TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Stanišić, Verica
AU  - Milović, Sanja
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ninić, Ana
AU  - Kotur-Stevuljević, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3601
AB  - Introduction: We aimed to examine serum endocan level and the summary involvement of dyslipidemia, oxidative stress (OS) and inflammation by calculation of its comprehensive score (i.e. Dyslipidemia-Oxy-Inflammation (DOI) score) in relation to glucoregulation in subjects with prediabetes and overt type 2 diabetes (T2D). Material and methods: A total of 59 patients with prediabetes and 102 patients with T2D were compared with 117 diabetes-free controls. Glycated hemoglobin (HbA1c), inflammation, OS and lipid parameters were measured. Associations of clinical data with HbA1c level were tested with univariate and multivariate logistic ordinal regression analysis. HbA1c as a dependent variable is given at the ordinal level (i.e. < 5.7%; 5.7-6.4%, > 6.4%, respectively). Results: Endocan was significantly higher in the T2D group than in the controls. As endocan concentration rose by 1 unit, the probability for higher HbA1c concentration increased by more than 3 times (OR = 3.69, 95% CI: 1.84-7.01, p < 0.001). Also, a rise in the dyslipidemia score, oxy score, inflammation score and DOI score by 1 unit increased the probability of higher HbA1c concentration by 19%, 13%, 51% and 11%, respectively. In the models, after adjustment for confounding variables, endocan and DOI score remained independent predictors of HbA1c level. Conclusions: Endocan and DOI score are independently correlated with HbA1c in patients with prediabetes and overt T2D.
PB  - Termedia Publishing House Ltd.
T2  - Archives of Medical Science
T1  - Endocan and a novel score for dyslipidemia, oxidative stress and inflammation (DOI score) are independently correlated with glycated hemoglobin (HbA1c) in patients with prediabetes and type 2 diabetes
VL  - 16
IS  - 1
SP  - 42
EP  - 50
DO  - 10.5114/aoms.2019.87541
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Stanišić, Verica and Milović, Sanja and Spasojević-Kalimanovska, Vesna and Ninić, Ana and Kotur-Stevuljević, Jelena",
year = "2020",
abstract = "Introduction: We aimed to examine serum endocan level and the summary involvement of dyslipidemia, oxidative stress (OS) and inflammation by calculation of its comprehensive score (i.e. Dyslipidemia-Oxy-Inflammation (DOI) score) in relation to glucoregulation in subjects with prediabetes and overt type 2 diabetes (T2D). Material and methods: A total of 59 patients with prediabetes and 102 patients with T2D were compared with 117 diabetes-free controls. Glycated hemoglobin (HbA1c), inflammation, OS and lipid parameters were measured. Associations of clinical data with HbA1c level were tested with univariate and multivariate logistic ordinal regression analysis. HbA1c as a dependent variable is given at the ordinal level (i.e. < 5.7%; 5.7-6.4%, > 6.4%, respectively). Results: Endocan was significantly higher in the T2D group than in the controls. As endocan concentration rose by 1 unit, the probability for higher HbA1c concentration increased by more than 3 times (OR = 3.69, 95% CI: 1.84-7.01, p < 0.001). Also, a rise in the dyslipidemia score, oxy score, inflammation score and DOI score by 1 unit increased the probability of higher HbA1c concentration by 19%, 13%, 51% and 11%, respectively. In the models, after adjustment for confounding variables, endocan and DOI score remained independent predictors of HbA1c level. Conclusions: Endocan and DOI score are independently correlated with HbA1c in patients with prediabetes and overt T2D.",
publisher = "Termedia Publishing House Ltd.",
journal = "Archives of Medical Science",
title = "Endocan and a novel score for dyslipidemia, oxidative stress and inflammation (DOI score) are independently correlated with glycated hemoglobin (HbA1c) in patients with prediabetes and type 2 diabetes",
volume = "16",
number = "1",
pages = "42-50",
doi = "10.5114/aoms.2019.87541"
}

Klisić, A., Kavarić, N., Stanišić, V., Milović, S., Spasojević-Kalimanovska, V., Ninić, A.,& Kotur-Stevuljević, J.. (2020). Endocan and a novel score for dyslipidemia, oxidative stress and inflammation (DOI score) are independently correlated with glycated hemoglobin (HbA1c) in patients with prediabetes and type 2 diabetes. in Archives of Medical Science
Termedia Publishing House Ltd.., 16(1), 42-50.
https://doi.org/10.5114/aoms.2019.87541

Klisić A, Kavarić N, Stanišić V, Milović S, Spasojević-Kalimanovska V, Ninić A, Kotur-Stevuljević J. Endocan and a novel score for dyslipidemia, oxidative stress and inflammation (DOI score) are independently correlated with glycated hemoglobin (HbA1c) in patients with prediabetes and type 2 diabetes. in Archives of Medical Science. 2020;16(1):42-50.
doi:10.5114/aoms.2019.87541 .

Klisić, Aleksandra, Kavarić, Nebojša, Stanišić, Verica, Milović, Sanja, Spasojević-Kalimanovska, Vesna, Ninić, Ana, Kotur-Stevuljević, Jelena, "Endocan and a novel score for dyslipidemia, oxidative stress and inflammation (DOI score) are independently correlated with glycated hemoglobin (HbA1c) in patients with prediabetes and type 2 diabetes" in Archives of Medical Science, 16, no. 1 (2020):42-50,
https://doi.org/10.5114/aoms.2019.87541 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Abenavoli, Ludovico
AU  - Stanišić, Verica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
AU  - Ninić, Ana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3485
AB  - Studies that evaluated endocan levels in nonalcoholic fatty liver disease (NAFLD) and liver fibrosis are scarce. We aimed to explore endocan levels in relation to different stages of liver diseases, such as NAFLD, as determined with fatty liver index (FLI) and liver fibrosis, as assessed with BARD score. A total of 147 participants with FLI≥60 were compared with 64 participants with FLI <30. An FLI score was calculated using waist circumference, body mass index, gamma-glutamyl transferase and triglycerides. Patients with FLI≥60 were further divided into those with no/mild fibrosis (BARD score 0-1 point; n=23) and advanced fibrosis (BARD score 2-4 points; n=124). BARD score was calculated as follows: diabetes mellitus (1 point) + body mass index≥28 kg/m2 (1 point) + aspartate amino transferase/alanine aminotransferase ratio≥0.8 (2 points). Endocan was independent predictor for FLI and BARD score, both in univariate [OR=1.255 (95% CI= 1.104-1.426), P=0.001; OR=1.208 (95% CI=1.029- 1.419), P=0.021, respectively] and multivariate binary logistic regression analysis [OR=1.287 (95% CI=1.055- 1.570), P=0.013; OR=1.226 (95% CI=1.022-1.470), P=0.028, respectively]. Endocan as a single predictor showed poor discriminatory capability for steatosis/fibrosis [AUC=0.648; (95% CI=0.568-0.727), P=0.002; AUC= 0.667 (95% CI=0.555-0.778), P=0.013, respectively], whereas in a Model, endocan showed an excellent clinical accuracy [AUC=0.930; (95% CI=0.886-0.975), P<0.001, AUC=0.840 (95% CI=0.763-0.918), P<0.001, respectively]. Endocan independently correlated with both FLI and BARD score. However, when tested in models (with other biomarkers), endocan showed better discriminatory ability for liver steatosis/fibrosis, instead of its usage as a single biomarker
AB  - Uvod: Nema mnogo studija koje su ispitivale vrednosti endokana kod obolelih od nealkoholne steatoze i fibroze jetre. Naš cilj je bio da se ispita nivo endokana u različitim stadijumima oboljenja jetre, kao što su nealkoholna steatoza jetre, predstavljena indeksom masne jetre (FLI) i fibroza jetre, predstavljena BARD skorom. Metode: Ukupno 147 učesnika sa FLI≥60 poređeno je sa 64 učesnika sa FLI <30. FLI skor je izračunat koriste i vrednosti obim struka, indeksa telesne mase, aktivnosti gama-glutamil transferaze i vrednosti triglicerida. Ispitanici sa FLI≥60 su dalje podeljeni u 2 grupe: bez fibroze/blaga fibroza (BARD skor 0–1 poen; n=23) i uznapredovala fibroza (BARD skor 2–4 poena; n=124). BARD skor je računat na sledeći način: e erna bolest (1 poen) + indeks telesne mase≥28 kg/m2 (1 poen) + odnos aspartat aminotransferaza/alanin aminotransferaza≥0,8 (2 poena). Rezultati: Endokan je nezavisan prediktor FLI i BARD skora, kako u univarijantnoj [OR=1,255 (95% CI=1,104– 1,426), P=0,001; odnosno OR=1,208 (95% CI=1,029– 1,419), P=0,021], tako i u multivarijantnoj binarnoj logističkoj regresionoj analizi [OR=1.287 (95% CI=1,055– 1,570), P=0,013; odnosno OR=1,226 (95% CI=1,022– 1,470), P=0,028]. Endokan kao samostalan prediktor pokazao je slabu diskriminatornu mo za steatozu/fibrozu jetre [AUC=0,648; (95% CI=0,568–0,727), P=0,002; odnosno AUC=0,667 (95% CI=0,555–0,778), P=0,013], ali je u Modelu pokazao odličnu kliničku tačnost [AUC=0,930; (95% CI=0,886–0,975), P<0,001; odnosno AUC=0,840 (95% CI=0,763–0,918), P<0,001]. Zaključak: Endokan je nezavisno povezan kako sa FLI, tako i sa BARD skorom. Ipak, u modelu (sa drugim biomarkerima), endokan je pokazao bolju diskriminatornu sposobnost za steatozu/fibrozu jetre.
PB  - Belgrade: Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Is endocan a novel potential biomarker of liver steatosis and fibrosis?
T1  - Da li je endokan novi potencijalni biomarker za steatozu i fibrozu jetre?
VL  - 39
IS  - 3
SP  - 363
EP  - 371
DO  - 10.2478/jomb-2019-0042
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Abenavoli, Ludovico and Stanišić, Verica and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena and Ninić, Ana",
year = "2020",
abstract = "Studies that evaluated endocan levels in nonalcoholic fatty liver disease (NAFLD) and liver fibrosis are scarce. We aimed to explore endocan levels in relation to different stages of liver diseases, such as NAFLD, as determined with fatty liver index (FLI) and liver fibrosis, as assessed with BARD score. A total of 147 participants with FLI≥60 were compared with 64 participants with FLI <30. An FLI score was calculated using waist circumference, body mass index, gamma-glutamyl transferase and triglycerides. Patients with FLI≥60 were further divided into those with no/mild fibrosis (BARD score 0-1 point; n=23) and advanced fibrosis (BARD score 2-4 points; n=124). BARD score was calculated as follows: diabetes mellitus (1 point) + body mass index≥28 kg/m2 (1 point) + aspartate amino transferase/alanine aminotransferase ratio≥0.8 (2 points). Endocan was independent predictor for FLI and BARD score, both in univariate [OR=1.255 (95% CI= 1.104-1.426), P=0.001; OR=1.208 (95% CI=1.029- 1.419), P=0.021, respectively] and multivariate binary logistic regression analysis [OR=1.287 (95% CI=1.055- 1.570), P=0.013; OR=1.226 (95% CI=1.022-1.470), P=0.028, respectively]. Endocan as a single predictor showed poor discriminatory capability for steatosis/fibrosis [AUC=0.648; (95% CI=0.568-0.727), P=0.002; AUC= 0.667 (95% CI=0.555-0.778), P=0.013, respectively], whereas in a Model, endocan showed an excellent clinical accuracy [AUC=0.930; (95% CI=0.886-0.975), P<0.001, AUC=0.840 (95% CI=0.763-0.918), P<0.001, respectively]. Endocan independently correlated with both FLI and BARD score. However, when tested in models (with other biomarkers), endocan showed better discriminatory ability for liver steatosis/fibrosis, instead of its usage as a single biomarker, Uvod: Nema mnogo studija koje su ispitivale vrednosti endokana kod obolelih od nealkoholne steatoze i fibroze jetre. Naš cilj je bio da se ispita nivo endokana u različitim stadijumima oboljenja jetre, kao što su nealkoholna steatoza jetre, predstavljena indeksom masne jetre (FLI) i fibroza jetre, predstavljena BARD skorom. Metode: Ukupno 147 učesnika sa FLI≥60 poređeno je sa 64 učesnika sa FLI <30. FLI skor je izračunat koriste i vrednosti obim struka, indeksa telesne mase, aktivnosti gama-glutamil transferaze i vrednosti triglicerida. Ispitanici sa FLI≥60 su dalje podeljeni u 2 grupe: bez fibroze/blaga fibroza (BARD skor 0–1 poen; n=23) i uznapredovala fibroza (BARD skor 2–4 poena; n=124). BARD skor je računat na sledeći način: e erna bolest (1 poen) + indeks telesne mase≥28 kg/m2 (1 poen) + odnos aspartat aminotransferaza/alanin aminotransferaza≥0,8 (2 poena). Rezultati: Endokan je nezavisan prediktor FLI i BARD skora, kako u univarijantnoj [OR=1,255 (95% CI=1,104– 1,426), P=0,001; odnosno OR=1,208 (95% CI=1,029– 1,419), P=0,021], tako i u multivarijantnoj binarnoj logističkoj regresionoj analizi [OR=1.287 (95% CI=1,055– 1,570), P=0,013; odnosno OR=1,226 (95% CI=1,022– 1,470), P=0,028]. Endokan kao samostalan prediktor pokazao je slabu diskriminatornu mo za steatozu/fibrozu jetre [AUC=0,648; (95% CI=0,568–0,727), P=0,002; odnosno AUC=0,667 (95% CI=0,555–0,778), P=0,013], ali je u Modelu pokazao odličnu kliničku tačnost [AUC=0,930; (95% CI=0,886–0,975), P<0,001; odnosno AUC=0,840 (95% CI=0,763–0,918), P<0,001]. Zaključak: Endokan je nezavisno povezan kako sa FLI, tako i sa BARD skorom. Ipak, u modelu (sa drugim biomarkerima), endokan je pokazao bolju diskriminatornu sposobnost za steatozu/fibrozu jetre.",
publisher = "Belgrade: Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Is endocan a novel potential biomarker of liver steatosis and fibrosis?, Da li je endokan novi potencijalni biomarker za steatozu i fibrozu jetre?",
volume = "39",
number = "3",
pages = "363-371",
doi = "10.2478/jomb-2019-0042"
}

Klisić, A., Kavarić, N., Abenavoli, L., Stanišić, V., Spasojević-Kalimanovska, V., Kotur-Stevuljević, J.,& Ninić, A.. (2020). Is endocan a novel potential biomarker of liver steatosis and fibrosis?. in Journal of Medical Biochemistry
Belgrade: Society of Medical Biochemists of Serbia., 39(3), 363-371.
https://doi.org/10.2478/jomb-2019-0042

Klisić A, Kavarić N, Abenavoli L, Stanišić V, Spasojević-Kalimanovska V, Kotur-Stevuljević J, Ninić A. Is endocan a novel potential biomarker of liver steatosis and fibrosis?. in Journal of Medical Biochemistry. 2020;39(3):363-371.
doi:10.2478/jomb-2019-0042 .

Klisić, Aleksandra, Kavarić, Nebojša, Abenavoli, Ludovico, Stanišić, Verica, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, Ninić, Ana, "Is endocan a novel potential biomarker of liver steatosis and fibrosis?" in Journal of Medical Biochemistry, 39, no. 3 (2020):363-371,
https://doi.org/10.2478/jomb-2019-0042 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kocić, Gordana
AU  - Kavarić, Nebojša
AU  - Jovanović, M
AU  - Stanišić, Verica
AU  - Ninić, Ana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3142
AB  - Purpose: The pathophysiological mechanism of the relationship between xanthine oxidase (XO) activity and obesity has not been completely elucidated. Since inflammation and oxidative stress are regarded as key determinants of enlarged adipose tissue, we aimed to investigate the association between oxidative stress (as measured with XO activity), inflammation [as measured with high-sensitivity C-reactive protein (hsCRP)] and obesity [as measured with body mass index (BMI)]. In addition, we wanted to examine whether hsCRP itself plays an independent role in XO activity increase or it is only mediated through obesity. Methods: A total of 118 overweight/obese volunteers (mean age 54.76 ± 15.13 years) were included in the current cross-sectional study. Anthropometric, biochemical parameters, and blood pressure were obtained. Results: Significant differences between age, BMI, waist circumference, concentrations of uric acid and hsCRP, as well as xanthine dehydrogenase (XDH) activities were evident among XO tertile groups. Multiple linear regression analysis revealed that BMI (beta = 0.241, p = 0.012) and XDH (beta = − 0.489, p < 0.001) are the independent predictors of XO activity (R2-adjusted = 0.333), whereas hsCRP lost its independent role in XO activity prediction. Conclusion: Obesity (as determined with increased BMI) is an independent predictor of high XO activity in overweight/obese population. Level of Evidence: Level V: cross-sectional descriptive study.
PB  - Springer International Publishing
T2  - Eating and Weight Disorders
T1  - Body mass index is independently associated with xanthine oxidase activity in overweight/obese population
VL  - 25
IS  - 1
SP  - 9
EP  - 15
DO  - 10.1007/s40519-018-0490-5
ER  - 

@article{
author = "Klisić, Aleksandra and Kocić, Gordana and Kavarić, Nebojša and Jovanović, M and Stanišić, Verica and Ninić, Ana",
year = "2020",
abstract = "Purpose: The pathophysiological mechanism of the relationship between xanthine oxidase (XO) activity and obesity has not been completely elucidated. Since inflammation and oxidative stress are regarded as key determinants of enlarged adipose tissue, we aimed to investigate the association between oxidative stress (as measured with XO activity), inflammation [as measured with high-sensitivity C-reactive protein (hsCRP)] and obesity [as measured with body mass index (BMI)]. In addition, we wanted to examine whether hsCRP itself plays an independent role in XO activity increase or it is only mediated through obesity. Methods: A total of 118 overweight/obese volunteers (mean age 54.76 ± 15.13 years) were included in the current cross-sectional study. Anthropometric, biochemical parameters, and blood pressure were obtained. Results: Significant differences between age, BMI, waist circumference, concentrations of uric acid and hsCRP, as well as xanthine dehydrogenase (XDH) activities were evident among XO tertile groups. Multiple linear regression analysis revealed that BMI (beta = 0.241, p = 0.012) and XDH (beta = − 0.489, p < 0.001) are the independent predictors of XO activity (R2-adjusted = 0.333), whereas hsCRP lost its independent role in XO activity prediction. Conclusion: Obesity (as determined with increased BMI) is an independent predictor of high XO activity in overweight/obese population. Level of Evidence: Level V: cross-sectional descriptive study.",
publisher = "Springer International Publishing",
journal = "Eating and Weight Disorders",
title = "Body mass index is independently associated with xanthine oxidase activity in overweight/obese population",
volume = "25",
number = "1",
pages = "9-15",
doi = "10.1007/s40519-018-0490-5"
}

Klisić, A., Kocić, G., Kavarić, N., Jovanović, M., Stanišić, V.,& Ninić, A.. (2020). Body mass index is independently associated with xanthine oxidase activity in overweight/obese population. in Eating and Weight Disorders
Springer International Publishing., 25(1), 9-15.
https://doi.org/10.1007/s40519-018-0490-5

Klisić A, Kocić G, Kavarić N, Jovanović M, Stanišić V, Ninić A. Body mass index is independently associated with xanthine oxidase activity in overweight/obese population. in Eating and Weight Disorders. 2020;25(1):9-15.
doi:10.1007/s40519-018-0490-5 .

Klisić, Aleksandra, Kocić, Gordana, Kavarić, Nebojša, Jovanović, M, Stanišić, Verica, Ninić, Ana, "Body mass index is independently associated with xanthine oxidase activity in overweight/obese population" in Eating and Weight Disorders, 25, no. 1 (2020):9-15,
https://doi.org/10.1007/s40519-018-0490-5 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Bjelaković, Bojko
AU  - Jovanović, Milovan
AU  - Zvrko, Elvir
AU  - Stanišić, Verica
AU  - Ninić, Ana
AU  - Šćepanović, Anđelka
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3239
AB  - Reynolds Risk Score (RRS) is regarded as a good screening tool for cardiovascular disease (CVD) risk. Since CVD is the leading cause of death in Montenegro, we aimed to assess the risk of CVD as assessed by RRS and to examine its association with cardiometabolic parameters in apparently healthy middle-aged population. In addition, we aimed to test whether obesity had an independent influence on RRS. A total of 132 participants (mean age 56.2 +/- 6.73 years, 69% females) were included. Body mass index (BMI), waist circumference (WC), blood pressure (BP) and biochemical parameters (fasting glucose, insulin, lipid parameters, creatinine and high sensitivity C-reactive protein) were determined. Insulin resistance (HOMA-IR) and glomerular filtration rate (eGFR) were calculated. Compared with females, a significantly higher number of males were in the high RRS subgroup (chi(2) =45.9, p lt 0.001). Furthermore, significantly higher fasting glucose (p=0.030), insulin, HOMA-IR, triglycerides (p lt 0.001 all), anthropometric parameters (e.g., BMI and WC; p=0.004 and p lt 0.001, respectively), and creatinine, but lower eGFR and HDL-c (p lt 0.001 both) were recorded in the high-risk subgroup compared with low and medium risk subgroups. In all participants, in addition to LDL-c, diastolic BP and creatinine, WC was independently positively associated with RRS (beta=0.194, p=0.006; lt b> beta=0186, p=0.001; beta=0167, p=0.001; and beta=0.305, p=0.019, respectively), and 40% of variation in RRS could be explained with this model. In conclusion, middle-aged population with higher WC should be screened for RRS in order to estimate CVD risk.
PB  - Sestre Milosrdnice Univ Hospital, Zagreb
T2  - Acta Clinica Croatica
T1  - Cardiovascular risk assessed by reynolds risk score in relation to waist circumference in apparently healthy middle-aged population in Montenegro
VL  - 57
IS  - 1
SP  - 22
EP  - 30
DO  - 10.20471/acc.2018.57.01.03
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Bjelaković, Bojko and Jovanović, Milovan and Zvrko, Elvir and Stanišić, Verica and Ninić, Ana and Šćepanović, Anđelka",
year = "2018",
abstract = "Reynolds Risk Score (RRS) is regarded as a good screening tool for cardiovascular disease (CVD) risk. Since CVD is the leading cause of death in Montenegro, we aimed to assess the risk of CVD as assessed by RRS and to examine its association with cardiometabolic parameters in apparently healthy middle-aged population. In addition, we aimed to test whether obesity had an independent influence on RRS. A total of 132 participants (mean age 56.2 +/- 6.73 years, 69% females) were included. Body mass index (BMI), waist circumference (WC), blood pressure (BP) and biochemical parameters (fasting glucose, insulin, lipid parameters, creatinine and high sensitivity C-reactive protein) were determined. Insulin resistance (HOMA-IR) and glomerular filtration rate (eGFR) were calculated. Compared with females, a significantly higher number of males were in the high RRS subgroup (chi(2) =45.9, p lt 0.001). Furthermore, significantly higher fasting glucose (p=0.030), insulin, HOMA-IR, triglycerides (p lt 0.001 all), anthropometric parameters (e.g., BMI and WC; p=0.004 and p lt 0.001, respectively), and creatinine, but lower eGFR and HDL-c (p lt 0.001 both) were recorded in the high-risk subgroup compared with low and medium risk subgroups. In all participants, in addition to LDL-c, diastolic BP and creatinine, WC was independently positively associated with RRS (beta=0.194, p=0.006; lt b> beta=0186, p=0.001; beta=0167, p=0.001; and beta=0.305, p=0.019, respectively), and 40% of variation in RRS could be explained with this model. In conclusion, middle-aged population with higher WC should be screened for RRS in order to estimate CVD risk.",
publisher = "Sestre Milosrdnice Univ Hospital, Zagreb",
journal = "Acta Clinica Croatica",
title = "Cardiovascular risk assessed by reynolds risk score in relation to waist circumference in apparently healthy middle-aged population in Montenegro",
volume = "57",
number = "1",
pages = "22-30",
doi = "10.20471/acc.2018.57.01.03"
}

Klisić, A., Kavarić, N., Bjelaković, B., Jovanović, M., Zvrko, E., Stanišić, V., Ninić, A.,& Šćepanović, A.. (2018). Cardiovascular risk assessed by reynolds risk score in relation to waist circumference in apparently healthy middle-aged population in Montenegro. in Acta Clinica Croatica
Sestre Milosrdnice Univ Hospital, Zagreb., 57(1), 22-30.
https://doi.org/10.20471/acc.2018.57.01.03

Klisić A, Kavarić N, Bjelaković B, Jovanović M, Zvrko E, Stanišić V, Ninić A, Šćepanović A. Cardiovascular risk assessed by reynolds risk score in relation to waist circumference in apparently healthy middle-aged population in Montenegro. in Acta Clinica Croatica. 2018;57(1):22-30.
doi:10.20471/acc.2018.57.01.03 .

Klisić, Aleksandra, Kavarić, Nebojša, Bjelaković, Bojko, Jovanović, Milovan, Zvrko, Elvir, Stanišić, Verica, Ninić, Ana, Šćepanović, Anđelka, "Cardiovascular risk assessed by reynolds risk score in relation to waist circumference in apparently healthy middle-aged population in Montenegro" in Acta Clinica Croatica, 57, no. 1 (2018):22-30,
https://doi.org/10.20471/acc.2018.57.01.03 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kocić, Gordana
AU  - Kavarić, Nebojša
AU  - Jovanović, Milovan
AU  - Stanišić, Verica
AU  - Ninić, Ana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3161
AB  - Xanthine oxidase (XO) is an important enzyme responsible for conversion of purine bases to uric acid and represents the major source of reactive oxygen species (ROS) production in circulation. Since pathophysiological mechanism of the relationship between XO activity and urinary albumin excretion (UAE) rate is not well elucidated, we aimed to investigate this association in patients with diabetes mellitus type 2 (DM2). In addition, we wanted to examine whether uric acid itself plays an independent role in albuminuria onset and progression, or it is only mediated through XO activity. A total of 83 patients with DM2 (of them 56.6% females) were included in this cross-sectional study. Anthropometric, biochemical parameters and blood pressure were obtained. Multivariate logistic regression analysis showed that uric acid and XO were the independent predictors for albuminuria onset in patients with DM2 [odds ratio (OR) 1.015, 95% CI (1.008-1.028), p = 0.026 and OR 1.015, 95% CI (1.006-1.026), p = 0.040, respectively]. Rise in uric acid for 1 mu mol/L enhanced the probability for albuminuria by 1.5%. Also, elevation in XO activity for 1 U/L increased the probability for albuminuria for 1.5%. A total of 66.7% of variation in UAE could be explained with this Model. Both XO and uric acid are independently associated with albuminuria in diabetes. Better understanding of pathophysiological relationship between oxidative stress and albuminuria could lead to discoveries of best pharmacological treatment of XO- and/or uric acid-induced ROS, in order to prevent albuminuria onset and progression.
PB  - Springer-Verlag Italia Srl, Milan
T2  - Clinical and Experimental Medicine
T1  - Xanthine oxidase and uric acid as independent predictors of albuminuria in patients with diabetes mellitus type 2
VL  - 18
IS  - 2
SP  - 283
EP  - 290
DO  - 10.1007/s10238-017-0483-0
ER  - 

@article{
author = "Klisić, Aleksandra and Kocić, Gordana and Kavarić, Nebojša and Jovanović, Milovan and Stanišić, Verica and Ninić, Ana",
year = "2018",
abstract = "Xanthine oxidase (XO) is an important enzyme responsible for conversion of purine bases to uric acid and represents the major source of reactive oxygen species (ROS) production in circulation. Since pathophysiological mechanism of the relationship between XO activity and urinary albumin excretion (UAE) rate is not well elucidated, we aimed to investigate this association in patients with diabetes mellitus type 2 (DM2). In addition, we wanted to examine whether uric acid itself plays an independent role in albuminuria onset and progression, or it is only mediated through XO activity. A total of 83 patients with DM2 (of them 56.6% females) were included in this cross-sectional study. Anthropometric, biochemical parameters and blood pressure were obtained. Multivariate logistic regression analysis showed that uric acid and XO were the independent predictors for albuminuria onset in patients with DM2 [odds ratio (OR) 1.015, 95% CI (1.008-1.028), p = 0.026 and OR 1.015, 95% CI (1.006-1.026), p = 0.040, respectively]. Rise in uric acid for 1 mu mol/L enhanced the probability for albuminuria by 1.5%. Also, elevation in XO activity for 1 U/L increased the probability for albuminuria for 1.5%. A total of 66.7% of variation in UAE could be explained with this Model. Both XO and uric acid are independently associated with albuminuria in diabetes. Better understanding of pathophysiological relationship between oxidative stress and albuminuria could lead to discoveries of best pharmacological treatment of XO- and/or uric acid-induced ROS, in order to prevent albuminuria onset and progression.",
publisher = "Springer-Verlag Italia Srl, Milan",
journal = "Clinical and Experimental Medicine",
title = "Xanthine oxidase and uric acid as independent predictors of albuminuria in patients with diabetes mellitus type 2",
volume = "18",
number = "2",
pages = "283-290",
doi = "10.1007/s10238-017-0483-0"
}

Klisić, A., Kocić, G., Kavarić, N., Jovanović, M., Stanišić, V.,& Ninić, A.. (2018). Xanthine oxidase and uric acid as independent predictors of albuminuria in patients with diabetes mellitus type 2. in Clinical and Experimental Medicine
Springer-Verlag Italia Srl, Milan., 18(2), 283-290.
https://doi.org/10.1007/s10238-017-0483-0

Klisić A, Kocić G, Kavarić N, Jovanović M, Stanišić V, Ninić A. Xanthine oxidase and uric acid as independent predictors of albuminuria in patients with diabetes mellitus type 2. in Clinical and Experimental Medicine. 2018;18(2):283-290.
doi:10.1007/s10238-017-0483-0 .

Klisić, Aleksandra, Kocić, Gordana, Kavarić, Nebojša, Jovanović, Milovan, Stanišić, Verica, Ninić, Ana, "Xanthine oxidase and uric acid as independent predictors of albuminuria in patients with diabetes mellitus type 2" in Clinical and Experimental Medicine, 18, no. 2 (2018):283-290,
https://doi.org/10.1007/s10238-017-0483-0 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Stanišić, Verica
AU  - Jovanović, Milovan
AU  - Kavarić, Nebojša
AU  - Ninić, Ana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2973
AB  - Aim: Retinol-binding protein 4 (RBP4) is a novel adipokine closely related to insulin resistance. However, data on the influence of menopausal status on serum RBP4 are scarce. Therefore, the aim of the current study was to examine whether RBP4 levels are associated with menopausal status per se, independently of insulin resistance. Methods: A total of 30 premenopausal and 100 postmenopausal women non-treated with medications were included in the cross-sectional study. Anthropometric and biochemical parameters, as well as blood pressure (BP) were obtained. The homeostasis model assessment of insulin resistance (HOMA-IR) and estimated glomerular filtration rate (eGFR) were calculated. Results: Postmenopausal women displayed higher RBP4 and an unfavorable cardiometabolic profile, compared to premenopausal ones. Multiple linear regression analysis showed that in addition to high triglycerides level (beta=0.315; p=0.002), decreased eGFR (beta=-0.258; p=0.004) and high systolic BP (beta=0.418; p=0.028), menopause per se is an independent predictor of higher RBP4 levels (beta=0.240; p=0.016), (R2-adjusted=0.310; F=6,522; p lt 0.001). Conclusions: Serum RBP4 levels are dependent of menopausal status, which should be taken into account when examining the role of this adipokine in cardiometabolic diseases' occurrence.
AB  - Cilj: Retinol-vezujući protein 4 (RBP4) je novi adipokin, usko povezan sa insulinskom rezistencijom. Međutim, nema dovoljno podataka u literaturi o uticaju menopauze na vrednosti ovog proteina u serumu. Zato je cilj ove studije bio da se ispita da li je povezanost menopauze i RBP4 nezavisna ili je posredovana insulinskom rezistencijom. Metode: Ukupno 30 žena u premenopauzi i 100 žena u postmenopauzi, koje nisu na terapiji su uključene u studiju preseka. Mereni su antropometrijski i biohemijski parametri, kao i krvni pritisak (KP), HOMA indeksi, procenjena jačina glomerularne filtracije (JGF) i izračunati su. Rezultati: Kod žena u postmenopauzi zabeležene su veće vrednosti RBP4 i nepovoljniji kardiometabolički profil, u poređenju sa ženama u premenopauzi. Višestruka linearnaregresiona analiza je pokazala da su više vrednosti triglicerida (beta=0,315; p=0,002), smanjena JGF (beta=-0,258; p=0,004), više vrednosti sistolnog KP (beta=0,418; p=0,028), i menopauza (beta=0,240; p=0,016), nezavisni prediktori povišenih vrednosti RBP4 u serumu (R2-adjusted=0,310; F=6,522; p lt 0,001). Zaključak: Menopauza utiče na vrednosti RBP4 u serumu, što treba uzeti u obzir prilikom ispitivanja uloge ovog adipokina u pojavi kardiometaboličkih poremećaja.
PB  - Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar
T2  - Timočki medicinski glasnik
T1  - Menopausal status as an independent predictor of high serum retinol-binding protein 4 levels
T1  - Menopauza kao nezavisni prediktor povišenih vrednosti retinolvezujućeg proteina 4 u serumu
VL  - 42
IS  - 4
SP  - 199
EP  - 205
DO  - 10.5937/tmg1704199K
ER  - 

@article{
author = "Klisić, Aleksandra and Stanišić, Verica and Jovanović, Milovan and Kavarić, Nebojša and Ninić, Ana",
year = "2017",
abstract = "Aim: Retinol-binding protein 4 (RBP4) is a novel adipokine closely related to insulin resistance. However, data on the influence of menopausal status on serum RBP4 are scarce. Therefore, the aim of the current study was to examine whether RBP4 levels are associated with menopausal status per se, independently of insulin resistance. Methods: A total of 30 premenopausal and 100 postmenopausal women non-treated with medications were included in the cross-sectional study. Anthropometric and biochemical parameters, as well as blood pressure (BP) were obtained. The homeostasis model assessment of insulin resistance (HOMA-IR) and estimated glomerular filtration rate (eGFR) were calculated. Results: Postmenopausal women displayed higher RBP4 and an unfavorable cardiometabolic profile, compared to premenopausal ones. Multiple linear regression analysis showed that in addition to high triglycerides level (beta=0.315; p=0.002), decreased eGFR (beta=-0.258; p=0.004) and high systolic BP (beta=0.418; p=0.028), menopause per se is an independent predictor of higher RBP4 levels (beta=0.240; p=0.016), (R2-adjusted=0.310; F=6,522; p lt 0.001). Conclusions: Serum RBP4 levels are dependent of menopausal status, which should be taken into account when examining the role of this adipokine in cardiometabolic diseases' occurrence., Cilj: Retinol-vezujući protein 4 (RBP4) je novi adipokin, usko povezan sa insulinskom rezistencijom. Međutim, nema dovoljno podataka u literaturi o uticaju menopauze na vrednosti ovog proteina u serumu. Zato je cilj ove studije bio da se ispita da li je povezanost menopauze i RBP4 nezavisna ili je posredovana insulinskom rezistencijom. Metode: Ukupno 30 žena u premenopauzi i 100 žena u postmenopauzi, koje nisu na terapiji su uključene u studiju preseka. Mereni su antropometrijski i biohemijski parametri, kao i krvni pritisak (KP), HOMA indeksi, procenjena jačina glomerularne filtracije (JGF) i izračunati su. Rezultati: Kod žena u postmenopauzi zabeležene su veće vrednosti RBP4 i nepovoljniji kardiometabolički profil, u poređenju sa ženama u premenopauzi. Višestruka linearnaregresiona analiza je pokazala da su više vrednosti triglicerida (beta=0,315; p=0,002), smanjena JGF (beta=-0,258; p=0,004), više vrednosti sistolnog KP (beta=0,418; p=0,028), i menopauza (beta=0,240; p=0,016), nezavisni prediktori povišenih vrednosti RBP4 u serumu (R2-adjusted=0,310; F=6,522; p lt 0,001). Zaključak: Menopauza utiče na vrednosti RBP4 u serumu, što treba uzeti u obzir prilikom ispitivanja uloge ovog adipokina u pojavi kardiometaboličkih poremećaja.",
publisher = "Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar",
journal = "Timočki medicinski glasnik",
title = "Menopausal status as an independent predictor of high serum retinol-binding protein 4 levels, Menopauza kao nezavisni prediktor povišenih vrednosti retinolvezujućeg proteina 4 u serumu",
volume = "42",
number = "4",
pages = "199-205",
doi = "10.5937/tmg1704199K"
}

Klisić, A., Stanišić, V., Jovanović, M., Kavarić, N.,& Ninić, A.. (2017). Menopausal status as an independent predictor of high serum retinol-binding protein 4 levels. in Timočki medicinski glasnik
Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar., 42(4), 199-205.
https://doi.org/10.5937/tmg1704199K

Klisić A, Stanišić V, Jovanović M, Kavarić N, Ninić A. Menopausal status as an independent predictor of high serum retinol-binding protein 4 levels. in Timočki medicinski glasnik. 2017;42(4):199-205.
doi:10.5937/tmg1704199K .

Klisić, Aleksandra, Stanišić, Verica, Jovanović, Milovan, Kavarić, Nebojša, Ninić, Ana, "Menopausal status as an independent predictor of high serum retinol-binding protein 4 levels" in Timočki medicinski glasnik, 42, no. 4 (2017):199-205,
https://doi.org/10.5937/tmg1704199K . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Stanišić, Verica
AU  - Jovanović, Milovan
AU  - Kavarić, Nebojša
AU  - Ninić, Ana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2914
AB  - Aim: Although previous studies confirmed the association between obesity and hypertension, the underlying pathophysiological mechanism is not well elucidated, specially considering the fact that discordant results exist when examining the independent role of obesity and/or insulin resistance on risk for hypertension onset. Therefore, we aimed to examine if obesity [as measured with body mass index (BMI)] is a predictor for hypertension, independently of insulin resistance [as measured with Homeostasis model assessment of insulin resistance (HOMA-IR)] in the cohort of postmenopausal women. Methods: A total of 150 postmenopausal women non-treated with medications(among them 44.7% hypertensive)were included in cross-sectional study. Anthropometric and biochemical parameters, so as blood pressure were obtained. HOMA-IR was calculated. Results: Multivariate logistic regression analysisrevealed that both, BMI and HOMA-IR were the independent predictors of blood pressure in postmenopausal women (OR=1.240, p=0.035 and OR=2.419, p=0.008, respectively). Rise in BMI for 1 kg/m2 enhanced the probability for higher blood pressure by 24%. Also, elevation in HOMA-IR for 1 unit, rose the probability for higher blood pressure almost 2.5 times. Even 47% of variation in blood pressure could be explained with this Model. Also, this Model correctly classified 76% of postmenopausal women having hypertension. Conclusions: Both, obesity and insulin resistance are the independent predictors of blood pressure in postmenopausal women.
AB  - Cilj: Premda su ranije studije potvrdile vezu između gojaznosti i hipertenzije, patofiziološki mehanizam ove povezanosti još uvek nije rasvetljen, naročito što postoje oprečni rezultati kada je u pitanju nezavisna uloga gojaznosti i/ili insulinske rezistencije u nastanku hipertenzije. Stoga je cilj studije bio da se ispita da li je gojaznost [merena indeksom telesne mase (ITM)] prediktor za nastanak hipertenzije, nezavisno od insulinske rezistencije [merene HOMA indeksom (HOMA-IR)] u kohorti žena u postmenopauzi. Metode: Ukupno 150 žena u postmenopauzi koje nisu na terapiji (među njima 44,7% sa hipertenzijom) su uključene u studiju preseka. Antropometrijski i biohemijski parametri, kao i krvni pritisak su mereni. HOMA-IR je računat. Rezultati: Multivarijantna logistička regresiona analiza je pokazala da su i ITM (OR=1,240; p=0,035) i HOMA-IR (OR=2,419; p=0,008)nezavisni prediktori krvnog pritiska kod žena u postmenopauzi. Povećanje ITMza1 kg/m2 povećava verovatnoću za pojavu hipertenzije za 24%. Takođe, porast HOMA-IR za 1 jedinicu povećava verovatnoću za pojavu hipertenzije za skoro 2,5 puta. Čak 47% varijacije u vrednosti krvnog pritiska može biti objašnjeno ovim modelom. Takođe, ovaj model je ispravno klasifikovao 76% žena u postmenopauzi koje imaju hipertenziju. Zaključak: I gojaznost, i insulinska rezistencija su nezavisni prediktori krvnog pritiska kod žena u postmenopauzi.
PB  - Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar
T2  - Timočki medicinski glasnik
T1  - Body mass index and insulin resistance as independent predictors of hypertension in postmenopausal women
T1  - Indeks telesne mase i insulinska rezistencija kao nezavisni prediktori hipertenzije kod žena u postmenopauzi
VL  - 42
IS  - 3
SP  - 165
EP  - 172
DO  - 10.5937/tmg1703165K
ER  - 

@article{
author = "Klisić, Aleksandra and Stanišić, Verica and Jovanović, Milovan and Kavarić, Nebojša and Ninić, Ana",
year = "2017",
abstract = "Aim: Although previous studies confirmed the association between obesity and hypertension, the underlying pathophysiological mechanism is not well elucidated, specially considering the fact that discordant results exist when examining the independent role of obesity and/or insulin resistance on risk for hypertension onset. Therefore, we aimed to examine if obesity [as measured with body mass index (BMI)] is a predictor for hypertension, independently of insulin resistance [as measured with Homeostasis model assessment of insulin resistance (HOMA-IR)] in the cohort of postmenopausal women. Methods: A total of 150 postmenopausal women non-treated with medications(among them 44.7% hypertensive)were included in cross-sectional study. Anthropometric and biochemical parameters, so as blood pressure were obtained. HOMA-IR was calculated. Results: Multivariate logistic regression analysisrevealed that both, BMI and HOMA-IR were the independent predictors of blood pressure in postmenopausal women (OR=1.240, p=0.035 and OR=2.419, p=0.008, respectively). Rise in BMI for 1 kg/m2 enhanced the probability for higher blood pressure by 24%. Also, elevation in HOMA-IR for 1 unit, rose the probability for higher blood pressure almost 2.5 times. Even 47% of variation in blood pressure could be explained with this Model. Also, this Model correctly classified 76% of postmenopausal women having hypertension. Conclusions: Both, obesity and insulin resistance are the independent predictors of blood pressure in postmenopausal women., Cilj: Premda su ranije studije potvrdile vezu između gojaznosti i hipertenzije, patofiziološki mehanizam ove povezanosti još uvek nije rasvetljen, naročito što postoje oprečni rezultati kada je u pitanju nezavisna uloga gojaznosti i/ili insulinske rezistencije u nastanku hipertenzije. Stoga je cilj studije bio da se ispita da li je gojaznost [merena indeksom telesne mase (ITM)] prediktor za nastanak hipertenzije, nezavisno od insulinske rezistencije [merene HOMA indeksom (HOMA-IR)] u kohorti žena u postmenopauzi. Metode: Ukupno 150 žena u postmenopauzi koje nisu na terapiji (među njima 44,7% sa hipertenzijom) su uključene u studiju preseka. Antropometrijski i biohemijski parametri, kao i krvni pritisak su mereni. HOMA-IR je računat. Rezultati: Multivarijantna logistička regresiona analiza je pokazala da su i ITM (OR=1,240; p=0,035) i HOMA-IR (OR=2,419; p=0,008)nezavisni prediktori krvnog pritiska kod žena u postmenopauzi. Povećanje ITMza1 kg/m2 povećava verovatnoću za pojavu hipertenzije za 24%. Takođe, porast HOMA-IR za 1 jedinicu povećava verovatnoću za pojavu hipertenzije za skoro 2,5 puta. Čak 47% varijacije u vrednosti krvnog pritiska može biti objašnjeno ovim modelom. Takođe, ovaj model je ispravno klasifikovao 76% žena u postmenopauzi koje imaju hipertenziju. Zaključak: I gojaznost, i insulinska rezistencija su nezavisni prediktori krvnog pritiska kod žena u postmenopauzi.",
publisher = "Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar",
journal = "Timočki medicinski glasnik",
title = "Body mass index and insulin resistance as independent predictors of hypertension in postmenopausal women, Indeks telesne mase i insulinska rezistencija kao nezavisni prediktori hipertenzije kod žena u postmenopauzi",
volume = "42",
number = "3",
pages = "165-172",
doi = "10.5937/tmg1703165K"
}

Klisić, A., Stanišić, V., Jovanović, M., Kavarić, N.,& Ninić, A.. (2017). Body mass index and insulin resistance as independent predictors of hypertension in postmenopausal women. in Timočki medicinski glasnik
Srpsko lekarsko društvo - Podružnica Zaječar, Zaječar., 42(3), 165-172.
https://doi.org/10.5937/tmg1703165K

Klisić A, Stanišić V, Jovanović M, Kavarić N, Ninić A. Body mass index and insulin resistance as independent predictors of hypertension in postmenopausal women. in Timočki medicinski glasnik. 2017;42(3):165-172.
doi:10.5937/tmg1703165K .

Klisić, Aleksandra, Stanišić, Verica, Jovanović, Milovan, Kavarić, Nebojša, Ninić, Ana, "Body mass index and insulin resistance as independent predictors of hypertension in postmenopausal women" in Timočki medicinski glasnik, 42, no. 3 (2017):165-172,
https://doi.org/10.5937/tmg1703165K . .