TY  - JOUR
AU  - Janićijević, Željko
AU  - Stanković, Ana
AU  - Žegura, Bojana
AU  - Veljović, Đorđe
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Filipič, Metka
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3947
AB  - We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.
PB  - Springer Science and Business Media B.V.
T2  - Journal of Nanoparticle Research
T1  - Safe-by-design gelatin-modified zinc oxide nanoparticles
VL  - 23
IS  - 9
DO  - 10.1007/s11051-021-05312-3
ER  - 

@article{
author = "Janićijević, Željko and Stanković, Ana and Žegura, Bojana and Veljović, Đorđe and Đekić, Ljiljana and Krajišnik, Danina and Filipič, Metka and Stevanović, Magdalena",
year = "2021",
abstract = "We report an innovative low-cost wet  precipitation  synthesis  method  for  gelatin-modified   zinc oxide nanoparticles (GM ZnO NPs) at the inter- face  between  the  gelatin  hydrogel  and  aqueous  elec- trolyte.  Diffusion  of  ammonia  through  the  hydrogel   matrices with different gelatin contents induced pre- cipitation  of  the  product  in  contact  with  the  surface   of  the  aqueous  solution  of  zinc  ions.  The  obtained   precipitate  was  subjected  to  thermal  treatment  to  partially  decompose  the  adsorbed  gelatin  in  the  NP   structure. Physicochemical properties of obtained  GM  ZnO  NPs  were  characterized  by  X-ray  powder   diffraction (XRD), scanning electron microscopy  (SEM), Fourier transform infrared spectroscopy  (FTIR), differential thermal analysis (DTA), thermo- gravimetry (TG), photon correlation spectroscopy  (PCS),  zeta  potential  measurements,  and  inductively   coupled  plasma-mass  spectrometry  (ICP-MS).  The   estimated mean crystallite size of GM ZnO NP pow- ders was in the range from 5.8 to 12.1 nm. The syn- thesized  NPs  exhibited  nanosheet  morphology  and   arranged into flake-like aggregates. The toxic poten- tial was investigated in vitro in human hepatocellular  carcinoma cell line HepG2. The thiazolyl blue tetra- zolium bromide (MTS) assay was used to assess cell  viability,  2′,7′-dichlor-fluorescein-diacetate  (DCFH- DA)  assay  to  examine  the  formation  of  intracellu- lar  reactive  oxygen  species  (ROS),  and  comet  assay   to  evaluate  the  genotoxic  response.  GM  ZnO  NPs   slightly reduced HepG2 cell viability, did not induce  ROS formation, and showed low genotoxic potential  at  very  high  doses  (100  μg    mL−1).  ZnO  NPs  fabri- cated  and  modified  using  the  proposed  methodol- ogy deserve further study as potential candidates for  antibacterial agents or dietary supplements with low  overall toxicity.",
publisher = "Springer Science and Business Media B.V.",
journal = "Journal of Nanoparticle Research",
title = "Safe-by-design gelatin-modified zinc oxide nanoparticles",
volume = "23",
number = "9",
doi = "10.1007/s11051-021-05312-3"
}

Janićijević, Ž., Stanković, A., Žegura, B., Veljović, Đ., Đekić, L., Krajišnik, D., Filipič, M.,& Stevanović, M.. (2021). Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research
Springer Science and Business Media B.V.., 23(9).
https://doi.org/10.1007/s11051-021-05312-3

Janićijević Ž, Stanković A, Žegura B, Veljović Đ, Đekić L, Krajišnik D, Filipič M, Stevanović M. Safe-by-design gelatin-modified zinc oxide nanoparticles. in Journal of Nanoparticle Research. 2021;23(9).
doi:10.1007/s11051-021-05312-3 .

Janićijević, Željko, Stanković, Ana, Žegura, Bojana, Veljović, Đorđe, Đekić, Ljiljana, Krajišnik, Danina, Filipič, Metka, Stevanović, Magdalena, "Safe-by-design gelatin-modified zinc oxide nanoparticles" in Journal of Nanoparticle Research, 23, no. 9 (2021),
https://doi.org/10.1007/s11051-021-05312-3 . .

TY  - JOUR
AU  - Bonassi, Stefano
AU  - Ceppi, Marcello
AU  - Møller, Peter
AU  - Azqueta, Amaya
AU  - Milić, Mirta
AU  - Monica, Neri
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine A. S.
AU  - Teixeira, João Paulo
AU  - Bruzzone, , Marco
AU  - Da Silva, Juliana
AU  - Benedetti, Danieli
AU  - Cavallo, Delia
AU  - Ursini, Cinzia Lucia
AU  - Giovannelli, Lisa
AU  - Moretti, Silvia
AU  - Riso, Patrizia
AU  - Del Bo’, Cristian
AU  - Russo, Patrizia
AU  - Dobrzyńska, , Malgorzata
AU  - Goroshinskaya, Irina A.
AU  - Surikova, Ekaterina I.
AU  - Staruchova, Marta
AU  - Barančokova, Magdalena
AU  - Volkovova, Katarina
AU  - Kažimirova, Alena
AU  - Smolkova, Bozena
AU  - Laffon, Blanca
AU  - Valdiglesias, Vanessa
AU  - Pastor, Susana
AU  - Marcos, Ricard
AU  - Hernández, Alba
AU  - Gajski, Goran
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Boutet-Robinet, Elisa
AU  - Perdry, Hervé
AU  - Lebailly, Pierre
AU  - Perez, Carlos L.
AU  - Basaran, Nursen
AU  - Nemeth, , Zsuzsanna
AU  - Safar, Anna
AU  - Dusinska, Maria
AU  - Collins, Andrew
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Pereira, Cristiana Costa
AU  - Costa, Solange
AU  - Gutzkow, Kristine B.
AU  - Ladeira, Carina
AU  - Moretti, Massimo
AU  - Costa, Carla
AU  - Orlow, Irene
AU  - Rojas, Emilio
AU  - Pourrut, Bertrand
AU  - Kruszewski, Marcin
AU  - Knasmueller, Siegfried
AU  - Shaposhnikov, Sergey
AU  - Žegura, Bojana
AU  - Stopper, Helga
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3944
AB  - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
PB  - Nature Research
T2  - Scientific Reports
T1  - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
VL  - 11
IS  - 1
DO  - 10.1038/s41598-021-95976-7
ER  - 

@article{
author = "Bonassi, Stefano and Ceppi, Marcello and Møller, Peter and Azqueta, Amaya and Milić, Mirta and Monica, Neri and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine A. S. and Teixeira, João Paulo and Bruzzone, , Marco and Da Silva, Juliana and Benedetti, Danieli and Cavallo, Delia and Ursini, Cinzia Lucia and Giovannelli, Lisa and Moretti, Silvia and Riso, Patrizia and Del Bo’, Cristian and Russo, Patrizia and Dobrzyńska, , Malgorzata and Goroshinskaya, Irina A. and Surikova, Ekaterina I. and Staruchova, Marta and Barančokova, Magdalena and Volkovova, Katarina and Kažimirova, Alena and Smolkova, Bozena and Laffon, Blanca and Valdiglesias, Vanessa and Pastor, Susana and Marcos, Ricard and Hernández, Alba and Gajski, Goran and Spremo-Potparević, Biljana and Živković, Lada and Boutet-Robinet, Elisa and Perdry, Hervé and Lebailly, Pierre and Perez, Carlos L. and Basaran, Nursen and Nemeth, , Zsuzsanna and Safar, Anna and Dusinska, Maria and Collins, Andrew and Anderson, Diana and Andrade, Vanessa and Pereira, Cristiana Costa and Costa, Solange and Gutzkow, Kristine B. and Ladeira, Carina and Moretti, Massimo and Costa, Carla and Orlow, Irene and Rojas, Emilio and Pourrut, Bertrand and Kruszewski, Marcin and Knasmueller, Siegfried and Shaposhnikov, Sergey and Žegura, Bojana and Stopper, Helga",
year = "2021",
abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death",
volume = "11",
number = "1",
doi = "10.1038/s41598-021-95976-7"
}

Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milić, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, ,. M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., Del Bo’, C., Russo, P., Dobrzyńska, ,. M., Goroshinskaya, I. A., Surikova, E. I., Staruchova, M., Barančokova, M., Volkovova, K., Kažimirova, A., Smolkova, B., Laffon, B., Valdiglesias, V., Pastor, S., Marcos, R., Hernández, A., Gajski, G., Spremo-Potparević, B., Živković, L., Boutet-Robinet, E., Perdry, H., Lebailly, P., Perez, C. L., Basaran, N., Nemeth, ,. Z., Safar, A., Dusinska, M., Collins, A., Anderson, D., Andrade, V., Pereira, C. C., Costa, S., Gutzkow, K. B., Ladeira, C., Moretti, M., Costa, C., Orlow, I., Rojas, E., Pourrut, B., Kruszewski, M., Knasmueller, S., Shaposhnikov, S., Žegura, B.,& Stopper, H.. (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports
Nature Research., 11(1).
https://doi.org/10.1038/s41598-021-95976-7

Bonassi S, Ceppi M, Møller P, Azqueta A, Milić M, Monica N, Brunborg G, Godschalk R, Koppen G, Langie SAS, Teixeira JP, Bruzzone ,M, Da Silva J, Benedetti D, Cavallo D, Ursini CL, Giovannelli L, Moretti S, Riso P, Del Bo’ C, Russo P, Dobrzyńska ,M, Goroshinskaya IA, Surikova EI, Staruchova M, Barančokova M, Volkovova K, Kažimirova A, Smolkova B, Laffon B, Valdiglesias V, Pastor S, Marcos R, Hernández A, Gajski G, Spremo-Potparević B, Živković L, Boutet-Robinet E, Perdry H, Lebailly P, Perez CL, Basaran N, Nemeth ,Z, Safar A, Dusinska M, Collins A, Anderson D, Andrade V, Pereira CC, Costa S, Gutzkow KB, Ladeira C, Moretti M, Costa C, Orlow I, Rojas E, Pourrut B, Kruszewski M, Knasmueller S, Shaposhnikov S, Žegura B, Stopper H. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-95976-7 .

Bonassi, Stefano, Ceppi, Marcello, Møller, Peter, Azqueta, Amaya, Milić, Mirta, Monica, Neri, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, , Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska, , Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević, Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran, Nursen, Nemeth, , Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins, Andrew, Anderson, Diana, Andrade, Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, Stopper, Helga, "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-95976-7 . .