TY  - JOUR
AU  - Jegorović, Boris
AU  - Nikolić, Aleksandra
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Šipetić Grujičić, Sandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4993
AB  - Background: The unpredictable course of Coronavirus
Disease 19 (COVID-19) is making good severity assessment tools crucial. This study aimed to assess the usefulness of serum amyloid A (SAA) and other acute-phase
reactants (APRs) in ambulatory care COVID-19 patients
and identified relationships between these markers and disease outcomes.
Methods: From August to November 2020, patients seen
in the outpatient department of the Clinic for Infectious and
Tropical Diseases (Belgrade, Serbia) with confirmed
COVID-19 were included. Patients were classified into
mild, moderate, and severe disease groups based on World
Health Organization criteria. SAA, C-reactive protein
(CRP), interleukin-6 (IL-6), procalcitonin (PCT), ferritin, fibrinogen, D-dimer, albumin, and transferrin were measured.
The median values of all APRs were compared between
COVID-19 severity groups, hospitalized and non-hospitalized patients, and survivors and non-survivors. The
Receiver operator characteristic (ROC) curve analysis was
used for the classification characteristics assessment of
individual APRs for the severity of illness, hospitalization,
and survival.
Results: Higher levels of SAA, CRP, IL-6, PCT, and lower
levels of transferrin and albumin were observed in severe
cases, hospitalized patients, and non-survivors. Based on
ROC curve analysis AUC for SAA has fair classification performance for disease severity (0.794) and death (0.732)
and good performance for hospitalization (0.853).
Conclusion: SAA is a valuable marker in everyday practice
for assessing COVID-19 severity and prognosis in ambulatory patients.
AB  - Uvod: Tok koronavirusne bolesti 2019 (COVID-19) je nepredvidiv, zbog čega su neophodni dobri alati za procenu težine ove infekcije. Svrha ovog istraživanja je procena značaja određivanja serumskog amiloida A (SAA) i drugih reaktanata akutne faze (RAF) kod ambulantnih pacijenata sa COVID-19, kao i identifikacija veze između ovih markera i ishoda bolesti. Metode: Od avgusta do novembra 2020. godine, u studju su uključeni pacijenti koji su pregledani u Prijemnoj ambulanti Klinike za infektivne i tropske bolesti (Beograd, Srbija), sa potvrđenim COVID-19. Pacijenti su bili klasifikovani u grupe blage, umerene i teške bolesti na osnovu kriterijuma Svetske zdravstvene organizacije. Određivane su vrednosti SAA, C-reaktivnog proteina (CRP), interleukina-6 (IL-6), prokalcitonina (PCT), feritina, fibrinogena, D-dimera, albumina i transferina. Medijane vrednosti RAF su poređene između grupa težine bolesti, bolesnika koji su bili hospitalizovani i onih koji nisu, kao i preživelih i preminulih. Korišćena je analiza Receiver operator characteristic (ROC) krive i Area-under-curve (AUC) za analizu klasifikacionih karakteristika pojedinačnih RAF za težinu bolesti, hospitalizaciju i preživljavanje. Rezultati: Viši nivoi SAA, CRP, IL-6, PCT i niži nivoi transferrina i albumina su uočeni kod osoba sa teškim oblikom bolesti, hospitalizovanih pacijenata i preminulih. Na osnovu analize ROC krive, AUC za SAA je pokazao zadovoljavajuće klasifikacione performanse za težinu bolesti (0,794) i smrtni ishod (0,732) i dobre performanse za hospitalizaciju (0,853). Zaključak: SAA je vredan marker u svakodnevnoj praksi za procenu težine i prognoze COVID-19 kod ambulantnih pacijenata.
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients
T1  - Značaj određivanja serumskog amiloida A i drugih reaktanata akutne faze kod ambulantnih pacijenata sa COVID-19
VL  - 42
IS  - 3
SP  - 492
EP  - 504
DO  - 10.5937/jomb0-42799
ER  - 

@article{
author = "Jegorović, Boris and Nikolić, Aleksandra and Milinković, Neda and Ignjatović, Svetlana and Šipetić Grujičić, Sandra",
year = "2023",
abstract = "Background: The unpredictable course of Coronavirus
Disease 19 (COVID-19) is making good severity assessment tools crucial. This study aimed to assess the usefulness of serum amyloid A (SAA) and other acute-phase
reactants (APRs) in ambulatory care COVID-19 patients
and identified relationships between these markers and disease outcomes.
Methods: From August to November 2020, patients seen
in the outpatient department of the Clinic for Infectious and
Tropical Diseases (Belgrade, Serbia) with confirmed
COVID-19 were included. Patients were classified into
mild, moderate, and severe disease groups based on World
Health Organization criteria. SAA, C-reactive protein
(CRP), interleukin-6 (IL-6), procalcitonin (PCT), ferritin, fibrinogen, D-dimer, albumin, and transferrin were measured.
The median values of all APRs were compared between
COVID-19 severity groups, hospitalized and non-hospitalized patients, and survivors and non-survivors. The
Receiver operator characteristic (ROC) curve analysis was
used for the classification characteristics assessment of
individual APRs for the severity of illness, hospitalization,
and survival.
Results: Higher levels of SAA, CRP, IL-6, PCT, and lower
levels of transferrin and albumin were observed in severe
cases, hospitalized patients, and non-survivors. Based on
ROC curve analysis AUC for SAA has fair classification performance for disease severity (0.794) and death (0.732)
and good performance for hospitalization (0.853).
Conclusion: SAA is a valuable marker in everyday practice
for assessing COVID-19 severity and prognosis in ambulatory patients., Uvod: Tok koronavirusne bolesti 2019 (COVID-19) je nepredvidiv, zbog čega su neophodni dobri alati za procenu težine ove infekcije. Svrha ovog istraživanja je procena značaja određivanja serumskog amiloida A (SAA) i drugih reaktanata akutne faze (RAF) kod ambulantnih pacijenata sa COVID-19, kao i identifikacija veze između ovih markera i ishoda bolesti. Metode: Od avgusta do novembra 2020. godine, u studju su uključeni pacijenti koji su pregledani u Prijemnoj ambulanti Klinike za infektivne i tropske bolesti (Beograd, Srbija), sa potvrđenim COVID-19. Pacijenti su bili klasifikovani u grupe blage, umerene i teške bolesti na osnovu kriterijuma Svetske zdravstvene organizacije. Određivane su vrednosti SAA, C-reaktivnog proteina (CRP), interleukina-6 (IL-6), prokalcitonina (PCT), feritina, fibrinogena, D-dimera, albumina i transferina. Medijane vrednosti RAF su poređene između grupa težine bolesti, bolesnika koji su bili hospitalizovani i onih koji nisu, kao i preživelih i preminulih. Korišćena je analiza Receiver operator characteristic (ROC) krive i Area-under-curve (AUC) za analizu klasifikacionih karakteristika pojedinačnih RAF za težinu bolesti, hospitalizaciju i preživljavanje. Rezultati: Viši nivoi SAA, CRP, IL-6, PCT i niži nivoi transferrina i albumina su uočeni kod osoba sa teškim oblikom bolesti, hospitalizovanih pacijenata i preminulih. Na osnovu analize ROC krive, AUC za SAA je pokazao zadovoljavajuće klasifikacione performanse za težinu bolesti (0,794) i smrtni ishod (0,732) i dobre performanse za hospitalizaciju (0,853). Zaključak: SAA je vredan marker u svakodnevnoj praksi za procenu težine i prognoze COVID-19 kod ambulantnih pacijenata.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients, Značaj određivanja serumskog amiloida A i drugih reaktanata akutne faze kod ambulantnih pacijenata sa COVID-19",
volume = "42",
number = "3",
pages = "492-504",
doi = "10.5937/jomb0-42799"
}

Jegorović, B., Nikolić, A., Milinković, N., Ignjatović, S.,& Šipetić Grujičić, S.. (2023). The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 42(3), 492-504.
https://doi.org/10.5937/jomb0-42799

Jegorović B, Nikolić A, Milinković N, Ignjatović S, Šipetić Grujičić S. The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients. in Journal of Medical Biochemistry. 2023;42(3):492-504.
doi:10.5937/jomb0-42799 .

Jegorović, Boris, Nikolić, Aleksandra, Milinković, Neda, Ignjatović, Svetlana, Šipetić Grujičić, Sandra, "The utility of serum amyloid a and other acute-phase reactants determination in ambulatory care COVID-19 patients" in Journal of Medical Biochemistry, 42, no. 3 (2023):492-504,
https://doi.org/10.5937/jomb0-42799 . .

TY  - CONF
AU  - Nikolić, Aleksandra
AU  - Popović, Katarina
AU  - Milenković, Kristijan
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4812
AB  - Уочено је да развоју компликација у трудноћи попут гестацијске хипертензије, прееклампсије, гестацијског дијабетеса и интраутериног застоја у расту плода, претходи појава интензивног оксидативног стреса код труднице.
AB  - The development of pregnancy complications (gestational hypertension, preeclampsia, gestational diabetes, and intrauterine fetal growth restriction) was shown to be preceded by pronounced oxidative stress in pregnant women.
T1  - Оксидативно-стресни статус код жена са високоризичном трудноћом са и без компликација
T1  - Oxidative stress status in women with high-risk pregnancies with and without complications
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4812
ER  - 

@conference{
author = "Nikolić, Aleksandra and Popović, Katarina and Milenković, Kristijan",
year = "2023",
abstract = "Уочено је да развоју компликација у трудноћи попут гестацијске хипертензије, прееклампсије, гестацијског дијабетеса и интраутериног застоја у расту плода, претходи појава интензивног оксидативног стреса код труднице., The development of pregnancy complications (gestational hypertension, preeclampsia, gestational diabetes, and intrauterine fetal growth restriction) was shown to be preceded by pronounced oxidative stress in pregnant women.",
title = "Оксидативно-стресни статус код жена са високоризичном трудноћом са и без компликација, Oxidative stress status in women with high-risk pregnancies with and without complications",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4812"
}

Nikolić, A., Popović, K.,& Milenković, K.. (2023). Оксидативно-стресни статус код жена са високоризичном трудноћом са и без компликација. .
https://hdl.handle.net/21.15107/rcub_farfar_4812

Nikolić A, Popović K, Milenković K. Оксидативно-стресни статус код жена са високоризичном трудноћом са и без компликација. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_4812 .

Nikolić, Aleksandra, Popović, Katarina, Milenković, Kristijan, "Оксидативно-стресни статус код жена са високоризичном трудноћом са и без компликација" (2023),
https://hdl.handle.net/21.15107/rcub_farfar_4812 .

TY  - JOUR
AU  - Topić, Aleksandra
AU  - Francuski, Đorđe
AU  - Nikolić, Aleksandra
AU  - Milošević, Katarina
AU  - Jovičić, Snežana
AU  - Marković, Bojan
AU  - Đukić, Mirjana
AU  - Radojković, Dragica
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2833
AB  - Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Fetal and Pediatric Pathology
T1  - The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma
VL  - 36
IS  - 4
SP  - 294
EP  - 303
DO  - 10.1080/15513815.2017.1315199
ER  - 

@article{
author = "Topić, Aleksandra and Francuski, Đorđe and Nikolić, Aleksandra and Milošević, Katarina and Jovičić, Snežana and Marković, Bojan and Đukić, Mirjana and Radojković, Dragica",
year = "2017",
abstract = "Introduction: The significance of oxidative stress in pathogenesis of childhood asthma was recognized, but its role in the clinical manifestations of disease is still unclear. Materials and Methods: The study was conducted in 96 asthmatic children. The urinary biomarker of oxidative stress, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG/creatinine) was determined by using HPLC-MS/MS. ELISA was performed to measure myeloperoxidase (MPO) and Cu,Zn-superoxide dismutase (Cu, Zn-SOD) in serum. Results: Logistic regression analysis revealed that female gender, tobacco smoke exposure, and increased 8-oxodG/creatinine were associated with risk for intermittent asthma, while the positive allergy test and increased Cu, Zn-SOD were associated with eczema in asthmatic children. Higher MPO (p = 0.033), and percent of granulocytes (p = 0.030) were found in severe persistent asthma in comparison to intermittent or mild persistent asthma. Conclusion: The main findings that TSE-induced oxidative stress is a risk for intermittent asthma and eczema may be clinically significant for the disease prevention and therapeutic improvements.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Fetal and Pediatric Pathology",
title = "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma",
volume = "36",
number = "4",
pages = "294-303",
doi = "10.1080/15513815.2017.1315199"
}

Topić, A., Francuski, Đ., Nikolić, A., Milošević, K., Jovičić, S., Marković, B., Đukić, M.,& Radojković, D.. (2017). The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology
Taylor & Francis Inc, Philadelphia., 36(4), 294-303.
https://doi.org/10.1080/15513815.2017.1315199

Topić A, Francuski Đ, Nikolić A, Milošević K, Jovičić S, Marković B, Đukić M, Radojković D. The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma. in Fetal and Pediatric Pathology. 2017;36(4):294-303.
doi:10.1080/15513815.2017.1315199 .

Topić, Aleksandra, Francuski, Đorđe, Nikolić, Aleksandra, Milošević, Katarina, Jovičić, Snežana, Marković, Bojan, Đukić, Mirjana, Radojković, Dragica, "The Role of Oxidative Stress in the Clinical Manifestations of Childhood Asthma" in Fetal and Pediatric Pathology, 36, no. 4 (2017):294-303,
https://doi.org/10.1080/15513815.2017.1315199 . .

TY  - JOUR
AU  - Petrović-Stanojević, Nataša
AU  - Topić, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Stankovic, Marija
AU  - Dopuđa-Pantić, Vesna
AU  - Milenković, Branislava
AU  - Radojković, Dragica
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2142
AB  - Background and Objectives Polymorphisms of beta2-adrenergic receptor gene (ADRB2) are clinically relevant for several reasons, including as a risk factor for asthma development/severity and predicting the effectiveness of treatment with beta2-agonists in reducing asthma symptoms. The aim of this study was to examine the association between ADRB2 gene polymorphisms and asthma in the Serbian population, and to evaluate the therapeutic response in relation to the ADRB2 genotype. Methods The study included 171 patients with asthma and 101 healthy subjects as the control group. Genotyping of Arg16Gly and Gln27Glu polymorphisms was performed by direct sequencing of polymerase chain reaction (PCR) products. Results In Serbian adults, carriers of the 27Gln allele and 27Gln/Gln genotype were at higher risk of asthma [odds ratio (OR) 2.5, 95 % confidence interval (CI) 1.6-3.8, and OR 3.00, 95 % CI 1.7-5.3, respectively], while the presence of the 27Glu allele and 27Gln/Glu genotype were found to be protective of asthma (OR 0.4, 95 % CI 0.3-0.6, and OR 0.3, 95 % CI 0.1-0.7, respectively). Furthermore, we found that the presence of the 27Gln allele in asthmatics younger than 50 years leads to a better response to therapy with long-acting beta2-agonists (LABA) in combination with prevailing low and moderate doses of inhaled corticosteroids (ICS), while carriers of the 27Glu allele over 50 years old are more likely to respond to LABA + ICS therapy. Conclusion We identified that in Serbian adults the 27Gln allele and 27Gln homozygosity are risk factors for asthma, which may be of clinical interest in disease prevention. The finding that younger carriers of the 27Gln allele respond better to LABA + ICS therapy may be utilized in personalized asthma treatment.
PB  - Adis Int Ltd, Northcote
T2  - Molecular Diagnosis & Therapy
T1  - Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists
VL  - 18
IS  - 6
SP  - 639
EP  - 646
DO  - 10.1007/s40291-014-0116-1
ER  - 

@article{
author = "Petrović-Stanojević, Nataša and Topić, Aleksandra and Nikolić, Aleksandra and Stankovic, Marija and Dopuđa-Pantić, Vesna and Milenković, Branislava and Radojković, Dragica",
year = "2014",
abstract = "Background and Objectives Polymorphisms of beta2-adrenergic receptor gene (ADRB2) are clinically relevant for several reasons, including as a risk factor for asthma development/severity and predicting the effectiveness of treatment with beta2-agonists in reducing asthma symptoms. The aim of this study was to examine the association between ADRB2 gene polymorphisms and asthma in the Serbian population, and to evaluate the therapeutic response in relation to the ADRB2 genotype. Methods The study included 171 patients with asthma and 101 healthy subjects as the control group. Genotyping of Arg16Gly and Gln27Glu polymorphisms was performed by direct sequencing of polymerase chain reaction (PCR) products. Results In Serbian adults, carriers of the 27Gln allele and 27Gln/Gln genotype were at higher risk of asthma [odds ratio (OR) 2.5, 95 % confidence interval (CI) 1.6-3.8, and OR 3.00, 95 % CI 1.7-5.3, respectively], while the presence of the 27Glu allele and 27Gln/Glu genotype were found to be protective of asthma (OR 0.4, 95 % CI 0.3-0.6, and OR 0.3, 95 % CI 0.1-0.7, respectively). Furthermore, we found that the presence of the 27Gln allele in asthmatics younger than 50 years leads to a better response to therapy with long-acting beta2-agonists (LABA) in combination with prevailing low and moderate doses of inhaled corticosteroids (ICS), while carriers of the 27Glu allele over 50 years old are more likely to respond to LABA + ICS therapy. Conclusion We identified that in Serbian adults the 27Gln allele and 27Gln homozygosity are risk factors for asthma, which may be of clinical interest in disease prevention. The finding that younger carriers of the 27Gln allele respond better to LABA + ICS therapy may be utilized in personalized asthma treatment.",
publisher = "Adis Int Ltd, Northcote",
journal = "Molecular Diagnosis & Therapy",
title = "Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists",
volume = "18",
number = "6",
pages = "639-646",
doi = "10.1007/s40291-014-0116-1"
}

Petrović-Stanojević, N., Topić, A., Nikolić, A., Stankovic, M., Dopuđa-Pantić, V., Milenković, B.,& Radojković, D.. (2014). Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists. in Molecular Diagnosis & Therapy
Adis Int Ltd, Northcote., 18(6), 639-646.
https://doi.org/10.1007/s40291-014-0116-1

Petrović-Stanojević N, Topić A, Nikolić A, Stankovic M, Dopuđa-Pantić V, Milenković B, Radojković D. Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists. in Molecular Diagnosis & Therapy. 2014;18(6):639-646.
doi:10.1007/s40291-014-0116-1 .

Petrović-Stanojević, Nataša, Topić, Aleksandra, Nikolić, Aleksandra, Stankovic, Marija, Dopuđa-Pantić, Vesna, Milenković, Branislava, Radojković, Dragica, "Polymorphisms of Beta2-Adrenergic Receptor Gene in Serbian Asthmatic Adults: Effects on Response to Beta-Agonists" in Molecular Diagnosis & Therapy, 18, no. 6 (2014):639-646,
https://doi.org/10.1007/s40291-014-0116-1 . .

TY  - JOUR
AU  - Topić, Aleksandra
AU  - Ljujić, Mila
AU  - Nikolić, Aleksandra
AU  - Petrović-Stanojević, Nataša
AU  - Dopuđa-Pantić, Vesna
AU  - Mitić-Milikić, Marija
AU  - Radojković, Dragica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1547
AB  - Imbalance between neutrophil elastase and alpha-1-antitrypsin (AAT) leads to emphysema in smokers as well as in patients with inherited alpha-1-antitrypsin deficiency. AAT as a proven inhibitor of apoptosis may play role in lung cancer (LC) progression. The aim was to analyse AAT protein variants and polymorphism in promoter region of the neutrophil elastase gene (ELA2) in patients with primary lung cancer. AAT phenotypisation by isoelectric focusing method and ELA2 gene promoter characterization by DNA sequencing were performed in 66 patients with primary lung cancer. Results showed that the frequency of M1 allele and PiM1 homozygotes in LC patients was significantly higher when compared to the healthy subjects (f = 0.6360 and 0.7424 respectively). The most frequent ELA2 promoter region genotypes in LC patients were -903TT and -741GG. There were significantly more patients with intermediate and high ELA2 genotype activity, compared to those with low activity (91% vs. 9%, respectively). In conclusion, we found that PiM1 homozygosity could be associated with the lung cancer, probably due to increased synthesis of this antiapoptotic protein. Non-MM variants of AAT and ELA2 genotypes with predicted intermediate or high activity could also represent a risk factor for aggressive form of lung cancer associated with extrathoracic metastases.
PB  - Springer, Dordrecht
T2  - Pathology & Oncology Research
T1  - Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer
VL  - 17
IS  - 1
SP  - 75
EP  - 80
DO  - 10.1007/s12253-010-9283-5
ER  - 

@article{
author = "Topić, Aleksandra and Ljujić, Mila and Nikolić, Aleksandra and Petrović-Stanojević, Nataša and Dopuđa-Pantić, Vesna and Mitić-Milikić, Marija and Radojković, Dragica",
year = "2011",
abstract = "Imbalance between neutrophil elastase and alpha-1-antitrypsin (AAT) leads to emphysema in smokers as well as in patients with inherited alpha-1-antitrypsin deficiency. AAT as a proven inhibitor of apoptosis may play role in lung cancer (LC) progression. The aim was to analyse AAT protein variants and polymorphism in promoter region of the neutrophil elastase gene (ELA2) in patients with primary lung cancer. AAT phenotypisation by isoelectric focusing method and ELA2 gene promoter characterization by DNA sequencing were performed in 66 patients with primary lung cancer. Results showed that the frequency of M1 allele and PiM1 homozygotes in LC patients was significantly higher when compared to the healthy subjects (f = 0.6360 and 0.7424 respectively). The most frequent ELA2 promoter region genotypes in LC patients were -903TT and -741GG. There were significantly more patients with intermediate and high ELA2 genotype activity, compared to those with low activity (91% vs. 9%, respectively). In conclusion, we found that PiM1 homozygosity could be associated with the lung cancer, probably due to increased synthesis of this antiapoptotic protein. Non-MM variants of AAT and ELA2 genotypes with predicted intermediate or high activity could also represent a risk factor for aggressive form of lung cancer associated with extrathoracic metastases.",
publisher = "Springer, Dordrecht",
journal = "Pathology & Oncology Research",
title = "Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer",
volume = "17",
number = "1",
pages = "75-80",
doi = "10.1007/s12253-010-9283-5"
}

Topić, A., Ljujić, M., Nikolić, A., Petrović-Stanojević, N., Dopuđa-Pantić, V., Mitić-Milikić, M.,& Radojković, D.. (2011). Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer. in Pathology & Oncology Research
Springer, Dordrecht., 17(1), 75-80.
https://doi.org/10.1007/s12253-010-9283-5

Topić A, Ljujić M, Nikolić A, Petrović-Stanojević N, Dopuđa-Pantić V, Mitić-Milikić M, Radojković D. Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer. in Pathology & Oncology Research. 2011;17(1):75-80.
doi:10.1007/s12253-010-9283-5 .

Topić, Aleksandra, Ljujić, Mila, Nikolić, Aleksandra, Petrović-Stanojević, Nataša, Dopuđa-Pantić, Vesna, Mitić-Milikić, Marija, Radojković, Dragica, "Alpha-1-antitrypsin Phenotypes and Neutrophil Elastase Gene Promoter Polymorphisms in Lung Cancer" in Pathology & Oncology Research, 17, no. 1 (2011):75-80,
https://doi.org/10.1007/s12253-010-9283-5 . .

TY  - JOUR
AU  - Ljujić, Mila
AU  - Topić, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Divac, Aleksandra
AU  - Grujić, Milan
AU  - Mitić-Milikić, Marija
AU  - Radojković, Dragica
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1383
AB  - The alpha-1-antitrypsin (A1AT) gene is highly polymorphic, with more than 100 genetic variants identified of which some can affect A1AT protein concentration and/or function and lead to pulmonary and/or liver disease. This study reports on the characterization of a p.G320R variant found in two patients, one with emphysema and the other with lung cancer. This variant results from a single base-pair substitution in exon 4 of the A1AT gene, and has been characterized as P by isoelectric focusing. Functional evaluation of the A1AT p.G320R variant was through comparing specific trypsin inhibitory activity in two patients with pulmonary disorders, carriers of the p.G320R variant, and 19 healthy individuals, carriers of normal A1AT M variants. Results showed that specific trypsin inhibitory activity was lower in both emphysema (2.45 mU/g) and lung cancer (2.07 mU/g) patients than in carriers of the normal variants (range 2.51-3.71 mU/g). This rare A1AT variant is associated with reduced functional activity of A1AT protein. Considering that it was found in patients with severe pulmonary disorders, this variant could be of clinical significance.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics and Molecular Biology
T1  - Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients
VL  - 33
IS  - 1
SP  - 5
EP  - 8
DO  - 10.1590/S1415-47572009005000100
ER  - 

@article{
author = "Ljujić, Mila and Topić, Aleksandra and Nikolić, Aleksandra and Divac, Aleksandra and Grujić, Milan and Mitić-Milikić, Marija and Radojković, Dragica",
year = "2010",
abstract = "The alpha-1-antitrypsin (A1AT) gene is highly polymorphic, with more than 100 genetic variants identified of which some can affect A1AT protein concentration and/or function and lead to pulmonary and/or liver disease. This study reports on the characterization of a p.G320R variant found in two patients, one with emphysema and the other with lung cancer. This variant results from a single base-pair substitution in exon 4 of the A1AT gene, and has been characterized as P by isoelectric focusing. Functional evaluation of the A1AT p.G320R variant was through comparing specific trypsin inhibitory activity in two patients with pulmonary disorders, carriers of the p.G320R variant, and 19 healthy individuals, carriers of normal A1AT M variants. Results showed that specific trypsin inhibitory activity was lower in both emphysema (2.45 mU/g) and lung cancer (2.07 mU/g) patients than in carriers of the normal variants (range 2.51-3.71 mU/g). This rare A1AT variant is associated with reduced functional activity of A1AT protein. Considering that it was found in patients with severe pulmonary disorders, this variant could be of clinical significance.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics and Molecular Biology",
title = "Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients",
volume = "33",
number = "1",
pages = "5-8",
doi = "10.1590/S1415-47572009005000100"
}

Ljujić, M., Topić, A., Nikolić, A., Divac, A., Grujić, M., Mitić-Milikić, M.,& Radojković, D.. (2010). Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients. in Genetics and Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 33(1), 5-8.
https://doi.org/10.1590/S1415-47572009005000100

Ljujić M, Topić A, Nikolić A, Divac A, Grujić M, Mitić-Milikić M, Radojković D. Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients. in Genetics and Molecular Biology. 2010;33(1):5-8.
doi:10.1590/S1415-47572009005000100 .

Ljujić, Mila, Topić, Aleksandra, Nikolić, Aleksandra, Divac, Aleksandra, Grujić, Milan, Mitić-Milikić, Marija, Radojković, Dragica, "Identification of a rare p.G320R alpha-1-antitrypsin variant in emphysema and lung cancer patients" in Genetics and Molecular Biology, 33, no. 1 (2010):5-8,
https://doi.org/10.1590/S1415-47572009005000100 . .