@conference{
author = "Račić, Anđelka and Čalija, Bojan and Dobričić, Vladimir and Jurišić Dukovsk, Bisera and Lovrić, Jasmina and Krajišnik, Danina",
year = "2022",
abstract = "To improve ocular bioavailability of eye drops, viscosity-increasing polymers with improved
functional properties have been used (1,2). The aim of this study was formulation and evaluation of
viscous eye drops using vehicles containing hydroxypropyl guar gum (HPG) (0.25% w/v), sodium
hyaluronate (SH) (0.4% w/v), and their combination (HPG-SH) as carriers for olopatadine (0.1% w/v).
Physicochemical properties (appearance, clarity, pH, osmolality, viscosity) of the prepared
formulations were tested. The drug permeability was estimated in vitro using HCE-T cell-based models
and the parallel artificial membrane permeability assay (PAMPA). MTT cytotoxicity assay was
performed at the end of the permeability study. Physicochemical stability of the formulated eye drops
was tested during 12 months. An accelerated stability study was performed using the heating/cooling
cycles test. The clarity, pH and osmolality of all formulations were in acceptable range for ophthalmic
preparations. Formulation HPG-SH showed significantly higher viscosity (73.1 mPa·s) than
formulations with single polymer (7.4 mPa·s for HPG i.e 3.7 mPa·s for SH) pointing to synergistic effect
of polymers. The addition of polymers led to a decrease in transcorneal permeability and lower
permeability coefficients with prolonged residence of drug at the ocular surface. The results of MTT
assay demonstrated that the tested formulations were biocompatible and well tolerated. Formulated
eye drops showed satisfactory physicochemical stability under all storage conditions.
Olopatadine was successfully formulated in biocompatible viscous ophthalmic solutions. The
combination of viscosity enhancer HPG with mucoadhesive polymer SH has a potential to improve
precorneal residence time and therapeutic effect of olopatadine., Polimeri poboljšanih funkcionalnih karakteristika koji povećavaju viskozitet koriste se u
kapima za oči kako bi se povećala okularna raspoloživost (1,2). Cilj ove studije bio je formulacija i
ispitivanje viskoznih kapi za oči korišćenjem vehikuluma koji sadrže hidroksipropil guar gumu (HPG)
(0,25% v/v), natrijum hijaluronat (SH) (0,4% v/v) i njihovu kombinaciju (HPG-SH) kao nosača za
olopatadin (0,1% v/v). Ispitivane su fizičkohemijske osobine (izgled, bistrina, pH, osmolalnost,
viskozitet) pripremljenih formulacija. Permeabilnost leka je procenjena in vitro korišćenjem modela
zasnovanih na HCE-T ćelijama i testa permeabilnosti na paralelnim veštačkim membranama (PAMPA).
MTT citotoksični test sproveden je na kraju studije permeabilnosti. Fizičkohemijska stabilnost
formulisanih kapi za oči ispitivana je tokom 12 meseci. Sprovedena je ubrzana studija stabilnosti
korišćenjem cikličnog testa grejanja/hlađenja. Bistrina, pH i osmolalnost svih formulacija bili su u
prihvatljivom opsegu za oftalmološke preparate. Formulacija HPG-SH je pokazala značajno viši
viskozitet (73,1 mPa·s) od formulacija sa pojedinačnim polimerom (7,4 mPa·s za HPG, tj. 3,7 mPa·s za
SH) ukazujući na sinergistički efekat polimera. Dodatak polimera je doveo do smanjenja
transkornealne permeabilnosti i nižih koeficijenata permeabilnosti uz produženo zadržavanje leka na
površini oka. Rezultati MTT testa su pokazali da su testirane formulacije biokompatibilne i dobro
podnošljive. Formulisane kapi za oči imale su zadovoljavajuću fizičkohemijsku stabilnost pri svim
uslovima čuvanja. Uspešno su formulisani biokompatibilni viskozni rastvori za oftalmološku primenu
sa olopatadinom. Kombinacija HPG kao sredstva za povećanje viskoziteta sa mukoadhezivnim
polimerom SH ima potencijal da poboljša prekornealno vreme zadržavanja i terapijski efekat
olopatadina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Evaluation of the influence of hydroxypropylguar gum and hyaluronic acid on physicochemical and functional characteristics of ophthalmic vehicles for olopatadine, Procena uticaja hidroksipropil guar gume i hijaluronske kiseline na fizičkohemijske i funkcionalne karakteristike oftalmoloških vehikuluma za olopatadin",
volume = "72",
number = "4 suplement",
pages = "S388-S389",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4566"
}
