana, Isaković, Aleksandra, Jeremić, Ivica, Savić, Snežana, Tadić, Vanja, "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line" in Lekovite sirovine, 39 (2019):30-34,
https://doi.org/10.5937/leksir1939030Z . .

TY  - JOUR
AU  - Nesić, I.
AU  - Stojiljković, D.
AU  - Savić, Snežana
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3246
AB  - Objective The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non-ionic emulsifier-CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin. Methods Organoleptic and physico-chemical (pH values and electrical conductivity) analysis was performed, determination of fruit acids-FAs content (using HPLC analysis) and estimation of its antioxidant activity-AA (using DPPH test) during 180 days. In vivo study included following examinations: screening of safety profile (after creams application under occlusion during 24 h at human skin); skin moisturizing potential, transepidermal water loss-TEWL, skin pH after 28 days of cream application and hypopigmentation efficacy 7 days of cream application at artificially induced skin hyperpigmentation. Results Investigated cosmetic cream-CEW showed satisfactory organoleptic, physico-chemical characteristics, stability, FAs content (0.13%) and AA (19.25 +/- 0.67 %RSC) after preparation, which remained unchanged over the study period. In vivo investigation revealed absence of skin irritation after CEW's application under occlusion. An increase of skin moisturization (after 14 days Delta EC was 18.52 +/- 11.51 and after 28 days of applications 16.52 +/- 9.36) during 28 day-study, with unchanged TEWL and skin pH values was shown. Decrease of melanin index was revealed, too (after 7 days Delta MI was -31.40 +/- 16.50). Conclusion Cosmetic cream stabilized by conventional emulsifier showed better antioxidant potential and weaker moisturizing and hypopigmentation effects related to the cream with same composition but stabilized by biodegradable emulsifiers. Based on all mentioned above, investigated cosmetic cream might be considered for potential use as modern, stable, safe and efficient cosmetic product in the prevention and/or treatment of oxidative stress-related skin changes and/or damages, for moisturization of dry, even irritated skin as well as for lightening of hyperpigmented skin.
PB  - Wiley, Hoboken
T2  - International Journal of Cosmetic Science
T1  - Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers
VL  - 41
IS  - 3
SP  - 300
EP  - 310
DO  - 10.1111/ics.12537
ER  - 

@article{
author = "Nesić, I. and Stojiljković, D. and Savić, Snežana and Tasić-Kostov, Marija and Tadić, Vanja M.",
year = "2019",
abstract = "Objective The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non-ionic emulsifier-CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin. Methods Organoleptic and physico-chemical (pH values and electrical conductivity) analysis was performed, determination of fruit acids-FAs content (using HPLC analysis) and estimation of its antioxidant activity-AA (using DPPH test) during 180 days. In vivo study included following examinations: screening of safety profile (after creams application under occlusion during 24 h at human skin); skin moisturizing potential, transepidermal water loss-TEWL, skin pH after 28 days of cream application and hypopigmentation efficacy 7 days of cream application at artificially induced skin hyperpigmentation. Results Investigated cosmetic cream-CEW showed satisfactory organoleptic, physico-chemical characteristics, stability, FAs content (0.13%) and AA (19.25 +/- 0.67 %RSC) after preparation, which remained unchanged over the study period. In vivo investigation revealed absence of skin irritation after CEW's application under occlusion. An increase of skin moisturization (after 14 days Delta EC was 18.52 +/- 11.51 and after 28 days of applications 16.52 +/- 9.36) during 28 day-study, with unchanged TEWL and skin pH values was shown. Decrease of melanin index was revealed, too (after 7 days Delta MI was -31.40 +/- 16.50). Conclusion Cosmetic cream stabilized by conventional emulsifier showed better antioxidant potential and weaker moisturizing and hypopigmentation effects related to the cream with same composition but stabilized by biodegradable emulsifiers. Based on all mentioned above, investigated cosmetic cream might be considered for potential use as modern, stable, safe and efficient cosmetic product in the prevention and/or treatment of oxidative stress-related skin changes and/or damages, for moisturization of dry, even irritated skin as well as for lightening of hyperpigmented skin.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Cosmetic Science",
title = "Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers",
volume = "41",
number = "3",
pages = "300-310",
doi = "10.1111/ics.12537"
}

Nesić, I., Stojiljković, D., Savić, S., Tasić-Kostov, M.,& Tadić, V. M.. (2019). Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers. in International Journal of Cosmetic Science
Wiley, Hoboken., 41(3), 300-310.
https://doi.org/10.1111/ics.12537

Nesić I, Stojiljković D, Savić S, Tasić-Kostov M, Tadić VM. Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers. in International Journal of Cosmetic Science. 2019;41(3):300-310.
doi:10.1111/ics.12537 .

Nesić, I., Stojiljković, D., Savić, Snežana, Tasić-Kostov, Marija, Tadić, Vanja M., "Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers" in International Journal of Cosmetic Science, 41, no. 3 (2019):300-310,
https://doi.org/10.1111/ics.12537 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Tadić, Vanja M.
AU  - Kundaković, Tatjana
AU  - Savić, Snežana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3117
AB  - Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their great pharmacological potential lies in the fact that they represent specific symbiotic organisms and thus possess natural roles allowing them to be highly adaptable to different environmental conditions. On the other hand, stated biological activities of lichens with prospective medicinal significance may be connected to their long-term use in traditional treatment of various ailments. Genus Usnea from the Parmeliaceae family is certainly one of the best studied in terms of chemical composition and biological properties of its extracts and/or isolated compounds. In the first part of the study, a detailed review of literature has been performed yielding a detailed report on the investigations of biological activities of the lichens belonging to this genus. In the second part of the study, chemical composition of the lichens from the genus was described and, additionally, a survey of the biological properties of the most representative secondary metabolites in these lichens has been reported. It could be concluded that the extracts and/or isolated compounds from the lichens belonging to the genus Usnea may be considered a valuable source of prospective drug candidates with potential clinical relevance.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Chemical composition and biological activities of the extracts and secondary metabolites of lichens belonging to the genus Usnea, Parmeliaceae
IS  - 38
SP  - 68
EP  - 80
DO  - 10.5937/leksir1838068Z
ER  - 

@article{
author = "Žugić, Ana R. and Tadić, Vanja M. and Kundaković, Tatjana and Savić, Snežana",
year = "2018",
abstract = "Lichens represent a promising source of antimicrobial, cytotoxic and antioxidant agents. Their great pharmacological potential lies in the fact that they represent specific symbiotic organisms and thus possess natural roles allowing them to be highly adaptable to different environmental conditions. On the other hand, stated biological activities of lichens with prospective medicinal significance may be connected to their long-term use in traditional treatment of various ailments. Genus Usnea from the Parmeliaceae family is certainly one of the best studied in terms of chemical composition and biological properties of its extracts and/or isolated compounds. In the first part of the study, a detailed review of literature has been performed yielding a detailed report on the investigations of biological activities of the lichens belonging to this genus. In the second part of the study, chemical composition of the lichens from the genus was described and, additionally, a survey of the biological properties of the most representative secondary metabolites in these lichens has been reported. It could be concluded that the extracts and/or isolated compounds from the lichens belonging to the genus Usnea may be considered a valuable source of prospective drug candidates with potential clinical relevance.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Chemical composition and biological activities of the extracts and secondary metabolites of lichens belonging to the genus Usnea, Parmeliaceae",
number = "38",
pages = "68-80",
doi = "10.5937/leksir1838068Z"
}

Žugić, A. R., Tadić, V. M., Kundaković, T.,& Savić, S.. (2018). Chemical composition and biological activities of the extracts and secondary metabolites of lichens belonging to the genus Usnea, Parmeliaceae. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 68-80.
https://doi.org/10.5937/leksir1838068Z

Žugić AR, Tadić VM, Kundaković T, Savić S. Chemical composition and biological activities of the extracts and secondary metabolites of lichens belonging to the genus Usnea, Parmeliaceae. in Lekovite sirovine. 2018;(38):68-80.
doi:10.5937/leksir1838068Z .

Žugić, Ana R., Tadić, Vanja M., Kundaković, Tatjana, Savić, Snežana, "Chemical composition and biological activities of the extracts and secondary metabolites of lichens belonging to the genus Usnea, Parmeliaceae" in Lekovite sirovine, no. 38 (2018):68-80,
https://doi.org/10.5937/leksir1838068Z . .

TY  - JOUR
AU  - Nikolić, Ines
AU  - Lunter, Dominique
AU  - Ranđelović, Danijela
AU  - Žugić, Ana R.
AU  - Tadić, Vanja M.
AU  - Marković, Bojan
AU  - Cekić, Nebojša
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Potparević, Biljana
AU  - Daniels, Rolf
AU  - Savić, Snežana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3084
AB  - The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application
VL  - 550
IS  - 1-2
SP  - 333
EP  - 346
DO  - 10.1016/j.ijpharm.2018.08.060
ER  - 

@article{
author = "Nikolić, Ines and Lunter, Dominique and Ranđelović, Danijela and Žugić, Ana R. and Tadić, Vanja M. and Marković, Bojan and Cekić, Nebojša and Živković, Lada and Topalović, Dijana and Potparević, Biljana and Daniels, Rolf and Savić, Snežana",
year = "2018",
abstract = "The objective of this work was to investigate and profoundly characterize low-energy nanoemulsions as multifunctional carriers, with slight reference to dermal administration. An evidence-based approach was offered for deepening the knowledge on their formation via spontaneous emulsification. Curcumin, a compound of natural origin, potentially powerful therapeutic, was chosen as a model API. Due to curcumin's demanding properties (instability, poor solubility, low permeability), its potentials remain unreached. Low-energy nanoemulsions were considered carriers capable of overcoming imposed obstacles. Formulation consisting of Polysorbate 80 and soybean lecithin as stabilizers (9:1, 10%), medium-chain triglycerides as the oil phase (10%) and ultrapure water was selected for curcumin incorporation in 3 different concentrations (1, 2 and 3 mg/mL). Physicochemical stability was demonstrated during 3 months of monitoring (mean droplet size: 111.3-146.8 nm; PDI  lt  0.2; pH: 4.73-5.73). Curcumin's release from developed vehicles followed Higuchi's kinetics. DPPH (IC50 = 0.1187 mg/ mL) and FRAP (1.19 +/- 0.02 mmol/g) assays confirmed that curcumin acts as a potent antioxidant through different mechanisms, with no alterations after incorporation in the formulation. High biocompatibility in line with antigenotoxic activity of curcumin-loaded formulations (protective and reparative) was estimated through Comet assay. A multidisciplinary approach is needed to fully characterize developed systems, directing them to more concrete application possibilities.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application",
volume = "550",
number = "1-2",
pages = "333-346",
doi = "10.1016/j.ijpharm.2018.08.060"
}

Nikolić, I., Lunter, D., Ranđelović, D., Žugić, A. R., Tadić, V. M., Marković, B., Cekić, N., Živković, L., Topalović, D., Potparević, B., Daniels, R.,& Savić, S.. (2018). Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 550(1-2), 333-346.
https://doi.org/10.1016/j.ijpharm.2018.08.060

Nikolić I, Lunter D, Ranđelović D, Žugić AR, Tadić VM, Marković B, Cekić N, Živković L, Topalović D, Potparević B, Daniels R, Savić S. Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application. in International Journal of Pharmaceutics. 2018;550(1-2):333-346.
doi:10.1016/j.ijpharm.2018.08.060 .

Nikolić, Ines, Lunter, Dominique, Ranđelović, Danijela, Žugić, Ana R., Tadić, Vanja M., Marković, Bojan, Cekić, Nebojša, Živković, Lada, Topalović, Dijana, Potparević, Biljana, Daniels, Rolf, Savić, Snežana, "Curcumin-loaded low-energy nanoemulsions as a prototype of multifunctional vehicles for different administration routes: Physicochemical and in vitro peculiarities important for dermal application" in International Journal of Pharmaceutics, 550, no. 1-2 (2018):333-346,
https://doi.org/10.1016/j.ijpharm.2018.08.060 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lunter, Dominique
AU  - Daniels, Rolf
AU  - Pantelić, Ivana
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2588
AB  - Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Development and Technology
T1  - Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
VL  - 21
IS  - 5
SP  - 563
EP  - 575
DO  - 10.3109/10837450.2015.1026606
ER  - 

@article{
author = "Žugić, Ana R. and Lunter, Dominique and Daniels, Rolf and Pantelić, Ivana and Tasić-Kostov, Marija and Tadić, Vanja M. and Mišić, Dušan and Arsić, Ivana A. and Savić, Snežana",
year = "2016",
abstract = "Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Development and Technology",
title = "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product",
volume = "21",
number = "5",
pages = "563-575",
doi = "10.3109/10837450.2015.1026606"
}

Žugić, A. R., Lunter, D., Daniels, R., Pantelić, I., Tasić-Kostov, M., Tadić, V. M., Mišić, D., Arsić, I. A.,& Savić, S.. (2016). Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology
Taylor & Francis Ltd, Abingdon., 21(5), 563-575.
https://doi.org/10.3109/10837450.2015.1026606

Žugić AR, Lunter D, Daniels R, Pantelić I, Tasić-Kostov M, Tadić VM, Mišić D, Arsić IA, Savić S. Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology. 2016;21(5):563-575.
doi:10.3109/10837450.2015.1026606 .

Žugić, Ana R., Lunter, Dominique, Daniels, Rolf, Pantelić, Ivana, Tasić-Kostov, Marija, Tadić, Vanja M., Mišić, Dušan, Arsić, Ivana A., Savić, Snežana, "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product" in Pharmaceutical Development and Technology, 21, no. 5 (2016):563-575,
https://doi.org/10.3109/10837450.2015.1026606 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Jeremić, Ivica
AU  - Isaković, Aleksandra
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
AU  - Tadić, Vanja M.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2577
AB  - There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)
VL  - 11
IS  - 1
DO  - 10.1371/journal.pone.0146342
ER  - 

@article{
author = "Žugić, Ana R. and Jeremić, Ivica and Isaković, Aleksandra and Arsić, Ivana A. and Savić, Snežana and Tadić, Vanja M.",
year = "2016",
abstract = "There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)",
volume = "11",
number = "1",
doi = "10.1371/journal.pone.0146342"
}

Žugić, A. R., Jeremić, I., Isaković, A., Arsić, I. A., Savić, S.,& Tadić, V. M.. (2016). Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One
Public Library Science, San Francisco., 11(1).
https://doi.org/10.1371/journal.pone.0146342

Žugić AR, Jeremić I, Isaković A, Arsić IA, Savić S, Tadić VM. Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One. 2016;11(1).
doi:10.1371/journal.pone.0146342 .

Žugić, Ana R., Jeremić, Ivica, Isaković, Aleksandra, Arsić, Ivana A., Savić, Snežana, Tadić, Vanja M., "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)" in PLoS One, 11, no. 1 (2016),
https://doi.org/10.1371/journal.pone.0146342 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Arsić, Ivana A.
AU  - Mišić, Dušan
AU  - Petrović, Slobodan D.
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2477
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
VL  - 69
IS  - 6
SP  - 703
EP  - 712
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica and Tasić-Kostov, Marija and Tadić, Vanja M. and Arsić, Ivana A. and Mišić, Dušan and Petrović, Slobodan D. and Savić, Snežana",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
volume = "69",
number = "6",
pages = "703-712",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M., Tasić-Kostov, M., Tadić, V. M., Arsić, I. A., Mišić, D., Petrović, S. D.,& Savić, S.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić M, Tasić-Kostov M, Tadić VM, Arsić IA, Mišić D, Petrović SD, Savić S. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica, Tasić-Kostov, Marija, Tadić, Vanja M., Arsić, Ivana A., Mišić, Dušan, Petrović, Slobodan D., Savić, Snežana, "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Arsić, Ivana A.
AU  - Tadić, Vanja M.
AU  - Đorđević, Sofija
AU  - Žugić, Ana R.
AU  - Vujić, Zorica
AU  - Petrović, Slobodan D.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2267
AB  - Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99).
AB  - Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Optimization of extraction of antioxidant components from yarrow herb
T1  - Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave
VL  - 68
IS  - 4
SP  - 511
EP  - 517
DO  - 10.2298/HEMIND130910076A
ER  - 

@article{
author = "Arsić, Ivana A. and Tadić, Vanja M. and Đorđević, Sofija and Žugić, Ana R. and Vujić, Zorica and Petrović, Slobodan D.",
year = "2014",
abstract = "Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99)., Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Optimization of extraction of antioxidant components from yarrow herb, Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave",
volume = "68",
number = "4",
pages = "511-517",
doi = "10.2298/HEMIND130910076A"
}

Arsić, I. A., Tadić, V. M., Đorđević, S., Žugić, A. R., Vujić, Z.,& Petrović, S. D.. (2014). Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 68(4), 511-517.
https://doi.org/10.2298/HEMIND130910076A

Arsić IA, Tadić VM, Đorđević S, Žugić AR, Vujić Z, Petrović SD. Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija. 2014;68(4):511-517.
doi:10.2298/HEMIND130910076A .

Arsić, Ivana A., Tadić, Vanja M., Đorđević, Sofija, Žugić, Ana R., Vujić, Zorica, Petrović, Slobodan D., "Optimization of extraction of antioxidant components from yarrow herb" in Hemijska industrija, 68, no. 4 (2014):511-517,
https://doi.org/10.2298/HEMIND130910076A . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Tadić, Vanja M.
AU  - Petrović, Silvana
AU  - Kukić-Marković, Jelena
AU  - Dobrić, Silva
AU  - Milenković, Marina
AU  - Hadžifejzović, Nihad
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1688
AB  - The present study describes investigation on chemical profile and pharmacological properties of hydromethanolic extracts of Carlina acaulis and C. acanthifolia roots and herbs. In order to investigate the antioxidant properties several methods were applied: FRAP (Ferric Reducing Antioxidant Power) assay, TBA assay (Fe2+/ascorbic induced lipid peroxidation) and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, with indomethacin as a reference drug. Anti-ulcer activity of the extracts was investigated using ethanol-induced stress ulcer in rats and ranitidine as a reference drug. Antimicrobial activity was tested on five bacterial strains and one strain of fungi using the agar diffusion method. Investigated extracts exhibited considerable dose-dependent antioxidant, anti-inflammatory and anti-ulcer activities. Extracts of herbs were more active than the corresponding root extracts. All extracts exhibited moderate activity against all tested microorganisms. Herb extracts of C. acaulis and C. acanthifolia, as well as their root extracts exhibited similar bioactivity, which could be explained with their similar chemical composition revealed by HPLC analysis. The pharmacological activities of investigated Carlina extracts demonstrated here will serve as the basis for their further investigations in order to estimate their impact on human health and nutrition.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts
VL  - 7
IS  - 3
SP  - 1213
EP  - 1222
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1688
ER  - 

@article{
author = "Đorđević, Sofija and Tadić, Vanja M. and Petrović, Silvana and Kukić-Marković, Jelena and Dobrić, Silva and Milenković, Marina and Hadžifejzović, Nihad",
year = "2012",
abstract = "The present study describes investigation on chemical profile and pharmacological properties of hydromethanolic extracts of Carlina acaulis and C. acanthifolia roots and herbs. In order to investigate the antioxidant properties several methods were applied: FRAP (Ferric Reducing Antioxidant Power) assay, TBA assay (Fe2+/ascorbic induced lipid peroxidation) and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, with indomethacin as a reference drug. Anti-ulcer activity of the extracts was investigated using ethanol-induced stress ulcer in rats and ranitidine as a reference drug. Antimicrobial activity was tested on five bacterial strains and one strain of fungi using the agar diffusion method. Investigated extracts exhibited considerable dose-dependent antioxidant, anti-inflammatory and anti-ulcer activities. Extracts of herbs were more active than the corresponding root extracts. All extracts exhibited moderate activity against all tested microorganisms. Herb extracts of C. acaulis and C. acanthifolia, as well as their root extracts exhibited similar bioactivity, which could be explained with their similar chemical composition revealed by HPLC analysis. The pharmacological activities of investigated Carlina extracts demonstrated here will serve as the basis for their further investigations in order to estimate their impact on human health and nutrition.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts",
volume = "7",
number = "3",
pages = "1213-1222",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1688"
}

Đorđević, S., Tadić, V. M., Petrović, S., Kukić-Marković, J., Dobrić, S., Milenković, M.,& Hadžifejzović, N.. (2012). Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(3), 1213-1222.
https://hdl.handle.net/21.15107/rcub_farfar_1688

Đorđević S, Tadić VM, Petrović S, Kukić-Marković J, Dobrić S, Milenković M, Hadžifejzović N. Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts. in Digest Journal of Nanomaterials and Biostructures. 2012;7(3):1213-1222.
https://hdl.handle.net/21.15107/rcub_farfar_1688 .

Đorđević, Sofija, Tadić, Vanja M., Petrović, Silvana, Kukić-Marković, Jelena, Dobrić, Silva, Milenković, Marina, Hadžifejzović, Nihad, "Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts" in Digest Journal of Nanomaterials and Biostructures, 7, no. 3 (2012):1213-1222,
https://hdl.handle.net/21.15107/rcub_farfar_1688 .

TY  - JOUR
AU  - Cupara, Snežana
AU  - Šobajić, Slađana
AU  - Tadić, Vanja M.
AU  - Arsić, I
AU  - Đorđević, S.
AU  - Runjaić-Antić, Dušanka
AU  - Đorđević, Brižita
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1356
AB  - Sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae), is a thorny bush or small tree, native to Europe and Asia. Sea buckthorn fruit is rich in oil and carotenes which are found both in pericarp and seeds. Fruit and seed oils have significantly different fatty acid profiles and carotene levels. Previously reported data on the content of fatty acids and the carotenes in the oil are based on the fresh fruits. However, sea buckthorn fruits for traditional herbal use are available commonly in dry form. The goal of work was to analyze the content of fatty acids and carotenes in the fruit pericarp oil and seed oil obtained from dry sea buckthorn fruits. Physical and Chemical constants of the oils were also determined. It was found that the oil yield from pericarp and seeds were 18,8 and 7,4 respectively (expressed in g of oil per 100 g of dry plant material). The composition of fatty acids was determined by GC in the methyl ester form. The main fatty acids in pericarp oil were palmitic, palmitoleic, and oleic acids (35,2 %, 28,5 %, and 29,9 % respectively). Seed oil was, on the other hand, rich in oleic, linoleic and alpha-linolenic acids (23,7 %, 37,6 %, 20,5 % respectively). The content of carotenes in oil obtained from fresh sources varies extremely within different subspecies and it is influenced strongly by methods of oil isolation. The common reported levels of carotenes in pericarp and seed oil obtained from fresh fruits are 100-500mg/100g and 20-100 mg/100 g respectively. Our findings have significantly lower values then the average reported data, which points that dehydration of sea buckthorn berries influences negatively upon the content of carotenes, and then fatty oil content remains roughly the same as in fresh fruits. From medical point of view and dried fruits are a valuable source of polyunsaturated fatty acids and monounsaturated you have a favorable effect primarily on the cardiovascular system.
PB  - Drunpp-Sarajevo, Sarajevo
T2  - HealthMed
T1  - Dry sea buckthorn berries (Hippophae rhamnoides L.) - fatty acid and carotene content in pericarp and seed oil
VL  - 4
IS  - 4
SP  - 788
EP  - 791
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1356
ER  - 

@article{
author = "Cupara, Snežana and Šobajić, Slađana and Tadić, Vanja M. and Arsić, I and Đorđević, S. and Runjaić-Antić, Dušanka and Đorđević, Brižita",
year = "2010",
abstract = "Sea buckthorn (Hippophae rhamnoides L., Elaeagnaceae), is a thorny bush or small tree, native to Europe and Asia. Sea buckthorn fruit is rich in oil and carotenes which are found both in pericarp and seeds. Fruit and seed oils have significantly different fatty acid profiles and carotene levels. Previously reported data on the content of fatty acids and the carotenes in the oil are based on the fresh fruits. However, sea buckthorn fruits for traditional herbal use are available commonly in dry form. The goal of work was to analyze the content of fatty acids and carotenes in the fruit pericarp oil and seed oil obtained from dry sea buckthorn fruits. Physical and Chemical constants of the oils were also determined. It was found that the oil yield from pericarp and seeds were 18,8 and 7,4 respectively (expressed in g of oil per 100 g of dry plant material). The composition of fatty acids was determined by GC in the methyl ester form. The main fatty acids in pericarp oil were palmitic, palmitoleic, and oleic acids (35,2 %, 28,5 %, and 29,9 % respectively). Seed oil was, on the other hand, rich in oleic, linoleic and alpha-linolenic acids (23,7 %, 37,6 %, 20,5 % respectively). The content of carotenes in oil obtained from fresh sources varies extremely within different subspecies and it is influenced strongly by methods of oil isolation. The common reported levels of carotenes in pericarp and seed oil obtained from fresh fruits are 100-500mg/100g and 20-100 mg/100 g respectively. Our findings have significantly lower values then the average reported data, which points that dehydration of sea buckthorn berries influences negatively upon the content of carotenes, and then fatty oil content remains roughly the same as in fresh fruits. From medical point of view and dried fruits are a valuable source of polyunsaturated fatty acids and monounsaturated you have a favorable effect primarily on the cardiovascular system.",
publisher = "Drunpp-Sarajevo, Sarajevo",
journal = "HealthMed",
title = "Dry sea buckthorn berries (Hippophae rhamnoides L.) - fatty acid and carotene content in pericarp and seed oil",
volume = "4",
number = "4",
pages = "788-791",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1356"
}

Cupara, S., Šobajić, S., Tadić, V. M., Arsić, I., Đorđević, S., Runjaić-Antić, D.,& Đorđević, B.. (2010). Dry sea buckthorn berries (Hippophae rhamnoides L.) - fatty acid and carotene content in pericarp and seed oil. in HealthMed
Drunpp-Sarajevo, Sarajevo., 4(4), 788-791.
https://hdl.handle.net/21.15107/rcub_farfar_1356

Cupara S, Šobajić S, Tadić VM, Arsić I, Đorđević S, Runjaić-Antić D, Đorđević B. Dry sea buckthorn berries (Hippophae rhamnoides L.) - fatty acid and carotene content in pericarp and seed oil. in HealthMed. 2010;4(4):788-791.
https://hdl.handle.net/21.15107/rcub_farfar_1356 .

Cupara, Snežana, Šobajić, Slađana, Tadić, Vanja M., Arsić, I, Đorđević, S., Runjaić-Antić, Dušanka, Đorđević, Brižita, "Dry sea buckthorn berries (Hippophae rhamnoides L.) - fatty acid and carotene content in pericarp and seed oil" in HealthMed, 4, no. 4 (2010):788-791,
https://hdl.handle.net/21.15107/rcub_farfar_1356 .

TY  - CONF
AU  - Tadić, Vanja M.
AU  - Đorđević, S.
AU  - Arsić, I
AU  - Nikolić, Katarina
AU  - Gligorijević, Nevenka
AU  - Radulović, Siniša
AU  - Marković, G.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1093
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Cytotoxic activity and antioxidative properties of Sideritis scardica extracts
VL  - 74
IS  - 9
SP  - 990
EP  - 990
DO  - 10.1055/s-0028-1084204
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1093
ER  - 

@conference{
author = "Tadić, Vanja M. and Đorđević, S. and Arsić, I and Nikolić, Katarina and Gligorijević, Nevenka and Radulović, Siniša and Marković, G.",
year = "2008",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Cytotoxic activity and antioxidative properties of Sideritis scardica extracts",
volume = "74",
number = "9",
pages = "990-990",
doi = "10.1055/s-0028-1084204",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1093"
}

Tadić, V. M., Đorđević, S., Arsić, I., Nikolić, K., Gligorijević, N., Radulović, S.,& Marković, G.. (2008). Cytotoxic activity and antioxidative properties of Sideritis scardica extracts. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 74(9), 990-990.
https://doi.org/10.1055/s-0028-1084204
https://hdl.handle.net/21.15107/rcub_farfar_1093

Tadić VM, Đorđević S, Arsić I, Nikolić K, Gligorijević N, Radulović S, Marković G. Cytotoxic activity and antioxidative properties of Sideritis scardica extracts. in Planta Medica. 2008;74(9):990-990.
doi:10.1055/s-0028-1084204
https://hdl.handle.net/21.15107/rcub_farfar_1093 .

Tadić, Vanja M., Đorđević, S., Arsić, I, Nikolić, Katarina, Gligorijević, Nevenka, Radulović, Siniša, Marković, G., "Cytotoxic activity and antioxidative properties of Sideritis scardica extracts" in Planta Medica, 74, no. 9 (2008):990-990,
https://doi.org/10.1055/s-0028-1084204 .,
https://hdl.handle.net/21.15107/rcub_farfar_1093 .

TY  - CONF
AU  - Đorđević, Sofija
AU  - Arsić, I.
AU  - Petrović, Silvana
AU  - Tadić, Vanja M.
AU  - Milenković, Marina
AU  - Isailović, G.
AU  - Adamović, A.
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1113
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - In vivo investigation on antifungal activity of cream with Carlina acanthifolia essential oil
VL  - 74
IS  - 9
SP  - 1193
EP  - 1193
DO  - 10.1055/s-0028-1084926
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1113
ER  - 

@conference{
author = "Đorđević, Sofija and Arsić, I. and Petrović, Silvana and Tadić, Vanja M. and Milenković, Marina and Isailović, G. and Adamović, A.",
year = "2008",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "In vivo investigation on antifungal activity of cream with Carlina acanthifolia essential oil",
volume = "74",
number = "9",
pages = "1193-1193",
doi = "10.1055/s-0028-1084926",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1113"
}

Đorđević, S., Arsić, I., Petrović, S., Tadić, V. M., Milenković, M., Isailović, G.,& Adamović, A.. (2008). In vivo investigation on antifungal activity of cream with Carlina acanthifolia essential oil. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 74(9), 1193-1193.
https://doi.org/10.1055/s-0028-1084926
https://hdl.handle.net/21.15107/rcub_farfar_1113

Đorđević S, Arsić I, Petrović S, Tadić VM, Milenković M, Isailović G, Adamović A. In vivo investigation on antifungal activity of cream with Carlina acanthifolia essential oil. in Planta Medica. 2008;74(9):1193-1193.
doi:10.1055/s-0028-1084926
https://hdl.handle.net/21.15107/rcub_farfar_1113 .

Đorđević, Sofija, Arsić, I., Petrović, Silvana, Tadić, Vanja M., Milenković, Marina, Isailović, G., Adamović, A., "In vivo investigation on antifungal activity of cream with Carlina acanthifolia essential oil" in Planta Medica, 74, no. 9 (2008):1193-1193,
https://doi.org/10.1055/s-0028-1084926 .,
https://hdl.handle.net/21.15107/rcub_farfar_1113 .

TY  - CONF
AU  - Tadić, Vanja M.
AU  - Đorđević, S.
AU  - Arsić, I
AU  - Dobrić, Silva
AU  - Milenković, Marina
AU  - Antić-Stanković, Jelena
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/977
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Anti-inflammatory and antimicrobial activity of Sideritis scardica extracts
VL  - 73
IS  - 9
SP  - 854
EP  - 854
DO  - 10.1055/s-2007-986880
UR  - https://hdl.handle.net/21.15107/rcub_farfar_977
ER  - 

@conference{
author = "Tadić, Vanja M. and Đorđević, S. and Arsić, I and Dobrić, Silva and Milenković, Marina and Antić-Stanković, Jelena",
year = "2007",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Anti-inflammatory and antimicrobial activity of Sideritis scardica extracts",
volume = "73",
number = "9",
pages = "854-854",
doi = "10.1055/s-2007-986880",
url = "https://hdl.handle.net/21.15107/rcub_farfar_977"
}

Tadić, V. M., Đorđević, S., Arsić, I., Dobrić, S., Milenković, M.,& Antić-Stanković, J.. (2007). Anti-inflammatory and antimicrobial activity of Sideritis scardica extracts. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 73(9), 854-854.
https://doi.org/10.1055/s-2007-986880
https://hdl.handle.net/21.15107/rcub_farfar_977

Tadić VM, Đorđević S, Arsić I, Dobrić S, Milenković M, Antić-Stanković J. Anti-inflammatory and antimicrobial activity of Sideritis scardica extracts. in Planta Medica. 2007;73(9):854-854.
doi:10.1055/s-2007-986880
https://hdl.handle.net/21.15107/rcub_farfar_977 .

Tadić, Vanja M., Đorđević, S., Arsić, I, Dobrić, Silva, Milenković, Marina, Antić-Stanković, Jelena, "Anti-inflammatory and antimicrobial activity of Sideritis scardica extracts" in Planta Medica, 73, no. 9 (2007):854-854,
https://doi.org/10.1055/s-2007-986880 .,
https://hdl.handle.net/21.15107/rcub_farfar_977 .

TY  - CONF
AU  - Đorđević, S.
AU  - Tadić, Vanja M.
AU  - Arsić, I.
AU  - Dobrić, Silva
AU  - Milenković, Marina
AU  - Stević, T.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/772
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Characterization of Sideritis scardica Griseb. extracts
T1  - Karakterizacija ekstrakta šarplaninskog čaja
VL  - 56
IS  - 5
SP  - 912
EP  - 913
UR  - https://hdl.handle.net/21.15107/rcub_farfar_772
ER  - 

@conference{
author = "Đorđević, S. and Tadić, Vanja M. and Arsić, I. and Dobrić, Silva and Milenković, Marina and Stević, T.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Characterization of Sideritis scardica Griseb. extracts, Karakterizacija ekstrakta šarplaninskog čaja",
volume = "56",
number = "5",
pages = "912-913",
url = "https://hdl.handle.net/21.15107/rcub_farfar_772"
}

Đorđević, S., Tadić, V. M., Arsić, I., Dobrić, S., Milenković, M.,& Stević, T.. (2006). Characterization of Sideritis scardica Griseb. extracts. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 912-913.
https://hdl.handle.net/21.15107/rcub_farfar_772

Đorđević S, Tadić VM, Arsić I, Dobrić S, Milenković M, Stević T. Characterization of Sideritis scardica Griseb. extracts. in Arhiv za farmaciju. 2006;56(5):912-913.
https://hdl.handle.net/21.15107/rcub_farfar_772 .

Đorđević, S., Tadić, Vanja M., Arsić, I., Dobrić, Silva, Milenković, Marina, Stević, T., "Characterization of Sideritis scardica Griseb. extracts" in Arhiv za farmaciju, 56, no. 5 (2006):912-913,
https://hdl.handle.net/21.15107/rcub_farfar_772 .