TY  - JOUR
AU  - Rajković, Katarina
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5035
AB  - Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n-6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.
AB  - Juglans nigra (црниорах) каоизворбиолошкиактивнихједињењасекористиутра-диционалнојмедицини. Истраживањебиоактивнихједињењаприсутнихуцрноморахуможедовестидоширеприменењеговихпроизвода. ЗатоовастудијаимазациљдаокарактеришеорашастплодизеленуљускуЈ. nigra наосновухемијскеанализењиховихпетролетарскихиетанолнихекстраката, добијенихултразвучнимирефлуксметодамаекстракције. Антимикробнаактивностразличитихфракцијаекстрактајетестиранако-ришћењемГрам-негативнихбактерија (Escherichia  coli, Pseudomonas  aeruginosa), Грам- -позитивнихбактерија (Enterococcus  faecalis, Staphylococcus  aureus) иквасница (рефе-рентнисојиклиничкиизолатиCandida  albicans). Анализатечномхроматографијомвисокихперформанси, издвојилајеелагинскукиселинукаонајдоминантнијеједињењеуетанолнимекстрактимаорашастогплода ((55,0±1,3)×10-3   kg   m-3) изеленељуске((114,1±0,5)×10-3 g m-3). Неполарнаједињењасуодређенаприменомгаснехроматогра-фскеанализепетролетарскихекстракта. Орашастплодизеленаљускасадржедвезаси-ћенемаснекиселине, палмитинску (C16:0) истеаринскукиселину (C18:0), затиммоно-незасићенемаснекиселине, палмитолеинску  (C16:1n-7),  олеинску  (C18:1n-9)  ивак-ценскукиселину (C18:1n-7), каоиполинезасићенемаснекиселине, линолну (C18:2n-6), γ-линоленску (C18:3n-6) иα-линоленску (C18: 3n-3) киселину. Етанолниекстрактиора-шастогплодаизеленељускепоказалисуантимикробнуактивностнa сојевимаC.  albi-cansкојајенајчешћиузрочникгљивичнихинфекција.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
T1  - Хемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra L.
VL  - 88
IS  - 6
SP  - 603
EP  - 614
DO  - 10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirjana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n-6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections., Juglans nigra (црниорах) каоизворбиолошкиактивнихједињењасекористиутра-диционалнојмедицини. Истраживањебиоактивнихједињењаприсутнихуцрноморахуможедовестидоширеприменењеговихпроизвода. ЗатоовастудијаимазациљдаокарактеришеорашастплодизеленуљускуЈ. nigra наосновухемијскеанализењиховихпетролетарскихиетанолнихекстраката, добијенихултразвучнимирефлуксметодамаекстракције. Антимикробнаактивностразличитихфракцијаекстрактајетестиранако-ришћењемГрам-негативнихбактерија (Escherichia  coli, Pseudomonas  aeruginosa), Грам- -позитивнихбактерија (Enterococcus  faecalis, Staphylococcus  aureus) иквасница (рефе-рентнисојиклиничкиизолатиCandida  albicans). Анализатечномхроматографијомвисокихперформанси, издвојилајеелагинскукиселинукаонајдоминантнијеједињењеуетанолнимекстрактимаорашастогплода ((55,0±1,3)×10-3   kg   m-3) изеленељуске((114,1±0,5)×10-3 g m-3). Неполарнаједињењасуодређенаприменомгаснехроматогра-фскеанализепетролетарскихекстракта. Орашастплодизеленаљускасадржедвезаси-ћенемаснекиселине, палмитинску (C16:0) истеаринскукиселину (C18:0), затиммоно-незасићенемаснекиселине, палмитолеинску  (C16:1n-7),  олеинску  (C18:1n-9)  ивак-ценскукиселину (C18:1n-7), каоиполинезасићенемаснекиселине, линолну (C18:2n-6), γ-линоленску (C18:3n-6) иα-линоленску (C18: 3n-3) киселину. Етанолниекстрактиора-шастогплодаизеленељускепоказалисуантимикробнуактивностнa сојевимаC.  albi-cansкојајенајчешћиузрочникгљивичнихинфекција.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk, Хемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra L.",
volume = "88",
number = "6",
pages = "603-614",
doi = "10.2298/JSC230210024R"
}

Rajković, K., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(6), 603-614.
https://doi.org/10.2298/JSC230210024R

Rajković K, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;88(6):603-614.
doi:10.2298/JSC230210024R .

Rajković, Katarina, Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirjana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society, 88, no. 6 (2023):603-614,
https://doi.org/10.2298/JSC230210024R . .

TY  - JOUR
AU  - Rajković, Katarina M
AU  - Vasić, Marijana
AU  - Drobac, Milica
AU  - Mutić, Jelena
AU  - Jeremić, Sanja
AU  - Simić, Valentina
AU  - Stanković, Jovan
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3604
AB  - The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concen-trations (0–96% (v/v)) and solvent-to-solid ratios (5–20 kg kg−1). The response surfacemethodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) weredeveloped to optimize the extraction variables. The RSM and ANN-GA models determined50% (v/v) ethanol concentration and 20 kg kg−1solvent-to-solid ratio as optimal conditions,ensuring an extraction yield of 27.69 and 27.19 g 100 g−1of dry leaves. The phenolic com-pounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g−1of dry leaves),quercetin-3-O-galactoside (10.99 mg g−1of dry leaves) and quercetin-3-O-rhamnoside (15.07mg g−1of dry leaves) using high-performance liquid chromatography (HPLC). The mineralsin optimal extract were quantified: macro-elements (the relative order by content was: K> Mg > Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) andmicro-elements (the relative order by content was: Zn > Rb > Mn > I>Sr > Ni > Cu > Co >V > Ag > Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extrac-tion coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%).Optimization of extraction process resulted in high extraction yield from J. nigra leaves andoptimal extract containing different phytochemical compounds.
PB  - Elsevier
T2  - Chemical Engineering Research and Design
T1  - Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves
VL  - 157
SP  - 25
EP  - 33
DO  - doi.org/10.1016/j.cherd.2020.03.002
ER  - 

@article{
author = "Rajković, Katarina M and Vasić, Marijana and Drobac, Milica and Mutić, Jelena and Jeremić, Sanja and Simić, Valentina and Stanković, Jovan",
year = "2020",
abstract = "The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concen-trations (0–96% (v/v)) and solvent-to-solid ratios (5–20 kg kg−1). The response surfacemethodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) weredeveloped to optimize the extraction variables. The RSM and ANN-GA models determined50% (v/v) ethanol concentration and 20 kg kg−1solvent-to-solid ratio as optimal conditions,ensuring an extraction yield of 27.69 and 27.19 g 100 g−1of dry leaves. The phenolic com-pounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g−1of dry leaves),quercetin-3-O-galactoside (10.99 mg g−1of dry leaves) and quercetin-3-O-rhamnoside (15.07mg g−1of dry leaves) using high-performance liquid chromatography (HPLC). The mineralsin optimal extract were quantified: macro-elements (the relative order by content was: K> Mg > Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) andmicro-elements (the relative order by content was: Zn > Rb > Mn > I>Sr > Ni > Cu > Co >V > Ag > Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extrac-tion coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%).Optimization of extraction process resulted in high extraction yield from J. nigra leaves andoptimal extract containing different phytochemical compounds.",
publisher = "Elsevier",
journal = "Chemical Engineering Research and Design",
title = "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves",
volume = "157",
pages = "25-33",
doi = "doi.org/10.1016/j.cherd.2020.03.002"
}

Rajković, K. M., Vasić, M., Drobac, M., Mutić, J., Jeremić, S., Simić, V.,& Stanković, J.. (2020). Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research and Design
Elsevier., 157, 25-33.
https://doi.org/doi.org/10.1016/j.cherd.2020.03.002

Rajković KM, Vasić M, Drobac M, Mutić J, Jeremić S, Simić V, Stanković J. Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research and Design. 2020;157:25-33.
doi:doi.org/10.1016/j.cherd.2020.03.002 .

Rajković, Katarina M, Vasić, Marijana, Drobac, Milica, Mutić, Jelena, Jeremić, Sanja, Simić, Valentina, Stanković, Jovan, "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves" in Chemical Engineering Research and Design, 157 (2020):25-33,
https://doi.org/doi.org/10.1016/j.cherd.2020.03.002 . .