@conference{
author = "Đoković, Jelena and Savić, Sanela and Cekić, Nebojša and Savić, Snežana",
year = "2022",
abstract = "1. INTRODUCTION
Syringeability and injectability are recognised as fundamental performance parameters / critical quality attributes of any parenteral dosage form. Syringeability refers to the ability of an injectable preparation to transfer from a vial through a hypodermic needle prior an injection, while injectability is defined as the force, or pressure, required to inject the formulation from a syringe-needle system into the tissue [1]. When developing drug delivery systems, the priority is usually the release kinetics, biocompatibility or other factors that may come in conflict with the optimal parameters for the applicability of those systems [2]. The aim of this research was to develop a method that could be used for injectability assessment of the intravenous formulations and the application of this method on curcumin-loaded PEGylated nanoemulsions (NEs) in order to gage the impact of PEGylation on NEs injectability.
2. MATERIALS AND METHODS
2.1. Nanoemulsion preparation
Nanoemulsions were prepared using high pressure homogenization method. The aqueous phase (glycerol, polysorbate 80, sodium oleate and highly purified water) was added into the oil phase (soybean oil, soybean lecithin, medium chain triglycerides, butylhydroxytoluene, benzyl alcohol, curcumin and PEGylated phospholipid – PEG2000-DSPE in 0.1 %, 0.3 % or 0.6 % concentrations) and mixed using rotor-stator homogenizer (IKA Ultra-Turrax® T25 digital, IKA®-Werke GmbH and Co. KG, Staufen, Germany), and further processed on high pressure homogenizer (EmulsiFlex-C3, Avestin Inc., Canada) at 800 bar for 10 discontinued cycles. The non PEGylated formulation was marked as CS, and the PEGylated ones were marked S1, S3 and S6, referring to the PEG2000-DSPE concentration.
2.2. Physicochemical characterization
The NEs droplet size (Z-Ave) and droplet size distribution (PDI) were determined with Zetasizer Nano ZS90 (Malvern Instruments Ltd., Worcestershire). Rheological analysis was performed using MCR 302 air-bearing rheometer (Anton Paar, Graz, Austria) equipped with coaxial cylinders system (CC27 measuring bob with C-PTD 180/Air) with sheer rate range of 0.1-100 s-1 at 20°C.
2.3. Injectabilty assesment
The injectability of the NEs was expressed as force (N) needed to extrude the NE in the function of the extruded volume (ml). About 10 ml of the NE was loaded into the 10 ml syringe and extruded through the 25 G scalp vein infusion set (Romed, Wilnis, Netherlands) into the blood mimicking solution, circulating through pump at 4 ml/min, in order to assess the NEs’ performance in the prospective intravenous administration. The NEs were extruded at 1 mm/s croshead speed of the loading cell of the texure analyzer (EZ-LX Compact Table-Top Testing Machine, Shimadzu, Japan) with the TrapeziumX software version 1.5 used for data collection and analysis
3. RESULTS AND DISCUSSION
3.1. Physicochemical characterization
The NEs have average size of about 100 nm, with the PDI values below 0.20, indicating suitability for intravenous application. It could be observed from Fig. 1 that the addition of PEGylated phospholipids caused an increase in NE viscosity, as could be expected given that the polyethylene glycols are used in parenteral suspensions as stabilizing - rheology modifying agents [3].
3.2. Injectability assessment
The injectability assessment was performed with syringe-needle system used in our laboratory for intravenous administration in in vivo animal studies. As blood-mimicking solution, 36.6 %, v/v, glycerol solution was used [4]. It could be observed from Fig. 2 that the injectability of NEs depended on their viscosity, with the higher pressure needed to extrude the formulations with the higher PEG2000-DSPE concentration. Even though, to the best of our knowledge, there are no studies investigating the injectability of the intravenous preparations, based on some previous research on subcutaneous model [5], it is recommended the maximum force used to inject the formulations should be kept about 20 N, which would eliminate S3 and S6 from further investigation (Fig. 2).
4. CONCLUSION
The injectability method used in this research proved as a useful tool in screening formulations adequate for prospective intravenous use.
5. REFERENCES
1. Cilurzo, F., et al. Injectability Evaluation: An Open Issue. AAPS PharmSciTech, 2011. 12(2): 604-609.
2. Sarmadi, M., et al. Modeling, design, and machine learning-based framework for optimal injectability of microparticle-based drug formulations. Science advances, 2020. 6: eabb6594.
3. Gullapalli, R. P., Mazzitelli, C. L. Polyethylene glycols in oral and parenteral formulations—A critical review. International Journal of Pharmaceutics, 2015. 496(2): 219-239.
4. Yousif, M. Y., et al.. Deriving a blood-mimicking fluid for particle image velocimetry in Sylgard-184 vascular models. In Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2009 (pp. 1412-1415
5. Watt, R. P., et al. (2019). Injectability as a function of viscosity and dosing materials for subcutaneous administration. International Journal of Pharmaceutics, 2019:554, 376-386.
ACKNOWLEDGMENT
This research was funded by the MESDT, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161 and supported by the Science Fund of the Republic of Serbia, GRANT No 7749108, Neuroimmune aspects of mood, anxiety and cognitive effects of leads/drug candidates acting at GABAA and/or sigma-2 receptors: In vitro/in vivo delineation by nano- and hiPSC-based platform - NanoCellEmоCog.",
journal = "9th BBBB International conference on pharmaceutical sciences; 15th - 17th September, Ljubljana, Slovenia",
title = "A proposal of innovative injectability assessment method for intravenous formulations - case study on PEGylated nanoemulsions",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4269"
}
