TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Fahr, Alfred
AU  - Maibach, Howard
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3319
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3438
AB  - Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements
VL  - 45
IS  - 1
SP  - 43
EP  - 54
DO  - 10.1080/03639045.2018.1515220
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Fahr, Alfred and Maibach, Howard",
year = "2019",
abstract = "Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements",
volume = "45",
number = "1",
pages = "43-54",
doi = "10.1080/03639045.2018.1515220"
}

Dragičević, N., Krajišnik, D., Milić, J., Fahr, A.,& Maibach, H.. (2019). Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 45(1), 43-54.
https://doi.org/10.1080/03639045.2018.1515220

Dragičević N, Krajišnik D, Milić J, Fahr A, Maibach H. Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy. 2019;45(1):43-54.
doi:10.1080/03639045.2018.1515220 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Fahr, Alfred, Maibach, Howard, "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements" in Drug Development and Industrial Pharmacy, 45, no. 1 (2019):43-54,
https://doi.org/10.1080/03639045.2018.1515220 . .

TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Fahr, Alfred
AU  - Maibach, Howard
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3319
AB  - Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements
VL  - 45
IS  - 1
SP  - 43
EP  - 54
DO  - 10.1080/03639045.2018.1515220
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Fahr, Alfred and Maibach, Howard",
year = "2019",
abstract = "Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements",
volume = "45",
number = "1",
pages = "43-54",
doi = "10.1080/03639045.2018.1515220"
}

Dragičević, N., Krajišnik, D., Milić, J., Fahr, A.,& Maibach, H.. (2019). Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 45(1), 43-54.
https://doi.org/10.1080/03639045.2018.1515220

Dragičević N, Krajišnik D, Milić J, Fahr A, Maibach H. Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy. 2019;45(1):43-54.
doi:10.1080/03639045.2018.1515220 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Fahr, Alfred, Maibach, Howard, "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements" in Drug Development and Industrial Pharmacy, 45, no. 1 (2019):43-54,
https://doi.org/10.1080/03639045.2018.1515220 . .

TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Pecarski, D
AU  - Jugović, Z
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3283
AB  - The aim of this study was to develop a semisolid formulation containing liposomes loaded with coenzyme Q10 (Q10). Q10-loaded liposome dispersion prepared from non-hydrogenated lecithin and characterized for particle size, polydispersity index (PDI), pH value and Q10-content was incorporated into carbomer gel, and a liposome gel was obtained. Liposome gel and liposome-free gel were analyzed for flow properties by continuous rheology measurements, pH values and Q10-content, 48 h after preparation and after a temperature stress test (1 cycle: 24 h at 4 o C, 24 h at 20±2 o C and 24 h at 40 o C), in order to predict their long-term stability. Liposomes were identified in liposome dispersion and liposome gel by freeze fracture electron microscopy (FFEM), while their particle size, PDI and zeta potential were determined by photon correlation spectroscopy (PCS). Q10-loaded liposomes were of small particle size (125 nm), homogeneous (PDI=0.2) and negatively charged, and their incorporation into the gel did not significantly change (p&0.05) their particle size and PDI. FFEM confirmed liposomes presence in the liposome gel. Liposome and liposome-free gel revealed non-Newtonian, shear-thinning plastic flow behavior. The temperature stress test revealed that temperature changes did not significantly influence (p&0.05) the pH value, while they significantly decreased (p&0.05) Q10-content in gels. Q10 was significantly more stable (p&0.05) in liposome gel than in liposome-free gel. Rheological parameters of liposome-free gel significantly changed, in contrast to the liposome gel. In conclusion, Q10-loaded liposome gel suitable for dermal use was developed, exhibiting high stability even after subjecting to the temperature stress test.
PB  - Bulgarian Academy of Sciences
T2  - Bulgarian Chemical Communications
T1  - Hydrophilic gel containing coenzyme Q 10 -loaded liposomes: Preparation, characterization and stress stability tests
VL  - 51
IS  - 1
SP  - 117
EP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3283
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Pecarski, D and Jugović, Z",
year = "2019",
abstract = "The aim of this study was to develop a semisolid formulation containing liposomes loaded with coenzyme Q10 (Q10). Q10-loaded liposome dispersion prepared from non-hydrogenated lecithin and characterized for particle size, polydispersity index (PDI), pH value and Q10-content was incorporated into carbomer gel, and a liposome gel was obtained. Liposome gel and liposome-free gel were analyzed for flow properties by continuous rheology measurements, pH values and Q10-content, 48 h after preparation and after a temperature stress test (1 cycle: 24 h at 4 o C, 24 h at 20±2 o C and 24 h at 40 o C), in order to predict their long-term stability. Liposomes were identified in liposome dispersion and liposome gel by freeze fracture electron microscopy (FFEM), while their particle size, PDI and zeta potential were determined by photon correlation spectroscopy (PCS). Q10-loaded liposomes were of small particle size (125 nm), homogeneous (PDI=0.2) and negatively charged, and their incorporation into the gel did not significantly change (p&0.05) their particle size and PDI. FFEM confirmed liposomes presence in the liposome gel. Liposome and liposome-free gel revealed non-Newtonian, shear-thinning plastic flow behavior. The temperature stress test revealed that temperature changes did not significantly influence (p&0.05) the pH value, while they significantly decreased (p&0.05) Q10-content in gels. Q10 was significantly more stable (p&0.05) in liposome gel than in liposome-free gel. Rheological parameters of liposome-free gel significantly changed, in contrast to the liposome gel. In conclusion, Q10-loaded liposome gel suitable for dermal use was developed, exhibiting high stability even after subjecting to the temperature stress test.",
publisher = "Bulgarian Academy of Sciences",
journal = "Bulgarian Chemical Communications",
title = "Hydrophilic gel containing coenzyme Q 10 -loaded liposomes: Preparation, characterization and stress stability tests",
volume = "51",
number = "1",
pages = "117-124",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3283"
}

Dragičević, N., Krajišnik, D., Milić, J., Pecarski, D.,& Jugović, Z.. (2019). Hydrophilic gel containing coenzyme Q 10 -loaded liposomes: Preparation, characterization and stress stability tests. in Bulgarian Chemical Communications
Bulgarian Academy of Sciences., 51(1), 117-124.
https://hdl.handle.net/21.15107/rcub_farfar_3283

Dragičević N, Krajišnik D, Milić J, Pecarski D, Jugović Z. Hydrophilic gel containing coenzyme Q 10 -loaded liposomes: Preparation, characterization and stress stability tests. in Bulgarian Chemical Communications. 2019;51(1):117-124.
https://hdl.handle.net/21.15107/rcub_farfar_3283 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Pecarski, D, Jugović, Z, "Hydrophilic gel containing coenzyme Q 10 -loaded liposomes: Preparation, characterization and stress stability tests" in Bulgarian Chemical Communications, 51, no. 1 (2019):117-124,
https://hdl.handle.net/21.15107/rcub_farfar_3283 .

TY  - CHAP
AU  - Pantelić, Ivana
AU  - Milić, Jela
AU  - Vuleta, Gordana
AU  - Dragičević, Nina
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2506
AB  - Due to the unceasing progress in design of novel emulsifiers, powerful influence of the ongoing ‘green’ trends in cosmetic industry, but also global environmental concerns, there is an increasing demand in development and application of natural-origin, biodegradable and skin-friendly emulsifiers. The present work aims to provide a deeper insight into the group of alkyl polyglucoside emulsifiers and their prospective impact on drug percutaneous permeation. Complexity of the observed lamellar liquid crystal and lamellar gel phases, which represent the basic mechanism of physical stabilisation by the alkyl polyglucosides, entails a comprehensive physicochemical evaluation of any alkyl polyglucoside-based prospective vehicle, prior to assessing its impact on skin absorption. Research conducted so far has suggested several possible mechanisms of alkyl polyglucoside-mediated percutaneous penetration enhancement, which are carefully elaborated throughout the chapter: (1) the observed similarity between the aforementioned complex structures which dominate alkyl polyglucoside-stabilised systems and the inherent stratum corneum lipid ordering could enhance penetration due to the optimal balance between their order and fluidity, (2) the observed complex colloidal structure may lead to diverse modes of water distribution within these systems, providing sustained skin hydration and hence improved skin permeability, and/or (3) the impact on a drug’s thermodynamic activity, easily tailored by the addition of various co-solvents/potential penetration enhancers.
PB  - Springer Berlin Heidelberg
T2  - Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the
T1  - Natural emulsifiers of the alkyl polyglucoside type and their influence on the permeation of drugs
SP  - 231
EP  - 250
DO  - 10.1007/978-3-662-47039-8_14
ER  - 

@inbook{
author = "Pantelić, Ivana and Milić, Jela and Vuleta, Gordana and Dragičević, Nina and Savić, Snežana",
year = "2015",
abstract = "Due to the unceasing progress in design of novel emulsifiers, powerful influence of the ongoing ‘green’ trends in cosmetic industry, but also global environmental concerns, there is an increasing demand in development and application of natural-origin, biodegradable and skin-friendly emulsifiers. The present work aims to provide a deeper insight into the group of alkyl polyglucoside emulsifiers and their prospective impact on drug percutaneous permeation. Complexity of the observed lamellar liquid crystal and lamellar gel phases, which represent the basic mechanism of physical stabilisation by the alkyl polyglucosides, entails a comprehensive physicochemical evaluation of any alkyl polyglucoside-based prospective vehicle, prior to assessing its impact on skin absorption. Research conducted so far has suggested several possible mechanisms of alkyl polyglucoside-mediated percutaneous penetration enhancement, which are carefully elaborated throughout the chapter: (1) the observed similarity between the aforementioned complex structures which dominate alkyl polyglucoside-stabilised systems and the inherent stratum corneum lipid ordering could enhance penetration due to the optimal balance between their order and fluidity, (2) the observed complex colloidal structure may lead to diverse modes of water distribution within these systems, providing sustained skin hydration and hence improved skin permeability, and/or (3) the impact on a drug’s thermodynamic activity, easily tailored by the addition of various co-solvents/potential penetration enhancers.",
publisher = "Springer Berlin Heidelberg",
journal = "Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the",
booktitle = "Natural emulsifiers of the alkyl polyglucoside type and their influence on the permeation of drugs",
pages = "231-250",
doi = "10.1007/978-3-662-47039-8_14"
}

Pantelić, I., Milić, J., Vuleta, G., Dragičević, N.,& Savić, S.. (2015). Natural emulsifiers of the alkyl polyglucoside type and their influence on the permeation of drugs. in Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the
Springer Berlin Heidelberg., 231-250.
https://doi.org/10.1007/978-3-662-47039-8_14

Pantelić I, Milić J, Vuleta G, Dragičević N, Savić S. Natural emulsifiers of the alkyl polyglucoside type and their influence on the permeation of drugs. in Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the. 2015;:231-250.
doi:10.1007/978-3-662-47039-8_14 .

Pantelić, Ivana, Milić, Jela, Vuleta, Gordana, Dragičević, Nina, Savić, Snežana, "Natural emulsifiers of the alkyl polyglucoside type and their influence on the permeation of drugs" in Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement: Modification of the (2015):231-250,
https://doi.org/10.1007/978-3-662-47039-8_14 . .