TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana J.
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Lozance, Olivera
AU  - Blagojević, Miloš
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/901
AB  - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
VL  - 30
IS  - 4
SP  - 1144
EP  - 1149
DO  - 10.1590/S1415-47572007000600019
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana J. and Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozance, Olivera and Blagojević, Miloš",
year = "2007",
abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes",
volume = "30",
number = "4",
pages = "1144-1149",
doi = "10.1590/S1415-47572007000600019"
}

Đelić, N., Đelić, D. J., Potparević, B., Živković, L., Marković, B., Lozance, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149.
https://doi.org/10.1590/S1415-47572007000600019

Đelić N, Đelić DJ, Potparević B, Živković L, Marković B, Lozance O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149.
doi:10.1590/S1415-47572007000600019 .

Đelić, Ninoslav, Đelić, Dijana J., Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozance, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149,
https://doi.org/10.1590/S1415-47572007000600019 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
AU  - Marković, Biljana
AU  - Živković, Lada
AU  - Đelić, Dijana J.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/868
AB  - Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level.
AB  - Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro
T1  - Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro
VL  - 56
IS  - 1
SP  - 37
EP  - 48
DO  - 10.2298/AVB0601037D
ER  - 

@article{
author = "Đelić, Ninoslav and Spremo-Potparević, Biljana and Marković, Biljana and Živković, Lada and Đelić, Dijana J.",
year = "2006",
abstract = "Metabolic conversion of oestrogen phenolic groups may create conditions of oxidative stress accompanied by damage of cellular macromolecules including DNA. The aim of this investigation was to evaluate the cell cycle kinetics and possible cytogenetic changes in cultured human peripheral blood lymphocytes exposed to seven experimental concentrations of 17β-oestradiol (range 10-10 M to 10-4 M). Cell cycle kinetics was analyzed on metaphase spreads prepared for a standard analysis of sister-chromatid exchanges (SCEs) stained by fluorescent-plus-Giemsa (FPG) technique. Cytogenetic changes were monitored by analysis of chromosome damage (gaps and breaks), structural and numerical aberrations. On the basis of the obtained results it can be concluded that oestradiol has no significant influence on cell cycle kinetics and mitotic index of cultured human lymphocytes. However, at estradiol concentration of 7×10-6 M, and at higher concentrations used in this experiment, there was a significant increase of gaps, breaks and aneuploidies. On the other hand, oestradiol treatment has not changed the frequency of polyploid cells. Therefore, it can be concluded that high concentrations of oestradiol pose some genetic risk detectable at cytogenetic level., Metabolička konverzija fenolnih grupa estrogenih hormona može da dovede do oksidativnog stresa praćenog oštećenjima različitih makromolekula u eliji, uključujući DNK. Cilj ovog istraživanja je evaluacija kinetike proliferacije i mogućih citogenetičkih promena u kulturama humanih limfocita pod dejstvom sedam eksperimentalnih koncentracija 17β-estradiola (opseg od 10-10M do10-4 M). Kinetika proliferacije limfocita analizirana je na metafaznim figurama obojenim tehnikom FPG za standardne analize razmena sestrinskih hromatida (SCE). Citogenetičke promene praćene su analizama hromozomskih oštećenja (gapovi i prekidi), strukturnih i numeričkih aberacija hromozoma. Na osnovu dobijenih rezultata može se zaključiti da estradiol ne utiče značajno na mitotsku aktivnost i kinetiku proliferacije limfocita u kulturi. Međutim, pri koncentraciji od 7×10-6 M, kao i pri višim eksperimentalnim koncentracijama korišćenim u ovim eksperimentima, zapažen je porast gapova, prekida i aneuploidija. S druge strane, tretman estradiolom ne menja učestalost poliploidnih ćelija. Prema tome, može se zaključiti da visoke koncentracije estradiola izazivaju izvestan genetički rizik koji se može detektovati na citogenetičkom nivou.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro, Kinetika proliferacije i citogenetičke promene u humanim limfocitima pod dejstvom estradiola in vitro",
volume = "56",
number = "1",
pages = "37-48",
doi = "10.2298/AVB0601037D"
}

Đelić, N., Spremo-Potparević, B., Marković, B., Živković, L.,& Đelić, D. J.. (2006). Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(1), 37-48.
https://doi.org/10.2298/AVB0601037D

Đelić N, Spremo-Potparević B, Marković B, Živković L, Đelić DJ. Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro. in Acta veterinaria. 2006;56(1):37-48.
doi:10.2298/AVB0601037D .

Đelić, Ninoslav, Spremo-Potparević, Biljana, Marković, Biljana, Živković, Lada, Đelić, Dijana J., "Cell cycle kinetics and cytogenetic changes in human lymphocytes exposed to oestradiol in vitro" in Acta veterinaria, 56, no. 1 (2006):37-48,
https://doi.org/10.2298/AVB0601037D . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana J.
AU  - Potparević, Biljana
AU  - Marković, Biljana
AU  - Živković, Lada
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/462
AB  - Epinephrine can modulate mitotic activity of normal and malignant cells and exhibit genotoxic potential in some test-systems. It is assumed that metabolic conversion of phenolic groups in the catechol ring of epinephrine leads to the formation of reactive derivatives and superoxide anions capable of damaging cellular molecules including DNA. The aim of the present study was to evaluate the cytotoxic and genotoxic effects of epinephrine on human peripheral blood lymphocytes in vitro. The lowest concentration of epinephrine used in these experiments (5x10-10 M) was calculated to be in the range of the physiological blood level of epinephrine in humans. Three experimental concentrations corresponded to minimal (2x10-7 M), average (10-6 M) and maximal (5x10-6 M) therapeutic doses in human medicine. In addition, the highest concentrations exceeded the maximal therapeutic dose 10-fold (5x10-5 M) and 30-fold (1.5x10-4 M), respectively. On the basis of the results obtained it can be concluded that epinephrine had no influence on the appearance of chromosome aberrations under the described experimental conditions. However, mitotic index was significantly lower in cultures treated with the three highest concentrations of epinephrine used in this investigation.
AB  - Adrenalin može da promeni mitotsku aktivnost normalnih i malignih ćelija Pored toga, adrenalin ispoljava genotoksične efekte u nekim test-sistemima. Smatra se da metabolička konverzija fenolnih grupa u kateholnom prstenu adrenalina dovodi do stvaranja reaktivnih derivata i superoksidnih anjona sposobnih da oštete molekule u ćeliji, uključujući DNK. Cilj ovog istraživanja bio je da se izvrši evaluacija citotoksičnih i genotoksičnih efakata adrenalina na limfocite periferne krvi čoveka in vitro. Najniža eksperimentalna koncentracija adrenalina korišćena u ovom eksperimentu (5x10-10 M) proračunata je tako da bude uporedljiva sa fiziološkom koncentracijom adrenalina u krvi čoveka. Tri eksperimentalne koncentracije odgovaraju minimalnim (2x10-7 M), srednjim (10-6 M) i maksimalnim (5x10-6 M) terapijskim dozama u humanoj medicini. Pored toga, dve najviše koncentracije veće su od maksimalne terapijske doze deset (5x10-5 M) i trideset puta (1.5x10-4 M). Na osnovu dobijenih rezultata može se zaključiti da adrenalin nije značajno uticao na pojavu hromozomskih aberacija pod opisanim eksperimentalnim uslovima. Međutim, tri najviše koncentracije adrenalina upotrebljene u ovom eksperimentu dovode do značajnog smanjenja mitotske aktivnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cytogenetic analysis of the effects of epinephrine on cultured human lymphocytes
T1  - Citogenetička analiza dejstva adrenalina na humane limfocite u kulturi
VL  - 53
IS  - 2-3
SP  - 113
EP  - 120
DO  - 10.2298/AVB0303113D
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana J. and Potparević, Biljana and Marković, Biljana and Živković, Lada",
year = "2003",
abstract = "Epinephrine can modulate mitotic activity of normal and malignant cells and exhibit genotoxic potential in some test-systems. It is assumed that metabolic conversion of phenolic groups in the catechol ring of epinephrine leads to the formation of reactive derivatives and superoxide anions capable of damaging cellular molecules including DNA. The aim of the present study was to evaluate the cytotoxic and genotoxic effects of epinephrine on human peripheral blood lymphocytes in vitro. The lowest concentration of epinephrine used in these experiments (5x10-10 M) was calculated to be in the range of the physiological blood level of epinephrine in humans. Three experimental concentrations corresponded to minimal (2x10-7 M), average (10-6 M) and maximal (5x10-6 M) therapeutic doses in human medicine. In addition, the highest concentrations exceeded the maximal therapeutic dose 10-fold (5x10-5 M) and 30-fold (1.5x10-4 M), respectively. On the basis of the results obtained it can be concluded that epinephrine had no influence on the appearance of chromosome aberrations under the described experimental conditions. However, mitotic index was significantly lower in cultures treated with the three highest concentrations of epinephrine used in this investigation., Adrenalin može da promeni mitotsku aktivnost normalnih i malignih ćelija Pored toga, adrenalin ispoljava genotoksične efekte u nekim test-sistemima. Smatra se da metabolička konverzija fenolnih grupa u kateholnom prstenu adrenalina dovodi do stvaranja reaktivnih derivata i superoksidnih anjona sposobnih da oštete molekule u ćeliji, uključujući DNK. Cilj ovog istraživanja bio je da se izvrši evaluacija citotoksičnih i genotoksičnih efakata adrenalina na limfocite periferne krvi čoveka in vitro. Najniža eksperimentalna koncentracija adrenalina korišćena u ovom eksperimentu (5x10-10 M) proračunata je tako da bude uporedljiva sa fiziološkom koncentracijom adrenalina u krvi čoveka. Tri eksperimentalne koncentracije odgovaraju minimalnim (2x10-7 M), srednjim (10-6 M) i maksimalnim (5x10-6 M) terapijskim dozama u humanoj medicini. Pored toga, dve najviše koncentracije veće su od maksimalne terapijske doze deset (5x10-5 M) i trideset puta (1.5x10-4 M). Na osnovu dobijenih rezultata može se zaključiti da adrenalin nije značajno uticao na pojavu hromozomskih aberacija pod opisanim eksperimentalnim uslovima. Međutim, tri najviše koncentracije adrenalina upotrebljene u ovom eksperimentu dovode do značajnog smanjenja mitotske aktivnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cytogenetic analysis of the effects of epinephrine on cultured human lymphocytes, Citogenetička analiza dejstva adrenalina na humane limfocite u kulturi",
volume = "53",
number = "2-3",
pages = "113-120",
doi = "10.2298/AVB0303113D"
}

Đelić, N., Đelić, D. J., Potparević, B., Marković, B.,& Živković, L.. (2003). Cytogenetic analysis of the effects of epinephrine on cultured human lymphocytes. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(2-3), 113-120.
https://doi.org/10.2298/AVB0303113D

Đelić N, Đelić DJ, Potparević B, Marković B, Živković L. Cytogenetic analysis of the effects of epinephrine on cultured human lymphocytes. in Acta veterinaria. 2003;53(2-3):113-120.
doi:10.2298/AVB0303113D .

Đelić, Ninoslav, Đelić, Dijana J., Potparević, Biljana, Marković, Biljana, Živković, Lada, "Cytogenetic analysis of the effects of epinephrine on cultured human lymphocytes" in Acta veterinaria, 53, no. 2-3 (2003):113-120,
https://doi.org/10.2298/AVB0303113D . .