TY  - JOUR
AU  - Ilić, Milan
AU  - Samardžić, Stevan
AU  - Kotur-Stevuljević, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3995
AB  - Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents
VL  - 25
IS  - 20
SP  - 6283
EP  - 6294
DO  - 10.26355/eurrev_202110_26998
ER  - 

@article{
author = "Ilić, Milan and Samardžić, Stevan and Kotur-Stevuljević, Jelena and Ušjak, Dušan and Milenković, Marina and Kovačević, Nada and Drobac, Milica",
year = "2021",
abstract = "Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents",
volume = "25",
number = "20",
pages = "6283-6294",
doi = "10.26355/eurrev_202110_26998"
}

Ilić, M., Samardžić, S., Kotur-Stevuljević, J., Ušjak, D., Milenković, M., Kovačević, N.,& Drobac, M.. (2021). Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 25(20), 6283-6294.
https://doi.org/10.26355/eurrev_202110_26998

Ilić M, Samardžić S, Kotur-Stevuljević J, Ušjak D, Milenković M, Kovačević N, Drobac M. Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences. 2021;25(20):6283-6294.
doi:10.26355/eurrev_202110_26998 .

Ilić, Milan, Samardžić, Stevan, Kotur-Stevuljević, Jelena, Ušjak, Dušan, Milenković, Marina, Kovačević, Nada, Drobac, Milica, "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents" in European Review for Medical and Pharmacological Sciences, 25, no. 20 (2021):6283-6294,
https://doi.org/10.26355/eurrev_202110_26998 . .

TY  - CONF
AU  - Antić, Natalija
AU  - Ilić, Milan
AU  - Marčetić, Mirjana
AU  - Drobac, Milica
AU  - Zloh, Mire
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5159
AB  - The Geronrum L. species have significant use in traditionaI medicine. Previous studies showed that these plants are rich in phytochemicals, especially in polyphenols and votatile compounds. The aim of this study was to determine potential target proteins for volatile compounds present in Geranium species and to provide a rationale for its current uses. The structures of identified volatile molecules were collated and their potential protein targets were predicted using PIDGIN software. The 2D and 3D structures of these compounds were generated using KingDraw software, while the 3D structures of selected target proteins
were obtained from Protein Data Bank. All volatile compounds were docked against the whole surface of endothelial PAS domain containing protein 1 (EPAS-1; PDBID: F310) and musarinic receptors M1 (PDBID:6WJC), M2 (PDBID:3UON), M4 (PDBID:5DG) and M5 (PDBID: 6OL9) using LeDock software. The study revealed several compounds with the potential to interact with target
proteins. Hexahydrofarnesyl acetone and γ-curcumene formed the most favorable binding poses inside the binding sites of all muscarinic receptors. Linalool and δ-guaiene forned favorable interactions to EPAS-l target protein. Predicted interaction energies are generally lower compared to ligands for these protein targts, mainly due to their size, hydrophobic properties, and the lack of H-bond forming groups. However, these compounds can fit the target site and form favorable interactions. It can be hypothesized that these plants and their extracts exert pharmacological activity via synergistic action of several components that
can interact with multiple proteins in vivo. Additionally, new therapeutic applications of these plants may be proposed based on the other targets predicted by PIDGIN but not reported in this abstract. Further in vitro studies are required to confirm in silico prediction and gain further insights on potential interactions of tested compounds and target proteins.
PB  - Sciforum
PB  - MDPI
C3  - 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online
T1  - In silico prediction of protein targets for volatile compounds of Geranium L. species
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5159
ER  - 

@conference{
author = "Antić, Natalija and Ilić, Milan and Marčetić, Mirjana and Drobac, Milica and Zloh, Mire",
year = "2021",
abstract = "The Geronrum L. species have significant use in traditionaI medicine. Previous studies showed that these plants are rich in phytochemicals, especially in polyphenols and votatile compounds. The aim of this study was to determine potential target proteins for volatile compounds present in Geranium species and to provide a rationale for its current uses. The structures of identified volatile molecules were collated and their potential protein targets were predicted using PIDGIN software. The 2D and 3D structures of these compounds were generated using KingDraw software, while the 3D structures of selected target proteins
were obtained from Protein Data Bank. All volatile compounds were docked against the whole surface of endothelial PAS domain containing protein 1 (EPAS-1; PDBID: F310) and musarinic receptors M1 (PDBID:6WJC), M2 (PDBID:3UON), M4 (PDBID:5DG) and M5 (PDBID: 6OL9) using LeDock software. The study revealed several compounds with the potential to interact with target
proteins. Hexahydrofarnesyl acetone and γ-curcumene formed the most favorable binding poses inside the binding sites of all muscarinic receptors. Linalool and δ-guaiene forned favorable interactions to EPAS-l target protein. Predicted interaction energies are generally lower compared to ligands for these protein targts, mainly due to their size, hydrophobic properties, and the lack of H-bond forming groups. However, these compounds can fit the target site and form favorable interactions. It can be hypothesized that these plants and their extracts exert pharmacological activity via synergistic action of several components that
can interact with multiple proteins in vivo. Additionally, new therapeutic applications of these plants may be proposed based on the other targets predicted by PIDGIN but not reported in this abstract. Further in vitro studies are required to confirm in silico prediction and gain further insights on potential interactions of tested compounds and target proteins.",
publisher = "Sciforum, MDPI",
journal = "25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online",
title = "In silico prediction of protein targets for volatile compounds of Geranium L. species",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5159"
}

Antić, N., Ilić, M., Marčetić, M., Drobac, M.,& Zloh, M.. (2021). In silico prediction of protein targets for volatile compounds of Geranium L. species. in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online
Sciforum..
https://hdl.handle.net/21.15107/rcub_farfar_5159

Antić N, Ilić M, Marčetić M, Drobac M, Zloh M. In silico prediction of protein targets for volatile compounds of Geranium L. species. in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5159 .

Antić, Natalija, Ilić, Milan, Marčetić, Mirjana, Drobac, Milica, Zloh, Mire, "In silico prediction of protein targets for volatile compounds of Geranium L. species" in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5159 .

TY  - JOUR
AU  - Ilić, Milan D.
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan K.
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3533
AB  - Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.
PB  - Wiley-VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)
VL  - 17
IS  - 2
DO  - 10.1002/cbdv.201900544
ER  - 

@article{
author = "Ilić, Milan D. and Marčetić, Mirjana and Zlatković, Bojan K. and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2020",
abstract = "Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.",
publisher = "Wiley-VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)",
volume = "17",
number = "2",
doi = "10.1002/cbdv.201900544"
}

Ilić, M. D., Marčetić, M., Zlatković, B. K., Lakušić, B., Kovačević, N.,& Drobac, M.. (2020). Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity
Wiley-VHCA AG., 17(2).
https://doi.org/10.1002/cbdv.201900544

Ilić MD, Marčetić M, Zlatković BK, Lakušić B, Kovačević N, Drobac M. Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity. 2020;17(2).
doi:10.1002/cbdv.201900544 .

Ilić, Milan D., Marčetić, Mirjana, Zlatković, Bojan K., Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)" in Chemistry and Biodiversity, 17, no. 2 (2020),
https://doi.org/10.1002/cbdv.201900544 . .

TY  - CONF
AU  - Ilić, Milan
AU  - Drobac, Milica
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Zlatković, Bojan
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5290
AB  - The antimicrobial activity of methanol extracts of aerial parts of 8 Geranium
L. species (G. macrorrhizum L., G. phaeum L., G. sanguineum L., G. robertianum L.,
G. palustre L., G. pyrenaicum Burm. f., G. columbinum L. and G. lucidum L.) was
tested against 8 standard strains (Staphylococcus aureus, Enterococcus faecalis,
Bacillus subtilis, Escherichia coli, Klebsiella pneumaniae, Pseudomonas aeruginosa,
Salmonella abony and Candida albicans) as well as 10 clinical isolates of E. coli.
Also, total phenolics and tannins were quantified using spectrophotometric Folin-
Ciocalteu method. The extracts exhibited antibacterial and anticandidal activity with
minimal inhibitory concentrations (MICs) between 12.5 and 200 μg/ml. E. faecalis
was most sensitive strain (MIC 12.5-50 μg/ml), especially to G. columbinum and G.
phaeum extracts (MICs 12.5 μg/ml). The determined amounts of total phenolics and
tannins were in the range of 170-534 and 38-386 mg GAE/g of dry extract,
respectively, with the highest content in G. sanguineum extract. However, the
antimicrobial activity was not related to total polyphenolics or tannin content and
further investigation in regard with phenolic profile is needed.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Antimicrobial activity of eight Geranium L. species extracts
SP  - 171
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5290
ER  - 

@conference{
author = "Ilić, Milan and Drobac, Milica and Marčetić, Mirjana and Milenković, Marina and Zlatković, Bojan and Kovačević, Nada",
year = "2019",
abstract = "The antimicrobial activity of methanol extracts of aerial parts of 8 Geranium
L. species (G. macrorrhizum L., G. phaeum L., G. sanguineum L., G. robertianum L.,
G. palustre L., G. pyrenaicum Burm. f., G. columbinum L. and G. lucidum L.) was
tested against 8 standard strains (Staphylococcus aureus, Enterococcus faecalis,
Bacillus subtilis, Escherichia coli, Klebsiella pneumaniae, Pseudomonas aeruginosa,
Salmonella abony and Candida albicans) as well as 10 clinical isolates of E. coli.
Also, total phenolics and tannins were quantified using spectrophotometric Folin-
Ciocalteu method. The extracts exhibited antibacterial and anticandidal activity with
minimal inhibitory concentrations (MICs) between 12.5 and 200 μg/ml. E. faecalis
was most sensitive strain (MIC 12.5-50 μg/ml), especially to G. columbinum and G.
phaeum extracts (MICs 12.5 μg/ml). The determined amounts of total phenolics and
tannins were in the range of 170-534 and 38-386 mg GAE/g of dry extract,
respectively, with the highest content in G. sanguineum extract. However, the
antimicrobial activity was not related to total polyphenolics or tannin content and
further investigation in regard with phenolic profile is needed.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Antimicrobial activity of eight Geranium L. species extracts",
pages = "171-171",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5290"
}

Ilić, M., Drobac, M., Marčetić, M., Milenković, M., Zlatković, B.,& Kovačević, N.. (2019). Antimicrobial activity of eight Geranium L. species extracts. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 171-171.
https://hdl.handle.net/21.15107/rcub_farfar_5290

Ilić M, Drobac M, Marčetić M, Milenković M, Zlatković B, Kovačević N. Antimicrobial activity of eight Geranium L. species extracts. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:171-171.
https://hdl.handle.net/21.15107/rcub_farfar_5290 .

Ilić, Milan, Drobac, Milica, Marčetić, Mirjana, Milenković, Marina, Zlatković, Bojan, Kovačević, Nada, "Antimicrobial activity of eight Geranium L. species extracts" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):171-171,
https://hdl.handle.net/21.15107/rcub_farfar_5290 .