TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5016
AB  - Oxidative stress can induce the development of many different disorders. Plant secondary
metabolites may act as antioxidants by neutralisation of free radicals and by stimulation of
endogenous antioxidant mechanisms. One of the important secondary plant metabolites with
antioxidant activity are polyphenols. They can exert activity through different mechanisms
depending on their structure. Polyphenols are widely present in herbal drugs, and some of the
commonly used ones are aronia berry (Aroniae fructus) and bilberry fruit (Myrtilli fructus),
both rich in anthocyanins and tannins and with high antioxidant activity. The main compounds
in turmeric rhizome (Curcumae rhizome) are curcuminoides that manifest antioxidant and anti-
inflammatory activity. Furthermore, tea leaf (Camelliae sinensis folium) and coffee bean
(Coffeae semen), highly present in every-day life, significantly contribute to the daily intake of
antioxidants and provide necessary protection of the organism from the consequences of
oxidative stress.
AB  - Oksidativni stres može indukovati razvoj različitih poremećaja. Biljni sekundarni
metaboliti mogu delovati kao antioksidansi putem neutralizacije slobodnih radikala ili
stimulacijom endogenih antioksidantnih mehanizama. Polifenoli predstavljaju jedne od značajnih
sekundarnih biljnih metabolita sa antioksidantnom aktivnošću. Svoje delovanje mogu ispoljiti
putem različitih mehanizama u zavisnosti od svoje strukture. Polifenoli su široko rasprostranjeni
u biljnim drogama i neke od uobičajeno korišćenih su plod aronije (Aroniae fructus) i borovnice
(Myrtilli fructus), koje su bogate antocijanima i taninima i ispoljavaju visoku antioksidantnu
aktivnost. Glavne komponente u rizomu kurkume (Curcumae rhizome) su kurkuminoidi, koji
pokazuju antioksidantnu i antiinflamatornu aktivnost. Takođe, list čaja (Camelliae sinensis
folium) i seme kafe (Coffeae semen), veoma zastupljeni u svakodnevnom životu, značajno
doprinose dnevnom unosu anioksidanasa i obezbeđuju neophodnu zaštitu organizma od posledica
oksidativnog stresa.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Antioxidant activity of plant secondary metabolites
T1  - Antioksidantna aktivnost biljnih sekundarnih metabolita
VL  - 73
IS  - 4
SP  - 264
EP  - 277
DO  - 10.5937/arhfarm73-45560
ER  - 

@article{
author = "Marčetić, Mirjana and Arsenijević, Jelena",
year = "2023",
abstract = "Oxidative stress can induce the development of many different disorders. Plant secondary
metabolites may act as antioxidants by neutralisation of free radicals and by stimulation of
endogenous antioxidant mechanisms. One of the important secondary plant metabolites with
antioxidant activity are polyphenols. They can exert activity through different mechanisms
depending on their structure. Polyphenols are widely present in herbal drugs, and some of the
commonly used ones are aronia berry (Aroniae fructus) and bilberry fruit (Myrtilli fructus),
both rich in anthocyanins and tannins and with high antioxidant activity. The main compounds
in turmeric rhizome (Curcumae rhizome) are curcuminoides that manifest antioxidant and anti-
inflammatory activity. Furthermore, tea leaf (Camelliae sinensis folium) and coffee bean
(Coffeae semen), highly present in every-day life, significantly contribute to the daily intake of
antioxidants and provide necessary protection of the organism from the consequences of
oxidative stress., Oksidativni stres može indukovati razvoj različitih poremećaja. Biljni sekundarni
metaboliti mogu delovati kao antioksidansi putem neutralizacije slobodnih radikala ili
stimulacijom endogenih antioksidantnih mehanizama. Polifenoli predstavljaju jedne od značajnih
sekundarnih biljnih metabolita sa antioksidantnom aktivnošću. Svoje delovanje mogu ispoljiti
putem različitih mehanizama u zavisnosti od svoje strukture. Polifenoli su široko rasprostranjeni
u biljnim drogama i neke od uobičajeno korišćenih su plod aronije (Aroniae fructus) i borovnice
(Myrtilli fructus), koje su bogate antocijanima i taninima i ispoljavaju visoku antioksidantnu
aktivnost. Glavne komponente u rizomu kurkume (Curcumae rhizome) su kurkuminoidi, koji
pokazuju antioksidantnu i antiinflamatornu aktivnost. Takođe, list čaja (Camelliae sinensis
folium) i seme kafe (Coffeae semen), veoma zastupljeni u svakodnevnom životu, značajno
doprinose dnevnom unosu anioksidanasa i obezbeđuju neophodnu zaštitu organizma od posledica
oksidativnog stresa.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Antioxidant activity of plant secondary metabolites, Antioksidantna aktivnost biljnih sekundarnih metabolita",
volume = "73",
number = "4",
pages = "264-277",
doi = "10.5937/arhfarm73-45560"
}

Marčetić, M.,& Arsenijević, J.. (2023). Antioxidant activity of plant secondary metabolites. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 73(4), 264-277.
https://doi.org/10.5937/arhfarm73-45560

Marčetić M, Arsenijević J. Antioxidant activity of plant secondary metabolites. in Arhiv za farmaciju. 2023;73(4):264-277.
doi:10.5937/arhfarm73-45560 .

Marčetić, Mirjana, Arsenijević, Jelena, "Antioxidant activity of plant secondary metabolites" in Arhiv za farmaciju, 73, no. 4 (2023):264-277,
https://doi.org/10.5937/arhfarm73-45560 . .

TY  - CONF
AU  - Stojanović, Danilo
AU  - Zbiljić, Miloš
AU  - Marčetić, Mirjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5136
AB  - Врсте рода Тhymus су распрострањене у западном медитеранском региону, Азији, јужној Европи и северној Африци. Етарска уља припадника рода Тhymus делују као спазмолитици, експекторанси, антимикробни и антиоксидантни агенси. Циљ рада је био да се одреди биолошко порекло дроге Thymi herba из Грчке на основу макроскопске анализе, састава етарског уља и након упоређивања експерименталних и литературних података. ...
AB  - Thymus species are widespread in the western Mediterranean region, Asia, Southern Europe, and North Africa. Essential oils of Thymus genus members act as spasmolytics, expectorants, antimicrobial, and antioxidant agents. The aim of this study was to determine the biological origin of Thymi herba drug from Greece based on macroscopic analysis, composition of the essential oil, and by comparing experimental and literature data. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад Матица српска
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Одређивање биолошког порекла дроге Thymi herba из Грчке на основу макроскопске анализе и састава етарског уља
T1  - Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5136
ER  - 

@conference{
author = "Stojanović, Danilo and Zbiljić, Miloš and Marčetić, Mirjana",
year = "2023",
abstract = "Врсте рода Тhymus су распрострањене у западном медитеранском региону, Азији, јужној Европи и северној Африци. Етарска уља припадника рода Тhymus делују као спазмолитици, експекторанси, антимикробни и антиоксидантни агенси. Циљ рада је био да се одреди биолошко порекло дроге Thymi herba из Грчке на основу макроскопске анализе, састава етарског уља и након упоређивања експерименталних и литературних података. ..., Thymus species are widespread in the western Mediterranean region, Asia, Southern Europe, and North Africa. Essential oils of Thymus genus members act as spasmolytics, expectorants, antimicrobial, and antioxidant agents. The aim of this study was to determine the biological origin of Thymi herba drug from Greece based on macroscopic analysis, composition of the essential oil, and by comparing experimental and literature data. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад Матица српска, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Одређивање биолошког порекла дроге Thymi herba из Грчке на основу макроскопске анализе и састава етарског уља, Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5136"
}

Stojanović, D., Zbiljić, M.,& Marčetić, M.. (2023). Одређивање биолошког порекла дроге Thymi herba из Грчке на основу макроскопске анализе и састава етарског уља. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад Матица српска..
https://hdl.handle.net/21.15107/rcub_farfar_5136

Stojanović D, Zbiljić M, Marčetić M. Одређивање биолошког порекла дроге Thymi herba из Грчке на основу макроскопске анализе и састава етарског уља. in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5136 .

Stojanović, Danilo, Zbiljić, Miloš, Marčetić, Mirjana, "Одређивање биолошког порекла дроге Thymi herba из Грчке на основу макроскопске анализе и састава етарског уља" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5136 .

TY  - CONF
AU  - Stojanović, Danilo
AU  - Zbiljić, Miloš
AU  - Marčetić, Mirjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5126
AB  - Род Thymus, (Lamiaceae), броји преко 215 врста од којих се многе
примењују у традиционалној медицини и у рационалној
фитотерапији. Користи се херба чији састојци (етарско уље и
флавоноидни хетерозиди) делују као спазмолитици, експекторанси и
као антимикробни и антиоксидантни агенси. Етарска уља припадника
рода се кoристе у хемијској, козметичкој, прехрамбеној и
фармацеутској индустрији. ...
C3  - 2. симпозијум "Трећи век ботанике у Војводини", Нови Сад, 6.октобар
T1  - Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5126
ER  - 

@conference{
author = "Stojanović, Danilo and Zbiljić, Miloš and Marčetić, Mirjana",
year = "2023",
abstract = "Род Thymus, (Lamiaceae), броји преко 215 врста од којих се многе
примењују у традиционалној медицини и у рационалној
фитотерапији. Користи се херба чији састојци (етарско уље и
флавоноидни хетерозиди) делују као спазмолитици, експекторанси и
као антимикробни и антиоксидантни агенси. Етарска уља припадника
рода се кoристе у хемијској, козметичкој, прехрамбеној и
фармацеутској индустрији. ...",
journal = "2. симпозијум "Трећи век ботанике у Војводини", Нови Сад, 6.октобар",
title = "Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5126"
}

Stojanović, D., Zbiljić, M.,& Marčetić, M.. (2023). Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil. in 2. симпозијум "Трећи век ботанике у Војводини", Нови Сад, 6.октобар.
https://hdl.handle.net/21.15107/rcub_farfar_5126

Stojanović D, Zbiljić M, Marčetić M. Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil. in 2. симпозијум "Трећи век ботанике у Војводини", Нови Сад, 6.октобар. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5126 .

Stojanović, Danilo, Zbiljić, Miloš, Marčetić, Mirjana, "Identification of the biological origin of Thymi herba drug from greece based on macroscopic analysis and composition of the essential oil" in 2. симпозијум "Трећи век ботанике у Војводини", Нови Сад, 6.октобар (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5126 .

TY  - CHAP
AU  - Vidović, Bojana
AU  - Marčetić, Mirjana
AU  - Đuriš, Jelena
AU  - Milinčić, Danijel
AU  - Kostić, Aleksandar
AU  - Pešić, Mirjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5270
AB  - Apart from traditional medicine use, the fruit of Lycium barbarum L., known as red goji berry or wolfberry, is recognized and consumed as a functional food. Beyond nutritional properties, goji berry exerts many biological activities and health-promoting effects due to wide-range phytochemicals. Rising global popularity and high demand have spread the production of goji berries from traditional Asian regions into various parts of the world. In addition, other native species, as Lycium ruthenicum Murr., or black goji berry, also have been started to generate attention by the scientific community as a valuable source of nutritional and functional components. This chapter reviews data on nutritional value and bioactive compounds of red and black goji berries from different regions, highlighting the influence of many pre-harvest and post-harvest factors that affect their chemical compositions, sensory quality, and bioactivities.
PB  - Elsevier
T2  - Sustainable Food Science - A Comprehensive Approach
T1  - Goji Berries: Valuable Sources of Nutrients and Bioactive Compounds
VL  - 1-4
SP  - V3-247
EP  - V3-262
DO  - 10.1016/B978-0-12-823960-5.00031-7
ER  - 

@inbook{
author = "Vidović, Bojana and Marčetić, Mirjana and Đuriš, Jelena and Milinčić, Danijel and Kostić, Aleksandar and Pešić, Mirjana",
year = "2023",
abstract = "Apart from traditional medicine use, the fruit of Lycium barbarum L., known as red goji berry or wolfberry, is recognized and consumed as a functional food. Beyond nutritional properties, goji berry exerts many biological activities and health-promoting effects due to wide-range phytochemicals. Rising global popularity and high demand have spread the production of goji berries from traditional Asian regions into various parts of the world. In addition, other native species, as Lycium ruthenicum Murr., or black goji berry, also have been started to generate attention by the scientific community as a valuable source of nutritional and functional components. This chapter reviews data on nutritional value and bioactive compounds of red and black goji berries from different regions, highlighting the influence of many pre-harvest and post-harvest factors that affect their chemical compositions, sensory quality, and bioactivities.",
publisher = "Elsevier",
journal = "Sustainable Food Science - A Comprehensive Approach",
booktitle = "Goji Berries: Valuable Sources of Nutrients and Bioactive Compounds",
volume = "1-4",
pages = "V3-247-V3-262",
doi = "10.1016/B978-0-12-823960-5.00031-7"
}

Vidović, B., Marčetić, M., Đuriš, J., Milinčić, D., Kostić, A.,& Pešić, M.. (2023). Goji Berries: Valuable Sources of Nutrients and Bioactive Compounds. in Sustainable Food Science - A Comprehensive Approach
Elsevier., 1-4, V3-247-V3-262.
https://doi.org/10.1016/B978-0-12-823960-5.00031-7

Vidović B, Marčetić M, Đuriš J, Milinčić D, Kostić A, Pešić M. Goji Berries: Valuable Sources of Nutrients and Bioactive Compounds. in Sustainable Food Science - A Comprehensive Approach. 2023;1-4:V3-247-V3-262.
doi:10.1016/B978-0-12-823960-5.00031-7 .

Vidović, Bojana, Marčetić, Mirjana, Đuriš, Jelena, Milinčić, Danijel, Kostić, Aleksandar, Pešić, Mirjana, "Goji Berries: Valuable Sources of Nutrients and Bioactive Compounds" in Sustainable Food Science - A Comprehensive Approach, 1-4 (2023):V3-247-V3-262,
https://doi.org/10.1016/B978-0-12-823960-5.00031-7 . .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Ilić, Đorđe
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5141
AB  - Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ...
AB  - The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Одељење за природне науке, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)
T1  - Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)
SP  - 14
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5141
ER  - 

@conference{
author = "Marčetić, Mirjana and Ilić, Đorđe and Slavkovska, Violeta and Kovačević, Nada",
year = "2023",
abstract = "Род Satureja L. (Lamiaceae) обухвата преко 30 биљних врста распрострањених у јужној и југоисточној Европи, малој Азији и северној Африци, а најзаступљеније су на источном Медитерану. На Балканском полуострву је присутно 16 врста овог рода које претежно насељавају каменита и сунчана станишта ..., The genus Satureja L. (Lamiaceae) includes over 30 plant species distributed in
southern and southeastern Europe, Asia Minor and North Africa, and they are most abundant
in the eastern Mediterranean. 16 species of this genus are present on the Balkan Peninsula,
which mostly inhabit rocky and sunny habitats. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Одељење за природне науке, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae), Investigation of the essential oil and hydrolates of Satureja subspicata and S. cuneifolia (Lamiaceae)",
pages = "14-15",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5141"
}

Marčetić, M., Ilić, Đ., Slavkovska, V.,& Kovačević, N.. (2023). Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141

Marčetić M, Ilić Đ, Slavkovska V, Kovačević N. Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:14-15.
https://hdl.handle.net/21.15107/rcub_farfar_5141 .

Marčetić, Mirjana, Ilić, Đorđe, Slavkovska, Violeta, Kovačević, Nada, "Испитивање етарског уља и хидролата врста Satureja subspicata и S. cuneifolia (Lamiaceae)" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):14-15,
https://hdl.handle.net/21.15107/rcub_farfar_5141 .

TY  - CONF
AU  - Božić, Dragana
AU  - Ilić, Tijana
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4616
AB  - In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - In vitro assessment of the prebiotic potential of selected plant extracts
SP  - 54
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4616
ER  - 

@conference{
author = "Božić, Dragana and Ilić, Tijana and Marčetić, Mirjana and Samardžić, Stevan and Zdunić, Gordana and Vidović, Bojana",
year = "2022",
abstract = "In recent years, many preclinical studies have provided evidence that polyphenol compounds
and their metabolites exert prebiotic-like effects by selectively stimulating intestinal bacteria
growth and/or activity [1]. In this study, we investigated the chemical composition,
antioxidant capacity, and prebiotic potential of methanol extracts obtained from Prunus
spinosa L. (blackthorn) and Lycium ruthenicum Murray (black goji) fruits. Polyphenolic
profiles were characterized by using HPLC-DAD-ESI-MS and antioxidant capacity was
estimated with DPPH•, ABTS•+, and FRAP assays. The influence of plant extracts (0.312-5
mg/L) on the growth of three probiotic lactobacilli, probiotic yeast (Saccharomyces
boulardii), and two probiotic mixtures was examined. Studied plant extracts were
characterized by different phenolic compound patterns. Hydroxycinnamic acid derivatives,
quercetin glycosides and anthocyanins were detected in blackthorn extract, while black goji
extract was characterized by the presence of hydroxycinnamic acid derivatives and acylated
anthocyanins. The blackthorn fruit extract showed higher antiradical and reduction activity
than those obtained from black goji fruits. Both extracts have influenced the growth of all
tested probiotics in a concentration-dependent manner, especially of yeast, S. boulardii. The
black goji extract had more stimulatory effects, possibly due to higher anthocyanins content.
When the black goji extract was added to the growth medium at the concentration of 5
mg/mL, about 2-fold stimulation of the growth of S. boulardii was observed. In conclusion,
extracts of the black goji and blackthorn fruits promote the growth of probiotics in vitro.
Further studies are still needed to assess the prebiotic potential of these plant extracts.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "In vitro assessment of the prebiotic potential of selected plant extracts",
pages = "54-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4616"
}

Božić, D., Ilić, T., Marčetić, M., Samardžić, S., Zdunić, G.,& Vidović, B.. (2022). In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616

Božić D, Ilić T, Marčetić M, Samardžić S, Zdunić G, Vidović B. In vitro assessment of the prebiotic potential of selected plant extracts. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:54-54.
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

Božić, Dragana, Ilić, Tijana, Marčetić, Mirjana, Samardžić, Stevan, Zdunić, Gordana, Vidović, Bojana, "In vitro assessment of the prebiotic potential of selected plant extracts" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):54-54,
https://hdl.handle.net/21.15107/rcub_farfar_4616 .

TY  - CONF
AU  - Ilić, Tijana
AU  - Samardžić, Stevan
AU  - Zdunić, Gordana
AU  - Božić, Dragana
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4617
AB  - Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.
PB  - University of Belgrade - Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
T1  - Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4617
ER  - 

@conference{
author = "Ilić, Tijana and Samardžić, Stevan and Zdunić, Gordana and Božić, Dragana and Marčetić, Mirjana and Vidović, Bojana",
year = "2022",
abstract = "Lycium ruthenicum Murray (Solanaceae), also known as black goji or black wolfberry, has
been used for centuries in traditional medicine and nutrition in Asian countries [1]. In recent
years, black goji berries have been gaining attention as one of the most valuable sources of
anthocyanins with many health-promoting effects [2]. Therefore, this study aimed to
investigate the phytochemical composition and in vitro biological properties of black goji
berry cultivated in southeastern Serbia. The total phenolics, flavonoids, anthocyanins, and
tannins content were determined spectrophotometrically. HPLC-DAD-ESI-MS analysis was
used to identify the phenolic compounds. Methanol extract from black goji berry was
screened for in vitro antioxidant (DPPH∙, ABTS∙+, FRAP, CUPRAC, and β-carotene bleaching
methods), antimicrobial, and hypoglycemic (α-amylase, α-glucosidase) potential.
Phytochemical screening of the extract confirmed the dominant presence of anthocyanins and
hydroxycinnamic acid derivatives, with petunidin 3-p-cumaroylrutinoside-5 glucoside as the
main phenolic compound [3]. The total phenolic content was 18.13 ± 0.15 mg gallic acid
equivalents/g freeze-dried sample. A high correlation is found between the total phenolic
content and the antioxidant activities using different in vitro antioxidant assays. The
antimicrobial activity of the black goji berry extract against eight laboratory control strains
was not pronounced (MIC > 2 mg/ml). The IC50 values were 6.55 ± 0.38 mg/mL and 7.37 ±
0.02 mg/ml for α-amylase, and α-glucosidase inhibition activity, respectively. Overall,
obtained results suggest that the black goji berry cultivated in Serbia should be considered a
valuable source of bioactive compounds for further use in the food, nutraceutical, and
cosmetic industries.",
publisher = "University of Belgrade - Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts",
title = "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4617"
}

Ilić, T., Samardžić, S., Zdunić, G., Božić, D., Marčetić, M.,& Vidović, B.. (2022). Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts
University of Belgrade - Faculty of Agriculture., 44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617

Ilić T, Samardžić S, Zdunić G, Božić D, Marčetić M, Vidović B. Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray. in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts. 2022;:44-44.
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

Ilić, Tijana, Samardžić, Stevan, Zdunić, Gordana, Božić, Dragana, Marčetić, Mirjana, Vidović, Bojana, "Screening of the phenolic composition and in vitro biological activities of the fruit of Lycium ruthenicum Murray" in 1st European Symposium on Phytochemicals in Medicine and Food (1-EuSPMF) - Book of Abstracts (2022):44-44,
https://hdl.handle.net/21.15107/rcub_farfar_4617 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4610
AB  - The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae
SP  - 167
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4610
ER  - 

@conference{
author = "Marčetić, Mirjana and Vidović, Bojana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana",
year = "2022",
abstract = "The blackthorn is widespread across temperate Europe and also occurs in the Near East and Africa. The fruit is traditionally used in the treatment of digestive, respiratory and kidney disorders. The aim of the study was to investigate composition and the antibacterial activity of methanol extract of blackthorn fruit, and potential prebiotic effect on selected probiotic microorganisms. The blackthorn fruit is a rich source of vitamin C (5.3 mg/100 g, determinated by titration) as well as Ca, Mg and P (106.7; 42.0 and 43.2 mg/100 g, respectively, determinated by ICP-OES). The total phenolic content was 1,235 mg gallic acid/100 g (FC method). Hydroxycinnamic acid derivatives (caffeoylquinic and feruloylquinic acids), flavonoids (glycosides of quercetin, methylquercetin and kaempferol) and anthocyanins (glycosides of cyanidin and peonidin) were tentatively identified by LC-DAD-ESI-MS. The antimicrobial activity on eight laboratory control strains of bacteria was not pronounced (MIC 1.25->5 mg/ml). On the other hand the extract (0.3-5.0 mg/ml) exhibited significant prebiotic effect on investigated probiotic strains of Lactobacillus spp., Saccharomyces boulardii and their mixtures. The stimulation of growth was in the range 4.0-52.8% in concentration dependent manner. In conclusion, significant prebiotic activity of blackthorn fruit confers new insight in blackthorn health benefits.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4610"
}

Marčetić, M., Vidović, B., Samardžić, S., Ilić, T.,& Božić, D.. (2022). Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610

Marčetić M, Vidović B, Samardžić S, Ilić T, Božić D. Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:167-167.
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

Marčetić, Mirjana, Vidović, Bojana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, "Chemical composition and prebiotic effect of the fruit of Prunus spinosa L., Rosaceae" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):167-167,
https://hdl.handle.net/21.15107/rcub_farfar_4610 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4311
AB  - Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.
PB  - MDPI
T2  - Foods
T1  - Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits
VL  - 11
IS  - 20
DO  - 10.3390/foods11203289
ER  - 

@article{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2022",
abstract = "Blackthorn (Prunus spinosa L.) fruit is bluish-black wild fruit traditionally used in nutrition and medicine. It is recently gaining attention as a functional food and an underutilized source of bioactive compounds for application in the food and pharmaceutical industry. This study aimed to assess the health-promoting potential of blackthorn fruits from Serbia by examining their chemical composition and in vitro biological activities. Phytochemical analysis of the blackthorn fruit extracts was performed using LC-DAD-ESI-MS. The total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC) content, antioxidant capacity, and enzyme inhibitory activities were determined spectrophotometrically. The antimicrobial and prebiotic properties were tested using the broth microdilution method. Twenty-seven phenolics belonging to the classes of hydroxybenzoic and hydroxycinnamic acids derivatives, flavonoids, and anthocyanins were identified, with caffeoylquinic acid as the most abundant compound. Blackthorn extracts were characterized by notable TPCs, TFCs, and TACs, and free radical scavenging and reducing ability. The enzyme inhibitory effects (IC50 = 0.43–2.16 mg/mL) were observed towards α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase. Blackthorn fruit extracts in a concentration-dependent manner (0.3–5 mg/mL) stimulated the growth of several probiotic microorganisms and their mixtures, especially the yeast Saccharomyces boulardii. Obtained results support further evaluation of the functional food potential of blackthorn fruit.",
publisher = "MDPI",
journal = "Foods",
title = "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits",
volume = "11",
number = "20",
doi = "10.3390/foods11203289"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2022). Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods
MDPI., 11(20).
https://doi.org/10.3390/foods11203289

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits. in Foods. 2022;11(20).
doi:10.3390/foods11203289 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic Composition, Antioxidant, Anti-Enzymatic, Antimicrobial and Prebiotic Properties of Prunus spinosa L. Fruits" in Foods, 11, no. 20 (2022),
https://doi.org/10.3390/foods11203289 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milinčić, Danijel
AU  - Marčetić, Mirjana
AU  - Đuriš, Jelena
AU  - Ilić, Tijana
AU  - Kostić, Aleksandar
AU  - Pešić, Mirjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4053
AB  - Goji berries have long been used for their nutritional value and medicinal purposes in Asian countries. In the last two decades, goji berries have become popular around the world and are consumed as a functional food due to wide-range bioactive compounds with health-promoting properties. In addition, they are gaining increased research attention as a source of functional ingredients with potential industrial applications. This review focuses on the antioxidant properties of goji berries, scientific evidence on their health effects based on human interventional studies, safety concerns, goji berry processing technologies, and applications of goji berry-based ingredients in developing functional food products.
PB  - MDPI
T2  - Antioxidants
T1  - Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review
VL  - 11
IS  - 2
DO  - 10.3390/antiox11020248
ER  - 

@article{
author = "Vidović, Bojana and Milinčić, Danijel and Marčetić, Mirjana and Đuriš, Jelena and Ilić, Tijana and Kostić, Aleksandar and Pešić, Mirjana",
year = "2022",
abstract = "Goji berries have long been used for their nutritional value and medicinal purposes in Asian countries. In the last two decades, goji berries have become popular around the world and are consumed as a functional food due to wide-range bioactive compounds with health-promoting properties. In addition, they are gaining increased research attention as a source of functional ingredients with potential industrial applications. This review focuses on the antioxidant properties of goji berries, scientific evidence on their health effects based on human interventional studies, safety concerns, goji berry processing technologies, and applications of goji berry-based ingredients in developing functional food products.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review",
volume = "11",
number = "2",
doi = "10.3390/antiox11020248"
}

Vidović, B., Milinčić, D., Marčetić, M., Đuriš, J., Ilić, T., Kostić, A.,& Pešić, M.. (2022). Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review. in Antioxidants
MDPI., 11(2).
https://doi.org/10.3390/antiox11020248

Vidović B, Milinčić D, Marčetić M, Đuriš J, Ilić T, Kostić A, Pešić M. Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review. in Antioxidants. 2022;11(2).
doi:10.3390/antiox11020248 .

Vidović, Bojana, Milinčić, Danijel, Marčetić, Mirjana, Đuriš, Jelena, Ilić, Tijana, Kostić, Aleksandar, Pešić, Mirjana, "Health Benefits and Applications of Goji Berries in Functional Food Products Development: A Review" in Antioxidants, 11, no. 2 (2022),
https://doi.org/10.3390/antiox11020248 . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Stojanović, Danilo
AU  - Marčetić, Mirjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4611
AB  - Teucrium montanum L. is a semi-woody, evergreen small shrub, widely distributed in Europe occurring from the seacoast to the subalpine and alpine belts. The study aimed to investigate and compare the chemical composition of essential oil of aerial parts of T. montanum from 24 populations collected from West, Central, and South West Balkans. The essential oils were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The dominant compounds were oxidized sesquiterpenes (24.83–85.75%), then sesquiterpene hydrocarbons (3.00–62.81%), and oxidized monoterpenes (0–27.78%). Cluster analysis revealed 10 clearly separated groups. First group (Žumberak) is completely separated and characterized by high germacrene-D-4-ol (63.17%), the second (Gjergjevice) by high shyobunol (51.23%), the third (Vratna) by high (dehydro)-sesquicineole-acetate (45.29%), the fourth (Ostrovice) by high epi-α-cadinol (36.80%), the fifth (Nera canyon) by high limonene (36.65%), sixth group (Orovica, Sićevo, Galičica) by high cis-sesquisabinene hydrate (18.73–29.05%), seventh group (Štrpce, Dobrilovina) by high γ-cadinene (13.43–23.06%), eighth group (Tara, Durmitor, Biokovo, Fužine) by moderate germacrene-D-4-ol (19.76–23.44%), and the ninth large group (Potoci, Lovćen, Orjen, Premantura, Vodice, Pletvar, Senj, Oštarije, Trebinje, Korita) by germacren D (6.15–33.87%). Such a high variability needs more research to define relationships among populations.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - The new data on the variability of the essential oil of Teucrium montanum L. from Balkan Peninsula
SP  - 179
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4611
ER  - 

@conference{
author = "Zbiljić, Miloš and Stojanović, Danilo and Marčetić, Mirjana",
year = "2022",
abstract = "Teucrium montanum L. is a semi-woody, evergreen small shrub, widely distributed in Europe occurring from the seacoast to the subalpine and alpine belts. The study aimed to investigate and compare the chemical composition of essential oil of aerial parts of T. montanum from 24 populations collected from West, Central, and South West Balkans. The essential oils were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The dominant compounds were oxidized sesquiterpenes (24.83–85.75%), then sesquiterpene hydrocarbons (3.00–62.81%), and oxidized monoterpenes (0–27.78%). Cluster analysis revealed 10 clearly separated groups. First group (Žumberak) is completely separated and characterized by high germacrene-D-4-ol (63.17%), the second (Gjergjevice) by high shyobunol (51.23%), the third (Vratna) by high (dehydro)-sesquicineole-acetate (45.29%), the fourth (Ostrovice) by high epi-α-cadinol (36.80%), the fifth (Nera canyon) by high limonene (36.65%), sixth group (Orovica, Sićevo, Galičica) by high cis-sesquisabinene hydrate (18.73–29.05%), seventh group (Štrpce, Dobrilovina) by high γ-cadinene (13.43–23.06%), eighth group (Tara, Durmitor, Biokovo, Fužine) by moderate germacrene-D-4-ol (19.76–23.44%), and the ninth large group (Potoci, Lovćen, Orjen, Premantura, Vodice, Pletvar, Senj, Oštarije, Trebinje, Korita) by germacren D (6.15–33.87%). Such a high variability needs more research to define relationships among populations.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "The new data on the variability of the essential oil of Teucrium montanum L. from Balkan Peninsula",
pages = "179-179",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4611"
}

Zbiljić, M., Stojanović, D.,& Marčetić, M.. (2022). The new data on the variability of the essential oil of Teucrium montanum L. from Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 179-179.
https://hdl.handle.net/21.15107/rcub_farfar_4611

Zbiljić M, Stojanović D, Marčetić M. The new data on the variability of the essential oil of Teucrium montanum L. from Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:179-179.
https://hdl.handle.net/21.15107/rcub_farfar_4611 .

Zbiljić, Miloš, Stojanović, Danilo, Marčetić, Mirjana, "The new data on the variability of the essential oil of Teucrium montanum L. from Balkan Peninsula" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):179-179,
https://hdl.handle.net/21.15107/rcub_farfar_4611 .

TY  - JOUR
AU  - Stojanović, Danilo
AU  - Marčetić, Mirjana
AU  - Ušjak, Dušan
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4061
AB  - Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin.
AB  - Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)
T1  - Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)
VL  - 79
IS  - 1
SP  - 62
EP  - 68
DO  - 10.2298/VSP200508083S
ER  - 

@article{
author = "Stojanović, Danilo and Marčetić, Mirjana and Ušjak, Dušan and Milenković, Marina",
year = "2022",
abstract = "Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin., Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae), Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)",
volume = "79",
number = "1",
pages = "62-68",
doi = "10.2298/VSP200508083S"
}

Stojanović, D., Marčetić, M., Ušjak, D.,& Milenković, M.. (2022). Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 79(1), 62-68.
https://doi.org/10.2298/VSP200508083S

Stojanović D, Marčetić M, Ušjak D, Milenković M. Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled. 2022;79(1):62-68.
doi:10.2298/VSP200508083S .

Stojanović, Danilo, Marčetić, Mirjana, Ušjak, Dušan, Milenković, Marina, "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)" in Vojnosanitetski pregled, 79, no. 1 (2022):62-68,
https://doi.org/10.2298/VSP200508083S . .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4319
AB  - This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far.
AB  - Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)
T1  - Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)
VL  - 46
IS  - 2
SP  - 269
EP  - 284
DO  - 10.2298/BOTSERB2202269S
ER  - 

@article{
author = "Slavkovska, Violeta and Zlatković, Bojan and Marčetić, Mirjana and Kovačević, Nada and Drobac, Milica",
year = "2022",
abstract = "This work was aimed at investigating the anatomy and composition of the essential oils from different organs of two Pimpinella species, P. tragium and P. saxifraga, originating from the central part of the Balkan Peninsula. The results of the analysis of the anatomy of the different organs of P. tragium and P. saxifraga showed that they were very similar in terms of the structure of the roots, stems and petioles. The roots showed a secondary structure and the stems had a primary structure with closed collateral vascular bundles. The petioles were cordatum, ribbed with an adaxial groove, and sparsely covered with short unior bicellular non-glandular trichomes with a pointed top (both species) or with long uniseriate, multicellular, non-glandular trichomes (P. saxifraga). Differences were observed in the anatomy of the leaves and in the indumentum of the fruits. The leaves of P. tragium were isobilateral, while those of P. saxifraga were dorsiventral. The P. tragium fruits were pubescent, covered with numerous, multicellular, uniseriate, straight or curved, gradually acuminate non-glandular trichomes, whereas the fruits of P. saxifraga were glabrous. These differences are of ecological and taxonomic significance. Secretory canals were observed in all the investigated organs of both species. The essential oils, isolated by hydrodistillation from the aerial parts with inflorescences, the roots from the flowering and fruiting period, and the fruits of both species, were analysed by GC-FID/MS. Although P. tragium and P. saxifraga essential oils showed certain differences in qualitative and quantitative patterns, all the oils were characterised by the presence of pseudoisoeugenol type phenylpropanoid epoxy-pseudoisoeugenyl-2-methylbutyrate, as well as by that of trinorsesquiterpenes pregeijerene, geijerene and trinoranastreptene. In addition, azulenes were present in all the root essential oils, whereas in the majority of the oils from the fruits and flowering aerial parts the common compound was the sesquiterpene β-bisabolene. The occurrence of phenylpropanoids of pseudoisoeugenol type and geijerenes is important from the chemotaxonomic point of view and confirms them as chemical markers of the Pimpinella species analysed so far., Cilj ovog istraživanja bio je analiza anatomske građe i hemijskog sastava etarskih ulja različitih organa dve vrste roda Pimpinella, P.
tragium i P. saxifraga, poreklom iz centralnog dela Balkanskog poluostrva. Na osnovu rezultata analize unutrašnje građe različitih
organa P. tragium i P. saxifraga može se zaključiti da postoje velike sličnosti u građi korena, stabla i lisne drške. Koren obe vrste bio
je sekundarne građe, stablo primarne građe sa zatvorenim kolateralnim sprovodnim snopićima, a lisne drške srcolike, rebraste sa
adaksijalnim žlebom, sporadično prekrivene kratkim jednoćelijskim ili dvoćelijskim, na vrhu zašiljenim nežlezdanim trihomama
(obe vrste) ili dugim uniserijatnim, višećelijskim nežlezdanim trihomama (P. saxifraga). Uočene su razlike u anatomiji listova i in-
dumentumu plodova ove dve vrste. Listovi P. tragium bili su izobilateralni, a P. saxifraga dorziventralni. Plodovi P. tragium bili su
maljavi, prekriveni brojnim, višećelijskim, uniserijatnim, uspravnim ili zakrivljenim, postepeno zašiljenim nežlezdanim trihomama,
dok su plodovi P. saxifraga bili goli. Ove razlike u anatomskoj građi su od ekološkog i taksonomskog značaja. U svim organima obe
ispitivane vrste uočeno je prisustvo sekretornih kanala. Etarska ulja su izolovana postupkom destilacije vodenom parom iz različitih
organa obe vrste, nadzemnog dela sa cvastima, korena prikupljenog u fazi cvetanja i u fazi plodonošenja i ploda i analizirana GC-FID/
MS metodom. Iako su ispitivana etarska ulja P. tragium i P. saxifraga bila donekle različitog kvalitativnog i kvantitativnog sastava,
za sva etarska ulja bilo je karakteristično prisustvo fenilpropanoida pseudizoeugenol tipa epoksi-pseudoizoeugenil-2-metilbutirata,
kao i trinorseskviterpena pregeijerena, geijerena i trinoranastreptena. Pored toga, u svim etarskim uljima dobijenim iz korena bili su
prisutni azuleni, a u većini etarskih ulja plodova i nadzemnih delova u cvetu karakterističan sastojak bio je sekviterpen β-bisabolen.
Prisustvo fenilpropanoida pseudoizoeugenol tipa i trinorsekviterpena tipa geijerena značajno je sa hemotaksonomskog aspekta i pot-
vrđuje njihovu ulogu hemijskih markera do sada ispitivanih Pimpinella vrsta.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae), Anatomska građa i sastav etarskih ulja različitih organa vrsta Pimpinella
tragium i P. saxifraga (Apiaceae)",
volume = "46",
number = "2",
pages = "269-284",
doi = "10.2298/BOTSERB2202269S"
}

Slavkovska, V., Zlatković, B., Marčetić, M., Kovačević, N.,& Drobac, M.. (2022). The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 46(2), 269-284.
https://doi.org/10.2298/BOTSERB2202269S

Slavkovska V, Zlatković B, Marčetić M, Kovačević N, Drobac M. The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae). in Botanica Serbica. 2022;46(2):269-284.
doi:10.2298/BOTSERB2202269S .

Slavkovska, Violeta, Zlatković, Bojan, Marčetić, Mirjana, Kovačević, Nada, Drobac, Milica, "The anatomy and essential oil composition of the different organs of Pimpinella tragium and P. saxifraga (Apiaceae)" in Botanica Serbica, 46, no. 2 (2022):269-284,
https://doi.org/10.2298/BOTSERB2202269S . .

TY  - CONF
AU  - Marčetić, Mirjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4447
AB  - To define new herbal drug and its quality, it is important to know inter- and intraspecific
variability of chemical constituents of certain species of one genus. In the case of potentially new
aromatic herbal drugs, among others, variability of essential oils composition is examined. The
aim was to investigate interspecific variability of essential oil composition of four Seseli L.
species, Apiaceae and intraspecific essential oil variability of selected species S. rigidum Waldst.
& Kit. Composition of oils was determined by GC-FID and GC-MS and results were statistically
analysed (ANOVA, PCA, CDA, cluster analysis). Antimicrobial (broth-microdilution,
checkerboard method), cytotoxic (MTT test, flow cytometry) and genotoxic/antigenotoxic activity
(Comet test) of oils of selected populations of S. rigidum were investigated. Significant differences
in oils composition of investigated Seseli species were observed. Monoterpene hydrocarbons
dominated in oils of aerial parts of S. rigidum and S. gracile Waldst. & Kit. and sesquiterpene
hydrocarbons in S. annuum L. and S. tomentosum Vis. Climate had significant influence on
composition, higher than population and substrate (serpentine or calcareous). The best
antimicrobial activity showed S. rigidum root oil of population with high falcarinol content against
Candida albicans and MRSA. Synergistic effect of mentioned oil and antibiotics was observed.
Oils of root, aerial parts and fruit showed cytotoxic activity against tumor cells HeLa, LS174 and
A549, while genotoxic effects weren’t observed. Oils of S. rigidum aerial parts and fruit
manifested antigenotoxic effects. Conducted research indicated the potential of S. rigidum as a
source of new herbal medicinal raw materials.
AB  - Za definisanje nove biljne droge i njenog kvaliteta važno je poznavati kako inter-, tako
i intraspecijsku varijabilnost hemijskih sastojaka određenih vrsta jednog roda. U slučaju
potencijalno novih aromatičnih biljnih droga, između ostalog, ispituje se varijabilnost sastava
etarskih ulja. Cilj rada je bilo ispitivanje interspecijske varijabilnosti sastava etarskih ulja
četiri vrste roda Seseli L., Apiaceae, kao i intraspecijske varijabilnosti etarskih ulja odabrane
vrste S. rigidum Waldst. & Kit. Sastav etarskih ulja utvrđen je GC-FID i GC-MS metodom i
rezultati su statistički analizirani (ANOVA, PCA, CDA, klaster analiza). Ispitane su
antimikrobna (bujon-mikrodiluciona, checkerboard metoda), citotoksična (MTT test,
protočna citometrija) i genotoksična/antigenotoksična aktivnost (Komet test) etarskih ulja
odabranih populacija S. rigidum. Uočene su značajne razlike u sastavu etarskih ulja
ispitivanih vrsta roda Seseli. U etarskim uljima herbi S. rigidum i S. gracile Waldst. & Kit.
dominirali su monoterpenski ugljovodonici, a u uljima herbi S. annuum L. i S. tomentosum Vis.
seskviterpenski ugljovodonici. Na sastav etarskih ulja najznačajnije je uticala klima, a znatno
manje ispitivana populacija i podloga (serpentinit ili krečnjak). Najbolju antimikrobnu
aktivnost pokazalo je etarsko ulje korena S. rigidum populacije sa visokim sadržajem
falkarinola prema sojevima Candida albicans i MRSA. Takođe, uočen je sinergistički efekat
navedenog ulja i određenih antibiotika. Etarska ulja korena, herbe i ploda S. rigidum ispoljila
su i citotoksičnu aktivnost prema tumorskim ćelijama HeLa, LS174 i A549, dok genotoksično
delovanje nije uočeno. Dodatno, etarska ulja herbe i ploda S. rigidum ostvarila su
antigenotoksično delovanje. Sprovedeno ispitivanje ukazalo je na potencijal vrste S. rigidum
kao izvora novih biljnih lekovitih sirovina.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Inter- and intraspecific variability as a basis for defining the quality of herbal drug
T1  - Inter‐ i intraspecijska varijabilnost kao osnov za definisanje kvaliteta biljne droge
VL  - 72
IS  - 4 suplement
SP  - S60
EP  - S61
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4447
ER  - 

@conference{
author = "Marčetić, Mirjana",
year = "2022",
abstract = "To define new herbal drug and its quality, it is important to know inter- and intraspecific
variability of chemical constituents of certain species of one genus. In the case of potentially new
aromatic herbal drugs, among others, variability of essential oils composition is examined. The
aim was to investigate interspecific variability of essential oil composition of four Seseli L.
species, Apiaceae and intraspecific essential oil variability of selected species S. rigidum Waldst.
& Kit. Composition of oils was determined by GC-FID and GC-MS and results were statistically
analysed (ANOVA, PCA, CDA, cluster analysis). Antimicrobial (broth-microdilution,
checkerboard method), cytotoxic (MTT test, flow cytometry) and genotoxic/antigenotoxic activity
(Comet test) of oils of selected populations of S. rigidum were investigated. Significant differences
in oils composition of investigated Seseli species were observed. Monoterpene hydrocarbons
dominated in oils of aerial parts of S. rigidum and S. gracile Waldst. & Kit. and sesquiterpene
hydrocarbons in S. annuum L. and S. tomentosum Vis. Climate had significant influence on
composition, higher than population and substrate (serpentine or calcareous). The best
antimicrobial activity showed S. rigidum root oil of population with high falcarinol content against
Candida albicans and MRSA. Synergistic effect of mentioned oil and antibiotics was observed.
Oils of root, aerial parts and fruit showed cytotoxic activity against tumor cells HeLa, LS174 and
A549, while genotoxic effects weren’t observed. Oils of S. rigidum aerial parts and fruit
manifested antigenotoxic effects. Conducted research indicated the potential of S. rigidum as a
source of new herbal medicinal raw materials., Za definisanje nove biljne droge i njenog kvaliteta važno je poznavati kako inter-, tako
i intraspecijsku varijabilnost hemijskih sastojaka određenih vrsta jednog roda. U slučaju
potencijalno novih aromatičnih biljnih droga, između ostalog, ispituje se varijabilnost sastava
etarskih ulja. Cilj rada je bilo ispitivanje interspecijske varijabilnosti sastava etarskih ulja
četiri vrste roda Seseli L., Apiaceae, kao i intraspecijske varijabilnosti etarskih ulja odabrane
vrste S. rigidum Waldst. & Kit. Sastav etarskih ulja utvrđen je GC-FID i GC-MS metodom i
rezultati su statistički analizirani (ANOVA, PCA, CDA, klaster analiza). Ispitane su
antimikrobna (bujon-mikrodiluciona, checkerboard metoda), citotoksična (MTT test,
protočna citometrija) i genotoksična/antigenotoksična aktivnost (Komet test) etarskih ulja
odabranih populacija S. rigidum. Uočene su značajne razlike u sastavu etarskih ulja
ispitivanih vrsta roda Seseli. U etarskim uljima herbi S. rigidum i S. gracile Waldst. & Kit.
dominirali su monoterpenski ugljovodonici, a u uljima herbi S. annuum L. i S. tomentosum Vis.
seskviterpenski ugljovodonici. Na sastav etarskih ulja najznačajnije je uticala klima, a znatno
manje ispitivana populacija i podloga (serpentinit ili krečnjak). Najbolju antimikrobnu
aktivnost pokazalo je etarsko ulje korena S. rigidum populacije sa visokim sadržajem
falkarinola prema sojevima Candida albicans i MRSA. Takođe, uočen je sinergistički efekat
navedenog ulja i određenih antibiotika. Etarska ulja korena, herbe i ploda S. rigidum ispoljila
su i citotoksičnu aktivnost prema tumorskim ćelijama HeLa, LS174 i A549, dok genotoksično
delovanje nije uočeno. Dodatno, etarska ulja herbe i ploda S. rigidum ostvarila su
antigenotoksično delovanje. Sprovedeno ispitivanje ukazalo je na potencijal vrste S. rigidum
kao izvora novih biljnih lekovitih sirovina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Inter- and intraspecific variability as a basis for defining the quality of herbal drug, Inter‐ i intraspecijska varijabilnost kao osnov za definisanje kvaliteta biljne droge",
volume = "72",
number = "4 suplement",
pages = "S60-S61",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4447"
}

Marčetić, M.. (2022). Inter- and intraspecific variability as a basis for defining the quality of herbal drug. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S60-S61.
https://hdl.handle.net/21.15107/rcub_farfar_4447

Marčetić M. Inter- and intraspecific variability as a basis for defining the quality of herbal drug. in Arhiv za farmaciju. 2022;72(4 suplement):S60-S61.
https://hdl.handle.net/21.15107/rcub_farfar_4447 .

Marčetić, Mirjana, "Inter- and intraspecific variability as a basis for defining the quality of herbal drug" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S60-S61,
https://hdl.handle.net/21.15107/rcub_farfar_4447 .

TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3941
AB  - Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.
PB  - Department of Biology, Faculty of Science, University of Zagreb
T2  - Acta Botanica Croatica
T1  - Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia
VL  - 80
IS  - 1
SP  - 48
EP  - 55
DO  - 10.37427/botcro-2020-033
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2021",
abstract = "Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.",
publisher = "Department of Biology, Faculty of Science, University of Zagreb",
journal = "Acta Botanica Croatica",
title = "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia",
volume = "80",
number = "1",
pages = "48-55",
doi = "10.37427/botcro-2020-033"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2021). Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica
Department of Biology, Faculty of Science, University of Zagreb., 80(1), 48-55.
https://doi.org/10.37427/botcro-2020-033

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica. 2021;80(1):48-55.
doi:10.37427/botcro-2020-033 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia" in Acta Botanica Croatica, 80, no. 1 (2021):48-55,
https://doi.org/10.37427/botcro-2020-033 . .

TY  - CONF
AU  - Ilić, Tijana
AU  - Krgović, Nemanja
AU  - Marčetić, Mirjana
AU  - Vidović, Bojana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5096
AB  - Due to the presence of many nutrients and bioactive compounds, fruits of Lycium species (Fructus Lycii, goji berries) are recognized as a “superfood,” which regular consumption might exert many beneficial health effects. Therefore, in addition to China and other Asian countries, the cultivation of Lycium species has become widespread throughout Europe. This study investigated the total phenolics (TPC), total flavonoids (TFC) and polysaccharides content, as well as biological properties of goji berry (Lycium barbarum L.) cultivated in the Belgrade region, Serbia. The HPLC method was used for the identification of individual phenolic compounds (rutin, quercetin-diglucuronide, chlorogenic acid and caffeic acid). Antioxidant properties of goji berry extracts with different solvent were evaluated for radical scavenging (DPPH, ABTS), reducing power (CUPRAC, FRAP), and β-carotene/linoleic acid bleaching inhibition capacity. Additionally, goji berry extracts were screened for their anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase, and anti-acetylcholinesterase activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest contents of TPC (3.76 mg GAE/g dry weight), TFC (0.65 mg CE/g dry weight), as well as superior antioxidant activity evaluated by all assays. Also, the methanol extract of goji berry inhibited all tested enzymes in concentration-dependent manners. The highest enzyme inhibitory activity was shown for tyrosinase (IC50 of 1.4 mg ± 0.01 mg/mL). The IC50 values were 4.66 ± 0.25 mg/mL, 10.68 ± 0.07 mg/mL, 7.07 ± 0.1 mg/mL for α-amylase, α-glucosidase and acetylcholinesterase inhibition activity, respectively. Overall, obtained results suggest that goji berry cultivated in Serbia is a valuable source of bioactive compounds that can be use in the food, nutraceutical, and cosmetic industries.
PB  - University of Belgrade
C3  - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
T1  - Phytochemical composition and in vitro biological activities of goji berry cultivated in Serbia
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5096
ER  - 

@conference{
author = "Ilić, Tijana and Krgović, Nemanja and Marčetić, Mirjana and Vidović, Bojana",
year = "2021",
abstract = "Due to the presence of many nutrients and bioactive compounds, fruits of Lycium species (Fructus Lycii, goji berries) are recognized as a “superfood,” which regular consumption might exert many beneficial health effects. Therefore, in addition to China and other Asian countries, the cultivation of Lycium species has become widespread throughout Europe. This study investigated the total phenolics (TPC), total flavonoids (TFC) and polysaccharides content, as well as biological properties of goji berry (Lycium barbarum L.) cultivated in the Belgrade region, Serbia. The HPLC method was used for the identification of individual phenolic compounds (rutin, quercetin-diglucuronide, chlorogenic acid and caffeic acid). Antioxidant properties of goji berry extracts with different solvent were evaluated for radical scavenging (DPPH, ABTS), reducing power (CUPRAC, FRAP), and β-carotene/linoleic acid bleaching inhibition capacity. Additionally, goji berry extracts were screened for their anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase, and anti-acetylcholinesterase activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest contents of TPC (3.76 mg GAE/g dry weight), TFC (0.65 mg CE/g dry weight), as well as superior antioxidant activity evaluated by all assays. Also, the methanol extract of goji berry inhibited all tested enzymes in concentration-dependent manners. The highest enzyme inhibitory activity was shown for tyrosinase (IC50 of 1.4 mg ± 0.01 mg/mL). The IC50 values were 4.66 ± 0.25 mg/mL, 10.68 ± 0.07 mg/mL, 7.07 ± 0.1 mg/mL for α-amylase, α-glucosidase and acetylcholinesterase inhibition activity, respectively. Overall, obtained results suggest that goji berry cultivated in Serbia is a valuable source of bioactive compounds that can be use in the food, nutraceutical, and cosmetic industries.",
publisher = "University of Belgrade",
journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts",
title = "Phytochemical composition and in vitro biological activities of goji berry cultivated in Serbia",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5096"
}

Ilić, T., Krgović, N., Marčetić, M.,& Vidović, B.. (2021). Phytochemical composition and in vitro biological activities of goji berry cultivated in Serbia. in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
University of Belgrade., 110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5096

Ilić T, Krgović N, Marčetić M, Vidović B. Phytochemical composition and in vitro biological activities of goji berry cultivated in Serbia. in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:110-110.
https://hdl.handle.net/21.15107/rcub_farfar_5096 .

Ilić, Tijana, Krgović, Nemanja, Marčetić, Mirjana, Vidović, Bojana, "Phytochemical composition and in vitro biological activities of goji berry cultivated in Serbia" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):110-110,
https://hdl.handle.net/21.15107/rcub_farfar_5096 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Samardžić, Stevan
AU  - Ilić, Tijana
AU  - Božić, Dragana
AU  - Vidović, Bojana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5097
AB  - The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.
PB  - University of Belgrade
C3  - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
T1  - Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5097
ER  - 

@conference{
author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana",
year = "2021",
abstract = "The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.",
publisher = "University of Belgrade",
journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts",
title = "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5097"
}

Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2021). Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts
University of Belgrade., 85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097

Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:85-85.
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):85-85,
https://hdl.handle.net/21.15107/rcub_farfar_5097 .

TY  - JOUR
AU  - Suručić, Relja
AU  - Kundaković-Vasović, Tatjana
AU  - Marčetić, Mirjana
AU  - Drakul, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4002
AB  - Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil.
AB  - Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil
T1  - Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)
VL  - 78
IS  - 10
SP  - 1065
EP  - 1071
DO  - 10.2298/VSP190320028S
ER  - 

@article{
author = "Suručić, Relja and Kundaković-Vasović, Tatjana and Marčetić, Mirjana and Drakul, Dragana and Milenković, Marina and Kovačević, Nada",
year = "2021",
abstract = "Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil., Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil, Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)",
volume = "78",
number = "10",
pages = "1065-1071",
doi = "10.2298/VSP190320028S"
}

Suručić, R., Kundaković-Vasović, T., Marčetić, M., Drakul, D., Milenković, M.,& Kovačević, N.. (2021). Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 78(10), 1065-1071.
https://doi.org/10.2298/VSP190320028S

Suručić R, Kundaković-Vasović T, Marčetić M, Drakul D, Milenković M, Kovačević N. Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled. 2021;78(10):1065-1071.
doi:10.2298/VSP190320028S .

Suručić, Relja, Kundaković-Vasović, Tatjana, Marčetić, Mirjana, Drakul, Dragana, Milenković, Marina, Kovačević, Nada, "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil" in Vojnosanitetski pregled, 78, no. 10 (2021):1065-1071,
https://doi.org/10.2298/VSP190320028S . .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Marčetić, Mirjana
AU  - Drobac, Milica
AU  - Slavkovska, Violeta
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4669
AB  - Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions.
AB  - Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Polyphenolic composition of commercial samples of rtanj tea
T1  - Polifenolni sastav komercijalnih uzoraka rtanjskog čaja
VL  - 71
IS  - 5 suplement
SP  - S54
EP  - S55
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4669
ER  - 

@conference{
author = "Arsenijević, Jelena and Marčetić, Mirjana and Drobac, Milica and Slavkovska, Violeta and Kovačević, Nada",
year = "2021",
abstract = "Rtanj’s tea infusion is often used to relieve respiratory, urinary and other ailments, as
well as a tonic or a beverage. Commercially available Rtanj tea represents a herb obtained
mainly from wild plants. As a biological source manufacturers list several species of the
genus Satureja L., i.e. Satureja kitaibelii Wierzb. ex Heuff., S. montana L. and S. hortensis L.
This diversity may affect the composition of infusions, their effects and organoleptic features.
In this work, we investigated the polyphenolic profile of infusions (2 g/100 mL)
prepared from seven commercially available samples of Rtanj tea. The LC-MS method was
used to identify polyphenols as well as to determine the content of marker compounds,
rosmarinic acid (RA) and clinopodic acid O (CAO), by using linear regression of rosmarinic
acid as an external standard. The content of total polyphenols was determined by the
spectrophotometric method based on the Folin-Ciocalteu reaction, and the results were
expressed in gallic acid (GA) equivalents/L of infusion.
LC-MS analysis revealed that the qualitative composition of the tested infusions was
very similar. Based on the UV and MS data, phenolic acids were identified in the infusions, of
which RA and/or CAO were the dominant compounds, as well as flavonoids, among which
luteolin heteroside prevailed. On the other hand, infusions differed in quantitative
composition. The content of total polyphenols varied between 118.3 and 476.2 mg/L GA, the
content of RA 19.5–129.5 mg L, while the content of CAO in infusions was 13.9–72.3 mg/L,
calculated as RA. Higher content of total polyphenols and RA was determined in the infusions
of the samples in the form of filter bags than those found in the form of non-comminuted
herb. In addition, it was observed that the origin of plant material had an impact on the
quantity ratio between RA and CAO.
The results obtained in this study indicate the importance of the selection of the
biological source and its correct identification, as well as the primary processing of the herb
on the composition of the infusions., Rtanjski čaj se često upotrebljava u obliku infuza za ublažavanje respiratornih,
urinarnih i drugih tegoba, kao tonik ili napitak [1]. Komercijalno dostupni rtanjski čaj
predstavlja herbu dobijenu uglavnom od samoniklih biljaka, a kao biološki izvor proizvođači
navode različite vrste roda Satureja L. (fam. Lamiaceae), Satureja kitaibelii Wierzb. ex Heuff.,
S. montana L. i S. hortensis L., što može imati uticaj na sastav infuza, njegove efekte i
organoleptičke karakteristike.
U ovom radu ispitan je polifenolni profil infuza (2 g/100 mL) pripremljenih od sedam
komercijalno dostupnih uzoraka rtanjskog čaja. LC-MS metodom izvršena je identifikacija
polifenola kao i određivanje sadržaja marker jedinjenja, rozmarinske kiseline (RK) i
klinopodinske kiseline O (KKO), primenom linearne regresije korišćenjem rozmarinske
kiseline kao eksternog standarda. Spektrofotometrijskom metodom na bazi Folin‐Ciocalteu
reakcije određen je sadržaj ukupnih polifenola, a rezultati su izraženi u ekvivalentima galne
kiseline (GA)/L infuza.
LC-MS analizom ustanovljeno je da je kvalitativni sastav ispitivanih infuza međusobno
vrlo sličan. U infuzima su, na osnovu UV i MS podataka, identifikovane fenolkarboksilne
kiseline, od kojih su RK i/ili KKO dominantna jedinjenja, kao i flavonoidi, među kojima se
ističe prisustvo heterozida luteolina. Sa druge strane, infuzi su se međusobno razlikovali u
kvantitativnom sastavu. Sadržaj ukupnih polifenola varirao je između 118,3 i 476,2 mg GA/L,
sadržaj RK 19,5–129,5 mg/L, dok je sadržaj KKO u infuzima iznosio 13,9–72,3 mg/L,
računato kao RK. Veći sadržaj ukupnih polifenola i RK određen je u infuzima uzoraka u
obliku filter vrećica nego onih koji se nalaze u obliku neusitnjene herbe. Pored toga, može se
uočiti da poreklo biljnog materijala ima uticaj na međusobni odnos RK i KKO.
Rezultati dobijeni u ovom ispitivanju ukazuju na značaj odabira biološkog izvora,
njegove pravilne identifikacije i primarne prerade herbe na sastav infuza.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Polyphenolic composition of commercial samples of rtanj tea, Polifenolni sastav komercijalnih uzoraka rtanjskog čaja",
volume = "71",
number = "5 suplement",
pages = "S54-S55",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4669"
}

Arsenijević, J., Marčetić, M., Drobac, M., Slavkovska, V.,& Kovačević, N.. (2021). Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669

Arsenijević J, Marčetić M, Drobac M, Slavkovska V, Kovačević N. Polyphenolic composition of commercial samples of rtanj tea. in Arhiv za farmaciju. 2021;71(5 suplement):S54-S55.
https://hdl.handle.net/21.15107/rcub_farfar_4669 .

Arsenijević, Jelena, Marčetić, Mirjana, Drobac, Milica, Slavkovska, Violeta, Kovačević, Nada, "Polyphenolic composition of commercial samples of rtanj tea" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S54-S55,
https://hdl.handle.net/21.15107/rcub_farfar_4669 .

TY  - CONF
AU  - Antić, Natalija
AU  - Ilić, Milan
AU  - Marčetić, Mirjana
AU  - Drobac, Milica
AU  - Zloh, Mire
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5159
AB  - The Geronrum L. species have significant use in traditionaI medicine. Previous studies showed that these plants are rich in phytochemicals, especially in polyphenols and votatile compounds. The aim of this study was to determine potential target proteins for volatile compounds present in Geranium species and to provide a rationale for its current uses. The structures of identified volatile molecules were collated and their potential protein targets were predicted using PIDGIN software. The 2D and 3D structures of these compounds were generated using KingDraw software, while the 3D structures of selected target proteins
were obtained from Protein Data Bank. All volatile compounds were docked against the whole surface of endothelial PAS domain containing protein 1 (EPAS-1; PDBID: F310) and musarinic receptors M1 (PDBID:6WJC), M2 (PDBID:3UON), M4 (PDBID:5DG) and M5 (PDBID: 6OL9) using LeDock software. The study revealed several compounds with the potential to interact with target
proteins. Hexahydrofarnesyl acetone and γ-curcumene formed the most favorable binding poses inside the binding sites of all muscarinic receptors. Linalool and δ-guaiene forned favorable interactions to EPAS-l target protein. Predicted interaction energies are generally lower compared to ligands for these protein targts, mainly due to their size, hydrophobic properties, and the lack of H-bond forming groups. However, these compounds can fit the target site and form favorable interactions. It can be hypothesized that these plants and their extracts exert pharmacological activity via synergistic action of several components that
can interact with multiple proteins in vivo. Additionally, new therapeutic applications of these plants may be proposed based on the other targets predicted by PIDGIN but not reported in this abstract. Further in vitro studies are required to confirm in silico prediction and gain further insights on potential interactions of tested compounds and target proteins.
PB  - Sciforum
PB  - MDPI
C3  - 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online
T1  - In silico prediction of protein targets for volatile compounds of Geranium L. species
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5159
ER  - 

@conference{
author = "Antić, Natalija and Ilić, Milan and Marčetić, Mirjana and Drobac, Milica and Zloh, Mire",
year = "2021",
abstract = "The Geronrum L. species have significant use in traditionaI medicine. Previous studies showed that these plants are rich in phytochemicals, especially in polyphenols and votatile compounds. The aim of this study was to determine potential target proteins for volatile compounds present in Geranium species and to provide a rationale for its current uses. The structures of identified volatile molecules were collated and their potential protein targets were predicted using PIDGIN software. The 2D and 3D structures of these compounds were generated using KingDraw software, while the 3D structures of selected target proteins
were obtained from Protein Data Bank. All volatile compounds were docked against the whole surface of endothelial PAS domain containing protein 1 (EPAS-1; PDBID: F310) and musarinic receptors M1 (PDBID:6WJC), M2 (PDBID:3UON), M4 (PDBID:5DG) and M5 (PDBID: 6OL9) using LeDock software. The study revealed several compounds with the potential to interact with target
proteins. Hexahydrofarnesyl acetone and γ-curcumene formed the most favorable binding poses inside the binding sites of all muscarinic receptors. Linalool and δ-guaiene forned favorable interactions to EPAS-l target protein. Predicted interaction energies are generally lower compared to ligands for these protein targts, mainly due to their size, hydrophobic properties, and the lack of H-bond forming groups. However, these compounds can fit the target site and form favorable interactions. It can be hypothesized that these plants and their extracts exert pharmacological activity via synergistic action of several components that
can interact with multiple proteins in vivo. Additionally, new therapeutic applications of these plants may be proposed based on the other targets predicted by PIDGIN but not reported in this abstract. Further in vitro studies are required to confirm in silico prediction and gain further insights on potential interactions of tested compounds and target proteins.",
publisher = "Sciforum, MDPI",
journal = "25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online",
title = "In silico prediction of protein targets for volatile compounds of Geranium L. species",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5159"
}

Antić, N., Ilić, M., Marčetić, M., Drobac, M.,& Zloh, M.. (2021). In silico prediction of protein targets for volatile compounds of Geranium L. species. in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online
Sciforum..
https://hdl.handle.net/21.15107/rcub_farfar_5159

Antić N, Ilić M, Marčetić M, Drobac M, Zloh M. In silico prediction of protein targets for volatile compounds of Geranium L. species. in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online. 2021;.
https://hdl.handle.net/21.15107/rcub_farfar_5159 .

Antić, Natalija, Ilić, Milan, Marčetić, Mirjana, Drobac, Milica, Zloh, Mire, "In silico prediction of protein targets for volatile compounds of Geranium L. species" in 25th International Electronic Conference on Synthetic Organic Chemistry, 15–30 Nov 2021, online (2021),
https://hdl.handle.net/21.15107/rcub_farfar_5159 .

TY  - JOUR
AU  - Ilić, Milan D.
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan K.
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3533
AB  - Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.
PB  - Wiley-VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)
VL  - 17
IS  - 2
DO  - 10.1002/cbdv.201900544
ER  - 

@article{
author = "Ilić, Milan D. and Marčetić, Mirjana and Zlatković, Bojan K. and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2020",
abstract = "Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.",
publisher = "Wiley-VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)",
volume = "17",
number = "2",
doi = "10.1002/cbdv.201900544"
}

Ilić, M. D., Marčetić, M., Zlatković, B. K., Lakušić, B., Kovačević, N.,& Drobac, M.. (2020). Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity
Wiley-VHCA AG., 17(2).
https://doi.org/10.1002/cbdv.201900544

Ilić MD, Marčetić M, Zlatković BK, Lakušić B, Kovačević N, Drobac M. Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity. 2020;17(2).
doi:10.1002/cbdv.201900544 .

Ilić, Milan D., Marčetić, Mirjana, Zlatković, Bojan K., Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)" in Chemistry and Biodiversity, 17, no. 2 (2020),
https://doi.org/10.1002/cbdv.201900544 . .

TY  - JOUR
AU  - Ilić, Tijana
AU  - Dodevska, Margarita
AU  - Marčetić, Mirjana
AU  - Božić, Dragana
AU  - Kodranov, Igor
AU  - Vidović, Bojana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3925
AB  - Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a “superfood”
with plenty of health benefits, there is extensive research interest in their nutritional and phytochemical
composition. In the present study, the nutritional value, minerals, fatty acid composition, and bioactive
compounds of L. barbarum L., red, yellow, and black goji berry (L. ruthenicum Murray.) cultivated
in Serbia were investigated. Antioxidant and antimicrobial properties of their methanol extracts
were assessed. Red goji berry had the highest content of fats, dietary fiber, iron, total carotenoids,
and 2-O- -d-glucopyranosyl-l-ascorbic acid (AA-2 G). The yellow goji berry extract showed the
highest level of flavonoids and the most prominent antimicrobial (especially against Gram-negative
bacteria) properties. The highest total phenolic content and the most potent antioxidant activity were
observed for the extract of black goji berry. Therefore, all goji berries could be a valuable source of
bioactive compounds in the food and pharmaceutical industry.
PB  - MDPI AG
T2  - Foods
T1  - Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia
VL  - 9
IS  - 11
DO  - 10.3390/foods9111614
ER  - 

@article{
author = "Ilić, Tijana and Dodevska, Margarita and Marčetić, Mirjana and Božić, Dragana and Kodranov, Igor and Vidović, Bojana",
year = "2020",
abstract = "Since the fruits of Lycium L. species (Fructus lycii, goji berries) are promoted as a “superfood”
with plenty of health benefits, there is extensive research interest in their nutritional and phytochemical
composition. In the present study, the nutritional value, minerals, fatty acid composition, and bioactive
compounds of L. barbarum L., red, yellow, and black goji berry (L. ruthenicum Murray.) cultivated
in Serbia were investigated. Antioxidant and antimicrobial properties of their methanol extracts
were assessed. Red goji berry had the highest content of fats, dietary fiber, iron, total carotenoids,
and 2-O- -d-glucopyranosyl-l-ascorbic acid (AA-2 G). The yellow goji berry extract showed the
highest level of flavonoids and the most prominent antimicrobial (especially against Gram-negative
bacteria) properties. The highest total phenolic content and the most potent antioxidant activity were
observed for the extract of black goji berry. Therefore, all goji berries could be a valuable source of
bioactive compounds in the food and pharmaceutical industry.",
publisher = "MDPI AG",
journal = "Foods",
title = "Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia",
volume = "9",
number = "11",
doi = "10.3390/foods9111614"
}

Ilić, T., Dodevska, M., Marčetić, M., Božić, D., Kodranov, I.,& Vidović, B.. (2020). Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia. in Foods
MDPI AG., 9(11).
https://doi.org/10.3390/foods9111614

Ilić T, Dodevska M, Marčetić M, Božić D, Kodranov I, Vidović B. Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia. in Foods. 2020;9(11).
doi:10.3390/foods9111614 .

Ilić, Tijana, Dodevska, Margarita, Marčetić, Mirjana, Božić, Dragana, Kodranov, Igor, Vidović, Bojana, "Chemical characterization, antioxidant and antimicrobial properties of goji berries cultivated in Serbia" in Foods, 9, no. 11 (2020),
https://doi.org/10.3390/foods9111614 . .

TY  - JOUR
AU  - Rakić, Sveto
AU  - Janković, Snežana
AU  - Marčetić, Mirjana
AU  - Rajičić, Vera
AU  - Rakić, Radojica
AU  - Rakić, Vladimir
AU  - Kolarić, Ljubiša
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3561
AB  - The method of storage has, in the long run, a negative effect on the quality of wheat kernels, due to physiological ageing of its cells.The quality of the kernels was assessed during the storage, giving the predefined conditions - temperature 25 ± 2 C and the storage duration.Wheat kernel metabolites were studied during 12 and 24-month storage. Kernels of three commercial wheat cultivars e ‘Simonida’, ‘Dragana’ and ‘Ljiljana’, commonly grown in south-eastern Europe, were investigated. Comparing fresh samples to samples taken after 12 and 24 months of storage, it was recorded with an increase in dry matter (by 2.48% and 2.84%) and ash (by 4.09% and 15.25%), and a decrease in protein (by 8.96% and 19.81%), lipids (by 8.32% and 15.07%), starch (by 9.07% and 13.45%) and cellulose (by 22.12% and 26.35%). There were also changes in phenolics (10.51e11.82 mg GA/g). Flavonoid vitexin-glucoside (0.82e1.29 mg/ g) and flavan-3-ol catechin (1.31e1.60 mg/g), total antioxidant potential and DPPH scavenging activity were not significantly different. Organic matter digestibility (OMD) decreased, but remained at 92%. These changes did not significantly affect key properties of the kernels, OMD and antioxidant activity, thus wheat kernels can be safely stored for 24 months.
PB  - Elsevier
T2  - Journal of Stored Products Research
T1  - Functional properties of wheat kernels (Triticumaestivum L.) during storage
VL  - 87
DO  - 10.1016/j.jspr.2020.101587
ER  - 

@article{
author = "Rakić, Sveto and Janković, Snežana and Marčetić, Mirjana and Rajičić, Vera and Rakić, Radojica and Rakić, Vladimir and Kolarić, Ljubiša",
year = "2020",
abstract = "The method of storage has, in the long run, a negative effect on the quality of wheat kernels, due to physiological ageing of its cells.The quality of the kernels was assessed during the storage, giving the predefined conditions - temperature 25 ± 2 C and the storage duration.Wheat kernel metabolites were studied during 12 and 24-month storage. Kernels of three commercial wheat cultivars e ‘Simonida’, ‘Dragana’ and ‘Ljiljana’, commonly grown in south-eastern Europe, were investigated. Comparing fresh samples to samples taken after 12 and 24 months of storage, it was recorded with an increase in dry matter (by 2.48% and 2.84%) and ash (by 4.09% and 15.25%), and a decrease in protein (by 8.96% and 19.81%), lipids (by 8.32% and 15.07%), starch (by 9.07% and 13.45%) and cellulose (by 22.12% and 26.35%). There were also changes in phenolics (10.51e11.82 mg GA/g). Flavonoid vitexin-glucoside (0.82e1.29 mg/ g) and flavan-3-ol catechin (1.31e1.60 mg/g), total antioxidant potential and DPPH scavenging activity were not significantly different. Organic matter digestibility (OMD) decreased, but remained at 92%. These changes did not significantly affect key properties of the kernels, OMD and antioxidant activity, thus wheat kernels can be safely stored for 24 months.",
publisher = "Elsevier",
journal = "Journal of Stored Products Research",
title = "Functional properties of wheat kernels (Triticumaestivum L.) during storage",
volume = "87",
doi = "10.1016/j.jspr.2020.101587"
}

Rakić, S., Janković, S., Marčetić, M., Rajičić, V., Rakić, R., Rakić, V.,& Kolarić, L.. (2020). Functional properties of wheat kernels (Triticumaestivum L.) during storage. in Journal of Stored Products Research
Elsevier., 87.
https://doi.org/10.1016/j.jspr.2020.101587

Rakić S, Janković S, Marčetić M, Rajičić V, Rakić R, Rakić V, Kolarić L. Functional properties of wheat kernels (Triticumaestivum L.) during storage. in Journal of Stored Products Research. 2020;87.
doi:10.1016/j.jspr.2020.101587 .

Rakić, Sveto, Janković, Snežana, Marčetić, Mirjana, Rajičić, Vera, Rakić, Radojica, Rakić, Vladimir, Kolarić, Ljubiša, "Functional properties of wheat kernels (Triticumaestivum L.) during storage" in Journal of Stored Products Research, 87 (2020),
https://doi.org/10.1016/j.jspr.2020.101587 . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5304
AB  - Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence
SP  - 16
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5304
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2019",
abstract = "Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence",
pages = "16-16",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5304"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2019). Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):16-16,
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Obradović, Marijana
AU  - Tomić, Emilija
AU  - Teovanović, Anđelka
AU  - Lakušić, Branislava
AU  - Božić, Dragana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5303
AB  - Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria
SP  - 151
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5303
ER  - 

@conference{
author = "Marčetić, Mirjana and Obradović, Marijana and Tomić, Emilija and Teovanović, Anđelka and Lakušić, Branislava and Božić, Dragana",
year = "2019",
abstract = "Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5303"
}

Marčetić, M., Obradović, M., Tomić, E., Teovanović, A., Lakušić, B.,& Božić, D.. (2019). Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303

Marčetić M, Obradović M, Tomić E, Teovanović A, Lakušić B, Božić D. Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

Marčetić, Mirjana, Obradović, Marijana, Tomić, Emilija, Teovanović, Anđelka, Lakušić, Branislava, Božić, Dragana, "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):151-151,
https://hdl.handle.net/21.15107/rcub_farfar_5303 .