TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Đelić, Dijana J.
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Marković, Biljana
AU  - Lozance, Olivera
AU  - Blagojević, Miloš
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/901
AB  - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
VL  - 30
IS  - 4
SP  - 1144
EP  - 1149
DO  - 10.1590/S1415-47572007000600019
ER  - 

@article{
author = "Đelić, Ninoslav and Đelić, Dijana J. and Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozance, Olivera and Blagojević, Miloš",
year = "2007",
abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes",
volume = "30",
number = "4",
pages = "1144-1149",
doi = "10.1590/S1415-47572007000600019"
}

Đelić, N., Đelić, D. J., Potparević, B., Živković, L., Marković, B., Lozance, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149.
https://doi.org/10.1590/S1415-47572007000600019

Đelić N, Đelić DJ, Potparević B, Živković L, Marković B, Lozance O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149.
doi:10.1590/S1415-47572007000600019 .

Đelić, Ninoslav, Đelić, Dijana J., Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozance, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149,
https://doi.org/10.1590/S1415-47572007000600019 . .