TY  - JOUR
AU  - Vajdle, O
AU  - Guzsvány, V
AU  - Škorić, D
AU  - Csanádi, J
AU  - Petković, Miloš
AU  - Avramov-Ivić, M
AU  - Kónya, Z
AU  - Petrović, Slobodan D.
AU  - Bobrowski, A
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2970
AB  - The renewable silver-amalgam film electrode (Hg(Ag)FE) was applied for voltammetric characterization and determination of semi-synthetic macrolide antibiotics azithromycin (AZI), clarithromycin (CLA) and roxithromycin (ROX) in the Britton-Robinson buffer as supporting electrolyte ranging the pH from 4.0 to 11.9. All three macrolides showed reduction signals in fairly negative potential range. During direct cathodic square wave voltammetric (SWV) investigations conducted over the potential range from −0.75 V to −2.00 V vs SCE, either one or two reduction peaks were obtained in the potential range from −1.5 to −1.9 V. The shapes and intensities of the signals depend on the applied pH values in wider pH ranges. For analytical purposes concerning the development of direct cathodic SWV and adsorptive stripping SWV (SW-AdSV) methods the neutral and slightly alkaline media were suitable as pH 7.2, pH 7.4 and pH 7.0 for AZI, CLA and ROX, respectively. Based on the cyclic voltammograms recorded at these pH values, adsorption-controlled electrode kinetics process can be proposed for all three macrolides. Furthermore, the water suppressed 1H NMR measurements in the pH range between 6.0 and 10.5 indicated that the macrolide molecules at the optimal analytical conditions are predominantly in protonated form via their tertiary amino groups which supported in all three cases their adsorption on the appropriately polarized Hg(Ag)FE electrode. The optimized direct cathodic SWV methods showed good linearity in concentration ranges 4.81–23.3 μg mL−1, 1.96–28.6 μg mL−1 and 1.48–25.9 μg mL−1 for AZI, CLA and ROX, respectively. The development of the SW-AdSV methods resulted in the linear responses at lower concentration ranges as 1.0–2.46 μg mL−1, 0.05–0.99 μg mL−1 and 0.10–0.99 μg mL−1, for AZI, CLA and ROX, respectively. The relative standard deviation for all developed methods was not higher than 1.0% except the SWV method for AZI with 4.7%. In the case of all three investigated macrolide antibiotics the protonated form of the tertiary amino group(s) at appropriate accumulation potential and time favored the adsorption of the ionic form of the target molecules offering the opportunity for the development of SW-AdSV methods for their trace level analysis on Hg(Ag)FE. Optimized SW-AdSV method was applied for determination of ROX in pharmaceutical preparation Runac®.
PB  - Elsevier Ltd
T2  - Electrochimica Acta
T1  - Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode
VL  - 229
SP  - 334
EP  - 344
DO  - 10.1016/j.electacta.2017.01.146
ER  - 

@article{
author = "Vajdle, O and Guzsvány, V and Škorić, D and Csanádi, J and Petković, Miloš and Avramov-Ivić, M and Kónya, Z and Petrović, Slobodan D. and Bobrowski, A",
year = "2017",
abstract = "The renewable silver-amalgam film electrode (Hg(Ag)FE) was applied for voltammetric characterization and determination of semi-synthetic macrolide antibiotics azithromycin (AZI), clarithromycin (CLA) and roxithromycin (ROX) in the Britton-Robinson buffer as supporting electrolyte ranging the pH from 4.0 to 11.9. All three macrolides showed reduction signals in fairly negative potential range. During direct cathodic square wave voltammetric (SWV) investigations conducted over the potential range from −0.75 V to −2.00 V vs SCE, either one or two reduction peaks were obtained in the potential range from −1.5 to −1.9 V. The shapes and intensities of the signals depend on the applied pH values in wider pH ranges. For analytical purposes concerning the development of direct cathodic SWV and adsorptive stripping SWV (SW-AdSV) methods the neutral and slightly alkaline media were suitable as pH 7.2, pH 7.4 and pH 7.0 for AZI, CLA and ROX, respectively. Based on the cyclic voltammograms recorded at these pH values, adsorption-controlled electrode kinetics process can be proposed for all three macrolides. Furthermore, the water suppressed 1H NMR measurements in the pH range between 6.0 and 10.5 indicated that the macrolide molecules at the optimal analytical conditions are predominantly in protonated form via their tertiary amino groups which supported in all three cases their adsorption on the appropriately polarized Hg(Ag)FE electrode. The optimized direct cathodic SWV methods showed good linearity in concentration ranges 4.81–23.3 μg mL−1, 1.96–28.6 μg mL−1 and 1.48–25.9 μg mL−1 for AZI, CLA and ROX, respectively. The development of the SW-AdSV methods resulted in the linear responses at lower concentration ranges as 1.0–2.46 μg mL−1, 0.05–0.99 μg mL−1 and 0.10–0.99 μg mL−1, for AZI, CLA and ROX, respectively. The relative standard deviation for all developed methods was not higher than 1.0% except the SWV method for AZI with 4.7%. In the case of all three investigated macrolide antibiotics the protonated form of the tertiary amino group(s) at appropriate accumulation potential and time favored the adsorption of the ionic form of the target molecules offering the opportunity for the development of SW-AdSV methods for their trace level analysis on Hg(Ag)FE. Optimized SW-AdSV method was applied for determination of ROX in pharmaceutical preparation Runac®.",
publisher = "Elsevier Ltd",
journal = "Electrochimica Acta",
title = "Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode",
volume = "229",
pages = "334-344",
doi = "10.1016/j.electacta.2017.01.146"
}

Vajdle, O., Guzsvány, V., Škorić, D., Csanádi, J., Petković, M., Avramov-Ivić, M., Kónya, Z., Petrović, S. D.,& Bobrowski, A.. (2017). Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode. in Electrochimica Acta
Elsevier Ltd., 229, 334-344.
https://doi.org/10.1016/j.electacta.2017.01.146

Vajdle O, Guzsvány V, Škorić D, Csanádi J, Petković M, Avramov-Ivić M, Kónya Z, Petrović SD, Bobrowski A. Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode. in Electrochimica Acta. 2017;229:334-344.
doi:10.1016/j.electacta.2017.01.146 .

Vajdle, O, Guzsvány, V, Škorić, D, Csanádi, J, Petković, Miloš, Avramov-Ivić, M, Kónya, Z, Petrović, Slobodan D., Bobrowski, A, "Voltammetric behavior and determination of the macrolide antibiotics azithromycin, clarithromycin and roxithromycin at a renewable silver – amalgam film electrode" in Electrochimica Acta, 229 (2017):334-344,
https://doi.org/10.1016/j.electacta.2017.01.146 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Arsić, Ivana A.
AU  - Mišić, Dušan
AU  - Petrović, Slobodan D.
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2477
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
VL  - 69
IS  - 6
SP  - 703
EP  - 712
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica and Tasić-Kostov, Marija and Tadić, Vanja M. and Arsić, Ivana A. and Mišić, Dušan and Petrović, Slobodan D. and Savić, Snežana",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
volume = "69",
number = "6",
pages = "703-712",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M., Tasić-Kostov, M., Tadić, V. M., Arsić, I. A., Mišić, D., Petrović, S. D.,& Savić, S.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić M, Tasić-Kostov M, Tadić VM, Arsić IA, Mišić D, Petrović SD, Savić S. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica, Tasić-Kostov, Marija, Tadić, Vanja M., Arsić, Ivana A., Mišić, Dušan, Petrović, Slobodan D., Savić, Snežana, "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Arsić, Ivana A.
AU  - Tadić, Vanja M.
AU  - Đorđević, Sofija
AU  - Žugić, Ana R.
AU  - Vujić, Zorica
AU  - Petrović, Slobodan D.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2267
AB  - Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99).
AB  - Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Optimization of extraction of antioxidant components from yarrow herb
T1  - Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave
VL  - 68
IS  - 4
SP  - 511
EP  - 517
DO  - 10.2298/HEMIND130910076A
ER  - 

@article{
author = "Arsić, Ivana A. and Tadić, Vanja M. and Đorđević, Sofija and Žugić, Ana R. and Vujić, Zorica and Petrović, Slobodan D.",
year = "2014",
abstract = "Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99)., Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Optimization of extraction of antioxidant components from yarrow herb, Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave",
volume = "68",
number = "4",
pages = "511-517",
doi = "10.2298/HEMIND130910076A"
}

Arsić, I. A., Tadić, V. M., Đorđević, S., Žugić, A. R., Vujić, Z.,& Petrović, S. D.. (2014). Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 68(4), 511-517.
https://doi.org/10.2298/HEMIND130910076A

Arsić IA, Tadić VM, Đorđević S, Žugić AR, Vujić Z, Petrović SD. Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija. 2014;68(4):511-517.
doi:10.2298/HEMIND130910076A .

Arsić, Ivana A., Tadić, Vanja M., Đorđević, Sofija, Žugić, Ana R., Vujić, Zorica, Petrović, Slobodan D., "Optimization of extraction of antioxidant components from yarrow herb" in Hemijska industrija, 68, no. 4 (2014):511-517,
https://doi.org/10.2298/HEMIND130910076A . .

TY  - JOUR
AU  - Zizović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukić-Marković, Jelena
AU  - Petrović, Slobodan D.
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1665
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
VL  - 72
SP  - 7
EP  - 14
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Zizović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukić-Marković, Jelena and Petrović, Slobodan D.",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
volume = "72",
pages = "7-14",
doi = "10.1016/j.supflu.2012.07.018"
}

Zizović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukić-Marković, J.,& Petrović, S. D.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science BV, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Zizović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukić-Marković J, Petrović SD. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Zizović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukić-Marković, Jelena, Petrović, Slobodan D., "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Ibrić, Svetlana
AU  - Jovanović, M
AU  - Đurić, Zorica
AU  - Parojčić, Jelena
AU  - Petrović, Slobodan D.
AU  - Solomun, Ljiljana
AU  - Stupar, Biljana
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/469
AB  - The purpose of the present study was to model the effects of the concentration of Eudragit L 100 and compression pressure as the most important process and formulation variables on the in vitro release profile of aspirin from matrix tablets formulated with Eudragit L 100 as matrix substance and to optimize the formulation by artificial neural network. As model formulations, 10 kinds of aspirin matrix tablets were prepared. The amount of Eudragit L 100 and the compression pressure were selected as causal factors. In vitro dissolution time profiles at 4 different sampling times were chosen as responses. A set of release parameters and causal factors were used as tutorial data for the generalized regression neural network (GRNN) and analyzed using a computer. Observed results of drug release studies indicate that drug release rates vary widely between investigated formulations, with a range of 5 hours to more than 10 hours to complete dissolution. The GRNN model was optimized. The root mean square value for the trained network was 1.12%, which indicated that the optimal GRNN model was reached. Applying the generalized distance function method, the optimal tablet formulation predicted by GRNN was with 5% of Eudragit L 100 and tablet hardness 60N. Calculated difference (f1 2.465) and similarity (f2 85.61) factors indicate that there is no difference between predicted and experimentally observed drug release profiles for the optimal formulation. This work illustrates the potential for an artificial neural network, GRNN, to assist in development of extended release dosage forms.
PB  - AAPS PharmSci Editorial Office
T2  - AAPS PharmSciTech
T1  - Artificial neural networks in the modeling and optimization of aspirin extended release tablets with Eudragit L 100 as matrix substance
VL  - 4
IS  - 1
DO  - 10.1208/pt040109
ER  - 

@article{
author = "Ibrić, Svetlana and Jovanović, M and Đurić, Zorica and Parojčić, Jelena and Petrović, Slobodan D. and Solomun, Ljiljana and Stupar, Biljana",
year = "2003",
abstract = "The purpose of the present study was to model the effects of the concentration of Eudragit L 100 and compression pressure as the most important process and formulation variables on the in vitro release profile of aspirin from matrix tablets formulated with Eudragit L 100 as matrix substance and to optimize the formulation by artificial neural network. As model formulations, 10 kinds of aspirin matrix tablets were prepared. The amount of Eudragit L 100 and the compression pressure were selected as causal factors. In vitro dissolution time profiles at 4 different sampling times were chosen as responses. A set of release parameters and causal factors were used as tutorial data for the generalized regression neural network (GRNN) and analyzed using a computer. Observed results of drug release studies indicate that drug release rates vary widely between investigated formulations, with a range of 5 hours to more than 10 hours to complete dissolution. The GRNN model was optimized. The root mean square value for the trained network was 1.12%, which indicated that the optimal GRNN model was reached. Applying the generalized distance function method, the optimal tablet formulation predicted by GRNN was with 5% of Eudragit L 100 and tablet hardness 60N. Calculated difference (f1 2.465) and similarity (f2 85.61) factors indicate that there is no difference between predicted and experimentally observed drug release profiles for the optimal formulation. This work illustrates the potential for an artificial neural network, GRNN, to assist in development of extended release dosage forms.",
publisher = "AAPS PharmSci Editorial Office",
journal = "AAPS PharmSciTech",
title = "Artificial neural networks in the modeling and optimization of aspirin extended release tablets with Eudragit L 100 as matrix substance",
volume = "4",
number = "1",
doi = "10.1208/pt040109"
}

Ibrić, S., Jovanović, M., Đurić, Z., Parojčić, J., Petrović, S. D., Solomun, L.,& Stupar, B.. (2003). Artificial neural networks in the modeling and optimization of aspirin extended release tablets with Eudragit L 100 as matrix substance. in AAPS PharmSciTech
AAPS PharmSci Editorial Office., 4(1).
https://doi.org/10.1208/pt040109

Ibrić S, Jovanović M, Đurić Z, Parojčić J, Petrović SD, Solomun L, Stupar B. Artificial neural networks in the modeling and optimization of aspirin extended release tablets with Eudragit L 100 as matrix substance. in AAPS PharmSciTech. 2003;4(1).
doi:10.1208/pt040109 .

Ibrić, Svetlana, Jovanović, M, Đurić, Zorica, Parojčić, Jelena, Petrović, Slobodan D., Solomun, Ljiljana, Stupar, Biljana, "Artificial neural networks in the modeling and optimization of aspirin extended release tablets with Eudragit L 100 as matrix substance" in AAPS PharmSciTech, 4, no. 1 (2003),
https://doi.org/10.1208/pt040109 . .