TY  - JOUR
AU  - Žugić, Ana
AU  - Isaković, Aleksandra
AU  - Jeremić, Ivica
AU  - Savić, Snežana
AU  - Tadić, Vanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4664
AB  - Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.
PB  - Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line
VL  - 39
SP  - 30
EP  - 34
DO  - 10.5937/leksir1939030Z
ER  - 

@article{
author = "Žugić, Ana and Isaković, Aleksandra and Jeremić, Ivica and Savić, Snežana and Tadić, Vanja",
year = "2019",
abstract = "Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.",
publisher = "Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line",
volume = "39",
pages = "30-34",
doi = "10.5937/leksir1939030Z"
}

Žugić, A., Isaković, A., Jeremić, I., Savić, S.,& Tadić, V.. (2019). Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd., 39, 30-34.
https://doi.org/10.5937/leksir1939030Z

Žugić A, Isaković A, Jeremić I, Savić S, Tadić V. Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine. 2019;39:30-34.
doi:10.5937/leksir1939030Z .

Žugić, Ana, Isaković, Aleksandra, Jeremić, Ivica, Savić, Snežana, Tadić, Vanja, "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line" in Lekovite sirovine, 39 (2019):30-34,
https://doi.org/10.5937/leksir1939030Z . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Jeremić, Ivica
AU  - Isaković, Aleksandra
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
AU  - Tadić, Vanja M.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2577
AB  - There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)
VL  - 11
IS  - 1
DO  - 10.1371/journal.pone.0146342
ER  - 

@article{
author = "Žugić, Ana R. and Jeremić, Ivica and Isaković, Aleksandra and Arsić, Ivana A. and Savić, Snežana and Tadić, Vanja M.",
year = "2016",
abstract = "There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)",
volume = "11",
number = "1",
doi = "10.1371/journal.pone.0146342"
}

Žugić, A. R., Jeremić, I., Isaković, A., Arsić, I. A., Savić, S.,& Tadić, V. M.. (2016). Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One
Public Library Science, San Francisco., 11(1).
https://doi.org/10.1371/journal.pone.0146342

Žugić AR, Jeremić I, Isaković A, Arsić IA, Savić S, Tadić VM. Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One. 2016;11(1).
doi:10.1371/journal.pone.0146342 .

Žugić, Ana R., Jeremić, Ivica, Isaković, Aleksandra, Arsić, Ivana A., Savić, Snežana, Tadić, Vanja M., "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)" in PLoS One, 11, no. 1 (2016),
https://doi.org/10.1371/journal.pone.0146342 . .