TY  - JOUR
AU  - Sajc, L
AU  - Vunjak-Novaković, G
AU  - Grubišić, Dragoljub
AU  - Kovačević, Nada
AU  - Vuković, D
AU  - Bugarski, Branko
PY  - 1995
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/126
AB  - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
T2  - Applied Microbiology and Biotechnology
T1  - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
VL  - 43
IS  - 3
SP  - 416
EP  - 423
DO  - 10.1007/BF00218443
ER  - 

@article{
author = "Sajc, L and Vunjak-Novaković, G and Grubišić, Dragoljub and Kovačević, Nada and Vuković, D and Bugarski, Branko",
year = "1995",
abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.",
journal = "Applied Microbiology and Biotechnology",
title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor",
volume = "43",
number = "3",
pages = "416-423",
doi = "10.1007/BF00218443"
}

Sajc, L., Vunjak-Novaković, G., Grubišić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology, 43(3), 416-423.
https://doi.org/10.1007/BF00218443

Sajc L, Vunjak-Novaković G, Grubišić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423.
doi:10.1007/BF00218443 .

Sajc, L, Vunjak-Novaković, G, Grubišić, Dragoljub, Kovačević, Nada, Vuković, D, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423,
https://doi.org/10.1007/BF00218443 . .